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Center for Computational Systems Medicine
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Fusion Gene Summary

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Fusion Gene ORF analysis

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Fusion Genomic Features

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Fusion Protein Features

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Fusion Gene Sequence

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Fusion Gene PPI analysis

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Related Drugs

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Related Diseases

Fusion gene:AFAP1-RET (FusionGDB2 ID:2738)

Fusion Gene Summary for AFAP1-RET

check button Fusion gene summary
Fusion gene informationFusion gene name: AFAP1-RET
Fusion gene ID: 2738
HgeneTgene
Gene symbol

AFAP1

RET

Gene ID

60312

5979

Gene nameactin filament associated protein 1ret proto-oncogene
SynonymsAFAP|AFAP-110|AFAP110CDHF12|CDHR16|HSCR1|MEN2A|MEN2B|MTC1|PTC|RET-ELE1
Cytomap

4p16.1

10q11.21

Type of geneprotein-codingprotein-coding
Descriptionactin filament-associated protein 1110 kDa actin filament-associated proteinactin filament-associated protein, 110 kDaproto-oncogene tyrosine-protein kinase receptor RetRET receptor tyrosine kinasecadherin family member 12cadherin-related family member 16proto-oncogene c-Retrearranged during transfectionret proto-oncogene (multiple endocrine neoplasia and medullary
Modification date2020031320200322
UniProtAcc

Q8N556

RTL1

Ensembl transtripts involved in fusion geneENST00000360265, ENST00000358461, 
ENST00000420658, ENST00000382543, 
ENST00000513842, 		ENST00000355710, 
ENST00000340058, 
Fusion gene scores* DoF score11 X 17 X 7=130932 X 31 X 11=10912
# samples 1948
** MAII scorelog2(19/1309*10)=-2.78439377361429
possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs).
DoF>8 and MAII<0		log2(48/10912*10)=-4.50673733341565
possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs).
DoF>8 and MAII<0
Context

PubMed: AFAP1 [Title/Abstract] AND RET [Title/Abstract] AND fusion [Title/Abstract]

Most frequent breakpointAFAP1(7870348)-RET(43612031), # samples:1
Anticipated loss of major functional domain due to fusion event.
* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

check button Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez
PartnerGeneGO IDGO termPubMed ID
TgeneRET

GO:0030155

regulation of cell adhesion

21357690

TgeneRET

GO:0030335

positive regulation of cell migration

20702524

TgeneRET

GO:0033619

membrane protein proteolysis

21357690

TgeneRET

GO:0033630

positive regulation of cell adhesion mediated by integrin

20702524

TgeneRET

GO:0035860

glial cell-derived neurotrophic factor receptor signaling pathway

28953886

TgeneRET

GO:0043410

positive regulation of MAPK cascade

28846099


check buttonFusion gene breakpoints across AFAP1 (5'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

check buttonFusion gene breakpoints across RET (3'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

check button Fusion gene information from two resources (ChiTars 5.0 and ChimerDB 4.0)
* All genome coordinats were lifted-over on hg19.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.
SourceDiseaseSampleHgeneHchrHbpHstrandTgeneTchrTbpTstrand
ChiTaRS5.0N/AX56348AFAP1chr4

7870348

-RETchr10

43612031

+


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Fusion Gene ORF analysis for AFAP1-RET

check button Open reading frame (ORF) analsis of fusion genes based on Ensembl gene isoform structure.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.
ORFHenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrand
In-frameENST00000360265ENST00000355710AFAP1chr4

7870348

-RETchr10

43612031

+
In-frameENST00000360265ENST00000340058AFAP1chr4

7870348

-RETchr10

43612031

+
In-frameENST00000358461ENST00000355710AFAP1chr4

7870348

-RETchr10

43612031

+
In-frameENST00000358461ENST00000340058AFAP1chr4

7870348

-RETchr10

43612031

+
In-frameENST00000420658ENST00000355710AFAP1chr4

7870348

-RETchr10

43612031

+
In-frameENST00000420658ENST00000340058AFAP1chr4

7870348

-RETchr10

43612031

+
In-frameENST00000382543ENST00000355710AFAP1chr4

7870348

-RETchr10

43612031

+
In-frameENST00000382543ENST00000340058AFAP1chr4

7870348

-RETchr10

43612031

+
intron-3CDSENST00000513842ENST00000355710AFAP1chr4

7870348

-RETchr10

43612031

+
intron-3CDSENST00000513842ENST00000340058AFAP1chr4

7870348

-RETchr10

43612031

+

check buttonORFfinder result based on the fusion transcript sequence of in-frame fusion genes.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandSeq length
(transcript)		BP loci
(transcript)		Predicted start
(transcript)		Predicted stop
(transcript)		Seq length
(amino acids)
ENST00000360265AFAP1chr47870348-ENST00000355710RETchr1043612031+37514601871668493
ENST00000360265AFAP1chr47870348-ENST00000340058RETchr1043612031+22934601871542451
ENST00000358461AFAP1chr47870348-ENST00000355710RETchr1043612031+37894982731706477
ENST00000358461AFAP1chr47870348-ENST00000340058RETchr1043612031+23314982731580435
ENST00000420658AFAP1chr47870348-ENST00000355710RETchr1043612031+37894982731706477
ENST00000420658AFAP1chr47870348-ENST00000340058RETchr1043612031+23314982731580435
ENST00000382543AFAP1chr47870348-ENST00000355710RETchr1043612031+352022941437477
ENST00000382543AFAP1chr47870348-ENST00000340058RETchr1043612031+206222941311435

check buttonDeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandNo-coding scoreCoding score
ENST00000360265ENST00000355710AFAP1chr47870348-RETchr1043612031+0.0005470040.999453
ENST00000360265ENST00000340058AFAP1chr47870348-RETchr1043612031+0.001496750.9985033
ENST00000358461ENST00000355710AFAP1chr47870348-RETchr1043612031+0.0006871380.9993129
ENST00000358461ENST00000340058AFAP1chr47870348-RETchr1043612031+0.0018224250.9981775
ENST00000420658ENST00000355710AFAP1chr47870348-RETchr1043612031+0.0006871380.9993129
ENST00000420658ENST00000340058AFAP1chr47870348-RETchr1043612031+0.0018224250.9981775
ENST00000382543ENST00000355710AFAP1chr47870348-RETchr1043612031+0.0005306920.99946934
ENST00000382543ENST00000340058AFAP1chr47870348-RETchr1043612031+0.0016507210.9983493

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Fusion Genomic Features for AFAP1-RET


check buttonFusionAI prediction of the potential fusion gene breakpoint based on the pre-mature RNA sequence context (+/- 5kb of individual partner genes, total 20kb length sequence). FusionAI is a fusion gene breakpoint classifier based on convolutional neural network by comparing the fusion positive and negative sequence context of ~ 20K fusion gene data. From here, we can have the relative potentency of the 20K genomic sequence how individual sequnce will be likely used as the gene fusion breakpoints.
HgeneHchrHbpHstrandTgeneTchrTbpTstrand1-pp (fusion gene breakpoint)
AFAP1chr47870348-RETchr1043612031+1.25E-050.9999875
AFAP1chr47870348-RETchr1043612031+1.25E-050.9999875

check buttonDistribution of 44 human genomic features loci across 20kb length fusion breakpoint regions. We integrated a total of 44 different types of human genomic feature loci information across five big categories including virus integration sites, repeats, structural variants, chromatin states, and gene expression regulation. More details are in help page.

check buttonDistribution of 44 human genomic features loci across 20kb length fusion breakpoint regions that are ovelapped with the top 1% feature importance score regions. More details are in help page.

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Fusion Protein Features for AFAP1-RET


check button Go to

FGviewer for the breakpoints of chr4:7870348-chr10:43612031

.
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
FGviewer

check buttonMain function of each fusion partner protein. (from UniProt)
HgeneTgene
AFAP1

Q8N556

RET

RTL1

FUNCTION: Can cross-link actin filaments into both network and bundle structures (By similarity). May modulate changes in actin filament integrity and induce lamellipodia formation. May function as an adapter molecule that links other proteins, such as SRC and PKC to the actin cytoskeleton. Seems to play a role in the development and progression of prostate adenocarcinoma by regulating cell-matrix adhesions and migration in the cancer cells. {ECO:0000250, ECO:0000269|PubMed:15485829}.1358

check buttonRetention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at

download page


* Minus value of BPloci means that the break pointn is located before the CDS.
- In-frame and retained protein feature among the 13 regional features.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note
HgeneAFAP1chr4:7870348chr10:43612031ENST00000358461-31771_7475.0731.0MotifSH3-binding
HgeneAFAP1chr4:7870348chr10:43612031ENST00000360265-21671_7475.0731.0MotifSH3-binding
HgeneAFAP1chr4:7870348chr10:43612031ENST00000382543-21771_7475.0815.0MotifSH3-binding
HgeneAFAP1chr4:7870348chr10:43612031ENST00000420658-31871_7475.0815.0MotifSH3-binding
TgeneRETchr4:7870348chr10:43612031ENST000003400581019724_1016712.01073.0DomainProtein kinase
TgeneRETchr4:7870348chr10:43612031ENST000003557101020724_1016712.01115.0DomainProtein kinase
TgeneRETchr4:7870348chr10:43612031ENST000003400581019730_738712.01073.0Nucleotide bindingATP
TgeneRETchr4:7870348chr10:43612031ENST000003557101020730_738712.01115.0Nucleotide bindingATP
TgeneRETchr4:7870348chr10:43612031ENST000003400581019805_807712.01073.0RegionNote=Inhibitors binding
TgeneRETchr4:7870348chr10:43612031ENST000003557101020805_807712.01115.0RegionNote=Inhibitors binding

