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Center for Computational Systems Medicine
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Fusion Gene Summary

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Fusion Gene ORF analysis

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Fusion Genomic Features

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Fusion Protein Features

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Fusion Gene Sequence

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Fusion Gene PPI analysis

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Related Drugs

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Related Diseases

Fusion gene:CEP135-POLR2B (FusionGDB2 ID:15812)

Fusion Gene Summary for CEP135-POLR2B

check button Fusion gene summary
Fusion gene informationFusion gene name: CEP135-POLR2B
Fusion gene ID: 15812
HgeneTgene
Gene symbol

CEP135

POLR2B

Gene ID

9662

5431

Gene namecentrosomal protein 135RNA polymerase II subunit B
SynonymsCEP4|KIAA0635|MCPH8POL2RB|RPB2|hRPB140
Cytomap

4q12

4q12

Type of geneprotein-codingprotein-coding
Descriptioncentrosomal protein of 135 kDacentrosomal protein 135kDacentrosomal protein 4centrosome protein cep135DNA-directed RNA polymerase II subunit RPB2DNA-directed RNA polymerase II 140 kDa polypeptideDNA-directed RNA polymerase II subunit BRNA polymerase II second largest subunitRNA polymerase II subunit 2RNA polymerase II subunit B2polymerase (RNA) II (
Modification date2020032820200313
UniProtAcc

Q66GS9

.
Ensembl transtripts involved in fusion geneENST00000257287, ENST00000422247, 
ENST00000381227, ENST00000431623, 
ENST00000441246, ENST00000314595, 
ENST00000510355, 
Fusion gene scores* DoF score4 X 3 X 4=484 X 4 X 2=32
# samples 44
** MAII scorelog2(4/48*10)=-0.263034405833794
possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs).
DoF>8 and MAII<0		log2(4/32*10)=0.321928094887362
effective Gene in Pan-Cancer Fusion Genes (eGinPCFGs).
DoF>8 and MAII>0
Context

PubMed: CEP135 [Title/Abstract] AND POLR2B [Title/Abstract] AND fusion [Title/Abstract]

Most frequent breakpointPOLR2B(57861575)-CEP135(56851447), # samples:2
CEP135(56847545)-POLR2B(57865783), # samples:2
Anticipated loss of major functional domain due to fusion event.
* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

check button Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez
PartnerGeneGO IDGO termPubMed ID
TgenePOLR2B

GO:0006366

transcription by RNA polymerase II

9852112


check buttonFusion gene breakpoints across CEP135 (5'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

check buttonFusion gene breakpoints across POLR2B (3'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

check button Fusion gene information from two resources (ChiTars 5.0 and ChimerDB 4.0)
* All genome coordinats were lifted-over on hg19.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.
SourceDiseaseSampleHgeneHchrHbpHstrandTgeneTchrTbpTstrand
ChimerDB4LGGTCGA-S9-A89Z-01ACEP135chr4

56847545

+POLR2Bchr4

57865783

+
ChimerDB4LGGTCGA-S9-A89Z-01ACEP135chr4

56847545

-POLR2Bchr4

57865783

+


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Fusion Gene ORF analysis for CEP135-POLR2B

check button Open reading frame (ORF) analsis of fusion genes based on Ensembl gene isoform structure.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.
ORFHenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrand
In-frameENST00000257287ENST00000381227CEP135chr4

56847545

+POLR2Bchr4

57865783

+
In-frameENST00000257287ENST00000431623CEP135chr4

56847545

+POLR2Bchr4

57865783

+
In-frameENST00000257287ENST00000441246CEP135chr4

56847545

+POLR2Bchr4

57865783

+
In-frameENST00000257287ENST00000314595CEP135chr4

56847545

+POLR2Bchr4

57865783

+
5CDS-intronENST00000257287ENST00000510355CEP135chr4

56847545

+POLR2Bchr4

57865783

+
intron-3CDSENST00000422247ENST00000381227CEP135chr4

56847545

+POLR2Bchr4

57865783

+
intron-3CDSENST00000422247ENST00000431623CEP135chr4

56847545

+POLR2Bchr4

57865783

+
intron-3CDSENST00000422247ENST00000441246CEP135chr4

56847545

+POLR2Bchr4

57865783

+
intron-3CDSENST00000422247ENST00000314595CEP135chr4

56847545

+POLR2Bchr4

57865783

+
intron-intronENST00000422247ENST00000510355CEP135chr4

56847545

+POLR2Bchr4

57865783

+

check buttonORFfinder result based on the fusion transcript sequence of in-frame fusion genes.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandSeq length
(transcript)		BP loci
(transcript)		Predicted start
(transcript)		Predicted stop
(transcript)		Seq length
(amino acids)
ENST00000257287CEP135chr456847545+ENST00000381227POLR2Bchr457865783+4863190312446921522
ENST00000257287CEP135chr456847545+ENST00000431623POLR2Bchr457865783+4863190312446921522
ENST00000257287CEP135chr456847545+ENST00000441246POLR2Bchr457865783+4712190312446921522
ENST00000257287CEP135chr456847545+ENST00000314595POLR2Bchr457865783+4873190312446921522

check buttonDeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandNo-coding scoreCoding score
ENST00000257287ENST00000381227CEP135chr456847545+POLR2Bchr457865783+0.0004568920.9995432
ENST00000257287ENST00000431623CEP135chr456847545+POLR2Bchr457865783+0.0004568920.9995432
ENST00000257287ENST00000441246CEP135chr456847545+POLR2Bchr457865783+0.0006570060.999343
ENST00000257287ENST00000314595CEP135chr456847545+POLR2Bchr457865783+0.0004383780.9995616

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Fusion Genomic Features for CEP135-POLR2B


check buttonFusionAI prediction of the potential fusion gene breakpoint based on the pre-mature RNA sequence context (+/- 5kb of individual partner genes, total 20kb length sequence). FusionAI is a fusion gene breakpoint classifier based on convolutional neural network by comparing the fusion positive and negative sequence context of ~ 20K fusion gene data. From here, we can have the relative potentency of the 20K genomic sequence how individual sequnce will be likely used as the gene fusion breakpoints.
HgeneHchrHbpHstrandTgeneTchrTbpTstrand1-pp (fusion gene breakpoint)
CEP135chr456847545+POLR2Bchr457865782+9.22E-050.99990773
CEP135chr456847545+POLR2Bchr457865782+9.22E-050.99990773

check buttonDistribution of 44 human genomic features loci across 20kb length fusion breakpoint regions. We integrated a total of 44 different types of human genomic feature loci information across five big categories including virus integration sites, repeats, structural variants, chromatin states, and gene expression regulation. More details are in help page.

check buttonDistribution of 44 human genomic features loci across 20kb length fusion breakpoint regions that are ovelapped with the top 1% feature importance score regions. More details are in help page.

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Fusion Protein Features for CEP135-POLR2B


check button Go to

FGviewer for the breakpoints of chr4:56847545-chr4:57865783

.
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
FGviewer

check buttonMain function of each fusion partner protein. (from UniProt)
HgeneTgene
CEP135

Q66GS9

.
FUNCTION: Centrosomal protein involved in centriole biogenesis. Acts as a scaffolding protein during early centriole biogenesis. Required for the targeting of centriole satellite proteins to centrosomes such as of PCM1, SSX2IP and CEP290 and recruitment of WRAP73 to centrioles. Also required for centriole-centriole cohesion during interphase by acting as a platform protein for CEP250 at the centriole. Required for the recruitment of CEP295 to the proximal end of new-born centrioles at the centriolar microtubule wall during early S phase in a PLK4-dependent manner. Required for the recruitment of CEP295 to the proximal end of new-born centrioles at the centriolar microtubule wall during early S phase in a PLK4-dependent manner (PubMed:27185865). {ECO:0000269|PubMed:17681131, ECO:0000269|PubMed:18851962, ECO:0000269|PubMed:26675238, ECO:0000269|PubMed:27185865}.FUNCTION: Might normally function as a transcriptional repressor. EWS-fusion-proteins (EFPS) may play a role in the tumorigenic process. They may disturb gene expression by mimicking, or interfering with the normal function of CTD-POLII within the transcription initiation complex. They may also contribute to an aberrant activation of the fusion protein target genes.

