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Center for Computational Systems Medicine
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Fusion Gene Summary

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Fusion Gene ORF analysis

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Fusion Genomic Features

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Fusion Protein Features

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Fusion Gene Sequence

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Fusion Gene PPI analysis

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Related Drugs

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Related Diseases

Fusion gene:EZH1-TOP2A (FusionGDB2 ID:28016)

Fusion Gene Summary for EZH1-TOP2A

check button Fusion gene summary
Fusion gene informationFusion gene name: EZH1-TOP2A
Fusion gene ID: 28016
HgeneTgene
Gene symbol

EZH1

TOP2A

Gene ID

2145

7153

Gene nameenhancer of zeste 1 polycomb repressive complex 2 subunitDNA topoisomerase II alpha
SynonymsKMT6BTOP2|TP2A
Cytomap

17q21.2

17q21.2

Type of geneprotein-codingprotein-coding
Descriptionhistone-lysine N-methyltransferase EZH1ENX-2enhancer of zeste homolog 1DNA topoisomerase 2-alphaDNA gyraseDNA topoisomerase (ATP-hydrolyzing)DNA topoisomerase II, 170 kDDNA topoisomerase II, alpha isozymetopoisomerase (DNA) II alpha 170kDa
Modification date2020031520200322
UniProtAcc

Q92800

.
Ensembl transtripts involved in fusion geneENST00000428826, ENST00000415827, 
ENST00000435174, ENST00000592743, 
ENST00000585893, ENST00000590078, 
ENST00000590783, 		ENST00000423485, 
Fusion gene scores* DoF score8 X 12 X 6=5768 X 9 X 4=288
# samples 1212
** MAII scorelog2(12/576*10)=-2.26303440583379
possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs).
DoF>8 and MAII<0		log2(12/288*10)=-1.26303440583379
possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs).
DoF>8 and MAII<0
Context

PubMed: EZH1 [Title/Abstract] AND TOP2A [Title/Abstract] AND fusion [Title/Abstract]

Most frequent breakpointEZH1(40854895)-TOP2A(38561135), # samples:3
Anticipated loss of major functional domain due to fusion event.EZH1-TOP2A seems lost the major protein functional domain in Hgene partner, which is a tumor suppressor due to the frame-shifted ORF.
EZH1-TOP2A seems lost the major protein functional domain in Hgene partner, which is a IUPHAR drug target due to the frame-shifted ORF.
EZH1-TOP2A seems lost the major protein functional domain in Hgene partner, which is a epigenetic factor due to the frame-shifted ORF.
EZH1-TOP2A seems lost the major protein functional domain in Tgene partner, which is a essential gene due to the frame-shifted ORF.
EZH1-TOP2A seems lost the major protein functional domain in Tgene partner, which is a IUPHAR drug target due to the frame-shifted ORF.
EZH1-TOP2A seems lost the major protein functional domain in Tgene partner, which is a epigenetic factor due to the frame-shifted ORF.
* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

check button Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez
PartnerGeneGO IDGO termPubMed ID
TgeneTOP2A

GO:0006265

DNA topological change

22323612

TgeneTOP2A

GO:0006266

DNA ligation

15491148

TgeneTOP2A

GO:0006974

cellular response to DNA damage stimulus

16611985

TgeneTOP2A

GO:0030263

apoptotic chromosome condensation

10959840

TgeneTOP2A

GO:0043065

positive regulation of apoptotic process

16611985


check buttonFusion gene breakpoints across EZH1 (5'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

check buttonFusion gene breakpoints across TOP2A (3'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

check button Fusion gene information from two resources (ChiTars 5.0 and ChimerDB 4.0)
* All genome coordinats were lifted-over on hg19.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.
SourceDiseaseSampleHgeneHchrHbpHstrandTgeneTchrTbpTstrand
ChimerDB4BRCATCGA-B6-A0IG-01AEZH1chr17

40854895

-TOP2Achr17

38561135

-
ChimerDB4BRCATCGA-B6-A0IGEZH1chr17

40854894

-TOP2Achr17

38561135

-
ChimerDB4BRCATCGA-B6-A0IG-01AEZH1chr17

40854895

-TOP2Achr17

38561135

-
ChimerDB4BRCATCGA-B6-A0IG-01AEZH1chr17

40854895

-TOP2Achr17

38561135

-


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Fusion Gene ORF analysis for EZH1-TOP2A

check button Open reading frame (ORF) analsis of fusion genes based on Ensembl gene isoform structure.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.
ORFHenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrand
Frame-shiftENST00000428826ENST00000423485EZH1chr17

40854895

-TOP2Achr17

38561135

-
Frame-shiftENST00000415827ENST00000423485EZH1chr17

40854895

-TOP2Achr17

38561135

-
Frame-shiftENST00000435174ENST00000423485EZH1chr17

40854895

-TOP2Achr17

38561135

-
In-frameENST00000592743ENST00000423485EZH1chr17

40854895

-TOP2Achr17

38561135

-
Frame-shiftENST00000585893ENST00000423485EZH1chr17

40854895

-TOP2Achr17

38561135

-
In-frameENST00000590078ENST00000423485EZH1chr17

40854895

-TOP2Achr17

38561135

-
intron-3CDSENST00000590783ENST00000423485EZH1chr17

40854895

-TOP2Achr17

38561135

-
Frame-shiftENST00000428826ENST00000423485EZH1chr17

40854894

-TOP2Achr17

38561135

-
Frame-shiftENST00000415827ENST00000423485EZH1chr17

40854894

-TOP2Achr17

38561135

-
Frame-shiftENST00000435174ENST00000423485EZH1chr17

40854894

-TOP2Achr17

38561135

-
In-frameENST00000592743ENST00000423485EZH1chr17

40854894

-TOP2Achr17

38561135

-
Frame-shiftENST00000585893ENST00000423485EZH1chr17

40854894

-TOP2Achr17

38561135

-
In-frameENST00000590078ENST00000423485EZH1chr17

40854894

-TOP2Achr17

38561135

-
intron-3CDSENST00000590783ENST00000423485EZH1chr17

40854894

-TOP2Achr17

38561135

-

check buttonORFfinder result based on the fusion transcript sequence of in-frame fusion genes.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandSeq length
(transcript)		BP loci
(transcript)		Predicted start
(transcript)		Predicted stop
(transcript)		Seq length
(amino acids)
ENST00000592743EZH1chr1740854895-ENST00000423485TOP2Achr1738561135-5879223322844875863
ENST00000590078EZH1chr1740854895-ENST00000423485TOP2Achr1738561135-5830218422354826863
ENST00000592743EZH1chr1740854894-ENST00000423485TOP2Achr1738561135-5879223322844875863
ENST00000590078EZH1chr1740854894-ENST00000423485TOP2Achr1738561135-5830218422354826863

check buttonDeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandNo-coding scoreCoding score
ENST00000592743ENST00000423485EZH1chr1740854895-TOP2Achr1738561135-0.0011144090.9988856
ENST00000590078ENST00000423485EZH1chr1740854895-TOP2Achr1738561135-0.0010189530.99898106
ENST00000592743ENST00000423485EZH1chr1740854894-TOP2Achr1738561135-0.0011144090.9988856
ENST00000590078ENST00000423485EZH1chr1740854894-TOP2Achr1738561135-0.0010189530.99898106

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Fusion Genomic Features for EZH1-TOP2A


check buttonFusionAI prediction of the potential fusion gene breakpoint based on the pre-mature RNA sequence context (+/- 5kb of individual partner genes, total 20kb length sequence). FusionAI is a fusion gene breakpoint classifier based on convolutional neural network by comparing the fusion positive and negative sequence context of ~ 20K fusion gene data. From here, we can have the relative potentency of the 20K genomic sequence how individual sequnce will be likely used as the gene fusion breakpoints.
HgeneHchrHbpHstrandTgeneTchrTbpTstrand1-pp (fusion gene breakpoint)

check buttonDistribution of 44 human genomic features loci across 20kb length fusion breakpoint regions. We integrated a total of 44 different types of human genomic feature loci information across five big categories including virus integration sites, repeats, structural variants, chromatin states, and gene expression regulation. More details are in help page.

check buttonDistribution of 44 human genomic features loci across 20kb length fusion breakpoint regions that are ovelapped with the top 1% feature importance score regions. More details are in help page.

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Fusion Protein Features for EZH1-TOP2A


check button Go to

FGviewer for the breakpoints of chr17:40854895-chr17:38561135

.
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
FGviewer

check buttonMain function of each fusion partner protein. (from UniProt)
HgeneTgene
EZH1

Q92800

.
FUNCTION: Polycomb group (PcG) protein. Catalytic subunit of the PRC2/EED-EZH1 complex, which methylates 'Lys-27' of histone H3, leading to transcriptional repression of the affected target gene. Able to mono-, di- and trimethylate 'Lys-27' of histone H3 to form H3K27me1, H3K27me2 and H3K27me3, respectively. Required for embryonic stem cell derivation and self-renewal, suggesting that it is involved in safeguarding embryonic stem cell identity. Compared to EZH2-containing complexes, it is less abundant in embryonic stem cells, has weak methyltransferase activity and plays a less critical role in forming H3K27me3, which is required for embryonic stem cell identity and proper differentiation. {ECO:0000269|PubMed:19026781}.FUNCTION: Might normally function as a transcriptional repressor. EWS-fusion-proteins (EFPS) may play a role in the tumorigenic process. They may disturb gene expression by mimicking, or interfering with the normal function of CTD-POLII within the transcription initiation complex. They may also contribute to an aberrant activation of the fusion protein target genes.

