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 | Fusion Gene Summary |
| Fusion Gene ORF analysis |
| Fusion Genomic Features |
| Fusion Protein Features |
| Fusion Gene Sequence |
| Fusion Gene PPI analysis |
| Related Drugs |
| Related Diseases |
Fusion gene:FUS-PYCARD (FusionGDB2 ID:31710) |
Fusion Gene Summary for FUS-PYCARD |
 Fusion gene summary |
| Fusion gene information | Fusion gene name: FUS-PYCARD | Fusion gene ID: 31710 | Hgene | Tgene | Gene symbol | FUS | PYCARD | Gene ID | 2521 | 29108 |
| Gene name | FUS RNA binding protein | PYD and CARD domain containing | |
| Synonyms | ALS6|ETM4|FUS1|HNRNPP2|POMP75|TLS | ASC|CARD5|TMS|TMS-1|TMS1 | |
| Cytomap | 16p11.2 | 16p11.2 | |
| Type of gene | protein-coding | protein-coding | |
| Description | RNA-binding protein FUS75 kDa DNA-pairing proteinfus-like proteinfused in sarcomafusion gene in myxoid liposarcomaheterogeneous nuclear ribonucleoprotein P2oncogene FUSoncogene TLStranslocated in liposarcoma protein | apoptosis-associated speck-like protein containing a CARDcaspase recruitment domain-containing protein 5target of methylation-induced silencing 1 | |
| Modification date | 20200329 | 20200313 | |
| UniProtAcc | P35637 | . | |
| Ensembl transtripts involved in fusion gene | ENST00000254108, ENST00000380244, ENST00000568685, ENST00000474990, | ENST00000247470, ENST00000350605, | |
| Fusion gene scores | * DoF score | 37 X 37 X 16=21904 | 1 X 1 X 1=1 |
| # samples | 44 | 1 | |
| ** MAII score | log2(44/21904*10)=-5.63754701773324 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0 | log2(1/1*10)=3.32192809488736 | |
| Context | PubMed: FUS [Title/Abstract] AND PYCARD [Title/Abstract] AND fusion [Title/Abstract] | ||
| Most frequent breakpoint | FUS(31193743)-PYCARD(31213524), # samples:2 | ||
| Anticipated loss of major functional domain due to fusion event. | FUS-PYCARD seems lost the major protein functional domain in Hgene partner, which is a CGC due to the frame-shifted ORF. FUS-PYCARD seems lost the major protein functional domain in Hgene partner, which is a tumor suppressor due to the frame-shifted ORF. FUS-PYCARD seems lost the major protein functional domain in Hgene partner, which is a essential gene due to the frame-shifted ORF. FUS-PYCARD seems lost the major protein functional domain in Tgene partner, which is a tumor suppressor due to the frame-shifted ORF. FUS-PYCARD seems lost the major protein functional domain in Tgene partner, which is a essential gene due to the frame-shifted ORF. | ||
| * DoF score (Degree of Frequency) = # partners X # break points X # cancer types ** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10) |
| Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez |
| Partner | Gene | GO ID | GO term | PubMed ID |
| Hgene | FUS | GO:0006355 | regulation of transcription, DNA-templated | 26124092 |
| Hgene | FUS | GO:0006357 | regulation of transcription by RNA polymerase II | 25453086 |
| Hgene | FUS | GO:0008380 | RNA splicing | 26124092 |
| Hgene | FUS | GO:0043484 | regulation of RNA splicing | 25453086|27731383 |
| Hgene | FUS | GO:0048255 | mRNA stabilization | 27378374 |
| Hgene | FUS | GO:0051260 | protein homooligomerization | 25453086 |
| Hgene | FUS | GO:1905168 | positive regulation of double-strand break repair via homologous recombination | 10567410 |
| Tgene | PYCARD | GO:0002218 | activation of innate immune response | 21575908 |
| Tgene | PYCARD | GO:0033209 | tumor necrosis factor-mediated signaling pathway | 12656673 |
| Tgene | PYCARD | GO:0043065 | positive regulation of apoptotic process | 12646168 |
| Tgene | PYCARD | GO:0043124 | negative regulation of I-kappaB kinase/NF-kappaB signaling | 12486103 |
| Tgene | PYCARD | GO:0043280 | positive regulation of cysteine-type endopeptidase activity involved in apoptotic process | 15030775 |