check buttonRetention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at

download page


* Minus value of BPloci means that the break pointn is located before the CDS.
- In-frame and retained protein feature among the 13 regional features.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note
HgeneCEP135chr4:56847545chr4:57865783ENST00000257287+1326195_420593.01782.6666666666667Coiled coilOntology_term=ECO:0000255
HgeneCEP135chr4:56847545chr4:57865783ENST00000257287+132671_152593.01782.6666666666667Coiled coilOntology_term=ECO:0000255
TgenePOLR2Bchr4:56847545chr4:57865783ENST000003145955251119_1140245.01175.0Zinc fingerNote=C4-type
TgenePOLR2Bchr4:56847545chr4:57865783ENST000003812276261119_1140245.01175.0Zinc fingerNote=C4-type

- In-frame and not-retained protein feature among the 13 regional features.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note
HgeneCEP135chr4:56847545chr4:57865783ENST00000257287+13261069_1116593.01782.6666666666667Coiled coilOntology_term=ECO:0000255
HgeneCEP135chr4:56847545chr4:57865783ENST00000257287+1326447_1039593.01782.6666666666667Coiled coilOntology_term=ECO:0000255
HgeneCEP135chr4:56847545chr4:57865783ENST00000422247+161069_11160250.0Coiled coilOntology_term=ECO:0000255
HgeneCEP135chr4:56847545chr4:57865783ENST00000422247+16195_4200250.0Coiled coilOntology_term=ECO:0000255
HgeneCEP135chr4:56847545chr4:57865783ENST00000422247+16447_10390250.0Coiled coilOntology_term=ECO:0000255
HgeneCEP135chr4:56847545chr4:57865783ENST00000422247+1671_1520250.0Coiled coilOntology_term=ECO:0000255


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Fusion Gene Sequence for CEP135-POLR2B


check button For in-frame fusion transcripts, we provide the fusion transcript sequences and fusion amino acid sequences. To have fusion amino acid sequence, we ran ORFfinder and chose the longest ORF among the all predicted ones.
>In-frame_ENST00000257287_ENST00000381227_TCGA-S9-A89Z-01A_CEP135_chr4_56847545_+_POLR2B_chr4_57865783_length(transcript)=4863nt_BP=1903nt
GACGGAAGGAGCTAGTGGGGGACTCGAGGCCTGAGGGCAATGCGGCTGGAGGCGGAGGCAACGGCGGCTGGAGCTGCCGGACTTTAATTT
TTGGAAGTGAATAAAACTTGTTTTAGAAGACGAGATGACTACAGCTGTAGAGAGAAAGTATATTAATATTAGGAAAAGGCTGGATCAGCT
GGGATACCGCCAGACTCTGACAGTGGAGTGTTTACCTTTGGTAGAAAAACTTTTCAGCGACTTAGTTCATACAACTGAGAGCCTTCGGCA
ATCAAAATTATCTGCTGTGAAAGCTGAAAAAGAAAGTGCCAATTTTGATTTTGTTTTGGAACCCTATAAACTTGAAAATGCAAGATTGAG
TAGAGAAAATAATGAATTATACCTAGAGTTAATGAAACTGAGAGAACATTCAGACCAACACGTTAAAGAGTTGAAAACTTCATTGAAGAA
ATGTGCACGTGAAACAGCTGATCTGAAATTTCTGAATAACCAATATGCTCATAAACTCAAACTGTTGGAGAAAGAGAGCAAAGCTAAGAA
TGAAAGAATTCAACAACTTCAAGAAAAGAATTTGCATGCTGTAGTACAAACTCCAGGTGGCAAGAAAAGAAGTATTGCTTTCAGGCGCCA
GCGTATGCAAATTGATGAACCGGTTCCTCCCTCTGAAGTCAGTTCATATCCAGTTCCTCAACCAGATGACCCTTACATTGCAGACCTCCT
TCAAGTGGCTGATAACAGGATTCAAGAACTTCAACAGGAAGTCCACCAGCTACAAGAAAAGTTAGCAATGATGGAAAGTGGGGTGAGAGA
CTATAGCAAGCAGATTGAGCTAAGAGAACGAGAGATAGAACGACTGTCAGTTGCTTTGGATGGTGGTCGGTCCCCTGATGTCCTTTCTCT
GGAGTCTAGAAATAAAACCAATGAAAAGCTTATTGCTCATTTAAATATTCAGGTTGACTTTCTTCAGCAAGCTAATAAAGACCTGGAGAA
GCGTATACGAGAGCTTATGGAAACCAAGGAAACAGTGACATCTGAAGTCGTTAATTTAAGTAACAAAAATGAAAAACTCTGCCAAGAATT
AACTGAAATAGATCAGTTAGCACAGCAGTTGGAAAGACATAAAGAAGAAGTGCTTGAGACTGCTGATAAAGAGCTTGGGGAAGCAAAGAA
AGAGATTAAAAGAAAGCTCTCTGAAATGCAGGATCTTGAAGAAACAATGGCAAAACTTCAGCTGGAATTGAACTTATGCCAGAAAGAAAA
GGAGAGACTGAGTGATGAACTCCTTGTAAAATCAGACCTAGAAACTGTTGTTCATCAGCTTGAACAAGAAAAGCAAAGACTTAGCAAAAA
AGTTGAAAGTTTTGCAGTTACAGAACGACAACTTACTCTGGAGGTTGAGAGGATGAGACTAGAACATGGAATAAAACGTCGAGACAGGTC
ACCTTCTCGTTTAGATACATTTCTGAAAGGTATAGAAGAAGAACGAGATTATTATAAGAAAGAGCTAGAGAGACTCCAACATATAATACA
GCGAAGATCTTGCTCTACAAGTTATAGCGCACGTGAAAAAAGTTCAATATTTAGAACACCAGAAAAGGGTGATTACAATTCAGAAATTCA
TCAGATCACAAGAGAAAGAGATGAACTTCAGCGTATGCTAGAAAGATTTGAAAAATATATGGAAGATATACAGTCCAATGTTAAATTATT
GACAGCAGAAAGAGATAAACTAAGTGTCTTATATAATGAAGCTCAGGAAGAATTATCTGCCCTAAGAAAGGAATCCACCCAAACCACAGC
ACCCCATAATATTGTTAGTCTTATGGAAAAGGAAAAAGAACTTGCGTTATCTGACTTAAGAAGAATTATGGCAGAAAAGGAAGCTTTAAG
AGAAAAATTAGAGGGTGCCAAGAAGAGTGCTATTGGTCAGCGCATTGTGGCAACTCTACCATATATCAAGCAAGAAGTTCCCATCATTAT
TGTGTTCAGAGCATTAGGTTTTGTGTCCGACAGAGATATTTTAGAACATATTATTTATGATTTTGAAGATCCAGAGATGATGGAAATGGT
TAAACCTTCTCTCGATGAAGCTTTTGTCATCCAAGAACAGAATGTTGCACTAAATTTCATTGGTTCAAGAGGAGCAAAGCCTGGTGTTAC
TAAAGAGAAAAGAATTAAATATGCAAAGGAAGTTTTACAAAAAGAAATGCTCCCTCATGTTGGTGTCAGTGATTTTTGTGAGACCAAAAA
AGCCTATTTCTTGGGATACATGGTTCATAGGTTACTTCTGGCAGCTTTGGGTAGAAGAGAACTAGATGACAGAGATCACTATGGAAACAA
GAGATTGGATCTTGCTGGGCCGCTGCTTGCATTCTTATTTAGAGGTATGTTTAAGAATTTGCTTAAAGAAGTGCGGATCTATGCACAGAA
ATTTATTGATCGAGGAAAGGATTTTAACTTGGAGTTGGCAATTAAAACACGGATCATATCTGATGGCCTAAAATACTCTTTAGCTACTGG
AAACTGGGGTGATCAAAAGAAAGCTCATCAAGCCAGAGCTGGAGTATCTCAGGTGTTAAACCGCCTGACTTTTGCGTCTACTCTTTCTCA
CCTGCGTCGTTTAAATTCTCCTATTGGTAGAGACGGCAAGCTAGCAAAACCAAGACAGTTGCATAATACGTTGTGGGGAATGGTGTGTCC
TGCCGAGACCCCAGAGGGCCATGCTGTAGGACTTGTGAAGAATTTAGCCTTGATGGCGTATATTTCAGTTGGATCTCAACCATCTCCAAT
TCTGGAATTTTTAGAAGAATGGAGTATGGAAAATTTAGAAGAAATTTCTCCTGCAGCTATTGCTGATGCAACCAAGATTTTTGTTAATGG
CTGCTGGGTTGGAATACATAAAGATCCCGAACAACTTATGAACACCCTAAGGAAATTGAGACGTCAGATGGACATCATTGTGTCTGAAGT
TTCTATGATCAGAGATATTCGAGAGAGGGAGATTCGGATCTATACGGATGCAGGCCGTATTTGTAGACCACTTCTGATTGTGGAAAAACA
AAAGCTACTTTTGAAGAAGAGGCATATTGACCAATTGAAAGAGAGAGAATATAACAACTATAGTTGGCAGGATCTTGTGGCCAGTGGGGT
AGTGGAGTATATTGATACCCTGGAAGAAGAAACAGTGATGCTTGCAATGACTCCAGATGATTTACAGGAGAAAGAAGTAGCTTATTGTTC
CACATATACACACTGTGAGATTCATCCCTCAATGATCCTTGGTGTCTGTGCATCTATTATTCCCTTTCCTGATCATAACCAGTCCCCTAG
AAACACATACCAGTCTGCTATGGGTAAGCAGGCTATGGGAGTTTACATCACCAACTTCCATGTTCGCATGGACACATTGGCCCATGTTCT
CTATTATCCTCAAAAGCCACTTGTGACTACACGGTCTATGGAATATCTACGATTTAGAGAGCTGCCAGCAGGCATCAACTCAATTGTGGC
CATTGCATCATACACTGGATATAATCAGGAAGACTCTGTTATCATGAATCGTTCAGCTGTAGACCGCGGCTTCTTCAGGTCTGTTTTCTA
TCGCTCATACAAAGAACAGGAGTCTAAAAAAGGATTTGATCAAGAAGAAGTTTTTGAGAAGCCTACACGTGAAACATGCCAGGGCATGAG
GCATGCCATTTACGACAAGCTGGATGATGATGGTTTGATAGCTCCAGGGGTTCGTGTATCAGGAGATGATGTTATTATAGGCAAAACAGT
CACCTTGCCTGAAAATGAAGATGAATTGGAGAGCACCAATAGACGCTATACCAAGAGAGACTGTAGCACTTTTCTCAGAACTAGCGAGAC
GGGCATTGTGGATCAGGTTATGGTAACTCTCAATCAGGAAGGATATAAATTTTGTAAAATAAGGGTACGCTCTGTTAGGATTCCACAGAT
TGGAGACAAATTTGCTAGTCGACATGGTCAAAAGGGTACTTGTGGTATTCAGTATAGACAAGAGGATATGCCTTTCACCTGTGAAGGTAT
CACCCCTGATATCATCATCAATCCCCATGCCATCCCCTCTCGTATGACTATTGGTCACTTAATTGAATGCCTTCAAGGGAAGGTATCGGC
TAACAAGGGTGAAATTGGTGATGCCACTCCATTTAATGATGCTGTTAACGTGCAGAAGATTTCTAATCTTTTATCTGATTATGGCTATCA
TCTCAGAGGAAATGAGGTCCTGTACAATGGGTTCACTGGTCGAAAAATCACATCACAAATATTTATTGGCCCCACTTATTACCAGCGTTT
GAAGCATATGGTGGATGATAAGATTCACTCTCGTGCTAGGGGACCTATTCAGATCCTCAATAGACAGCCCATGGAGGGTAGATCTCGTGA
TGGTGGCCTGCGTTTTGGAGAAATGGAACGAGATTGTCAGATTGCCCATGGAGCAGCCCAGTTTTTAAGGGAAAGATTGTTTGAGGCATC
AGATCCATATCAGGTTCATGTTTGCAATCTTTGTGGAATAATGGCGATTGCCAACACCAGGACCCATACATATGAATGCAGGGGCTGCCG
CAATAAAACCCAGATTTCTTTGGTGCGAATGCCTTACGCATGCAAACTATTGTTTCAGGAACTTATGTCTATGAGTATTGCACCGCGAAT
GATGAGTGTTTAGCTATTTTACAGGAGTCAACAAGATAATTAAATATCTTGGTGTCTTGTTTCTATTGTGTGGCTTTTTAAAAATGACAA
ATATGTACTGTGTTGTGATAAAAAGTATTTTATTTGTTTAATGATATGCATGCTTTTCTTCTGTAAATATATAATAAATTTTTGTAGATA