check buttonRetention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at

download page


* Minus value of BPloci means that the break pointn is located before the CDS.
- In-frame and retained protein feature among the 13 regional features.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note
HgeneEZH1chr17:40854894chr17:38561135ENST00000428826-2021524_606727.6666666666666748.0Compositional biasNote=Cys-rich
HgeneEZH1chr17:40854894chr17:38561135ENST00000585893-1920524_606687.6666666666666708.0Compositional biasNote=Cys-rich
HgeneEZH1chr17:40854894chr17:38561135ENST00000590078-1920524_606657.6666666666666678.0Compositional biasNote=Cys-rich
HgeneEZH1chr17:40854894chr17:38561135ENST00000592743-1920524_606727.6666666666666748.0Compositional biasNote=Cys-rich
HgeneEZH1chr17:40854895chr17:38561135ENST00000428826-2021524_606727.6666666666666748.0Compositional biasNote=Cys-rich
HgeneEZH1chr17:40854895chr17:38561135ENST00000585893-1920524_606687.6666666666666708.0Compositional biasNote=Cys-rich
HgeneEZH1chr17:40854895chr17:38561135ENST00000590078-1920524_606657.6666666666666678.0Compositional biasNote=Cys-rich
HgeneEZH1chr17:40854895chr17:38561135ENST00000592743-1920524_606727.6666666666666748.0Compositional biasNote=Cys-rich
HgeneEZH1chr17:40854894chr17:38561135ENST00000428826-2021504_606727.6666666666666748.0DomainCXC
HgeneEZH1chr17:40854894chr17:38561135ENST00000428826-2021613_728727.6666666666666748.0DomainSET
HgeneEZH1chr17:40854894chr17:38561135ENST00000585893-1920504_606687.6666666666666708.0DomainCXC
HgeneEZH1chr17:40854894chr17:38561135ENST00000590078-1920504_606657.6666666666666678.0DomainCXC
HgeneEZH1chr17:40854894chr17:38561135ENST00000592743-1920504_606727.6666666666666748.0DomainCXC
HgeneEZH1chr17:40854894chr17:38561135ENST00000592743-1920613_728727.6666666666666748.0DomainSET
HgeneEZH1chr17:40854895chr17:38561135ENST00000428826-2021504_606727.6666666666666748.0DomainCXC
HgeneEZH1chr17:40854895chr17:38561135ENST00000428826-2021613_728727.6666666666666748.0DomainSET
HgeneEZH1chr17:40854895chr17:38561135ENST00000585893-1920504_606687.6666666666666708.0DomainCXC
HgeneEZH1chr17:40854895chr17:38561135ENST00000590078-1920504_606657.6666666666666678.0DomainCXC
HgeneEZH1chr17:40854895chr17:38561135ENST00000592743-1920504_606727.6666666666666748.0DomainCXC
HgeneEZH1chr17:40854895chr17:38561135ENST00000592743-1920613_728727.6666666666666748.0DomainSET
HgeneEZH1chr17:40854894chr17:38561135ENST00000428826-2021491_496727.6666666666666748.0MotifNuclear localization signal
HgeneEZH1chr17:40854894chr17:38561135ENST00000435174-1819491_496588.6666666666666609.0MotifNuclear localization signal
HgeneEZH1chr17:40854894chr17:38561135ENST00000585893-1920491_496687.6666666666666708.0MotifNuclear localization signal
HgeneEZH1chr17:40854894chr17:38561135ENST00000590078-1920491_496657.6666666666666678.0MotifNuclear localization signal
HgeneEZH1chr17:40854894chr17:38561135ENST00000592743-1920491_496727.6666666666666748.0MotifNuclear localization signal
HgeneEZH1chr17:40854895chr17:38561135ENST00000428826-2021491_496727.6666666666666748.0MotifNuclear localization signal
HgeneEZH1chr17:40854895chr17:38561135ENST00000435174-1819491_496588.6666666666666609.0MotifNuclear localization signal
HgeneEZH1chr17:40854895chr17:38561135ENST00000585893-1920491_496687.6666666666666708.0MotifNuclear localization signal
HgeneEZH1chr17:40854895chr17:38561135ENST00000590078-1920491_496657.6666666666666678.0MotifNuclear localization signal
HgeneEZH1chr17:40854895chr17:38561135ENST00000592743-1920491_496727.6666666666666748.0MotifNuclear localization signal
TgeneTOP2Achr17:40854894chr17:38561135ENST0000042348515351018_1028651.01532.0MotifNote=Nuclear export signal
TgeneTOP2Achr17:40854895chr17:38561135ENST0000042348515351018_1028651.01532.0MotifNote=Nuclear export signal

- In-frame and not-retained protein feature among the 13 regional features.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note
HgeneEZH1chr17:40854894chr17:38561135ENST00000435174-1819524_606588.6666666666666609.0Compositional biasNote=Cys-rich
HgeneEZH1chr17:40854895chr17:38561135ENST00000435174-1819524_606588.6666666666666609.0Compositional biasNote=Cys-rich
HgeneEZH1chr17:40854894chr17:38561135ENST00000435174-1819504_606588.6666666666666609.0DomainCXC
HgeneEZH1chr17:40854894chr17:38561135ENST00000435174-1819613_728588.6666666666666609.0DomainSET
HgeneEZH1chr17:40854894chr17:38561135ENST00000585893-1920613_728687.6666666666666708.0DomainSET
HgeneEZH1chr17:40854894chr17:38561135ENST00000590078-1920613_728657.6666666666666678.0DomainSET
HgeneEZH1chr17:40854895chr17:38561135ENST00000435174-1819504_606588.6666666666666609.0DomainCXC
HgeneEZH1chr17:40854895chr17:38561135ENST00000435174-1819613_728588.6666666666666609.0DomainSET
HgeneEZH1chr17:40854895chr17:38561135ENST00000585893-1920613_728687.6666666666666708.0DomainSET
HgeneEZH1chr17:40854895chr17:38561135ENST00000590078-1920613_728657.6666666666666678.0DomainSET
TgeneTOP2Achr17:40854894chr17:38561135ENST000004234851535455_572651.01532.0DomainToprim
TgeneTOP2Achr17:40854895chr17:38561135ENST000004234851535455_572651.01532.0DomainToprim
TgeneTOP2Achr17:40854894chr17:38561135ENST000004234851535148_150651.01532.0Nucleotide bindingATP
TgeneTOP2Achr17:40854894chr17:38561135ENST000004234851535161_168651.01532.0Nucleotide bindingATP
TgeneTOP2Achr17:40854894chr17:38561135ENST000004234851535376_378651.01532.0Nucleotide bindingATP
TgeneTOP2Achr17:40854895chr17:38561135ENST000004234851535148_150651.01532.0Nucleotide bindingATP
TgeneTOP2Achr17:40854895chr17:38561135ENST000004234851535161_168651.01532.0Nucleotide bindingATP
TgeneTOP2Achr17:40854895chr17:38561135ENST000004234851535376_378651.01532.0Nucleotide bindingATP


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Fusion Gene Sequence for EZH1-TOP2A


