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Center for Computational Systems Medicine
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Fusion Gene Summary

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Fusion Gene ORF analysis

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Fusion Genomic Features

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Fusion Protein Features

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Fusion Gene Sequence

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Fusion Gene PPI analysis

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Related Drugs

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Related Diseases

Fusion gene:ZFAND3-RNF8 (FusionGDB2 ID:100239)

Fusion Gene Summary for ZFAND3-RNF8

check button Fusion gene summary
Fusion gene informationFusion gene name: ZFAND3-RNF8
Fusion gene ID: 100239
HgeneTgene
Gene symbol

ZFAND3

RNF8

Gene ID

60685

9025

Gene namezinc finger AN1-type containing 3ring finger protein 8
SynonymsTEX27hRNF8
Cytomap

6p21.2

6p21.2

Type of geneprotein-codingprotein-coding
DescriptionAN1-type zinc finger protein 3testis expressed sequence 27testis-expressed protein 27zinc finger, AN1-type domain 3E3 ubiquitin-protein ligase RNF8C3HC4-type zinc finger proteinRING-type E3 ubiquitin transferase RNF8UBC13/UEV-interacting ring finger proteinring finger protein (C3HC4 type) 8ring finger protein 8, E3 ubiquitin protein ligase
Modification date2020031320200313
UniProtAcc..
Ensembl transtripts involved in fusion geneENST00000287218, ENST00000373391, 
ENST00000463847, 		ENST00000373479, 
ENST00000469731, ENST00000394443, 
ENST00000479516, 
Fusion gene scores* DoF score39 X 11 X 14=60062 X 3 X 3=18
# samples 434
** MAII scorelog2(43/6006*10)=-3.80399590996769
possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs).
DoF>8 and MAII<0		log2(4/18*10)=1.15200309344505
effective Gene in Pan-Cancer Fusion Genes (eGinPCFGs).
DoF>8 and MAII>0
Context

PubMed: ZFAND3 [Title/Abstract] AND RNF8 [Title/Abstract] AND fusion [Title/Abstract]

Most frequent breakpointZFAND3(37787792)-RNF8(37328222), # samples:2
Anticipated loss of major functional domain due to fusion event.ZFAND3-RNF8 seems lost the major protein functional domain in Hgene partner, which is a essential gene due to the frame-shifted ORF.
ZFAND3-RNF8 seems lost the major protein functional domain in Tgene partner, which is a tumor suppressor due to the frame-shifted ORF.
ZFAND3-RNF8 seems lost the major protein functional domain in Tgene partner, which is a essential gene due to the frame-shifted ORF.
ZFAND3-RNF8 seems lost the major protein functional domain in Tgene partner, which is a epigenetic factor due to the frame-shifted ORF.
* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

check button Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez
PartnerGeneGO IDGO termPubMed ID
TgeneRNF8

GO:0006302

double-strand break repair

18001824|18001825|22980979

TgeneRNF8

GO:0006511

ubiquitin-dependent protein catabolic process

22266820|22373579

TgeneRNF8

GO:0006974

cellular response to DNA damage stimulus

20550933|21911360|22266820|22373579|22980979

TgeneRNF8

GO:0010212

response to ionizing radiation

18001824|18001825

TgeneRNF8

GO:0033522

histone H2A ubiquitination

18001824|18001825

TgeneRNF8

GO:0045739

positive regulation of DNA repair

18001824|18001825

TgeneRNF8

GO:0051865

protein autoubiquitination

22266820

TgeneRNF8

GO:0070534

protein K63-linked ubiquitination

21911360

TgeneRNF8

GO:0070535

histone H2A K63-linked ubiquitination

22980979

TgeneRNF8

GO:0070936

protein K48-linked ubiquitination

21911360|22266820|22373579


check buttonFusion gene breakpoints across ZFAND3 (5'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

check buttonFusion gene breakpoints across RNF8 (3'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

check button Fusion gene information from two resources (ChiTars 5.0 and ChimerDB 4.0)
* All genome coordinats were lifted-over on hg19.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.
SourceDiseaseSampleHgeneHchrHbpHstrandTgeneTchrTbpTstrand
ChimerDB4BRCATCGA-C8-A26X-01AZFAND3chr6