| Tgene | PYCARD | GO:0050718 | positive regulation of interleukin-1 beta secretion | 15030775 |
| Tgene | PYCARD | GO:0051091 | positive regulation of DNA-binding transcription factor activity | 19494289 |
| Tgene | PYCARD | GO:0051092 | positive regulation of NF-kappaB transcription factor activity | 12646168|12656673 |
| Tgene | PYCARD | GO:0051260 | protein homooligomerization | 24531343 |
| Tgene | PYCARD | GO:0051607 | defense response to virus | 21575908 |
| Tgene | PYCARD | GO:0071222 | cellular response to lipopolysaccharide | 12486103 |
| Tgene | PYCARD | GO:0071347 | cellular response to interleukin-1 | 12486103 |
| Tgene | PYCARD | GO:0071356 | cellular response to tumor necrosis factor | 12486103 |
| Tgene | PYCARD | GO:0071901 | negative regulation of protein serine/threonine kinase activity | 12486103 |
| Tgene | PYCARD | GO:0090200 | positive regulation of release of cytochrome c from mitochondria | 14730312 |
| Tgene | PYCARD | GO:2001056 | positive regulation of cysteine-type endopeptidase activity | 19158676 |
| Tgene | PYCARD | GO:2001238 | positive regulation of extrinsic apoptotic signaling pathway | 16964285 |
| Tgene | PYCARD | GO:2001242 | regulation of intrinsic apoptotic signaling pathway | 14730312 |
| Fusion gene breakpoints across FUS (5'-gene) * Click on the image to open the UCSC genome browser with custom track showing this image in a new window. |
| Fusion gene breakpoints across PYCARD (3'-gene) * Click on the image to open the UCSC genome browser with custom track showing this image in a new window. |
| Fusion gene information from two resources (ChiTars 5.0 and ChimerDB 4.0) * All genome coordinats were lifted-over on hg19. * Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser. |
| Source | Disease | Sample | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand |
| ChimerDB4 | COAD | TCGA-F4-6809-01A | FUS | chr16 | 31193743 | + | PYCARD | chr16 | 31213524 | - |
| ChimerDB4 | COAD | TCGA-F4-6809-01A | FUS | chr16 | 31193743 | + | PYCARD | chr16 | 31213524 | - |
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Fusion Gene ORF analysis for FUS-PYCARD |
| Open reading frame (ORF) analsis of fusion genes based on Ensembl gene isoform structure. * Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser. |
| ORF | Henst | Tenst | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand |
| Frame-shift | ENST00000254108 | ENST00000247470 | FUS | chr16 | 31193743 | + | PYCARD | chr16 | 31213524 | - |
| 5CDS-intron | ENST00000254108 | ENST00000350605 | FUS | chr16 | 31193743 | + | PYCARD | chr16 | 31213524 | - |
| Frame-shift | ENST00000380244 | ENST00000247470 | FUS | chr16 | 31193743 | + | PYCARD | chr16 | 31213524 | - |
| 5CDS-intron | ENST00000380244 | ENST00000350605 | FUS | chr16 | 31193743 | + | PYCARD | chr16 | 31213524 | - |
| Frame-shift | ENST00000568685 | ENST00000247470 | FUS | chr16 | 31193743 | + | PYCARD | chr16 | 31213524 | - |
| 5CDS-intron | ENST00000568685 | ENST00000350605 | FUS | chr16 | 31193743 | + | PYCARD | chr16 | 31213524 | - |
| intron-3CDS | ENST00000474990 | ENST00000247470 | FUS | chr16 | 31193743 | + | PYCARD | chr16 | 31213524 | - |
| intron-intron | ENST00000474990 | ENST00000350605 | FUS | chr16 | 31193743 | + | PYCARD | chr16 | 31213524 | - |
| ORFfinder result based on the fusion transcript sequence of in-frame fusion genes. |
| Henst | Tenst | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand | Seq length (transcript) | BP loci (transcript) | Predicted start (transcript) | Predicted stop (transcript) | Seq length (amino acids) |
| DeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated. |
| Henst | Tenst | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand | No-coding score | Coding score |
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Fusion Genomic Features for FUS-PYCARD |