check button For in-frame fusion transcripts, we provide the fusion transcript sequences and fusion amino acid sequences. To have fusion amino acid sequence, we ran ORFfinder and chose the longest ORF among the all predicted ones.
>In-frame_ENST00000592743_ENST00000423485_TCGA-B6-A0IG-01A_EZH1_chr17_40854895_-_TOP2A_chr17_38561135_length(transcript)=5879nt_BP=2233nt
ATGCGTCCTAGCAGCGGGACCCGCGGCTCGGGATGGAGGATTACAGCAAGATGGAAATACCAAATCCCCCTACCTCCAAATGTATCACTT
ACTGGAAAAGAAAAGTGAAATCTGAATACATGCGACTTCGACAACTTAAACGGCTTCAGGCAAATATGGGTGCAAAGGCTTTGTATGTGG
CAAATTTTGCAAAGGTTCAAGAAAAAACCCAGATCCTCAATGAAGAATGGAAGAAGCTTCGTGTCCAACCTGTTCAGTCAATGAAGCCTG
TGAGTGGACACCCTTTTCTCAAAAAGTGTACCATAGAGAGCATTTTCCCGGGATTTGCAAGCCAACATATGTTAATGAGGTCACTGAACA
CAGTTGCATTGGTTCCCATCATGTATTCCTGGTCCCCTCTCCAACAGAACTTTATGGTAGAAGATGAGACGGTTTTGTGCAATATTCCCT
ACATGGGAGATGAAGTGAAAGAAGAAGATGAGACTTTTATTGAGGAGCTGATCAATAACTATGATGGGAAAGTCCATGGTGAAGAAGAGA
TGATCCCTGGATCCGTTCTGATTAGTGATGCTGTTTTTCTGGAGTTGGTCGATGCCCTGAATCAGTACTCAGATGAGGAGGAGGAAGGGC
ACAATGACACCTCAGATGGAAAGCAGGATGACAGCAAAGAAGATCTGCCAGTAACAAGAAAGAGAAAGCGACATGCTATTGAAGGCAACA
AAAAGAGTTCCAAGAAACAGTTCCCAAATGACATGATCTTCAGTGCAATTGCCTCAATGTTCCCTGAGAATGGTGTCCCAGATGACATGA
AGGAGAGGTATCGAGAACTAACAGAGATGTCAGACCCCAATGCACTTCCCCCTCAGTGCACACCCAACATCGATGGCCCCAATGCCAAGT
CTGTGCAGCGGGAGCAATCTCTGCACTCCTTCCACACACTTTTTTGCCGGCGCTGCTTTAAATACGACTGCTTCCTTCACCCTTTTCATG
CCACCCCTAATGTATATAAACGCAAGAATAAAGAAATCAAGATTGAACCAGAACCATGTGGCACAGACTGCTTCCTTTTGCTGGAAGGAG
CAAAGGAGTATGCCATGCTCCACAACCCCCGCTCCAAGTGCTCTGGTCGTCGCCGGAGAAGGCACCACATAGTCAGTGCTTCCTGCTCCA
ATGCCTCAGCCTCTGCTGTGGCTGAGACTAAAGAAGGAGACAGTGACAGGGACACAGGCAATGACTGGGCCTCCAGTTCTTCAGAGGCTA
ACTCTCGCTGTCAGACTCCCACAAAACAGAAGGCTAGTCCAGCCCCACCTCAACTCTGCGTAGTGGAAGCACCCTCGGAGCCTGTGGAAT
GGACTGGGGCTGAAGAATCTCTTTTTCGAGTCTTCCATGGCACCTACTTCAACAACTTCTGTTCAATAGCCAGGCTTCTGGGGACCAAGA
CGTGCAAGCAGGTCTTTCAGTTTGCAGTCAAAGAATCACTTATCCTGAAGCTGCCAACAGATGAGCTCATGAACCCCTCACAGAAGAAGA
AAAGAAAGCACAGATTGTGGGCTGCACACTGCAGGAAGATTCAGCTGAAGAAAGATAACTCTTCCACACAAGTGTACAACTACCAACCCT
GCGACCACCCAGACCGCCCCTGTGACAGCACCTGCCCCTGCATCATGACTCAGAATTTCTGTGAGAAGTTCTGCCAGTGCAACCCAGACT
GTCAGAATCGTTTCCCTGGCTGTCGCTGTAAGACCCAGTGCAATACCAAGCAATGTCCTTGCTATCTGGCAGTGCGAGAATGTGACCCTG
ACCTGTGTCTCACCTGTGGGGCCTCAGAGCACTGGGACTGCAAGGTGGTTTCCTGTAAAAACTGCAGCATCCAGCGTGGACTTAAGAAGC
ACCTGCTGCTGGCCCCCTCTGATGTGGCCGGATGGGGCACCTTCATAAAGGAGTCTGTGCAGAAGAACGAATTCATTTCTGAATACTGTG
GTGAGCTCATCTCTCAGGATGAGGCTGATCGACGCGGAAAGGTCTATGACAAATACATGTCCAGCTTCCTCTTCAACCTCAATAATGATT
TTGTAGTGGATGCTACTCGGAAAGGAAACAAAATTCGATTTGCAAATCATTCAGTGAATCCCAACTGTTATGCCAAAGTGGTCATGGTGA
ATGGAGACCATCGGATTGGGATCTTTGCCAAGAGGGCAATTCAAGCTGGCGAAGAGCTCTTCTTTGATTACAGGCCTTTAGCAAAAAACA
GATAGATGATCGAAAGGAATGGTTAACTAATTTCATGGAGGATAGAAGACAACGAAAGTTACTTGGGCTTCCTGAGGATTACTTGTATGG
ACAAACTACCACATATCTGACATATAATGACTTCATCAACAAGGAACTTATCTTGTTCTCAAATTCTGATAACGAGAGATCTATCCCTTC
TATGGTGGATGGTTTGAAACCAGGTCAGAGAAAGGTTTTGTTTACTTGCTTCAAACGGAATGACAAGCGAGAAGTAAAGGTTGCCCAATT
AGCTGGATCAGTGGCTGAAATGTCTTCTTATCATCATGGTGAGATGTCACTAATGATGACCATTATCAATTTGGCTCAGAATTTTGTGGG
TAGCAATAATCTAAACCTCTTGCAGCCCATTGGTCAGTTTGGTACCAGGCTACATGGTGGCAAGGATTCTGCTAGTCCACGATACATCTT
TACAATGCTCAGCTCTTTGGCTCGATTGTTATTTCCACCAAAAGATGATCACACGTTGAAGTTTTTATATGATGACAACCAGCGTGTTGA
GCCTGAATGGTACATTCCTATTATTCCCATGGTGCTGATAAATGGTGCTGAAGGAATCGGTACTGGGTGGTCCTGCAAAATCCCCAACTT
TGATGTGCGTGAAATTGTAAATAACATCAGGCGTTTGATGGATGGAGAAGAACCTTTGCCAATGCTTCCAAGTTACAAGAACTTCAAGGG
TACTATTGAAGAACTGGCTCCAAATCAATATGTGATTAGTGGTGAAGTAGCTATTCTTAATTCTACAACCATTGAAATCTCAGAGCTTCC
CGTCAGAACATGGACCCAGACATACAAAGAACAAGTTCTAGAACCCATGTTGAATGGCACCGAGAAGACACCTCCTCTCATAACAGACTA
TAGGGAATACCATACAGATACCACTGTGAAATTTGTTGTGAAGATGACTGAAGAAAAACTGGCAGAGGCAGAGAGAGTTGGACTACACAA
AGTCTTCAAACTCCAAACTAGTCTCACATGCAACTCTATGGTGCTTTTTGACCACGTAGGCTGTTTAAAGAAATATGACACGGTGTTGGA
TATTCTAAGAGACTTTTTTGAACTCAGACTTAAATATTATGGATTAAGAAAAGAATGGCTCCTAGGAATGCTTGGTGCTGAATCTGCTAA
ACTGAATAATCAGGCTCGCTTTATCTTAGAGAAAATAGATGGCAAAATAATCATTGAAAATAAGCCTAAGAAAGAATTAATTAAAGTTCT
GATTCAGAGGGGATATGATTCGGATCCTGTGAAGGCCTGGAAAGAAGCCCAGCAAAAGGTTCCAGATGAAGAAGAAAATGAAGAGAGTGA
CAACGAAAAGGAAACTGAAAAGAGTGACTCCGTAACAGATTCTGGACCAACCTTCAACTATCTTCTTGATATGCCCCTTTGGTATTTAAC
CAAGGAAAAGAAAGATGAACTCTGCAGGCTAAGAAATGAAAAAGAACAAGAGCTGGACACATTAAAAAGAAAGAGTCCATCAGATTTGTG
GAAAGAAGACTTGGCTACATTTATTGAAGAATTGGAGGCTGTTGAAGCCAAGGAAAAACAAGATGAACAAGTCGGACTTCCTGGGAAAGG
GGGGAAGGCCAAGGGGAAAAAAACACAAATGGCTGAAGTTTTGCCTTCTCCGCGTGGTCAAAGAGTCATTCCACGAATAACCATAGAAAT
GAAAGCAGAGGCAGAAAAGAAAAATAAAAAGAAAATTAAGAATGAAAATACTGAAGGAAGCCCTCAAGAAGATGGTGTGGAACTAGAAGG
CCTAAAACAAAGATTAGAAAAGAAACAGAAAAGAGAACCAGGTACAAAGACAAAGAAACAAACTACATTGGCATTTAAGCCAATCAAAAA
AGGAAAGAAGAGAAATCCCTGGTCTGATTCAGAATCAGATAGGAGCAGTGACGAAAGTAATTTTGATGTCCCTCCACGAGAAACAGAGCC
ACGGAGAGCAGCAACAAAAACAAAATTCACAATGGATTTGGATTCAGATGAAGATTTCTCAGATTTTGATGAAAAAACTGATGATGAAGA
TTTTGTCCCATCAGATGCTAGTCCACCTAAGACCAAAACTTCCCCAAAACTTAGTAACAAAGAACTGAAACCACAGAAAAGTGTCGTGTC
AGACCTTGAAGCTGATGATGTTAAGGGCAGTGTACCACTGTCTTCAAGCCCTCCTGCTACACATTTCCCAGATGAAACTGAAATTACAAA
CCCAGTTCCTAAAAAGAATGTGACAGTGAAGAAGACAGCAGCAAAAAGTCAGTCTTCCACCTCCACTACCGGTGCCAAAAAAAGGGCTGC
CCCAAAAGGAACTAAAAGGGATCCAGCTTTGAATTCTGGTGTCTCTCAAAAGCCTGATCCTGCCAAAACCAAGAATCGCCGCAAAAGGAA
GCCATCCACTTCTGATGATTCTGACTCTAATTTTGAGAAAATTGTTTCGAAAGCAGTCACAAGCAAGAAATCCAAGGGGGAGAGTGATGA
CTTCCATATGGACTTTGACTCAGCTGTGGCTCCTCGGGCAAAATCTGTACGGGCAAAGAAACCTATAAAGTACCTGGAAGAGTCAGATGA
AGATGATCTGTTTTAAAATGTGAGGCGATTATTTTAAGTAATTATCTTACCAAGCCCAAGACTGGTTTTAAAGTTACCTGAAGCTCTTAA
CTTCCTCCCCTCTGAATTTAGTTTGGGGAAGGTGTTTTTAGTACAAGACATCAAAGTGAAGTAAAGCCCAAGTGTTCTTTAGCTTTTTAT
AATACTGTCTAAATAGTGACCATCTCATGGGCATTGTTTTCTTCTCTGCTTTGTCTGTGTTTTGAGTCTGCTTTCTTTTGTCTTTAAAAC
CTGATTTTTAAGTTCTTCTGAACTGTAGAAATAGCTATCTGATCACTTCAGCGTAAAGCAGTGTGTTTATTAACCATCCACTAAGCTAAA
ACTAGAGCAGTTTGATTTAAAAGTGTCACTCTTCCTCCTTTTCTACTTTCAGTAGATATGAGATAGAGCATAATTATCTGTTTTATCTTA
GTTTTATACATAATTTACCATCAGATAGAACTTTATGGTTCTAGTACAGATACTCTACTACACTCAGCCTCTTATGTGCCAAGTTTTTCT
TTAAGCAATGAGAAATTGCTCATGTTCTTCATCTTCTCAAATCATCAGAGGCCGAAGAAAAACACTTTGGCTGTGTCTATAACTTGACAC
AGTCAATAGAATGAAGAAAATTAGAGTAGTTATGTGATTATTTCAGCTCTTGACCTGTCCCCTCTGGCTGCCTCTGAGTCTGAATCTCCC
AAAGAGAGAAACCAATTTCTAAGAGGACTGGATTGCAGAAGACTCGGGGACAACATTTGATCCAAGATCTTAAATGTTATATTGATAACC
ATGCTCAGCAATGAGCTATTAGATTCATTTTGGGAAATCTCCATAATTTCAATTTGTAAACTTTGTTAAGACCTGTCTACATTGTTATAT
GTGTGTGACTTGAGTAATGTTATCAACGTTTTTGTAAATATTTACTATGTTTTTCTATTAGCTAAATTCCAACAATTTTGTACTTTAATA

