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Center for Computational Systems Medicine
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Fusion Gene Summary

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Fusion Gene ORF analysis

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Fusion Genomic Features

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Fusion Protein Features

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Fusion Gene Sequence

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Fusion Gene PPI analysis

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Related Drugs

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Related Diseases

Fusion gene:CNOT8-PROL1 (FusionGDB2 ID:17815)

Fusion Gene Summary for CNOT8-PROL1

check button Fusion gene summary
Fusion gene informationFusion gene name: CNOT8-PROL1
Fusion gene ID: 17815
HgeneTgene
Gene symbol

CNOT8

PROL1

Gene ID

9337

58503

Gene nameCCR4-NOT transcription complex subunit 8opiorphin prepropeptide
SynonymsCAF1|CALIF|Caf1b|POP2|hCAF1BPLP|PRL1|PROL1|opiorphin
Cytomap

5q33.2

4q13.3

Type of geneprotein-codingprotein-coding
DescriptionCCR4-NOT transcription complex subunit 8CAF1-like proteinCAF2CALIFpCCR4-associated factor 8PGK promoter directed over productionopiorphin prepropeptidebasic proline-rich lacrimal proteinbasic proline-rich proteinproline rich, lacrimal 1proline-rich 1proline-rich protein 1
Modification date2020031320200313
UniProtAcc

Q9UFF9

.
Ensembl transtripts involved in fusion geneENST00000523698, ENST00000517876, 
ENST00000521450, ENST00000403027, 
ENST00000524105, ENST00000285896, 
ENST00000520671, ENST00000521583, 
ENST00000519404, ENST00000521402, 
ENST00000399575, ENST00000514338, 
Fusion gene scores* DoF score11 X 9 X 5=4951 X 2 X 1=2
# samples 101
** MAII scorelog2(10/495*10)=-2.30742852519225
possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs).
DoF>8 and MAII<0		log2(1/2*10)=2.32192809488736
Context

PubMed: CNOT8 [Title/Abstract] AND PROL1 [Title/Abstract] AND fusion [Title/Abstract]

Most frequent breakpointCNOT8(154244945)-PROL1(71275097), # samples:2
Anticipated loss of major functional domain due to fusion event.CNOT8-PROL1 seems lost the major protein functional domain in Hgene partner, which is a cell metabolism gene due to the frame-shifted ORF.
CNOT8-PROL1 seems lost the major protein functional domain in Tgene partner, which is a essential gene due to the frame-shifted ORF.
* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

check button Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez
PartnerGeneGO IDGO termPubMed ID
HgeneCNOT8

GO:0043928

exonucleolytic nuclear-transcribed mRNA catabolic process involved in deadenylation-dependent decay

20065043

TgenePROL1

GO:0051930

regulation of sensory perception of pain

17101991


check buttonFusion gene breakpoints across CNOT8 (5'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

check buttonFusion gene breakpoints across PROL1 (3'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

check button Fusion gene information from two resources (ChiTars 5.0 and ChimerDB 4.0)
* All genome coordinats were lifted-over on hg19.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.
SourceDiseaseSampleHgeneHchrHbpHstrandTgeneTchrTbpTstrand
ChimerDB4UCSTCGA-NA-A4QWCNOT8chr5

154244945

+PROL1chr4

71275096

+
ChimerDB4UCSTCGA-NA-A4QWCNOT8chr5

154244945

+PROL1chr4

71275097

+
ChimerDB4UCSTCGA-NA-A4QW-01ACNOT8chr5

154244945

-PROL1chr4

71275097

+


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Fusion Gene ORF analysis for CNOT8-PROL1

check button Open reading frame (ORF) analsis of fusion genes based on Ensembl gene isoform structure.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.
ORFHenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrand
intron-3CDSENST00000523698ENST00000399575CNOT8chr5