38029551

+RNF8chr6

37328222

+
ChimerDB4BRCATCGA-PE-A5DC-01AZFAND3chr6

37787792

+RNF8chr6

37328222

+
ChimerDB4MESOTCGA-NQ-A57I-01AZFAND3chr6

37787792

+RNF8chr6

37358518

+
ChimerDB4BRCATCGA-PE-A5DC-01AZFAND3chr6

37787792

-RNF8chr6

37328222

+


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Fusion Gene ORF analysis for ZFAND3-RNF8

check button Open reading frame (ORF) analsis of fusion genes based on Ensembl gene isoform structure.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.
ORFHenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrand
Frame-shiftENST00000287218ENST00000373479ZFAND3chr6

38029551

+RNF8chr6

37328222

+
Frame-shiftENST00000287218ENST00000469731ZFAND3chr6

38029551

+RNF8chr6

37328222

+
Frame-shiftENST00000287218ENST00000394443ZFAND3chr6

38029551

+RNF8chr6

37328222

+
5CDS-3UTRENST00000287218ENST00000479516ZFAND3chr6

38029551

+RNF8chr6

37328222

+
Frame-shiftENST00000373391ENST00000373479ZFAND3chr6

38029551

+RNF8chr6

37328222

+
Frame-shiftENST00000373391ENST00000469731ZFAND3chr6

38029551

+RNF8chr6

37328222

+
Frame-shiftENST00000373391ENST00000394443ZFAND3chr6

38029551

+RNF8chr6

37328222

+
5CDS-3UTRENST00000373391ENST00000479516ZFAND3chr6

38029551

+RNF8chr6

37328222

+
intron-3CDSENST00000463847ENST00000373479ZFAND3chr6

38029551

+RNF8chr6

37328222

+
intron-3CDSENST00000463847ENST00000469731ZFAND3chr6

38029551

+RNF8chr6

37328222

+
intron-3CDSENST00000463847ENST00000394443ZFAND3chr6

38029551

+RNF8chr6

37328222

+
intron-3UTRENST00000463847ENST00000479516ZFAND3chr6

38029551

+RNF8chr6

37328222

+
Frame-shiftENST00000287218ENST00000373479ZFAND3chr6

37787792

+RNF8chr6

37328222

+
Frame-shiftENST00000287218ENST00000469731ZFAND3chr6

37787792

+RNF8chr6

37328222

+
Frame-shiftENST00000287218ENST00000394443ZFAND3chr6

37787792

+RNF8chr6

37328222

+
5CDS-3UTRENST00000287218ENST00000479516ZFAND3chr6

37787792

+RNF8chr6

37328222

+
Frame-shiftENST00000373391ENST00000373479ZFAND3chr6

37787792

+RNF8chr6

37328222

+
Frame-shiftENST00000373391ENST00000469731ZFAND3chr6

37787792

+RNF8chr6

37328222

+
Frame-shiftENST00000373391ENST00000394443ZFAND3chr6

37787792

+RNF8chr6

37328222

+
5CDS-3UTRENST00000373391ENST00000479516ZFAND3chr6

37787792

+RNF8chr6

37328222

+
intron-3CDSENST00000463847ENST00000373479ZFAND3chr6

37787792

+RNF8chr6

37328222

+
intron-3CDSENST00000463847ENST00000469731ZFAND3chr6

37787792

+RNF8chr6

37328222

+
intron-3CDSENST00000463847ENST00000394443ZFAND3chr6

37787792

+RNF8chr6

37328222

+
intron-3UTRENST00000463847ENST00000479516ZFAND3chr6

37787792

+RNF8chr6

37328222

+
Frame-shiftENST00000287218ENST00000373479ZFAND3chr6

37787792

+RNF8chr6

37358518

+
In-frameENST00000287218ENST00000469731ZFAND3chr6

37787792

+RNF8chr6

37358518

+
5CDS-intronENST00000287218ENST00000394443ZFAND3chr6

37787792

+RNF8chr6

37358518

+
5CDS-intronENST00000287218ENST00000479516ZFAND3chr6

37787792

+RNF8chr6

37358518

+
Frame-shiftENST00000373391ENST00000373479ZFAND3chr6

37787792

+RNF8chr6

37358518

+
In-frameENST00000373391ENST00000469731ZFAND3chr6

37787792

+RNF8chr6

37358518

+
5CDS-intronENST00000373391ENST00000394443ZFAND3chr6

37787792

+RNF8chr6

37358518

+
5CDS-intronENST00000373391ENST00000479516ZFAND3chr6

37787792

+RNF8chr6

37358518

+
intron-3CDSENST00000463847ENST00000373479ZFAND3chr6

37787792

+RNF8chr6

37358518

+
intron-3CDSENST00000463847ENST00000469731ZFAND3chr6

37787792

+RNF8chr6

37358518

+
intron-intronENST00000463847ENST00000394443ZFAND3chr6

37787792

+RNF8chr6

37358518

+
intron-intronENST00000463847ENST00000479516ZFAND3chr6

37787792

+RNF8chr6

37358518

+

check buttonORFfinder result based on the fusion transcript sequence of in-frame fusion genes.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandSeq length