>In-frame_ENST00000592743_ENST00000423485_TCGA-B6-A0IG-01A_EZH1_chr17_40854895_-_TOP2A_chr17_38561135_length(amino acids)=863AA_start in transcript=2284_stop in transcript=4875
MEDRRQRKLLGLPEDYLYGQTTTYLTYNDFINKELILFSNSDNERSIPSMVDGLKPGQRKVLFTCFKRNDKREVKVAQLAGSVAEMSSYH
HGEMSLMMTIINLAQNFVGSNNLNLLQPIGQFGTRLHGGKDSASPRYIFTMLSSLARLLFPPKDDHTLKFLYDDNQRVEPEWYIPIIPMV
LINGAEGIGTGWSCKIPNFDVREIVNNIRRLMDGEEPLPMLPSYKNFKGTIEELAPNQYVISGEVAILNSTTIEISELPVRTWTQTYKEQ
VLEPMLNGTEKTPPLITDYREYHTDTTVKFVVKMTEEKLAEAERVGLHKVFKLQTSLTCNSMVLFDHVGCLKKYDTVLDILRDFFELRLK
YYGLRKEWLLGMLGAESAKLNNQARFILEKIDGKIIIENKPKKELIKVLIQRGYDSDPVKAWKEAQQKVPDEEENEESDNEKETEKSDSV
TDSGPTFNYLLDMPLWYLTKEKKDELCRLRNEKEQELDTLKRKSPSDLWKEDLATFIEELEAVEAKEKQDEQVGLPGKGGKAKGKKTQMA
EVLPSPRGQRVIPRITIEMKAEAEKKNKKKIKNENTEGSPQEDGVELEGLKQRLEKKQKREPGTKTKKQTTLAFKPIKKGKKRNPWSDSE
SDRSSDESNFDVPPRETEPRRAATKTKFTMDLDSDEDFSDFDEKTDDEDFVPSDASPPKTKTSPKLSNKELKPQKSVVSDLEADDVKGSV
PLSSSPPATHFPDETEITNPVPKKNVTVKKTAAKSQSSTSTTGAKKRAAPKGTKRDPALNSGVSQKPDPAKTKNRRKRKPSTSDDSDSNF

--------------------------------------------------------------
>In-frame_ENST00000590078_ENST00000423485_TCGA-B6-A0IG-01A_EZH1_chr17_40854895_-_TOP2A_chr17_38561135_length(transcript)=5830nt_BP=2184nt
AGCGGGACCCGCGGCTCGGGATGGAGGCTGGACACCTGTTCTGCTGTTGTGTCCTGCCATTCTCCTGAAGAACAGAGGCACACTGTAAAA
CCCAACACTTCCCCTTGCATTCTATAAGGCTTTGTATGTGGCAAATTTTGCAAAGGTTCAAGAAAAAACCCAGATCCTCAATGAAGAATG
GAAGAAGCTTCGTGTCCAACCTGTTCAGTCAATGAAGCCTGTGAGTGGACACCCTTTTCTCAAAAAGTGTACCATAGAGAGCATTTTCCC
GGGATTTGCAAGCCAACATATGTTAATGAGGTCACTGAACACAGTTGCATTGGTTCCCATCATGTATTCCTGGTCCCCTCTCCAACAGAA
CTTTATGGTAGAAGATGAGACGGTTTTGTGCAATATTCCCTACATGGGAGATGAAGTGAAAGAAGAAGATGAGACTTTTATTGAGGAGCT
GATCAATAACTATGATGGGAAAGTCCATGGTGAAGAAGAGATGATCCCTGGATCCGTTCTGATTAGTGATGCTGTTTTTCTGGAGTTGGT
CGATGCCCTGAATCAGTACTCAGATGAGGAGGAGGAAGGGCACAATGACACCTCAGATGGAAAGCAGGATGACAGCAAAGAAGATCTGCC
AGTAACAAGAAAGAGAAAGCGACATGCTATTGAAGGCAACAAAAAGAGTTCCAAGAAACAGTTCCCAAATGACATGATCTTCAGTGCAAT
TGCCTCAATGTTCCCTGAGAATGGTGTCCCAGATGACATGAAGGAGAGGTATCGAGAACTAACAGAGATGTCAGACCCCAATGCACTTCC
CCCTCAGTGCACACCCAACATCGATGGCCCCAATGCCAAGTCTGTGCAGCGGGAGCAATCTCTGCACTCCTTCCACACACTTTTTTGCCG
GCGCTGCTTTAAATACGACTGCTTCCTTCACCCTTTTCATGCCACCCCTAATGTATATAAACGCAAGAATAAAGAAATCAAGATTGAACC
AGAACCATGTGGCACAGACTGCTTCCTTTTGCTGGAAGGAGCAAAGGAGTATGCCATGCTCCACAACCCCCGCTCCAAGTGCTCTGGTCG
TCGCCGGAGAAGGCACCACATAGTCAGTGCTTCCTGCTCCAATGCCTCAGCCTCTGCTGTGGCTGAGACTAAAGAAGGAGACAGTGACAG
GGACACAGGCAATGACTGGGCCTCCAGTTCTTCAGAGGCTAACTCTCGCTGTCAGACTCCCACAAAACAGAAGGCTAGTCCAGCCCCACC
TCAACTCTGCGTAGTGGAAGCACCCTCGGAGCCTGTGGAATGGACTGGGGCTGAAGAATCTCTTTTTCGAGTCTTCCATGGCACCTACTT
CAACAACTTCTGTTCAATAGCCAGGCTTCTGGGGACCAAGACGTGCAAGCAGGTCTTTCAGTTTGCAGTCAAAGAATCACTTATCCTGAA
GCTGCCAACAGATGAGCTCATGAACCCCTCACAGAAGAAGAAAAGAAAGCACAGATTGTGGGCTGCACACTGCAGGAAGATTCAGCTGAA
GAAAGATAACTCTTCCACACAAGTGTACAACTACCAACCCTGCGACCACCCAGACCGCCCCTGTGACAGCACCTGCCCCTGCATCATGAC
TCAGAATTTCTGTGAGAAGTTCTGCCAGTGCAACCCAGACTGTCAGAATCGTTTCCCTGGCTGTCGCTGTAAGACCCAGTGCAATACCAA
GCAATGTCCTTGCTATCTGGCAGTGCGAGAATGTGACCCTGACCTGTGTCTCACCTGTGGGGCCTCAGAGCACTGGGACTGCAAGGTGGT
TTCCTGTAAAAACTGCAGCATCCAGCGTGGACTTAAGAAGCACCTGCTGCTGGCCCCCTCTGATGTGGCCGGATGGGGCACCTTCATAAA
GGAGTCTGTGCAGAAGAACGAATTCATTTCTGAATACTGTGGTGAGCTCATCTCTCAGGATGAGGCTGATCGACGCGGAAAGGTCTATGA
CAAATACATGTCCAGCTTCCTCTTCAACCTCAATAATGATTTTGTAGTGGATGCTACTCGGAAAGGAAACAAAATTCGATTTGCAAATCA
TTCAGTGAATCCCAACTGTTATGCCAAAGTGGTCATGGTGAATGGAGACCATCGGATTGGGATCTTTGCCAAGAGGGCAATTCAAGCTGG
CGAAGAGCTCTTCTTTGATTACAGGCCTTTAGCAAAAAACAGATAGATGATCGAAAGGAATGGTTAACTAATTTCATGGAGGATAGAAGA
CAACGAAAGTTACTTGGGCTTCCTGAGGATTACTTGTATGGACAAACTACCACATATCTGACATATAATGACTTCATCAACAAGGAACTT
ATCTTGTTCTCAAATTCTGATAACGAGAGATCTATCCCTTCTATGGTGGATGGTTTGAAACCAGGTCAGAGAAAGGTTTTGTTTACTTGC
TTCAAACGGAATGACAAGCGAGAAGTAAAGGTTGCCCAATTAGCTGGATCAGTGGCTGAAATGTCTTCTTATCATCATGGTGAGATGTCA
CTAATGATGACCATTATCAATTTGGCTCAGAATTTTGTGGGTAGCAATAATCTAAACCTCTTGCAGCCCATTGGTCAGTTTGGTACCAGG
CTACATGGTGGCAAGGATTCTGCTAGTCCACGATACATCTTTACAATGCTCAGCTCTTTGGCTCGATTGTTATTTCCACCAAAAGATGAT
CACACGTTGAAGTTTTTATATGATGACAACCAGCGTGTTGAGCCTGAATGGTACATTCCTATTATTCCCATGGTGCTGATAAATGGTGCT
GAAGGAATCGGTACTGGGTGGTCCTGCAAAATCCCCAACTTTGATGTGCGTGAAATTGTAAATAACATCAGGCGTTTGATGGATGGAGAA
GAACCTTTGCCAATGCTTCCAAGTTACAAGAACTTCAAGGGTACTATTGAAGAACTGGCTCCAAATCAATATGTGATTAGTGGTGAAGTA
GCTATTCTTAATTCTACAACCATTGAAATCTCAGAGCTTCCCGTCAGAACATGGACCCAGACATACAAAGAACAAGTTCTAGAACCCATG
TTGAATGGCACCGAGAAGACACCTCCTCTCATAACAGACTATAGGGAATACCATACAGATACCACTGTGAAATTTGTTGTGAAGATGACT
GAAGAAAAACTGGCAGAGGCAGAGAGAGTTGGACTACACAAAGTCTTCAAACTCCAAACTAGTCTCACATGCAACTCTATGGTGCTTTTT
GACCACGTAGGCTGTTTAAAGAAATATGACACGGTGTTGGATATTCTAAGAGACTTTTTTGAACTCAGACTTAAATATTATGGATTAAGA
AAAGAATGGCTCCTAGGAATGCTTGGTGCTGAATCTGCTAAACTGAATAATCAGGCTCGCTTTATCTTAGAGAAAATAGATGGCAAAATA
ATCATTGAAAATAAGCCTAAGAAAGAATTAATTAAAGTTCTGATTCAGAGGGGATATGATTCGGATCCTGTGAAGGCCTGGAAAGAAGCC
CAGCAAAAGGTTCCAGATGAAGAAGAAAATGAAGAGAGTGACAACGAAAAGGAAACTGAAAAGAGTGACTCCGTAACAGATTCTGGACCA
ACCTTCAACTATCTTCTTGATATGCCCCTTTGGTATTTAACCAAGGAAAAGAAAGATGAACTCTGCAGGCTAAGAAATGAAAAAGAACAA
GAGCTGGACACATTAAAAAGAAAGAGTCCATCAGATTTGTGGAAAGAAGACTTGGCTACATTTATTGAAGAATTGGAGGCTGTTGAAGCC
AAGGAAAAACAAGATGAACAAGTCGGACTTCCTGGGAAAGGGGGGAAGGCCAAGGGGAAAAAAACACAAATGGCTGAAGTTTTGCCTTCT
CCGCGTGGTCAAAGAGTCATTCCACGAATAACCATAGAAATGAAAGCAGAGGCAGAAAAGAAAAATAAAAAGAAAATTAAGAATGAAAAT
ACTGAAGGAAGCCCTCAAGAAGATGGTGTGGAACTAGAAGGCCTAAAACAAAGATTAGAAAAGAAACAGAAAAGAGAACCAGGTACAAAG
ACAAAGAAACAAACTACATTGGCATTTAAGCCAATCAAAAAAGGAAAGAAGAGAAATCCCTGGTCTGATTCAGAATCAGATAGGAGCAGT
GACGAAAGTAATTTTGATGTCCCTCCACGAGAAACAGAGCCACGGAGAGCAGCAACAAAAACAAAATTCACAATGGATTTGGATTCAGAT
GAAGATTTCTCAGATTTTGATGAAAAAACTGATGATGAAGATTTTGTCCCATCAGATGCTAGTCCACCTAAGACCAAAACTTCCCCAAAA
CTTAGTAACAAAGAACTGAAACCACAGAAAAGTGTCGTGTCAGACCTTGAAGCTGATGATGTTAAGGGCAGTGTACCACTGTCTTCAAGC
CCTCCTGCTACACATTTCCCAGATGAAACTGAAATTACAAACCCAGTTCCTAAAAAGAATGTGACAGTGAAGAAGACAGCAGCAAAAAGT
CAGTCTTCCACCTCCACTACCGGTGCCAAAAAAAGGGCTGCCCCAAAAGGAACTAAAAGGGATCCAGCTTTGAATTCTGGTGTCTCTCAA
AAGCCTGATCCTGCCAAAACCAAGAATCGCCGCAAAAGGAAGCCATCCACTTCTGATGATTCTGACTCTAATTTTGAGAAAATTGTTTCG
AAAGCAGTCACAAGCAAGAAATCCAAGGGGGAGAGTGATGACTTCCATATGGACTTTGACTCAGCTGTGGCTCCTCGGGCAAAATCTGTA
CGGGCAAAGAAACCTATAAAGTACCTGGAAGAGTCAGATGAAGATGATCTGTTTTAAAATGTGAGGCGATTATTTTAAGTAATTATCTTA
CCAAGCCCAAGACTGGTTTTAAAGTTACCTGAAGCTCTTAACTTCCTCCCCTCTGAATTTAGTTTGGGGAAGGTGTTTTTAGTACAAGAC
ATCAAAGTGAAGTAAAGCCCAAGTGTTCTTTAGCTTTTTATAATACTGTCTAAATAGTGACCATCTCATGGGCATTGTTTTCTTCTCTGC
TTTGTCTGTGTTTTGAGTCTGCTTTCTTTTGTCTTTAAAACCTGATTTTTAAGTTCTTCTGAACTGTAGAAATAGCTATCTGATCACTTC
AGCGTAAAGCAGTGTGTTTATTAACCATCCACTAAGCTAAAACTAGAGCAGTTTGATTTAAAAGTGTCACTCTTCCTCCTTTTCTACTTT
CAGTAGATATGAGATAGAGCATAATTATCTGTTTTATCTTAGTTTTATACATAATTTACCATCAGATAGAACTTTATGGTTCTAGTACAG
ATACTCTACTACACTCAGCCTCTTATGTGCCAAGTTTTTCTTTAAGCAATGAGAAATTGCTCATGTTCTTCATCTTCTCAAATCATCAGA
GGCCGAAGAAAAACACTTTGGCTGTGTCTATAACTTGACACAGTCAATAGAATGAAGAAAATTAGAGTAGTTATGTGATTATTTCAGCTC
TTGACCTGTCCCCTCTGGCTGCCTCTGAGTCTGAATCTCCCAAAGAGAGAAACCAATTTCTAAGAGGACTGGATTGCAGAAGACTCGGGG
ACAACATTTGATCCAAGATCTTAAATGTTATATTGATAACCATGCTCAGCAATGAGCTATTAGATTCATTTTGGGAAATCTCCATAATTT
CAATTTGTAAACTTTGTTAAGACCTGTCTACATTGTTATATGTGTGTGACTTGAGTAATGTTATCAACGTTTTTGTAAATATTTACTATG

