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Center for Computational Systems Medicine
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Fusion Gene Summary

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Fusion Gene ORF analysis

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Fusion Genomic Features

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Fusion Protein Features

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Fusion Gene Sequence

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Fusion Gene PPI analysis

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Fusion gene:GNAS-SMAP2 (FusionGDB2 ID:33528)

Fusion Gene Summary for GNAS-SMAP2

check button Fusion gene summary
Fusion gene informationFusion gene name: GNAS-SMAP2
Fusion gene ID: 33528
HgeneTgene
Gene symbol

GNAS

SMAP2

Gene ID

2778

64744

Gene nameGNAS complex locussmall ArfGAP2
SynonymsAHO|C20orf45|GNAS1|GPSA|GSA|GSP|NESP|PITA3|POH|SCG6|SgVISMAP1L
Cytomap

20q13.32

1p34.2

Type of geneprotein-codingprotein-coding
Descriptionprotein ALEXprotein GNASprotein SCG6 (secretogranin VI)G protein subunit alpha Sadenylate cyclase-stimulating G alpha proteinalternative gene product encoded by XL-exonextra large alphas proteinguanine nucleotide binding protein (G protein), alpha stromal membrane-associated protein 2stromal membrane-associated GTPase-activating protein 2
Modification date2020032920200313
UniProtAcc

P63092

.
Ensembl transtripts involved in fusion geneENST00000313949, ENST00000371098, 
ENST00000371075, ENST00000371100, 
ENST00000371099, ENST00000371102, 
ENST00000464624, ENST00000306120, 
ENST00000371095, ENST00000371085, 
ENST00000354359, ENST00000265620, 
ENST00000306090, ENST00000371081, 
ENST00000539317, ENST00000487871, 
Fusion gene scores* DoF score44 X 25 X 16=176007 X 7 X 4=196
# samples 517
** MAII scorelog2(51/17600*10)=-5.10893437155316
possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs).
DoF>8 and MAII<0		log2(7/196*10)=-1.48542682717024
possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs).
DoF>8 and MAII<0
Context

PubMed: GNAS [Title/Abstract] AND SMAP2 [Title/Abstract] AND fusion [Title/Abstract]

Most frequent breakpointGNAS(57486241)-SMAP2(40878688), # samples:1
Anticipated loss of major functional domain due to fusion event.GNAS-SMAP2 seems lost the major protein functional domain in Hgene partner, which is a CGC due to the frame-shifted ORF.
GNAS-SMAP2 seems lost the major protein functional domain in Hgene partner, which is a cell metabolism gene due to the frame-shifted ORF.
* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

check button Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez
PartnerGeneGO IDGO termPubMed ID

check buttonFusion gene breakpoints across GNAS (5'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

check buttonFusion gene breakpoints across SMAP2 (3'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

check button Fusion gene information from two resources (ChiTars 5.0 and ChimerDB 4.0)
* All genome coordinats were lifted-over on hg19.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.
SourceDiseaseSampleHgeneHchrHbpHstrandTgeneTchrTbpTstrand
ChimerDB4HNSCTCGA-H7-7774-01AGNASchr20

57486241

-SMAP2chr1

40878688

+


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Fusion Gene ORF analysis for GNAS-SMAP2

check button Open reading frame (ORF) analsis of fusion genes based on Ensembl gene isoform structure.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.
ORFHenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrand
Frame-shiftENST00000313949ENST00000539317GNASchr20

57486241

-SMAP2chr1

40878688

+
5CDS-intronENST00000313949ENST00000487871GNASchr20

57486241

-SMAP2chr1

40878688

+
intron-3CDSENST00000371098ENST00000539317GNASchr20

57486241

-SMAP2chr1

40878688

+
intron-intronENST00000371098ENST00000487871GNASchr20

57486241

-SMAP2chr1

40878688

+
Frame-shiftENST00000371075ENST00000539317GNASchr20

57486241

-SMAP2chr1

40878688

+
5CDS-intronENST00000371075ENST00000487871GNASchr20

57486241

-SMAP2chr1

40878688

+
In-frameENST00000371100ENST00000539317GNASchr20

57486241

-SMAP2chr1

40878688

+
5CDS-intronENST00000371100ENST00000487871GNASchr20

57486241

-SMAP2chr1

40878688

+
intron-3CDSENST00000371099ENST00000539317GNASchr20

57486241

-SMAP2chr1

40878688

+
intron-intronENST00000371099ENST00000487871GNASchr20

57486241

-SMAP2chr1

40878688

+
In-frameENST00000371102ENST00000539317GNASchr20

57486241

-SMAP2chr1

40878688

+
5CDS-intronENST00000371102ENST00000487871GNASchr20

57486241

-SMAP2chr1

40878688

+
Frame-shiftENST00000464624ENST00000539317GNASchr20

57486241

-SMAP2chr1

40878688

+
5CDS-intronENST00000464624ENST00000487871GNASchr20

57486241

-SMAP2chr1

40878688

+
intron-3CDSENST00000306120ENST00000539317GNASchr20

57486241

-SMAP2chr1

40878688

+
intron-intronENST00000306120ENST00000487871GNASchr20

57486241

-SMAP2chr1

40878688

+
In-frameENST00000371095ENST00000539317GNASchr20

57486241

-SMAP2chr1

40878688

+
5CDS-intronENST00000371095ENST00000487871GNASchr20

57486241

-SMAP2chr1

40878688

+
In-frameENST00000371085ENST00000539317GNASchr20

57486241

-SMAP2chr1

40878688

+
5CDS-intronENST00000371085ENST00000487871GNASchr20

57486241

-SMAP2chr1

40878688

+
In-frameENST00000354359ENST00000539317GNASchr20

57486241

-SMAP2chr1

40878688

+
5CDS-intronENST00000354359ENST00000487871GNASchr20

57486241

-SMAP2chr1

40878688

+
In-frameENST00000265620ENST00000539317GNASchr20

57486241

-SMAP2chr1

40878688

+
5CDS-intronENST00000265620ENST00000487871GNASchr20

57486241

-SMAP2chr1

40878688

+
In-frameENST00000306090ENST00000539317GNASchr20

57486241

-SMAP2chr1

40878688

+
5CDS-intronENST00000306090ENST00000487871GNASchr20

57486241

-SMAP2chr1

40878688

+
intron-3CDSENST00000371081ENST00000539317GNASchr20

57486241

-SMAP2chr1

40878688

+
intron-intronENST00000371081ENST00000487871GNASchr20

57486241

-SMAP2chr1

40878688

+

check buttonORFfinder result based on the fusion transcript sequence of in-frame fusion genes.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandSeq length
(transcript)		BP loci
(transcript)		Predicted start
(transcript)		Predicted stop
(transcript)		Seq length
(amino acids)
ENST00000371100GNASchr2057486241-ENST00000539317SMAP2chr140878688+5076402953136651044
ENST00000371102GNASchr2057486241-ENST00000539317SMAP2chr140878688+44853438330741023
ENST00000371095GNASchr2057486241-ENST00000539317SMAP2chr140878688+2978193115822527
ENST00000371085GNASchr2057486241-ENST00000539317SMAP2chr140878688+3019197216231541
ENST00000354359GNASchr2057486241-ENST00000539317SMAP2chr140878688+3022197516261542
ENST00000265620GNASchr2057486241-ENST00000539317SMAP2chr140878688+2913186615170506
ENST00000306090GNASchr2057486241-ENST00000539317SMAP2chr140878688+2689164212991433

check buttonDeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandNo-coding scoreCoding score
ENST00000371100ENST00000539317GNASchr2057486241-SMAP2chr140878688+0.0078623350.9921376
ENST00000371102ENST00000539317GNASchr2057486241-SMAP2chr140878688+0.0052308140.99476916
ENST00000371095ENST00000539317GNASchr2057486241-SMAP2chr140878688+0.0036466450.9963534
ENST00000371085ENST00000539317GNASchr2057486241-SMAP2chr140878688+0.003545430.9964546
ENST00000354359ENST00000539317GNASchr2057486241-SMAP2chr140878688+0.0026801570.9973199
ENST00000265620ENST00000539317GNASchr2057486241-SMAP2chr140878688+0.0026436460.9973564
ENST00000306090ENST00000539317GNASchr2057486241-SMAP2chr140878688+0.0036868540.99631315

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Fusion Genomic Features for GNAS-SMAP2


check buttonFusionAI prediction of the potential fusion gene breakpoint based on the pre-mature RNA sequence context (+/- 5kb of individual partner genes, total 20kb length sequence). FusionAI is a fusion gene breakpoint classifier based on convolutional neural network by comparing the fusion positive and negative sequence context of ~ 20K fusion gene data. From here, we can have the relative potentency of the 20K genomic sequence how individual sequnce will be likely used as the gene fusion breakpoints.
HgeneHchrHbpHstrandTgeneTchrTbpTstrand1-pp (fusion gene breakpoint)

check buttonDistribution of 44 human genomic features loci across 20kb length fusion breakpoint regions. We integrated a total of 44 different types of human genomic feature loci information across five big categories including virus integration sites, repeats, structural variants, chromatin states, and gene expression regulation. More details are in help page.

check buttonDistribution of 44 human genomic features loci across 20kb length fusion breakpoint regions that are ovelapped with the top 1% feature importance score regions. More details are in help page.

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Fusion Protein Features for GNAS-SMAP2


check button Go to

FGviewer for the breakpoints of chr20:57486241-chr1:40878688

.
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
FGviewer

check buttonMain function of each fusion partner protein. (from UniProt)
HgeneTgene
GNAS

P63092

.
FUNCTION: Guanine nucleotide-binding proteins (G proteins) function as transducers in numerous signaling pathways controlled by G protein-coupled receptors (GPCRs) (PubMed:17110384). Signaling involves the activation of adenylyl cyclases, resulting in increased levels of the signaling molecule cAMP (PubMed:26206488, PubMed:8702665). GNAS functions downstream of several GPCRs, including beta-adrenergic receptors (PubMed:21488135). Stimulates the Ras signaling pathway via RAPGEF2 (PubMed:12391161). {ECO:0000269|PubMed:12391161, ECO:0000269|PubMed:17110384, ECO:0000269|PubMed:21488135, ECO:0000269|PubMed:26206488, ECO:0000269|PubMed:8702665}.FUNCTION: Might normally function as a transcriptional repressor. EWS-fusion-proteins (EFPS) may play a role in the tumorigenic process. They may disturb gene expression by mimicking, or interfering with the normal function of CTD-POLII within the transcription initiation complex. They may also contribute to an aberrant activation of the fusion protein target genes.