(transcript)		BP loci
(transcript)		Predicted start
(transcript)		Predicted stop
(transcript)		Seq length
(amino acids)
ENST00000287218ZFAND3chr637787792+ENST00000469731RNF8chr637358518+100851865627209
ENST00000373391ZFAND3chr637787792+ENST00000469731RNF8chr637358518+7092193571119

check buttonDeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandNo-coding scoreCoding score
ENST00000287218ENST00000469731ZFAND3chr637787792+RNF8chr637358518+0.946257230.053742826
ENST00000373391ENST00000469731ZFAND3chr637787792+RNF8chr637358518+0.8972420.102758035

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Fusion Genomic Features for ZFAND3-RNF8


check buttonFusionAI prediction of the potential fusion gene breakpoint based on the pre-mature RNA sequence context (+/- 5kb of individual partner genes, total 20kb length sequence). FusionAI is a fusion gene breakpoint classifier based on convolutional neural network by comparing the fusion positive and negative sequence context of ~ 20K fusion gene data. From here, we can have the relative potentency of the 20K genomic sequence how individual sequnce will be likely used as the gene fusion breakpoints.
HgeneHchrHbpHstrandTgeneTchrTbpTstrand1-pp (fusion gene breakpoint)
ZFAND3chr638029551+RNF8chr637328221+0.0093650530.990635
ZFAND3chr637787792+RNF8chr637328221+4.30E-070.9999995
ZFAND3chr637787792+RNF8chr637358517+1.20E-081
ZFAND3chr638029551+RNF8chr637328221+0.0093650530.990635
ZFAND3chr637787792+RNF8chr637328221+4.30E-070.9999995
ZFAND3chr637787792+RNF8chr637358517+1.20E-081

check buttonDistribution of 44 human genomic features loci across 20kb length fusion breakpoint regions. We integrated a total of 44 different types of human genomic feature loci information across five big categories including virus integration sites, repeats, structural variants, chromatin states, and gene expression regulation. More details are in help page.

check buttonDistribution of 44 human genomic features loci across 20kb length fusion breakpoint regions that are ovelapped with the top 1% feature importance score regions. More details are in help page.

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Fusion Protein Features for ZFAND3-RNF8


check button Go to

FGviewer for the breakpoints of chr6:37787792-chr6:37358518

.
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
FGviewer

check buttonMain function of each fusion partner protein. (from UniProt)
HgeneTgene
..
FUNCTION: Might normally function as a transcriptional repressor. EWS-fusion-proteins (EFPS) may play a role in the tumorigenic process. They may disturb gene expression by mimicking, or interfering with the normal function of CTD-POLII within the transcription initiation complex. They may also contribute to an aberrant activation of the fusion protein target genes.FUNCTION: Might normally function as a transcriptional repressor. EWS-fusion-proteins (EFPS) may play a role in the tumorigenic process. They may disturb gene expression by mimicking, or interfering with the normal function of CTD-POLII within the transcription initiation complex. They may also contribute to an aberrant activation of the fusion protein target genes.

check buttonRetention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at

download page


* Minus value of BPloci means that the break pointn is located before the CDS.
- In-frame and retained protein feature among the 13 regional features.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note
TgeneRNF8chr6:37787792chr6:37358518ENST0000039444303276_345099.0Compositional biasNote=Gln-rich
TgeneRNF8chr6:37787792chr6:37358518ENST000003944430338_92099.0DomainFHA
TgeneRNF8chr6:37787792chr6:37358518ENST0000039444303403_441099.0Zinc fingerRING-type