- In-frame and not-retained protein feature among the 13 regional features.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note
HgeneAFAP1chr4:7870348chr10:43612031ENST00000358461-317557_64875.0731.0Coiled coilOntology_term=ECO:0000255
HgeneAFAP1chr4:7870348chr10:43612031ENST00000360265-216557_64875.0731.0Coiled coilOntology_term=ECO:0000255
HgeneAFAP1chr4:7870348chr10:43612031ENST00000382543-217557_64875.0815.0Coiled coilOntology_term=ECO:0000255
HgeneAFAP1chr4:7870348chr10:43612031ENST00000420658-318557_64875.0815.0Coiled coilOntology_term=ECO:0000255
HgeneAFAP1chr4:7870348chr10:43612031ENST00000358461-31760_10675.0731.0Compositional biasNote=Pro-rich
HgeneAFAP1chr4:7870348chr10:43612031ENST00000360265-21660_10675.0731.0Compositional biasNote=Pro-rich
HgeneAFAP1chr4:7870348chr10:43612031ENST00000382543-21760_10675.0815.0Compositional biasNote=Pro-rich
HgeneAFAP1chr4:7870348chr10:43612031ENST00000420658-31860_10675.0815.0Compositional biasNote=Pro-rich
HgeneAFAP1chr4:7870348chr10:43612031ENST00000358461-317153_24975.0731.0DomainPH 1
HgeneAFAP1chr4:7870348chr10:43612031ENST00000358461-317347_44175.0731.0DomainPH 2
HgeneAFAP1chr4:7870348chr10:43612031ENST00000360265-216153_24975.0731.0DomainPH 1
HgeneAFAP1chr4:7870348chr10:43612031ENST00000360265-216347_44175.0731.0DomainPH 2
HgeneAFAP1chr4:7870348chr10:43612031ENST00000382543-217153_24975.0815.0DomainPH 1
HgeneAFAP1chr4:7870348chr10:43612031ENST00000382543-217347_44175.0815.0DomainPH 2
HgeneAFAP1chr4:7870348chr10:43612031ENST00000420658-318153_24975.0815.0DomainPH 1
HgeneAFAP1chr4:7870348chr10:43612031ENST00000420658-318347_44175.0815.0DomainPH 2
HgeneAFAP1chr4:7870348chr10:43612031ENST00000358461-317451_45675.0731.0MotifSH2-binding 2
HgeneAFAP1chr4:7870348chr10:43612031ENST00000358461-31794_9775.0731.0MotifSH2-binding 1
HgeneAFAP1chr4:7870348chr10:43612031ENST00000360265-216451_45675.0731.0MotifSH2-binding 2
HgeneAFAP1chr4:7870348chr10:43612031ENST00000360265-21694_9775.0731.0MotifSH2-binding 1
HgeneAFAP1chr4:7870348chr10:43612031ENST00000382543-217451_45675.0815.0MotifSH2-binding 2
HgeneAFAP1chr4:7870348chr10:43612031ENST00000382543-21794_9775.0815.0MotifSH2-binding 1
HgeneAFAP1chr4:7870348chr10:43612031ENST00000420658-318451_45675.0815.0MotifSH2-binding 2
HgeneAFAP1chr4:7870348chr10:43612031ENST00000420658-31894_9775.0815.0MotifSH2-binding 1
TgeneRETchr4:7870348chr10:43612031ENST000003400581019168_272712.01073.0DomainCadherin
TgeneRETchr4:7870348chr10:43612031ENST000003557101020168_272712.01115.0DomainCadherin
TgeneRETchr4:7870348chr10:43612031ENST00000340058101929_635712.01073.0Topological domainExtracellular
TgeneRETchr4:7870348chr10:43612031ENST000003400581019658_1114712.01073.0Topological domainCytoplasmic
TgeneRETchr4:7870348chr10:43612031ENST00000355710102029_635712.01115.0Topological domainExtracellular
TgeneRETchr4:7870348chr10:43612031ENST000003557101020658_1114712.01115.0Topological domainCytoplasmic
TgeneRETchr4:7870348chr10:43612031ENST000003400581019636_657712.01073.0TransmembraneHelical
TgeneRETchr4:7870348chr10:43612031ENST000003557101020636_657712.01115.0TransmembraneHelical


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Fusion Gene Sequence for AFAP1-RET


check button For in-frame fusion transcripts, we provide the fusion transcript sequences and fusion amino acid sequences. To have fusion amino acid sequence, we ran ORFfinder and chose the longest ORF among the all predicted ones.
>In-frame_ENST00000360265_ENST00000355710_X56348_AFAP1_chr4_7870348_-_RET_chr10_43612031_length(transcript)=3751nt_BP=460nt
CTCCCAGTTGAATTTTGCTGCCTTAAAGCAGAAGCAAAGGAGTAGTGCATTTGTTAAGAAGAAGACGCCTGAATCTTTTAGTGGACCTGC
TTTCAGCAAACTTGTTTTTTAGGTCAGAATACTATATCCAATGATCAAGCTAAACATGAAATGCTTCGAGTAAGACTCAGTATTCATACT
TTGACATTTGCAAATCACTGGGTCTAATAATACTCTTCCTCTTTTGTTGTCAGCAATGGAAGAGTTAATAGTTGAACTTCGTCTCTTTCT
TGAACTCCTGGACCATGAATATCTAACCTCAACTGTCAGGGAGAAAAAGGCAGTGATAACCAACATTCTGCTAAGAATACAGTCATCCAA
AGGTTTTGATGTGAAGGACCATGCTCAGAAGCAGGAGACCGCTAACAGCCTGCCAGCCCCTCCTCAGATGCCCCTGCCGGAGATCCCTCA
GCCCTGGCTGGAGGATCCAAAGTGGGAATTCCCTCGGAAGAACTTGGTTCTTGGAAAAACTCTAGGAGAAGGCGAATTTGGAAAAGTGGT
CAAGGCAACGGCCTTCCATCTGAAAGGCAGAGCAGGGTACACCACGGTGGCCGTGAAGATGCTGAAAGAGAACGCCTCCCCGAGTGAGCT
GCGAGACCTGCTGTCAGAGTTCAACGTCCTGAAGCAGGTCAACCACCCACATGTCATCAAATTGTATGGGGCCTGCAGCCAGGATGGCCC
GCTCCTCCTCATCGTGGAGTACGCCAAATACGGCTCCCTGCGGGGCTTCCTCCGCGAGAGCCGCAAAGTGGGGCCTGGCTACCTGGGCAG
TGGAGGCAGCCGCAACTCCAGCTCCCTGGACCACCCGGATGAGCGGGCCCTCACCATGGGCGACCTCATCTCATTTGCCTGGCAGATCTC
ACAGGGGATGCAGTATCTGGCCGAGATGAAGCTCGTTCATCGGGACTTGGCAGCCAGAAACATCCTGGTAGCTGAGGGGCGGAAGATGAA
GATTTCGGATTTCGGCTTGTCCCGAGATGTTTATGAAGAGGATTCCTACGTGAAGAGGAGCCAGGGTCGGATTCCAGTTAAATGGATGGC
AATTGAATCCCTTTTTGATCATATCTACACCACGCAAAGTGATGTATGGTCTTTTGGTGTCCTGCTGTGGGAGATCGTGACCCTAGGGGG
AAACCCCTATCCTGGGATTCCTCCTGAGCGGCTCTTCAACCTTCTGAAGACCGGCCACCGGATGGAGAGGCCAGACAACTGCAGCGAGGA
GATGTACCGCCTGATGCTGCAATGCTGGAAGCAGGAGCCGGACAAAAGGCCGGTGTTTGCGGACATCAGCAAAGACCTGGAGAAGATGAT
GGTTAAGAGGAGAGACTACTTGGACCTTGCGGCGTCCACTCCATCTGACTCCCTGATTTATGACGACGGCCTCTCAGAGGAGGAGACACC
GCTGGTGGACTGTAATAATGCCCCCCTCCCTCGAGCCCTCCCTTCCACATGGATTGAAAACAAACTCTATGGCATGTCAGACCCGAACTG
GCCTGGAGAGAGTCCTGTACCACTCACGAGAGCTGATGGCACTAACACTGGGTTTCCAAGATATCCAAATGATAGTGTATATGCTAACTG
GATGCTTTCACCCTCAGCGGCAAAATTAATGGACACGTTTGATAGTTAACATTTCTTTGTGAAAGGTAATGGACTCACAAGGGGAAGAAA
CATGCTGAGAATGGAAAGTCTACCGGCCCTTTCTTTGTGAACGTCACATTGGCCGAGCCGTGTTCAGTTCCCAGGTGGCAGACTCGTTTT
TGGTAGTTTGTTTTAACTTCCAAGGTGGTTTTACTTCTGATAGCCGGTGATTTTCCCTCCTAGCAGACATGCCACACCGGGTAAGAGCTC
TGAGTCTTAGTGGTTAAGCATTCCTTTCTCTTCAGTGCCCAGCAGCACCCAGTGTTGGTCTGTGTCCATCAGTGACCACCAACATTCTGT
GTTCACATGTGTGGGTCCAACACTTACTACCTGGTGTATGAAATTGGACCTGAACTGTTGGATTTTTCTAGTTGCCGCCAAACAAGGCAA
AAAAATTTAAACATGAAGCACACACACAAAAAAGGCAGTAGGAAAAATGCTGGCCCTGATGACCTGTCCTTATTCAGAATGAGAGACTGC
GGGGGGGGCCTGGGGGTAGTGTCAATGCCCCTCCAGGGCTGGAGGGGAAGAGGGGCCCCGAGGATGGGCCTGGGCTCAGCATTCGAGATC
TTGAGAATGATTTTTTTTAAATCATGCAACCTTTCCTTAGGAAGACATTTGGTTTTCATCATGATTAAGATGATTCCTAGATTTAGCACA
ATGGAGAGATTCCATGCCATCTTTACTATGTGGATGGTGGTATCAGGGAAGAGGGCTCACAAGACACATTTGTCCCCCGGGCCCACCACA
TCATCCTCACGTGTTCGGTACTGAGCAGCCACTACCCCTGATGAGAACAGTATGAAGAAAGGGGGCTGTTGGAGTCCCAGAATTGCTGAC
AGCAGAGGCTTTGCTGCTGTGAATCCCACCTGCCACCAGCCTGCAGCACACCCCACAGCCAAGTAGAGGCGAAAGCAGTGGCTCATCCTA
CCTGTTAGGAGCAGGTAGGGCTTGTACTCACTTTAATTTGAATCTTATCAACTTACTCATAAAGGGACAGGCTAGCTAGCTGTGTTAGAA
GTAGCAATGACAATGACCAAGGACTGCTACACCTCTGATTACAATTCTGATGTGAAAAAGATGGTGTTTGGCTCTTATAGAGCCTGTGTG
AAAGGCCCATGGATCAGCTCTTCCTGTGTTTGTAATTTAATGCTGCTACAAGATGTTTCTGTTTCTTAGATTCTGACCATGACTCATAAG
CTTCTTGTCATTCTTCATTGCTTGTTTGTGGTCACAGATGCACAACACTCCTCCAGTCTTGTGGGGGCAGCTTTTGGGAAGTCTCAGCAG
CTCTTCTGGCTGTGTTGTCAGCACTGTAACTTCGCAGAAAAGAGTCGGATTACCAAAACACTGCCTGCTCTTCAGACTTAAAGCACTGAT
AGGACTTAAAATAGTCTCATTCAAATACTGTATTTTATATAGGCATTTCACAAAAACAGCAAAATTGTGGCATTTTGTGAGGCCAAGGCT
TGGATGCGTGTGTAATAGAGCCTTGTGGTGTGTGCGCACACACCCAGAGGGAGAGTTTGAAAAATGCTTATTGGACACGTAACCTGGCTC
TAATTTGGGCTGTTTTTCAGATACACTGTGATAAGTTCTTTTACAAATATCTATAGACATGGTAAACTTTTGGTTTTCAGATATGCTTAA
TGATAGTCTTACTAAATGCAGAAATAAGAATAAACTTTCTCAAATTATTAAAAATGCCTACACAGTAAGTGTGAATTGCTGCAACAGGTT
TGTTCTCAGGAGGGTAAGAACTCCAGGTCTAAACAGCTGACCCAGTGATGGGGAATTTATCCTTGACCAATTTATCCTTGACCAATAACC
TAATTGTCTATTCCTGAGTTATAAAAGTCCCCATCCTTATTAGCTCTACTGGAATTTTCATACACGTAAATGCAGAAGTTACTAAGTATT
AAGTATTACTGAGTATTAAGTAGTAATCTGTCAGTTATTAAAATTTGTAAAATCTATTTATGAAAGGTCATTAAACCAGATCATGTTCCT