154244945

+PROL1chr4

71275096

+
intron-3UTRENST00000523698ENST00000514338CNOT8chr5

154244945

+PROL1chr4

71275096

+
Frame-shiftENST00000517876ENST00000399575CNOT8chr5

154244945

+PROL1chr4

71275096

+
5CDS-3UTRENST00000517876ENST00000514338CNOT8chr5

154244945

+PROL1chr4

71275096

+
intron-3CDSENST00000521450ENST00000399575CNOT8chr5

154244945

+PROL1chr4

71275096

+
intron-3UTRENST00000521450ENST00000514338CNOT8chr5

154244945

+PROL1chr4

71275096

+
Frame-shiftENST00000403027ENST00000399575CNOT8chr5

154244945

+PROL1chr4

71275096

+
5CDS-3UTRENST00000403027ENST00000514338CNOT8chr5

154244945

+PROL1chr4

71275096

+
5UTR-3CDSENST00000524105ENST00000399575CNOT8chr5

154244945

+PROL1chr4

71275096

+
5UTR-3UTRENST00000524105ENST00000514338CNOT8chr5

154244945

+PROL1chr4

71275096

+
Frame-shiftENST00000285896ENST00000399575CNOT8chr5

154244945

+PROL1chr4

71275096

+
5CDS-3UTRENST00000285896ENST00000514338CNOT8chr5

154244945

+PROL1chr4

71275096

+
5UTR-3CDSENST00000520671ENST00000399575CNOT8chr5

154244945

+PROL1chr4

71275096

+
5UTR-3UTRENST00000520671ENST00000514338CNOT8chr5

154244945

+PROL1chr4

71275096

+
5UTR-3CDSENST00000521583ENST00000399575CNOT8chr5

154244945

+PROL1chr4

71275096

+
5UTR-3UTRENST00000521583ENST00000514338CNOT8chr5

154244945

+PROL1chr4

71275096

+
Frame-shiftENST00000519404ENST00000399575CNOT8chr5

154244945

+PROL1chr4

71275096

+
5CDS-3UTRENST00000519404ENST00000514338CNOT8chr5

154244945

+PROL1chr4

71275096

+
3UTR-3CDSENST00000521402ENST00000399575CNOT8chr5

154244945

+PROL1chr4

71275096

+
3UTR-3UTRENST00000521402ENST00000514338CNOT8chr5

154244945

+PROL1chr4

71275096

+
intron-3CDSENST00000523698ENST00000399575CNOT8chr5

154244945

+PROL1chr4

71275097

+
intron-3UTRENST00000523698ENST00000514338CNOT8chr5

154244945

+PROL1chr4

71275097

+
Frame-shiftENST00000517876ENST00000399575CNOT8chr5

154244945

+PROL1chr4

71275097

+
5CDS-3UTRENST00000517876ENST00000514338CNOT8chr5

154244945

+PROL1chr4

71275097

+
intron-3CDSENST00000521450ENST00000399575CNOT8chr5

154244945

+PROL1chr4

71275097

+
intron-3UTRENST00000521450ENST00000514338CNOT8chr5

154244945

+PROL1chr4

71275097

+
Frame-shiftENST00000403027ENST00000399575CNOT8chr5

154244945

+PROL1chr4

71275097

+
5CDS-3UTRENST00000403027ENST00000514338CNOT8chr5

154244945

+PROL1chr4

71275097

+
5UTR-3CDSENST00000524105ENST00000399575CNOT8chr5

154244945

+PROL1chr4

71275097

+
5UTR-3UTRENST00000524105ENST00000514338CNOT8chr5

154244945

+PROL1chr4

71275097

+
Frame-shiftENST00000285896ENST00000399575CNOT8chr5

154244945

+PROL1chr4

71275097

+
5CDS-3UTRENST00000285896ENST00000514338CNOT8chr5

154244945

+PROL1chr4

71275097

+
5UTR-3CDSENST00000520671ENST00000399575CNOT8chr5

154244945

+PROL1chr4

71275097

+
5UTR-3UTRENST00000520671ENST00000514338CNOT8chr5

154244945

+PROL1chr4

71275097

+
5UTR-3CDSENST00000521583ENST00000399575CNOT8chr5

154244945

+PROL1chr4

71275097

+
5UTR-3UTRENST00000521583ENST00000514338CNOT8chr5

154244945

+PROL1chr4

71275097

+
Frame-shiftENST00000519404ENST00000399575CNOT8chr5

154244945

+PROL1chr4

71275097

+
5CDS-3UTRENST00000519404ENST00000514338CNOT8chr5

154244945

+PROL1chr4

71275097

+
3UTR-3CDSENST00000521402ENST00000399575CNOT8chr5

154244945

+PROL1chr4

71275097

+
3UTR-3UTRENST00000521402ENST00000514338CNOT8chr5

154244945

+PROL1chr4

71275097

+

check buttonORFfinder result based on the fusion transcript sequence of in-frame fusion genes.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandSeq length
(transcript)		BP loci
(transcript)		Predicted start
(transcript)		Predicted stop
(transcript)		Seq length
(amino acids)

check buttonDeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandNo-coding scoreCoding score

