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	Fusion Gene Summary
	Fusion Gene ORF analysis
	Fusion Genomic Features
	Fusion Protein Features
	Fusion Gene Sequence
	Fusion Gene PPI analysis
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Fusion gene:HCFC1-EPHA5 (FusionGDB2 ID:35596)

Fusion Gene Summary for HCFC1-EPHA5

Fusion gene summary

Fusion gene information	Fusion gene name: HCFC1-EPHA5
	Fusion gene ID: 35596
		Hgene	Tgene
	Gene symbol	HCFC1	EPHA5
	Gene ID	3054	2044
	Gene name	host cell factor C1	EPH receptor A5
	Synonyms	CFF\|HCF\|HCF-1\|HCF1\|HFC1\|MRX3\|PPP1R89\|VCAF	CEK7\|EHK-1\|EHK1\|EK7\|HEK7\|TYRO4
	Cytomap	Xq28	4q13.1-q13.2
	Type of gene	protein-coding	protein-coding
	Description	host cell factor 1VP16-accessory proteinprotein phosphatase 1, regulatory subunit 89	ephrin type-A receptor 5EPH homology kinase 1EPH-like kinase 7brain-specific kinaseepididymis secretory sperm binding proteinreceptor protein-tyrosine kinase HEK7tyrosine-protein kinase receptor EHK-1
	Modification date	20200313	20200313
	UniProtAcc	P51610	P54756
	Ensembl transtripts involved in fusion gene	ENST00000310441, ENST00000369984, ENST00000354233, ENST00000461098,	ENST00000273854, ENST00000432638, ENST00000354839, ENST00000511294,
Fusion gene scores	* DoF score	16 X 17 X 8=2176	6 X 5 X 3=90
	# samples	25	5
	** MAII score	log2(25/2176*10)=-3.12167855658825 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0	log2(5/90*10)=-0.84799690655495 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0
Context	PubMed: HCFC1 [Title/Abstract] AND EPHA5 [Title/Abstract] AND fusion [Title/Abstract]
Most frequent breakpoint	HCFC1(153236099)-EPHA5(66189937), # samples:3
Anticipated loss of major functional domain due to fusion event.	HCFC1-EPHA5 seems lost the major protein functional domain in Hgene partner, which is a essential gene due to the frame-shifted ORF. HCFC1-EPHA5 seems lost the major protein functional domain in Hgene partner, which is a epigenetic factor due to the frame-shifted ORF. HCFC1-EPHA5 seems lost the major protein functional domain in Tgene partner, which is a kinase due to the frame-shifted ORF. HCFC1-EPHA5 seems lost the major protein functional domain in Tgene partner, which is a IUPHAR drug target due to the frame-shifted ORF.

* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez

Partner	Gene	GO ID	GO term	PubMed ID
Hgene	HCFC1	GO:0006355	regulation of transcription, DNA-templated	12670868
Hgene	HCFC1	GO:0010628	positive regulation of gene expression	21285374
Hgene	HCFC1	GO:0043254	regulation of protein complex assembly	10675337
Hgene	HCFC1	GO:0043981	histone H4-K5 acetylation	20018852
Hgene	HCFC1	GO:0043982	histone H4-K8 acetylation	20018852
Hgene	HCFC1	GO:0043984	histone H4-K16 acetylation	20018852
Hgene	HCFC1	GO:0050821	protein stabilization	21285374

Fusion gene breakpoints across HCFC1 (5'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

Fusion gene breakpoints across EPHA5 (3'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

Fusion gene information from two resources (ChiTars 5.0 and ChimerDB 4.0)
* All genome coordinats were lifted-over on hg19.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.

Source	Disease	Sample	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand
ChimerDB4	LUSC	TCGA-LA-A446-01A	HCFC1	chrX	153236099	-	EPHA5	chr4	66189937	-
ChimerDB4	LUSC	TCGA-LA-A446	HCFC1	chrX	153236099	-	EPHA5	chr4	66189937	-
ChimerDB4	LUSC	TCGA-LA-A446-01A	HCFC1	chrX	153236099	-	EPHA5	chr4	66189937	-

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Fusion Gene ORF analysis for HCFC1-EPHA5

Open reading frame (ORF) analsis of fusion genes based on Ensembl gene isoform structure.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.

ORF	Henst	Tenst	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand
In-frame	ENST00000310441	ENST00000273854	HCFC1	chrX	153236099	-	EPHA5	chr4	66189937	-
Frame-shift	ENST00000310441	ENST00000432638	HCFC1	chrX	153236099	-	EPHA5	chr4	66189937	-
In-frame	ENST00000310441	ENST00000354839	HCFC1	chrX	153236099	-	EPHA5	chr4	66189937	-
5CDS-intron	ENST00000310441	ENST00000511294	HCFC1	chrX	153236099	-	EPHA5	chr4	66189937	-
In-frame	ENST00000369984	ENST00000273854	HCFC1	chrX	153236099	-	EPHA5	chr4	66189937	-
Frame-shift	ENST00000369984	ENST00000432638	HCFC1	chrX	153236099	-	EPHA5	chr4	66189937	-
In-frame	ENST00000369984	ENST00000354839	HCFC1	chrX	153236099	-	EPHA5	chr4	66189937	-
5CDS-intron	ENST00000369984	ENST00000511294	HCFC1	chrX	153236099	-	EPHA5	chr4	66189937	-
In-frame	ENST00000354233	ENST00000273854	HCFC1	chrX	153236099	-	EPHA5	chr4	66189937	-
Frame-shift	ENST00000354233	ENST00000432638	HCFC1	chrX	153236099	-	EPHA5	chr4	66189937	-
In-frame	ENST00000354233	ENST00000354839	HCFC1	chrX	153236099	-	EPHA5	chr4	66189937	-
5CDS-intron	ENST00000354233	ENST00000511294	HCFC1	chrX	153236099	-	EPHA5	chr4	66189937	-
intron-3CDS	ENST00000461098	ENST00000273854	HCFC1	chrX	153236099	-	EPHA5	chr4	66189937	-
intron-3CDS	ENST00000461098	ENST00000432638	HCFC1	chrX	153236099	-	EPHA5	chr4	66189937	-
intron-3CDS	ENST00000461098	ENST00000354839	HCFC1	chrX	153236099	-	EPHA5	chr4	66189937	-
intron-intron	ENST00000461098	ENST00000511294	HCFC1	chrX	153236099	-	EPHA5	chr4	66189937	-

ORFfinder result based on the fusion transcript sequence of in-frame fusion genes.

Henst	Tenst	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	Seq length (transcript)	BP loci (transcript)	Predicted start (transcript)	Predicted stop (transcript)	Seq length (amino acids)
ENST00000310441	HCFC1	chrX	153236099	-	ENST00000273854	EPHA5	chr4	66189937	-	5817	1160	1014	406	202
ENST00000310441	HCFC1	chrX	153236099	-	ENST00000354839	EPHA5	chr4	66189937	-	1452	1160	1014	406	202
ENST00000369984	HCFC1	chrX	153236099	-	ENST00000273854	EPHA5	chr4	66189937	-	5194	537	648	259	129
ENST00000369984	HCFC1	chrX	153236099	-	ENST00000354839	EPHA5	chr4	66189937	-	829	537	648	259	129
ENST00000354233	HCFC1	chrX	153236099	-	ENST00000273854	EPHA5	chr4	66189937	-	5378	721	832	443	129
ENST00000354233	HCFC1	chrX	153236099	-	ENST00000354839	EPHA5	chr4	66189937	-	1013	721	832	443	129

DeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.

Henst	Tenst	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	No-coding score	Coding score
ENST00000310441	ENST00000273854	HCFC1	chrX	153236099	-	EPHA5	chr4	66189937	-	0.79326963	0.20673038
ENST00000310441	ENST00000354839	HCFC1	chrX	153236099	-	EPHA5	chr4	66189937	-	0.44918746	0.5508126
ENST00000369984	ENST00000273854	HCFC1	chrX	153236099	-	EPHA5	chr4	66189937	-	0.8026404	0.1973596
ENST00000369984	ENST00000354839	HCFC1	chrX	153236099	-	EPHA5	chr4	66189937	-	0.46355402	0.536446
ENST00000354233	ENST00000273854	HCFC1	chrX	153236099	-	EPHA5	chr4	66189937	-	0.79764384	0.20235611
ENST00000354233	ENST00000354839	HCFC1	chrX	153236099	-	EPHA5	chr4	66189937	-	0.43971318	0.5602869

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Fusion Genomic Features for HCFC1-EPHA5

FusionAI prediction of the potential fusion gene breakpoint based on the pre-mature RNA sequence context (+/- 5kb of individual partner genes, total 20kb length sequence). FusionAI is a fusion gene breakpoint classifier based on convolutional neural network by comparing the fusion positive and negative sequence context of ~ 20K fusion gene data. From here, we can have the relative potentency of the 20K genomic sequence how individual sequnce will be likely used as the gene fusion breakpoints.