check buttonRetention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at

download page


* Minus value of BPloci means that the break pointn is located before the CDS.
- In-frame and retained protein feature among the 13 regional features.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note
HgeneGNASchr20:57486241chr1:40878688ENST00000371100-1313641_6671159.01038.0Coiled coilOntology_term=ECO:0000255
HgeneGNASchr20:57486241chr1:40878688ENST00000371100-1313730_7561159.01038.0Coiled coilOntology_term=ECO:0000255
HgeneGNASchr20:57486241chr1:40878688ENST00000371102-1212641_6671145.01024.0Coiled coilOntology_term=ECO:0000255
HgeneGNASchr20:57486241chr1:40878688ENST00000371102-1212730_7561145.01024.0Coiled coilOntology_term=ECO:0000255
HgeneGNASchr20:57486241chr1:40878688ENST00000313949-131378_142729.666666666666626.333333333333332Compositional biasGlu-rich
HgeneGNASchr20:57486241chr1:40878688ENST00000371075-131378_142730.666666666666636.333333333333336Compositional biasGlu-rich
HgeneGNASchr20:57486241chr1:40878688ENST00000371100-1313358_5221159.01038.0Compositional biasAla-rich
HgeneGNASchr20:57486241chr1:40878688ENST00000371102-1212358_5221145.01024.0Compositional biasAla-rich
HgeneGNASchr20:57486241chr1:40878688ENST00000265620-121239_394501.0380.0DomainG-alpha
HgeneGNASchr20:57486241chr1:40878688ENST00000306090-131339_394500.0381.0DomainG-alpha
HgeneGNASchr20:57486241chr1:40878688ENST00000354359-131339_394517.0396.0DomainG-alpha
HgeneGNASchr20:57486241chr1:40878688ENST00000371085-131339_394516.0395.0DomainG-alpha
HgeneGNASchr20:57486241chr1:40878688ENST00000371095-121239_394502.0381.0DomainG-alpha
HgeneGNASchr20:57486241chr1:40878688ENST00000371100-1313682_10371159.01038.0DomainG-alpha
HgeneGNASchr20:57486241chr1:40878688ENST00000371102-1212682_10371145.01024.0DomainG-alpha
HgeneGNASchr20:57486241chr1:40878688ENST00000265620-1212197_204501.0380.0Nucleotide bindingGTP
HgeneGNASchr20:57486241chr1:40878688ENST00000265620-1212223_227501.0380.0Nucleotide bindingGTP
HgeneGNASchr20:57486241chr1:40878688ENST00000265620-1212292_295501.0380.0Nucleotide bindingGTP
HgeneGNASchr20:57486241chr1:40878688ENST00000265620-121247_55501.0380.0Nucleotide bindingGTP
HgeneGNASchr20:57486241chr1:40878688ENST00000306090-1313197_204500.0381.0Nucleotide bindingGTP
HgeneGNASchr20:57486241chr1:40878688ENST00000306090-1313223_227500.0381.0Nucleotide bindingGTP
HgeneGNASchr20:57486241chr1:40878688ENST00000306090-1313292_295500.0381.0Nucleotide bindingGTP
HgeneGNASchr20:57486241chr1:40878688ENST00000306090-131347_55500.0381.0Nucleotide bindingGTP
HgeneGNASchr20:57486241chr1:40878688ENST00000354359-1313197_204517.0396.0Nucleotide bindingGTP
HgeneGNASchr20:57486241chr1:40878688ENST00000354359-1313223_227517.0396.0Nucleotide bindingGTP
HgeneGNASchr20:57486241chr1:40878688ENST00000354359-1313292_295517.0396.0Nucleotide bindingGTP
HgeneGNASchr20:57486241chr1:40878688ENST00000354359-131347_55517.0396.0Nucleotide bindingGTP
HgeneGNASchr20:57486241chr1:40878688ENST00000371085-1313197_204516.0395.0Nucleotide bindingGTP
HgeneGNASchr20:57486241chr1:40878688ENST00000371085-1313223_227516.0395.0Nucleotide bindingGTP
HgeneGNASchr20:57486241chr1:40878688ENST00000371085-1313292_295516.0395.0Nucleotide bindingGTP
HgeneGNASchr20:57486241chr1:40878688ENST00000371085-131347_55516.0395.0Nucleotide bindingGTP
HgeneGNASchr20:57486241chr1:40878688ENST00000371095-1212197_204502.0381.0Nucleotide bindingGTP
HgeneGNASchr20:57486241chr1:40878688ENST00000371095-1212223_227502.0381.0Nucleotide bindingGTP
HgeneGNASchr20:57486241chr1:40878688ENST00000371095-1212292_295502.0381.0Nucleotide bindingGTP
HgeneGNASchr20:57486241chr1:40878688ENST00000371095-121247_55502.0381.0Nucleotide bindingGTP
HgeneGNASchr20:57486241chr1:40878688ENST00000371100-1313690_6981159.01038.0Nucleotide bindingGTP
HgeneGNASchr20:57486241chr1:40878688ENST00000371100-1313840_8471159.01038.0Nucleotide bindingGTP
HgeneGNASchr20:57486241chr1:40878688ENST00000371100-1313866_8701159.01038.0Nucleotide bindingGTP
HgeneGNASchr20:57486241chr1:40878688ENST00000371100-1313935_9381159.01038.0Nucleotide bindingGTP
HgeneGNASchr20:57486241chr1:40878688ENST00000371102-1212690_6981145.01024.0Nucleotide bindingGTP
HgeneGNASchr20:57486241chr1:40878688ENST00000371102-1212840_8471145.01024.0Nucleotide bindingGTP
HgeneGNASchr20:57486241chr1:40878688ENST00000371102-1212866_8701145.01024.0Nucleotide bindingGTP
HgeneGNASchr20:57486241chr1:40878688ENST00000371102-1212935_9381145.01024.0Nucleotide bindingGTP
HgeneGNASchr20:57486241chr1:40878688ENST00000265620-1212196_204501.0380.0RegionG2 motif
HgeneGNASchr20:57486241chr1:40878688ENST00000265620-1212219_228501.0380.0RegionG3 motif
HgeneGNASchr20:57486241chr1:40878688ENST00000265620-1212288_295501.0380.0RegionG4 motif
HgeneGNASchr20:57486241chr1:40878688ENST00000265620-1212364_369501.0380.0RegionG5 motif
HgeneGNASchr20:57486241chr1:40878688ENST00000265620-121242_55501.0380.0RegionG1 motif
HgeneGNASchr20:57486241chr1:40878688ENST00000306090-1313196_204500.0381.0RegionG2 motif
HgeneGNASchr20:57486241chr1:40878688ENST00000306090-1313219_228500.0381.0RegionG3 motif
HgeneGNASchr20:57486241chr1:40878688ENST00000306090-1313288_295500.0381.0RegionG4 motif
HgeneGNASchr20:57486241chr1:40878688ENST00000306090-1313364_369500.0381.0RegionG5 motif
HgeneGNASchr20:57486241chr1:40878688ENST00000306090-131342_55500.0381.0RegionG1 motif
HgeneGNASchr20:57486241chr1:40878688ENST00000354359-1313196_204517.0396.0RegionG2 motif
HgeneGNASchr20:57486241chr1:40878688ENST00000354359-1313219_228517.0396.0RegionG3 motif
HgeneGNASchr20:57486241chr1:40878688ENST00000354359-1313288_295517.0396.0RegionG4 motif
HgeneGNASchr20:57486241chr1:40878688ENST00000354359-1313364_369517.0396.0RegionG5 motif
HgeneGNASchr20:57486241chr1:40878688ENST00000354359-131342_55517.0396.0RegionG1 motif
HgeneGNASchr20:57486241chr1:40878688ENST00000371085-1313196_204516.0395.0RegionG2 motif
HgeneGNASchr20:57486241chr1:40878688ENST00000371085-1313219_228516.0395.0RegionG3 motif
HgeneGNASchr20:57486241chr1:40878688ENST00000371085-1313288_295516.0395.0RegionG4 motif
HgeneGNASchr20:57486241chr1:40878688ENST00000371085-1313364_369516.0395.0RegionG5 motif
HgeneGNASchr20:57486241chr1:40878688ENST00000371085-131342_55516.0395.0RegionG1 motif
HgeneGNASchr20:57486241chr1:40878688ENST00000371095-1212196_204502.0381.0RegionG2 motif
HgeneGNASchr20:57486241chr1:40878688ENST00000371095-1212219_228502.0381.0RegionG3 motif
HgeneGNASchr20:57486241chr1:40878688ENST00000371095-1212288_295502.0381.0RegionG4 motif
HgeneGNASchr20:57486241chr1:40878688ENST00000371095-1212364_369502.0381.0RegionG5 motif
HgeneGNASchr20:57486241chr1:40878688ENST00000371095-121242_55502.0381.0RegionG1 motif
HgeneGNASchr20:57486241chr1:40878688ENST00000371100-13131007_10121159.01038.0RegionG5 motif
HgeneGNASchr20:57486241chr1:40878688ENST00000371100-1313685_6981159.01038.0RegionG1 motif
HgeneGNASchr20:57486241chr1:40878688ENST00000371100-1313839_8471159.01038.0RegionG2 motif
HgeneGNASchr20:57486241chr1:40878688ENST00000371100-1313862_8711159.01038.0RegionG3 motif
HgeneGNASchr20:57486241chr1:40878688ENST00000371100-1313931_9381159.01038.0RegionG4 motif
HgeneGNASchr20:57486241chr1:40878688ENST00000371102-12121007_10121145.01024.0RegionG5 motif
HgeneGNASchr20:57486241chr1:40878688ENST00000371102-1212685_6981145.01024.0RegionG1 motif
HgeneGNASchr20:57486241chr1:40878688ENST00000371102-1212839_8471145.01024.0RegionG2 motif
HgeneGNASchr20:57486241chr1:40878688ENST00000371102-1212862_8711145.01024.0RegionG3 motif
HgeneGNASchr20:57486241chr1:40878688ENST00000371102-1212931_9381145.01024.0RegionG4 motif
TgeneSMAP2chr20:57486241chr1:40878688ENST00000539317310279_41154.0350.0Compositional biasNote=Met-rich

- In-frame and not-retained protein feature among the 13 regional features.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note
HgeneGNASchr20:57486241chr1:40878688ENST00000306120-11238_4760.0626.0Compositional biasPro-rich
HgeneGNASchr20:57486241chr1:40878688ENST00000371098-1478_142020.333333333333332Compositional biasGlu-rich
TgeneSMAP2chr20:57486241chr1:40878688ENST0000053931731013_13754.0350.0DomainArf-GAP
TgeneSMAP2chr20:57486241chr1:40878688ENST0000053931731028_5154.0350.0Zinc fingerC4-type


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Fusion Gene Sequence for GNAS-SMAP2


