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 | Fusion Gene Summary |
| Fusion Gene ORF analysis |
| Fusion Genomic Features |
| Fusion Protein Features |
| Fusion Gene Sequence |
| Fusion Gene PPI analysis |
| Related Drugs |
| Related Diseases |
Fusion gene:FTO-ZNF536 (FusionGDB2 ID:31615) |
Fusion Gene Summary for FTO-ZNF536 |
 Fusion gene summary |
| Fusion gene information | Fusion gene name: FTO-ZNF536 | Fusion gene ID: 31615 | Hgene | Tgene | Gene symbol | FTO | ZNF536 | Gene ID | 79068 | 9745 |
| Gene name | FTO alpha-ketoglutarate dependent dioxygenase | zinc finger protein 536 | |
| Synonyms | ALKBH9|BMIQ14|GDFD | - | |
| Cytomap | 16q12.2 | 19q12 | |
| Type of gene | protein-coding | protein-coding | |
| Description | alpha-ketoglutarate-dependent dioxygenase FTOAlkB homolog 9U6 small nuclear RNA (2'-O-methyladenosine-N(6)-)-demethylase FTOU6 small nuclear RNA N(6)-methyladenosine-demethylase FTOfat mass and obesity associatedfat mass and obesity-associated protei | zinc finger protein 536 | |
| Modification date | 20200329 | 20200313 | |
| UniProtAcc | Q9C0B1 | . | |
| Ensembl transtripts involved in fusion gene | ENST00000471389, ENST00000394647, ENST00000431610, ENST00000460382, ENST00000463855, ENST00000472835, | ENST00000355537, ENST00000590564, | |
| Fusion gene scores | * DoF score | 5 X 2 X 5=50 | 21 X 13 X 13=3549 |
| # samples | 5 | 25 | |
| ** MAII score | log2(5/50*10)=0 | log2(25/3549*10)=-3.82741257439886 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0 | |
| Context | PubMed: FTO [Title/Abstract] AND ZNF536 [Title/Abstract] AND fusion [Title/Abstract] | ||
| Most frequent breakpoint | FTO(53738141)-ZNF536(31025754), # samples:1 | ||
| Anticipated loss of major functional domain due to fusion event. | FTO-ZNF536 seems lost the major protein functional domain in Hgene partner, which is a essential gene due to the frame-shifted ORF. FTO-ZNF536 seems lost the major protein functional domain in Tgene partner, which is a transcription factor due to the frame-shifted ORF. FTO-ZNF536 seems lost the major protein functional domain in Tgene partner, which is a essential gene due to the frame-shifted ORF. | ||
| * DoF score (Degree of Frequency) = # partners X # break points X # cancer types ** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10) |
| Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez |
| Partner | Gene | GO ID | GO term | PubMed ID |
| Hgene | FTO | GO:0006307 | DNA dealkylation involved in DNA repair | 18775698|20376003 |
| Hgene | FTO | GO:0035552 | oxidative single-stranded DNA demethylation | 18775698|20376003 |
| Hgene | FTO | GO:0035553 | oxidative single-stranded RNA demethylation | 18775698|22002720|25452335|26457839|28002401|30197295 |
| Hgene | FTO | GO:0042245 | RNA repair | 18775698 |
| Hgene | FTO | GO:0061157 | mRNA destabilization | 28002401|30197295 |
| Hgene | FTO | GO:0070989 | oxidative demethylation | 18775698 |
| Hgene | FTO | GO:0080111 | DNA demethylation | 18775698 |
| Tgene | ZNF536 | GO:0000122 | negative regulation of transcription by RNA polymerase II | 19398580 |
| Fusion gene breakpoints across FTO (5'-gene) * Click on the image to open the UCSC genome browser with custom track showing this image in a new window. |
| Fusion gene breakpoints across ZNF536 (3'-gene) * Click on the image to open the UCSC genome browser with custom track showing this image in a new window. |
| Fusion gene information from two resources (ChiTars 5.0 and ChimerDB 4.0) * All genome coordinats were lifted-over on hg19. * Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser. |
| Source | Disease | Sample | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand |
