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Fusion Gene Summary | |
Fusion Gene ORF analysis | |
Fusion Genomic Features | |
Fusion Protein Features | |
Fusion Gene Sequence | |
Fusion Gene PPI analysis | |
Related Drugs | |
Related Diseases |
Fusion gene:GRB10-MAD1L1 (FusionGDB2 ID:34498) |
Fusion Gene Summary for GRB10-MAD1L1 |
Fusion gene summary |
Fusion gene information | Fusion gene name: GRB10-MAD1L1 | Fusion gene ID: 34498 | Hgene | Tgene | Gene symbol | GRB10 | MAD1L1 | Gene ID | 2887 | 8379 |
Gene name | growth factor receptor bound protein 10 | mitotic arrest deficient 1 like 1 | |
Synonyms | GRB-IR|Grb-10|IRBP|MEG1|RSS | MAD1|PIG9|TP53I9|TXBP181 | |
Cytomap | 7p12.1 | 7p22.3 | |
Type of gene | protein-coding | protein-coding | |
Description | growth factor receptor-bound protein 10GRB10 adapter proteinGRB10 adaptor proteininsulin receptor-binding protein Grb-IRmaternally expressed gene 1 | mitotic spindle assembly checkpoint protein MAD1MAD1 mitotic arrest deficient like 1MAD1-like protein 1mitotic arrest deficient 1-like protein 1mitotic checkpoint MAD1 protein homologmitotic-arrest deficient 1, yeast, homolog-like 1tax-binding prote | |
Modification date | 20200327 | 20200313 | |
UniProtAcc | Q13322 | Q9Y6D9 | |
Ensembl transtripts involved in fusion gene | ENST00000398810, ENST00000439599, ENST00000398812, ENST00000335866, ENST00000402578, ENST00000403097, ENST00000406641, ENST00000357271, ENST00000407526, ENST00000401949, ENST00000402497, ENST00000483819, | ENST00000402746, ENST00000399654, ENST00000406869, ENST00000265854, ENST00000486340, | |
Fusion gene scores | * DoF score | 13 X 10 X 8=1040 | 23 X 16 X 12=4416 |
# samples | 16 | 37 | |
** MAII score | log2(16/1040*10)=-2.70043971814109 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0 | log2(37/4416*10)=-3.57714299626186 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0 | |
Context | PubMed: GRB10 [Title/Abstract] AND MAD1L1 [Title/Abstract] AND fusion [Title/Abstract] | ||
Most frequent breakpoint | GRB10(50823584)-MAD1L1(2188873), # samples:3 | ||
Anticipated loss of major functional domain due to fusion event. | GRB10-MAD1L1 seems lost the major protein functional domain in Hgene partner, which is a essential gene due to the frame-shifted ORF. GRB10-MAD1L1 seems lost the major protein functional domain in Tgene partner, which is a tumor suppressor due to the frame-shifted ORF. GRB10-MAD1L1 seems lost the major protein functional domain in Tgene partner, which is a essential gene due to the frame-shifted ORF. |
* DoF score (Degree of Frequency) = # partners X # break points X # cancer types ** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10) |
Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez |
Partner | Gene | GO ID | GO term | PubMed ID |
Hgene | GRB10 | GO:0030178 | negative regulation of Wnt signaling pathway | 17376403 |
Hgene | GRB10 | GO:0030949 | positive regulation of vascular endothelial growth factor receptor signaling pathway | 15060076 |
Tgene | MAD1L1 | GO:0007094 | mitotic spindle assembly checkpoint | 18981471 |
Tgene | MAD1L1 | GO:0090235 | regulation of metaphase plate congression | 20133940 |
Fusion gene breakpoints across GRB10 (5'-gene) * Click on the image to open the UCSC genome browser with custom track showing this image in a new window. |
Fusion gene breakpoints across MAD1L1 (3'-gene) * Click on the image to open the UCSC genome browser with custom track showing this image in a new window. |