- In-frame and not-retained protein feature among the 13 regional features.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note
HgeneZFAND3chr6:37787792chr6:37358518ENST00000287218+1612_4423.666666666666668228.0Zinc fingerA20-type
HgeneZFAND3chr6:37787792chr6:37358518ENST00000287218+16154_20023.666666666666668228.0Zinc fingerAN1-type
TgeneRNF8chr6:37787792chr6:37358518ENST0000037347968276_345480.3333333333333486.0Compositional biasNote=Gln-rich
TgeneRNF8chr6:37787792chr6:37358518ENST0000046973157276_345412.0449.0Compositional biasNote=Gln-rich
TgeneRNF8chr6:37787792chr6:37358518ENST000003734796838_92480.3333333333333486.0DomainFHA
TgeneRNF8chr6:37787792chr6:37358518ENST000004697315738_92412.0449.0DomainFHA
TgeneRNF8chr6:37787792chr6:37358518ENST0000037347968403_441480.3333333333333486.0Zinc fingerRING-type
TgeneRNF8chr6:37787792chr6:37358518ENST0000046973157403_441412.0449.0Zinc fingerRING-type


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Fusion Gene Sequence for ZFAND3-RNF8


check button For in-frame fusion transcripts, we provide the fusion transcript sequences and fusion amino acid sequences. To have fusion amino acid sequence, we ran ORFfinder and chose the longest ORF among the all predicted ones.
>In-frame_ENST00000287218_ENST00000469731_TCGA-NQ-A57I-01A_ZFAND3_chr6_37787792_+_RNF8_chr6_37358518_length(transcript)=1008nt_BP=518nt
GTTACGGAGACAGCAGGGCCCGGGGCTTCAGAGCGGCCGCTGCGACTCCGGAGCCGGCGGGGGGCTCCGGTCCTTCCCTGCGCCACCGCA
CAGGACATCTCTCTGGCTGGGGAGCGGCGGTGAGACCCGCCGAGGGCGTCTGTGTCCCTCCTCCCCCGCGGTCCTCGGTGCGTCCGCACG
CCCCTCCTCCTCGGCTCCCTCCTTCTTCTCTCCGCGCCTCTCCGCCCCCTCCCCGTCTCCGCAGGCCGAGTGGTGCGGCCCGCCTCCAGC
TGACCGGCCTGGAATCCCGGCTCCGAGCCCCGGACTCGCGCCCGCCCGCGCGCCCGCTCCTTCCCCCTCCCCCCGCCCCGAGCCCCCCGA
CGCCGCCGCCACCGCCTCCTCAGAGCGGGGCCCGGGCCCAGCCGCCGCCACCGCTGCCGCCGCCGAGCTCCGCCGCCGCCGAGCACCATG
GGAGACGCTGGGAGCGAGCGCAGCAAAGCGCCCAGCCTGCCGCCTCGCTGTCCCTGCGGCTTCTGGGGCAAAGAGATTGTTCTGAAGACC
GTGCTCTAAGGGCATTTGAAAGACTGCCAGGTAGTGCGAGCCTGAGATGGTCTGGAGGATTCTCTCTAGCCGTGACTCCGCTGCTCTGAA
GGTCAACTGAGAAGTCTTGTGGGACAGAGACTTGAGTTAGGAAGCCCTCAGTCACTTGCCTTCCACGGTGGCCAGCCCTGCTGCCATCAT
TGGCTGAAGCACCACCAGGATTCACGGCACCCAACTGCTTCAGGGTACTTCGTAGACTCTGCCTCACTACATGTCGAAAGAGTTATTTGA
GTTCTCTTCTGTTTTTTTTTAATTTGTTGTTGTTGTTACTGTTTTGATACCTCGGAAACACCTCCGTTGACAGTTGTTTTGGATAGGTTG
GGTGTACCCCATGGCTGCCTCTGAAGGCAGTGTCTATTTTGAGAGGATGGCTTACCTCTTCTTTGTGAAAATACTATCTCATTTCCTGGA