>In-frame_ENST00000360265_ENST00000355710_X56348_AFAP1_chr4_7870348_-_RET_chr10_43612031_length(amino acids)=493AA_start in transcript=187_stop in transcript=1668
MQITGSNNTLPLLLSAMEELIVELRLFLELLDHEYLTSTVREKKAVITNILLRIQSSKGFDVKDHAQKQETANSLPAPPQMPLPEIPQPW
LEDPKWEFPRKNLVLGKTLGEGEFGKVVKATAFHLKGRAGYTTVAVKMLKENASPSELRDLLSEFNVLKQVNHPHVIKLYGACSQDGPLL
LIVEYAKYGSLRGFLRESRKVGPGYLGSGGSRNSSSLDHPDERALTMGDLISFAWQISQGMQYLAEMKLVHRDLAARNILVAEGRKMKIS
DFGLSRDVYEEDSYVKRSQGRIPVKWMAIESLFDHIYTTQSDVWSFGVLLWEIVTLGGNPYPGIPPERLFNLLKTGHRMERPDNCSEEMY
RLMLQCWKQEPDKRPVFADISKDLEKMMVKRRDYLDLAASTPSDSLIYDDGLSEEETPLVDCNNAPLPRALPSTWIENKLYGMSDPNWPG

--------------------------------------------------------------
>In-frame_ENST00000360265_ENST00000340058_X56348_AFAP1_chr4_7870348_-_RET_chr10_43612031_length(transcript)=2293nt_BP=460nt
CTCCCAGTTGAATTTTGCTGCCTTAAAGCAGAAGCAAAGGAGTAGTGCATTTGTTAAGAAGAAGACGCCTGAATCTTTTAGTGGACCTGC
TTTCAGCAAACTTGTTTTTTAGGTCAGAATACTATATCCAATGATCAAGCTAAACATGAAATGCTTCGAGTAAGACTCAGTATTCATACT
TTGACATTTGCAAATCACTGGGTCTAATAATACTCTTCCTCTTTTGTTGTCAGCAATGGAAGAGTTAATAGTTGAACTTCGTCTCTTTCT
TGAACTCCTGGACCATGAATATCTAACCTCAACTGTCAGGGAGAAAAAGGCAGTGATAACCAACATTCTGCTAAGAATACAGTCATCCAA
AGGTTTTGATGTGAAGGACCATGCTCAGAAGCAGGAGACCGCTAACAGCCTGCCAGCCCCTCCTCAGATGCCCCTGCCGGAGATCCCTCA
GCCCTGGCTGGAGGATCCAAAGTGGGAATTCCCTCGGAAGAACTTGGTTCTTGGAAAAACTCTAGGAGAAGGCGAATTTGGAAAAGTGGT
CAAGGCAACGGCCTTCCATCTGAAAGGCAGAGCAGGGTACACCACGGTGGCCGTGAAGATGCTGAAAGAGAACGCCTCCCCGAGTGAGCT
GCGAGACCTGCTGTCAGAGTTCAACGTCCTGAAGCAGGTCAACCACCCACATGTCATCAAATTGTATGGGGCCTGCAGCCAGGATGGCCC
GCTCCTCCTCATCGTGGAGTACGCCAAATACGGCTCCCTGCGGGGCTTCCTCCGCGAGAGCCGCAAAGTGGGGCCTGGCTACCTGGGCAG
TGGAGGCAGCCGCAACTCCAGCTCCCTGGACCACCCGGATGAGCGGGCCCTCACCATGGGCGACCTCATCTCATTTGCCTGGCAGATCTC
ACAGGGGATGCAGTATCTGGCCGAGATGAAGCTCGTTCATCGGGACTTGGCAGCCAGAAACATCCTGGTAGCTGAGGGGCGGAAGATGAA
GATTTCGGATTTCGGCTTGTCCCGAGATGTTTATGAAGAGGATTCCTACGTGAAGAGGAGCCAGGGTCGGATTCCAGTTAAATGGATGGC
AATTGAATCCCTTTTTGATCATATCTACACCACGCAAAGTGATGTATGGTCTTTTGGTGTCCTGCTGTGGGAGATCGTGACCCTAGGGGG
AAACCCCTATCCTGGGATTCCTCCTGAGCGGCTCTTCAACCTTCTGAAGACCGGCCACCGGATGGAGAGGCCAGACAACTGCAGCGAGGA
GATGTACCGCCTGATGCTGCAATGCTGGAAGCAGGAGCCGGACAAAAGGCCGGTGTTTGCGGACATCAGCAAAGACCTGGAGAAGATGAT
GGTTAAGAGGAGAGACTACTTGGACCTTGCGGCGTCCACTCCATCTGACTCCCTGATTTATGACGACGGCCTCTCAGAGGAGGAGACACC
GCTGGTGGACTGTAATAATGCCCCCCTCCCTCGAGCCCTCCCTTCCACATGGATTGAAAACAAACTCTATGGTAGAATTTCCCATGCATT
TACTAGATTCTAGCACCGCTGTCCCCTTTGCACTATCCTTCCTCTCTGTGATGCTTTTTAAAAATGTTTCTGGTCTGAACAAAACCAAAG
TCTGCTCTGAACCTTTTTATTTGTAAATGTCTGACTTTGCATCCAGTTTACATTTAGGCATTATTGCAACTATGTTTTTCTAAAAGGATG
TGAAAATAAGTGTAATTACCACATTGCCCAGCAACTTAGGATGGTAGAGGAAAAAACAGATCAGGGCGGAACTCTCAGGGGAGACCAAGA
ACAGGTTGAATAAGGCGCTTCTGGGGTGGGAATCAAGTCATAGTACTTCTACTTTAACTAAGTGGATAAATATACAAATCTGGGGAGGTA
TTCAGTTGAGAAAGGAGCCACCAGCACCACTCAGCCTGCACTGGGAGCACAGCCAGGTTCCCCCAGACCCCTCCTGGGCAGGCAGGTGCC
TCTCAGAGGCCACCCGGCACTGGCGAGCAGCCACTGGCCAAGCCTCAGCCCCAGTCCCAGCCACATGTCCTCCATCAGGGGTAGCGAGGT
TGCAGGAGCTGGCTGGCCCTGGGAGGACGCACCCCCACTGCTGTTTTCACATCCTTTCCCTTACCCACCTTCAGGACGGTTGTCACTTAT
GAAGTCAGTGCTAAAGCTGGAGCAGTTGCTTTTTGAAAGAACATGGTCTGTGGTGCTGTGGTCTTACAATGGACAGTAAATATGGTTCTT

>In-frame_ENST00000360265_ENST00000340058_X56348_AFAP1_chr4_7870348_-_RET_chr10_43612031_length(amino acids)=451AA_start in transcript=187_stop in transcript=1542
MQITGSNNTLPLLLSAMEELIVELRLFLELLDHEYLTSTVREKKAVITNILLRIQSSKGFDVKDHAQKQETANSLPAPPQMPLPEIPQPW
LEDPKWEFPRKNLVLGKTLGEGEFGKVVKATAFHLKGRAGYTTVAVKMLKENASPSELRDLLSEFNVLKQVNHPHVIKLYGACSQDGPLL
LIVEYAKYGSLRGFLRESRKVGPGYLGSGGSRNSSSLDHPDERALTMGDLISFAWQISQGMQYLAEMKLVHRDLAARNILVAEGRKMKIS
DFGLSRDVYEEDSYVKRSQGRIPVKWMAIESLFDHIYTTQSDVWSFGVLLWEIVTLGGNPYPGIPPERLFNLLKTGHRMERPDNCSEEMY
RLMLQCWKQEPDKRPVFADISKDLEKMMVKRRDYLDLAASTPSDSLIYDDGLSEEETPLVDCNNAPLPRALPSTWIENKLYGRISHAFTR