check button For in-frame fusion transcripts, we provide the fusion transcript sequences and fusion amino acid sequences. To have fusion amino acid sequence, we ran ORFfinder and chose the longest ORF among the all predicted ones.
>In-frame_ENST00000371100_ENST00000539317_TCGA-H7-7774-01A_GNAS_chr20_57486241_-_SMAP2_chr1_40878688_length(transcript)=5076nt_BP=4029nt
AAAGACTCCAAATCAGTCAGGGAGAGGAGTGGAAGGAGCCAAAACGTCCCTGCGAGGCCTGCGCTCACCAGCCCCCTTACCCAAAGTCCC
GGGCCCCTGGCGCCAGGAGCAGGCTCCTGGAGCCGGAGCCAGCTCGGCACTGGAACCGGCGTCCTCTGGTGGCAGAGAGAGAGCGCTACT
GGCGATTTTCGGACCGAATCGGCACGCTCGTCAGATCCAAGCAGGCGGGACTGGCCTGGAGCAAAAAGAAAGAGAGAGGAGGGCGTAAGG
ATAGACCAAGGAAGAGGGGCTGGGGGGCAGCCTGGGGGCATGAAAAGTGGCCAGGAAGGAGCCAAGACTCCACCAGCAACAATTGAGTTG
CTTCAGCCTCAGTCTAGGGTTCCTTCCAGGCCTTGAACCCCCCAACCTCACAAGGGTTGGAAAGTGAGGCCGGTGAACTTTCCAGCTGGT
ACTTTGATTTTAAAATAATAATAATAATTTTTTCACCCTAGTTCGGTTGGGTGCTCCATCTTACGGAGCCCCAAACTTATTTTGAGAGGC
CGCCACCGTGTTATGGGCGTGCGCAACTGCCTCTACGGCAATAATATGTCAGGACAACGCGATATCCCCCCTGAAATCGGGGAACAGCCC
GAGCAACCACCTTTGGAGGCCCCAGGGGCAGCTGCCCCCGGTGCTGGGCCTAGCCCAGCCGAAGAGATGGAGACCGAACCGCCTCACAAC
GAGCCCATCCCCGTCGAGAATGATGGCGAGGCCTGTGGACCCCCAGAGGTCTCCAGACCCAACTTTCAGGTCCTCAACCCGGCATTCAGG
GAAGCTGGAGCCCATGGAAGCTACAGCCCACCTCCTGAGGAAGCAATGCCCTTCGAGGCTGAACAGCCCAGCTTGGGAGGCTTCTGGCCT
ACACTGGAGCAGCCTGGATTCCCCAGTGGGGTCCATGCAGGCCTTGAGGCCTTCGGCCCAGCACTCATGGAGCCCGGAGCCTTCAGTGGT
GCCAGACCAGGCCTGGGAGGATACAGCCCTCCACCAGAAGAAGCTATGCCCTTTGAGTTTGACCAGCCTGCCCAGAGAGGCTGCAGTCAA
CTTCTCTTACAGGTCCCAGACCTTGCTCCAGGAGGCCCAGGTGCTGCAGGGGTCCCCGGAGCTCCTCCCGAGGAGCCCCAAGCCCTCAGG
CCTGCAAAGGCTGGCTCCAGAGGAGGCTACAGCCCTCCCCCTGAGGAGACTATGCCATTTGAGCTTGATGGAGAAGGATTTGGGGACGAC
AGCCCACCCCCGGGGCTTTCCCGAGTTATCGCACAAGTCGACGGCAGCAGCCAGTTCGCGGCAGTCGCGGCCTCGAGTGCGGTCCGCCTC
ACTCCCGCCGCGAACGCGCCTCCCCTCTGGGTCCCAGGCGCCATCGGCAGCCCATCCCAAGAGGCTGTCAGACCTCCTTCTAACTTCACG
GGCAGCAGCCCCTGGATGGAGATCTCCGGACCCCCGTTCGAGATTGGCAGCGCCCCCGCTGGGGTCGACGACACTCCCGTCAACATGGAC
AGCCCCCCAATCGCGCTTGACGGCCCGCCCATCAAGGTCTCCGGAGCCCCAGATAAGAGAGAGCGAGCAGAGAGACCCCCAGTTGAGGAG
GAAGCAGCAGAGATGGAAGGAGCCGCTGATGCCGCGGAGGGAGGAAAAGTACCCTCTCCGGGGTACGGATCCCCTGCCGCCGGGGCAGCC
TCAGCGGATACCGCTGCCAGGGCAGCCCCTGCAGCCCCAGCCGATCCTGACTCCGGGGCAACCCCAGAAGATCCCGACTCCGGGACAGCA
CCAGCCGATCCTGACTCCGGGGCATTCGCAGCCGATCCCGACTCCGGGGCAGCCCCTGCCGCCCCAGCCGATCCCGACTCCGGGGCGGCC
CCTGACGCCCCAGCCGATCCCGACTCCGGGGCGGCCCCTGACGCCCCAGCCGATCCAGATGCCGGGGCGGCCCCTGAGGCTCCCGCCGCC
CCTGCGGCTGCTGAGACCCGGGCAGCCCATGTCGCCCCAGCTGCGCCAGACGCAGGGGCTCCCACTGCCCCAGCCGCTTCTGCCACCCGG
GCAGCCCAAGTCCGCCGGGCGGCCTCTGCAGCCCCTGCCTCCGGGGCCAGACGCAAGATCCATCTCAGACCCCCCAGCCCCGAGATCCAG
GCTGCCGATCCGCCTACTCCGCGGCCTACTCGCGCGTCTGCCTGGCGGGGCAAGTCCGAGAGCAGCCGCGGCCGCCGCGTGTACTACGAT
GAAGGGGTGGCCAGCAGCGACGATGACTCCAGCGGAGACGAGTCCGACGATGGGACCTCCGGATGCCTCCGCTGGTTTCAGCATCGGCGA
AATCGCCGCCGCCGAAAGCCCCAGCGCAACTTACTCCGCAACTTTCTCGTGCAAGCCTTCGGGGGCTGCTTCGGTCGATCTGAGAGTCCC
CAGCCCAAAGCCTCGCGCTCTCTCAAGGTCAAGAAGGTACCCCTGGCGGAGAAGCGCAGACAGATGCGCAAAGAAGCCCTGGAGAAGCGG
GCCCAGAAGCGCGCAGAGAAGAAACGCAGTAAGCTCATCGACAAACAACTCCAGGACGAAAAGATGGGCTACATGTGTACGCACCGCCTG
CTGCTTCTAGGTGCTGGAGAATCTGGTAAAAGCACCATTGTGAAGCAGATGAGGATCCTGCATGTTAATGGGTTTAATGGAGAGGGCGGC
GAAGAGGACCCGCAGGCTGCAAGGAGCAACAGCGATGGTGAGAAGGCAACCAAAGTGCAGGACATCAAAAACAACCTGAAAGAGGCGATT
GAAACCATTGTGGCCGCCATGAGCAACCTGGTGCCCCCCGTGGAGCTGGCCAACCCCGAGAACCAGTTCAGAGTGGACTACATCCTGAGT
GTGATGAACGTGCCTGACTTTGACTTCCCTCCCGAATTCTATGAGCATGCCAAGGCTCTGTGGGAGGATGAAGGAGTGCGTGCCTGCTAC
GAACGCTCCAACGAGTACCAGCTGATTGACTGTGCCCAGTACTTCCTGGACAAGATCGACGTGATCAAGCAGGCTGACTATGTGCCGAGC
GATCAGGACCTGCTTCGCTGCCGTGTCCTGACTTCTGGAATCTTTGAGACCAAGTTCCAGGTGGACAAAGTCAACTTCCACATGTTTGAC
GTGGGTGGCCAGCGCGATGAACGCCGCAAGTGGATCCAGTGCTTCAACGATGTGACTGCCATCATCTTCGTGGTGGCCAGCAGCAGCTAC
AACATGGTCATCCGGGAGGACAACCAGACCAACCGCCTGCAGGAGGCTCTGAACCTCTTCAAGAGCATCTGGAACAACAGATGGCTGCGC
ACCATCTCTGTGATCCTGTTCCTCAACAAGCAAGATCTGCTCGCTGAGAAAGTCCTTGCTGGGAAATCGAAGATTGAGGACTACTTTCCA
GAATTTGCTCGCTACACTACTCCTGAGGATGCTACTCCCGAGCCCGGAGAGGACCCACGCGTGACCCGGGCCAAGTACTTCATTCGAGAT
GAGTTTCTGAGGATCAGCACTGCCAGTGGAGATGGGCGTCACTACTGCTACCCTCATTTCACCTGCGCTGTGGACACTGAGAACATCCGC
CGTGTGTTCAACGACTGCCGTGACATCATTCAGCGCATGCACCTTCGTCAGTACGAGCTGCTCTAAGAAGGGAACCCCCAAATTTAATTA
AAGCCTTAAGCACAATTAATTAAAAGTGAAACGTAATTGTACAAGCAGTTAATCACCCACCATAGGGCATGATTAACAAAGCAACCTTTC
CCTTCCCCCGAGTGATTTTGCGAAACCCCCTTTTCCCTTCAGCTTGCTTAGATGTTCCAAATTTAGAAAGCTTAAGGCGGCCTACAGAAA
AAGGAAAAAAGGCCACAAAAGTTCCCTCTCACTTTCAGTAAAAATAAATAAAACAGCAGCAGCAAACAAATAAAATGAAATAAAAGAAAC
AAATGAAATAAATATTGTGTTGTGCAGCATTAAAAAAAATCAAAATAAAAATTAAATGTGAGCAAAGAAAAAGAAAAAGATGACAAGTGG
AAAAGAGGGAGCGAACCAGTTCCAGAAAAAAAATTGGAACCTGTTGTTTTTGAGAAGGTGAAAATGCCACAGAAAAAAGAAGACCCACAG
CTACCTCGGAAAAGCTCCCCGAAATCCACAGCGCCTGTCATGGATTTGTTGGGCCTTGATGCTCCTGTGGCCTGCTCCATTGCAAATAGT
AAGACCAGCAATACCCTAGAGAAGGATTTAGATCTGTTGGCCTCTGTTCCATCCCCTTCTTCTTCCGGTTCCAGAAAGGTTGTAGGTTCC
ATGCCAACTGCAGGGAGTGCCGGCTCTGTTCCTGAAAATCTGAACCTGTTTCCGGAGCCAGGGAGCAAATCAGAAGAAATAGGCAAGAAA
CAGCTCTCTAAAGACTCCATTCTTTCACTGTATGGATCCCAGACGCCTCAAATGCCTACTCAAGCAATGTTCATGGCTCCCGCTCAGATG
GCATATCCCACAGCCTACCCCAGCTTCCCCGGGGTTACACCTCCTAACAGCATAATGGGGAGCATGATGCCTCCACCAGTAGGCATGGTT
GCTCAGCCAGGAGCTTCTGGGATGGTTGCCCCCATGGCCATGCCTGCAGGCTATATGGGTGGCATGCAGGCATCAATGATGGGTGTGCCG
AATGGAATGATGACCACCCAGCAGGCTGGCTACATGGCAGGCATGGCAGCTATGCCCCAGACTGTGTATGGGGTCCAGCCAGCTCAGCAG
CTGCAATGGAACCTTACTCAGATGACCCAGCAGATGGCTGGGATGAACTTCTATGGAGCCAATGGCATGATGAACTATGGACAGTCAATG
AGTGGCGGAAATGGACAGGCAGCAAATCAGACTCTCAGTCCTCAGATGTGGAAATAAAAACAAAACACCTGTATGGCTGCCATTCTCTTC
AGCCCTCGCTCTCCCCTTTCCACAGCCTCCACCCCTGACCCCCATCCTCTTTTCCTACCTCTCTGTTTGGTTTAGAAATTGCTCAATAAG

>In-frame_ENST00000371100_ENST00000539317_TCGA-H7-7774-01A_GNAS_chr20_57486241_-_SMAP2_chr1_40878688_length(amino acids)=1044AA_start in transcript=531_stop in transcript=3665
MRGRHRVMGVRNCLYGNNMSGQRDIPPEIGEQPEQPPLEAPGAAAPGAGPSPAEEMETEPPHNEPIPVENDGEACGPPEVSRPNFQVLNP
AFREAGAHGSYSPPPEEAMPFEAEQPSLGGFWPTLEQPGFPSGVHAGLEAFGPALMEPGAFSGARPGLGGYSPPPEEAMPFEFDQPAQRG
CSQLLLQVPDLAPGGPGAAGVPGAPPEEPQALRPAKAGSRGGYSPPPEETMPFELDGEGFGDDSPPPGLSRVIAQVDGSSQFAAVAASSA
VRLTPAANAPPLWVPGAIGSPSQEAVRPPSNFTGSSPWMEISGPPFEIGSAPAGVDDTPVNMDSPPIALDGPPIKVSGAPDKRERAERPP
VEEEAAEMEGAADAAEGGKVPSPGYGSPAAGAASADTAARAAPAAPADPDSGATPEDPDSGTAPADPDSGAFAADPDSGAAPAAPADPDS
GAAPDAPADPDSGAAPDAPADPDAGAAPEAPAAPAAAETRAAHVAPAAPDAGAPTAPAASATRAAQVRRAASAAPASGARRKIHLRPPSP
EIQAADPPTPRPTRASAWRGKSESSRGRRVYYDEGVASSDDDSSGDESDDGTSGCLRWFQHRRNRRRRKPQRNLLRNFLVQAFGGCFGRS
ESPQPKASRSLKVKKVPLAEKRRQMRKEALEKRAQKRAEKKRSKLIDKQLQDEKMGYMCTHRLLLLGAGESGKSTIVKQMRILHVNGFNG
EGGEEDPQAARSNSDGEKATKVQDIKNNLKEAIETIVAAMSNLVPPVELANPENQFRVDYILSVMNVPDFDFPPEFYEHAKALWEDEGVR
ACYERSNEYQLIDCAQYFLDKIDVIKQADYVPSDQDLLRCRVLTSGIFETKFQVDKVNFHMFDVGGQRDERRKWIQCFNDVTAIIFVVAS
SSYNMVIREDNQTNRLQEALNLFKSIWNNRWLRTISVILFLNKQDLLAEKVLAGKSKIEDYFPEFARYTTPEDATPEPGEDPRVTRAKYF