Hgene

Hchr

Hbp

Hstrand

Tgene

Tchr

Tbp

Tstrand

1-p

p (fusion gene breakpoint)

Distribution of 44 human genomic features loci across 20kb length fusion breakpoint regions. We integrated a total of 44 different types of human genomic feature loci information across five big categories including virus integration sites, repeats, structural variants, chromatin states, and gene expression regulation. More details are in help page.

Distribution of 44 human genomic features loci across 20kb length fusion breakpoint regions that are ovelapped with the top 1% feature importance score regions. More details are in help page.

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Fusion Protein Features for HCFC1-EPHA5

Go to
FGviewer for the breakpoints of chrX:153236099-chr4:66189937
.
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.

Main function of each fusion partner protein. (from UniProt)

Hgene	Tgene
HCFC1 P51610	EPHA5 P54756
FUNCTION: Involved in control of the cell cycle (PubMed:10629049, PubMed:10779346, PubMed:15190068, PubMed:16624878, PubMed:23629655). Also antagonizes transactivation by ZBTB17 and GABP2; represses ZBTB17 activation of the p15(INK4b) promoter and inhibits its ability to recruit p300 (PubMed:10675337, PubMed:12244100). Coactivator for EGR2 and GABP2 (PubMed:12244100, PubMed:14532282). Tethers the chromatin modifying Set1/Ash2 histone H3 'Lys-4' methyltransferase (H3K4me) and Sin3 histone deacetylase (HDAC) complexes (involved in the activation and repression of transcription, respectively) together (PubMed:12670868). Component of a THAP1/THAP3-HCFC1-OGT complex that is required for the regulation of the transcriptional activity of RRM1 (PubMed:20200153). As part of the NSL complex it may be involved in acetylation of nucleosomal histone H4 on several lysine residues (PubMed:20018852). Recruits KMT2E/MLL5 to E2F1 responsive promoters promoting transcriptional activation and thereby facilitates G1 to S phase transition (PubMed:23629655). {ECO:0000269\|PubMed:10629049, ECO:0000269\|PubMed:10675337, ECO:0000269\|PubMed:10779346, ECO:0000269\|PubMed:12244100, ECO:0000269\|PubMed:12670868, ECO:0000269\|PubMed:14532282, ECO:0000269\|PubMed:15190068, ECO:0000269\|PubMed:16624878, ECO:0000269\|PubMed:20018852, ECO:0000269\|PubMed:20200153, ECO:0000269\|PubMed:23629655}.; FUNCTION: (Microbial infection) In case of human herpes simplex virus (HSV) infection, HCFC1 forms a multiprotein-DNA complex with the viral transactivator protein VP16 and POU2F1 thereby enabling the transcription of the viral immediate early genes. {ECO:0000269\|PubMed:10629049, ECO:0000269\|PubMed:17578910}.	FUNCTION: Receptor tyrosine kinase which binds promiscuously GPI-anchored ephrin-A family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Among GPI-anchored ephrin-A ligands, EFNA5 most probably constitutes the cognate/functional ligand for EPHA5. Functions as an axon guidance molecule during development and may be involved in the development of the retinotectal, entorhino-hippocampal and hippocamposeptal pathways. Together with EFNA5 plays also a role in synaptic plasticity in adult brain through regulation of synaptogenesis. In addition to its function in the nervous system, the interaction of EPHA5 with EFNA5 mediates communication between pancreatic islet cells to regulate glucose-stimulated insulin secretion (By similarity). {ECO:0000250}.

Retention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at
download page

* Minus value of BPloci means that the break pointn is located before the CDS.

- In-frame and retained protein feature among the 13 regional features.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Protein feature

Protein feature note

Tgene

EPHA5

chrX:153236099

chr4:66189937

ENST00000273854

1035_1037

1002.6666666666666

1038.0

Motif

PDZ-binding

Tgene

EPHA5

chrX:153236099

chr4:66189937

ENST00000354839

1035_1037

980.6666666666666

1016.0

Motif

PDZ-binding

- In-frame and not-retained protein feature among the 13 regional features.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Protein feature