>In-frame_ENST00000257287_ENST00000381227_TCGA-S9-A89Z-01A_CEP135_chr4_56847545_+_POLR2B_chr4_57865783_length(amino acids)=1522AA_start in transcript=124_stop in transcript=4692
MTTAVERKYINIRKRLDQLGYRQTLTVECLPLVEKLFSDLVHTTESLRQSKLSAVKAEKESANFDFVLEPYKLENARLSRENNELYLELM
KLREHSDQHVKELKTSLKKCARETADLKFLNNQYAHKLKLLEKESKAKNERIQQLQEKNLHAVVQTPGGKKRSIAFRRQRMQIDEPVPPS
EVSSYPVPQPDDPYIADLLQVADNRIQELQQEVHQLQEKLAMMESGVRDYSKQIELREREIERLSVALDGGRSPDVLSLESRNKTNEKLI
AHLNIQVDFLQQANKDLEKRIRELMETKETVTSEVVNLSNKNEKLCQELTEIDQLAQQLERHKEEVLETADKELGEAKKEIKRKLSEMQD
LEETMAKLQLELNLCQKEKERLSDELLVKSDLETVVHQLEQEKQRLSKKVESFAVTERQLTLEVERMRLEHGIKRRDRSPSRLDTFLKGI
EEERDYYKKELERLQHIIQRRSCSTSYSAREKSSIFRTPEKGDYNSEIHQITRERDELQRMLERFEKYMEDIQSNVKLLTAERDKLSVLY
NEAQEELSALRKESTQTTAPHNIVSLMEKEKELALSDLRRIMAEKEALREKLEGAKKSAIGQRIVATLPYIKQEVPIIIVFRALGFVSDR
DILEHIIYDFEDPEMMEMVKPSLDEAFVIQEQNVALNFIGSRGAKPGVTKEKRIKYAKEVLQKEMLPHVGVSDFCETKKAYFLGYMVHRL
LLAALGRRELDDRDHYGNKRLDLAGPLLAFLFRGMFKNLLKEVRIYAQKFIDRGKDFNLELAIKTRIISDGLKYSLATGNWGDQKKAHQA
RAGVSQVLNRLTFASTLSHLRRLNSPIGRDGKLAKPRQLHNTLWGMVCPAETPEGHAVGLVKNLALMAYISVGSQPSPILEFLEEWSMEN
LEEISPAAIADATKIFVNGCWVGIHKDPEQLMNTLRKLRRQMDIIVSEVSMIRDIREREIRIYTDAGRICRPLLIVEKQKLLLKKRHIDQ
LKEREYNNYSWQDLVASGVVEYIDTLEEETVMLAMTPDDLQEKEVAYCSTYTHCEIHPSMILGVCASIIPFPDHNQSPRNTYQSAMGKQA
MGVYITNFHVRMDTLAHVLYYPQKPLVTTRSMEYLRFRELPAGINSIVAIASYTGYNQEDSVIMNRSAVDRGFFRSVFYRSYKEQESKKG
FDQEEVFEKPTRETCQGMRHAIYDKLDDDGLIAPGVRVSGDDVIIGKTVTLPENEDELESTNRRYTKRDCSTFLRTSETGIVDQVMVTLN
QEGYKFCKIRVRSVRIPQIGDKFASRHGQKGTCGIQYRQEDMPFTCEGITPDIIINPHAIPSRMTIGHLIECLQGKVSANKGEIGDATPF
NDAVNVQKISNLLSDYGYHLRGNEVLYNGFTGRKITSQIFIGPTYYQRLKHMVDDKIHSRARGPIQILNRQPMEGRSRDGGLRFGEMERD

