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Center for Computational Systems Medicine
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Fusion Gene Summary

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Fusion Gene ORF analysis

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Fusion Genomic Features

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Fusion Protein Features

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Fusion Gene Sequence

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Fusion Gene PPI analysis

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Related Drugs

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Related Diseases

Fusion gene:CYP4B1-CYP4X1 (FusionGDB2 ID:21078)

Fusion Gene Summary for CYP4B1-CYP4X1

check button Fusion gene summary
Fusion gene informationFusion gene name: CYP4B1-CYP4X1
Fusion gene ID: 21078
HgeneTgene
Gene symbol

CYP4B1

CYP4X1

Gene ID

1580

260293

Gene namecytochrome P450 family 4 subfamily B member 1cytochrome P450 family 4 subfamily X member 1
SynonymsCYPIVB1|P-450HPCYPIVX1
Cytomap

1p33

1p33|1

Type of geneprotein-codingprotein-coding
Descriptioncytochrome P450 4B1cytochrome P450, family 4, subfamily B, polypeptide 1cytochrome P450, subfamily IVB, polypeptide 1cytochrome P450-HPmicrosomal monooxygenasecytochrome P450 4X1cytochrome P450, family 4, subfamily X, polypeptide 1
Modification date2020031320200313
UniProtAcc.

Q8N118

Ensembl transtripts involved in fusion geneENST00000546128, ENST00000371923, 
ENST00000271153, ENST00000371919, 
ENST00000452782, 		ENST00000538609, 
ENST00000371901, ENST00000466294, 
Fusion gene scores* DoF score6 X 5 X 4=1204 X 4 X 3=48
# samples 75
** MAII scorelog2(7/120*10)=-0.777607578663552
possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs).
DoF>8 and MAII<0		log2(5/48*10)=0.0588936890535686
effective Gene in Pan-Cancer Fusion Genes (eGinPCFGs).
DoF>8 and MAII>0
Context

PubMed: CYP4B1 [Title/Abstract] AND CYP4X1 [Title/Abstract] AND fusion [Title/Abstract]

A chimeric fusion protein of cytochrome CYP4B1 and green fluorescent protein for detection of pro-drug activating gene delivery and for gene therapy in malignant glioma (pmid: 10026902)
Most frequent breakpointCYP4B1(47280936)-CYP4X1(47495661), # samples:6
Anticipated loss of major functional domain due to fusion event.CYP4B1-CYP4X1 seems lost the major protein functional domain in Hgene partner, which is a IUPHAR drug target due to the frame-shifted ORF.
CYP4B1-CYP4X1 seems lost the major protein functional domain in Tgene partner, which is a essential gene due to the frame-shifted ORF.
CYP4B1-CYP4X1 seems lost the major protein functional domain in Tgene partner, which is a IUPHAR drug target due to the frame-shifted ORF.
* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

check button Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez
PartnerGeneGO IDGO termPubMed ID

check buttonFusion gene breakpoints across CYP4B1 (5'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

check buttonFusion gene breakpoints across CYP4X1 (3'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

check button Fusion gene information from two resources (ChiTars 5.0 and ChimerDB 4.0)
* All genome coordinats were lifted-over on hg19.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.
SourceDiseaseSampleHgeneHchrHbpHstrandTgeneTchrTbpTstrand
ChimerDB4Non-CancerSPNT_231CYP4B1chr1

47280936

+CYP4X1chr1

47495661

+
ChimerDB4Non-CancerSPNT_209CYP4B1chr1

47282853

+CYP4X1chr1

47495661

+
ChimerDB4Non-CancerSPNT_312CYP4B1chr1

47280936

+CYP4X1chr1

47495661

+
ChimerDB4Non-CancerSPNT_206CYP4B1chr1

47280936

+CYP4X1chr1

47495661

+
ChimerDB4Non-CancerSPNT_217CYP4B1chr1

47280936

+CYP4X1chr1

47495661

+
ChimerDB4Non-CancerSPNT_214CYP4B1chr1

47280936

+CYP4X1chr1

47495661

+
ChimerDB4Non-CancerSPNT_012CYP4B1chr1

47280936

+CYP4X1chr1

47495661

+


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Fusion Gene ORF analysis for CYP4B1-CYP4X1

check button Open reading frame (ORF) analsis of fusion genes based on Ensembl gene isoform structure.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.
ORFHenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrand
intron-3CDSENST00000546128ENST00000538609CYP4B1chr1