Protein feature note

Hgene

HCFC1

chrX:153236099

chr4:66189937

ENST00000310441

1797_1888

64.33333333333333

2036.0

Domain

Fibronectin type-III 2

Hgene

HCFC1

chrX:153236099

chr4:66189937

ENST00000310441

1890_2006

64.33333333333333

2036.0

Domain

Fibronectin type-III 3

Hgene

HCFC1

chrX:153236099

chr4:66189937

ENST00000310441

366_466

64.33333333333333

2036.0

Domain

Fibronectin type-III 1

Hgene

HCFC1

chrX:153236099

chr4:66189937

ENST00000354233

1797_1888

64.33333333333333

1967.0

Domain

Fibronectin type-III 2

Hgene

HCFC1

chrX:153236099

chr4:66189937

ENST00000354233

1890_2006

64.33333333333333

1967.0

Domain

Fibronectin type-III 3

Hgene

HCFC1

chrX:153236099

chr4:66189937

ENST00000354233

366_466

64.33333333333333

1967.0

Domain

Fibronectin type-III 1

Hgene

HCFC1

chrX:153236099

chr4:66189937

ENST00000310441

1010_1035

64.33333333333333

2036.0

Repeat

Note=HCF repeat 1

Hgene

HCFC1

chrX:153236099

chr4:66189937

ENST00000310441

1072_1097

64.33333333333333

2036.0

Repeat

Note=HCF repeat 2

Hgene

HCFC1

chrX:153236099

chr4:66189937

ENST00000310441

1101_1126

64.33333333333333

2036.0

Repeat

Note=HCF repeat 3

Hgene

HCFC1

chrX:153236099

chr4:66189937

ENST00000310441

1158_1183

64.33333333333333

2036.0

Repeat

Note=HCF repeat 4%3B degenerate

Hgene

HCFC1

chrX:153236099

chr4:66189937

ENST00000310441

1286_1311

64.33333333333333

2036.0

Repeat

Note=HCF repeat 5

Hgene

HCFC1

chrX:153236099

chr4:66189937

ENST00000310441

1314_1339

64.33333333333333

2036.0

Repeat

Note=HCF repeat 6

Hgene

HCFC1

chrX:153236099

chr4:66189937

ENST00000310441

1349_1374

64.33333333333333

2036.0

Repeat

Note=HCF repeat 7%3B degenerate

Hgene

HCFC1

chrX:153236099

chr4:66189937

ENST00000310441

1414_1439

64.33333333333333

2036.0

Repeat

Note=HCF repeat 8

Hgene

HCFC1

chrX:153236099

chr4:66189937

ENST00000310441

148_194

64.33333333333333

2036.0

Repeat

Note=Kelch 3

Hgene

HCFC1

chrX:153236099

chr4:66189937

ENST00000310441

217_265

64.33333333333333

2036.0

Repeat

Note=Kelch 4

Hgene

HCFC1

chrX:153236099

chr4:66189937

ENST00000310441

266_313

64.33333333333333

2036.0

Repeat

Note=Kelch 5

Hgene

HCFC1

chrX:153236099

chr4:66189937

ENST00000310441

44_89

64.33333333333333

2036.0

Repeat

Note=Kelch 1

Hgene

HCFC1

chrX:153236099

chr4:66189937

ENST00000310441

93_140

64.33333333333333

2036.0

Repeat

Note=Kelch 2

Hgene

HCFC1

chrX:153236099

chr4:66189937

ENST00000354233

1010_1035

64.33333333333333

1967.0

Repeat

Note=HCF repeat 1

Hgene

HCFC1

chrX:153236099

chr4:66189937

ENST00000354233

1072_1097

64.33333333333333

1967.0

Repeat

Note=HCF repeat 2

Hgene

HCFC1

chrX:153236099

chr4:66189937

ENST00000354233

1101_1126

64.33333333333333

1967.0

Repeat

Note=HCF repeat 3

Hgene

HCFC1

chrX:153236099

chr4:66189937

ENST00000354233

1158_1183

64.33333333333333

1967.0

Repeat

Note=HCF repeat 4%3B degenerate

Hgene

HCFC1

chrX:153236099

chr4:66189937

ENST00000354233

1286_1311

64.33333333333333

1967.0

Repeat

Note=HCF repeat 5

Hgene

HCFC1

chrX:153236099

chr4:66189937

ENST00000354233

1314_1339

64.33333333333333

1967.0

Repeat

Note=HCF repeat 6

Hgene

HCFC1

chrX:153236099

chr4:66189937

ENST00000354233

1349_1374

64.33333333333333

1967.0

Repeat

Note=HCF repeat 7%3B degenerate

Hgene

HCFC1

chrX:153236099

chr4:66189937

ENST00000354233

1414_1439

64.33333333333333

1967.0

Repeat

Note=HCF repeat 8

Hgene

HCFC1

chrX:153236099

chr4:66189937

ENST00000354233

148_194

64.33333333333333

1967.0

Repeat

Note=Kelch 3

Hgene

HCFC1

chrX:153236099

chr4:66189937

ENST00000354233

217_265

64.33333333333333

1967.0

Repeat

Note=Kelch 4

Hgene

HCFC1

chrX:153236099

chr4:66189937

ENST00000354233

266_313

64.33333333333333

1967.0

Repeat

Note=Kelch 5

Hgene

HCFC1

chrX:153236099

chr4:66189937

ENST00000354233

44_89

64.33333333333333

1967.0

Repeat

Note=Kelch 1

Hgene

HCFC1

chrX:153236099

chr4:66189937

ENST00000354233

93_140

64.33333333333333

1967.0

Repeat

Note=Kelch 2

Tgene

EPHA5

chrX:153236099

chr4:66189937

ENST00000273854

220_354

1002.6666666666666

1038.0

Compositional bias

Note=Cys-rich

Tgene

EPHA5

chrX:153236099

chr4:66189937

ENST00000354839

220_354

980.6666666666666

1016.0

Compositional bias

Note=Cys-rich

Tgene

EPHA5

chrX:153236099

chr4:66189937

ENST00000273854

357_467

1002.6666666666666

1038.0

Domain

Fibronectin type-III 1

Tgene

EPHA5

chrX:153236099

chr4:66189937

ENST00000273854

468_562

1002.6666666666666

1038.0

Domain

Fibronectin type-III 2

Tgene

EPHA5

chrX:153236099

chr4:66189937

ENST00000273854

60_238

1002.6666666666666

1038.0

Domain

Eph LBD

Tgene

EPHA5

chrX:153236099

chr4:66189937

ENST00000273854

675_936

1002.6666666666666

1038.0

Domain

Protein kinase

Tgene

EPHA5

chrX:153236099

chr4:66189937

ENST00000273854

965_1029

1002.6666666666666

1038.0

Domain

SAM

Tgene

EPHA5

chrX:153236099

chr4:66189937

ENST00000354839

357_467

980.6666666666666

1016.0

Domain

Fibronectin type-III 1

Tgene

EPHA5

chrX:153236099

chr4:66189937

ENST00000354839

468_562

980.6666666666666

1016.0

Domain

Fibronectin type-III 2

Tgene

EPHA5

chrX:153236099

chr4:66189937

ENST00000354839

60_238

980.6666666666666

1016.0

Domain

Eph LBD

Tgene

EPHA5

chrX:153236099

chr4:66189937

ENST00000354839

675_936

980.6666666666666

1016.0

Domain

Protein kinase

Tgene

EPHA5

chrX:153236099

chr4:66189937

ENST00000354839

965_1029

980.6666666666666

1016.0

Domain

SAM

Tgene

EPHA5

chrX:153236099

chr4:66189937

ENST00000273854

681_689

1002.6666666666666

1038.0

Nucleotide binding

ATP

Tgene

EPHA5

chrX:153236099

chr4:66189937

ENST00000354839

681_689

980.6666666666666

1016.0

Nucleotide binding

ATP

Tgene

EPHA5

chrX:153236099

chr4:66189937

ENST00000273854

25_573

1002.6666666666666

1038.0

Topological domain

Extracellular

Tgene

EPHA5

chrX:153236099

chr4:66189937

ENST00000273854

595_1037

1002.6666666666666

1038.0

Topological domain

Cytoplasmic

Tgene

EPHA5

chrX:153236099

chr4:66189937

ENST00000354839

25_573

980.6666666666666

1016.0

Topological domain

Extracellular

Tgene

EPHA5

chrX:153236099

chr4:66189937

ENST00000354839

595_1037

980.6666666666666

1016.0

Topological domain

Cytoplasmic

Tgene

EPHA5

chrX:153236099

chr4:66189937

ENST00000273854

574_594

1002.6666666666666

1038.0

Transmembrane

Helical

Tgene

EPHA5

chrX:153236099

chr4:66189937

ENST00000354839

574_594

980.6666666666666

1016.0

Transmembrane

Helical

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Fusion Gene Sequence for HCFC1-EPHA5

For in-frame fusion transcripts, we provide the fusion transcript sequences and fusion amino acid sequences. To have fusion amino acid sequence, we ran ORFfinder and chose the longest ORF among the all predicted ones.

