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 | Fusion Gene Summary |
| Fusion Gene ORF analysis |
| Fusion Genomic Features |
| Fusion Protein Features |
| Fusion Gene Sequence |
| Fusion Gene PPI analysis |
| Related Drugs |
| Related Diseases |
Fusion gene:GRK5-PDZD8 (FusionGDB2 ID:34760) |
Fusion Gene Summary for GRK5-PDZD8 |
 Fusion gene summary |
| Fusion gene information | Fusion gene name: GRK5-PDZD8 | Fusion gene ID: 34760 | Hgene | Tgene | Gene symbol | GRK5 | PDZD8 | Gene ID | 2869 | 118987 |
| Gene name | G protein-coupled receptor kinase 5 | PDZ domain containing 8 | |
| Synonyms | FP2025|GPRK5 | PDZK8 | |
| Cytomap | 10q26.11 | 10q25.3-q26.11 | |
| Type of gene | protein-coding | protein-coding | |
| Description | G protein-coupled receptor kinase 5g protein-coupled receptor kinase GRK5 | PDZ domain-containing protein 8sarcoma antigen NY-SAR-84/NY-SAR-104 | |
| Modification date | 20200313 | 20200313 | |
| UniProtAcc | P34947 | . | |
| Ensembl transtripts involved in fusion gene | ENST00000392870, ENST00000369108, ENST00000473264, | ENST00000334464, ENST00000482496, | |
| Fusion gene scores | * DoF score | 8 X 8 X 4=256 | 6 X 7 X 3=126 |
| # samples | 9 | 7 | |
| ** MAII score | log2(9/256*10)=-1.50814690367033 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0 | log2(7/126*10)=-0.84799690655495 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0 | |
| Context | PubMed: GRK5 [Title/Abstract] AND PDZD8 [Title/Abstract] AND fusion [Title/Abstract] | ||
| Most frequent breakpoint | GRK5(121156284)-PDZD8(119078485), # samples:2 PDZD8(119078382)-GRK5(121189886), # samples:1 | ||
| Anticipated loss of major functional domain due to fusion event. | PDZD8-GRK5 seems lost the major protein functional domain in Hgene partner, which is a essential gene due to the frame-shifted ORF. PDZD8-GRK5 seems lost the major protein functional domain in Tgene partner, which is a kinase due to the frame-shifted ORF. PDZD8-GRK5 seems lost the major protein functional domain in Tgene partner, which is a essential gene due to the frame-shifted ORF. PDZD8-GRK5 seems lost the major protein functional domain in Tgene partner, which is a IUPHAR drug target due to the frame-shifted ORF. GRK5-PDZD8 seems lost the major protein functional domain in Hgene partner, which is a kinase due to the frame-shifted ORF. GRK5-PDZD8 seems lost the major protein functional domain in Hgene partner, which is a essential gene due to the frame-shifted ORF. GRK5-PDZD8 seems lost the major protein functional domain in Hgene partner, which is a IUPHAR drug target due to the frame-shifted ORF. GRK5-PDZD8 seems lost the major protein functional domain in Tgene partner, which is a essential gene due to the frame-shifted ORF. | ||
| * DoF score (Degree of Frequency) = # partners X # break points X # cancer types ** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10) |
| Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez |
| Partner | Gene | GO ID | GO term | PubMed ID |
| Hgene | GRK5 | GO:0007217 | tachykinin receptor signaling pathway | 17986524 |
| Hgene | GRK5 | GO:0043066 | negative regulation of apoptotic process | 20124405 |
| Hgene | GRK5 | GO:0046777 | protein autophosphorylation | 14976207 |
| Tgene | PDZD8 | GO:0051560 | mitochondrial calcium ion homeostasis | 29097544 |
| Tgene | PDZD8 | GO:1990456 | mitochondrion-endoplasmic reticulum membrane tethering | 29097544 |
| Fusion gene breakpoints across GRK5 (5'-gene) * Click on the image to open the UCSC genome browser with custom track showing this image in a new window. |
| Fusion gene breakpoints across PDZD8 (3'-gene) * Click on the image to open the UCSC genome browser with custom track showing this image in a new window. |
| Fusion gene information from two resources (ChiTars 5.0 and ChimerDB 4.0) * All genome coordinats were lifted-over on hg19. * Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser. |
| Source | Disease | Sample | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand |
| ChimerDB4 | HNSC | TCGA-BA-4075-01A | GRK5 | chr10 | 121156284 | + | PDZD8 | chr10 | 119078485 | - |
| ChimerDB4 | HNSC | TCGA-BA-4075-01A | GRK5 | chr10 | 121156284 | - | PDZD8 | chr10 | 119078485 | - |
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Fusion Gene ORF analysis for GRK5-PDZD8 |
| Open reading frame (ORF) analsis of fusion genes based on Ensembl gene isoform structure. * Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser. |
| ORF | Henst | Tenst | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand |
| Frame-shift | ENST00000392870 | ENST00000334464 | GRK5 | chr10 | 121156284 | + | PDZD8 | chr10 | 119078485 | - |
| 5CDS-intron | ENST00000392870 | ENST00000482496 | GRK5 | chr10 | 121156284 | + | PDZD8 | chr10 | 119078485 | - |
| Frame-shift | ENST00000369108 | ENST00000334464 | GRK5 | chr10 | 121156284 | + | PDZD8 | chr10 | 119078485 | - |
| 5CDS-intron | ENST00000369108 | ENST00000482496 | GRK5 | chr10 | 121156284 | + | PDZD8 | chr10 | 119078485 | - |
| intron-3CDS | ENST00000473264 | ENST00000334464 | GRK5 | chr10 | 121156284 | + | PDZD8 | chr10 | 119078485 | - |
| intron-intron | ENST00000473264 | ENST00000482496 | GRK5 | chr10 | 121156284 | + | PDZD8 | chr10 | 119078485 | - |
| ORFfinder result based on the fusion transcript sequence of in-frame fusion genes. |
| Henst | Tenst | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand | Seq length (transcript) | BP loci (transcript) | Predicted start (transcript) | Predicted stop (transcript) | Seq length (amino acids) |
| DeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated. |