Fusion gene information from two resources (ChiTars 5.0 and ChimerDB 4.0) * All genome coordinats were lifted-over on hg19. * Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser. |
Source | Disease | Sample | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand |
ChimerDB4 | LUAD | TCGA-05-4415-01A | GRB10 | chr7 | 50823584 | - | MAD1L1 | chr7 | 2188873 | - |
ChimerDB4 | LUAD | TCGA-05-4415-01A | GRB10 | chr7 | 50823584 | - | MAD1L1 | chr7 | 2188873 | - |
ChimerDB4 | LUAD | TCGA-05-4415-01A | GRB10 | chr7 | 50823584 | - | MAD1L1 | chr7 | 2188873 | - |
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Fusion Gene ORF analysis for GRB10-MAD1L1 |
Open reading frame (ORF) analsis of fusion genes based on Ensembl gene isoform structure. * Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser. |
ORF | Henst | Tenst | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand |
intron-3CDS | ENST00000398810 | ENST00000402746 | GRB10 | chr7 | 50823584 | - | MAD1L1 | chr7 | 2188873 | - |
intron-3CDS | ENST00000398810 | ENST00000399654 | GRB10 | chr7 | 50823584 | - | MAD1L1 | chr7 | 2188873 | - |
intron-3CDS | ENST00000398810 | ENST00000406869 | GRB10 | chr7 | 50823584 | - | MAD1L1 | chr7 | 2188873 | - |
intron-3CDS | ENST00000398810 | ENST00000265854 | GRB10 | chr7 | 50823584 | - | MAD1L1 | chr7 | 2188873 | - |
intron-intron | ENST00000398810 | ENST00000486340 | GRB10 | chr7 | 50823584 | - | MAD1L1 | chr7 | 2188873 | - |
intron-3CDS | ENST00000439599 | ENST00000402746 | GRB10 | chr7 | 50823584 | - | MAD1L1 | chr7 | 2188873 | - |
intron-3CDS | ENST00000439599 | ENST00000399654 | GRB10 | chr7 | 50823584 | - | MAD1L1 | chr7 | 2188873 | - |
intron-3CDS | ENST00000439599 | ENST00000406869 | GRB10 | chr7 | 50823584 | - | MAD1L1 | chr7 | 2188873 | - |
intron-3CDS | ENST00000439599 | ENST00000265854 | GRB10 | chr7 | 50823584 | - | MAD1L1 | chr7 | 2188873 | - |
intron-intron | ENST00000439599 | ENST00000486340 | GRB10 | chr7 | 50823584 | - | MAD1L1 | chr7 | 2188873 | - |
intron-3CDS | ENST00000398812 | ENST00000402746 | GRB10 | chr7 | 50823584 | - | MAD1L1 | chr7 | 2188873 | - |
intron-3CDS | ENST00000398812 | ENST00000399654 | GRB10 | chr7 | 50823584 | - | MAD1L1 | chr7 | 2188873 | - |
intron-3CDS | ENST00000398812 | ENST00000406869 | GRB10 | chr7 | 50823584 | - | MAD1L1 | chr7 | 2188873 | - |
intron-3CDS | ENST00000398812 | ENST00000265854 | GRB10 | chr7 | 50823584 | - | MAD1L1 | chr7 | 2188873 | - |
intron-intron | ENST00000398812 | ENST00000486340 | GRB10 | chr7 | 50823584 | - | MAD1L1 | chr7 | 2188873 | - |
5UTR-3CDS | ENST00000335866 | ENST00000402746 | GRB10 | chr7 | 50823584 | - | MAD1L1 | chr7 | 2188873 | - |
5UTR-3CDS | ENST00000335866 | ENST00000399654 | GRB10 | chr7 | 50823584 | - | MAD1L1 | chr7 | 2188873 | - |
5UTR-3CDS | ENST00000335866 | ENST00000406869 | GRB10 | chr7 | 50823584 | - | MAD1L1 | chr7 | 2188873 | - |
5UTR-3CDS | ENST00000335866 | ENST00000265854 | GRB10 | chr7 | 50823584 | - | MAD1L1 | chr7 | 2188873 | - |
5UTR-intron | ENST00000335866 | ENST00000486340 | GRB10 | chr7 | 50823584 | - | MAD1L1 | chr7 | 2188873 | - |
intron-3CDS | ENST00000402578 | ENST00000402746 | GRB10 | chr7 | 50823584 | - | MAD1L1 | chr7 | 2188873 | - |
intron-3CDS | ENST00000402578 | ENST00000399654 | GRB10 | chr7 | 50823584 | - | MAD1L1 | chr7 | 2188873 | - |
intron-3CDS | ENST00000402578 | ENST00000406869 | GRB10 | chr7 | 50823584 | - | MAD1L1 | chr7 | 2188873 | - |
intron-3CDS | ENST00000402578 | ENST00000265854 | GRB10 | chr7 | 50823584 | - | MAD1L1 | chr7 | 2188873 | - |