>In-frame_ENST00000310441_ENST00000273854_TCGA-LA-A446-01A_HCFC1_chrX_153236099_-_EPHA5_chr4_66189937_length(transcript)=5817nt_BP=1160nt
CGGCTCCCAGGGAGGAGGCATGAGCGCCCTGCCGTTACCGCTTCTTCTGCCGTACAGTTGCCGCTTCGGGGCCTAACTCTAGCTCTTTGA
AGCGGCTCCTGTGGGAAGGAGTCATAAGGCGGGAAGTGAGGATCGGACACCGCCTTGAGTTTGGTGTGCAGTGGAAGAACTGACCTAGGA
GGAAGAGAATAGGGGGGAACGGGGAGCTAGTCTCAAGATGGCGGCTCCCACGGCGGCAGGCGCAGCCTGAGCAGCAGAGGCGGACTAAGG
GGAGAGGCGCGAGACTTCACAAGCCGTACGGCGCGAGCCCCCAAACGGCCCTCGAGAGCTCGAGCTGGTTCCACCGGCCTCCGGCCCGCC
TCCATTCGTTAGGTGTGTCGAGGCTGTTTCGGGAGCGGCGGCTGCACTAAGGCGCTGGGCGGGGAAGCGGGACGGTCTCAAGATGGCGGC
TCCCACAATTGTGGTCTGAGCGCCGGCGGGGCTGCGACGACCGCGGCGCTTGTGGCTCCTTTCCACCCGCCCCGGAAGCCGGCCAGTGCT
GGGGACTTGGGGGTGTGGGAACCGGGCCGGGGCTCCGCCATTTCCGGCGGGGGAGGGCTACGACTGAGGAAGGGAGGAGAGAGAGGCGGC
TCAAGATGGCGGCTCCCAGGGCCTCCCGCCCGAGCTTGTAAGCGGGAGCGCCCGGACAAGTAGTCGGGGCGACGGGACTCAGCGGCCTCC
AGCTTCTTGAGCCTAGGCGCTCGACAGTTTCGGGCGGCTCTTGCGGAGACGGGGTGAGCGAGAAGAAAGGGAAGAGCCAAAGGGAAGGAG
GGCAGTTAAGATGGCGGCCTCCATGGAGTCGTCTACCGCTGTGTGAGAAACCGCTTCTCCGTGAGAGCTGCCTTAGACGAAAGGGGGTGT
GTGAAAGGAATTGAGGGGCTCCCTTCCCGCTTGTTGACTTCTCCCCACCGCACCCTTTCCCGGAACTATGGCTTCGGCCGTGTCGCCCGC
CAACTTGCCAGCGGTGCTTCTGCAGCCCCGCTGGAAGCGAGTGGTGGGCTGGTCGGGTCCGGTGCCACGGCCCCGCCACGGCCACCGCGC
CGTGGCCATCAAGGAGCTCATCGTGGTGTTTGGCGGCGGCAACGAGGGAATAGTGGACGAACTGCACGTGTACAACACGGGGATTTGAGA
CGGCTTGGAGTGACTCTTGTCGGTCACCAGAAGAAGATCATGAACAGCCTTCAAGAAATGAAGGTGCAGCTGGTAAACGGAATGGTGCCA
TTGTAACTTCATGTAAATGTCGCTTCTTCAAGTGAATGATTCTGCACTTTGTAAACAGCACTGAGATTTATTTTAACAAAAAAAGGGGGA
AAAGGGAAAACAGTGATTTCTAAACCTTAGAAAACATTTGCCTCAGCCACAGAATTTGTAATCATGGTTTTACTGAAGTATCCAGTTCTT
AGTCCTTAGTCTTCATTTTTCATGAAGCAAACATATCTTGCATTAAAAGGGACATGAAGTTAGACATCATCTTAAGTTACAACAACAGAA
TCCTTCCCACTACTTCTACAAAATTTTGTACATGAAATATATAATTATATAGCACTTTTATAGACTGAATTAAGGCAACCCCTTTCAAAA
CTTCCAGGGATCTACTTGAAAGGAAATGTTTTATAGCCATTTGTGAGCTAACAAAAGCTACAGTTTACTGAAGTTTACTTCAAGTCTTAA
TTGTCTACAAAAGTGTATTGAAGAGCAATATGATTAGATTATTTCTTAATAGATATCTTCTTTTGTAATTTTAAAATGCTGTTACACAGC
GTTAAGTTATAGAAACTAGTGTGTAAACATGTTGCTTGATCAAGAAAAAGTACAATACAGGGTGTATATTTATTTTTTGTGTTATAAAGT
TTACTTTTAGTTGCTCTTCTAGAGATTATTAGGTAATAAATGTGTATATACTGTATAATTTGCAATATACCCAGGAACTGATTTAAGATG
GAATTGTGTGTGTGTTTGCTTGCACATGTGTGTGTTACCATTCTGCTTGCATTTCTAATAGTGTTTCAATTTTAGCAACATATAGGAGCA
AGTGTTCCAGAATGTAATATGAATAGGAGAAATAGGGAAGCAGTAAAACAAAATTTAACACAAGCTTGTGTCTCTTTTCCTCTCATGTGT
CCAAAAGCTAATCTCTTTATTCACTAAAAGGAAATGTGTATAAGACTAAATCCCCTTTGGCTTTTTAAAAACATTTTGTGATATCAGTGA
CAATGCAGTTCTTCTAGCCATTAATCTTGTACCCCTGTAAGAAATTTCACCTTTCTGAGTCCTGAAAAGTATCTTGTCAAGCAAAGTTGA
CACCGAAGGGCACATTTTCAGCAGGATGTAGAAGGATTTAACTGTGCAGGCTTCTGTTAATGTTGTTAAATCCAGGCACATAGCACGAAG
CATCACCCTTAAGTGTTAATCCGTTGTAACCATTCCCATTTTGACTCAGTTCTAGAAATTTTGACTCTAAGGCAGCAATGGAATTATGAC
AAATATATATATATACACATACACACACACAAATATATATATATGCTTATTTCCCTTCAGAATTTTATTTCAATAATTGATAAGTTTTAT
TTTTAATGGATGTTTATTCATTTGTTAATTTCAGTTTGTATTCAGTTACATGGCTTTGAGTTTATTTTATTGAACCAATCCAAGGTATTA
TGTAATTAGGCTTATTAAGGAATATAACATATCTTCTATGTATGCTCTATATACCACACATGATGAAGACTTATATTTGTGATACAAATT
ATGCAGTGTATTAGAAATTGTCCAAAACTGAGCTCCTGAGATAACCAACATTTTTGTATTATTACACTTTATAAAAATTATGGAATGTCA
TGTGATTAGTCAAAATCAGAGCATTTAAATAAAATACAGCCTACTCATGATTATCAATGTTAATAGTACAGTAGATCAGAGAAGCCTGGA
TTAATGAAATTTATCAGAAATATAGCCACGTTATATTAATGGTATTCTTTTTTCCAAGCACTTGTTTTATTTGAATATTAGCACATATAA
GAATAAACTTTTGGGAAATAACTACTTCCTATTCTGCCCACCCTTTACTTAAGCAGTAAAGTACCTCTTTTCTAAAGAACCAGGAAACAT
CAAAATAACCACAACAAAAATCTAAACAGTTTTCAAATAATACATTCAGTTCTCATAATAATAAGGGCTATATACCATGGGTCCACACAA
ACTGTGCTGCAAACATATAATCTATTAGTTTAGTAAAATTTCCAGAGATGTGAATTTAATATTTTCTTGATAAAATCATAAAAAAGCATC
ACCATTATTAAAGATGCATTTGTTCATTTCAATAAACAGAAATTAATGAAACAAATTACTTTTGTACAAAATAAATGATGATTATGTGGC
ACTTTATTGAATTTTATAGAAATATTCCAATCTAAGTGATAAAGTAACAACTGAGAAACTTCAGAATGAAAGCCTTATTGATTCAAAAGG
AAAATTACCATATGCTTTAGGGCTGATGAACTTGCCACAATTGCTTAGACATAACAAAGATATTTTCTACATTGTTTTCCAACTTTATAT
GCTGAAAGATAATTAGGAGGTCCGGGTGGAGTGTATCTAAATGACTAGAACTTTAAGTTGCAATATAGATTTTCTCTTTTTAACAAAGGA
AGAATATAAATTAATTTGGATCAAATTTATTTGCCTTCTTTGCAATCTTGGTGATCATTTTGGAAAGTAAATTGAAAGGAAAGTTAAATA
GCCACATAGTTTTCTTTTGCATCTCAATTTGGTTGAGAATTTCTAAGGAAGGTTAATATGACTTTAGAATGAATAAAGAGACTGTCAAAC
AACAAGTACTCTCCCTAAAAAAGAGGAAAGAATGCCTTCCTAAAATATTTGTTTCCTATTGTATATGTCACCAATTGGAAAATGCCAATC
TCATCTGCAAGTGTGAGAAATGGAACAAGGAGAGGAGCCAACTCTAGTAAAATGTAGTGAGGCCATAATCCAAGGCAAAGTTTAACAAAT
CTTGATTGTGTAATTTCTTATTCTGTTGATTCATTTTGTCACCCACCAGACTACCAAAAATAAAGCACAGACATGACAATTTTAGTATAT
ACAACAGATTTGGCAAATAATACATAATCTTATTGTAATTGCCAGTAGCAATATCTTAAGGGGCATGAGTCTTTCACAGTATTGGACTTT
GAAAAATTGGTACAGGGAGTAATTTTCAACAGACTGATACTTGAAGTCACTCAATTACTACTTTGACCTATCATAATTTGTTTCTAATCT
TGTCATTTGCTCATATATACTATTAATATAACCAGTTGCTCTTTTCATAATGTACTATTTCTATGTAAAGATGTAAAGATTTTGATTTTC
CTATGTTTTAAAATTGTAATGTGCATGATCTTTTATTTCTTGTGTTTCAATTACCAAGTCAGTATTCTAAGGTTGTAAGATTACTGTAAA
AATTATATTCCAATATTTTATCTAAACTTAAGTGTGGCCAGCATTATTGGTTTCAGAGTTGCCTAAACAACACAGAATATCCTTAATTCA
GTTTGTAAAAGAGTTTTGACCAGTGTAAAAAAGTGAAGACACAGTTTCATGTTTCAAGTTTAAAATGGAAAGTTAATATCATTTGAGCAC
TTATGTGTTTTCATGTCTTCTACTTAATCTGTATGTGAAAGTAAAATATTTTTAACACCATTATTTATGCCATGTAAAGTGGGTTCTCAG
AAGCACAAGCACAGATATTTACCTTCTGAAGACATTTTTGGCTATAAAAGTGCATTGGATGGCAAACACTATTTGAGTCAGGTGTTAGAA
ATTTATTAGATGTATTATATTACATAGTAGAATAAGGTCCCTTTCTCATTTTGTTTTTCCTAAAAATAAGAAAAAAAAGAAGTGTGATAG
TAAACTGTCTTTGCTAATGTCCTGCCAGATGTTTAACTTCAAATTAAGCAAAAAGTAGGTACAATATGATGATGATCCTGATGATGATGA
TGATGATGATGATGAAGAATAAATGGAATCAAAATGCTAGCTTTATTTGACATTAATAAGTAAAATAAGTCATCATTTTTTCAACTCTGT
AGCACAGCTGTTTACATTGAATAATTTTCTCTATTGTGCTGTTAATTATATAGTAATGTATCAAAATAGAAATAGGAACTTTCTTTTCAT
TTCTTATTCATTTATGAAGAAATCTTTTCACACATAAATGATTGAATGTTCTTGTTCAGAATGACCACATAATCATTGCTTAGAAGAAGA
TACCAATTCTTTTAAAAGAAAAAAAGTCTGTTTATAATAATCAGAATCAAATGTTGTTTGTTTCTTCTAAACGTTAATGGAGAAAATTGA
AGGTGGTAAAATGTCATGTTTATTCAGGCTGGGAACTGTATTCACAGTAGAAGTTTCAGTGGTCAACATATCTATGACTCTTTAGGCTGC
TGTAGTTTTACAGTCAATTATTTAAAAGTGAGTAGTTACATTTATAAGAGCCTGAGAATACTTAGACTCAGTCATTTGTTAGTATTTTTA
CCAAAATCTCTTAGTTTCAGACATGTCAGAAGCAGCTATATAGCATATCTTATTCTATGATATACATCAGGCTATCTCAAGTTCCTGTCT
CACAGTTAATTCAAAGAAGGATTAGGATTTCTGTATTTTTTCTCATTTGAATCTTTATGTGCATTTGGTTTGTGTACATGCTTTTTGTAG

>In-frame_ENST00000310441_ENST00000273854_TCGA-LA-A446-01A_HCFC1_chrX_153236099_-_EPHA5_chr4_66189937_length(amino acids)=202AA_start in transcript=1014_stop in transcript=406
MQKHRWQVGGRHGRSHSSGKGCGGEKSTSGKGAPQFLSHTPFRLRQLSRRSGFSHSGRRLHGGRHLNCPPSLWLFPFFSLTPSPQEPPET
VERLGSRSWRPLSPVAPTTCPGAPAYKLGREALGAAILSRLSLLPSSVVALPRRKWRSPGPVPTPPSPQHWPASGAGGKEPQAPRSSQPR