--------------------------------------------------------------
>In-frame_ENST00000257287_ENST00000431623_TCGA-S9-A89Z-01A_CEP135_chr4_56847545_+_POLR2B_chr4_57865783_length(transcript)=4863nt_BP=1903nt
GACGGAAGGAGCTAGTGGGGGACTCGAGGCCTGAGGGCAATGCGGCTGGAGGCGGAGGCAACGGCGGCTGGAGCTGCCGGACTTTAATTT
TTGGAAGTGAATAAAACTTGTTTTAGAAGACGAGATGACTACAGCTGTAGAGAGAAAGTATATTAATATTAGGAAAAGGCTGGATCAGCT
GGGATACCGCCAGACTCTGACAGTGGAGTGTTTACCTTTGGTAGAAAAACTTTTCAGCGACTTAGTTCATACAACTGAGAGCCTTCGGCA
ATCAAAATTATCTGCTGTGAAAGCTGAAAAAGAAAGTGCCAATTTTGATTTTGTTTTGGAACCCTATAAACTTGAAAATGCAAGATTGAG
TAGAGAAAATAATGAATTATACCTAGAGTTAATGAAACTGAGAGAACATTCAGACCAACACGTTAAAGAGTTGAAAACTTCATTGAAGAA
ATGTGCACGTGAAACAGCTGATCTGAAATTTCTGAATAACCAATATGCTCATAAACTCAAACTGTTGGAGAAAGAGAGCAAAGCTAAGAA
TGAAAGAATTCAACAACTTCAAGAAAAGAATTTGCATGCTGTAGTACAAACTCCAGGTGGCAAGAAAAGAAGTATTGCTTTCAGGCGCCA
GCGTATGCAAATTGATGAACCGGTTCCTCCCTCTGAAGTCAGTTCATATCCAGTTCCTCAACCAGATGACCCTTACATTGCAGACCTCCT
TCAAGTGGCTGATAACAGGATTCAAGAACTTCAACAGGAAGTCCACCAGCTACAAGAAAAGTTAGCAATGATGGAAAGTGGGGTGAGAGA
CTATAGCAAGCAGATTGAGCTAAGAGAACGAGAGATAGAACGACTGTCAGTTGCTTTGGATGGTGGTCGGTCCCCTGATGTCCTTTCTCT
GGAGTCTAGAAATAAAACCAATGAAAAGCTTATTGCTCATTTAAATATTCAGGTTGACTTTCTTCAGCAAGCTAATAAAGACCTGGAGAA
GCGTATACGAGAGCTTATGGAAACCAAGGAAACAGTGACATCTGAAGTCGTTAATTTAAGTAACAAAAATGAAAAACTCTGCCAAGAATT
AACTGAAATAGATCAGTTAGCACAGCAGTTGGAAAGACATAAAGAAGAAGTGCTTGAGACTGCTGATAAAGAGCTTGGGGAAGCAAAGAA
AGAGATTAAAAGAAAGCTCTCTGAAATGCAGGATCTTGAAGAAACAATGGCAAAACTTCAGCTGGAATTGAACTTATGCCAGAAAGAAAA
GGAGAGACTGAGTGATGAACTCCTTGTAAAATCAGACCTAGAAACTGTTGTTCATCAGCTTGAACAAGAAAAGCAAAGACTTAGCAAAAA
AGTTGAAAGTTTTGCAGTTACAGAACGACAACTTACTCTGGAGGTTGAGAGGATGAGACTAGAACATGGAATAAAACGTCGAGACAGGTC
ACCTTCTCGTTTAGATACATTTCTGAAAGGTATAGAAGAAGAACGAGATTATTATAAGAAAGAGCTAGAGAGACTCCAACATATAATACA
GCGAAGATCTTGCTCTACAAGTTATAGCGCACGTGAAAAAAGTTCAATATTTAGAACACCAGAAAAGGGTGATTACAATTCAGAAATTCA
TCAGATCACAAGAGAAAGAGATGAACTTCAGCGTATGCTAGAAAGATTTGAAAAATATATGGAAGATATACAGTCCAATGTTAAATTATT
GACAGCAGAAAGAGATAAACTAAGTGTCTTATATAATGAAGCTCAGGAAGAATTATCTGCCCTAAGAAAGGAATCCACCCAAACCACAGC
ACCCCATAATATTGTTAGTCTTATGGAAAAGGAAAAAGAACTTGCGTTATCTGACTTAAGAAGAATTATGGCAGAAAAGGAAGCTTTAAG
AGAAAAATTAGAGGGTGCCAAGAAGAGTGCTATTGGTCAGCGCATTGTGGCAACTCTACCATATATCAAGCAAGAAGTTCCCATCATTAT
TGTGTTCAGAGCATTAGGTTTTGTGTCCGACAGAGATATTTTAGAACATATTATTTATGATTTTGAAGATCCAGAGATGATGGAAATGGT
TAAACCTTCTCTCGATGAAGCTTTTGTCATCCAAGAACAGAATGTTGCACTAAATTTCATTGGTTCAAGAGGAGCAAAGCCTGGTGTTAC
TAAAGAGAAAAGAATTAAATATGCAAAGGAAGTTTTACAAAAAGAAATGCTCCCTCATGTTGGTGTCAGTGATTTTTGTGAGACCAAAAA
AGCCTATTTCTTGGGATACATGGTTCATAGGTTACTTCTGGCAGCTTTGGGTAGAAGAGAACTAGATGACAGAGATCACTATGGAAACAA
GAGATTGGATCTTGCTGGGCCGCTGCTTGCATTCTTATTTAGAGGTATGTTTAAGAATTTGCTTAAAGAAGTGCGGATCTATGCACAGAA
ATTTATTGATCGAGGAAAGGATTTTAACTTGGAGTTGGCAATTAAAACACGGATCATATCTGATGGCCTAAAATACTCTTTAGCTACTGG
AAACTGGGGTGATCAAAAGAAAGCTCATCAAGCCAGAGCTGGAGTATCTCAGGTGTTAAACCGCCTGACTTTTGCGTCTACTCTTTCTCA
CCTGCGTCGTTTAAATTCTCCTATTGGTAGAGACGGCAAGCTAGCAAAACCAAGACAGTTGCATAATACGTTGTGGGGAATGGTGTGTCC
TGCCGAGACCCCAGAGGGCCATGCTGTAGGACTTGTGAAGAATTTAGCCTTGATGGCGTATATTTCAGTTGGATCTCAACCATCTCCAAT
TCTGGAATTTTTAGAAGAATGGAGTATGGAAAATTTAGAAGAAATTTCTCCTGCAGCTATTGCTGATGCAACCAAGATTTTTGTTAATGG
CTGCTGGGTTGGAATACATAAAGATCCCGAACAACTTATGAACACCCTAAGGAAATTGAGACGTCAGATGGACATCATTGTGTCTGAAGT
TTCTATGATCAGAGATATTCGAGAGAGGGAGATTCGGATCTATACGGATGCAGGCCGTATTTGTAGACCACTTCTGATTGTGGAAAAACA
AAAGCTACTTTTGAAGAAGAGGCATATTGACCAATTGAAAGAGAGAGAATATAACAACTATAGTTGGCAGGATCTTGTGGCCAGTGGGGT
AGTGGAGTATATTGATACCCTGGAAGAAGAAACAGTGATGCTTGCAATGACTCCAGATGATTTACAGGAGAAAGAAGTAGCTTATTGTTC
CACATATACACACTGTGAGATTCATCCCTCAATGATCCTTGGTGTCTGTGCATCTATTATTCCCTTTCCTGATCATAACCAGTCCCCTAG
AAACACATACCAGTCTGCTATGGGTAAGCAGGCTATGGGAGTTTACATCACCAACTTCCATGTTCGCATGGACACATTGGCCCATGTTCT
CTATTATCCTCAAAAGCCACTTGTGACTACACGGTCTATGGAATATCTACGATTTAGAGAGCTGCCAGCAGGCATCAACTCAATTGTGGC
CATTGCATCATACACTGGATATAATCAGGAAGACTCTGTTATCATGAATCGTTCAGCTGTAGACCGCGGCTTCTTCAGGTCTGTTTTCTA
TCGCTCATACAAAGAACAGGAGTCTAAAAAAGGATTTGATCAAGAAGAAGTTTTTGAGAAGCCTACACGTGAAACATGCCAGGGCATGAG
GCATGCCATTTACGACAAGCTGGATGATGATGGTTTGATAGCTCCAGGGGTTCGTGTATCAGGAGATGATGTTATTATAGGCAAAACAGT
CACCTTGCCTGAAAATGAAGATGAATTGGAGAGCACCAATAGACGCTATACCAAGAGAGACTGTAGCACTTTTCTCAGAACTAGCGAGAC
GGGCATTGTGGATCAGGTTATGGTAACTCTCAATCAGGAAGGATATAAATTTTGTAAAATAAGGGTACGCTCTGTTAGGATTCCACAGAT
TGGAGACAAATTTGCTAGTCGACATGGTCAAAAGGGTACTTGTGGTATTCAGTATAGACAAGAGGATATGCCTTTCACCTGTGAAGGTAT
CACCCCTGATATCATCATCAATCCCCATGCCATCCCCTCTCGTATGACTATTGGTCACTTAATTGAATGCCTTCAAGGGAAGGTATCGGC
TAACAAGGGTGAAATTGGTGATGCCACTCCATTTAATGATGCTGTTAACGTGCAGAAGATTTCTAATCTTTTATCTGATTATGGCTATCA
TCTCAGAGGAAATGAGGTCCTGTACAATGGGTTCACTGGTCGAAAAATCACATCACAAATATTTATTGGCCCCACTTATTACCAGCGTTT
GAAGCATATGGTGGATGATAAGATTCACTCTCGTGCTAGGGGACCTATTCAGATCCTCAATAGACAGCCCATGGAGGGTAGATCTCGTGA
TGGTGGCCTGCGTTTTGGAGAAATGGAACGAGATTGTCAGATTGCCCATGGAGCAGCCCAGTTTTTAAGGGAAAGATTGTTTGAGGCATC
AGATCCATATCAGGTTCATGTTTGCAATCTTTGTGGAATAATGGCGATTGCCAACACCAGGACCCATACATATGAATGCAGGGGCTGCCG
CAATAAAACCCAGATTTCTTTGGTGCGAATGCCTTACGCATGCAAACTATTGTTTCAGGAACTTATGTCTATGAGTATTGCACCGCGAAT
GATGAGTGTTTAGCTATTTTACAGGAGTCAACAAGATAATTAAATATCTTGGTGTCTTGTTTCTATTGTGTGGCTTTTTAAAAATGACAA
ATATGTACTGTGTTGTGATAAAAAGTATTTTATTTGTTTAATGATATGCATGCTTTTCTTCTGTAAATATATAATAAATTTTTGTAGATA

