FusionGDB Logo

Home

Download

Statistics

Examples

Help

Contact
Center for Computational Systems Medicine
leaf

Fusion Gene Summary

leaf

Fusion Gene ORF analysis

leaf

Fusion Genomic Features

leaf

Fusion Protein Features

leaf

Fusion Gene Sequence

leaf

Fusion Gene PPI analysis

leaf

Related Drugs

leaf

Related Diseases

Fusion gene:HLCS-GRIK1 (FusionGDB2 ID:36580)

Fusion Gene Summary for HLCS-GRIK1

check button Fusion gene summary
Fusion gene informationFusion gene name: HLCS-GRIK1
Fusion gene ID: 36580
HgeneTgene
Gene symbol

HLCS

GRIK1

Gene ID

3141

2897

Gene nameholocarboxylase synthetaseglutamate ionotropic receptor kainate type subunit 1
SynonymsHCSEAA3|EEA3|GLR5|GLUR5|GluK1|gluR-5
Cytomap

21q22.13

21q21.3

Type of geneprotein-codingprotein-coding
Descriptionbiotin--protein ligasebiotin apo-protein ligasebiotin--[acetyl-CoA-carboxylase] ligasebiotin--[methylcrotonoyl-CoA-carboxylase] ligasebiotin--[methylmalonyl-CoA-carboxytransferase] ligaseholocarboxylase synthetase (biotin-(proprionyl-CoA-carboxylase glutamate receptor ionotropic, kainate 1excitatory amino acid receptor 3glutamate receptor 5
Modification date2020031320200320
UniProtAcc.

P39086

Ensembl transtripts involved in fusion geneENST00000336648, ENST00000399120, 
ENST00000482273, 		ENST00000327783, 
ENST00000389125, ENST00000399913, 
ENST00000399914, ENST00000535441, 
ENST00000389124, ENST00000399907, 
ENST00000399909, ENST00000309434, 
ENST00000472429, 
Fusion gene scores* DoF score17 X 9 X 9=137712 X 9 X 6=648
# samples 1712
** MAII scorelog2(17/1377*10)=-3.01792190799726
possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs).
DoF>8 and MAII<0		log2(12/648*10)=-2.43295940727611
possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs).
DoF>8 and MAII<0
Context

PubMed: HLCS [Title/Abstract] AND GRIK1 [Title/Abstract] AND fusion [Title/Abstract]

Most frequent breakpointHLCS(38269160)-GRIK1(30971257), # samples:3
Anticipated loss of major functional domain due to fusion event.HLCS-GRIK1 seems lost the major protein functional domain in Hgene partner, which is a essential gene due to the frame-shifted ORF.
HLCS-GRIK1 seems lost the major protein functional domain in Hgene partner, which is a epigenetic factor due to the frame-shifted ORF.
HLCS-GRIK1 seems lost the major protein functional domain in Tgene partner, which is a IUPHAR drug target due to the frame-shifted ORF.
* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

check button Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez
PartnerGeneGO IDGO termPubMed ID
HgeneHLCS

GO:0009305

protein biotinylation

7842009

HgeneHLCS

GO:0016570

histone modification

14613969

HgeneHLCS

GO:0070781

response to biotin

17904341

HgeneHLCS

GO:0071110

histone biotinylation

14613969


check buttonFusion gene breakpoints across HLCS (5'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

check buttonFusion gene breakpoints across GRIK1 (3'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

check button Fusion gene information from two resources (ChiTars 5.0 and ChimerDB 4.0)
* All genome coordinats were lifted-over on hg19.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.
SourceDiseaseSampleHgeneHchrHbpHstrandTgeneTchrTbpTstrand
ChimerDB4LUADTCGA-50-5944-01AHLCSchr21

38269160

-GRIK1chr21

30971257

-
ChimerDB4LUADTCGA-50-5944-01AHLCSchr21

38269160

-GRIK1chr21

30971257

-
ChimerDB4LUADTCGA-50-5944-01AHLCSchr21

38269160

-GRIK1chr21

30971257

-


Top

Fusion Gene ORF analysis for HLCS-GRIK1

check button Open reading frame (ORF) analsis of fusion genes based on Ensembl gene isoform structure.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.
ORFHenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrand
In-frameENST00000336648ENST00000327783HLCSchr21