intron-intron | ENST00000402578 | ENST00000486340 | GRB10 | chr7 | 50823584 | - | MAD1L1 | chr7 | 2188873 | - |
5UTR-3CDS | ENST00000403097 | ENST00000402746 | GRB10 | chr7 | 50823584 | - | MAD1L1 | chr7 | 2188873 | - |
5UTR-3CDS | ENST00000403097 | ENST00000399654 | GRB10 | chr7 | 50823584 | - | MAD1L1 | chr7 | 2188873 | - |
5UTR-3CDS | ENST00000403097 | ENST00000406869 | GRB10 | chr7 | 50823584 | - | MAD1L1 | chr7 | 2188873 | - |
5UTR-3CDS | ENST00000403097 | ENST00000265854 | GRB10 | chr7 | 50823584 | - | MAD1L1 | chr7 | 2188873 | - |
5UTR-intron | ENST00000403097 | ENST00000486340 | GRB10 | chr7 | 50823584 | - | MAD1L1 | chr7 | 2188873 | - |
5UTR-3CDS | ENST00000406641 | ENST00000402746 | GRB10 | chr7 | 50823584 | - | MAD1L1 | chr7 | 2188873 | - |
5UTR-3CDS | ENST00000406641 | ENST00000399654 | GRB10 | chr7 | 50823584 | - | MAD1L1 | chr7 | 2188873 | - |
5UTR-3CDS | ENST00000406641 | ENST00000406869 | GRB10 | chr7 | 50823584 | - | MAD1L1 | chr7 | 2188873 | - |
5UTR-3CDS | ENST00000406641 | ENST00000265854 | GRB10 | chr7 | 50823584 | - | MAD1L1 | chr7 | 2188873 | - |
5UTR-intron | ENST00000406641 | ENST00000486340 | GRB10 | chr7 | 50823584 | - | MAD1L1 | chr7 | 2188873 | - |
intron-3CDS | ENST00000357271 | ENST00000402746 | GRB10 | chr7 | 50823584 | - | MAD1L1 | chr7 | 2188873 | - |
intron-3CDS | ENST00000357271 | ENST00000399654 | GRB10 | chr7 | 50823584 | - | MAD1L1 | chr7 | 2188873 | - |
intron-3CDS | ENST00000357271 | ENST00000406869 | GRB10 | chr7 | 50823584 | - | MAD1L1 | chr7 | 2188873 | - |
intron-3CDS | ENST00000357271 | ENST00000265854 | GRB10 | chr7 | 50823584 | - | MAD1L1 | chr7 | 2188873 | - |
intron-intron | ENST00000357271 | ENST00000486340 | GRB10 | chr7 | 50823584 | - | MAD1L1 | chr7 | 2188873 | - |
5UTR-3CDS | ENST00000407526 | ENST00000402746 | GRB10 | chr7 | 50823584 | - | MAD1L1 | chr7 | 2188873 | - |
5UTR-3CDS | ENST00000407526 | ENST00000399654 | GRB10 | chr7 | 50823584 | - | MAD1L1 | chr7 | 2188873 | - |
5UTR-3CDS | ENST00000407526 | ENST00000406869 | GRB10 | chr7 | 50823584 | - | MAD1L1 | chr7 | 2188873 | - |
5UTR-3CDS | ENST00000407526 | ENST00000265854 | GRB10 | chr7 | 50823584 | - | MAD1L1 | chr7 | 2188873 | - |
5UTR-intron | ENST00000407526 | ENST00000486340 | GRB10 | chr7 | 50823584 | - | MAD1L1 | chr7 | 2188873 | - |
5UTR-3CDS | ENST00000401949 | ENST00000402746 | GRB10 | chr7 | 50823584 | - | MAD1L1 | chr7 | 2188873 | - |
5UTR-3CDS | ENST00000401949 | ENST00000399654 | GRB10 | chr7 | 50823584 | - | MAD1L1 | chr7 | 2188873 | - |
5UTR-3CDS | ENST00000401949 | ENST00000406869 | GRB10 | chr7 | 50823584 | - | MAD1L1 | chr7 | 2188873 | - |
5UTR-3CDS | ENST00000401949 | ENST00000265854 | GRB10 | chr7 | 50823584 | - | MAD1L1 | chr7 | 2188873 | - |
5UTR-intron | ENST00000401949 | ENST00000486340 | GRB10 | chr7 | 50823584 | - | MAD1L1 | chr7 | 2188873 | - |
intron-3CDS | ENST00000402497 | ENST00000402746 | GRB10 | chr7 | 50823584 | - | MAD1L1 | chr7 | 2188873 | - |
intron-3CDS | ENST00000402497 | ENST00000399654 | GRB10 | chr7 | 50823584 | - | MAD1L1 | chr7 | 2188873 | - |
intron-3CDS | ENST00000402497 | ENST00000406869 | GRB10 | chr7 | 50823584 | - | MAD1L1 | chr7 | 2188873 | - |
intron-3CDS | ENST00000402497 | ENST00000265854 | GRB10 | chr7 | 50823584 | - | MAD1L1 | chr7 | 2188873 | - |
intron-intron | ENST00000402497 | ENST00000486340 | GRB10 | chr7 | 50823584 | - | MAD1L1 | chr7 | 2188873 | - |
Frame-shift | ENST00000483819 | ENST00000402746 | GRB10 | chr7 | 50823584 | - | MAD1L1 | chr7 | 2188873 | - |