--------------------------------------------------------------
>In-frame_ENST00000358461_ENST00000355710_X56348_AFAP1_chr4_7870348_-_RET_chr10_43612031_length(transcript)=3789nt_BP=498nt
GGGCGCCCGGCTCCGCTGGGGGCGGGACGGCGGGCGGCGGGCGGGGCCGTGTGCGCCTGCGTGGTGTCTGTCCCGGCCGCAGCCCCACGC
CGGCTGCAGCTCCGCGAGCCGAGCCGGATCTCGAGCGGGGTACACGGGCTGAGGCGGCGGCGGCGGCCCCGCCCCGGGGCTCCATTGTTA
AGGCGGCTGCGGCTCTGTCGGCTGTTCAGCTGCGCTCCAGTCGGCCCAGGCGCCGCGGCGAGGAGCGAGCGCTGCGAGGTGGCGGCCTGA
GCAATGGAAGAGTTAATAGTTGAACTTCGTCTCTTTCTTGAACTCCTGGACCATGAATATCTAACCTCAACTGTCAGGGAGAAAAAGGCA
GTGATAACCAACATTCTGCTAAGAATACAGTCATCCAAAGGTTTTGATGTGAAGGACCATGCTCAGAAGCAGGAGACCGCTAACAGCCTG
CCAGCCCCTCCTCAGATGCCCCTGCCGGAGATCCCTCAGCCCTGGCTGGAGGATCCAAAGTGGGAATTCCCTCGGAAGAACTTGGTTCTT
GGAAAAACTCTAGGAGAAGGCGAATTTGGAAAAGTGGTCAAGGCAACGGCCTTCCATCTGAAAGGCAGAGCAGGGTACACCACGGTGGCC
GTGAAGATGCTGAAAGAGAACGCCTCCCCGAGTGAGCTGCGAGACCTGCTGTCAGAGTTCAACGTCCTGAAGCAGGTCAACCACCCACAT
GTCATCAAATTGTATGGGGCCTGCAGCCAGGATGGCCCGCTCCTCCTCATCGTGGAGTACGCCAAATACGGCTCCCTGCGGGGCTTCCTC
CGCGAGAGCCGCAAAGTGGGGCCTGGCTACCTGGGCAGTGGAGGCAGCCGCAACTCCAGCTCCCTGGACCACCCGGATGAGCGGGCCCTC
ACCATGGGCGACCTCATCTCATTTGCCTGGCAGATCTCACAGGGGATGCAGTATCTGGCCGAGATGAAGCTCGTTCATCGGGACTTGGCA
GCCAGAAACATCCTGGTAGCTGAGGGGCGGAAGATGAAGATTTCGGATTTCGGCTTGTCCCGAGATGTTTATGAAGAGGATTCCTACGTG
AAGAGGAGCCAGGGTCGGATTCCAGTTAAATGGATGGCAATTGAATCCCTTTTTGATCATATCTACACCACGCAAAGTGATGTATGGTCT
TTTGGTGTCCTGCTGTGGGAGATCGTGACCCTAGGGGGAAACCCCTATCCTGGGATTCCTCCTGAGCGGCTCTTCAACCTTCTGAAGACC
GGCCACCGGATGGAGAGGCCAGACAACTGCAGCGAGGAGATGTACCGCCTGATGCTGCAATGCTGGAAGCAGGAGCCGGACAAAAGGCCG
GTGTTTGCGGACATCAGCAAAGACCTGGAGAAGATGATGGTTAAGAGGAGAGACTACTTGGACCTTGCGGCGTCCACTCCATCTGACTCC
CTGATTTATGACGACGGCCTCTCAGAGGAGGAGACACCGCTGGTGGACTGTAATAATGCCCCCCTCCCTCGAGCCCTCCCTTCCACATGG
ATTGAAAACAAACTCTATGGCATGTCAGACCCGAACTGGCCTGGAGAGAGTCCTGTACCACTCACGAGAGCTGATGGCACTAACACTGGG
TTTCCAAGATATCCAAATGATAGTGTATATGCTAACTGGATGCTTTCACCCTCAGCGGCAAAATTAATGGACACGTTTGATAGTTAACAT
TTCTTTGTGAAAGGTAATGGACTCACAAGGGGAAGAAACATGCTGAGAATGGAAAGTCTACCGGCCCTTTCTTTGTGAACGTCACATTGG
CCGAGCCGTGTTCAGTTCCCAGGTGGCAGACTCGTTTTTGGTAGTTTGTTTTAACTTCCAAGGTGGTTTTACTTCTGATAGCCGGTGATT
TTCCCTCCTAGCAGACATGCCACACCGGGTAAGAGCTCTGAGTCTTAGTGGTTAAGCATTCCTTTCTCTTCAGTGCCCAGCAGCACCCAG
TGTTGGTCTGTGTCCATCAGTGACCACCAACATTCTGTGTTCACATGTGTGGGTCCAACACTTACTACCTGGTGTATGAAATTGGACCTG
AACTGTTGGATTTTTCTAGTTGCCGCCAAACAAGGCAAAAAAATTTAAACATGAAGCACACACACAAAAAAGGCAGTAGGAAAAATGCTG
GCCCTGATGACCTGTCCTTATTCAGAATGAGAGACTGCGGGGGGGGCCTGGGGGTAGTGTCAATGCCCCTCCAGGGCTGGAGGGGAAGAG
GGGCCCCGAGGATGGGCCTGGGCTCAGCATTCGAGATCTTGAGAATGATTTTTTTTAAATCATGCAACCTTTCCTTAGGAAGACATTTGG
TTTTCATCATGATTAAGATGATTCCTAGATTTAGCACAATGGAGAGATTCCATGCCATCTTTACTATGTGGATGGTGGTATCAGGGAAGA
GGGCTCACAAGACACATTTGTCCCCCGGGCCCACCACATCATCCTCACGTGTTCGGTACTGAGCAGCCACTACCCCTGATGAGAACAGTA
TGAAGAAAGGGGGCTGTTGGAGTCCCAGAATTGCTGACAGCAGAGGCTTTGCTGCTGTGAATCCCACCTGCCACCAGCCTGCAGCACACC
CCACAGCCAAGTAGAGGCGAAAGCAGTGGCTCATCCTACCTGTTAGGAGCAGGTAGGGCTTGTACTCACTTTAATTTGAATCTTATCAAC
TTACTCATAAAGGGACAGGCTAGCTAGCTGTGTTAGAAGTAGCAATGACAATGACCAAGGACTGCTACACCTCTGATTACAATTCTGATG
TGAAAAAGATGGTGTTTGGCTCTTATAGAGCCTGTGTGAAAGGCCCATGGATCAGCTCTTCCTGTGTTTGTAATTTAATGCTGCTACAAG
ATGTTTCTGTTTCTTAGATTCTGACCATGACTCATAAGCTTCTTGTCATTCTTCATTGCTTGTTTGTGGTCACAGATGCACAACACTCCT
CCAGTCTTGTGGGGGCAGCTTTTGGGAAGTCTCAGCAGCTCTTCTGGCTGTGTTGTCAGCACTGTAACTTCGCAGAAAAGAGTCGGATTA
CCAAAACACTGCCTGCTCTTCAGACTTAAAGCACTGATAGGACTTAAAATAGTCTCATTCAAATACTGTATTTTATATAGGCATTTCACA
AAAACAGCAAAATTGTGGCATTTTGTGAGGCCAAGGCTTGGATGCGTGTGTAATAGAGCCTTGTGGTGTGTGCGCACACACCCAGAGGGA
GAGTTTGAAAAATGCTTATTGGACACGTAACCTGGCTCTAATTTGGGCTGTTTTTCAGATACACTGTGATAAGTTCTTTTACAAATATCT
ATAGACATGGTAAACTTTTGGTTTTCAGATATGCTTAATGATAGTCTTACTAAATGCAGAAATAAGAATAAACTTTCTCAAATTATTAAA
AATGCCTACACAGTAAGTGTGAATTGCTGCAACAGGTTTGTTCTCAGGAGGGTAAGAACTCCAGGTCTAAACAGCTGACCCAGTGATGGG
GAATTTATCCTTGACCAATTTATCCTTGACCAATAACCTAATTGTCTATTCCTGAGTTATAAAAGTCCCCATCCTTATTAGCTCTACTGG
AATTTTCATACACGTAAATGCAGAAGTTACTAAGTATTAAGTATTACTGAGTATTAAGTAGTAATCTGTCAGTTATTAAAATTTGTAAAA
TCTATTTATGAAAGGTCATTAAACCAGATCATGTTCCTTTTTTTGTAATCAAGGTGACTAAGAAAATCAGTTGTGTAAATAAAATCATGT

>In-frame_ENST00000358461_ENST00000355710_X56348_AFAP1_chr4_7870348_-_RET_chr10_43612031_length(amino acids)=477AA_start in transcript=273_stop in transcript=1706
MEELIVELRLFLELLDHEYLTSTVREKKAVITNILLRIQSSKGFDVKDHAQKQETANSLPAPPQMPLPEIPQPWLEDPKWEFPRKNLVLG
KTLGEGEFGKVVKATAFHLKGRAGYTTVAVKMLKENASPSELRDLLSEFNVLKQVNHPHVIKLYGACSQDGPLLLIVEYAKYGSLRGFLR
ESRKVGPGYLGSGGSRNSSSLDHPDERALTMGDLISFAWQISQGMQYLAEMKLVHRDLAARNILVAEGRKMKISDFGLSRDVYEEDSYVK
RSQGRIPVKWMAIESLFDHIYTTQSDVWSFGVLLWEIVTLGGNPYPGIPPERLFNLLKTGHRMERPDNCSEEMYRLMLQCWKQEPDKRPV
FADISKDLEKMMVKRRDYLDLAASTPSDSLIYDDGLSEEETPLVDCNNAPLPRALPSTWIENKLYGMSDPNWPGESPVPLTRADGTNTGF