38269160

-GRIK1chr21

30971257

-
In-frameENST00000336648ENST00000389125HLCSchr21

38269160

-GRIK1chr21

30971257

-
In-frameENST00000336648ENST00000399913HLCSchr21

38269160

-GRIK1chr21

30971257

-
In-frameENST00000336648ENST00000399914HLCSchr21

38269160

-GRIK1chr21

30971257

-
In-frameENST00000336648ENST00000535441HLCSchr21

38269160

-GRIK1chr21

30971257

-
In-frameENST00000336648ENST00000389124HLCSchr21

38269160

-GRIK1chr21

30971257

-
In-frameENST00000336648ENST00000399907HLCSchr21

38269160

-GRIK1chr21

30971257

-
In-frameENST00000336648ENST00000399909HLCSchr21

38269160

-GRIK1chr21

30971257

-
In-frameENST00000336648ENST00000309434HLCSchr21

38269160

-GRIK1chr21

30971257

-
5CDS-5UTRENST00000336648ENST00000472429HLCSchr21

38269160

-GRIK1chr21

30971257

-
Frame-shiftENST00000399120ENST00000327783HLCSchr21

38269160

-GRIK1chr21

30971257

-
Frame-shiftENST00000399120ENST00000389125HLCSchr21

38269160

-GRIK1chr21

30971257

-
Frame-shiftENST00000399120ENST00000399913HLCSchr21

38269160

-GRIK1chr21

30971257

-
Frame-shiftENST00000399120ENST00000399914HLCSchr21

38269160

-GRIK1chr21

30971257

-
Frame-shiftENST00000399120ENST00000535441HLCSchr21

38269160

-GRIK1chr21

30971257

-
Frame-shiftENST00000399120ENST00000389124HLCSchr21

38269160

-GRIK1chr21

30971257

-
Frame-shiftENST00000399120ENST00000399907HLCSchr21

38269160

-GRIK1chr21

30971257

-
Frame-shiftENST00000399120ENST00000399909HLCSchr21

38269160

-GRIK1chr21

30971257

-
Frame-shiftENST00000399120ENST00000309434HLCSchr21

38269160

-GRIK1chr21

30971257

-
5CDS-5UTRENST00000399120ENST00000472429HLCSchr21

38269160

-GRIK1chr21

30971257

-
intron-3CDSENST00000482273ENST00000327783HLCSchr21

38269160

-GRIK1chr21

30971257

-
intron-3CDSENST00000482273ENST00000389125HLCSchr21

38269160

-GRIK1chr21

30971257

-
intron-3CDSENST00000482273ENST00000399913HLCSchr21

38269160

-GRIK1chr21

30971257

-
intron-3CDSENST00000482273ENST00000399914HLCSchr21

38269160

-GRIK1chr21

30971257

-
intron-3CDSENST00000482273ENST00000535441HLCSchr21

38269160

-GRIK1chr21

30971257

-
intron-3CDSENST00000482273ENST00000389124HLCSchr21

38269160

-GRIK1chr21

30971257

-
intron-3CDSENST00000482273ENST00000399907HLCSchr21

38269160

-GRIK1chr21

30971257

-
intron-3CDSENST00000482273ENST00000399909HLCSchr21

38269160

-GRIK1chr21

30971257

-
intron-3CDSENST00000482273ENST00000309434HLCSchr21

38269160

-GRIK1chr21

30971257

-
intron-5UTRENST00000482273ENST00000472429HLCSchr21

38269160

-GRIK1chr21

30971257

-

check buttonORFfinder result based on the fusion transcript sequence of in-frame fusion genes.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandSeq length
(transcript)		BP loci
(transcript)		Predicted start
(transcript)		Predicted stop
(transcript)		Seq length
(amino acids)
ENST00000336648HLCSchr2138269160-ENST00000327783GRIK1chr2130971257-3971192217753673632
ENST00000336648HLCSchr2138269160-ENST00000389125GRIK1chr2130971257-3839192217753541588
ENST00000336648HLCSchr2138269160-ENST00000399913GRIK1chr2130971257-3884192217753586603
ENST00000336648HLCSchr2138269160-ENST00000399914GRIK1chr2130971257-3926192217753628617
ENST00000336648HLCSchr2138269160-ENST00000535441GRIK1chr2130971257-3587192217753586604
ENST00000336648HLCSchr2138269160-ENST00000389124GRIK1chr2130971257-3504192217753439554
ENST00000336648HLCSchr2138269160-ENST00000399907GRIK1chr2130971257-3884192217753580601
ENST00000336648HLCSchr2138269160-ENST00000399909GRIK1chr2130971257-3839192217753535586
ENST00000336648HLCSchr2138269160-ENST00000309434GRIK1chr2130971257-3581192217753580602

check buttonDeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandNo-coding scoreCoding score
ENST00000336648ENST00000327783HLCSchr2138269160-GRIK1chr2130971257-0.0010224460.99897754
ENST00000336648ENST00000389125HLCSchr2138269160-GRIK1chr2130971257-0.0010214660.9989786
ENST00000336648ENST00000399913HLCSchr2138269160-GRIK1chr2130971257-0.0009949060.99900514
ENST00000336648ENST00000399914HLCSchr2138269160-GRIK1chr2130971257-0.0010646530.9989354
ENST00000336648ENST00000535441HLCSchr2138269160-GRIK1chr2130971257-0.0016922620.9983077
ENST00000336648ENST00000389124HLCSchr2138269160-GRIK1chr2130971257-0.0016133170.99838674
ENST00000336648ENST00000399907HLCSchr2138269160-GRIK1chr2130971257-0.0009007510.99909925
ENST00000336648ENST00000399909HLCSchr2138269160-GRIK1chr2130971257-0.0009378580.9990621
ENST00000336648ENST00000309434HLCSchr2138269160-GRIK1chr2130971257-0.0013276490.99867237

Top

Fusion Genomic Features for HLCS-GRIK1


check buttonFusionAI prediction of the potential fusion gene breakpoint based on the pre-mature RNA sequence context (+/- 5kb of individual partner genes, total 20kb length sequence). FusionAI is a fusion gene breakpoint classifier based on convolutional neural network by comparing the fusion positive and negative sequence context of ~ 20K fusion gene data. From here, we can have the relative potentency of the 20K genomic sequence how individual sequnce will be likely used as the gene fusion breakpoints.
HgeneHchrHbpHstrandTgeneTchrTbpTstrand1-pp (fusion gene breakpoint)

check buttonDistribution of 44 human genomic features loci across 20kb length fusion breakpoint regions. We integrated a total of 44 different types of human genomic feature loci information across five big categories including virus integration sites, repeats, structural variants, chromatin states, and gene expression regulation. More details are in help page.

check buttonDistribution of 44 human genomic features loci across 20kb length fusion breakpoint regions that are ovelapped with the top 1% feature importance score regions. More details are in help page.

Top

Fusion Protein Features for HLCS-GRIK1


check button Go to

FGviewer for the breakpoints of chr21:38269160-chr21:30971257

.
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
FGviewer

check buttonMain function of each fusion partner protein. (from UniProt)
HgeneTgene
.GRIK1

P39086

FUNCTION: Might normally function as a transcriptional repressor. EWS-fusion-proteins (EFPS) may play a role in the tumorigenic process. They may disturb gene expression by mimicking, or interfering with the normal function of CTD-POLII within the transcription initiation complex. They may also contribute to an aberrant activation of the fusion protein target genes.FUNCTION: Ionotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist. May be involved in the transmission of light information from the retina to the hypothalamus.

check buttonRetention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at

download page


* Minus value of BPloci means that the break pointn is located before the CDS.
- In-frame and retained protein feature among the 13 regional features.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note
TgeneGRIK1chr21:38269160chr21:30971257ENST00000389125616531_533366.0906.0RegionGlutamate binding
TgeneGRIK1chr21:38269160chr21:30971257ENST00000389125616704_705366.0906.0RegionGlutamate binding
TgeneGRIK1chr21:38269160chr21:30971257ENST00000399907617531_533366.0919.0RegionGlutamate binding
TgeneGRIK1chr21:38269160chr21:30971257ENST00000399907617704_705366.0919.0RegionGlutamate binding
TgeneGRIK1chr21:38269160chr21:30971257ENST00000389125616598_653366.0906.0Topological domainCytoplasmic
TgeneGRIK1chr21:38269160chr21:30971257ENST00000389125616675_834366.0906.0Topological domainExtracellular
TgeneGRIK1chr21:38269160chr21:30971257ENST00000389125616856_918366.0906.0Topological domainCytoplasmic
TgeneGRIK1chr21:38269160chr21:30971257ENST00000399907617598_653366.0919.0Topological domainCytoplasmic
TgeneGRIK1chr21:38269160chr21:30971257ENST00000399907617675_834366.0919.0Topological domainExtracellular
TgeneGRIK1chr21:38269160chr21:30971257ENST00000399907617856_918366.0919.0Topological domainCytoplasmic
TgeneGRIK1chr21:38269160chr21:30971257ENST00000389125616577_597366.0906.0TransmembraneHelical
TgeneGRIK1chr21:38269160chr21:30971257ENST00000389125616654_674366.0906.0TransmembraneHelical
TgeneGRIK1chr21:38269160chr21:30971257ENST00000389125616835_855366.0906.0TransmembraneHelical
TgeneGRIK1chr21:38269160chr21:30971257ENST00000399907617577_597366.0919.0TransmembraneHelical
TgeneGRIK1chr21:38269160chr21:30971257ENST00000399907617654_674366.0919.0TransmembraneHelical
TgeneGRIK1chr21:38269160chr21:30971257ENST00000399907617835_855366.0919.0TransmembraneHelical