>In-frame_ENST00000590078_ENST00000423485_TCGA-B6-A0IG-01A_EZH1_chr17_40854895_-_TOP2A_chr17_38561135_length(amino acids)=863AA_start in transcript=2235_stop in transcript=4826
MEDRRQRKLLGLPEDYLYGQTTTYLTYNDFINKELILFSNSDNERSIPSMVDGLKPGQRKVLFTCFKRNDKREVKVAQLAGSVAEMSSYH
HGEMSLMMTIINLAQNFVGSNNLNLLQPIGQFGTRLHGGKDSASPRYIFTMLSSLARLLFPPKDDHTLKFLYDDNQRVEPEWYIPIIPMV
LINGAEGIGTGWSCKIPNFDVREIVNNIRRLMDGEEPLPMLPSYKNFKGTIEELAPNQYVISGEVAILNSTTIEISELPVRTWTQTYKEQ
VLEPMLNGTEKTPPLITDYREYHTDTTVKFVVKMTEEKLAEAERVGLHKVFKLQTSLTCNSMVLFDHVGCLKKYDTVLDILRDFFELRLK
YYGLRKEWLLGMLGAESAKLNNQARFILEKIDGKIIIENKPKKELIKVLIQRGYDSDPVKAWKEAQQKVPDEEENEESDNEKETEKSDSV
TDSGPTFNYLLDMPLWYLTKEKKDELCRLRNEKEQELDTLKRKSPSDLWKEDLATFIEELEAVEAKEKQDEQVGLPGKGGKAKGKKTQMA
EVLPSPRGQRVIPRITIEMKAEAEKKNKKKIKNENTEGSPQEDGVELEGLKQRLEKKQKREPGTKTKKQTTLAFKPIKKGKKRNPWSDSE
SDRSSDESNFDVPPRETEPRRAATKTKFTMDLDSDEDFSDFDEKTDDEDFVPSDASPPKTKTSPKLSNKELKPQKSVVSDLEADDVKGSV
PLSSSPPATHFPDETEITNPVPKKNVTVKKTAAKSQSSTSTTGAKKRAAPKGTKRDPALNSGVSQKPDPAKTKNRRKRKPSTSDDSDSNF