--------------------------------------------------------------
>In-frame_ENST00000371102_ENST00000539317_TCGA-H7-7774-01A_GNAS_chr20_57486241_-_SMAP2_chr1_40878688_length(transcript)=4485nt_BP=3438nt
GTTATGGGCGTGCGCAACTGCCTCTACGGCAATAATATGTCAGGACAACGCGATATCCCCCCTGAAATCGGGGAACAGCCCGAGCAACCA
CCTTTGGAGGCCCCAGGGGCAGCTGCCCCCGGTGCTGGGCCTAGCCCAGCCGAAGAGATGGAGACCGAACCGCCTCACAACGAGCCCATC
CCCGTCGAGAATGATGGCGAGGCCTGTGGACCCCCAGAGGTCTCCAGACCCAACTTTCAGGTCCTCAACCCGGCATTCAGGGAAGCTGGA
GCCCATGGAAGCTACAGCCCACCTCCTGAGGAAGCAATGCCCTTCGAGGCTGAACAGCCCAGCTTGGGAGGCTTCTGGCCTACACTGGAG
CAGCCTGGATTCCCCAGTGGGGTCCATGCAGGCCTTGAGGCCTTCGGCCCAGCACTCATGGAGCCCGGAGCCTTCAGTGGTGCCAGACCA
GGCCTGGGAGGATACAGCCCTCCACCAGAAGAAGCTATGCCCTTTGAGTTTGACCAGCCTGCCCAGAGAGGCTGCAGTCAACTTCTCTTA
CAGGTCCCAGACCTTGCTCCAGGAGGCCCAGGTGCTGCAGGGGTCCCCGGAGCTCCTCCCGAGGAGCCCCAAGCCCTCAGGCCTGCAAAG
GCTGGCTCCAGAGGAGGCTACAGCCCTCCCCCTGAGGAGACTATGCCATTTGAGCTTGATGGAGAAGGATTTGGGGACGACAGCCCACCC
CCGGGGCTTTCCCGAGTTATCGCACAAGTCGACGGCAGCAGCCAGTTCGCGGCAGTCGCGGCCTCGAGTGCGGTCCGCCTCACTCCCGCC
GCGAACGCGCCTCCCCTCTGGGTCCCAGGCGCCATCGGCAGCCCATCCCAAGAGGCTGTCAGACCTCCTTCTAACTTCACGGGCAGCAGC
CCCTGGATGGAGATCTCCGGACCCCCGTTCGAGATTGGCAGCGCCCCCGCTGGGGTCGACGACACTCCCGTCAACATGGACAGCCCCCCA
ATCGCGCTTGACGGCCCGCCCATCAAGGTCTCCGGAGCCCCAGATAAGAGAGAGCGAGCAGAGAGACCCCCAGTTGAGGAGGAAGCAGCA
GAGATGGAAGGAGCCGCTGATGCCGCGGAGGGAGGAAAAGTACCCTCTCCGGGGTACGGATCCCCTGCCGCCGGGGCAGCCTCAGCGGAT
ACCGCTGCCAGGGCAGCCCCTGCAGCCCCAGCCGATCCTGACTCCGGGGCAACCCCAGAAGATCCCGACTCCGGGACAGCACCAGCCGAT
CCTGACTCCGGGGCATTCGCAGCCGATCCCGACTCCGGGGCAGCCCCTGCCGCCCCAGCCGATCCCGACTCCGGGGCGGCCCCTGACGCC
CCAGCCGATCCCGACTCCGGGGCGGCCCCTGACGCCCCAGCCGATCCAGATGCCGGGGCGGCCCCTGAGGCTCCCGCCGCCCCTGCGGCT
GCTGAGACCCGGGCAGCCCATGTCGCCCCAGCTGCGCCAGACGCAGGGGCTCCCACTGCCCCAGCCGCTTCTGCCACCCGGGCAGCCCAA
GTCCGCCGGGCGGCCTCTGCAGCCCCTGCCTCCGGGGCCAGACGCAAGATCCATCTCAGACCCCCCAGCCCCGAGATCCAGGCTGCCGAT
CCGCCTACTCCGCGGCCTACTCGCGCGTCTGCCTGGCGGGGCAAGTCCGAGAGCAGCCGCGGCCGCCGCGTGTACTACGATGAAGGGGTG
GCCAGCAGCGACGATGACTCCAGCGGAGACGAGTCCGACGATGGGACCTCCGGATGCCTCCGCTGGTTTCAGCATCGGCGAAATCGCCGC
CGCCGAAAGCCCCAGCGCAACTTACTCCGCAACTTTCTCGTGCAAGCCTTCGGGGGCTGCTTCGGTCGATCTGAGAGTCCCCAGCCCAAA
GCCTCGCGCTCTCTCAAGGTCAAGAAGGTACCCCTGGCGGAGAAGCGCAGACAGATGCGCAAAGAAGCCCTGGAGAAGCGGGCCCAGAAG
CGCGCAGAGAAGAAACGCAGTAAGCTCATCGACAAACAACTCCAGGACGAAAAGATGGGCTACATGTGTACGCACCGCCTGCTGCTTCTA
GGTGCTGGAGAATCTGGTAAAAGCACCATTGTGAAGCAGATGAGGATCCTGCATGTTAATGGGTTTAATGGAGACAGTGAGAAGGCAACC
AAAGTGCAGGACATCAAAAACAACCTGAAAGAGGCGATTGAAACCATTGTGGCCGCCATGAGCAACCTGGTGCCCCCCGTGGAGCTGGCC
AACCCCGAGAACCAGTTCAGAGTGGACTACATCCTGAGTGTGATGAACGTGCCTGACTTTGACTTCCCTCCCGAATTCTATGAGCATGCC
AAGGCTCTGTGGGAGGATGAAGGAGTGCGTGCCTGCTACGAACGCTCCAACGAGTACCAGCTGATTGACTGTGCCCAGTACTTCCTGGAC
AAGATCGACGTGATCAAGCAGGCTGACTATGTGCCGAGCGATCAGGACCTGCTTCGCTGCCGTGTCCTGACTTCTGGAATCTTTGAGACC
AAGTTCCAGGTGGACAAAGTCAACTTCCACATGTTTGACGTGGGTGGCCAGCGCGATGAACGCCGCAAGTGGATCCAGTGCTTCAACGAT
GTGACTGCCATCATCTTCGTGGTGGCCAGCAGCAGCTACAACATGGTCATCCGGGAGGACAACCAGACCAACCGCCTGCAGGAGGCTCTG
AACCTCTTCAAGAGCATCTGGAACAACAGATGGCTGCGCACCATCTCTGTGATCCTGTTCCTCAACAAGCAAGATCTGCTCGCTGAGAAA
GTCCTTGCTGGGAAATCGAAGATTGAGGACTACTTTCCAGAATTTGCTCGCTACACTACTCCTGAGGATGCTACTCCCGAGCCCGGAGAG
GACCCACGCGTGACCCGGGCCAAGTACTTCATTCGAGATGAGTTTCTGAGGATCAGCACTGCCAGTGGAGATGGGCGTCACTACTGCTAC
CCTCATTTCACCTGCGCTGTGGACACTGAGAACATCCGCCGTGTGTTCAACGACTGCCGTGACATCATTCAGCGCATGCACCTTCGTCAG
TACGAGCTGCTCTAAGAAGGGAACCCCCAAATTTAATTAAAGCCTTAAGCACAATTAATTAAAAGTGAAACGTAATTGTACAAGCAGTTA
ATCACCCACCATAGGGCATGATTAACAAAGCAACCTTTCCCTTCCCCCGAGTGATTTTGCGAAACCCCCTTTTCCCTTCAGCTTGCTTAG
ATGTTCCAAATTTAGAAAGCTTAAGGCGGCCTACAGAAAAAGGAAAAAAGGCCACAAAAGTTCCCTCTCACTTTCAGTAAAAATAAATAA
AACAGCAGCAGCAAACAAATAAAATGAAATAAAAGAAACAAATGAAATAAATATTGTGTTGTGCAGCATTAAAAAAAATCAAAATAAAAA
TTAAATGTGAGCAAAGAAAAAGAAAAAGATGACAAGTGGAAAAGAGGGAGCGAACCAGTTCCAGAAAAAAAATTGGAACCTGTTGTTTTT
GAGAAGGTGAAAATGCCACAGAAAAAAGAAGACCCACAGCTACCTCGGAAAAGCTCCCCGAAATCCACAGCGCCTGTCATGGATTTGTTG
GGCCTTGATGCTCCTGTGGCCTGCTCCATTGCAAATAGTAAGACCAGCAATACCCTAGAGAAGGATTTAGATCTGTTGGCCTCTGTTCCA
TCCCCTTCTTCTTCCGGTTCCAGAAAGGTTGTAGGTTCCATGCCAACTGCAGGGAGTGCCGGCTCTGTTCCTGAAAATCTGAACCTGTTT
CCGGAGCCAGGGAGCAAATCAGAAGAAATAGGCAAGAAACAGCTCTCTAAAGACTCCATTCTTTCACTGTATGGATCCCAGACGCCTCAA
ATGCCTACTCAAGCAATGTTCATGGCTCCCGCTCAGATGGCATATCCCACAGCCTACCCCAGCTTCCCCGGGGTTACACCTCCTAACAGC
ATAATGGGGAGCATGATGCCTCCACCAGTAGGCATGGTTGCTCAGCCAGGAGCTTCTGGGATGGTTGCCCCCATGGCCATGCCTGCAGGC
TATATGGGTGGCATGCAGGCATCAATGATGGGTGTGCCGAATGGAATGATGACCACCCAGCAGGCTGGCTACATGGCAGGCATGGCAGCT
ATGCCCCAGACTGTGTATGGGGTCCAGCCAGCTCAGCAGCTGCAATGGAACCTTACTCAGATGACCCAGCAGATGGCTGGGATGAACTTC
TATGGAGCCAATGGCATGATGAACTATGGACAGTCAATGAGTGGCGGAAATGGACAGGCAGCAAATCAGACTCTCAGTCCTCAGATGTGG
AAATAAAAACAAAACACCTGTATGGCTGCCATTCTCTTCAGCCCTCGCTCTCCCCTTTCCACAGCCTCCACCCCTGACCCCCATCCTCTT

>In-frame_ENST00000371102_ENST00000539317_TCGA-H7-7774-01A_GNAS_chr20_57486241_-_SMAP2_chr1_40878688_length(amino acids)=1023AA_start in transcript=3_stop in transcript=3074
MGVRNCLYGNNMSGQRDIPPEIGEQPEQPPLEAPGAAAPGAGPSPAEEMETEPPHNEPIPVENDGEACGPPEVSRPNFQVLNPAFREAGA
HGSYSPPPEEAMPFEAEQPSLGGFWPTLEQPGFPSGVHAGLEAFGPALMEPGAFSGARPGLGGYSPPPEEAMPFEFDQPAQRGCSQLLLQ
VPDLAPGGPGAAGVPGAPPEEPQALRPAKAGSRGGYSPPPEETMPFELDGEGFGDDSPPPGLSRVIAQVDGSSQFAAVAASSAVRLTPAA
NAPPLWVPGAIGSPSQEAVRPPSNFTGSSPWMEISGPPFEIGSAPAGVDDTPVNMDSPPIALDGPPIKVSGAPDKRERAERPPVEEEAAE
MEGAADAAEGGKVPSPGYGSPAAGAASADTAARAAPAAPADPDSGATPEDPDSGTAPADPDSGAFAADPDSGAAPAAPADPDSGAAPDAP
ADPDSGAAPDAPADPDAGAAPEAPAAPAAAETRAAHVAPAAPDAGAPTAPAASATRAAQVRRAASAAPASGARRKIHLRPPSPEIQAADP
PTPRPTRASAWRGKSESSRGRRVYYDEGVASSDDDSSGDESDDGTSGCLRWFQHRRNRRRRKPQRNLLRNFLVQAFGGCFGRSESPQPKA
SRSLKVKKVPLAEKRRQMRKEALEKRAQKRAEKKRSKLIDKQLQDEKMGYMCTHRLLLLGAGESGKSTIVKQMRILHVNGFNGDSEKATK
VQDIKNNLKEAIETIVAAMSNLVPPVELANPENQFRVDYILSVMNVPDFDFPPEFYEHAKALWEDEGVRACYERSNEYQLIDCAQYFLDK
IDVIKQADYVPSDQDLLRCRVLTSGIFETKFQVDKVNFHMFDVGGQRDERRKWIQCFNDVTAIIFVVASSSYNMVIREDNQTNRLQEALN
LFKSIWNNRWLRTISVILFLNKQDLLAEKVLAGKSKIEDYFPEFARYTTPEDATPEPGEDPRVTRAKYFIRDEFLRISTASGDGRHYCYP