| ChimerDB4 | HNSC | TCGA-CN-5364-01A | FTO | chr16 | 53738141 | - | ZNF536 | chr19 | 31025754 | + |
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Fusion Gene ORF analysis for FTO-ZNF536 |
| Open reading frame (ORF) analsis of fusion genes based on Ensembl gene isoform structure. * Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser. |
| ORF | Henst | Tenst | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand |
| Frame-shift | ENST00000471389 | ENST00000355537 | FTO | chr16 | 53738141 | - | ZNF536 | chr19 | 31025754 | + |
| 5CDS-intron | ENST00000471389 | ENST00000590564 | FTO | chr16 | 53738141 | - | ZNF536 | chr19 | 31025754 | + |
| 5UTR-3CDS | ENST00000394647 | ENST00000355537 | FTO | chr16 | 53738141 | - | ZNF536 | chr19 | 31025754 | + |
| 5UTR-intron | ENST00000394647 | ENST00000590564 | FTO | chr16 | 53738141 | - | ZNF536 | chr19 | 31025754 | + |
| intron-3CDS | ENST00000431610 | ENST00000355537 | FTO | chr16 | 53738141 | - | ZNF536 | chr19 | 31025754 | + |
| intron-intron | ENST00000431610 | ENST00000590564 | FTO | chr16 | 53738141 | - | ZNF536 | chr19 | 31025754 | + |
| intron-3CDS | ENST00000460382 | ENST00000355537 | FTO | chr16 | 53738141 | - | ZNF536 | chr19 | 31025754 | + |
| intron-intron | ENST00000460382 | ENST00000590564 | FTO | chr16 | 53738141 | - | ZNF536 | chr19 | 31025754 | + |
| intron-3CDS | ENST00000463855 | ENST00000355537 | FTO | chr16 | 53738141 | - | ZNF536 | chr19 | 31025754 | + |
| intron-intron | ENST00000463855 | ENST00000590564 | FTO | chr16 | 53738141 | - | ZNF536 | chr19 | 31025754 | + |
| intron-3CDS | ENST00000472835 | ENST00000355537 | FTO | chr16 | 53738141 | - | ZNF536 | chr19 | 31025754 | + |
| intron-intron | ENST00000472835 | ENST00000590564 | FTO | chr16 | 53738141 | - | ZNF536 | chr19 | 31025754 | + |
| ORFfinder result based on the fusion transcript sequence of in-frame fusion genes. |
| Henst | Tenst | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand | Seq length (transcript) | BP loci (transcript) | Predicted start (transcript) | Predicted stop (transcript) | Seq length (amino acids) |
| DeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated. |
| Henst | Tenst | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand | No-coding score | Coding score |
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Fusion Genomic Features for FTO-ZNF536 |
| FusionAI prediction of the potential fusion gene breakpoint based on the pre-mature RNA sequence context (+/- 5kb of individual partner genes, total 20kb length sequence). FusionAI is a fusion gene breakpoint classifier based on convolutional neural network by comparing the fusion positive and negative sequence context of ~ 20K fusion gene data. From here, we can have the relative potentency of the 20K genomic sequence how individual sequnce will be likely used as the gene fusion breakpoints. |
| Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand | 1-p | p (fusion gene breakpoint) |
| Distribution of 44 human genomic features loci across 20kb length fusion breakpoint regions. We integrated a total of 44 different types of human genomic feature loci information across five big categories including virus integration sites, repeats, structural variants, chromatin states, and gene expression regulation. More details are in help page. |
| Distribution of 44 human genomic features loci across 20kb length fusion breakpoint regions that are ovelapped with the top 1% feature importance score regions. More details are in help page. |
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Fusion Protein Features for FTO-ZNF536 |