- In-frame and not-retained protein feature among the 13 regional features.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note
HgeneHLCSchr21:38269160chr21:30971257ENST00000336648-712463_652483.6666666666667727.0DomainBPL/LPL catalytic
HgeneHLCSchr21:38269160chr21:30971257ENST00000399120-712463_652483.6666666666667727.0DomainBPL/LPL catalytic
TgeneGRIK1chr21:38269160chr21:30971257ENST0000038912561631_576366.0906.0Topological domainExtracellular
TgeneGRIK1chr21:38269160chr21:30971257ENST0000039990761731_576366.0919.0Topological domainExtracellular


Top

Fusion Gene Sequence for HLCS-GRIK1


check button For in-frame fusion transcripts, we provide the fusion transcript sequences and fusion amino acid sequences. To have fusion amino acid sequence, we ran ORFfinder and chose the longest ORF among the all predicted ones.
>In-frame_ENST00000336648_ENST00000327783_TCGA-50-5944-01A_HLCS_chr21_38269160_-_GRIK1_chr21_30971257_length(transcript)=3971nt_BP=1922nt
ACGAGAGGGCGCAGCGCGGCGCGGCAGGGATTCGCGGGCGACCACCCGGCGCAGGAGCGGCCGCGTTTCGGCCTCAGAATCCATTGAAGA
CTTGAACAAGTGGGCCCTATTTCTTGTGTCTCCTTTTATACTTGAAGCAGAACACATAGCATTTGTGACGGAGAGCATTTGGGTACAAAG
TGAGAATTTACAGAGATCATCCTCTTCAGAAACAGTTCGGTTTTTGCCCACTAGGGATGATGTGGTTTCTCATGAGGTTACTTGCTCTAA
AGGACTTTATATTTTGGAACCATAAGAGCACCCTTGTGGCCCAGGCACTTTATGGATGATCCCTTTTAGTGCTCCCAGTAACCTTCCAAG
ATTGTCAAGTGGTCAGACTGTTGTTTGCCATTAGCTTGCAGACCTGGGGATCCTTATCGGCTAATTGCTGAAGCAAGTGTGGACAACTTC
AGCAAGCTGGGGGTGGCGTTCATGGAAGATAGACTCCACATGGATAATGGACTGGTACCCCAAAAGATTGTGTCGGTGCACTTGCAGGAC
TCCACTCTGAAGGAAGTTAAGGATCAGGTCTCAAACAAGCAAGCCCAGATCCTAGAGCCGAAGCCTGAACCTTCTCTTGAGATTAAGCCT
GAGCAGGACGGTATGGAGCATGTTGGCAGAGATGACCCAAAGGCTCTTGGTGAAGAACCCAAACAAAGGAGAGGCAGTGCCTCTGGGAGT
GAGCCTGCTGGGGACAGTGACAGGGGAGGGGGCCCCGTTGAGCATTATCACCTCCATCTGTCTAGTTGCCACGAGTGTCTGGAACTTGAG
AACAGCACCATTGAGTCAGTCAAGTTTGCGTCTGCCGAGAACATTCCAGACCTTCCCTACGATTATAGCAGCAGTTTGGAGAGTGTTGCT
GATGAGACCTCCCCCGAAAGAGAAGGGAGGAGAGTCAACCTCACGGGAAAGGCACCCAACATCCTCCTCTATGTGGGCTCCGACTCCCAG
GAAGCCCTCGGCCGGTTCCACGAGGTCCGGTCTGTGCTGGCCGACTGTGTGGACATTGACAGTTATATTCTCTACCACCTGCTGGAGGAC
AGTGCTCTCAGAGACCCGTGGACGGACAACTGTCTGCTGTTGGTCATTGCTACCAGGGAGTCCATTCCCGAAGACCTGTACCAGAAGTTC
ATGGCCTATCTTTCTCAGGGAGGGAAGGTGTTGGGCCTGTCTTCATCCTTCACCTTTGGTGGCTTTCAGGTGACAAGCAAGGGTGCACTG
CACAAGACAGTCCAGAACTTGGTTTTCTCCAAGGCTGACCAGAGCGAGGTGAAGCTCAGCGTCTTGAGCAGTGGCTGCAGGTACCAGGAA
GGCCCCGTCCGGCTCAGCCCCGGCAGGCTCCAGGGCCACCTGGAGAATGAGGACAAGGACAGGATGATTGTGCATGTGCCTTTTGGAACT
CGCGGGGGAGAAGCTGTTCTTTGCCAGGTGCACTTAGAACTACCTCCCAGCTCCAACATAGTGCAAACTCCAGAAGATTTTAACTTGCTC