--------------------------------------------------------------
>In-frame_ENST00000371095_ENST00000539317_TCGA-H7-7774-01A_GNAS_chr20_57486241_-_SMAP2_chr1_40878688_length(transcript)=2978nt_BP=1931nt
CTCGCCGCCGCCGCCTCCTCCTCCCCCCGCCTCCCCCCGCCCGCCGCGGCGCTTTTGGCTCGGGGCGGCGGCGGGGGCCCGGCCGAGGCA
ATAAGAGCGGCGGCGGCGGCAGCGGCGGCAGCAGCTCCCGCAGCTCCTGCTCTGGTCCGCCTCGGCCCGGCGGCGGCCATCAGCCCCCTC
GGCCTCGGCTCGAGGGGCGGGGAGCTGCGCGCGCCCCTCGGTCCGACCGACACCCTCCCCTTCCCGCCCGTCCGCGCGCCCCGCGGCCCG
CGGCCCGCAGTCCGCCCCGCGCGCTCCTTGCCGAGGAGCCGAGCCCGCGCCCGGCCCGCCCGCCCGGCGCTGCCCCGGCCCTCCCGGCCC
GCGTGAGGCCGCCCGCGCCCGCCGCCGCCGCAGCCCGGCCGCGCCCCGCCGCCGCCGCCGCCGCCATGGGCTGCCTCGGGAACAGTAAGA
CCGAGGACCAGCGCAACGAGGAGAAGGCGCAGCGTGAGGCCAACAAAAAGATCGAGAAGCAGCTGCAGAAGGACAAGCAGGTCTACCGGG
CCACGCACCGCCTGCTGCTGCTGGGTGCTGGAGAATCTGGTAAAAGCACCATTGTGAAGCAGATGAGGATCCTGCATGTTAATGGGTTTA
ATGGAGACAGTGAGAAGGCAACCAAAGTGCAGGACATCAAAAACAACCTGAAAGAGGCGATTGAAACCATTGTGGCCGCCATGAGCAACC
TGGTGCCCCCCGTGGAGCTGGCCAACCCCGAGAACCAGTTCAGAGTGGACTACATCCTGAGTGTGATGAACGTGCCTGACTTTGACTTCC
CTCCCGAATTCTATGAGCATGCCAAGGCTCTGTGGGAGGATGAAGGAGTGCGTGCCTGCTACGAACGCTCCAACGAGTACCAGCTGATTG
ACTGTGCCCAGTACTTCCTGGACAAGATCGACGTGATCAAGCAGGCTGACTATGTGCCGAGCGATCAGGACCTGCTTCGCTGCCGTGTCC
TGACTTCTGGAATCTTTGAGACCAAGTTCCAGGTGGACAAAGTCAACTTCCACATGTTTGACGTGGGTGGCCAGCGCGATGAACGCCGCA
AGTGGATCCAGTGCTTCAACGATGTGACTGCCATCATCTTCGTGGTGGCCAGCAGCAGCTACAACATGGTCATCCGGGAGGACAACCAGA
CCAACCGCCTGCAGGAGGCTCTGAACCTCTTCAAGAGCATCTGGAACAACAGATGGCTGCGCACCATCTCTGTGATCCTGTTCCTCAACA
AGCAAGATCTGCTCGCTGAGAAAGTCCTTGCTGGGAAATCGAAGATTGAGGACTACTTTCCAGAATTTGCTCGCTACACTACTCCTGAGG
ATGCTACTCCCGAGCCCGGAGAGGACCCACGCGTGACCCGGGCCAAGTACTTCATTCGAGATGAGTTTCTGAGGATCAGCACTGCCAGTG
GAGATGGGCGTCACTACTGCTACCCTCATTTCACCTGCGCTGTGGACACTGAGAACATCCGCCGTGTGTTCAACGACTGCCGTGACATCA
TTCAGCGCATGCACCTTCGTCAGTACGAGCTGCTCTAAGAAGGGAACCCCCAAATTTAATTAAAGCCTTAAGCACAATTAATTAAAAGTG
AAACGTAATTGTACAAGCAGTTAATCACCCACCATAGGGCATGATTAACAAAGCAACCTTTCCCTTCCCCCGAGTGATTTTGCGAAACCC
CCTTTTCCCTTCAGCTTGCTTAGATGTTCCAAATTTAGAAAGCTTAAGGCGGCCTACAGAAAAAGGAAAAAAGGCCACAAAAGTTCCCTC
TCACTTTCAGTAAAAATAAATAAAACAGCAGCAGCAAACAAATAAAATGAAATAAAAGAAACAAATGAAATAAATATTGTGTTGTGCAGC
ATTAAAAAAAATCAAAATAAAAATTAAATGTGAGCAAAGAAAAAGAAAAAGATGACAAGTGGAAAAGAGGGAGCGAACCAGTTCCAGAAA
AAAAATTGGAACCTGTTGTTTTTGAGAAGGTGAAAATGCCACAGAAAAAAGAAGACCCACAGCTACCTCGGAAAAGCTCCCCGAAATCCA
CAGCGCCTGTCATGGATTTGTTGGGCCTTGATGCTCCTGTGGCCTGCTCCATTGCAAATAGTAAGACCAGCAATACCCTAGAGAAGGATT
TAGATCTGTTGGCCTCTGTTCCATCCCCTTCTTCTTCCGGTTCCAGAAAGGTTGTAGGTTCCATGCCAACTGCAGGGAGTGCCGGCTCTG
TTCCTGAAAATCTGAACCTGTTTCCGGAGCCAGGGAGCAAATCAGAAGAAATAGGCAAGAAACAGCTCTCTAAAGACTCCATTCTTTCAC
TGTATGGATCCCAGACGCCTCAAATGCCTACTCAAGCAATGTTCATGGCTCCCGCTCAGATGGCATATCCCACAGCCTACCCCAGCTTCC
CCGGGGTTACACCTCCTAACAGCATAATGGGGAGCATGATGCCTCCACCAGTAGGCATGGTTGCTCAGCCAGGAGCTTCTGGGATGGTTG
CCCCCATGGCCATGCCTGCAGGCTATATGGGTGGCATGCAGGCATCAATGATGGGTGTGCCGAATGGAATGATGACCACCCAGCAGGCTG
GCTACATGGCAGGCATGGCAGCTATGCCCCAGACTGTGTATGGGGTCCAGCCAGCTCAGCAGCTGCAATGGAACCTTACTCAGATGACCC
AGCAGATGGCTGGGATGAACTTCTATGGAGCCAATGGCATGATGAACTATGGACAGTCAATGAGTGGCGGAAATGGACAGGCAGCAAATC
AGACTCTCAGTCCTCAGATGTGGAAATAAAAACAAAACACCTGTATGGCTGCCATTCTCTTCAGCCCTCGCTCTCCCCTTTCCACAGCCT
CCACCCCTGACCCCCATCCTCTTTTCCTACCTCTCTGTTTGGTTTAGAAATTGCTCAATAAGTCATTTGGGGTTTGGCATCCTGCCCAGC

>In-frame_ENST00000371095_ENST00000539317_TCGA-H7-7774-01A_GNAS_chr20_57486241_-_SMAP2_chr1_40878688_length(amino acids)=527AA_start in transcript=1582_stop in transcript=2
MGVPFLEQLVLTKVHALNDVTAVVEHTADVLSVHSAGEMRVAVVTPISTGSADPQKLISNEVLGPGHAWVLSGLGSSILRSSVASKFWKV
VLNLRFPSKDFLSEQILLVEEQDHRDGAQPSVVPDALEEVQSLLQAVGLVVLPDDHVVAAAGHHEDDGSHIVEALDPLAAFIALATHVKH
VEVDFVHLELGLKDSRSQDTAAKQVLIARHIVSLLDHVDLVQEVLGTVNQLVLVGAFVAGTHSFILPQSLGMLIEFGREVKVRHVHHTQD
VVHSELVLGVGQLHGGHQVAHGGHNGFNRLFQVVFDVLHFGCLLTVSIKPINMQDPHLLHNGAFTRFSSTQQQQAVRGPVDLLVLLQLLL
DLFVGLTLRLLLVALVLGLTVPEAAHGGGGGGGARPGCGGGGRGRPHAGREGRGSAGRAGRARARLLGKERAGRTAGRGPRGARTGGKGR