--------------------------------------------------------------
>In-frame_ENST00000592743_ENST00000423485_TCGA-B6-A0IG_EZH1_chr17_40854894_-_TOP2A_chr17_38561135_length(transcript)=5879nt_BP=2233nt
ATGCGTCCTAGCAGCGGGACCCGCGGCTCGGGATGGAGGATTACAGCAAGATGGAAATACCAAATCCCCCTACCTCCAAATGTATCACTT
ACTGGAAAAGAAAAGTGAAATCTGAATACATGCGACTTCGACAACTTAAACGGCTTCAGGCAAATATGGGTGCAAAGGCTTTGTATGTGG
CAAATTTTGCAAAGGTTCAAGAAAAAACCCAGATCCTCAATGAAGAATGGAAGAAGCTTCGTGTCCAACCTGTTCAGTCAATGAAGCCTG
TGAGTGGACACCCTTTTCTCAAAAAGTGTACCATAGAGAGCATTTTCCCGGGATTTGCAAGCCAACATATGTTAATGAGGTCACTGAACA
CAGTTGCATTGGTTCCCATCATGTATTCCTGGTCCCCTCTCCAACAGAACTTTATGGTAGAAGATGAGACGGTTTTGTGCAATATTCCCT
ACATGGGAGATGAAGTGAAAGAAGAAGATGAGACTTTTATTGAGGAGCTGATCAATAACTATGATGGGAAAGTCCATGGTGAAGAAGAGA
TGATCCCTGGATCCGTTCTGATTAGTGATGCTGTTTTTCTGGAGTTGGTCGATGCCCTGAATCAGTACTCAGATGAGGAGGAGGAAGGGC
ACAATGACACCTCAGATGGAAAGCAGGATGACAGCAAAGAAGATCTGCCAGTAACAAGAAAGAGAAAGCGACATGCTATTGAAGGCAACA
AAAAGAGTTCCAAGAAACAGTTCCCAAATGACATGATCTTCAGTGCAATTGCCTCAATGTTCCCTGAGAATGGTGTCCCAGATGACATGA
AGGAGAGGTATCGAGAACTAACAGAGATGTCAGACCCCAATGCACTTCCCCCTCAGTGCACACCCAACATCGATGGCCCCAATGCCAAGT
CTGTGCAGCGGGAGCAATCTCTGCACTCCTTCCACACACTTTTTTGCCGGCGCTGCTTTAAATACGACTGCTTCCTTCACCCTTTTCATG
CCACCCCTAATGTATATAAACGCAAGAATAAAGAAATCAAGATTGAACCAGAACCATGTGGCACAGACTGCTTCCTTTTGCTGGAAGGAG
CAAAGGAGTATGCCATGCTCCACAACCCCCGCTCCAAGTGCTCTGGTCGTCGCCGGAGAAGGCACCACATAGTCAGTGCTTCCTGCTCCA
ATGCCTCAGCCTCTGCTGTGGCTGAGACTAAAGAAGGAGACAGTGACAGGGACACAGGCAATGACTGGGCCTCCAGTTCTTCAGAGGCTA
ACTCTCGCTGTCAGACTCCCACAAAACAGAAGGCTAGTCCAGCCCCACCTCAACTCTGCGTAGTGGAAGCACCCTCGGAGCCTGTGGAAT
GGACTGGGGCTGAAGAATCTCTTTTTCGAGTCTTCCATGGCACCTACTTCAACAACTTCTGTTCAATAGCCAGGCTTCTGGGGACCAAGA
CGTGCAAGCAGGTCTTTCAGTTTGCAGTCAAAGAATCACTTATCCTGAAGCTGCCAACAGATGAGCTCATGAACCCCTCACAGAAGAAGA
AAAGAAAGCACAGATTGTGGGCTGCACACTGCAGGAAGATTCAGCTGAAGAAAGATAACTCTTCCACACAAGTGTACAACTACCAACCCT
GCGACCACCCAGACCGCCCCTGTGACAGCACCTGCCCCTGCATCATGACTCAGAATTTCTGTGAGAAGTTCTGCCAGTGCAACCCAGACT
GTCAGAATCGTTTCCCTGGCTGTCGCTGTAAGACCCAGTGCAATACCAAGCAATGTCCTTGCTATCTGGCAGTGCGAGAATGTGACCCTG
ACCTGTGTCTCACCTGTGGGGCCTCAGAGCACTGGGACTGCAAGGTGGTTTCCTGTAAAAACTGCAGCATCCAGCGTGGACTTAAGAAGC
ACCTGCTGCTGGCCCCCTCTGATGTGGCCGGATGGGGCACCTTCATAAAGGAGTCTGTGCAGAAGAACGAATTCATTTCTGAATACTGTG
GTGAGCTCATCTCTCAGGATGAGGCTGATCGACGCGGAAAGGTCTATGACAAATACATGTCCAGCTTCCTCTTCAACCTCAATAATGATT
TTGTAGTGGATGCTACTCGGAAAGGAAACAAAATTCGATTTGCAAATCATTCAGTGAATCCCAACTGTTATGCCAAAGTGGTCATGGTGA
ATGGAGACCATCGGATTGGGATCTTTGCCAAGAGGGCAATTCAAGCTGGCGAAGAGCTCTTCTTTGATTACAGGCCTTTAGCAAAAAACA
GATAGATGATCGAAAGGAATGGTTAACTAATTTCATGGAGGATAGAAGACAACGAAAGTTACTTGGGCTTCCTGAGGATTACTTGTATGG
ACAAACTACCACATATCTGACATATAATGACTTCATCAACAAGGAACTTATCTTGTTCTCAAATTCTGATAACGAGAGATCTATCCCTTC
TATGGTGGATGGTTTGAAACCAGGTCAGAGAAAGGTTTTGTTTACTTGCTTCAAACGGAATGACAAGCGAGAAGTAAAGGTTGCCCAATT
AGCTGGATCAGTGGCTGAAATGTCTTCTTATCATCATGGTGAGATGTCACTAATGATGACCATTATCAATTTGGCTCAGAATTTTGTGGG
TAGCAATAATCTAAACCTCTTGCAGCCCATTGGTCAGTTTGGTACCAGGCTACATGGTGGCAAGGATTCTGCTAGTCCACGATACATCTT
TACAATGCTCAGCTCTTTGGCTCGATTGTTATTTCCACCAAAAGATGATCACACGTTGAAGTTTTTATATGATGACAACCAGCGTGTTGA
GCCTGAATGGTACATTCCTATTATTCCCATGGTGCTGATAAATGGTGCTGAAGGAATCGGTACTGGGTGGTCCTGCAAAATCCCCAACTT
TGATGTGCGTGAAATTGTAAATAACATCAGGCGTTTGATGGATGGAGAAGAACCTTTGCCAATGCTTCCAAGTTACAAGAACTTCAAGGG
TACTATTGAAGAACTGGCTCCAAATCAATATGTGATTAGTGGTGAAGTAGCTATTCTTAATTCTACAACCATTGAAATCTCAGAGCTTCC
CGTCAGAACATGGACCCAGACATACAAAGAACAAGTTCTAGAACCCATGTTGAATGGCACCGAGAAGACACCTCCTCTCATAACAGACTA
TAGGGAATACCATACAGATACCACTGTGAAATTTGTTGTGAAGATGACTGAAGAAAAACTGGCAGAGGCAGAGAGAGTTGGACTACACAA
AGTCTTCAAACTCCAAACTAGTCTCACATGCAACTCTATGGTGCTTTTTGACCACGTAGGCTGTTTAAAGAAATATGACACGGTGTTGGA
TATTCTAAGAGACTTTTTTGAACTCAGACTTAAATATTATGGATTAAGAAAAGAATGGCTCCTAGGAATGCTTGGTGCTGAATCTGCTAA
ACTGAATAATCAGGCTCGCTTTATCTTAGAGAAAATAGATGGCAAAATAATCATTGAAAATAAGCCTAAGAAAGAATTAATTAAAGTTCT
GATTCAGAGGGGATATGATTCGGATCCTGTGAAGGCCTGGAAAGAAGCCCAGCAAAAGGTTCCAGATGAAGAAGAAAATGAAGAGAGTGA
CAACGAAAAGGAAACTGAAAAGAGTGACTCCGTAACAGATTCTGGACCAACCTTCAACTATCTTCTTGATATGCCCCTTTGGTATTTAAC
CAAGGAAAAGAAAGATGAACTCTGCAGGCTAAGAAATGAAAAAGAACAAGAGCTGGACACATTAAAAAGAAAGAGTCCATCAGATTTGTG
GAAAGAAGACTTGGCTACATTTATTGAAGAATTGGAGGCTGTTGAAGCCAAGGAAAAACAAGATGAACAAGTCGGACTTCCTGGGAAAGG
GGGGAAGGCCAAGGGGAAAAAAACACAAATGGCTGAAGTTTTGCCTTCTCCGCGTGGTCAAAGAGTCATTCCACGAATAACCATAGAAAT
GAAAGCAGAGGCAGAAAAGAAAAATAAAAAGAAAATTAAGAATGAAAATACTGAAGGAAGCCCTCAAGAAGATGGTGTGGAACTAGAAGG
CCTAAAACAAAGATTAGAAAAGAAACAGAAAAGAGAACCAGGTACAAAGACAAAGAAACAAACTACATTGGCATTTAAGCCAATCAAAAA
AGGAAAGAAGAGAAATCCCTGGTCTGATTCAGAATCAGATAGGAGCAGTGACGAAAGTAATTTTGATGTCCCTCCACGAGAAACAGAGCC
ACGGAGAGCAGCAACAAAAACAAAATTCACAATGGATTTGGATTCAGATGAAGATTTCTCAGATTTTGATGAAAAAACTGATGATGAAGA
TTTTGTCCCATCAGATGCTAGTCCACCTAAGACCAAAACTTCCCCAAAACTTAGTAACAAAGAACTGAAACCACAGAAAAGTGTCGTGTC
AGACCTTGAAGCTGATGATGTTAAGGGCAGTGTACCACTGTCTTCAAGCCCTCCTGCTACACATTTCCCAGATGAAACTGAAATTACAAA
CCCAGTTCCTAAAAAGAATGTGACAGTGAAGAAGACAGCAGCAAAAAGTCAGTCTTCCACCTCCACTACCGGTGCCAAAAAAAGGGCTGC
CCCAAAAGGAACTAAAAGGGATCCAGCTTTGAATTCTGGTGTCTCTCAAAAGCCTGATCCTGCCAAAACCAAGAATCGCCGCAAAAGGAA
GCCATCCACTTCTGATGATTCTGACTCTAATTTTGAGAAAATTGTTTCGAAAGCAGTCACAAGCAAGAAATCCAAGGGGGAGAGTGATGA
CTTCCATATGGACTTTGACTCAGCTGTGGCTCCTCGGGCAAAATCTGTACGGGCAAAGAAACCTATAAAGTACCTGGAAGAGTCAGATGA
AGATGATCTGTTTTAAAATGTGAGGCGATTATTTTAAGTAATTATCTTACCAAGCCCAAGACTGGTTTTAAAGTTACCTGAAGCTCTTAA
CTTCCTCCCCTCTGAATTTAGTTTGGGGAAGGTGTTTTTAGTACAAGACATCAAAGTGAAGTAAAGCCCAAGTGTTCTTTAGCTTTTTAT
AATACTGTCTAAATAGTGACCATCTCATGGGCATTGTTTTCTTCTCTGCTTTGTCTGTGTTTTGAGTCTGCTTTCTTTTGTCTTTAAAAC
CTGATTTTTAAGTTCTTCTGAACTGTAGAAATAGCTATCTGATCACTTCAGCGTAAAGCAGTGTGTTTATTAACCATCCACTAAGCTAAA
ACTAGAGCAGTTTGATTTAAAAGTGTCACTCTTCCTCCTTTTCTACTTTCAGTAGATATGAGATAGAGCATAATTATCTGTTTTATCTTA
GTTTTATACATAATTTACCATCAGATAGAACTTTATGGTTCTAGTACAGATACTCTACTACACTCAGCCTCTTATGTGCCAAGTTTTTCT
TTAAGCAATGAGAAATTGCTCATGTTCTTCATCTTCTCAAATCATCAGAGGCCGAAGAAAAACACTTTGGCTGTGTCTATAACTTGACAC
AGTCAATAGAATGAAGAAAATTAGAGTAGTTATGTGATTATTTCAGCTCTTGACCTGTCCCCTCTGGCTGCCTCTGAGTCTGAATCTCCC
AAAGAGAGAAACCAATTTCTAAGAGGACTGGATTGCAGAAGACTCGGGGACAACATTTGATCCAAGATCTTAAATGTTATATTGATAACC
ATGCTCAGCAATGAGCTATTAGATTCATTTTGGGAAATCTCCATAATTTCAATTTGTAAACTTTGTTAAGACCTGTCTACATTGTTATAT
GTGTGTGACTTGAGTAATGTTATCAACGTTTTTGTAAATATTTACTATGTTTTTCTATTAGCTAAATTCCAACAATTTTGTACTTTAATA