>In-frame_ENST00000257287_ENST00000431623_TCGA-S9-A89Z-01A_CEP135_chr4_56847545_+_POLR2B_chr4_57865783_length(amino acids)=1522AA_start in transcript=124_stop in transcript=4692
MTTAVERKYINIRKRLDQLGYRQTLTVECLPLVEKLFSDLVHTTESLRQSKLSAVKAEKESANFDFVLEPYKLENARLSRENNELYLELM
KLREHSDQHVKELKTSLKKCARETADLKFLNNQYAHKLKLLEKESKAKNERIQQLQEKNLHAVVQTPGGKKRSIAFRRQRMQIDEPVPPS
EVSSYPVPQPDDPYIADLLQVADNRIQELQQEVHQLQEKLAMMESGVRDYSKQIELREREIERLSVALDGGRSPDVLSLESRNKTNEKLI
AHLNIQVDFLQQANKDLEKRIRELMETKETVTSEVVNLSNKNEKLCQELTEIDQLAQQLERHKEEVLETADKELGEAKKEIKRKLSEMQD
LEETMAKLQLELNLCQKEKERLSDELLVKSDLETVVHQLEQEKQRLSKKVESFAVTERQLTLEVERMRLEHGIKRRDRSPSRLDTFLKGI
EEERDYYKKELERLQHIIQRRSCSTSYSAREKSSIFRTPEKGDYNSEIHQITRERDELQRMLERFEKYMEDIQSNVKLLTAERDKLSVLY
NEAQEELSALRKESTQTTAPHNIVSLMEKEKELALSDLRRIMAEKEALREKLEGAKKSAIGQRIVATLPYIKQEVPIIIVFRALGFVSDR
DILEHIIYDFEDPEMMEMVKPSLDEAFVIQEQNVALNFIGSRGAKPGVTKEKRIKYAKEVLQKEMLPHVGVSDFCETKKAYFLGYMVHRL
LLAALGRRELDDRDHYGNKRLDLAGPLLAFLFRGMFKNLLKEVRIYAQKFIDRGKDFNLELAIKTRIISDGLKYSLATGNWGDQKKAHQA
RAGVSQVLNRLTFASTLSHLRRLNSPIGRDGKLAKPRQLHNTLWGMVCPAETPEGHAVGLVKNLALMAYISVGSQPSPILEFLEEWSMEN
LEEISPAAIADATKIFVNGCWVGIHKDPEQLMNTLRKLRRQMDIIVSEVSMIRDIREREIRIYTDAGRICRPLLIVEKQKLLLKKRHIDQ
LKEREYNNYSWQDLVASGVVEYIDTLEEETVMLAMTPDDLQEKEVAYCSTYTHCEIHPSMILGVCASIIPFPDHNQSPRNTYQSAMGKQA
MGVYITNFHVRMDTLAHVLYYPQKPLVTTRSMEYLRFRELPAGINSIVAIASYTGYNQEDSVIMNRSAVDRGFFRSVFYRSYKEQESKKG
FDQEEVFEKPTRETCQGMRHAIYDKLDDDGLIAPGVRVSGDDVIIGKTVTLPENEDELESTNRRYTKRDCSTFLRTSETGIVDQVMVTLN
QEGYKFCKIRVRSVRIPQIGDKFASRHGQKGTCGIQYRQEDMPFTCEGITPDIIINPHAIPSRMTIGHLIECLQGKVSANKGEIGDATPF
NDAVNVQKISNLLSDYGYHLRGNEVLYNGFTGRKITSQIFIGPTYYQRLKHMVDDKIHSRARGPIQILNRQPMEGRSRDGGLRFGEMERD