--------------------------------------------------------------
>In-frame_ENST00000310441_ENST00000354839_TCGA-LA-A446-01A_HCFC1_chrX_153236099_-_EPHA5_chr4_66189937_length(transcript)=1452nt_BP=1160nt
CGGCTCCCAGGGAGGAGGCATGAGCGCCCTGCCGTTACCGCTTCTTCTGCCGTACAGTTGCCGCTTCGGGGCCTAACTCTAGCTCTTTGA
AGCGGCTCCTGTGGGAAGGAGTCATAAGGCGGGAAGTGAGGATCGGACACCGCCTTGAGTTTGGTGTGCAGTGGAAGAACTGACCTAGGA
GGAAGAGAATAGGGGGGAACGGGGAGCTAGTCTCAAGATGGCGGCTCCCACGGCGGCAGGCGCAGCCTGAGCAGCAGAGGCGGACTAAGG
GGAGAGGCGCGAGACTTCACAAGCCGTACGGCGCGAGCCCCCAAACGGCCCTCGAGAGCTCGAGCTGGTTCCACCGGCCTCCGGCCCGCC
TCCATTCGTTAGGTGTGTCGAGGCTGTTTCGGGAGCGGCGGCTGCACTAAGGCGCTGGGCGGGGAAGCGGGACGGTCTCAAGATGGCGGC
TCCCACAATTGTGGTCTGAGCGCCGGCGGGGCTGCGACGACCGCGGCGCTTGTGGCTCCTTTCCACCCGCCCCGGAAGCCGGCCAGTGCT
GGGGACTTGGGGGTGTGGGAACCGGGCCGGGGCTCCGCCATTTCCGGCGGGGGAGGGCTACGACTGAGGAAGGGAGGAGAGAGAGGCGGC
TCAAGATGGCGGCTCCCAGGGCCTCCCGCCCGAGCTTGTAAGCGGGAGCGCCCGGACAAGTAGTCGGGGCGACGGGACTCAGCGGCCTCC
AGCTTCTTGAGCCTAGGCGCTCGACAGTTTCGGGCGGCTCTTGCGGAGACGGGGTGAGCGAGAAGAAAGGGAAGAGCCAAAGGGAAGGAG
GGCAGTTAAGATGGCGGCCTCCATGGAGTCGTCTACCGCTGTGTGAGAAACCGCTTCTCCGTGAGAGCTGCCTTAGACGAAAGGGGGTGT
GTGAAAGGAATTGAGGGGCTCCCTTCCCGCTTGTTGACTTCTCCCCACCGCACCCTTTCCCGGAACTATGGCTTCGGCCGTGTCGCCCGC
CAACTTGCCAGCGGTGCTTCTGCAGCCCCGCTGGAAGCGAGTGGTGGGCTGGTCGGGTCCGGTGCCACGGCCCCGCCACGGCCACCGCGC
CGTGGCCATCAAGGAGCTCATCGTGGTGTTTGGCGGCGGCAACGAGGGAATAGTGGACGAACTGCACGTGTACAACACGGGGATTTGAGA
CGGCTTGGAGTGACTCTTGTCGGTCACCAGAAGAAGATCATGAACAGCCTTCAAGAAATGAAGGTGCAGCTGGTAAACGGAATGGTGCCA
TTGTAACTTCATGTAAATGTCGCTTCTTCAAGTGAATGATTCTGCACTTTGTAAACAGCACTGAGATTTATTTTAACAAAAAAAGGGGGA
AAAGGGAAAACAGTGATTTCTAAACCTTAGAAAACATTTGCCTCAGCCACAGAATTTGTAATCATGGTTTTACTGAAGTATCCAGTTCTT

>In-frame_ENST00000310441_ENST00000354839_TCGA-LA-A446-01A_HCFC1_chrX_153236099_-_EPHA5_chr4_66189937_length(amino acids)=202AA_start in transcript=1014_stop in transcript=406
MQKHRWQVGGRHGRSHSSGKGCGGEKSTSGKGAPQFLSHTPFRLRQLSRRSGFSHSGRRLHGGRHLNCPPSLWLFPFFSLTPSPQEPPET
VERLGSRSWRPLSPVAPTTCPGAPAYKLGREALGAAILSRLSLLPSSVVALPRRKWRSPGPVPTPPSPQHWPASGAGGKEPQAPRSSQPR

--------------------------------------------------------------
>In-frame_ENST00000369984_ENST00000273854_TCGA-LA-A446-01A_HCFC1_chrX_153236099_-_EPHA5_chr4_66189937_length(transcript)=5194nt_BP=537nt
AGGCGGCTCAAGATGGCGGCTCCCAGGGCCTCCCGCCCGAGCTTGTAAGCGGGAGCGCCCGGACAAGTAGTCGGGGCGACGGGACTCAGC
GGCCTCCAGCTTCTTGAGCCTAGGCGCTCGACAGTTTCGGGCGGCTCTTGCGGAGACGGGGTGAGCGAGAAGAAAGGGAAGAGCCAAAGG
GAAGGAGGGCAGTTAAGATGGCGGCCTCCATGGAGTCGTCTACCGCTGTGTGAGAAACCGCTTCTCCGTGAGAGCTGCCTTAGACGAAAG
GGGGTGTGTGAAAGGAATTGAGGGGCTCCCTTCCCGCTTGTTGACTTCTCCCCACCGCACCCTTTCCCGGAACTATGGCTTCGGCCGTGT
CGCCCGCCAACTTGCCAGCGGTGCTTCTGCAGCCCCGCTGGAAGCGAGTGGTGGGCTGGTCGGGTCCGGTGCCACGGCCCCGCCACGGCC
ACCGCGCCGTGGCCATCAAGGAGCTCATCGTGGTGTTTGGCGGCGGCAACGAGGGAATAGTGGACGAACTGCACGTGTACAACACGGGGA
TTTGAGACGGCTTGGAGTGACTCTTGTCGGTCACCAGAAGAAGATCATGAACAGCCTTCAAGAAATGAAGGTGCAGCTGGTAAACGGAAT
GGTGCCATTGTAACTTCATGTAAATGTCGCTTCTTCAAGTGAATGATTCTGCACTTTGTAAACAGCACTGAGATTTATTTTAACAAAAAA
AGGGGGAAAAGGGAAAACAGTGATTTCTAAACCTTAGAAAACATTTGCCTCAGCCACAGAATTTGTAATCATGGTTTTACTGAAGTATCC
AGTTCTTAGTCCTTAGTCTTCATTTTTCATGAAGCAAACATATCTTGCATTAAAAGGGACATGAAGTTAGACATCATCTTAAGTTACAAC
AACAGAATCCTTCCCACTACTTCTACAAAATTTTGTACATGAAATATATAATTATATAGCACTTTTATAGACTGAATTAAGGCAACCCCT
TTCAAAACTTCCAGGGATCTACTTGAAAGGAAATGTTTTATAGCCATTTGTGAGCTAACAAAAGCTACAGTTTACTGAAGTTTACTTCAA
GTCTTAATTGTCTACAAAAGTGTATTGAAGAGCAATATGATTAGATTATTTCTTAATAGATATCTTCTTTTGTAATTTTAAAATGCTGTT
ACACAGCGTTAAGTTATAGAAACTAGTGTGTAAACATGTTGCTTGATCAAGAAAAAGTACAATACAGGGTGTATATTTATTTTTTGTGTT
ATAAAGTTTACTTTTAGTTGCTCTTCTAGAGATTATTAGGTAATAAATGTGTATATACTGTATAATTTGCAATATACCCAGGAACTGATT
TAAGATGGAATTGTGTGTGTGTTTGCTTGCACATGTGTGTGTTACCATTCTGCTTGCATTTCTAATAGTGTTTCAATTTTAGCAACATAT
AGGAGCAAGTGTTCCAGAATGTAATATGAATAGGAGAAATAGGGAAGCAGTAAAACAAAATTTAACACAAGCTTGTGTCTCTTTTCCTCT
CATGTGTCCAAAAGCTAATCTCTTTATTCACTAAAAGGAAATGTGTATAAGACTAAATCCCCTTTGGCTTTTTAAAAACATTTTGTGATA
TCAGTGACAATGCAGTTCTTCTAGCCATTAATCTTGTACCCCTGTAAGAAATTTCACCTTTCTGAGTCCTGAAAAGTATCTTGTCAAGCA
AAGTTGACACCGAAGGGCACATTTTCAGCAGGATGTAGAAGGATTTAACTGTGCAGGCTTCTGTTAATGTTGTTAAATCCAGGCACATAG
CACGAAGCATCACCCTTAAGTGTTAATCCGTTGTAACCATTCCCATTTTGACTCAGTTCTAGAAATTTTGACTCTAAGGCAGCAATGGAA
TTATGACAAATATATATATATACACATACACACACACAAATATATATATATGCTTATTTCCCTTCAGAATTTTATTTCAATAATTGATAA
GTTTTATTTTTAATGGATGTTTATTCATTTGTTAATTTCAGTTTGTATTCAGTTACATGGCTTTGAGTTTATTTTATTGAACCAATCCAA
GGTATTATGTAATTAGGCTTATTAAGGAATATAACATATCTTCTATGTATGCTCTATATACCACACATGATGAAGACTTATATTTGTGAT
ACAAATTATGCAGTGTATTAGAAATTGTCCAAAACTGAGCTCCTGAGATAACCAACATTTTTGTATTATTACACTTTATAAAAATTATGG
AATGTCATGTGATTAGTCAAAATCAGAGCATTTAAATAAAATACAGCCTACTCATGATTATCAATGTTAATAGTACAGTAGATCAGAGAA
GCCTGGATTAATGAAATTTATCAGAAATATAGCCACGTTATATTAATGGTATTCTTTTTTCCAAGCACTTGTTTTATTTGAATATTAGCA
CATATAAGAATAAACTTTTGGGAAATAACTACTTCCTATTCTGCCCACCCTTTACTTAAGCAGTAAAGTACCTCTTTTCTAAAGAACCAG
GAAACATCAAAATAACCACAACAAAAATCTAAACAGTTTTCAAATAATACATTCAGTTCTCATAATAATAAGGGCTATATACCATGGGTC
CACACAAACTGTGCTGCAAACATATAATCTATTAGTTTAGTAAAATTTCCAGAGATGTGAATTTAATATTTTCTTGATAAAATCATAAAA
AAGCATCACCATTATTAAAGATGCATTTGTTCATTTCAATAAACAGAAATTAATGAAACAAATTACTTTTGTACAAAATAAATGATGATT
ATGTGGCACTTTATTGAATTTTATAGAAATATTCCAATCTAAGTGATAAAGTAACAACTGAGAAACTTCAGAATGAAAGCCTTATTGATT
CAAAAGGAAAATTACCATATGCTTTAGGGCTGATGAACTTGCCACAATTGCTTAGACATAACAAAGATATTTTCTACATTGTTTTCCAAC
TTTATATGCTGAAAGATAATTAGGAGGTCCGGGTGGAGTGTATCTAAATGACTAGAACTTTAAGTTGCAATATAGATTTTCTCTTTTTAA
CAAAGGAAGAATATAAATTAATTTGGATCAAATTTATTTGCCTTCTTTGCAATCTTGGTGATCATTTTGGAAAGTAAATTGAAAGGAAAG
TTAAATAGCCACATAGTTTTCTTTTGCATCTCAATTTGGTTGAGAATTTCTAAGGAAGGTTAATATGACTTTAGAATGAATAAAGAGACT
GTCAAACAACAAGTACTCTCCCTAAAAAAGAGGAAAGAATGCCTTCCTAAAATATTTGTTTCCTATTGTATATGTCACCAATTGGAAAAT
GCCAATCTCATCTGCAAGTGTGAGAAATGGAACAAGGAGAGGAGCCAACTCTAGTAAAATGTAGTGAGGCCATAATCCAAGGCAAAGTTT
AACAAATCTTGATTGTGTAATTTCTTATTCTGTTGATTCATTTTGTCACCCACCAGACTACCAAAAATAAAGCACAGACATGACAATTTT
AGTATATACAACAGATTTGGCAAATAATACATAATCTTATTGTAATTGCCAGTAGCAATATCTTAAGGGGCATGAGTCTTTCACAGTATT
GGACTTTGAAAAATTGGTACAGGGAGTAATTTTCAACAGACTGATACTTGAAGTCACTCAATTACTACTTTGACCTATCATAATTTGTTT
CTAATCTTGTCATTTGCTCATATATACTATTAATATAACCAGTTGCTCTTTTCATAATGTACTATTTCTATGTAAAGATGTAAAGATTTT
GATTTTCCTATGTTTTAAAATTGTAATGTGCATGATCTTTTATTTCTTGTGTTTCAATTACCAAGTCAGTATTCTAAGGTTGTAAGATTA
CTGTAAAAATTATATTCCAATATTTTATCTAAACTTAAGTGTGGCCAGCATTATTGGTTTCAGAGTTGCCTAAACAACACAGAATATCCT
TAATTCAGTTTGTAAAAGAGTTTTGACCAGTGTAAAAAAGTGAAGACACAGTTTCATGTTTCAAGTTTAAAATGGAAAGTTAATATCATT
TGAGCACTTATGTGTTTTCATGTCTTCTACTTAATCTGTATGTGAAAGTAAAATATTTTTAACACCATTATTTATGCCATGTAAAGTGGG
TTCTCAGAAGCACAAGCACAGATATTTACCTTCTGAAGACATTTTTGGCTATAAAAGTGCATTGGATGGCAAACACTATTTGAGTCAGGT
GTTAGAAATTTATTAGATGTATTATATTACATAGTAGAATAAGGTCCCTTTCTCATTTTGTTTTTCCTAAAAATAAGAAAAAAAAGAAGT
GTGATAGTAAACTGTCTTTGCTAATGTCCTGCCAGATGTTTAACTTCAAATTAAGCAAAAAGTAGGTACAATATGATGATGATCCTGATG
ATGATGATGATGATGATGATGAAGAATAAATGGAATCAAAATGCTAGCTTTATTTGACATTAATAAGTAAAATAAGTCATCATTTTTTCA
ACTCTGTAGCACAGCTGTTTACATTGAATAATTTTCTCTATTGTGCTGTTAATTATATAGTAATGTATCAAAATAGAAATAGGAACTTTC
TTTTCATTTCTTATTCATTTATGAAGAAATCTTTTCACACATAAATGATTGAATGTTCTTGTTCAGAATGACCACATAATCATTGCTTAG
AAGAAGATACCAATTCTTTTAAAAGAAAAAAAGTCTGTTTATAATAATCAGAATCAAATGTTGTTTGTTTCTTCTAAACGTTAATGGAGA
AAATTGAAGGTGGTAAAATGTCATGTTTATTCAGGCTGGGAACTGTATTCACAGTAGAAGTTTCAGTGGTCAACATATCTATGACTCTTT
AGGCTGCTGTAGTTTTACAGTCAATTATTTAAAAGTGAGTAGTTACATTTATAAGAGCCTGAGAATACTTAGACTCAGTCATTTGTTAGT
ATTTTTACCAAAATCTCTTAGTTTCAGACATGTCAGAAGCAGCTATATAGCATATCTTATTCTATGATATACATCAGGCTATCTCAAGTT
CCTGTCTCACAGTTAATTCAAAGAAGGATTAGGATTTCTGTATTTTTTCTCATTTGAATCTTTATGTGCATTTGGTTTGTGTACATGCTT