--------------------------------------------------------------
>In-frame_ENST00000371085_ENST00000539317_TCGA-H7-7774-01A_GNAS_chr20_57486241_-_SMAP2_chr1_40878688_length(transcript)=3019nt_BP=1972nt
TCGCCGCCGCCGCCTCCTCCTCCCCCCGCCTCCCCCCGCCCGCCGCGGCGCTTTTGGCTCGGGGCGGCGGCGGGGGCCCGGCCGAGGCAA
TAAGAGCGGCGGCGGCGGCAGCGGCGGCAGCAGCTCCCGCAGCTCCTGCTCTGGTCCGCCTCGGCCCGGCGGCGGCCATCAGCCCCCTCG
GCCTCGGCTCGAGGGGCGGGGAGCTGCGCGCGCCCCTCGGTCCGACCGACACCCTCCCCTTCCCGCCCGTCCGCGCGCCCCGCGGCCCGC
GGCCCGCAGTCCGCCCCGCGCGCTCCTTGCCGAGGAGCCGAGCCCGCGCCCGGCCCGCCCGCCCGGCGCTGCCCCGGCCCTCCCGGCCCG
CGTGAGGCCGCCCGCGCCCGCCGCCGCCGCAGCCCGGCCGCGCCCCGCCGCCGCCGCCGCCGCCATGGGCTGCCTCGGGAACAGTAAGAC
CGAGGACCAGCGCAACGAGGAGAAGGCGCAGCGTGAGGCCAACAAAAAGATCGAGAAGCAGCTGCAGAAGGACAAGCAGGTCTACCGGGC
CACGCACCGCCTGCTGCTGCTGGGTGCTGGAGAATCTGGTAAAAGCACCATTGTGAAGCAGATGAGGATCCTGCATGTTAATGGGTTTAA
TGGAGAGGGCGGCGAAGAGGACCCGCAGGCTGCAAGGAGCAACAGCGATGGTGAGAAGGCAACCAAAGTGCAGGACATCAAAAACAACCT
GAAAGAGGCGATTGAAACCATTGTGGCCGCCATGAGCAACCTGGTGCCCCCCGTGGAGCTGGCCAACCCCGAGAACCAGTTCAGAGTGGA
CTACATCCTGAGTGTGATGAACGTGCCTGACTTTGACTTCCCTCCCGAATTCTATGAGCATGCCAAGGCTCTGTGGGAGGATGAAGGAGT
GCGTGCCTGCTACGAACGCTCCAACGAGTACCAGCTGATTGACTGTGCCCAGTACTTCCTGGACAAGATCGACGTGATCAAGCAGGCTGA
CTATGTGCCGAGCGATCAGGACCTGCTTCGCTGCCGTGTCCTGACTTCTGGAATCTTTGAGACCAAGTTCCAGGTGGACAAAGTCAACTT
CCACATGTTTGACGTGGGTGGCCAGCGCGATGAACGCCGCAAGTGGATCCAGTGCTTCAACGATGTGACTGCCATCATCTTCGTGGTGGC
CAGCAGCAGCTACAACATGGTCATCCGGGAGGACAACCAGACCAACCGCCTGCAGGAGGCTCTGAACCTCTTCAAGAGCATCTGGAACAA
CAGATGGCTGCGCACCATCTCTGTGATCCTGTTCCTCAACAAGCAAGATCTGCTCGCTGAGAAAGTCCTTGCTGGGAAATCGAAGATTGA
GGACTACTTTCCAGAATTTGCTCGCTACACTACTCCTGAGGATGCTACTCCCGAGCCCGGAGAGGACCCACGCGTGACCCGGGCCAAGTA
CTTCATTCGAGATGAGTTTCTGAGGATCAGCACTGCCAGTGGAGATGGGCGTCACTACTGCTACCCTCATTTCACCTGCGCTGTGGACAC
TGAGAACATCCGCCGTGTGTTCAACGACTGCCGTGACATCATTCAGCGCATGCACCTTCGTCAGTACGAGCTGCTCTAAGAAGGGAACCC
CCAAATTTAATTAAAGCCTTAAGCACAATTAATTAAAAGTGAAACGTAATTGTACAAGCAGTTAATCACCCACCATAGGGCATGATTAAC
AAAGCAACCTTTCCCTTCCCCCGAGTGATTTTGCGAAACCCCCTTTTCCCTTCAGCTTGCTTAGATGTTCCAAATTTAGAAAGCTTAAGG
CGGCCTACAGAAAAAGGAAAAAAGGCCACAAAAGTTCCCTCTCACTTTCAGTAAAAATAAATAAAACAGCAGCAGCAAACAAATAAAATG
AAATAAAAGAAACAAATGAAATAAATATTGTGTTGTGCAGCATTAAAAAAAATCAAAATAAAAATTAAATGTGAGCAAAGAAAAAGAAAA
AGATGACAAGTGGAAAAGAGGGAGCGAACCAGTTCCAGAAAAAAAATTGGAACCTGTTGTTTTTGAGAAGGTGAAAATGCCACAGAAAAA
AGAAGACCCACAGCTACCTCGGAAAAGCTCCCCGAAATCCACAGCGCCTGTCATGGATTTGTTGGGCCTTGATGCTCCTGTGGCCTGCTC
CATTGCAAATAGTAAGACCAGCAATACCCTAGAGAAGGATTTAGATCTGTTGGCCTCTGTTCCATCCCCTTCTTCTTCCGGTTCCAGAAA
GGTTGTAGGTTCCATGCCAACTGCAGGGAGTGCCGGCTCTGTTCCTGAAAATCTGAACCTGTTTCCGGAGCCAGGGAGCAAATCAGAAGA
AATAGGCAAGAAACAGCTCTCTAAAGACTCCATTCTTTCACTGTATGGATCCCAGACGCCTCAAATGCCTACTCAAGCAATGTTCATGGC
TCCCGCTCAGATGGCATATCCCACAGCCTACCCCAGCTTCCCCGGGGTTACACCTCCTAACAGCATAATGGGGAGCATGATGCCTCCACC
AGTAGGCATGGTTGCTCAGCCAGGAGCTTCTGGGATGGTTGCCCCCATGGCCATGCCTGCAGGCTATATGGGTGGCATGCAGGCATCAAT
GATGGGTGTGCCGAATGGAATGATGACCACCCAGCAGGCTGGCTACATGGCAGGCATGGCAGCTATGCCCCAGACTGTGTATGGGGTCCA
GCCAGCTCAGCAGCTGCAATGGAACCTTACTCAGATGACCCAGCAGATGGCTGGGATGAACTTCTATGGAGCCAATGGCATGATGAACTA
TGGACAGTCAATGAGTGGCGGAAATGGACAGGCAGCAAATCAGACTCTCAGTCCTCAGATGTGGAAATAAAAACAAAACACCTGTATGGC
TGCCATTCTCTTCAGCCCTCGCTCTCCCCTTTCCACAGCCTCCACCCCTGACCCCCATCCTCTTTTCCTACCTCTCTGTTTGGTTTAGAA

>In-frame_ENST00000371085_ENST00000539317_TCGA-H7-7774-01A_GNAS_chr20_57486241_-_SMAP2_chr1_40878688_length(amino acids)=541AA_start in transcript=1623_stop in transcript=1
MGVPFLEQLVLTKVHALNDVTAVVEHTADVLSVHSAGEMRVAVVTPISTGSADPQKLISNEVLGPGHAWVLSGLGSSILRSSVASKFWKV
VLNLRFPSKDFLSEQILLVEEQDHRDGAQPSVVPDALEEVQSLLQAVGLVVLPDDHVVAAAGHHEDDGSHIVEALDPLAAFIALATHVKH
VEVDFVHLELGLKDSRSQDTAAKQVLIARHIVSLLDHVDLVQEVLGTVNQLVLVGAFVAGTHSFILPQSLGMLIEFGREVKVRHVHHTQD
VVHSELVLGVGQLHGGHQVAHGGHNGFNRLFQVVFDVLHFGCLLTIAVAPCSLRVLFAALSIKPINMQDPHLLHNGAFTRFSSTQQQQAV
RGPVDLLVLLQLLLDLFVGLTLRLLLVALVLGLTVPEAAHGGGGGGGARPGCGGGGRGRPHAGREGRGSAGRAGRARARLLGKERAGRTA
GRGPRGARTGGKGRVSVGPRGARSSPPLEPRPRGLMAAAGPRRTRAGAAGAAAAAAAAAALIASAGPPPPPRAKSAAAGGGRRGEEEAAA

--------------------------------------------------------------
>In-frame_ENST00000354359_ENST00000539317_TCGA-H7-7774-01A_GNAS_chr20_57486241_-_SMAP2_chr1_40878688_length(transcript)=3022nt_BP=1975nt
TCGCCGCCGCCGCCTCCTCCTCCCCCCGCCTCCCCCCGCCCGCCGCGGCGCTTTTGGCTCGGGGCGGCGGCGGGGGCCCGGCCGAGGCAA
TAAGAGCGGCGGCGGCGGCAGCGGCGGCAGCAGCTCCCGCAGCTCCTGCTCTGGTCCGCCTCGGCCCGGCGGCGGCCATCAGCCCCCTCG
GCCTCGGCTCGAGGGGCGGGGAGCTGCGCGCGCCCCTCGGTCCGACCGACACCCTCCCCTTCCCGCCCGTCCGCGCGCCCCGCGGCCCGC
GGCCCGCAGTCCGCCCCGCGCGCTCCTTGCCGAGGAGCCGAGCCCGCGCCCGGCCCGCCCGCCCGGCGCTGCCCCGGCCCTCCCGGCCCG
CGTGAGGCCGCCCGCGCCCGCCGCCGCCGCAGCCCGGCCGCGCCCCGCCGCCGCCGCCGCCGCCATGGGCTGCCTCGGGAACAGTAAGAC
CGAGGACCAGCGCAACGAGGAGAAGGCGCAGCGTGAGGCCAACAAAAAGATCGAGAAGCAGCTGCAGAAGGACAAGCAGGTCTACCGGGC
CACGCACCGCCTGCTGCTGCTGGGTGCTGGAGAATCTGGTAAAAGCACCATTGTGAAGCAGATGAGGATCCTGCATGTTAATGGGTTTAA
TGGAGAGGGCGGCGAAGAGGACCCGCAGGCTGCAAGGAGCAACAGCGATGGCAGTGAGAAGGCAACCAAAGTGCAGGACATCAAAAACAA
CCTGAAAGAGGCGATTGAAACCATTGTGGCCGCCATGAGCAACCTGGTGCCCCCCGTGGAGCTGGCCAACCCCGAGAACCAGTTCAGAGT
GGACTACATCCTGAGTGTGATGAACGTGCCTGACTTTGACTTCCCTCCCGAATTCTATGAGCATGCCAAGGCTCTGTGGGAGGATGAAGG
AGTGCGTGCCTGCTACGAACGCTCCAACGAGTACCAGCTGATTGACTGTGCCCAGTACTTCCTGGACAAGATCGACGTGATCAAGCAGGC
TGACTATGTGCCGAGCGATCAGGACCTGCTTCGCTGCCGTGTCCTGACTTCTGGAATCTTTGAGACCAAGTTCCAGGTGGACAAAGTCAA
CTTCCACATGTTTGACGTGGGTGGCCAGCGCGATGAACGCCGCAAGTGGATCCAGTGCTTCAACGATGTGACTGCCATCATCTTCGTGGT
GGCCAGCAGCAGCTACAACATGGTCATCCGGGAGGACAACCAGACCAACCGCCTGCAGGAGGCTCTGAACCTCTTCAAGAGCATCTGGAA
CAACAGATGGCTGCGCACCATCTCTGTGATCCTGTTCCTCAACAAGCAAGATCTGCTCGCTGAGAAAGTCCTTGCTGGGAAATCGAAGAT
TGAGGACTACTTTCCAGAATTTGCTCGCTACACTACTCCTGAGGATGCTACTCCCGAGCCCGGAGAGGACCCACGCGTGACCCGGGCCAA
GTACTTCATTCGAGATGAGTTTCTGAGGATCAGCACTGCCAGTGGAGATGGGCGTCACTACTGCTACCCTCATTTCACCTGCGCTGTGGA
CACTGAGAACATCCGCCGTGTGTTCAACGACTGCCGTGACATCATTCAGCGCATGCACCTTCGTCAGTACGAGCTGCTCTAAGAAGGGAA
CCCCCAAATTTAATTAAAGCCTTAAGCACAATTAATTAAAAGTGAAACGTAATTGTACAAGCAGTTAATCACCCACCATAGGGCATGATT
AACAAAGCAACCTTTCCCTTCCCCCGAGTGATTTTGCGAAACCCCCTTTTCCCTTCAGCTTGCTTAGATGTTCCAAATTTAGAAAGCTTA
AGGCGGCCTACAGAAAAAGGAAAAAAGGCCACAAAAGTTCCCTCTCACTTTCAGTAAAAATAAATAAAACAGCAGCAGCAAACAAATAAA
ATGAAATAAAAGAAACAAATGAAATAAATATTGTGTTGTGCAGCATTAAAAAAAATCAAAATAAAAATTAAATGTGAGCAAAGAAAAAGA
AAAAGATGACAAGTGGAAAAGAGGGAGCGAACCAGTTCCAGAAAAAAAATTGGAACCTGTTGTTTTTGAGAAGGTGAAAATGCCACAGAA
AAAAGAAGACCCACAGCTACCTCGGAAAAGCTCCCCGAAATCCACAGCGCCTGTCATGGATTTGTTGGGCCTTGATGCTCCTGTGGCCTG
CTCCATTGCAAATAGTAAGACCAGCAATACCCTAGAGAAGGATTTAGATCTGTTGGCCTCTGTTCCATCCCCTTCTTCTTCCGGTTCCAG
AAAGGTTGTAGGTTCCATGCCAACTGCAGGGAGTGCCGGCTCTGTTCCTGAAAATCTGAACCTGTTTCCGGAGCCAGGGAGCAAATCAGA
AGAAATAGGCAAGAAACAGCTCTCTAAAGACTCCATTCTTTCACTGTATGGATCCCAGACGCCTCAAATGCCTACTCAAGCAATGTTCAT
GGCTCCCGCTCAGATGGCATATCCCACAGCCTACCCCAGCTTCCCCGGGGTTACACCTCCTAACAGCATAATGGGGAGCATGATGCCTCC
ACCAGTAGGCATGGTTGCTCAGCCAGGAGCTTCTGGGATGGTTGCCCCCATGGCCATGCCTGCAGGCTATATGGGTGGCATGCAGGCATC
AATGATGGGTGTGCCGAATGGAATGATGACCACCCAGCAGGCTGGCTACATGGCAGGCATGGCAGCTATGCCCCAGACTGTGTATGGGGT
CCAGCCAGCTCAGCAGCTGCAATGGAACCTTACTCAGATGACCCAGCAGATGGCTGGGATGAACTTCTATGGAGCCAATGGCATGATGAA
CTATGGACAGTCAATGAGTGGCGGAAATGGACAGGCAGCAAATCAGACTCTCAGTCCTCAGATGTGGAAATAAAAACAAAACACCTGTAT
GGCTGCCATTCTCTTCAGCCCTCGCTCTCCCCTTTCCACAGCCTCCACCCCTGACCCCCATCCTCTTTTCCTACCTCTCTGTTTGGTTTA