--------------------------------------------------------------
>In-frame_ENST00000257287_ENST00000441246_TCGA-S9-A89Z-01A_CEP135_chr4_56847545_+_POLR2B_chr4_57865783_length(transcript)=4712nt_BP=1903nt
GACGGAAGGAGCTAGTGGGGGACTCGAGGCCTGAGGGCAATGCGGCTGGAGGCGGAGGCAACGGCGGCTGGAGCTGCCGGACTTTAATTT
TTGGAAGTGAATAAAACTTGTTTTAGAAGACGAGATGACTACAGCTGTAGAGAGAAAGTATATTAATATTAGGAAAAGGCTGGATCAGCT
GGGATACCGCCAGACTCTGACAGTGGAGTGTTTACCTTTGGTAGAAAAACTTTTCAGCGACTTAGTTCATACAACTGAGAGCCTTCGGCA
ATCAAAATTATCTGCTGTGAAAGCTGAAAAAGAAAGTGCCAATTTTGATTTTGTTTTGGAACCCTATAAACTTGAAAATGCAAGATTGAG
TAGAGAAAATAATGAATTATACCTAGAGTTAATGAAACTGAGAGAACATTCAGACCAACACGTTAAAGAGTTGAAAACTTCATTGAAGAA
ATGTGCACGTGAAACAGCTGATCTGAAATTTCTGAATAACCAATATGCTCATAAACTCAAACTGTTGGAGAAAGAGAGCAAAGCTAAGAA
TGAAAGAATTCAACAACTTCAAGAAAAGAATTTGCATGCTGTAGTACAAACTCCAGGTGGCAAGAAAAGAAGTATTGCTTTCAGGCGCCA
GCGTATGCAAATTGATGAACCGGTTCCTCCCTCTGAAGTCAGTTCATATCCAGTTCCTCAACCAGATGACCCTTACATTGCAGACCTCCT
TCAAGTGGCTGATAACAGGATTCAAGAACTTCAACAGGAAGTCCACCAGCTACAAGAAAAGTTAGCAATGATGGAAAGTGGGGTGAGAGA
CTATAGCAAGCAGATTGAGCTAAGAGAACGAGAGATAGAACGACTGTCAGTTGCTTTGGATGGTGGTCGGTCCCCTGATGTCCTTTCTCT
GGAGTCTAGAAATAAAACCAATGAAAAGCTTATTGCTCATTTAAATATTCAGGTTGACTTTCTTCAGCAAGCTAATAAAGACCTGGAGAA
GCGTATACGAGAGCTTATGGAAACCAAGGAAACAGTGACATCTGAAGTCGTTAATTTAAGTAACAAAAATGAAAAACTCTGCCAAGAATT
AACTGAAATAGATCAGTTAGCACAGCAGTTGGAAAGACATAAAGAAGAAGTGCTTGAGACTGCTGATAAAGAGCTTGGGGAAGCAAAGAA
AGAGATTAAAAGAAAGCTCTCTGAAATGCAGGATCTTGAAGAAACAATGGCAAAACTTCAGCTGGAATTGAACTTATGCCAGAAAGAAAA
GGAGAGACTGAGTGATGAACTCCTTGTAAAATCAGACCTAGAAACTGTTGTTCATCAGCTTGAACAAGAAAAGCAAAGACTTAGCAAAAA
AGTTGAAAGTTTTGCAGTTACAGAACGACAACTTACTCTGGAGGTTGAGAGGATGAGACTAGAACATGGAATAAAACGTCGAGACAGGTC
ACCTTCTCGTTTAGATACATTTCTGAAAGGTATAGAAGAAGAACGAGATTATTATAAGAAAGAGCTAGAGAGACTCCAACATATAATACA
GCGAAGATCTTGCTCTACAAGTTATAGCGCACGTGAAAAAAGTTCAATATTTAGAACACCAGAAAAGGGTGATTACAATTCAGAAATTCA
TCAGATCACAAGAGAAAGAGATGAACTTCAGCGTATGCTAGAAAGATTTGAAAAATATATGGAAGATATACAGTCCAATGTTAAATTATT
GACAGCAGAAAGAGATAAACTAAGTGTCTTATATAATGAAGCTCAGGAAGAATTATCTGCCCTAAGAAAGGAATCCACCCAAACCACAGC
ACCCCATAATATTGTTAGTCTTATGGAAAAGGAAAAAGAACTTGCGTTATCTGACTTAAGAAGAATTATGGCAGAAAAGGAAGCTTTAAG
AGAAAAATTAGAGGGTGCCAAGAAGAGTGCTATTGGTCAGCGCATTGTGGCAACTCTACCATATATCAAGCAAGAAGTTCCCATCATTAT
TGTGTTCAGAGCATTAGGTTTTGTGTCCGACAGAGATATTTTAGAACATATTATTTATGATTTTGAAGATCCAGAGATGATGGAAATGGT
TAAACCTTCTCTCGATGAAGCTTTTGTCATCCAAGAACAGAATGTTGCACTAAATTTCATTGGTTCAAGAGGAGCAAAGCCTGGTGTTAC
TAAAGAGAAAAGAATTAAATATGCAAAGGAAGTTTTACAAAAAGAAATGCTCCCTCATGTTGGTGTCAGTGATTTTTGTGAGACCAAAAA
AGCCTATTTCTTGGGATACATGGTTCATAGGTTACTTCTGGCAGCTTTGGGTAGAAGAGAACTAGATGACAGAGATCACTATGGAAACAA
GAGATTGGATCTTGCTGGGCCGCTGCTTGCATTCTTATTTAGAGGTATGTTTAAGAATTTGCTTAAAGAAGTGCGGATCTATGCACAGAA
ATTTATTGATCGAGGAAAGGATTTTAACTTGGAGTTGGCAATTAAAACACGGATCATATCTGATGGCCTAAAATACTCTTTAGCTACTGG
AAACTGGGGTGATCAAAAGAAAGCTCATCAAGCCAGAGCTGGAGTATCTCAGGTGTTAAACCGCCTGACTTTTGCGTCTACTCTTTCTCA
CCTGCGTCGTTTAAATTCTCCTATTGGTAGAGACGGCAAGCTAGCAAAACCAAGACAGTTGCATAATACGTTGTGGGGAATGGTGTGTCC
TGCCGAGACCCCAGAGGGCCATGCTGTAGGACTTGTGAAGAATTTAGCCTTGATGGCGTATATTTCAGTTGGATCTCAACCATCTCCAAT
TCTGGAATTTTTAGAAGAATGGAGTATGGAAAATTTAGAAGAAATTTCTCCTGCAGCTATTGCTGATGCAACCAAGATTTTTGTTAATGG
CTGCTGGGTTGGAATACATAAAGATCCCGAACAACTTATGAACACCCTAAGGAAATTGAGACGTCAGATGGACATCATTGTGTCTGAAGT
TTCTATGATCAGAGATATTCGAGAGAGGGAGATTCGGATCTATACGGATGCAGGCCGTATTTGTAGACCACTTCTGATTGTGGAAAAACA
AAAGCTACTTTTGAAGAAGAGGCATATTGACCAATTGAAAGAGAGAGAATATAACAACTATAGTTGGCAGGATCTTGTGGCCAGTGGGGT
AGTGGAGTATATTGATACCCTGGAAGAAGAAACAGTGATGCTTGCAATGACTCCAGATGATTTACAGGAGAAAGAAGTAGCTTATTGTTC
CACATATACACACTGTGAGATTCATCCCTCAATGATCCTTGGTGTCTGTGCATCTATTATTCCCTTTCCTGATCATAACCAGTCCCCTAG
AAACACATACCAGTCTGCTATGGGTAAGCAGGCTATGGGAGTTTACATCACCAACTTCCATGTTCGCATGGACACATTGGCCCATGTTCT
CTATTATCCTCAAAAGCCACTTGTGACTACACGGTCTATGGAATATCTACGATTTAGAGAGCTGCCAGCAGGCATCAACTCAATTGTGGC
CATTGCATCATACACTGGATATAATCAGGAAGACTCTGTTATCATGAATCGTTCAGCTGTAGACCGCGGCTTCTTCAGGTCTGTTTTCTA
TCGCTCATACAAAGAACAGGAGTCTAAAAAAGGATTTGATCAAGAAGAAGTTTTTGAGAAGCCTACACGTGAAACATGCCAGGGCATGAG
GCATGCCATTTACGACAAGCTGGATGATGATGGTTTGATAGCTCCAGGGGTTCGTGTATCAGGAGATGATGTTATTATAGGCAAAACAGT
CACCTTGCCTGAAAATGAAGATGAATTGGAGAGCACCAATAGACGCTATACCAAGAGAGACTGTAGCACTTTTCTCAGAACTAGCGAGAC
GGGCATTGTGGATCAGGTTATGGTAACTCTCAATCAGGAAGGATATAAATTTTGTAAAATAAGGGTACGCTCTGTTAGGATTCCACAGAT
TGGAGACAAATTTGCTAGTCGACATGGTCAAAAGGGTACTTGTGGTATTCAGTATAGACAAGAGGATATGCCTTTCACCTGTGAAGGTAT
CACCCCTGATATCATCATCAATCCCCATGCCATCCCCTCTCGTATGACTATTGGTCACTTAATTGAATGCCTTCAAGGGAAGGTATCGGC
TAACAAGGGTGAAATTGGTGATGCCACTCCATTTAATGATGCTGTTAACGTGCAGAAGATTTCTAATCTTTTATCTGATTATGGCTATCA
TCTCAGAGGAAATGAGGTCCTGTACAATGGGTTCACTGGTCGAAAAATCACATCACAAATATTTATTGGCCCCACTTATTACCAGCGTTT
GAAGCATATGGTGGATGATAAGATTCACTCTCGTGCTAGGGGACCTATTCAGATCCTCAATAGACAGCCCATGGAGGGTAGATCTCGTGA
TGGTGGCCTGCGTTTTGGAGAAATGGAACGAGATTGTCAGATTGCCCATGGAGCAGCCCAGTTTTTAAGGGAAAGATTGTTTGAGGCATC
AGATCCATATCAGGTTCATGTTTGCAATCTTTGTGGAATAATGGCGATTGCCAACACCAGGACCCATACATATGAATGCAGGGGCTGCCG
CAATAAAACCCAGATTTCTTTGGTGCGAATGCCTTACGCATGCAAACTATTGTTTCAGGAACTTATGTCTATGAGTATTGCACCGCGAAT

>In-frame_ENST00000257287_ENST00000441246_TCGA-S9-A89Z-01A_CEP135_chr4_56847545_+_POLR2B_chr4_57865783_length(amino acids)=1522AA_start in transcript=124_stop in transcript=4692
MTTAVERKYINIRKRLDQLGYRQTLTVECLPLVEKLFSDLVHTTESLRQSKLSAVKAEKESANFDFVLEPYKLENARLSRENNELYLELM
KLREHSDQHVKELKTSLKKCARETADLKFLNNQYAHKLKLLEKESKAKNERIQQLQEKNLHAVVQTPGGKKRSIAFRRQRMQIDEPVPPS
EVSSYPVPQPDDPYIADLLQVADNRIQELQQEVHQLQEKLAMMESGVRDYSKQIELREREIERLSVALDGGRSPDVLSLESRNKTNEKLI
AHLNIQVDFLQQANKDLEKRIRELMETKETVTSEVVNLSNKNEKLCQELTEIDQLAQQLERHKEEVLETADKELGEAKKEIKRKLSEMQD
LEETMAKLQLELNLCQKEKERLSDELLVKSDLETVVHQLEQEKQRLSKKVESFAVTERQLTLEVERMRLEHGIKRRDRSPSRLDTFLKGI
EEERDYYKKELERLQHIIQRRSCSTSYSAREKSSIFRTPEKGDYNSEIHQITRERDELQRMLERFEKYMEDIQSNVKLLTAERDKLSVLY
NEAQEELSALRKESTQTTAPHNIVSLMEKEKELALSDLRRIMAEKEALREKLEGAKKSAIGQRIVATLPYIKQEVPIIIVFRALGFVSDR
DILEHIIYDFEDPEMMEMVKPSLDEAFVIQEQNVALNFIGSRGAKPGVTKEKRIKYAKEVLQKEMLPHVGVSDFCETKKAYFLGYMVHRL
LLAALGRRELDDRDHYGNKRLDLAGPLLAFLFRGMFKNLLKEVRIYAQKFIDRGKDFNLELAIKTRIISDGLKYSLATGNWGDQKKAHQA
RAGVSQVLNRLTFASTLSHLRRLNSPIGRDGKLAKPRQLHNTLWGMVCPAETPEGHAVGLVKNLALMAYISVGSQPSPILEFLEEWSMEN
LEEISPAAIADATKIFVNGCWVGIHKDPEQLMNTLRKLRRQMDIIVSEVSMIRDIREREIRIYTDAGRICRPLLIVEKQKLLLKKRHIDQ
LKEREYNNYSWQDLVASGVVEYIDTLEEETVMLAMTPDDLQEKEVAYCSTYTHCEIHPSMILGVCASIIPFPDHNQSPRNTYQSAMGKQA
MGVYITNFHVRMDTLAHVLYYPQKPLVTTRSMEYLRFRELPAGINSIVAIASYTGYNQEDSVIMNRSAVDRGFFRSVFYRSYKEQESKKG
FDQEEVFEKPTRETCQGMRHAIYDKLDDDGLIAPGVRVSGDDVIIGKTVTLPENEDELESTNRRYTKRDCSTFLRTSETGIVDQVMVTLN
QEGYKFCKIRVRSVRIPQIGDKFASRHGQKGTCGIQYRQEDMPFTCEGITPDIIINPHAIPSRMTIGHLIECLQGKVSANKGEIGDATPF
NDAVNVQKISNLLSDYGYHLRGNEVLYNGFTGRKITSQIFIGPTYYQRLKHMVDDKIHSRARGPIQILNRQPMEGRSRDGGLRFGEMERD