| FusionAI prediction of the potential fusion gene breakpoint based on the pre-mature RNA sequence context (+/- 5kb of individual partner genes, total 20kb length sequence). FusionAI is a fusion gene breakpoint classifier based on convolutional neural network by comparing the fusion positive and negative sequence context of ~ 20K fusion gene data. From here, we can have the relative potentency of the 20K genomic sequence how individual sequnce will be likely used as the gene fusion breakpoints. |
| Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand | 1-p | p (fusion gene breakpoint) |
| Distribution of 44 human genomic features loci across 20kb length fusion breakpoint regions. We integrated a total of 44 different types of human genomic feature loci information across five big categories including virus integration sites, repeats, structural variants, chromatin states, and gene expression regulation. More details are in help page. |
| Distribution of 44 human genomic features loci across 20kb length fusion breakpoint regions that are ovelapped with the top 1% feature importance score regions. More details are in help page. |
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Fusion Protein Features for FUS-PYCARD |
| Go to FGviewer for the breakpoints of :-: - FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels. |
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| Main function of each fusion partner protein. (from UniProt) |
| Hgene | Tgene |
| FUS | . |
| FUNCTION: DNA/RNA-binding protein that plays a role in various cellular processes such as transcription regulation, RNA splicing, RNA transport, DNA repair and damage response (PubMed:27731383). Binds to nascent pre-mRNAs and acts as a molecular mediator between RNA polymerase II and U1 small nuclear ribonucleoprotein thereby coupling transcription and splicing (PubMed:26124092). Binds also its own pre-mRNA and autoregulates its expression; this autoregulation mechanism is mediated by non-sense-mediated decay (PubMed:24204307). Plays a role in DNA repair mechanisms by promoting D-loop formation and homologous recombination during DNA double-strand break repair (PubMed:10567410). In neuronal cells, plays crucial roles in dendritic spine formation and stability, RNA transport, mRNA stability and synaptic homeostasis (By similarity). {ECO:0000250|UniProtKB:P56959, ECO:0000269|PubMed:10567410, ECO:0000269|PubMed:24204307, ECO:0000269|PubMed:26124092, ECO:0000269|PubMed:27731383}. | FUNCTION: Might normally function as a transcriptional repressor. EWS-fusion-proteins (EFPS) may play a role in the tumorigenic process. They may disturb gene expression by mimicking, or interfering with the normal function of CTD-POLII within the transcription initiation complex. They may also contribute to an aberrant activation of the fusion protein target genes. |
| Retention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at * Minus value of BPloci means that the break pointn is located before the CDS. |
| - In-frame and retained protein feature among the 13 regional features. |
| Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Protein feature | Protein feature note |
| - In-frame and not-retained protein feature among the 13 regional features. |
| Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Protein feature | Protein feature note |
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Fusion Gene Sequence for FUS-PYCARD |
| For in-frame fusion transcripts, we provide the fusion transcript sequences and fusion amino acid sequences. To have fusion amino acid sequence, we ran ORFfinder and chose the longest ORF among the all predicted ones. |
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Fusion Gene PPI Analysis for FUS-PYCARD |
| Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in |
| Protein-protein interactors with each fusion partner protein in wild-type (BIOGRID-3.4.160) |
| Hgene | Hgene's interactors | Tgene | Tgene's interactors |
| - Retained PPIs in in-frame fusion. |
| Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Still interaction with |
| - Lost PPIs in in-frame fusion. |
| Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Interaction lost with |
| - Retained PPIs, but lost function due to frame-shift fusion. |
| Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Interaction lost with |
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Related Drugs for FUS-PYCARD |
| Drugs targeting genes involved in this fusion gene. (DrugBank Version 5.1.8 2021-05-08) |
| Partner | Gene | UniProtAcc | DrugBank ID | Drug name | Drug activity | Drug type | Drug status |
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Related Diseases for FUS-PYCARD |
| Diseases associated with fusion partners. (DisGeNet 4.0) |
| Partner | Gene | Disease ID | Disease name | # pubmeds | Source |
| Hgene | FUS | C1842675 | AMYOTROPHIC LATERAL SCLEROSIS 6 (disorder) | 5 | UNIPROT |
| Hgene | FUS | C3468114 | Juvenile amyotrophic lateral sclerosis | 5 | ORPHANET |
| Hgene | FUS | C0002736 | Amyotrophic Lateral Sclerosis | 2 | CTD_human;ORPHANET |
| Hgene | FUS | C0206634 | Liposarcoma, Myxoid | 2 | CTD_human;ORPHANET |
| Hgene | FUS | C0393554 | Amyotrophic Lateral Sclerosis With Dementia | 1 | CTD_human |
| Hgene | FUS | C0497327 | Dementia | 1 | GENOMICS_ENGLAND |
| Hgene | FUS | C0543859 | Amyotrophic Lateral Sclerosis, Guam Form | 1 | CTD_human |
| Hgene | FUS | C3539195 | TREMOR, HEREDITARY ESSENTIAL, 4 | 1 | CTD_human;UNIPROT |
| Hgene | FUS | C3888102 | Frontotemporal Dementia With Motor Neuron Disease | 1 | ORPHANET |
| Tgene | PYCARD | C0022658 | Kidney Diseases | 2 | CTD_human |
| Tgene | PYCARD | C0001418 | Adenocarcinoma | 1 | CTD_human |
| Tgene | PYCARD | C0001925 | Albuminuria | 1 | CTD_human |
| Tgene | PYCARD | C0006663 | Calcinosis | 1 | CTD_human |
| Tgene | PYCARD | C0007131 | Non-Small Cell Lung Carcinoma | 1 | CTD_human |
| Tgene | PYCARD | C0007137 | Squamous cell carcinoma | 1 | CTD_human |
| Tgene | PYCARD | C0009402 | Colorectal Carcinoma | 1 | CTD_human |
| Tgene | PYCARD | C0009404 | Colorectal Neoplasms | 1 | CTD_human |
| Tgene | PYCARD | C0018824 | Heart valve disease | 1 | CTD_human |
| Tgene | PYCARD | C0023893 | Liver Cirrhosis, Experimental | 1 | CTD_human |
| Tgene | PYCARD | C0024121 | Lung Neoplasms | 1 | CTD_human |
| Tgene | PYCARD | C0024623 | Malignant neoplasm of stomach | 1 | CTD_human |
| Tgene | PYCARD | C0038356 | Stomach Neoplasms | 1 | CTD_human |
| Tgene | PYCARD | C0043094 | Weight Gain | 1 | CTD_human |
| Tgene | PYCARD | C0205641 | Adenocarcinoma, Basal Cell | 1 | CTD_human |
| Tgene | PYCARD | C0205642 | Adenocarcinoma, Oxyphilic | 1 | CTD_human |
| Tgene | PYCARD | C0205643 | Carcinoma, Cribriform | 1 | CTD_human |
| Tgene | PYCARD | C0205644 | Carcinoma, Granular Cell | 1 | CTD_human |
| Tgene | PYCARD | C0205645 | Adenocarcinoma, Tubular | 1 | CTD_human |
| Tgene | PYCARD | C0206704 | Carcinoma, Large Cell | 1 | CTD_human |
| Tgene | PYCARD | C0242379 | Malignant neoplasm of lung | 1 | CTD_human |
| Tgene | PYCARD | C0262584 | Carcinoma, Small Cell | 1 | CTD_human |
| Tgene | PYCARD | C0263628 | Tumoral calcinosis | 1 | CTD_human |
| Tgene | PYCARD | C0345967 | Malignant mesothelioma | 1 | CTD_human |
| Tgene | PYCARD | C0521174 | Microcalcification | 1 | CTD_human |
| Tgene | PYCARD | C0598608 | Hyperhomocysteinemia | 1 | CTD_human |
| Tgene | PYCARD | C1708349 | Hereditary Diffuse Gastric Cancer | 1 | CTD_human |
| Tgene | PYCARD | C2239176 | Liver carcinoma | 1 | CTD_human |
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