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Fusion Genomic Features for CNOT8-PROL1


check buttonFusionAI prediction of the potential fusion gene breakpoint based on the pre-mature RNA sequence context (+/- 5kb of individual partner genes, total 20kb length sequence). FusionAI is a fusion gene breakpoint classifier based on convolutional neural network by comparing the fusion positive and negative sequence context of ~ 20K fusion gene data. From here, we can have the relative potentency of the 20K genomic sequence how individual sequnce will be likely used as the gene fusion breakpoints.
HgeneHchrHbpHstrandTgeneTchrTbpTstrand1-pp (fusion gene breakpoint)
CNOT8chr5154244945+PROL1chr471275096+2.95E-070.99999976
CNOT8chr5154244945+PROL1chr471275096+2.95E-070.99999976

check buttonDistribution of 44 human genomic features loci across 20kb length fusion breakpoint regions. We integrated a total of 44 different types of human genomic feature loci information across five big categories including virus integration sites, repeats, structural variants, chromatin states, and gene expression regulation. More details are in help page.

check buttonDistribution of 44 human genomic features loci across 20kb length fusion breakpoint regions that are ovelapped with the top 1% feature importance score regions. More details are in help page.

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Fusion Protein Features for CNOT8-PROL1


check button Go to

FGviewer for the breakpoints of :-:

.
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
FGviewer

check buttonMain function of each fusion partner protein. (from UniProt)
HgeneTgene
CNOT8

Q9UFF9

.
FUNCTION: Has 3'-5' poly(A) exoribonuclease activity for synthetic poly(A) RNA substrate. Its function seems to be partially redundant with that of CNOT7. Catalytic component of the CCR4-NOT complex which is linked to various cellular processes including bulk mRNA degradation, miRNA-mediated repression, translational repression during translational initiation and general transcription regulation. During miRNA-mediated repression the complex seems also to act as translational repressor during translational initiation. Additional complex functions may be a consequence of its influence on mRNA expression. Associates with members of the BTG family such as TOB1 and BTG2 and is required for their anti-proliferative activity. {ECO:0000269|PubMed:12771185, ECO:0000269|PubMed:19605561, ECO:0000269|PubMed:20065043, ECO:0000269|PubMed:23236473}.FUNCTION: Might normally function as a transcriptional repressor. EWS-fusion-proteins (EFPS) may play a role in the tumorigenic process. They may disturb gene expression by mimicking, or interfering with the normal function of CTD-POLII within the transcription initiation complex. They may also contribute to an aberrant activation of the fusion protein target genes.

check buttonRetention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at

download page


* Minus value of BPloci means that the break pointn is located before the CDS.
- In-frame and retained protein feature among the 13 regional features.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note

- In-frame and not-retained protein feature among the 13 regional features.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note


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Fusion Gene Sequence for CNOT8-PROL1


check button For in-frame fusion transcripts, we provide the fusion transcript sequences and fusion amino acid sequences. To have fusion amino acid sequence, we ran ORFfinder and chose the longest ORF among the all predicted ones.

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Fusion Gene PPI Analysis for CNOT8-PROL1


check button Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in

ChiPPI page.


check button Protein-protein interactors with each fusion partner protein in wild-type (BIOGRID-3.4.160)
HgeneHgene's interactorsTgeneTgene's interactors


check button - Retained PPIs in in-frame fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenStill interaction with


check button - Lost PPIs in in-frame fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenInteraction lost with


check button - Retained PPIs, but lost function due to frame-shift fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenInteraction lost with


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Related Drugs for CNOT8-PROL1


check button Drugs targeting genes involved in this fusion gene.
(DrugBank Version 5.1.8 2021-05-08)
PartnerGeneUniProtAccDrugBank IDDrug nameDrug activityDrug typeDrug status

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Related Diseases for CNOT8-PROL1


check button Diseases associated with fusion partners.
(DisGeNet 4.0)
PartnerGeneDisease IDDisease name# pubmedsSource