| Henst | Tenst | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand | No-coding score | Coding score |
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Fusion Genomic Features for GRK5-PDZD8 |
| FusionAI prediction of the potential fusion gene breakpoint based on the pre-mature RNA sequence context (+/- 5kb of individual partner genes, total 20kb length sequence). FusionAI is a fusion gene breakpoint classifier based on convolutional neural network by comparing the fusion positive and negative sequence context of ~ 20K fusion gene data. From here, we can have the relative potentency of the 20K genomic sequence how individual sequnce will be likely used as the gene fusion breakpoints. |
| Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand | 1-p | p (fusion gene breakpoint) |
| Distribution of 44 human genomic features loci across 20kb length fusion breakpoint regions. We integrated a total of 44 different types of human genomic feature loci information across five big categories including virus integration sites, repeats, structural variants, chromatin states, and gene expression regulation. More details are in help page. |
| Distribution of 44 human genomic features loci across 20kb length fusion breakpoint regions that are ovelapped with the top 1% feature importance score regions. More details are in help page. |
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Fusion Protein Features for GRK5-PDZD8 |
| Go to FGviewer for the breakpoints of :-: - FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels. |
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| Main function of each fusion partner protein. (from UniProt) |
| Hgene | Tgene |
| GRK5 | . |
| FUNCTION: Serine/threonine kinase that phosphorylates preferentially the activated forms of a variety of G-protein-coupled receptors (GPCRs). Such receptor phosphorylation initiates beta-arrestin-mediated receptor desensitization, internalization, and signaling events leading to their down-regulation. Phosphorylates a variety of GPCRs, including adrenergic receptors, muscarinic acetylcholine receptors (more specifically Gi-coupled M2/M4 subtypes), dopamine receptors and opioid receptors. In addition to GPCRs, also phosphorylates various substrates: Hsc70-interacting protein/ST13, TP53/p53, HDAC5, and arrestin-1/ARRB1. Phosphorylation of ARRB1 by GRK5 inhibits G-protein independent MAPK1/MAPK3 signaling downstream of 5HT4-receptors. Phosphorylation of HDAC5, a repressor of myocyte enhancer factor 2 (MEF2) leading to nuclear export of HDAC5 and allowing MEF2-mediated transcription. Phosphorylation of TP53/p53, a crucial tumor suppressor, inhibits TP53/p53-mediated apoptosis. Phosphorylation of ST13 regulates internalization of the chemokine receptor. Phosphorylates rhodopsin (RHO) (in vitro) and a non G-protein-coupled receptor, LRP6 during Wnt signaling (in vitro). {ECO:0000269|PubMed:19661922, ECO:0000269|PubMed:19801552, ECO:0000269|PubMed:20038610, ECO:0000269|PubMed:20124405, ECO:0000269|PubMed:21728385}. | FUNCTION: Might normally function as a transcriptional repressor. EWS-fusion-proteins (EFPS) may play a role in the tumorigenic process. They may disturb gene expression by mimicking, or interfering with the normal function of CTD-POLII within the transcription initiation complex. They may also contribute to an aberrant activation of the fusion protein target genes. |
| Retention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at * Minus value of BPloci means that the break pointn is located before the CDS. |
| - In-frame and retained protein feature among the 13 regional features. |
| Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Protein feature | Protein feature note |
| - In-frame and not-retained protein feature among the 13 regional features. |
| Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Protein feature | Protein feature note |
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Fusion Gene Sequence for GRK5-PDZD8 |
| For in-frame fusion transcripts, we provide the fusion transcript sequences and fusion amino acid sequences. To have fusion amino acid sequence, we ran ORFfinder and chose the longest ORF among the all predicted ones. |
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Fusion Gene PPI Analysis for GRK5-PDZD8 |
| Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in |
| Protein-protein interactors with each fusion partner protein in wild-type (BIOGRID-3.4.160) |
| Hgene | Hgene's interactors | Tgene | Tgene's interactors |
| - Retained PPIs in in-frame fusion. |
| Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Still interaction with |
| - Lost PPIs in in-frame fusion. |
| Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Interaction lost with |
| - Retained PPIs, but lost function due to frame-shift fusion. |
| Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Interaction lost with |
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Related Drugs for GRK5-PDZD8 |
| Drugs targeting genes involved in this fusion gene. (DrugBank Version 5.1.8 2021-05-08) |
| Partner | Gene | UniProtAcc | DrugBank ID | Drug name | Drug activity | Drug type | Drug status |
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Related Diseases for GRK5-PDZD8 |
| Diseases associated with fusion partners. (DisGeNet 4.0) |
| Partner | Gene | Disease ID | Disease name | # pubmeds | Source |
| Hgene | GRK5 | C0007222 | Cardiovascular Diseases | 1 | CTD_human |
| Hgene | GRK5 | C0023895 | Liver diseases | 1 | CTD_human |
| Hgene | GRK5 | C0086565 | Liver Dysfunction | 1 | CTD_human |
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