47280936

+CYP4X1chr1

47495661

+
intron-3CDSENST00000546128ENST00000371901CYP4B1chr1

47280936

+CYP4X1chr1

47495661

+
intron-intronENST00000546128ENST00000466294CYP4B1chr1

47280936

+CYP4X1chr1

47495661

+
Frame-shiftENST00000371923ENST00000538609CYP4B1chr1

47280936

+CYP4X1chr1

47495661

+
Frame-shiftENST00000371923ENST00000371901CYP4B1chr1

47280936

+CYP4X1chr1

47495661

+
5CDS-intronENST00000371923ENST00000466294CYP4B1chr1

47280936

+CYP4X1chr1

47495661

+
Frame-shiftENST00000271153ENST00000538609CYP4B1chr1

47280936

+CYP4X1chr1

47495661

+
Frame-shiftENST00000271153ENST00000371901CYP4B1chr1

47280936

+CYP4X1chr1

47495661

+
5CDS-intronENST00000271153ENST00000466294CYP4B1chr1

47280936

+CYP4X1chr1

47495661

+
Frame-shiftENST00000371919ENST00000538609CYP4B1chr1

47280936

+CYP4X1chr1

47495661

+
Frame-shiftENST00000371919ENST00000371901CYP4B1chr1

47280936

+CYP4X1chr1

47495661

+
5CDS-intronENST00000371919ENST00000466294CYP4B1chr1

47280936

+CYP4X1chr1

47495661

+
Frame-shiftENST00000452782ENST00000538609CYP4B1chr1

47280936

+CYP4X1chr1

47495661

+
Frame-shiftENST00000452782ENST00000371901CYP4B1chr1

47280936

+CYP4X1chr1

47495661

+
5CDS-intronENST00000452782ENST00000466294CYP4B1chr1

47280936

+CYP4X1chr1

47495661

+
intron-3CDSENST00000546128ENST00000538609CYP4B1chr1

47282853

+CYP4X1chr1

47495661

+
intron-3CDSENST00000546128ENST00000371901CYP4B1chr1

47282853

+CYP4X1chr1

47495661

+
intron-intronENST00000546128ENST00000466294CYP4B1chr1

47282853

+CYP4X1chr1

47495661

+
Frame-shiftENST00000371923ENST00000538609CYP4B1chr1

47282853

+CYP4X1chr1

47495661

+
Frame-shiftENST00000371923ENST00000371901CYP4B1chr1

47282853

+CYP4X1chr1

47495661

+
5CDS-intronENST00000371923ENST00000466294CYP4B1chr1

47282853

+CYP4X1chr1

47495661

+
Frame-shiftENST00000271153ENST00000538609CYP4B1chr1

47282853

+CYP4X1chr1

47495661

+
Frame-shiftENST00000271153ENST00000371901CYP4B1chr1

47282853

+CYP4X1chr1

47495661

+
5CDS-intronENST00000271153ENST00000466294CYP4B1chr1

47282853

+CYP4X1chr1

47495661

+
Frame-shiftENST00000371919ENST00000538609CYP4B1chr1

47282853

+CYP4X1chr1

47495661

+
Frame-shiftENST00000371919ENST00000371901CYP4B1chr1

47282853

+CYP4X1chr1

47495661

+
5CDS-intronENST00000371919ENST00000466294CYP4B1chr1

47282853

+CYP4X1chr1

47495661

+
Frame-shiftENST00000452782ENST00000538609CYP4B1chr1

47282853

+CYP4X1chr1

47495661

+
Frame-shiftENST00000452782ENST00000371901CYP4B1chr1

47282853

+CYP4X1chr1

47495661

+
5CDS-intronENST00000452782ENST00000466294CYP4B1chr1

47282853

+CYP4X1chr1

47495661

+

check buttonORFfinder result based on the fusion transcript sequence of in-frame fusion genes.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandSeq length
(transcript)		BP loci
(transcript)		Predicted start
(transcript)		Predicted stop
(transcript)		Seq length
(amino acids)

check buttonDeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandNo-coding scoreCoding score