--------------------------------------------------------------
>In-frame_ENST00000358461_ENST00000340058_X56348_AFAP1_chr4_7870348_-_RET_chr10_43612031_length(transcript)=2331nt_BP=498nt
GGGCGCCCGGCTCCGCTGGGGGCGGGACGGCGGGCGGCGGGCGGGGCCGTGTGCGCCTGCGTGGTGTCTGTCCCGGCCGCAGCCCCACGC
CGGCTGCAGCTCCGCGAGCCGAGCCGGATCTCGAGCGGGGTACACGGGCTGAGGCGGCGGCGGCGGCCCCGCCCCGGGGCTCCATTGTTA
AGGCGGCTGCGGCTCTGTCGGCTGTTCAGCTGCGCTCCAGTCGGCCCAGGCGCCGCGGCGAGGAGCGAGCGCTGCGAGGTGGCGGCCTGA
GCAATGGAAGAGTTAATAGTTGAACTTCGTCTCTTTCTTGAACTCCTGGACCATGAATATCTAACCTCAACTGTCAGGGAGAAAAAGGCA
GTGATAACCAACATTCTGCTAAGAATACAGTCATCCAAAGGTTTTGATGTGAAGGACCATGCTCAGAAGCAGGAGACCGCTAACAGCCTG
CCAGCCCCTCCTCAGATGCCCCTGCCGGAGATCCCTCAGCCCTGGCTGGAGGATCCAAAGTGGGAATTCCCTCGGAAGAACTTGGTTCTT
GGAAAAACTCTAGGAGAAGGCGAATTTGGAAAAGTGGTCAAGGCAACGGCCTTCCATCTGAAAGGCAGAGCAGGGTACACCACGGTGGCC
GTGAAGATGCTGAAAGAGAACGCCTCCCCGAGTGAGCTGCGAGACCTGCTGTCAGAGTTCAACGTCCTGAAGCAGGTCAACCACCCACAT
GTCATCAAATTGTATGGGGCCTGCAGCCAGGATGGCCCGCTCCTCCTCATCGTGGAGTACGCCAAATACGGCTCCCTGCGGGGCTTCCTC
CGCGAGAGCCGCAAAGTGGGGCCTGGCTACCTGGGCAGTGGAGGCAGCCGCAACTCCAGCTCCCTGGACCACCCGGATGAGCGGGCCCTC
ACCATGGGCGACCTCATCTCATTTGCCTGGCAGATCTCACAGGGGATGCAGTATCTGGCCGAGATGAAGCTCGTTCATCGGGACTTGGCA
GCCAGAAACATCCTGGTAGCTGAGGGGCGGAAGATGAAGATTTCGGATTTCGGCTTGTCCCGAGATGTTTATGAAGAGGATTCCTACGTG
AAGAGGAGCCAGGGTCGGATTCCAGTTAAATGGATGGCAATTGAATCCCTTTTTGATCATATCTACACCACGCAAAGTGATGTATGGTCT
TTTGGTGTCCTGCTGTGGGAGATCGTGACCCTAGGGGGAAACCCCTATCCTGGGATTCCTCCTGAGCGGCTCTTCAACCTTCTGAAGACC
GGCCACCGGATGGAGAGGCCAGACAACTGCAGCGAGGAGATGTACCGCCTGATGCTGCAATGCTGGAAGCAGGAGCCGGACAAAAGGCCG
GTGTTTGCGGACATCAGCAAAGACCTGGAGAAGATGATGGTTAAGAGGAGAGACTACTTGGACCTTGCGGCGTCCACTCCATCTGACTCC
CTGATTTATGACGACGGCCTCTCAGAGGAGGAGACACCGCTGGTGGACTGTAATAATGCCCCCCTCCCTCGAGCCCTCCCTTCCACATGG
ATTGAAAACAAACTCTATGGTAGAATTTCCCATGCATTTACTAGATTCTAGCACCGCTGTCCCCTTTGCACTATCCTTCCTCTCTGTGAT
GCTTTTTAAAAATGTTTCTGGTCTGAACAAAACCAAAGTCTGCTCTGAACCTTTTTATTTGTAAATGTCTGACTTTGCATCCAGTTTACA
TTTAGGCATTATTGCAACTATGTTTTTCTAAAAGGATGTGAAAATAAGTGTAATTACCACATTGCCCAGCAACTTAGGATGGTAGAGGAA
AAAACAGATCAGGGCGGAACTCTCAGGGGAGACCAAGAACAGGTTGAATAAGGCGCTTCTGGGGTGGGAATCAAGTCATAGTACTTCTAC
TTTAACTAAGTGGATAAATATACAAATCTGGGGAGGTATTCAGTTGAGAAAGGAGCCACCAGCACCACTCAGCCTGCACTGGGAGCACAG
CCAGGTTCCCCCAGACCCCTCCTGGGCAGGCAGGTGCCTCTCAGAGGCCACCCGGCACTGGCGAGCAGCCACTGGCCAAGCCTCAGCCCC
AGTCCCAGCCACATGTCCTCCATCAGGGGTAGCGAGGTTGCAGGAGCTGGCTGGCCCTGGGAGGACGCACCCCCACTGCTGTTTTCACAT
CCTTTCCCTTACCCACCTTCAGGACGGTTGTCACTTATGAAGTCAGTGCTAAAGCTGGAGCAGTTGCTTTTTGAAAGAACATGGTCTGTG

>In-frame_ENST00000358461_ENST00000340058_X56348_AFAP1_chr4_7870348_-_RET_chr10_43612031_length(amino acids)=435AA_start in transcript=273_stop in transcript=1580
MEELIVELRLFLELLDHEYLTSTVREKKAVITNILLRIQSSKGFDVKDHAQKQETANSLPAPPQMPLPEIPQPWLEDPKWEFPRKNLVLG
KTLGEGEFGKVVKATAFHLKGRAGYTTVAVKMLKENASPSELRDLLSEFNVLKQVNHPHVIKLYGACSQDGPLLLIVEYAKYGSLRGFLR
ESRKVGPGYLGSGGSRNSSSLDHPDERALTMGDLISFAWQISQGMQYLAEMKLVHRDLAARNILVAEGRKMKISDFGLSRDVYEEDSYVK
RSQGRIPVKWMAIESLFDHIYTTQSDVWSFGVLLWEIVTLGGNPYPGIPPERLFNLLKTGHRMERPDNCSEEMYRLMLQCWKQEPDKRPV

--------------------------------------------------------------
>In-frame_ENST00000420658_ENST00000355710_X56348_AFAP1_chr4_7870348_-_RET_chr10_43612031_length(transcript)=3789nt_BP=498nt
GGGCGCCCGGCTCCGCTGGGGGCGGGACGGCGGGCGGCGGGCGGGGCCGTGTGCGCCTGCGTGGTGTCTGTCCCGGCCGCAGCCCCACGC
CGGCTGCAGCTCCGCGAGCCGAGCCGGATCTCGAGCGGGGTACACGGGCTGAGGCGGCGGCGGCGGCCCCGCCCCGGGGCTCCATTGTTA
AGGCGGCTGCGGCTCTGTCGGCTGTTCAGCTGCGCTCCAGTCGGCCCAGGCGCCGCGGCGAGGAGCGAGCGCTGCGAGGTGGCGGCCTGA
GCAATGGAAGAGTTAATAGTTGAACTTCGTCTCTTTCTTGAACTCCTGGACCATGAATATCTAACCTCAACTGTCAGGGAGAAAAAGGCA
GTGATAACCAACATTCTGCTAAGAATACAGTCATCCAAAGGTTTTGATGTGAAGGACCATGCTCAGAAGCAGGAGACCGCTAACAGCCTG
CCAGCCCCTCCTCAGATGCCCCTGCCGGAGATCCCTCAGCCCTGGCTGGAGGATCCAAAGTGGGAATTCCCTCGGAAGAACTTGGTTCTT
GGAAAAACTCTAGGAGAAGGCGAATTTGGAAAAGTGGTCAAGGCAACGGCCTTCCATCTGAAAGGCAGAGCAGGGTACACCACGGTGGCC
GTGAAGATGCTGAAAGAGAACGCCTCCCCGAGTGAGCTGCGAGACCTGCTGTCAGAGTTCAACGTCCTGAAGCAGGTCAACCACCCACAT
GTCATCAAATTGTATGGGGCCTGCAGCCAGGATGGCCCGCTCCTCCTCATCGTGGAGTACGCCAAATACGGCTCCCTGCGGGGCTTCCTC
CGCGAGAGCCGCAAAGTGGGGCCTGGCTACCTGGGCAGTGGAGGCAGCCGCAACTCCAGCTCCCTGGACCACCCGGATGAGCGGGCCCTC
ACCATGGGCGACCTCATCTCATTTGCCTGGCAGATCTCACAGGGGATGCAGTATCTGGCCGAGATGAAGCTCGTTCATCGGGACTTGGCA
GCCAGAAACATCCTGGTAGCTGAGGGGCGGAAGATGAAGATTTCGGATTTCGGCTTGTCCCGAGATGTTTATGAAGAGGATTCCTACGTG
AAGAGGAGCCAGGGTCGGATTCCAGTTAAATGGATGGCAATTGAATCCCTTTTTGATCATATCTACACCACGCAAAGTGATGTATGGTCT
TTTGGTGTCCTGCTGTGGGAGATCGTGACCCTAGGGGGAAACCCCTATCCTGGGATTCCTCCTGAGCGGCTCTTCAACCTTCTGAAGACC
GGCCACCGGATGGAGAGGCCAGACAACTGCAGCGAGGAGATGTACCGCCTGATGCTGCAATGCTGGAAGCAGGAGCCGGACAAAAGGCCG
GTGTTTGCGGACATCAGCAAAGACCTGGAGAAGATGATGGTTAAGAGGAGAGACTACTTGGACCTTGCGGCGTCCACTCCATCTGACTCC
CTGATTTATGACGACGGCCTCTCAGAGGAGGAGACACCGCTGGTGGACTGTAATAATGCCCCCCTCCCTCGAGCCCTCCCTTCCACATGG
ATTGAAAACAAACTCTATGGCATGTCAGACCCGAACTGGCCTGGAGAGAGTCCTGTACCACTCACGAGAGCTGATGGCACTAACACTGGG
TTTCCAAGATATCCAAATGATAGTGTATATGCTAACTGGATGCTTTCACCCTCAGCGGCAAAATTAATGGACACGTTTGATAGTTAACAT
TTCTTTGTGAAAGGTAATGGACTCACAAGGGGAAGAAACATGCTGAGAATGGAAAGTCTACCGGCCCTTTCTTTGTGAACGTCACATTGG
CCGAGCCGTGTTCAGTTCCCAGGTGGCAGACTCGTTTTTGGTAGTTTGTTTTAACTTCCAAGGTGGTTTTACTTCTGATAGCCGGTGATT
TTCCCTCCTAGCAGACATGCCACACCGGGTAAGAGCTCTGAGTCTTAGTGGTTAAGCATTCCTTTCTCTTCAGTGCCCAGCAGCACCCAG
TGTTGGTCTGTGTCCATCAGTGACCACCAACATTCTGTGTTCACATGTGTGGGTCCAACACTTACTACCTGGTGTATGAAATTGGACCTG
AACTGTTGGATTTTTCTAGTTGCCGCCAAACAAGGCAAAAAAATTTAAACATGAAGCACACACACAAAAAAGGCAGTAGGAAAAATGCTG
GCCCTGATGACCTGTCCTTATTCAGAATGAGAGACTGCGGGGGGGGCCTGGGGGTAGTGTCAATGCCCCTCCAGGGCTGGAGGGGAAGAG
GGGCCCCGAGGATGGGCCTGGGCTCAGCATTCGAGATCTTGAGAATGATTTTTTTTAAATCATGCAACCTTTCCTTAGGAAGACATTTGG
TTTTCATCATGATTAAGATGATTCCTAGATTTAGCACAATGGAGAGATTCCATGCCATCTTTACTATGTGGATGGTGGTATCAGGGAAGA
GGGCTCACAAGACACATTTGTCCCCCGGGCCCACCACATCATCCTCACGTGTTCGGTACTGAGCAGCCACTACCCCTGATGAGAACAGTA
TGAAGAAAGGGGGCTGTTGGAGTCCCAGAATTGCTGACAGCAGAGGCTTTGCTGCTGTGAATCCCACCTGCCACCAGCCTGCAGCACACC
CCACAGCCAAGTAGAGGCGAAAGCAGTGGCTCATCCTACCTGTTAGGAGCAGGTAGGGCTTGTACTCACTTTAATTTGAATCTTATCAAC
TTACTCATAAAGGGACAGGCTAGCTAGCTGTGTTAGAAGTAGCAATGACAATGACCAAGGACTGCTACACCTCTGATTACAATTCTGATG
TGAAAAAGATGGTGTTTGGCTCTTATAGAGCCTGTGTGAAAGGCCCATGGATCAGCTCTTCCTGTGTTTGTAATTTAATGCTGCTACAAG
ATGTTTCTGTTTCTTAGATTCTGACCATGACTCATAAGCTTCTTGTCATTCTTCATTGCTTGTTTGTGGTCACAGATGCACAACACTCCT
CCAGTCTTGTGGGGGCAGCTTTTGGGAAGTCTCAGCAGCTCTTCTGGCTGTGTTGTCAGCACTGTAACTTCGCAGAAAAGAGTCGGATTA
CCAAAACACTGCCTGCTCTTCAGACTTAAAGCACTGATAGGACTTAAAATAGTCTCATTCAAATACTGTATTTTATATAGGCATTTCACA
AAAACAGCAAAATTGTGGCATTTTGTGAGGCCAAGGCTTGGATGCGTGTGTAATAGAGCCTTGTGGTGTGTGCGCACACACCCAGAGGGA
GAGTTTGAAAAATGCTTATTGGACACGTAACCTGGCTCTAATTTGGGCTGTTTTTCAGATACACTGTGATAAGTTCTTTTACAAATATCT
ATAGACATGGTAAACTTTTGGTTTTCAGATATGCTTAATGATAGTCTTACTAAATGCAGAAATAAGAATAAACTTTCTCAAATTATTAAA
AATGCCTACACAGTAAGTGTGAATTGCTGCAACAGGTTTGTTCTCAGGAGGGTAAGAACTCCAGGTCTAAACAGCTGACCCAGTGATGGG
GAATTTATCCTTGACCAATTTATCCTTGACCAATAACCTAATTGTCTATTCCTGAGTTATAAAAGTCCCCATCCTTATTAGCTCTACTGG
AATTTTCATACACGTAAATGCAGAAGTTACTAAGTATTAAGTATTACTGAGTATTAAGTAGTAATCTGTCAGTTATTAAAATTTGTAAAA
TCTATTTATGAAAGGTCATTAAACCAGATCATGTTCCTTTTTTTGTAATCAAGGTGACTAAGAAAATCAGTTGTGTAAATAAAATCATGT