>In-frame_ENST00000369984_ENST00000273854_TCGA-LA-A446-01A_HCFC1_chrX_153236099_-_EPHA5_chr4_66189937_length(amino acids)=129AA_start in transcript=648_stop in transcript=259
MKLQWHHSVYQLHLHFLKAVHDLLLVTDKSHSKPSQIPVLYTCSSSTIPSLPPPNTTMSSLMATARWPWRGRGTGPDQPTTRFQRGCRST

--------------------------------------------------------------
>In-frame_ENST00000369984_ENST00000354839_TCGA-LA-A446-01A_HCFC1_chrX_153236099_-_EPHA5_chr4_66189937_length(transcript)=829nt_BP=537nt
AGGCGGCTCAAGATGGCGGCTCCCAGGGCCTCCCGCCCGAGCTTGTAAGCGGGAGCGCCCGGACAAGTAGTCGGGGCGACGGGACTCAGC
GGCCTCCAGCTTCTTGAGCCTAGGCGCTCGACAGTTTCGGGCGGCTCTTGCGGAGACGGGGTGAGCGAGAAGAAAGGGAAGAGCCAAAGG
GAAGGAGGGCAGTTAAGATGGCGGCCTCCATGGAGTCGTCTACCGCTGTGTGAGAAACCGCTTCTCCGTGAGAGCTGCCTTAGACGAAAG
GGGGTGTGTGAAAGGAATTGAGGGGCTCCCTTCCCGCTTGTTGACTTCTCCCCACCGCACCCTTTCCCGGAACTATGGCTTCGGCCGTGT
CGCCCGCCAACTTGCCAGCGGTGCTTCTGCAGCCCCGCTGGAAGCGAGTGGTGGGCTGGTCGGGTCCGGTGCCACGGCCCCGCCACGGCC
ACCGCGCCGTGGCCATCAAGGAGCTCATCGTGGTGTTTGGCGGCGGCAACGAGGGAATAGTGGACGAACTGCACGTGTACAACACGGGGA
TTTGAGACGGCTTGGAGTGACTCTTGTCGGTCACCAGAAGAAGATCATGAACAGCCTTCAAGAAATGAAGGTGCAGCTGGTAAACGGAAT
GGTGCCATTGTAACTTCATGTAAATGTCGCTTCTTCAAGTGAATGATTCTGCACTTTGTAAACAGCACTGAGATTTATTTTAACAAAAAA
AGGGGGAAAAGGGAAAACAGTGATTTCTAAACCTTAGAAAACATTTGCCTCAGCCACAGAATTTGTAATCATGGTTTTACTGAAGTATCC

>In-frame_ENST00000369984_ENST00000354839_TCGA-LA-A446-01A_HCFC1_chrX_153236099_-_EPHA5_chr4_66189937_length(amino acids)=129AA_start in transcript=648_stop in transcript=259
MKLQWHHSVYQLHLHFLKAVHDLLLVTDKSHSKPSQIPVLYTCSSSTIPSLPPPNTTMSSLMATARWPWRGRGTGPDQPTTRFQRGCRST