Frame-shift | ENST00000483819 | ENST00000399654 | GRB10 | chr7 | 50823584 | - | MAD1L1 | chr7 | 2188873 | - |
Frame-shift | ENST00000483819 | ENST00000406869 | GRB10 | chr7 | 50823584 | - | MAD1L1 | chr7 | 2188873 | - |
Frame-shift | ENST00000483819 | ENST00000265854 | GRB10 | chr7 | 50823584 | - | MAD1L1 | chr7 | 2188873 | - |
5CDS-intron | ENST00000483819 | ENST00000486340 | GRB10 | chr7 | 50823584 | - | MAD1L1 | chr7 | 2188873 | - |
ORFfinder result based on the fusion transcript sequence of in-frame fusion genes. |
Henst | Tenst | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand | Seq length (transcript) | BP loci (transcript) | Predicted start (transcript) | Predicted stop (transcript) | Seq length (amino acids) |
DeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated. |
Henst | Tenst | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand | No-coding score | Coding score |
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Fusion Genomic Features for GRB10-MAD1L1 |
FusionAI prediction of the potential fusion gene breakpoint based on the pre-mature RNA sequence context (+/- 5kb of individual partner genes, total 20kb length sequence). FusionAI is a fusion gene breakpoint classifier based on convolutional neural network by comparing the fusion positive and negative sequence context of ~ 20K fusion gene data. From here, we can have the relative potentency of the 20K genomic sequence how individual sequnce will be likely used as the gene fusion breakpoints. |
Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand | 1-p | p (fusion gene breakpoint) |
Distribution of 44 human genomic features loci across 20kb length fusion breakpoint regions. We integrated a total of 44 different types of human genomic feature loci information across five big categories including virus integration sites, repeats, structural variants, chromatin states, and gene expression regulation. More details are in help page. |
Distribution of 44 human genomic features loci across 20kb length fusion breakpoint regions that are ovelapped with the top 1% feature importance score regions. More details are in help page. |
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Fusion Protein Features for GRB10-MAD1L1 |
Go to FGviewer for the breakpoints of :-: - FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels. |
Main function of each fusion partner protein. (from UniProt) |
Hgene | Tgene |
GRB10 | MAD1L1 |
FUNCTION: Adapter protein which modulates coupling of a number of cell surface receptor kinases with specific signaling pathways. Binds to, and suppress signals from, activated receptors tyrosine kinases, including the insulin (INSR) and insulin-like growth factor (IGF1R) receptors. The inhibitory effect can be achieved by 2 mechanisms: interference with the signaling pathway and increased receptor degradation. Delays and reduces AKT1 phosphorylation in response to insulin stimulation. Blocks association between INSR and IRS1 and IRS2 and prevents insulin-stimulated IRS1 and IRS2 tyrosine phosphorylation. Recruits NEDD4 to IGF1R, leading to IGF1R ubiquitination, increased internalization and degradation by both the proteasomal and lysosomal pathways. May play a role in mediating insulin-stimulated ubiquitination of INSR, leading to proteasomal degradation. Negatively regulates Wnt signaling by interacting with LRP6 intracellular portion and interfering with the binding of AXIN1 