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Fusion Genomic Features for CYP4B1-CYP4X1


check buttonFusionAI prediction of the potential fusion gene breakpoint based on the pre-mature RNA sequence context (+/- 5kb of individual partner genes, total 20kb length sequence). FusionAI is a fusion gene breakpoint classifier based on convolutional neural network by comparing the fusion positive and negative sequence context of ~ 20K fusion gene data. From here, we can have the relative potentency of the 20K genomic sequence how individual sequnce will be likely used as the gene fusion breakpoints.
HgeneHchrHbpHstrandTgeneTchrTbpTstrand1-pp (fusion gene breakpoint)
CYP4B1chr147282853+CYP4X1chr147495661+0.18936660.8106334
CYP4B1chr147280936+CYP4X1chr147495661+0.080393460.9196065
CYP4B1chr147282853+CYP4X1chr147495661+0.18936660.8106334
CYP4B1chr147280936+CYP4X1chr147495661+0.080393460.9196065

check buttonDistribution of 44 human genomic features loci across 20kb length fusion breakpoint regions. We integrated a total of 44 different types of human genomic feature loci information across five big categories including virus integration sites, repeats, structural variants, chromatin states, and gene expression regulation. More details are in help page.

check buttonDistribution of 44 human genomic features loci across 20kb length fusion breakpoint regions that are ovelapped with the top 1% feature importance score regions. More details are in help page.

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Fusion Protein Features for CYP4B1-CYP4X1


check button Go to

FGviewer for the breakpoints of :-:

.
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
FGviewer

check buttonMain function of each fusion partner protein. (from UniProt)
HgeneTgene
.CYP4X1

Q8N118

FUNCTION: Might normally function as a transcriptional repressor. EWS-fusion-proteins (EFPS) may play a role in the tumorigenic process. They may disturb gene expression by mimicking, or interfering with the normal function of CTD-POLII within the transcription initiation complex. They may also contribute to an aberrant activation of the fusion protein target genes.FUNCTION: A cytochrome P450 monooxygenase that selectively catalyzes the epoxidation of the last double bond of the arachidonoyl moiety of anandamide, potentially modulating endocannabinoid signaling. Has no hydroxylase activity toward various fatty acids, steroids and prostaglandins. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR; NADPH-ferrihemoprotein reductase). {ECO:0000269|PubMed:18549450}.

check buttonRetention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at

download page


* Minus value of BPloci means that the break pointn is located before the CDS.
- In-frame and retained protein feature among the 13 regional features.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note

- In-frame and not-retained protein feature among the 13 regional features.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note


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Fusion Gene Sequence for CYP4B1-CYP4X1


check button For in-frame fusion transcripts, we provide the fusion transcript sequences and fusion amino acid sequences. To have fusion amino acid sequence, we ran ORFfinder and chose the longest ORF among the all predicted ones.

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Fusion Gene PPI Analysis for CYP4B1-CYP4X1


check button Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in

ChiPPI page.


check button Protein-protein interactors with each fusion partner protein in wild-type (BIOGRID-3.4.160)
HgeneHgene's interactorsTgeneTgene's interactors


check button - Retained PPIs in in-frame fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenStill interaction with


check button - Lost PPIs in in-frame fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenInteraction lost with


check button - Retained PPIs, but lost function due to frame-shift fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenInteraction lost with


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Related Drugs for CYP4B1-CYP4X1


check button Drugs targeting genes involved in this fusion gene.
(DrugBank Version 5.1.8 2021-05-08)
PartnerGeneUniProtAccDrugBank IDDrug nameDrug activityDrug typeDrug status

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Related Diseases for CYP4B1-CYP4X1


check button Diseases associated with fusion partners.
(DisGeNet 4.0)
PartnerGeneDisease IDDisease name# pubmedsSource
HgeneCYP4B1C0005684Malignant neoplasm of urinary bladder1CTD_human
HgeneCYP4B1C0005695Bladder Neoplasm1CTD_human