>In-frame_ENST00000287218_ENST00000469731_TCGA-NQ-A57I-01A_ZFAND3_chr6_37787792_+_RNF8_chr6_37358518_length(amino acids)=209AA_start in transcript=656_stop in transcript=27
MSHKTSQLTFRAAESRLERILQTISGSHYLAVFQMPLEHGLQNNLFAPEAAGTARRQAGRFAALAPSVSHGARRRRSSAAAAVAAAGPGP
RSEEAVAAASGGSGRGEGEGAGARAGASPGLGAGIPGRSAGGGPHHSACGDGEGAERRGEKKEGAEEEGRADAPRTAGEEGHRRPRRVSP

--------------------------------------------------------------
>In-frame_ENST00000373391_ENST00000469731_TCGA-NQ-A57I-01A_ZFAND3_chr6_37787792_+_RNF8_chr6_37358518_length(transcript)=709nt_BP=219nt
CCGGACTCGCGCCCGCCCGCGCGCCCGCTCCTTCCCCCTCCCCCCGCCCCGAGCCCCCCGACGCCGCCGCCACCGCCTCCTCAGAGCGGG
GCCCGGGCCCAGCCGCCGCCACCGCTGCCGCCGCCGAGCTCCGCCGCCGCCGAGCACCATGGGAGACGCTGGGAGCGAGCGCAGCAAAGC
GCCCAGCCTGCCGCCTCGCTGTCCCTGCGGCTTCTGGGGCAAAGAGATTGTTCTGAAGACCGTGCTCTAAGGGCATTTGAAAGACTGCCA
GGTAGTGCGAGCCTGAGATGGTCTGGAGGATTCTCTCTAGCCGTGACTCCGCTGCTCTGAAGGTCAACTGAGAAGTCTTGTGGGACAGAG
ACTTGAGTTAGGAAGCCCTCAGTCACTTGCCTTCCACGGTGGCCAGCCCTGCTGCCATCATTGGCTGAAGCACCACCAGGATTCACGGCA
CCCAACTGCTTCAGGGTACTTCGTAGACTCTGCCTCACTACATGTCGAAAGAGTTATTTGAGTTCTCTTCTGTTTTTTTTTAATTTGTTG
TTGTTGTTACTGTTTTGATACCTCGGAAACACCTCCGTTGACAGTTGTTTTGGATAGGTTGGGTGTACCCCATGGCTGCCTCTGAAGGCA

>In-frame_ENST00000373391_ENST00000469731_TCGA-NQ-A57I-01A_ZFAND3_chr6_37787792_+_RNF8_chr6_37358518_length(amino acids)=119AA_start in transcript=357_stop in transcript=1
MSHKTSQLTFRAAESRLERILQTISGSHYLAVFQMPLEHGLQNNLFAPEAAGTARRQAGRFAALAPSVSHGARRRRSSAAAAVAAAGPGP

--------------------------------------------------------------

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Fusion Gene PPI Analysis for ZFAND3-RNF8


check button Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in

ChiPPI page.


check button Protein-protein interactors with each fusion partner protein in wild-type (BIOGRID-3.4.160)
HgeneHgene's interactorsTgeneTgene's interactors


check button - Retained PPIs in in-frame fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenStill interaction with


check button - Lost PPIs in in-frame fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenInteraction lost with


check button - Retained PPIs, but lost function due to frame-shift fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenInteraction lost with


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Related Drugs for ZFAND3-RNF8


check button Drugs targeting genes involved in this fusion gene.
(DrugBank Version 5.1.8 2021-05-08)
PartnerGeneUniProtAccDrugBank IDDrug nameDrug activityDrug typeDrug status

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Related Diseases for ZFAND3-RNF8


check button Diseases associated with fusion partners.
(DisGeNet 4.0)
PartnerGeneDisease IDDisease name# pubmedsSource
HgeneZFAND3C0011860Diabetes Mellitus, Non-Insulin-Dependent1CTD_human
HgeneZFAND3C0087031Juvenile-Onset Still Disease1CTD_human
HgeneZFAND3C3495559Juvenile arthritis1CTD_human
HgeneZFAND3C3714758Juvenile psoriatic arthritis1CTD_human
HgeneZFAND3C4552091Polyarthritis, Juvenile, Rheumatoid Factor Negative1CTD_human
HgeneZFAND3C4704862Polyarthritis, Juvenile, Rheumatoid Factor Positive1CTD_human
TgeneRNF8C0004352Autistic Disorder1CTD_human