--------------------------------------------------------------
>In-frame_ENST00000257287_ENST00000314595_TCGA-S9-A89Z-01A_CEP135_chr4_56847545_+_POLR2B_chr4_57865783_length(transcript)=4873nt_BP=1903nt
GACGGAAGGAGCTAGTGGGGGACTCGAGGCCTGAGGGCAATGCGGCTGGAGGCGGAGGCAACGGCGGCTGGAGCTGCCGGACTTTAATTT
TTGGAAGTGAATAAAACTTGTTTTAGAAGACGAGATGACTACAGCTGTAGAGAGAAAGTATATTAATATTAGGAAAAGGCTGGATCAGCT
GGGATACCGCCAGACTCTGACAGTGGAGTGTTTACCTTTGGTAGAAAAACTTTTCAGCGACTTAGTTCATACAACTGAGAGCCTTCGGCA
ATCAAAATTATCTGCTGTGAAAGCTGAAAAAGAAAGTGCCAATTTTGATTTTGTTTTGGAACCCTATAAACTTGAAAATGCAAGATTGAG
TAGAGAAAATAATGAATTATACCTAGAGTTAATGAAACTGAGAGAACATTCAGACCAACACGTTAAAGAGTTGAAAACTTCATTGAAGAA
ATGTGCACGTGAAACAGCTGATCTGAAATTTCTGAATAACCAATATGCTCATAAACTCAAACTGTTGGAGAAAGAGAGCAAAGCTAAGAA
TGAAAGAATTCAACAACTTCAAGAAAAGAATTTGCATGCTGTAGTACAAACTCCAGGTGGCAAGAAAAGAAGTATTGCTTTCAGGCGCCA
GCGTATGCAAATTGATGAACCGGTTCCTCCCTCTGAAGTCAGTTCATATCCAGTTCCTCAACCAGATGACCCTTACATTGCAGACCTCCT
TCAAGTGGCTGATAACAGGATTCAAGAACTTCAACAGGAAGTCCACCAGCTACAAGAAAAGTTAGCAATGATGGAAAGTGGGGTGAGAGA
CTATAGCAAGCAGATTGAGCTAAGAGAACGAGAGATAGAACGACTGTCAGTTGCTTTGGATGGTGGTCGGTCCCCTGATGTCCTTTCTCT
GGAGTCTAGAAATAAAACCAATGAAAAGCTTATTGCTCATTTAAATATTCAGGTTGACTTTCTTCAGCAAGCTAATAAAGACCTGGAGAA
GCGTATACGAGAGCTTATGGAAACCAAGGAAACAGTGACATCTGAAGTCGTTAATTTAAGTAACAAAAATGAAAAACTCTGCCAAGAATT
AACTGAAATAGATCAGTTAGCACAGCAGTTGGAAAGACATAAAGAAGAAGTGCTTGAGACTGCTGATAAAGAGCTTGGGGAAGCAAAGAA
AGAGATTAAAAGAAAGCTCTCTGAAATGCAGGATCTTGAAGAAACAATGGCAAAACTTCAGCTGGAATTGAACTTATGCCAGAAAGAAAA
GGAGAGACTGAGTGATGAACTCCTTGTAAAATCAGACCTAGAAACTGTTGTTCATCAGCTTGAACAAGAAAAGCAAAGACTTAGCAAAAA
AGTTGAAAGTTTTGCAGTTACAGAACGACAACTTACTCTGGAGGTTGAGAGGATGAGACTAGAACATGGAATAAAACGTCGAGACAGGTC
ACCTTCTCGTTTAGATACATTTCTGAAAGGTATAGAAGAAGAACGAGATTATTATAAGAAAGAGCTAGAGAGACTCCAACATATAATACA
GCGAAGATCTTGCTCTACAAGTTATAGCGCACGTGAAAAAAGTTCAATATTTAGAACACCAGAAAAGGGTGATTACAATTCAGAAATTCA
TCAGATCACAAGAGAAAGAGATGAACTTCAGCGTATGCTAGAAAGATTTGAAAAATATATGGAAGATATACAGTCCAATGTTAAATTATT
GACAGCAGAAAGAGATAAACTAAGTGTCTTATATAATGAAGCTCAGGAAGAATTATCTGCCCTAAGAAAGGAATCCACCCAAACCACAGC
ACCCCATAATATTGTTAGTCTTATGGAAAAGGAAAAAGAACTTGCGTTATCTGACTTAAGAAGAATTATGGCAGAAAAGGAAGCTTTAAG
AGAAAAATTAGAGGGTGCCAAGAAGAGTGCTATTGGTCAGCGCATTGTGGCAACTCTACCATATATCAAGCAAGAAGTTCCCATCATTAT
TGTGTTCAGAGCATTAGGTTTTGTGTCCGACAGAGATATTTTAGAACATATTATTTATGATTTTGAAGATCCAGAGATGATGGAAATGGT
TAAACCTTCTCTCGATGAAGCTTTTGTCATCCAAGAACAGAATGTTGCACTAAATTTCATTGGTTCAAGAGGAGCAAAGCCTGGTGTTAC
TAAAGAGAAAAGAATTAAATATGCAAAGGAAGTTTTACAAAAAGAAATGCTCCCTCATGTTGGTGTCAGTGATTTTTGTGAGACCAAAAA
AGCCTATTTCTTGGGATACATGGTTCATAGGTTACTTCTGGCAGCTTTGGGTAGAAGAGAACTAGATGACAGAGATCACTATGGAAACAA
GAGATTGGATCTTGCTGGGCCGCTGCTTGCATTCTTATTTAGAGGTATGTTTAAGAATTTGCTTAAAGAAGTGCGGATCTATGCACAGAA
ATTTATTGATCGAGGAAAGGATTTTAACTTGGAGTTGGCAATTAAAACACGGATCATATCTGATGGCCTAAAATACTCTTTAGCTACTGG
AAACTGGGGTGATCAAAAGAAAGCTCATCAAGCCAGAGCTGGAGTATCTCAGGTGTTAAACCGCCTGACTTTTGCGTCTACTCTTTCTCA
CCTGCGTCGTTTAAATTCTCCTATTGGTAGAGACGGCAAGCTAGCAAAACCAAGACAGTTGCATAATACGTTGTGGGGAATGGTGTGTCC
TGCCGAGACCCCAGAGGGCCATGCTGTAGGACTTGTGAAGAATTTAGCCTTGATGGCGTATATTTCAGTTGGATCTCAACCATCTCCAAT
TCTGGAATTTTTAGAAGAATGGAGTATGGAAAATTTAGAAGAAATTTCTCCTGCAGCTATTGCTGATGCAACCAAGATTTTTGTTAATGG
CTGCTGGGTTGGAATACATAAAGATCCCGAACAACTTATGAACACCCTAAGGAAATTGAGACGTCAGATGGACATCATTGTGTCTGAAGT
TTCTATGATCAGAGATATTCGAGAGAGGGAGATTCGGATCTATACGGATGCAGGCCGTATTTGTAGACCACTTCTGATTGTGGAAAAACA
AAAGCTACTTTTGAAGAAGAGGCATATTGACCAATTGAAAGAGAGAGAATATAACAACTATAGTTGGCAGGATCTTGTGGCCAGTGGGGT
AGTGGAGTATATTGATACCCTGGAAGAAGAAACAGTGATGCTTGCAATGACTCCAGATGATTTACAGGAGAAAGAAGTAGCTTATTGTTC
CACATATACACACTGTGAGATTCATCCCTCAATGATCCTTGGTGTCTGTGCATCTATTATTCCCTTTCCTGATCATAACCAGTCCCCTAG
AAACACATACCAGTCTGCTATGGGTAAGCAGGCTATGGGAGTTTACATCACCAACTTCCATGTTCGCATGGACACATTGGCCCATGTTCT
CTATTATCCTCAAAAGCCACTTGTGACTACACGGTCTATGGAATATCTACGATTTAGAGAGCTGCCAGCAGGCATCAACTCAATTGTGGC
CATTGCATCATACACTGGATATAATCAGGAAGACTCTGTTATCATGAATCGTTCAGCTGTAGACCGCGGCTTCTTCAGGTCTGTTTTCTA
TCGCTCATACAAAGAACAGGAGTCTAAAAAAGGATTTGATCAAGAAGAAGTTTTTGAGAAGCCTACACGTGAAACATGCCAGGGCATGAG
GCATGCCATTTACGACAAGCTGGATGATGATGGTTTGATAGCTCCAGGGGTTCGTGTATCAGGAGATGATGTTATTATAGGCAAAACAGT
CACCTTGCCTGAAAATGAAGATGAATTGGAGAGCACCAATAGACGCTATACCAAGAGAGACTGTAGCACTTTTCTCAGAACTAGCGAGAC
GGGCATTGTGGATCAGGTTATGGTAACTCTCAATCAGGAAGGATATAAATTTTGTAAAATAAGGGTACGCTCTGTTAGGATTCCACAGAT
TGGAGACAAATTTGCTAGTCGACATGGTCAAAAGGGTACTTGTGGTATTCAGTATAGACAAGAGGATATGCCTTTCACCTGTGAAGGTAT
CACCCCTGATATCATCATCAATCCCCATGCCATCCCCTCTCGTATGACTATTGGTCACTTAATTGAATGCCTTCAAGGGAAGGTATCGGC
TAACAAGGGTGAAATTGGTGATGCCACTCCATTTAATGATGCTGTTAACGTGCAGAAGATTTCTAATCTTTTATCTGATTATGGCTATCA
TCTCAGAGGAAATGAGGTCCTGTACAATGGGTTCACTGGTCGAAAAATCACATCACAAATATTTATTGGCCCCACTTATTACCAGCGTTT
GAAGCATATGGTGGATGATAAGATTCACTCTCGTGCTAGGGGACCTATTCAGATCCTCAATAGACAGCCCATGGAGGGTAGATCTCGTGA
TGGTGGCCTGCGTTTTGGAGAAATGGAACGAGATTGTCAGATTGCCCATGGAGCAGCCCAGTTTTTAAGGGAAAGATTGTTTGAGGCATC
AGATCCATATCAGGTTCATGTTTGCAATCTTTGTGGAATAATGGCGATTGCCAACACCAGGACCCATACATATGAATGCAGGGGCTGCCG
CAATAAAACCCAGATTTCTTTGGTGCGAATGCCTTACGCATGCAAACTATTGTTTCAGGAACTTATGTCTATGAGTATTGCACCGCGAAT
GATGAGTGTTTAGCTATTTTACAGGAGTCAACAAGATAATTAAATATCTTGGTGTCTTGTTTCTATTGTGTGGCTTTTTAAAAATGACAA
ATATGTACTGTGTTGTGATAAAAAGTATTTTATTTGTTTAATGATATGCATGCTTTTCTTCTGTAAATATATAATAAATTTTTGTAGATA