AAGTCAAGCAATTTTAGAAGATACGAAGTCCTTAGAGAGATTCTGACAACCCTTGGCCTCAGCTGTGACATGAAACAAGTTCCTGCCTTA
ACTCCTCTTTACTTGCTGTCAGCTGCGGAGGAAATCAGGGATCCTCTTATGCAGTGGCTTGGGAAACATGTGGACTCCGAGGGAGAAATA
AAATCCGGCCAGCTCTCTCTTAGATTTGTTTCATCCTACGTGTCTGAAGTAGAAATAACCCCATCTTGTATACCTGTGGTGACCAACATG
GAGGCCTTCTCATCAGAACATTTCAACTTAGAGATCTATCGCCAAAATCTGCAGACCAAGCAGTTGGGGAAAGTAATTTTGTTTGCCGAA
GTGACCCCCACAACGATGCGTCTCCTGGATGGGCCCGGTGGGATGGCTTGACTGGGCATATCACCTTTAATAAAACCAATGGCTTGAGGA
AGGATTTTGATCTGGACATTATTAGTCTCAAAGAGGAAGGAACTGAAAAGGCTGCTGGCGAAGTGTCTAAACACTTGTATAAAGTGTGGA
AGAAGATTGGGATTTGGAATTCCAACAGTGGGCTTAACATGACGGACAGCAACAAAGACAAGTCCAGCAATATCACTGATTCATTGGCCA
ACAGAACACTCATTGTCACCACCATTCTGGAAGAACCCTATGTTATGTACAGGAAATCTGATAAGCCTCTATATGGAAATGACAGATTTG
AAGGATATTGCCTAGACCTGTTGAAAGAATTGTCAAACATCCTGGGTTTCATTTATGATGTTAAACTAGTTCCCGATGGCAAATATGGGG
CCCAGAATGACAAAGGGGAGTGGAACGGGATGGTTAAAGAACTCATAGATCACAGGGCTGACCTGGCAGTGGCTCCTCTTACCATCACCT
ACGTGCGGGAGAAAGTCATTGACTTCTCCAAACCCTTCATGACCCTAGGCATCAGCATTCTCTACCGGAAGCCCAATGGTACCAATCCAG
GCGTTTTCTCCTTCCTCAACCCCCTGTCTCCAGATATTTGGATGTATGTGCTCTTAGCCTGCTTGGGAGTCAGCTGTGTACTCTTTGTGA
TTGCAAGGTTTACACCCTACGAGTGGTATAACCCCCACCCATGCAACCCTGACTCAGACGTGGTGGAAAACAATTTTACTTTACTAAATA
GTTTCTGGTTTGGAGTTGGAGCTCTCATGCAGCAAGGATCAGAGCTGATGCCCAAAGCTCTATCGACCAGAATAGTTGGAGGGATATGGT
GGTTTTTCACCCTAATCATCATTTCATCCTACACGGCCAATCTGGCTGCCTTCTTGACAGTAGAGAGAATGGAATCCCCCATAGATTCGG
CAGATGATCTGGCAAAGCAAACCAAGATAGAATATGGGGCGGTTAGAGATGGATCAACAATGACCTTCTTCAAGAAATCAAAAATCTCCA
CCTATGAGAAGATGTGGGCTTTCATGAGCAGCAGGCAGCAGACCGCCCTGGTAAGAAACAGTGATGAGGGGATCCAGAGAGTGCTCACCA
CAGACTACGCGCTGCTGATGGAGTCCACCAGCATTGAGTATGTGACGCAGAGAAACTGCAACCTCACTCAGATCGGGGGCCTCATTGACT
CCAAAGGTTACGGAGTGGGAACACCTATTGGTTCTCCTTACCGGGATAAAATTACTATTGCTATTCTTCAACTCCAAGAAGAAGGGAAGC
TGCATATGATGAAAGAGAAGTGGTGGCGTGGGAATGGCTGCCCCGAGGAAGACAACAAAGAAGCCAGTGCCCTGGGAGTGGAAAATATTG
GAGGCATCTTCATTGTTCTGGCTGCCGGACTGGTCCTTTCTGTATTTGTAGCTATTGGAGAATTCATATACAAATCACGGAAGAATAATG
ATATTGAACAGAAAGGAAAGTCATCAAGAATTAGATTTTATTTTAGGAACAAAGTAAGGTTTCATGGGAGAAAAAAACAAAGCCTTGGTG
TAGAGAAGTGTCTCTCTTTCAACGCTATCATGGAAGAACTGGGAATCTCACTGAAGAATCAGAAAAAAATAAAGAAAAAGTCAAGAACTA
AGGGGAAATCTTCCTTCACAAGTATCCTTACTTGTCATCAGAGACGAACTCAGAGAAAAGAGACTGTGGCGTGATCCAAGGAAACGCCTG
TAGGAAGAAAAAGGATGCATTCCCTACAGATTTTTGGAGAAAGGATTTCTGAGGAGTGTGTGATGTGTTTCCATATATCTATATCCATAA
CTCTGATTATGAATACAGATATAAGAAATACAAAAGTTTAAAAAGCTCACATAGATATGACTTGGGAAGTGACACCAGTTCTTTTAAAAT
AAATTTGTATGCTCAATTATTTTGCATTTTTTCTTTTTCCCCAATAAATTGTTGTGTGTGCTTTCTAAATAATAATAAAACAACAGGGTT