>In-frame_ENST00000420658_ENST00000355710_X56348_AFAP1_chr4_7870348_-_RET_chr10_43612031_length(amino acids)=477AA_start in transcript=273_stop in transcript=1706
MEELIVELRLFLELLDHEYLTSTVREKKAVITNILLRIQSSKGFDVKDHAQKQETANSLPAPPQMPLPEIPQPWLEDPKWEFPRKNLVLG
KTLGEGEFGKVVKATAFHLKGRAGYTTVAVKMLKENASPSELRDLLSEFNVLKQVNHPHVIKLYGACSQDGPLLLIVEYAKYGSLRGFLR
ESRKVGPGYLGSGGSRNSSSLDHPDERALTMGDLISFAWQISQGMQYLAEMKLVHRDLAARNILVAEGRKMKISDFGLSRDVYEEDSYVK
RSQGRIPVKWMAIESLFDHIYTTQSDVWSFGVLLWEIVTLGGNPYPGIPPERLFNLLKTGHRMERPDNCSEEMYRLMLQCWKQEPDKRPV
FADISKDLEKMMVKRRDYLDLAASTPSDSLIYDDGLSEEETPLVDCNNAPLPRALPSTWIENKLYGMSDPNWPGESPVPLTRADGTNTGF

--------------------------------------------------------------
>In-frame_ENST00000420658_ENST00000340058_X56348_AFAP1_chr4_7870348_-_RET_chr10_43612031_length(transcript)=2331nt_BP=498nt
GGGCGCCCGGCTCCGCTGGGGGCGGGACGGCGGGCGGCGGGCGGGGCCGTGTGCGCCTGCGTGGTGTCTGTCCCGGCCGCAGCCCCACGC
CGGCTGCAGCTCCGCGAGCCGAGCCGGATCTCGAGCGGGGTACACGGGCTGAGGCGGCGGCGGCGGCCCCGCCCCGGGGCTCCATTGTTA
AGGCGGCTGCGGCTCTGTCGGCTGTTCAGCTGCGCTCCAGTCGGCCCAGGCGCCGCGGCGAGGAGCGAGCGCTGCGAGGTGGCGGCCTGA
GCAATGGAAGAGTTAATAGTTGAACTTCGTCTCTTTCTTGAACTCCTGGACCATGAATATCTAACCTCAACTGTCAGGGAGAAAAAGGCA
GTGATAACCAACATTCTGCTAAGAATACAGTCATCCAAAGGTTTTGATGTGAAGGACCATGCTCAGAAGCAGGAGACCGCTAACAGCCTG
CCAGCCCCTCCTCAGATGCCCCTGCCGGAGATCCCTCAGCCCTGGCTGGAGGATCCAAAGTGGGAATTCCCTCGGAAGAACTTGGTTCTT
GGAAAAACTCTAGGAGAAGGCGAATTTGGAAAAGTGGTCAAGGCAACGGCCTTCCATCTGAAAGGCAGAGCAGGGTACACCACGGTGGCC
GTGAAGATGCTGAAAGAGAACGCCTCCCCGAGTGAGCTGCGAGACCTGCTGTCAGAGTTCAACGTCCTGAAGCAGGTCAACCACCCACAT
GTCATCAAATTGTATGGGGCCTGCAGCCAGGATGGCCCGCTCCTCCTCATCGTGGAGTACGCCAAATACGGCTCCCTGCGGGGCTTCCTC
CGCGAGAGCCGCAAAGTGGGGCCTGGCTACCTGGGCAGTGGAGGCAGCCGCAACTCCAGCTCCCTGGACCACCCGGATGAGCGGGCCCTC
ACCATGGGCGACCTCATCTCATTTGCCTGGCAGATCTCACAGGGGATGCAGTATCTGGCCGAGATGAAGCTCGTTCATCGGGACTTGGCA
GCCAGAAACATCCTGGTAGCTGAGGGGCGGAAGATGAAGATTTCGGATTTCGGCTTGTCCCGAGATGTTTATGAAGAGGATTCCTACGTG
AAGAGGAGCCAGGGTCGGATTCCAGTTAAATGGATGGCAATTGAATCCCTTTTTGATCATATCTACACCACGCAAAGTGATGTATGGTCT
TTTGGTGTCCTGCTGTGGGAGATCGTGACCCTAGGGGGAAACCCCTATCCTGGGATTCCTCCTGAGCGGCTCTTCAACCTTCTGAAGACC
GGCCACCGGATGGAGAGGCCAGACAACTGCAGCGAGGAGATGTACCGCCTGATGCTGCAATGCTGGAAGCAGGAGCCGGACAAAAGGCCG
GTGTTTGCGGACATCAGCAAAGACCTGGAGAAGATGATGGTTAAGAGGAGAGACTACTTGGACCTTGCGGCGTCCACTCCATCTGACTCC
CTGATTTATGACGACGGCCTCTCAGAGGAGGAGACACCGCTGGTGGACTGTAATAATGCCCCCCTCCCTCGAGCCCTCCCTTCCACATGG
ATTGAAAACAAACTCTATGGTAGAATTTCCCATGCATTTACTAGATTCTAGCACCGCTGTCCCCTTTGCACTATCCTTCCTCTCTGTGAT
GCTTTTTAAAAATGTTTCTGGTCTGAACAAAACCAAAGTCTGCTCTGAACCTTTTTATTTGTAAATGTCTGACTTTGCATCCAGTTTACA
TTTAGGCATTATTGCAACTATGTTTTTCTAAAAGGATGTGAAAATAAGTGTAATTACCACATTGCCCAGCAACTTAGGATGGTAGAGGAA
AAAACAGATCAGGGCGGAACTCTCAGGGGAGACCAAGAACAGGTTGAATAAGGCGCTTCTGGGGTGGGAATCAAGTCATAGTACTTCTAC
TTTAACTAAGTGGATAAATATACAAATCTGGGGAGGTATTCAGTTGAGAAAGGAGCCACCAGCACCACTCAGCCTGCACTGGGAGCACAG
CCAGGTTCCCCCAGACCCCTCCTGGGCAGGCAGGTGCCTCTCAGAGGCCACCCGGCACTGGCGAGCAGCCACTGGCCAAGCCTCAGCCCC
AGTCCCAGCCACATGTCCTCCATCAGGGGTAGCGAGGTTGCAGGAGCTGGCTGGCCCTGGGAGGACGCACCCCCACTGCTGTTTTCACAT
CCTTTCCCTTACCCACCTTCAGGACGGTTGTCACTTATGAAGTCAGTGCTAAAGCTGGAGCAGTTGCTTTTTGAAAGAACATGGTCTGTG