| Go to FGviewer for the breakpoints of :-: - FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels. |
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| Main function of each fusion partner protein. (from UniProt) |
| Hgene | Tgene |
| FTO | . |
| FUNCTION: RNA demethylase that mediates oxidative demethylation of different RNA species, such as mRNAs, tRNAs and snRNAs, and acts as a regulator of fat mass, adipogenesis and energy homeostasis (PubMed:22002720, PubMed:26458103, PubMed:28002401, PubMed:30197295, PubMed:26457839, PubMed:25452335). Specifically demethylates N(6)-methyladenosine (m6A) RNA, the most prevalent internal modification of messenger RNA (mRNA) in higher eukaryotes (PubMed:22002720, PubMed:26458103, PubMed:30197295, PubMed:26457839, PubMed:25452335). M6A demethylation by FTO affects mRNA expression and stability (PubMed:30197295). Also able to demethylate m6A in U6 small nuclear RNA (snRNA) (PubMed:30197295). Mediates demethylation of N(6),2'-O-dimethyladenosine cap (m6A(m)), by demethylating the N(6)-methyladenosine at the second transcribed position of mRNAs and U6 snRNA (PubMed:28002401, PubMed:30197295). Demethylation of m6A(m) in the 5'-cap by FTO affects mRNA stability by promoting susceptibility to decapping (PubMed:28002401). Also acts as a tRNA demethylase by removing N(1)-methyladenine from various tRNAs (PubMed:30197295). Has no activity towards 1-methylguanine (PubMed:20376003). Has no detectable activity towards double-stranded DNA (PubMed:20376003). Also able to repair alkylated DNA and RNA by oxidative demethylation: demethylates single-stranded RNA containing 3-methyluracil, single-stranded DNA containing 3-methylthymine and has low demethylase activity towards single-stranded DNA containing 1-methyladenine or 3-methylcytosine (PubMed:18775698, PubMed:20376003). Ability to repair alkylated DNA and RNA is however unsure in vivo (PubMed:18775698, PubMed:20376003). Involved in the regulation of fat mass, adipogenesis and body weight, thereby contributing to the regulation of body size and body fat accumulation (PubMed:18775698, PubMed:20376003). Involved in the regulation of thermogenesis and the control of adipocyte differentiation into brown or white fat cells (PubMed:26287746). Regulates activity of the dopaminergic midbrain circuitry via its ability to demethylate m6A in mRNAs (By similarity). Plays an oncogenic role in a number of acute myeloid leukemias by enhancing leukemic oncogene-mediated cell transformation: acts by mediating m6A demethylation of target transcripts such as MYC, CEBPA, ASB2 and RARA, leading to promote their expression (PubMed:28017614, PubMed:29249359). {ECO:0000250|UniProtKB:Q8BGW1, ECO:0000269|PubMed:18775698, ECO:0000269|PubMed:20376003, ECO:0000269|PubMed:22002720, ECO:0000269|PubMed:25452335, ECO:0000269|PubMed:26287746, ECO:0000269|PubMed:26457839, ECO:0000269|PubMed:26458103, ECO:0000269|PubMed:28002401, ECO:0000269|PubMed:28017614, ECO:0000269|PubMed:29249359, ECO:0000269|PubMed:30197295}. | FUNCTION: Might normally function as a transcriptional repressor. EWS-fusion-proteins (EFPS) may play a role in the tumorigenic process. They may disturb gene expression by mimicking, or interfering with the normal function of CTD-POLII within the transcription initiation complex. They may also contribute to an aberrant activation of the fusion protein target genes. |
| Retention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at * Minus value of BPloci means that the break pointn is located before the CDS. |
| - In-frame and retained protein feature among the 13 regional features. |
| Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Protein feature | Protein feature note |