--------------------------------------------------------------
>In-frame_ENST00000354233_ENST00000273854_TCGA-LA-A446-01A_HCFC1_chrX_153236099_-_EPHA5_chr4_66189937_length(transcript)=5378nt_BP=721nt
AAGATGGCGGCTCCCACAATTGTGGTCTGAGCGCCGGCGGGGCTGCGACGACCGCGGCGCTTGTGGCTCCTTTCCACCCGCCCCGGAAGC
CGGCCAGTGCTGGGGACTTGGGGGTGTGGGAACCGGGCCGGGGCTCCGCCATTTCCGGCGGGGGAGGGCTACGACTGAGGAAGGGAGGAG
AGAGAGGCGGCTCAAGATGGCGGCTCCCAGGGCCTCCCGCCCGAGCTTGTAAGCGGGAGCGCCCGGACAAGTAGTCGGGGCGACGGGACT
CAGCGGCCTCCAGCTTCTTGAGCCTAGGCGCTCGACAGTTTCGGGCGGCTCTTGCGGAGACGGGGTGAGCGAGAAGAAAGGGAAGAGCCA
AAGGGAAGGAGGGCAGTTAAGATGGCGGCCTCCATGGAGTCGTCTACCGCTGTGTGAGAAACCGCTTCTCCGTGAGAGCTGCCTTAGACG
AAAGGGGGTGTGTGAAAGGAATTGAGGGGCTCCCTTCCCGCTTGTTGACTTCTCCCCACCGCACCCTTTCCCGGAACTATGGCTTCGGCC
GTGTCGCCCGCCAACTTGCCAGCGGTGCTTCTGCAGCCCCGCTGGAAGCGAGTGGTGGGCTGGTCGGGTCCGGTGCCACGGCCCCGCCAC
GGCCACCGCGCCGTGGCCATCAAGGAGCTCATCGTGGTGTTTGGCGGCGGCAACGAGGGAATAGTGGACGAACTGCACGTGTACAACACG
GGGATTTGAGACGGCTTGGAGTGACTCTTGTCGGTCACCAGAAGAAGATCATGAACAGCCTTCAAGAAATGAAGGTGCAGCTGGTAAACG
GAATGGTGCCATTGTAACTTCATGTAAATGTCGCTTCTTCAAGTGAATGATTCTGCACTTTGTAAACAGCACTGAGATTTATTTTAACAA
AAAAAGGGGGAAAAGGGAAAACAGTGATTTCTAAACCTTAGAAAACATTTGCCTCAGCCACAGAATTTGTAATCATGGTTTTACTGAAGT
ATCCAGTTCTTAGTCCTTAGTCTTCATTTTTCATGAAGCAAACATATCTTGCATTAAAAGGGACATGAAGTTAGACATCATCTTAAGTTA
CAACAACAGAATCCTTCCCACTACTTCTACAAAATTTTGTACATGAAATATATAATTATATAGCACTTTTATAGACTGAATTAAGGCAAC
CCCTTTCAAAACTTCCAGGGATCTACTTGAAAGGAAATGTTTTATAGCCATTTGTGAGCTAACAAAAGCTACAGTTTACTGAAGTTTACT
TCAAGTCTTAATTGTCTACAAAAGTGTATTGAAGAGCAATATGATTAGATTATTTCTTAATAGATATCTTCTTTTGTAATTTTAAAATGC
TGTTACACAGCGTTAAGTTATAGAAACTAGTGTGTAAACATGTTGCTTGATCAAGAAAAAGTACAATACAGGGTGTATATTTATTTTTTG
TGTTATAAAGTTTACTTTTAGTTGCTCTTCTAGAGATTATTAGGTAATAAATGTGTATATACTGTATAATTTGCAATATACCCAGGAACT
GATTTAAGATGGAATTGTGTGTGTGTTTGCTTGCACATGTGTGTGTTACCATTCTGCTTGCATTTCTAATAGTGTTTCAATTTTAGCAAC
ATATAGGAGCAAGTGTTCCAGAATGTAATATGAATAGGAGAAATAGGGAAGCAGTAAAACAAAATTTAACACAAGCTTGTGTCTCTTTTC
CTCTCATGTGTCCAAAAGCTAATCTCTTTATTCACTAAAAGGAAATGTGTATAAGACTAAATCCCCTTTGGCTTTTTAAAAACATTTTGT
GATATCAGTGACAATGCAGTTCTTCTAGCCATTAATCTTGTACCCCTGTAAGAAATTTCACCTTTCTGAGTCCTGAAAAGTATCTTGTCA
AGCAAAGTTGACACCGAAGGGCACATTTTCAGCAGGATGTAGAAGGATTTAACTGTGCAGGCTTCTGTTAATGTTGTTAAATCCAGGCAC
ATAGCACGAAGCATCACCCTTAAGTGTTAATCCGTTGTAACCATTCCCATTTTGACTCAGTTCTAGAAATTTTGACTCTAAGGCAGCAAT
GGAATTATGACAAATATATATATATACACATACACACACACAAATATATATATATGCTTATTTCCCTTCAGAATTTTATTTCAATAATTG
ATAAGTTTTATTTTTAATGGATGTTTATTCATTTGTTAATTTCAGTTTGTATTCAGTTACATGGCTTTGAGTTTATTTTATTGAACCAAT
CCAAGGTATTATGTAATTAGGCTTATTAAGGAATATAACATATCTTCTATGTATGCTCTATATACCACACATGATGAAGACTTATATTTG
TGATACAAATTATGCAGTGTATTAGAAATTGTCCAAAACTGAGCTCCTGAGATAACCAACATTTTTGTATTATTACACTTTATAAAAATT
ATGGAATGTCATGTGATTAGTCAAAATCAGAGCATTTAAATAAAATACAGCCTACTCATGATTATCAATGTTAATAGTACAGTAGATCAG
AGAAGCCTGGATTAATGAAATTTATCAGAAATATAGCCACGTTATATTAATGGTATTCTTTTTTCCAAGCACTTGTTTTATTTGAATATT
AGCACATATAAGAATAAACTTTTGGGAAATAACTACTTCCTATTCTGCCCACCCTTTACTTAAGCAGTAAAGTACCTCTTTTCTAAAGAA
CCAGGAAACATCAAAATAACCACAACAAAAATCTAAACAGTTTTCAAATAATACATTCAGTTCTCATAATAATAAGGGCTATATACCATG
GGTCCACACAAACTGTGCTGCAAACATATAATCTATTAGTTTAGTAAAATTTCCAGAGATGTGAATTTAATATTTTCTTGATAAAATCAT
AAAAAAGCATCACCATTATTAAAGATGCATTTGTTCATTTCAATAAACAGAAATTAATGAAACAAATTACTTTTGTACAAAATAAATGAT
GATTATGTGGCACTTTATTGAATTTTATAGAAATATTCCAATCTAAGTGATAAAGTAACAACTGAGAAACTTCAGAATGAAAGCCTTATT
GATTCAAAAGGAAAATTACCATATGCTTTAGGGCTGATGAACTTGCCACAATTGCTTAGACATAACAAAGATATTTTCTACATTGTTTTC
CAACTTTATATGCTGAAAGATAATTAGGAGGTCCGGGTGGAGTGTATCTAAATGACTAGAACTTTAAGTTGCAATATAGATTTTCTCTTT
TTAACAAAGGAAGAATATAAATTAATTTGGATCAAATTTATTTGCCTTCTTTGCAATCTTGGTGATCATTTTGGAAAGTAAATTGAAAGG
AAAGTTAAATAGCCACATAGTTTTCTTTTGCATCTCAATTTGGTTGAGAATTTCTAAGGAAGGTTAATATGACTTTAGAATGAATAAAGA
GACTGTCAAACAACAAGTACTCTCCCTAAAAAAGAGGAAAGAATGCCTTCCTAAAATATTTGTTTCCTATTGTATATGTCACCAATTGGA
AAATGCCAATCTCATCTGCAAGTGTGAGAAATGGAACAAGGAGAGGAGCCAACTCTAGTAAAATGTAGTGAGGCCATAATCCAAGGCAAA
GTTTAACAAATCTTGATTGTGTAATTTCTTATTCTGTTGATTCATTTTGTCACCCACCAGACTACCAAAAATAAAGCACAGACATGACAA
TTTTAGTATATACAACAGATTTGGCAAATAATACATAATCTTATTGTAATTGCCAGTAGCAATATCTTAAGGGGCATGAGTCTTTCACAG
TATTGGACTTTGAAAAATTGGTACAGGGAGTAATTTTCAACAGACTGATACTTGAAGTCACTCAATTACTACTTTGACCTATCATAATTT
GTTTCTAATCTTGTCATTTGCTCATATATACTATTAATATAACCAGTTGCTCTTTTCATAATGTACTATTTCTATGTAAAGATGTAAAGA
TTTTGATTTTCCTATGTTTTAAAATTGTAATGTGCATGATCTTTTATTTCTTGTGTTTCAATTACCAAGTCAGTATTCTAAGGTTGTAAG
ATTACTGTAAAAATTATATTCCAATATTTTATCTAAACTTAAGTGTGGCCAGCATTATTGGTTTCAGAGTTGCCTAAACAACACAGAATA
TCCTTAATTCAGTTTGTAAAAGAGTTTTGACCAGTGTAAAAAAGTGAAGACACAGTTTCATGTTTCAAGTTTAAAATGGAAAGTTAATAT
CATTTGAGCACTTATGTGTTTTCATGTCTTCTACTTAATCTGTATGTGAAAGTAAAATATTTTTAACACCATTATTTATGCCATGTAAAG
TGGGTTCTCAGAAGCACAAGCACAGATATTTACCTTCTGAAGACATTTTTGGCTATAAAAGTGCATTGGATGGCAAACACTATTTGAGTC
AGGTGTTAGAAATTTATTAGATGTATTATATTACATAGTAGAATAAGGTCCCTTTCTCATTTTGTTTTTCCTAAAAATAAGAAAAAAAAG
AAGTGTGATAGTAAACTGTCTTTGCTAATGTCCTGCCAGATGTTTAACTTCAAATTAAGCAAAAAGTAGGTACAATATGATGATGATCCT
GATGATGATGATGATGATGATGATGAAGAATAAATGGAATCAAAATGCTAGCTTTATTTGACATTAATAAGTAAAATAAGTCATCATTTT
TTCAACTCTGTAGCACAGCTGTTTACATTGAATAATTTTCTCTATTGTGCTGTTAATTATATAGTAATGTATCAAAATAGAAATAGGAAC
TTTCTTTTCATTTCTTATTCATTTATGAAGAAATCTTTTCACACATAAATGATTGAATGTTCTTGTTCAGAATGACCACATAATCATTGC
TTAGAAGAAGATACCAATTCTTTTAAAAGAAAAAAAGTCTGTTTATAATAATCAGAATCAAATGTTGTTTGTTTCTTCTAAACGTTAATG
GAGAAAATTGAAGGTGGTAAAATGTCATGTTTATTCAGGCTGGGAACTGTATTCACAGTAGAAGTTTCAGTGGTCAACATATCTATGACT
CTTTAGGCTGCTGTAGTTTTACAGTCAATTATTTAAAAGTGAGTAGTTACATTTATAAGAGCCTGAGAATACTTAGACTCAGTCATTTGT
TAGTATTTTTACCAAAATCTCTTAGTTTCAGACATGTCAGAAGCAGCTATATAGCATATCTTATTCTATGATATACATCAGGCTATCTCA
AGTTCCTGTCTCACAGTTAATTCAAAGAAGGATTAGGATTTCTGTATTTTTTCTCATTTGAATCTTTATGTGCATTTGGTTTGTGTACAT