>In-frame_ENST00000420658_ENST00000340058_X56348_AFAP1_chr4_7870348_-_RET_chr10_43612031_length(amino acids)=435AA_start in transcript=273_stop in transcript=1580
MEELIVELRLFLELLDHEYLTSTVREKKAVITNILLRIQSSKGFDVKDHAQKQETANSLPAPPQMPLPEIPQPWLEDPKWEFPRKNLVLG
KTLGEGEFGKVVKATAFHLKGRAGYTTVAVKMLKENASPSELRDLLSEFNVLKQVNHPHVIKLYGACSQDGPLLLIVEYAKYGSLRGFLR
ESRKVGPGYLGSGGSRNSSSLDHPDERALTMGDLISFAWQISQGMQYLAEMKLVHRDLAARNILVAEGRKMKISDFGLSRDVYEEDSYVK
RSQGRIPVKWMAIESLFDHIYTTQSDVWSFGVLLWEIVTLGGNPYPGIPPERLFNLLKTGHRMERPDNCSEEMYRLMLQCWKQEPDKRPV

--------------------------------------------------------------
>In-frame_ENST00000382543_ENST00000355710_X56348_AFAP1_chr4_7870348_-_RET_chr10_43612031_length(transcript)=3520nt_BP=229nt
AGCAATGGAAGAGTTAATAGTTGAACTTCGTCTCTTTCTTGAACTCCTGGACCATGAATATCTAACCTCAACTGTCAGGGAGAAAAAGGC
AGTGATAACCAACATTCTGCTAAGAATACAGTCATCCAAAGGTTTTGATGTGAAGGACCATGCTCAGAAGCAGGAGACCGCTAACAGCCT
GCCAGCCCCTCCTCAGATGCCCCTGCCGGAGATCCCTCAGCCCTGGCTGGAGGATCCAAAGTGGGAATTCCCTCGGAAGAACTTGGTTCT
TGGAAAAACTCTAGGAGAAGGCGAATTTGGAAAAGTGGTCAAGGCAACGGCCTTCCATCTGAAAGGCAGAGCAGGGTACACCACGGTGGC
CGTGAAGATGCTGAAAGAGAACGCCTCCCCGAGTGAGCTGCGAGACCTGCTGTCAGAGTTCAACGTCCTGAAGCAGGTCAACCACCCACA
TGTCATCAAATTGTATGGGGCCTGCAGCCAGGATGGCCCGCTCCTCCTCATCGTGGAGTACGCCAAATACGGCTCCCTGCGGGGCTTCCT
CCGCGAGAGCCGCAAAGTGGGGCCTGGCTACCTGGGCAGTGGAGGCAGCCGCAACTCCAGCTCCCTGGACCACCCGGATGAGCGGGCCCT
CACCATGGGCGACCTCATCTCATTTGCCTGGCAGATCTCACAGGGGATGCAGTATCTGGCCGAGATGAAGCTCGTTCATCGGGACTTGGC
AGCCAGAAACATCCTGGTAGCTGAGGGGCGGAAGATGAAGATTTCGGATTTCGGCTTGTCCCGAGATGTTTATGAAGAGGATTCCTACGT
GAAGAGGAGCCAGGGTCGGATTCCAGTTAAATGGATGGCAATTGAATCCCTTTTTGATCATATCTACACCACGCAAAGTGATGTATGGTC
TTTTGGTGTCCTGCTGTGGGAGATCGTGACCCTAGGGGGAAACCCCTATCCTGGGATTCCTCCTGAGCGGCTCTTCAACCTTCTGAAGAC
CGGCCACCGGATGGAGAGGCCAGACAACTGCAGCGAGGAGATGTACCGCCTGATGCTGCAATGCTGGAAGCAGGAGCCGGACAAAAGGCC
GGTGTTTGCGGACATCAGCAAAGACCTGGAGAAGATGATGGTTAAGAGGAGAGACTACTTGGACCTTGCGGCGTCCACTCCATCTGACTC
CCTGATTTATGACGACGGCCTCTCAGAGGAGGAGACACCGCTGGTGGACTGTAATAATGCCCCCCTCCCTCGAGCCCTCCCTTCCACATG
GATTGAAAACAAACTCTATGGCATGTCAGACCCGAACTGGCCTGGAGAGAGTCCTGTACCACTCACGAGAGCTGATGGCACTAACACTGG
GTTTCCAAGATATCCAAATGATAGTGTATATGCTAACTGGATGCTTTCACCCTCAGCGGCAAAATTAATGGACACGTTTGATAGTTAACA
TTTCTTTGTGAAAGGTAATGGACTCACAAGGGGAAGAAACATGCTGAGAATGGAAAGTCTACCGGCCCTTTCTTTGTGAACGTCACATTG
GCCGAGCCGTGTTCAGTTCCCAGGTGGCAGACTCGTTTTTGGTAGTTTGTTTTAACTTCCAAGGTGGTTTTACTTCTGATAGCCGGTGAT
TTTCCCTCCTAGCAGACATGCCACACCGGGTAAGAGCTCTGAGTCTTAGTGGTTAAGCATTCCTTTCTCTTCAGTGCCCAGCAGCACCCA
GTGTTGGTCTGTGTCCATCAGTGACCACCAACATTCTGTGTTCACATGTGTGGGTCCAACACTTACTACCTGGTGTATGAAATTGGACCT
GAACTGTTGGATTTTTCTAGTTGCCGCCAAACAAGGCAAAAAAATTTAAACATGAAGCACACACACAAAAAAGGCAGTAGGAAAAATGCT
GGCCCTGATGACCTGTCCTTATTCAGAATGAGAGACTGCGGGGGGGGCCTGGGGGTAGTGTCAATGCCCCTCCAGGGCTGGAGGGGAAGA
GGGGCCCCGAGGATGGGCCTGGGCTCAGCATTCGAGATCTTGAGAATGATTTTTTTTAAATCATGCAACCTTTCCTTAGGAAGACATTTG
GTTTTCATCATGATTAAGATGATTCCTAGATTTAGCACAATGGAGAGATTCCATGCCATCTTTACTATGTGGATGGTGGTATCAGGGAAG
AGGGCTCACAAGACACATTTGTCCCCCGGGCCCACCACATCATCCTCACGTGTTCGGTACTGAGCAGCCACTACCCCTGATGAGAACAGT
ATGAAGAAAGGGGGCTGTTGGAGTCCCAGAATTGCTGACAGCAGAGGCTTTGCTGCTGTGAATCCCACCTGCCACCAGCCTGCAGCACAC
CCCACAGCCAAGTAGAGGCGAAAGCAGTGGCTCATCCTACCTGTTAGGAGCAGGTAGGGCTTGTACTCACTTTAATTTGAATCTTATCAA
CTTACTCATAAAGGGACAGGCTAGCTAGCTGTGTTAGAAGTAGCAATGACAATGACCAAGGACTGCTACACCTCTGATTACAATTCTGAT
GTGAAAAAGATGGTGTTTGGCTCTTATAGAGCCTGTGTGAAAGGCCCATGGATCAGCTCTTCCTGTGTTTGTAATTTAATGCTGCTACAA
GATGTTTCTGTTTCTTAGATTCTGACCATGACTCATAAGCTTCTTGTCATTCTTCATTGCTTGTTTGTGGTCACAGATGCACAACACTCC
TCCAGTCTTGTGGGGGCAGCTTTTGGGAAGTCTCAGCAGCTCTTCTGGCTGTGTTGTCAGCACTGTAACTTCGCAGAAAAGAGTCGGATT
ACCAAAACACTGCCTGCTCTTCAGACTTAAAGCACTGATAGGACTTAAAATAGTCTCATTCAAATACTGTATTTTATATAGGCATTTCAC
AAAAACAGCAAAATTGTGGCATTTTGTGAGGCCAAGGCTTGGATGCGTGTGTAATAGAGCCTTGTGGTGTGTGCGCACACACCCAGAGGG
AGAGTTTGAAAAATGCTTATTGGACACGTAACCTGGCTCTAATTTGGGCTGTTTTTCAGATACACTGTGATAAGTTCTTTTACAAATATC
TATAGACATGGTAAACTTTTGGTTTTCAGATATGCTTAATGATAGTCTTACTAAATGCAGAAATAAGAATAAACTTTCTCAAATTATTAA
AAATGCCTACACAGTAAGTGTGAATTGCTGCAACAGGTTTGTTCTCAGGAGGGTAAGAACTCCAGGTCTAAACAGCTGACCCAGTGATGG
GGAATTTATCCTTGACCAATTTATCCTTGACCAATAACCTAATTGTCTATTCCTGAGTTATAAAAGTCCCCATCCTTATTAGCTCTACTG
GAATTTTCATACACGTAAATGCAGAAGTTACTAAGTATTAAGTATTACTGAGTATTAAGTAGTAATCTGTCAGTTATTAAAATTTGTAAA
ATCTATTTATGAAAGGTCATTAAACCAGATCATGTTCCTTTTTTTGTAATCAAGGTGACTAAGAAAATCAGTTGTGTAAATAAAATCATG

>In-frame_ENST00000382543_ENST00000355710_X56348_AFAP1_chr4_7870348_-_RET_chr10_43612031_length(amino acids)=477AA_start in transcript=4_stop in transcript=1437
MEELIVELRLFLELLDHEYLTSTVREKKAVITNILLRIQSSKGFDVKDHAQKQETANSLPAPPQMPLPEIPQPWLEDPKWEFPRKNLVLG
KTLGEGEFGKVVKATAFHLKGRAGYTTVAVKMLKENASPSELRDLLSEFNVLKQVNHPHVIKLYGACSQDGPLLLIVEYAKYGSLRGFLR
ESRKVGPGYLGSGGSRNSSSLDHPDERALTMGDLISFAWQISQGMQYLAEMKLVHRDLAARNILVAEGRKMKISDFGLSRDVYEEDSYVK
RSQGRIPVKWMAIESLFDHIYTTQSDVWSFGVLLWEIVTLGGNPYPGIPPERLFNLLKTGHRMERPDNCSEEMYRLMLQCWKQEPDKRPV
FADISKDLEKMMVKRRDYLDLAASTPSDSLIYDDGLSEEETPLVDCNNAPLPRALPSTWIENKLYGMSDPNWPGESPVPLTRADGTNTGF