>In-frame_ENST00000354359_ENST00000539317_TCGA-H7-7774-01A_GNAS_chr20_57486241_-_SMAP2_chr1_40878688_length(amino acids)=542AA_start in transcript=1626_stop in transcript=1
MGVPFLEQLVLTKVHALNDVTAVVEHTADVLSVHSAGEMRVAVVTPISTGSADPQKLISNEVLGPGHAWVLSGLGSSILRSSVASKFWKV
VLNLRFPSKDFLSEQILLVEEQDHRDGAQPSVVPDALEEVQSLLQAVGLVVLPDDHVVAAAGHHEDDGSHIVEALDPLAAFIALATHVKH
VEVDFVHLELGLKDSRSQDTAAKQVLIARHIVSLLDHVDLVQEVLGTVNQLVLVGAFVAGTHSFILPQSLGMLIEFGREVKVRHVHHTQD
VVHSELVLGVGQLHGGHQVAHGGHNGFNRLFQVVFDVLHFGCLLTAIAVAPCSLRVLFAALSIKPINMQDPHLLHNGAFTRFSSTQQQQA
VRGPVDLLVLLQLLLDLFVGLTLRLLLVALVLGLTVPEAAHGGGGGGGARPGCGGGGRGRPHAGREGRGSAGRAGRARARLLGKERAGRT
AGRGPRGARTGGKGRVSVGPRGARSSPPLEPRPRGLMAAAGPRRTRAGAAGAAAAAAAAAALIASAGPPPPPRAKSAAAGGGRRGEEEAA

--------------------------------------------------------------
>In-frame_ENST00000265620_ENST00000539317_TCGA-H7-7774-01A_GNAS_chr20_57486241_-_SMAP2_chr1_40878688_length(transcript)=2913nt_BP=1866nt
GGGCGGCGGCGGGGGCCCGGCCGAGGCAATAAGAGCGGCGGCGGCGGCAGCGGCGGCAGCAGCTCCCGCAGCTCCTGCTCTGGTCCGCCT
CGGCCCGGCGGCGGCCATCAGCCCCCTCGGCCTCGGCTCGAGGGGCGGGGAGCTGCGCGCGCCCCTCGGTCCGACCGACACCCTCCCCTT
CCCGCCCGTCCGCGCGCCCCGCGGCCCGCGGCCCGCAGTCCGCCCCGCGCGCTCCTTGCCGAGGAGCCGAGCCCGCGCCCGGCCCGCCCG
CCCGGCGCTGCCCCGGCCCTCCCGGCCCGCGTGAGGCCGCCCGCGCCCGCCGCCGCCGCAGCCCGGCCGCGCCCCGCCGCCGCCGCCGCC
GCCATGGGCTGCCTCGGGAACAGTAAGACCGAGGACCAGCGCAACGAGGAGAAGGCGCAGCGTGAGGCCAACAAAAAGATCGAGAAGCAG
CTGCAGAAGGACAAGCAGGTCTACCGGGCCACGCACCGCCTGCTGCTGCTGGGTGCTGGAGAATCTGGTAAAAGCACCATTGTGAAGCAG
ATGAGGATCCTGCATGTTAATGGGTTTAATGGAGATGAGAAGGCAACCAAAGTGCAGGACATCAAAAACAACCTGAAAGAGGCGATTGAA
ACCATTGTGGCCGCCATGAGCAACCTGGTGCCCCCCGTGGAGCTGGCCAACCCCGAGAACCAGTTCAGAGTGGACTACATCCTGAGTGTG
ATGAACGTGCCTGACTTTGACTTCCCTCCCGAATTCTATGAGCATGCCAAGGCTCTGTGGGAGGATGAAGGAGTGCGTGCCTGCTACGAA
CGCTCCAACGAGTACCAGCTGATTGACTGTGCCCAGTACTTCCTGGACAAGATCGACGTGATCAAGCAGGCTGACTATGTGCCGAGCGAT
CAGGACCTGCTTCGCTGCCGTGTCCTGACTTCTGGAATCTTTGAGACCAAGTTCCAGGTGGACAAAGTCAACTTCCACATGTTTGACGTG
GGTGGCCAGCGCGATGAACGCCGCAAGTGGATCCAGTGCTTCAACGATGTGACTGCCATCATCTTCGTGGTGGCCAGCAGCAGCTACAAC
ATGGTCATCCGGGAGGACAACCAGACCAACCGCCTGCAGGAGGCTCTGAACCTCTTCAAGAGCATCTGGAACAACAGATGGCTGCGCACC
ATCTCTGTGATCCTGTTCCTCAACAAGCAAGATCTGCTCGCTGAGAAAGTCCTTGCTGGGAAATCGAAGATTGAGGACTACTTTCCAGAA
TTTGCTCGCTACACTACTCCTGAGGATGCTACTCCCGAGCCCGGAGAGGACCCACGCGTGACCCGGGCCAAGTACTTCATTCGAGATGAG
TTTCTGAGGATCAGCACTGCCAGTGGAGATGGGCGTCACTACTGCTACCCTCATTTCACCTGCGCTGTGGACACTGAGAACATCCGCCGT
GTGTTCAACGACTGCCGTGACATCATTCAGCGCATGCACCTTCGTCAGTACGAGCTGCTCTAAGAAGGGAACCCCCAAATTTAATTAAAG
CCTTAAGCACAATTAATTAAAAGTGAAACGTAATTGTACAAGCAGTTAATCACCCACCATAGGGCATGATTAACAAAGCAACCTTTCCCT
TCCCCCGAGTGATTTTGCGAAACCCCCTTTTCCCTTCAGCTTGCTTAGATGTTCCAAATTTAGAAAGCTTAAGGCGGCCTACAGAAAAAG
GAAAAAAGGCCACAAAAGTTCCCTCTCACTTTCAGTAAAAATAAATAAAACAGCAGCAGCAAACAAATAAAATGAAATAAAAGAAACAAA
TGAAATAAATATTGTGTTGTGCAGCATTAAAAAAAATCAAAATAAAAATTAAATGTGAGCAAAGAAAAAGAAAAAGATGACAAGTGGAAA
AGAGGGAGCGAACCAGTTCCAGAAAAAAAATTGGAACCTGTTGTTTTTGAGAAGGTGAAAATGCCACAGAAAAAAGAAGACCCACAGCTA
CCTCGGAAAAGCTCCCCGAAATCCACAGCGCCTGTCATGGATTTGTTGGGCCTTGATGCTCCTGTGGCCTGCTCCATTGCAAATAGTAAG
ACCAGCAATACCCTAGAGAAGGATTTAGATCTGTTGGCCTCTGTTCCATCCCCTTCTTCTTCCGGTTCCAGAAAGGTTGTAGGTTCCATG
CCAACTGCAGGGAGTGCCGGCTCTGTTCCTGAAAATCTGAACCTGTTTCCGGAGCCAGGGAGCAAATCAGAAGAAATAGGCAAGAAACAG
CTCTCTAAAGACTCCATTCTTTCACTGTATGGATCCCAGACGCCTCAAATGCCTACTCAAGCAATGTTCATGGCTCCCGCTCAGATGGCA
TATCCCACAGCCTACCCCAGCTTCCCCGGGGTTACACCTCCTAACAGCATAATGGGGAGCATGATGCCTCCACCAGTAGGCATGGTTGCT
CAGCCAGGAGCTTCTGGGATGGTTGCCCCCATGGCCATGCCTGCAGGCTATATGGGTGGCATGCAGGCATCAATGATGGGTGTGCCGAAT
GGAATGATGACCACCCAGCAGGCTGGCTACATGGCAGGCATGGCAGCTATGCCCCAGACTGTGTATGGGGTCCAGCCAGCTCAGCAGCTG
CAATGGAACCTTACTCAGATGACCCAGCAGATGGCTGGGATGAACTTCTATGGAGCCAATGGCATGATGAACTATGGACAGTCAATGAGT
GGCGGAAATGGACAGGCAGCAAATCAGACTCTCAGTCCTCAGATGTGGAAATAAAAACAAAACACCTGTATGGCTGCCATTCTCTTCAGC
CCTCGCTCTCCCCTTTCCACAGCCTCCACCCCTGACCCCCATCCTCTTTTCCTACCTCTCTGTTTGGTTTAGAAATTGCTCAATAAGTCA

>In-frame_ENST00000265620_ENST00000539317_TCGA-H7-7774-01A_GNAS_chr20_57486241_-_SMAP2_chr1_40878688_length(amino acids)=506AA_start in transcript=1517_stop in transcript=0
MGVPFLEQLVLTKVHALNDVTAVVEHTADVLSVHSAGEMRVAVVTPISTGSADPQKLISNEVLGPGHAWVLSGLGSSILRSSVASKFWKV
VLNLRFPSKDFLSEQILLVEEQDHRDGAQPSVVPDALEEVQSLLQAVGLVVLPDDHVVAAAGHHEDDGSHIVEALDPLAAFIALATHVKH
VEVDFVHLELGLKDSRSQDTAAKQVLIARHIVSLLDHVDLVQEVLGTVNQLVLVGAFVAGTHSFILPQSLGMLIEFGREVKVRHVHHTQD
VVHSELVLGVGQLHGGHQVAHGGHNGFNRLFQVVFDVLHFGCLLISIKPINMQDPHLLHNGAFTRFSSTQQQQAVRGPVDLLVLLQLLLD
LFVGLTLRLLLVALVLGLTVPEAAHGGGGGGGARPGCGGGGRGRPHAGREGRGSAGRAGRARARLLGKERAGRTAGRGPRGARTGGKGRV