>In-frame_ENST00000257287_ENST00000314595_TCGA-S9-A89Z-01A_CEP135_chr4_56847545_+_POLR2B_chr4_57865783_length(amino acids)=1522AA_start in transcript=124_stop in transcript=4692
MTTAVERKYINIRKRLDQLGYRQTLTVECLPLVEKLFSDLVHTTESLRQSKLSAVKAEKESANFDFVLEPYKLENARLSRENNELYLELM
KLREHSDQHVKELKTSLKKCARETADLKFLNNQYAHKLKLLEKESKAKNERIQQLQEKNLHAVVQTPGGKKRSIAFRRQRMQIDEPVPPS
EVSSYPVPQPDDPYIADLLQVADNRIQELQQEVHQLQEKLAMMESGVRDYSKQIELREREIERLSVALDGGRSPDVLSLESRNKTNEKLI
AHLNIQVDFLQQANKDLEKRIRELMETKETVTSEVVNLSNKNEKLCQELTEIDQLAQQLERHKEEVLETADKELGEAKKEIKRKLSEMQD
LEETMAKLQLELNLCQKEKERLSDELLVKSDLETVVHQLEQEKQRLSKKVESFAVTERQLTLEVERMRLEHGIKRRDRSPSRLDTFLKGI
EEERDYYKKELERLQHIIQRRSCSTSYSAREKSSIFRTPEKGDYNSEIHQITRERDELQRMLERFEKYMEDIQSNVKLLTAERDKLSVLY
NEAQEELSALRKESTQTTAPHNIVSLMEKEKELALSDLRRIMAEKEALREKLEGAKKSAIGQRIVATLPYIKQEVPIIIVFRALGFVSDR
DILEHIIYDFEDPEMMEMVKPSLDEAFVIQEQNVALNFIGSRGAKPGVTKEKRIKYAKEVLQKEMLPHVGVSDFCETKKAYFLGYMVHRL
LLAALGRRELDDRDHYGNKRLDLAGPLLAFLFRGMFKNLLKEVRIYAQKFIDRGKDFNLELAIKTRIISDGLKYSLATGNWGDQKKAHQA
RAGVSQVLNRLTFASTLSHLRRLNSPIGRDGKLAKPRQLHNTLWGMVCPAETPEGHAVGLVKNLALMAYISVGSQPSPILEFLEEWSMEN
LEEISPAAIADATKIFVNGCWVGIHKDPEQLMNTLRKLRRQMDIIVSEVSMIRDIREREIRIYTDAGRICRPLLIVEKQKLLLKKRHIDQ
LKEREYNNYSWQDLVASGVVEYIDTLEEETVMLAMTPDDLQEKEVAYCSTYTHCEIHPSMILGVCASIIPFPDHNQSPRNTYQSAMGKQA
MGVYITNFHVRMDTLAHVLYYPQKPLVTTRSMEYLRFRELPAGINSIVAIASYTGYNQEDSVIMNRSAVDRGFFRSVFYRSYKEQESKKG
FDQEEVFEKPTRETCQGMRHAIYDKLDDDGLIAPGVRVSGDDVIIGKTVTLPENEDELESTNRRYTKRDCSTFLRTSETGIVDQVMVTLN
QEGYKFCKIRVRSVRIPQIGDKFASRHGQKGTCGIQYRQEDMPFTCEGITPDIIINPHAIPSRMTIGHLIECLQGKVSANKGEIGDATPF
NDAVNVQKISNLLSDYGYHLRGNEVLYNGFTGRKITSQIFIGPTYYQRLKHMVDDKIHSRARGPIQILNRQPMEGRSRDGGLRFGEMERD

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Fusion Gene PPI Analysis for CEP135-POLR2B


check button Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in

ChiPPI page.


check button Protein-protein interactors with each fusion partner protein in wild-type (BIOGRID-3.4.160)
HgeneHgene's interactorsTgeneTgene's interactors


check button - Retained PPIs in in-frame fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenStill interaction with


check button - Lost PPIs in in-frame fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenInteraction lost with


check button - Retained PPIs, but lost function due to frame-shift fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenInteraction lost with


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Related Drugs for CEP135-POLR2B


check button Drugs targeting genes involved in this fusion gene.
(DrugBank Version 5.1.8 2021-05-08)
PartnerGeneUniProtAccDrugBank IDDrug nameDrug activityDrug typeDrug status

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Related Diseases for CEP135-POLR2B


check button Diseases associated with fusion partners.
(DisGeNet 4.0)
PartnerGeneDisease IDDisease name# pubmedsSource
HgeneCEP135C3553414MICROCEPHALY 8, PRIMARY, AUTOSOMAL RECESSIVE1CTD_human;GENOMICS_ENGLAND