--------------------------------------------------------------
>In-frame_ENST00000382543_ENST00000340058_X56348_AFAP1_chr4_7870348_-_RET_chr10_43612031_length(transcript)=2062nt_BP=229nt
AGCAATGGAAGAGTTAATAGTTGAACTTCGTCTCTTTCTTGAACTCCTGGACCATGAATATCTAACCTCAACTGTCAGGGAGAAAAAGGC
AGTGATAACCAACATTCTGCTAAGAATACAGTCATCCAAAGGTTTTGATGTGAAGGACCATGCTCAGAAGCAGGAGACCGCTAACAGCCT
GCCAGCCCCTCCTCAGATGCCCCTGCCGGAGATCCCTCAGCCCTGGCTGGAGGATCCAAAGTGGGAATTCCCTCGGAAGAACTTGGTTCT
TGGAAAAACTCTAGGAGAAGGCGAATTTGGAAAAGTGGTCAAGGCAACGGCCTTCCATCTGAAAGGCAGAGCAGGGTACACCACGGTGGC
CGTGAAGATGCTGAAAGAGAACGCCTCCCCGAGTGAGCTGCGAGACCTGCTGTCAGAGTTCAACGTCCTGAAGCAGGTCAACCACCCACA
TGTCATCAAATTGTATGGGGCCTGCAGCCAGGATGGCCCGCTCCTCCTCATCGTGGAGTACGCCAAATACGGCTCCCTGCGGGGCTTCCT
CCGCGAGAGCCGCAAAGTGGGGCCTGGCTACCTGGGCAGTGGAGGCAGCCGCAACTCCAGCTCCCTGGACCACCCGGATGAGCGGGCCCT
CACCATGGGCGACCTCATCTCATTTGCCTGGCAGATCTCACAGGGGATGCAGTATCTGGCCGAGATGAAGCTCGTTCATCGGGACTTGGC
AGCCAGAAACATCCTGGTAGCTGAGGGGCGGAAGATGAAGATTTCGGATTTCGGCTTGTCCCGAGATGTTTATGAAGAGGATTCCTACGT
GAAGAGGAGCCAGGGTCGGATTCCAGTTAAATGGATGGCAATTGAATCCCTTTTTGATCATATCTACACCACGCAAAGTGATGTATGGTC
TTTTGGTGTCCTGCTGTGGGAGATCGTGACCCTAGGGGGAAACCCCTATCCTGGGATTCCTCCTGAGCGGCTCTTCAACCTTCTGAAGAC
CGGCCACCGGATGGAGAGGCCAGACAACTGCAGCGAGGAGATGTACCGCCTGATGCTGCAATGCTGGAAGCAGGAGCCGGACAAAAGGCC
GGTGTTTGCGGACATCAGCAAAGACCTGGAGAAGATGATGGTTAAGAGGAGAGACTACTTGGACCTTGCGGCGTCCACTCCATCTGACTC
CCTGATTTATGACGACGGCCTCTCAGAGGAGGAGACACCGCTGGTGGACTGTAATAATGCCCCCCTCCCTCGAGCCCTCCCTTCCACATG
GATTGAAAACAAACTCTATGGTAGAATTTCCCATGCATTTACTAGATTCTAGCACCGCTGTCCCCTTTGCACTATCCTTCCTCTCTGTGA
TGCTTTTTAAAAATGTTTCTGGTCTGAACAAAACCAAAGTCTGCTCTGAACCTTTTTATTTGTAAATGTCTGACTTTGCATCCAGTTTAC
ATTTAGGCATTATTGCAACTATGTTTTTCTAAAAGGATGTGAAAATAAGTGTAATTACCACATTGCCCAGCAACTTAGGATGGTAGAGGA
AAAAACAGATCAGGGCGGAACTCTCAGGGGAGACCAAGAACAGGTTGAATAAGGCGCTTCTGGGGTGGGAATCAAGTCATAGTACTTCTA
CTTTAACTAAGTGGATAAATATACAAATCTGGGGAGGTATTCAGTTGAGAAAGGAGCCACCAGCACCACTCAGCCTGCACTGGGAGCACA
GCCAGGTTCCCCCAGACCCCTCCTGGGCAGGCAGGTGCCTCTCAGAGGCCACCCGGCACTGGCGAGCAGCCACTGGCCAAGCCTCAGCCC
CAGTCCCAGCCACATGTCCTCCATCAGGGGTAGCGAGGTTGCAGGAGCTGGCTGGCCCTGGGAGGACGCACCCCCACTGCTGTTTTCACA
TCCTTTCCCTTACCCACCTTCAGGACGGTTGTCACTTATGAAGTCAGTGCTAAAGCTGGAGCAGTTGCTTTTTGAAAGAACATGGTCTGT

>In-frame_ENST00000382543_ENST00000340058_X56348_AFAP1_chr4_7870348_-_RET_chr10_43612031_length(amino acids)=435AA_start in transcript=4_stop in transcript=1311
MEELIVELRLFLELLDHEYLTSTVREKKAVITNILLRIQSSKGFDVKDHAQKQETANSLPAPPQMPLPEIPQPWLEDPKWEFPRKNLVLG
KTLGEGEFGKVVKATAFHLKGRAGYTTVAVKMLKENASPSELRDLLSEFNVLKQVNHPHVIKLYGACSQDGPLLLIVEYAKYGSLRGFLR
ESRKVGPGYLGSGGSRNSSSLDHPDERALTMGDLISFAWQISQGMQYLAEMKLVHRDLAARNILVAEGRKMKISDFGLSRDVYEEDSYVK
RSQGRIPVKWMAIESLFDHIYTTQSDVWSFGVLLWEIVTLGGNPYPGIPPERLFNLLKTGHRMERPDNCSEEMYRLMLQCWKQEPDKRPV

--------------------------------------------------------------

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Fusion Gene PPI Analysis for AFAP1-RET


check button Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in

ChiPPI page.


check button Protein-protein interactors with each fusion partner protein in wild-type (BIOGRID-3.4.160)
HgeneHgene's interactorsTgeneTgene's interactors


check button - Retained PPIs in in-frame fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenStill interaction with


check button - Lost PPIs in in-frame fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenInteraction lost with
HgeneAFAP1chr4:7870348chr10:43612031ENST00000358461-317594_63775.0731.0F-actin
HgeneAFAP1chr4:7870348chr10:43612031ENST00000360265-216594_63775.0731.0F-actin
HgeneAFAP1chr4:7870348chr10:43612031ENST00000382543-217594_63775.0815.0F-actin
HgeneAFAP1chr4:7870348chr10:43612031ENST00000420658-318594_63775.0815.0F-actin


check button - Retained PPIs, but lost function due to frame-shift fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenInteraction lost with


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Related Drugs for AFAP1-RET


check button Drugs targeting genes involved in this fusion gene.
(DrugBank Version 5.1.8 2021-05-08)
PartnerGeneUniProtAccDrugBank IDDrug nameDrug activityDrug typeDrug status

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Related Diseases for AFAP1-RET


check button Diseases associated with fusion partners.
(DisGeNet 4.0)
PartnerGeneDisease IDDisease name# pubmedsSource
TgeneRETC1833921Familial medullary thyroid carcinoma23CTD_human;GENOMICS_ENGLAND;ORPHANET;UNIPROT
TgeneRETC3888239HIRSCHSPRUNG DISEASE, SUSCEPTIBILITY TO, 116GENOMICS_ENGLAND;UNIPROT
TgeneRETC0025268Multiple Endocrine Neoplasia Type 2a15CTD_human;GENOMICS_ENGLAND;ORPHANET;UNIPROT
TgeneRETC1708353Hereditary Paraganglioma-Pheochromocytoma Syndrome12CLINGEN
TgeneRETC0025269Multiple Endocrine Neoplasia Type 2b10CTD_human;GENOMICS_ENGLAND;ORPHANET;UNIPROT
TgeneRETC0238463Papillary thyroid carcinoma3CTD_human;ORPHANET
TgeneRETC1275808Congenital central hypoventilation3CTD_human;GENOMICS_ENGLAND;UNIPROT
TgeneRETC1859049CCHS WITH HIRSCHSPRUNG DISEASE3CTD_human;ORPHANET
TgeneRETC0009402Colorectal Carcinoma2CTD_human;UNIPROT
TgeneRETC0009404Colorectal Neoplasms2CTD_human
TgeneRETC0019569Hirschsprung Disease2CTD_human
TgeneRETC0027662Multiple Endocrine Neoplasia2CTD_human;GENOMICS_ENGLAND
TgeneRETC0085758Aganglionosis, Colonic2CTD_human
TgeneRETC0266294Unilateral agenesis of kidney2ORPHANET
TgeneRETC1257840Aganglionosis, Rectosigmoid Colon2CTD_human
TgeneRETC3661523Congenital Intestinal Aganglionosis2CTD_human
TgeneRETC0006413Burkitt Lymphoma1CTD_human
TgeneRETC0031511Pheochromocytoma1CGI;CTD_human;GENOMICS_ENGLAND;UNIPROT
TgeneRETC0038220Status Epilepticus1CTD_human
TgeneRETC0040136Thyroid Neoplasm1CGI;CTD_human
TgeneRETC0151468Thyroid Gland Follicular Adenoma1CTD_human
TgeneRETC0206693Medullary carcinoma1CTD_human
TgeneRETC0238462Medullary carcinoma of thyroid1CGI;CTD_human
TgeneRETC0270823Petit mal status1CTD_human
TgeneRETC0311335Grand Mal Status Epilepticus1CTD_human
TgeneRETC0343640African Burkitt's lymphoma1CTD_human
TgeneRETC0393734Complex Partial Status Epilepticus1CTD_human
TgeneRETC0549473Thyroid carcinoma1CGI;CTD_human;UNIPROT
TgeneRETC0740340Amyloidosis, Familial1CTD_human
TgeneRETC0751522Status Epilepticus, Subclinical1CTD_human
TgeneRETC0751523Non-Convulsive Status Epilepticus1CTD_human
TgeneRETC0751524Simple Partial Status Epilepticus1CTD_human
TgeneRETC1257877Pheochromocytoma, Extra-Adrenal1CTD_human
TgeneRETC1609433Congenital absence of kidneys syndrome1CTD_human;GENOMICS_ENGLAND;ORPHANET
TgeneRETC3501843Nonmedullary Thyroid Carcinoma1CTD_human
TgeneRETC3501844Familial Nonmedullary Thyroid Cancer1CTD_human
TgeneRETC4721444Burkitt Leukemia1CTD_human