>In-frame_ENST00000354233_ENST00000273854_TCGA-LA-A446-01A_HCFC1_chrX_153236099_-_EPHA5_chr4_66189937_length(amino acids)=129AA_start in transcript=832_stop in transcript=443
MKLQWHHSVYQLHLHFLKAVHDLLLVTDKSHSKPSQIPVLYTCSSSTIPSLPPPNTTMSSLMATARWPWRGRGTGPDQPTTRFQRGCRST

--------------------------------------------------------------
>In-frame_ENST00000354233_ENST00000354839_TCGA-LA-A446-01A_HCFC1_chrX_153236099_-_EPHA5_chr4_66189937_length(transcript)=1013nt_BP=721nt
AAGATGGCGGCTCCCACAATTGTGGTCTGAGCGCCGGCGGGGCTGCGACGACCGCGGCGCTTGTGGCTCCTTTCCACCCGCCCCGGAAGC
CGGCCAGTGCTGGGGACTTGGGGGTGTGGGAACCGGGCCGGGGCTCCGCCATTTCCGGCGGGGGAGGGCTACGACTGAGGAAGGGAGGAG
AGAGAGGCGGCTCAAGATGGCGGCTCCCAGGGCCTCCCGCCCGAGCTTGTAAGCGGGAGCGCCCGGACAAGTAGTCGGGGCGACGGGACT
CAGCGGCCTCCAGCTTCTTGAGCCTAGGCGCTCGACAGTTTCGGGCGGCTCTTGCGGAGACGGGGTGAGCGAGAAGAAAGGGAAGAGCCA
AAGGGAAGGAGGGCAGTTAAGATGGCGGCCTCCATGGAGTCGTCTACCGCTGTGTGAGAAACCGCTTCTCCGTGAGAGCTGCCTTAGACG
AAAGGGGGTGTGTGAAAGGAATTGAGGGGCTCCCTTCCCGCTTGTTGACTTCTCCCCACCGCACCCTTTCCCGGAACTATGGCTTCGGCC
GTGTCGCCCGCCAACTTGCCAGCGGTGCTTCTGCAGCCCCGCTGGAAGCGAGTGGTGGGCTGGTCGGGTCCGGTGCCACGGCCCCGCCAC
GGCCACCGCGCCGTGGCCATCAAGGAGCTCATCGTGGTGTTTGGCGGCGGCAACGAGGGAATAGTGGACGAACTGCACGTGTACAACACG
GGGATTTGAGACGGCTTGGAGTGACTCTTGTCGGTCACCAGAAGAAGATCATGAACAGCCTTCAAGAAATGAAGGTGCAGCTGGTAAACG
GAATGGTGCCATTGTAACTTCATGTAAATGTCGCTTCTTCAAGTGAATGATTCTGCACTTTGTAAACAGCACTGAGATTTATTTTAACAA
AAAAAGGGGGAAAAGGGAAAACAGTGATTTCTAAACCTTAGAAAACATTTGCCTCAGCCACAGAATTTGTAATCATGGTTTTACTGAAGT

>In-frame_ENST00000354233_ENST00000354839_TCGA-LA-A446-01A_HCFC1_chrX_153236099_-_EPHA5_chr4_66189937_length(amino acids)=129AA_start in transcript=832_stop in transcript=443
MKLQWHHSVYQLHLHFLKAVHDLLLVTDKSHSKPSQIPVLYTCSSSTIPSLPPPNTTMSSLMATARWPWRGRGTGPDQPTTRFQRGCRST

--------------------------------------------------------------

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Fusion Gene PPI Analysis for HCFC1-EPHA5

Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in
ChiPPI page.

Protein-protein interactors with each fusion partner protein in wild-type (BIOGRID-3.4.160)

Hgene

Hgene's interactors

Tgene

Tgene's interactors

- Retained PPIs in in-frame fusion.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Still interaction with

- Lost PPIs in in-frame fusion.

Partner	Gene	Hbp	Tbp	ENST	Strand	BPexon	TotalExon	Protein feature loci	*BPloci	TotalLen	Interaction lost with
Hgene	HCFC1	chrX:153236099	chr4:66189937	ENST00000310441	-	1	26	813_912	64.33333333333333	2036.0	GABP2
Hgene	HCFC1	chrX:153236099	chr4:66189937	ENST00000354233	-	1	27	813_912	64.33333333333333	1967.0	GABP2
Hgene	HCFC1	chrX:153236099	chr4:66189937	ENST00000310441	-	1	26	610_722	64.33333333333333	2036.0	SIN3A
Hgene	HCFC1	chrX:153236099	chr4:66189937	ENST00000354233	-	1	27	610_722	64.33333333333333	1967.0	SIN3A
Hgene	HCFC1	chrX:153236099	chr4:66189937	ENST00000310441	-	1	26	750_902	64.33333333333333	2036.0	ZBTB17
Hgene	HCFC1	chrX:153236099	chr4:66189937	ENST00000354233	-	1	27	750_902	64.33333333333333	1967.0	ZBTB17

- Retained PPIs, but lost function due to frame-shift fusion.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Interaction lost with

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Related Drugs for HCFC1-EPHA5

Drugs targeting genes involved in this fusion gene.
(DrugBank Version 5.1.8 2021-05-08)

Partner	Gene	UniProtAcc	DrugBank ID	Drug name	Drug activity	Drug type	Drug status
Tgene	EPHA5	P54756	DB01254	Dasatinib		Small molecule	Approved\|Investigational
Tgene	EPHA5	P54756	DB01254	Dasatinib		Small molecule	Approved\|Investigational
Tgene	EPHA5	P54756	DB01254	Dasatinib		Small molecule	Approved\|Investigational
Tgene	EPHA5	P54756	DB01254	Dasatinib		Small molecule	Approved\|Investigational
Tgene	EPHA5	P54756	DB01254	Dasatinib		Small molecule	Approved\|Investigational
Tgene	EPHA5	P54756	DB12010	Fostamatinib	Inhibitor	Small molecule	Approved\|Investigational
Tgene	EPHA5	P54756	DB12010	Fostamatinib	Inhibitor	Small molecule	Approved\|Investigational
Tgene	EPHA5	P54756	DB12010	Fostamatinib	Inhibitor	Small molecule	Approved\|Investigational
Tgene	EPHA5	P54756	DB12010	Fostamatinib	Inhibitor	Small molecule	Approved\|Investigational
Tgene	EPHA5	P54756	DB12010	Fostamatinib	Inhibitor	Small molecule	Approved\|Investigational

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Related Diseases for HCFC1-EPHA5

Diseases associated with fusion partners.
(DisGeNet 4.0)

Partner	Gene	Disease ID	Disease name	# pubmeds	Source
Hgene	HCFC1	C0796208	MENTAL RETARDATION, X-LINKED 3	2	CTD_human;GENOMICS_ENGLAND;ORPHANET;UNIPROT
Hgene	HCFC1	C0023903	Liver neoplasms	1	CTD_human
Hgene	HCFC1	C0345904	Malignant neoplasm of liver	1	CTD_human
Hgene	HCFC1	C2931498	Mental Retardation, X-Linked 1	1	ORPHANET