>In-frame_ENST00000592743_ENST00000423485_TCGA-B6-A0IG_EZH1_chr17_40854894_-_TOP2A_chr17_38561135_length(amino acids)=863AA_start in transcript=2284_stop in transcript=4875
MEDRRQRKLLGLPEDYLYGQTTTYLTYNDFINKELILFSNSDNERSIPSMVDGLKPGQRKVLFTCFKRNDKREVKVAQLAGSVAEMSSYH
HGEMSLMMTIINLAQNFVGSNNLNLLQPIGQFGTRLHGGKDSASPRYIFTMLSSLARLLFPPKDDHTLKFLYDDNQRVEPEWYIPIIPMV
LINGAEGIGTGWSCKIPNFDVREIVNNIRRLMDGEEPLPMLPSYKNFKGTIEELAPNQYVISGEVAILNSTTIEISELPVRTWTQTYKEQ
VLEPMLNGTEKTPPLITDYREYHTDTTVKFVVKMTEEKLAEAERVGLHKVFKLQTSLTCNSMVLFDHVGCLKKYDTVLDILRDFFELRLK
YYGLRKEWLLGMLGAESAKLNNQARFILEKIDGKIIIENKPKKELIKVLIQRGYDSDPVKAWKEAQQKVPDEEENEESDNEKETEKSDSV
TDSGPTFNYLLDMPLWYLTKEKKDELCRLRNEKEQELDTLKRKSPSDLWKEDLATFIEELEAVEAKEKQDEQVGLPGKGGKAKGKKTQMA
EVLPSPRGQRVIPRITIEMKAEAEKKNKKKIKNENTEGSPQEDGVELEGLKQRLEKKQKREPGTKTKKQTTLAFKPIKKGKKRNPWSDSE
SDRSSDESNFDVPPRETEPRRAATKTKFTMDLDSDEDFSDFDEKTDDEDFVPSDASPPKTKTSPKLSNKELKPQKSVVSDLEADDVKGSV
PLSSSPPATHFPDETEITNPVPKKNVTVKKTAAKSQSSTSTTGAKKRAAPKGTKRDPALNSGVSQKPDPAKTKNRRKRKPSTSDDSDSNF

--------------------------------------------------------------
>In-frame_ENST00000590078_ENST00000423485_TCGA-B6-A0IG_EZH1_chr17_40854894_-_TOP2A_chr17_38561135_length(transcript)=5830nt_BP=2184nt
AGCGGGACCCGCGGCTCGGGATGGAGGCTGGACACCTGTTCTGCTGTTGTGTCCTGCCATTCTCCTGAAGAACAGAGGCACACTGTAAAA
CCCAACACTTCCCCTTGCATTCTATAAGGCTTTGTATGTGGCAAATTTTGCAAAGGTTCAAGAAAAAACCCAGATCCTCAATGAAGAATG
GAAGAAGCTTCGTGTCCAACCTGTTCAGTCAATGAAGCCTGTGAGTGGACACCCTTTTCTCAAAAAGTGTACCATAGAGAGCATTTTCCC
GGGATTTGCAAGCCAACATATGTTAATGAGGTCACTGAACACAGTTGCATTGGTTCCCATCATGTATTCCTGGTCCCCTCTCCAACAGAA
CTTTATGGTAGAAGATGAGACGGTTTTGTGCAATATTCCCTACATGGGAGATGAAGTGAAAGAAGAAGATGAGACTTTTATTGAGGAGCT
GATCAATAACTATGATGGGAAAGTCCATGGTGAAGAAGAGATGATCCCTGGATCCGTTCTGATTAGTGATGCTGTTTTTCTGGAGTTGGT
CGATGCCCTGAATCAGTACTCAGATGAGGAGGAGGAAGGGCACAATGACACCTCAGATGGAAAGCAGGATGACAGCAAAGAAGATCTGCC
AGTAACAAGAAAGAGAAAGCGACATGCTATTGAAGGCAACAAAAAGAGTTCCAAGAAACAGTTCCCAAATGACATGATCTTCAGTGCAAT
TGCCTCAATGTTCCCTGAGAATGGTGTCCCAGATGACATGAAGGAGAGGTATCGAGAACTAACAGAGATGTCAGACCCCAATGCACTTCC
CCCTCAGTGCACACCCAACATCGATGGCCCCAATGCCAAGTCTGTGCAGCGGGAGCAATCTCTGCACTCCTTCCACACACTTTTTTGCCG
GCGCTGCTTTAAATACGACTGCTTCCTTCACCCTTTTCATGCCACCCCTAATGTATATAAACGCAAGAATAAAGAAATCAAGATTGAACC
AGAACCATGTGGCACAGACTGCTTCCTTTTGCTGGAAGGAGCAAAGGAGTATGCCATGCTCCACAACCCCCGCTCCAAGTGCTCTGGTCG
TCGCCGGAGAAGGCACCACATAGTCAGTGCTTCCTGCTCCAATGCCTCAGCCTCTGCTGTGGCTGAGACTAAAGAAGGAGACAGTGACAG
GGACACAGGCAATGACTGGGCCTCCAGTTCTTCAGAGGCTAACTCTCGCTGTCAGACTCCCACAAAACAGAAGGCTAGTCCAGCCCCACC
TCAACTCTGCGTAGTGGAAGCACCCTCGGAGCCTGTGGAATGGACTGGGGCTGAAGAATCTCTTTTTCGAGTCTTCCATGGCACCTACTT
CAACAACTTCTGTTCAATAGCCAGGCTTCTGGGGACCAAGACGTGCAAGCAGGTCTTTCAGTTTGCAGTCAAAGAATCACTTATCCTGAA
GCTGCCAACAGATGAGCTCATGAACCCCTCACAGAAGAAGAAAAGAAAGCACAGATTGTGGGCTGCACACTGCAGGAAGATTCAGCTGAA
GAAAGATAACTCTTCCACACAAGTGTACAACTACCAACCCTGCGACCACCCAGACCGCCCCTGTGACAGCACCTGCCCCTGCATCATGAC
TCAGAATTTCTGTGAGAAGTTCTGCCAGTGCAACCCAGACTGTCAGAATCGTTTCCCTGGCTGTCGCTGTAAGACCCAGTGCAATACCAA
GCAATGTCCTTGCTATCTGGCAGTGCGAGAATGTGACCCTGACCTGTGTCTCACCTGTGGGGCCTCAGAGCACTGGGACTGCAAGGTGGT
TTCCTGTAAAAACTGCAGCATCCAGCGTGGACTTAAGAAGCACCTGCTGCTGGCCCCCTCTGATGTGGCCGGATGGGGCACCTTCATAAA
GGAGTCTGTGCAGAAGAACGAATTCATTTCTGAATACTGTGGTGAGCTCATCTCTCAGGATGAGGCTGATCGACGCGGAAAGGTCTATGA
CAAATACATGTCCAGCTTCCTCTTCAACCTCAATAATGATTTTGTAGTGGATGCTACTCGGAAAGGAAACAAAATTCGATTTGCAAATCA
TTCAGTGAATCCCAACTGTTATGCCAAAGTGGTCATGGTGAATGGAGACCATCGGATTGGGATCTTTGCCAAGAGGGCAATTCAAGCTGG
CGAAGAGCTCTTCTTTGATTACAGGCCTTTAGCAAAAAACAGATAGATGATCGAAAGGAATGGTTAACTAATTTCATGGAGGATAGAAGA
CAACGAAAGTTACTTGGGCTTCCTGAGGATTACTTGTATGGACAAACTACCACATATCTGACATATAATGACTTCATCAACAAGGAACTT
ATCTTGTTCTCAAATTCTGATAACGAGAGATCTATCCCTTCTATGGTGGATGGTTTGAAACCAGGTCAGAGAAAGGTTTTGTTTACTTGC
TTCAAACGGAATGACAAGCGAGAAGTAAAGGTTGCCCAATTAGCTGGATCAGTGGCTGAAATGTCTTCTTATCATCATGGTGAGATGTCA
CTAATGATGACCATTATCAATTTGGCTCAGAATTTTGTGGGTAGCAATAATCTAAACCTCTTGCAGCCCATTGGTCAGTTTGGTACCAGG
CTACATGGTGGCAAGGATTCTGCTAGTCCACGATACATCTTTACAATGCTCAGCTCTTTGGCTCGATTGTTATTTCCACCAAAAGATGAT
CACACGTTGAAGTTTTTATATGATGACAACCAGCGTGTTGAGCCTGAATGGTACATTCCTATTATTCCCATGGTGCTGATAAATGGTGCT
GAAGGAATCGGTACTGGGTGGTCCTGCAAAATCCCCAACTTTGATGTGCGTGAAATTGTAAATAACATCAGGCGTTTGATGGATGGAGAA
GAACCTTTGCCAATGCTTCCAAGTTACAAGAACTTCAAGGGTACTATTGAAGAACTGGCTCCAAATCAATATGTGATTAGTGGTGAAGTA
GCTATTCTTAATTCTACAACCATTGAAATCTCAGAGCTTCCCGTCAGAACATGGACCCAGACATACAAAGAACAAGTTCTAGAACCCATG
TTGAATGGCACCGAGAAGACACCTCCTCTCATAACAGACTATAGGGAATACCATACAGATACCACTGTGAAATTTGTTGTGAAGATGACT
GAAGAAAAACTGGCAGAGGCAGAGAGAGTTGGACTACACAAAGTCTTCAAACTCCAAACTAGTCTCACATGCAACTCTATGGTGCTTTTT
GACCACGTAGGCTGTTTAAAGAAATATGACACGGTGTTGGATATTCTAAGAGACTTTTTTGAACTCAGACTTAAATATTATGGATTAAGA
AAAGAATGGCTCCTAGGAATGCTTGGTGCTGAATCTGCTAAACTGAATAATCAGGCTCGCTTTATCTTAGAGAAAATAGATGGCAAAATA
ATCATTGAAAATAAGCCTAAGAAAGAATTAATTAAAGTTCTGATTCAGAGGGGATATGATTCGGATCCTGTGAAGGCCTGGAAAGAAGCC
CAGCAAAAGGTTCCAGATGAAGAAGAAAATGAAGAGAGTGACAACGAAAAGGAAACTGAAAAGAGTGACTCCGTAACAGATTCTGGACCA
ACCTTCAACTATCTTCTTGATATGCCCCTTTGGTATTTAACCAAGGAAAAGAAAGATGAACTCTGCAGGCTAAGAAATGAAAAAGAACAA
GAGCTGGACACATTAAAAAGAAAGAGTCCATCAGATTTGTGGAAAGAAGACTTGGCTACATTTATTGAAGAATTGGAGGCTGTTGAAGCC
AAGGAAAAACAAGATGAACAAGTCGGACTTCCTGGGAAAGGGGGGAAGGCCAAGGGGAAAAAAACACAAATGGCTGAAGTTTTGCCTTCT
CCGCGTGGTCAAAGAGTCATTCCACGAATAACCATAGAAATGAAAGCAGAGGCAGAAAAGAAAAATAAAAAGAAAATTAAGAATGAAAAT
ACTGAAGGAAGCCCTCAAGAAGATGGTGTGGAACTAGAAGGCCTAAAACAAAGATTAGAAAAGAAACAGAAAAGAGAACCAGGTACAAAG
ACAAAGAAACAAACTACATTGGCATTTAAGCCAATCAAAAAAGGAAAGAAGAGAAATCCCTGGTCTGATTCAGAATCAGATAGGAGCAGT
GACGAAAGTAATTTTGATGTCCCTCCACGAGAAACAGAGCCACGGAGAGCAGCAACAAAAACAAAATTCACAATGGATTTGGATTCAGAT
GAAGATTTCTCAGATTTTGATGAAAAAACTGATGATGAAGATTTTGTCCCATCAGATGCTAGTCCACCTAAGACCAAAACTTCCCCAAAA
CTTAGTAACAAAGAACTGAAACCACAGAAAAGTGTCGTGTCAGACCTTGAAGCTGATGATGTTAAGGGCAGTGTACCACTGTCTTCAAGC
CCTCCTGCTACACATTTCCCAGATGAAACTGAAATTACAAACCCAGTTCCTAAAAAGAATGTGACAGTGAAGAAGACAGCAGCAAAAAGT
CAGTCTTCCACCTCCACTACCGGTGCCAAAAAAAGGGCTGCCCCAAAAGGAACTAAAAGGGATCCAGCTTTGAATTCTGGTGTCTCTCAA
AAGCCTGATCCTGCCAAAACCAAGAATCGCCGCAAAAGGAAGCCATCCACTTCTGATGATTCTGACTCTAATTTTGAGAAAATTGTTTCG
AAAGCAGTCACAAGCAAGAAATCCAAGGGGGAGAGTGATGACTTCCATATGGACTTTGACTCAGCTGTGGCTCCTCGGGCAAAATCTGTA
CGGGCAAAGAAACCTATAAAGTACCTGGAAGAGTCAGATGAAGATGATCTGTTTTAAAATGTGAGGCGATTATTTTAAGTAATTATCTTA
CCAAGCCCAAGACTGGTTTTAAAGTTACCTGAAGCTCTTAACTTCCTCCCCTCTGAATTTAGTTTGGGGAAGGTGTTTTTAGTACAAGAC
ATCAAAGTGAAGTAAAGCCCAAGTGTTCTTTAGCTTTTTATAATACTGTCTAAATAGTGACCATCTCATGGGCATTGTTTTCTTCTCTGC
TTTGTCTGTGTTTTGAGTCTGCTTTCTTTTGTCTTTAAAACCTGATTTTTAAGTTCTTCTGAACTGTAGAAATAGCTATCTGATCACTTC
AGCGTAAAGCAGTGTGTTTATTAACCATCCACTAAGCTAAAACTAGAGCAGTTTGATTTAAAAGTGTCACTCTTCCTCCTTTTCTACTTT
CAGTAGATATGAGATAGAGCATAATTATCTGTTTTATCTTAGTTTTATACATAATTTACCATCAGATAGAACTTTATGGTTCTAGTACAG
ATACTCTACTACACTCAGCCTCTTATGTGCCAAGTTTTTCTTTAAGCAATGAGAAATTGCTCATGTTCTTCATCTTCTCAAATCATCAGA
GGCCGAAGAAAAACACTTTGGCTGTGTCTATAACTTGACACAGTCAATAGAATGAAGAAAATTAGAGTAGTTATGTGATTATTTCAGCTC
TTGACCTGTCCCCTCTGGCTGCCTCTGAGTCTGAATCTCCCAAAGAGAGAAACCAATTTCTAAGAGGACTGGATTGCAGAAGACTCGGGG
ACAACATTTGATCCAAGATCTTAAATGTTATATTGATAACCATGCTCAGCAATGAGCTATTAGATTCATTTTGGGAAATCTCCATAATTT
CAATTTGTAAACTTTGTTAAGACCTGTCTACATTGTTATATGTGTGTGACTTGAGTAATGTTATCAACGTTTTTGTAAATATTTACTATG