| - In-frame and not-retained protein feature among the 13 regional features. |
| Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Protein feature | Protein feature note |
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Fusion Gene Sequence for FTO-ZNF536 |
| For in-frame fusion transcripts, we provide the fusion transcript sequences and fusion amino acid sequences. To have fusion amino acid sequence, we ran ORFfinder and chose the longest ORF among the all predicted ones. |
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Fusion Gene PPI Analysis for FTO-ZNF536 |
| Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in |
| Protein-protein interactors with each fusion partner protein in wild-type (BIOGRID-3.4.160) |
| Hgene | Hgene's interactors | Tgene | Tgene's interactors |
| - Retained PPIs in in-frame fusion. |
| Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Still interaction with |
| - Lost PPIs in in-frame fusion. |
| Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Interaction lost with |
| - Retained PPIs, but lost function due to frame-shift fusion. |
| Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Interaction lost with |
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Related Drugs for FTO-ZNF536 |
| Drugs targeting genes involved in this fusion gene. (DrugBank Version 5.1.8 2021-05-08) |
| Partner | Gene | UniProtAcc | DrugBank ID | Drug name | Drug activity | Drug type | Drug status |
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Related Diseases for FTO-ZNF536 |
| Diseases associated with fusion partners. (DisGeNet 4.0) |
| Partner | Gene | Disease ID | Disease name | # pubmeds | Source |
| Hgene | FTO | C0028754 | Obesity | 6 | CTD_human |
| Hgene | FTO | C2752001 | Growth Retardation, Developmental Delay, Coarse Facies, And Early Death | 4 | CTD_human;GENOMICS_ENGLAND;ORPHANET;UNIPROT |
| Hgene | FTO | C0001973 | Alcoholic Intoxication, Chronic | 3 | PSYGENET |
| Hgene | FTO | C0011581 | Depressive disorder | 3 | PSYGENET |
| Hgene | FTO | C0005910 | Body Weight | 2 | CTD_human |
| Hgene | FTO | C0011570 | Mental Depression | 2 | PSYGENET |
| Hgene | FTO | C0006142 | Malignant neoplasm of breast | 1 | CTD_human |
| Hgene | FTO | C0011860 | Diabetes Mellitus, Non-Insulin-Dependent | 1 | CTD_human |
| Hgene | FTO | C0025202 | melanoma | 1 | CTD_human |
| Hgene | FTO | C0043094 | Weight Gain | 1 | CTD_human |
| Hgene | FTO | C0525045 | Mood Disorders | 1 | PSYGENET |
| Hgene | FTO | C0678222 | Breast Carcinoma | 1 | CTD_human |
| Hgene | FTO | C1257931 | Mammary Neoplasms, Human | 1 | CTD_human |
| Hgene | FTO | C1458155 | Mammary Neoplasms | 1 | CTD_human |
| Hgene | FTO | C2362324 | Pediatric Obesity | 1 | CTD_human |
| Hgene | FTO | C4317171 | Adolescent Obesity | 1 | CTD_human |
| Hgene | FTO | C4521075 | Childhood Overweight | 1 | CTD_human |
| Hgene | FTO | C4553478 | Infantile Obesity | 1 | CTD_human |
| Hgene | FTO | C4704874 | Mammary Carcinoma, Human | 1 | CTD_human |
| Hgene | FTO | C4704955 | Infant Overweight | 1 | CTD_human |
| Hgene | FTO | C4704956 | Adolescent Overweight | 1 | CTD_human |
| Tgene | ZNF536 | C0007134 | Renal Cell Carcinoma | 1 | CTD_human |
| Tgene | ZNF536 | C0279702 | Conventional (Clear Cell) Renal Cell Carcinoma | 1 | CTD_human |
| Tgene | ZNF536 | C1266042 | Chromophobe Renal Cell Carcinoma | 1 | CTD_human |
| Tgene | ZNF536 | C1266043 | Sarcomatoid Renal Cell Carcinoma | 1 | CTD_human |
| Tgene | ZNF536 | C1266044 | Collecting Duct Carcinoma of the Kidney | 1 | CTD_human |
| Tgene | ZNF536 | C1306837 | Papillary Renal Cell Carcinoma | 1 | CTD_human |
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