--------------------------------------------------------------
>In-frame_ENST00000306090_ENST00000539317_TCGA-H7-7774-01A_GNAS_chr20_57486241_-_SMAP2_chr1_40878688_length(transcript)=2689nt_BP=1642nt
CAGCGGCGGCAGCAGCTCCCGCAGCTCCTGCTCTGGTCCGCCTCGGCCCGGCGGCGGCCATCAGCCCCCTCGGCCTCGGCTCGAGGGGCG
GGGAGCTGCGCGCGCCCCTCGGTCCGACCGACACCCTCCCCTTCCCGCCCCCATGGGCTGCCTCGGGAACAGTAAGACCGAGGACCAGCG
CAACGAGGAGAAGGCGCAGCGTGAGGCCAACAAAAAGATCGAGAAGCAGCTGCAGAAGGACAAGCAGGTCTACCGGGCCACGCACCGCCT
GCTGCTGCTGGGTGCTGGAGAATCTGGTAAAAGCACCATTGTGAAGCAGATGAGGATCCTGCATGTTAATGGGTTTAATGGAGACAGTGA
GAAGGCAACCAAAGTGCAGGACATCAAAAACAACCTGAAAGAGGCGATTGAAACCATTGTGGCCGCCATGAGCAACCTGGTGCCCCCCGT
GGAGCTGGCCAACCCCGAGAACCAGTTCAGAGTGGACTACATCCTGAGTGTGATGAACGTGCCTGACTTTGACTTCCCTCCCGAATTCTA
TGAGCATGCCAAGGCTCTGTGGGAGGATGAAGGAGTGCGTGCCTGCTACGAACGCTCCAACGAGTACCAGCTGATTGACTGTGCCCAGTA
CTTCCTGGACAAGATCGACGTGATCAAGCAGGCTGACTATGTGCCGAGCGATCAGGACCTGCTTCGCTGCCGTGTCCTGACTTCTGGAAT
CTTTGAGACCAAGTTCCAGGTGGACAAAGTCAACTTCCACATGTTTGACGTGGGTGGCCAGCGCGATGAACGCCGCAAGTGGATCCAGTG
CTTCAACGATGTGACTGCCATCATCTTCGTGGTGGCCAGCAGCAGCTACAACATGGTCATCCGGGAGGACAACCAGACCAACCGCCTGCA
GGAGGCTCTGAACCTCTTCAAGAGCATCTGGAACAACAGATGGCTGCGCACCATCTCTGTGATCCTGTTCCTCAACAAGCAAGATCTGCT
CGCTGAGAAAGTCCTTGCTGGGAAATCGAAGATTGAGGACTACTTTCCAGAATTTGCTCGCTACACTACTCCTGAGGATGCTACTCCCGA
GCCCGGAGAGGACCCACGCGTGACCCGGGCCAAGTACTTCATTCGAGATGAGTTTCTGAGGATCAGCACTGCCAGTGGAGATGGGCGTCA
CTACTGCTACCCTCATTTCACCTGCGCTGTGGACACTGAGAACATCCGCCGTGTGTTCAACGACTGCCGTGACATCATTCAGCGCATGCA
CCTTCGTCAGTACGAGCTGCTCTAAGAAGGGAACCCCCAAATTTAATTAAAGCCTTAAGCACAATTAATTAAAAGTGAAACGTAATTGTA
CAAGCAGTTAATCACCCACCATAGGGCATGATTAACAAAGCAACCTTTCCCTTCCCCCGAGTGATTTTGCGAAACCCCCTTTTCCCTTCA
GCTTGCTTAGATGTTCCAAATTTAGAAAGCTTAAGGCGGCCTACAGAAAAAGGAAAAAAGGCCACAAAAGTTCCCTCTCACTTTCAGTAA
AAATAAATAAAACAGCAGCAGCAAACAAATAAAATGAAATAAAAGAAACAAATGAAATAAATATTGTGTTGTGCAGCATTAAAAAAAATC
AAAATAAAAATTAAATGTGAGCAAAGAAAAAGATGACAAGTGGAAAAGAGGGAGCGAACCAGTTCCAGAAAAAAAATTGGAACCTGTTGT
TTTTGAGAAGGTGAAAATGCCACAGAAAAAAGAAGACCCACAGCTACCTCGGAAAAGCTCCCCGAAATCCACAGCGCCTGTCATGGATTT
GTTGGGCCTTGATGCTCCTGTGGCCTGCTCCATTGCAAATAGTAAGACCAGCAATACCCTAGAGAAGGATTTAGATCTGTTGGCCTCTGT
TCCATCCCCTTCTTCTTCCGGTTCCAGAAAGGTTGTAGGTTCCATGCCAACTGCAGGGAGTGCCGGCTCTGTTCCTGAAAATCTGAACCT
GTTTCCGGAGCCAGGGAGCAAATCAGAAGAAATAGGCAAGAAACAGCTCTCTAAAGACTCCATTCTTTCACTGTATGGATCCCAGACGCC
TCAAATGCCTACTCAAGCAATGTTCATGGCTCCCGCTCAGATGGCATATCCCACAGCCTACCCCAGCTTCCCCGGGGTTACACCTCCTAA
CAGCATAATGGGGAGCATGATGCCTCCACCAGTAGGCATGGTTGCTCAGCCAGGAGCTTCTGGGATGGTTGCCCCCATGGCCATGCCTGC
AGGCTATATGGGTGGCATGCAGGCATCAATGATGGGTGTGCCGAATGGAATGATGACCACCCAGCAGGCTGGCTACATGGCAGGCATGGC
AGCTATGCCCCAGACTGTGTATGGGGTCCAGCCAGCTCAGCAGCTGCAATGGAACCTTACTCAGATGACCCAGCAGATGGCTGGGATGAA
CTTCTATGGAGCCAATGGCATGATGAACTATGGACAGTCAATGAGTGGCGGAAATGGACAGGCAGCAAATCAGACTCTCAGTCCTCAGAT
GTGGAAATAAAAACAAAACACCTGTATGGCTGCCATTCTCTTCAGCCCTCGCTCTCCCCTTTCCACAGCCTCCACCCCTGACCCCCATCC

>In-frame_ENST00000306090_ENST00000539317_TCGA-H7-7774-01A_GNAS_chr20_57486241_-_SMAP2_chr1_40878688_length(amino acids)=433AA_start in transcript=1299_stop in transcript=1
MGVPFLEQLVLTKVHALNDVTAVVEHTADVLSVHSAGEMRVAVVTPISTGSADPQKLISNEVLGPGHAWVLSGLGSSILRSSVASKFWKV
VLNLRFPSKDFLSEQILLVEEQDHRDGAQPSVVPDALEEVQSLLQAVGLVVLPDDHVVAAAGHHEDDGSHIVEALDPLAAFIALATHVKH
VEVDFVHLELGLKDSRSQDTAAKQVLIARHIVSLLDHVDLVQEVLGTVNQLVLVGAFVAGTHSFILPQSLGMLIEFGREVKVRHVHHTQD
VVHSELVLGVGQLHGGHQVAHGGHNGFNRLFQVVFDVLHFGCLLTVSIKPINMQDPHLLHNGAFTRFSSTQQQQAVRGPVDLLVLLQLLL

--------------------------------------------------------------

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Fusion Gene PPI Analysis for GNAS-SMAP2


check button Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in

ChiPPI page.


check button Protein-protein interactors with each fusion partner protein in wild-type (BIOGRID-3.4.160)
HgeneHgene's interactorsTgeneTgene's interactors


check button - Retained PPIs in in-frame fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenStill interaction with
TgeneSMAP2chr20:57486241chr1:40878688ENST00000539317310163_23254.0350.0clathrin heavy chains
TgeneSMAP2chr20:57486241chr1:40878688ENST00000539317310340_42954.0350.0PICALM


check button - Lost PPIs in in-frame fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenInteraction lost with


check button - Retained PPIs, but lost function due to frame-shift fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenInteraction lost with


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Related Drugs for GNAS-SMAP2


check button Drugs targeting genes involved in this fusion gene.
(DrugBank Version 5.1.8 2021-05-08)
PartnerGeneUniProtAccDrugBank IDDrug nameDrug activityDrug typeDrug status

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Related Diseases for GNAS-SMAP2


check button Diseases associated with fusion partners.
(DisGeNet 4.0)
PartnerGeneDisease IDDisease name# pubmedsSource
HgeneGNASC3494506Pseudohypoparathyroidism, Type Ia17CTD_human;GENOMICS_ENGLAND;ORPHANET;UNIPROT
HgeneGNASC0242292McCune-Albright Syndrome7CTD_human;GENOMICS_ENGLAND;ORPHANET;UNIPROT
HgeneGNASC0016065Polyostotic fibrous dysplasia5CTD_human;ORPHANET
HgeneGNASC1864100PSEUDOHYPOPARATHYROIDISM, TYPE IB5CTD_human;GENOMICS_ENGLAND;ORPHANET
HgeneGNASC2931404Albright's hereditary osteodystrophy4CTD_human;GENOMICS_ENGLAND
HgeneGNASC0033806Pseudohypoparathyroidism3CTD_human
HgeneGNASC0334041Osteoma cutis3CTD_human;GENOMICS_ENGLAND;ORPHANET;UNIPROT
HgeneGNASC0014130Endocrine System Diseases2CTD_human
HgeneGNASC0016064Fibrous Dysplasia, Monostotic2ORPHANET
HgeneGNASC0034013Precocious Puberty2CTD_human
HgeneGNASC0221263Cafe-au-Lait Spots2CTD_human
HgeneGNASC0271527Cryptogenic sexual precocity2CTD_human
HgeneGNASC0342543Central Precocious Puberty2CTD_human
HgeneGNASC1504412Testotoxicosis2CTD_human
HgeneGNASC1857451Acth-Independent Macronodular Adrenal Hyperplasia2CTD_human;GENOMICS_ENGLAND;ORPHANET;UNIPROT
HgeneGNASC2932716Pseudohypoparathyroidism Type 1C2GENOMICS_ENGLAND;ORPHANET;UNIPROT
HgeneGNASC0001206Acromegaly1CTD_human
HgeneGNASC0001624Adrenal Gland Neoplasms1CTD_human
HgeneGNASC0003129Anoxemia1CTD_human
HgeneGNASC0003130Anoxia1CTD_human
HgeneGNASC0008370Cholestasis1GENOMICS_ENGLAND
HgeneGNASC0009438Common Bile Duct Calculi1CTD_human
HgeneGNASC0011573Endogenous depression1PSYGENET
HgeneGNASC0019087Hemorrhagic Disorders1CTD_human
HgeneGNASC0020538Hypertensive disease1CTD_human
HgeneGNASC0020796Profound Mental Retardation1CTD_human
HgeneGNASC0021655Insulin Resistance1CTD_human
HgeneGNASC0023897Liver Diseases, Parasitic1CTD_human
HgeneGNASC0025363Mental Retardation, Psychosocial1CTD_human
HgeneGNASC0027819Neuroblastoma1CTD_human
HgeneGNASC0028754Obesity1CTD_human
HgeneGNASC0029396Heterotopic Ossification1CTD_human
HgeneGNASC0032460Polycystic Ovary Syndrome1CTD_human
HgeneGNASC0033835Pseudopseudohypoparathyroidism1CTD_human;GENOMICS_ENGLAND;ORPHANET
HgeneGNASC0035204Respiration Disorders1CTD_human
HgeneGNASC0036341Schizophrenia1PSYGENET
HgeneGNASC0038587Substance Withdrawal Syndrome1CTD_human
HgeneGNASC0039231Tachycardia1CTD_human
HgeneGNASC0080203Tachyarrhythmia1CTD_human
HgeneGNASC0086189Drug Withdrawal Symptoms1CTD_human
HgeneGNASC0087169Withdrawal Symptoms1CTD_human
HgeneGNASC0206698Cholangiocarcinoma1CTD_human
HgeneGNASC0221357Brachydactyly1CTD_human
HgeneGNASC0242184Hypoxia1CTD_human
HgeneGNASC0242216Biliary calculi1CTD_human
HgeneGNASC0345905Intrahepatic Cholangiocarcinoma1CTD_human
HgeneGNASC0346302Growth Hormone-Secreting Pituitary Adenoma1CTD_human
HgeneGNASC0700292Hypoxemia1CTD_human
HgeneGNASC0750887Adrenal Cancer1CTD_human
HgeneGNASC0917816Mental deficiency1CTD_human
HgeneGNASC0920563Insulin Sensitivity1CTD_human
HgeneGNASC1136382Sclerocystic Ovaries1CTD_human
HgeneGNASC2932715Pseudohypoparathyroidism Type 1B1CTD_human;GENOMICS_ENGLAND;ORPHANET
HgeneGNASC3489630Somatotrophinoma, Familial1CTD_human
HgeneGNASC3697137Fibrous dysplasia of bone with intramuscular myxoma1ORPHANET
HgeneGNASC3714756Intellectual Disability1CTD_human
HgeneGNASC3805278Extrahepatic Cholangiocarcinoma1CTD_human