to LRP6. Positive regulator of the KDR/VEGFR-2 signaling pathway. May inhibit NEDD4-mediated degradation of KDR/VEGFR-2. {ECO:0000269|PubMed:12493740, ECO:0000269|PubMed:15060076, ECO:0000269|PubMed:16434550, ECO:0000269|PubMed:17376403}. | FUNCTION: Component of the spindle-assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate (PubMed:10049595, PubMed:20133940, PubMed:29162720). Forms a heterotetrameric complex with the closed conformation form of MAD2L1 (C-MAD2) at unattached kinetochores during prometaphase, recruits an open conformation of MAD2L1 (O-MAD2) and promotes the conversion of O-MAD2 to C-MAD2, which ensures mitotic checkpoint signaling (PubMed:29162720). {ECO:0000269|PubMed:10049595, ECO:0000269|PubMed:20133940, ECO:0000269|PubMed:29162720}. |
Retention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at * Minus value of BPloci means that the break pointn is located before the CDS. |
- In-frame and retained protein feature among the 13 regional features. |
Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Protein feature | Protein feature note |
- In-frame and not-retained protein feature among the 13 regional features. |
Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Protein feature | Protein feature note |
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Fusion Gene Sequence for GRB10-MAD1L1 |
For in-frame fusion transcripts, we provide the fusion transcript sequences and fusion amino acid sequences. To have fusion amino acid sequence, we ran ORFfinder and chose the longest ORF among the all predicted ones. |
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Fusion Gene PPI Analysis for GRB10-MAD1L1 |
Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in |
Protein-protein interactors with each fusion partner protein in wild-type (BIOGRID-3.4.160) |
Hgene | Hgene's interactors | Tgene | Tgene's interactors |
- Retained PPIs in in-frame fusion. |
Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Still interaction with |
- Lost PPIs in in-frame fusion. |
Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Interaction lost with |
- Retained PPIs, but lost function due to frame-shift fusion. |
Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Interaction lost with |
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Related Drugs for GRB10-MAD1L1 |
Drugs targeting genes involved in this fusion gene. (DrugBank Version 5.1.8 2021-05-08) |
Partner | Gene | UniProtAcc | DrugBank ID | Drug name | Drug activity | Drug type | Drug status |
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Related Diseases for GRB10-MAD1L1 |
Diseases associated with fusion partners. (DisGeNet 4.0) |
Partner | Gene | Disease ID | Disease name | # pubmeds | Source |
Hgene | GRB10 | C0036341 | Schizophrenia | 1 | PSYGENET |
Tgene | MAD1L1 | C0036341 | Schizophrenia | 1 | PSYGENET |
Tgene | MAD1L1 | C0087031 | Juvenile-Onset Still Disease | 1 | CTD_human |
Tgene | MAD1L1 | C1336708 | Testicular Germ Cell Tumor | 1 | CTD_human |
Tgene | MAD1L1 | C2931456 | Prostate cancer, familial | 1 | CTD_human |
Tgene | MAD1L1 | C3495559 | Juvenile arthritis | 1 | CTD_human |
Tgene | MAD1L1 | C3714758 | Juvenile psoriatic arthritis | 1 | CTD_human |
Tgene | MAD1L1 | C4552091 | Polyarthritis, Juvenile, Rheumatoid Factor Negative | 1 | CTD_human |
Tgene | MAD1L1 | C4704862 | Polyarthritis, Juvenile, Rheumatoid Factor Positive | 1 | CTD_human |
Tgene | MAD1L1 | C4722327 | PROSTATE CANCER, HEREDITARY, 1 | 1 | CTD_human |