>In-frame_ENST00000590078_ENST00000423485_TCGA-B6-A0IG_EZH1_chr17_40854894_-_TOP2A_chr17_38561135_length(amino acids)=863AA_start in transcript=2235_stop in transcript=4826
MEDRRQRKLLGLPEDYLYGQTTTYLTYNDFINKELILFSNSDNERSIPSMVDGLKPGQRKVLFTCFKRNDKREVKVAQLAGSVAEMSSYH
HGEMSLMMTIINLAQNFVGSNNLNLLQPIGQFGTRLHGGKDSASPRYIFTMLSSLARLLFPPKDDHTLKFLYDDNQRVEPEWYIPIIPMV
LINGAEGIGTGWSCKIPNFDVREIVNNIRRLMDGEEPLPMLPSYKNFKGTIEELAPNQYVISGEVAILNSTTIEISELPVRTWTQTYKEQ
VLEPMLNGTEKTPPLITDYREYHTDTTVKFVVKMTEEKLAEAERVGLHKVFKLQTSLTCNSMVLFDHVGCLKKYDTVLDILRDFFELRLK
YYGLRKEWLLGMLGAESAKLNNQARFILEKIDGKIIIENKPKKELIKVLIQRGYDSDPVKAWKEAQQKVPDEEENEESDNEKETEKSDSV
TDSGPTFNYLLDMPLWYLTKEKKDELCRLRNEKEQELDTLKRKSPSDLWKEDLATFIEELEAVEAKEKQDEQVGLPGKGGKAKGKKTQMA
EVLPSPRGQRVIPRITIEMKAEAEKKNKKKIKNENTEGSPQEDGVELEGLKQRLEKKQKREPGTKTKKQTTLAFKPIKKGKKRNPWSDSE
SDRSSDESNFDVPPRETEPRRAATKTKFTMDLDSDEDFSDFDEKTDDEDFVPSDASPPKTKTSPKLSNKELKPQKSVVSDLEADDVKGSV
PLSSSPPATHFPDETEITNPVPKKNVTVKKTAAKSQSSTSTTGAKKRAAPKGTKRDPALNSGVSQKPDPAKTKNRRKRKPSTSDDSDSNF

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Top

Fusion Gene PPI Analysis for EZH1-TOP2A


check button Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in

ChiPPI page.


check button Protein-protein interactors with each fusion partner protein in wild-type (BIOGRID-3.4.160)
HgeneHgene's interactorsTgeneTgene's interactors


check button - Retained PPIs in in-frame fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenStill interaction with
TgeneTOP2Achr17:40854894chr17:38561135ENST000004234851535990_999651.01532.0DNA
TgeneTOP2Achr17:40854895chr17:38561135ENST000004234851535990_999651.01532.0DNA
TgeneTOP2Achr17:40854894chr17:38561135ENST0000042348515351433_1439651.01532.0PLSCR1
TgeneTOP2Achr17:40854895chr17:38561135ENST0000042348515351433_1439651.01532.0PLSCR1


check button - Lost PPIs in in-frame fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenInteraction lost with


check button - Retained PPIs, but lost function due to frame-shift fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenInteraction lost with


Top

Related Drugs for EZH1-TOP2A


check button Drugs targeting genes involved in this fusion gene.
(DrugBank Version 5.1.8 2021-05-08)
PartnerGeneUniProtAccDrugBank IDDrug nameDrug activityDrug typeDrug status
HgeneEZH1Q92800DB12887TazemetostatInhibitorSmall moleculeApproved|Investigational
HgeneEZH1Q92800DB12887TazemetostatInhibitorSmall moleculeApproved|Investigational

Top

Related Diseases for EZH1-TOP2A


check button Diseases associated with fusion partners.
(DisGeNet 4.0)
PartnerGeneDisease IDDisease name# pubmedsSource
TgeneTOP2AC0006142Malignant neoplasm of breast4CTD_human
TgeneTOP2AC0678222Breast Carcinoma4CTD_human
TgeneTOP2AC1257931Mammary Neoplasms, Human4CTD_human
TgeneTOP2AC1458155Mammary Neoplasms4CTD_human
TgeneTOP2AC4704874Mammary Carcinoma, Human4CTD_human
TgeneTOP2AC0027819Neuroblastoma2ORPHANET
TgeneTOP2AC0235874Disease Exacerbation2CTD_human
TgeneTOP2AC2931189Neural crest tumor2ORPHANET
TgeneTOP2AC0010606Adenoid Cystic Carcinoma1CTD_human
TgeneTOP2AC0017638Glioma1CTD_human
TgeneTOP2AC0023418leukemia1CTD_human
TgeneTOP2AC0023903Liver neoplasms1CTD_human
TgeneTOP2AC0032460Polycystic Ovary Syndrome1CTD_human
TgeneTOP2AC0033578Prostatic Neoplasms1CTD_human
TgeneTOP2AC0036095Salivary Gland Neoplasms1CTD_human
TgeneTOP2AC0152013Adenocarcinoma of lung (disorder)1CTD_human
TgeneTOP2AC0206686Adrenocortical carcinoma1CTD_human
TgeneTOP2AC0220636Malignant neoplasm of salivary gland1CTD_human
TgeneTOP2AC0259783mixed gliomas1CTD_human
TgeneTOP2AC0345904Malignant neoplasm of liver1CTD_human
TgeneTOP2AC0376358Malignant neoplasm of prostate1CTD_human
TgeneTOP2AC0555198Malignant Glioma1CTD_human
TgeneTOP2AC1136382Sclerocystic Ovaries1CTD_human
TgeneTOP2AC2239176Liver carcinoma1CTD_human