>In-frame_ENST00000336648_ENST00000327783_TCGA-50-5944-01A_HLCS_chr21_38269160_-_GRIK1_chr21_30971257_length(amino acids)=632AA_start in transcript=1775_stop in transcript=3673
MYTCGDQHGGLLIRTFQLRDLSPKSADQAVGESNFVCRSDPHNDASPGWARWDGLTGHITFNKTNGLRKDFDLDIISLKEEGTEKAAGEV
SKHLYKVWKKIGIWNSNSGLNMTDSNKDKSSNITDSLANRTLIVTTILEEPYVMYRKSDKPLYGNDRFEGYCLDLLKELSNILGFIYDVK
LVPDGKYGAQNDKGEWNGMVKELIDHRADLAVAPLTITYVREKVIDFSKPFMTLGISILYRKPNGTNPGVFSFLNPLSPDIWMYVLLACL
GVSCVLFVIARFTPYEWYNPHPCNPDSDVVENNFTLLNSFWFGVGALMQQGSELMPKALSTRIVGGIWWFFTLIIISSYTANLAAFLTVE
RMESPIDSADDLAKQTKIEYGAVRDGSTMTFFKKSKISTYEKMWAFMSSRQQTALVRNSDEGIQRVLTTDYALLMESTSIEYVTQRNCNL
TQIGGLIDSKGYGVGTPIGSPYRDKITIAILQLQEEGKLHMMKEKWWRGNGCPEEDNKEASALGVENIGGIFIVLAAGLVLSVFVAIGEF
IYKSRKNNDIEQKGKSSRIRFYFRNKVRFHGRKKQSLGVEKCLSFNAIMEELGISLKNQKKIKKKSRTKGKSSFTSILTCHQRRTQRKET

--------------------------------------------------------------
>In-frame_ENST00000336648_ENST00000389125_TCGA-50-5944-01A_HLCS_chr21_38269160_-_GRIK1_chr21_30971257_length(transcript)=3839nt_BP=1922nt
ACGAGAGGGCGCAGCGCGGCGCGGCAGGGATTCGCGGGCGACCACCCGGCGCAGGAGCGGCCGCGTTTCGGCCTCAGAATCCATTGAAGA
CTTGAACAAGTGGGCCCTATTTCTTGTGTCTCCTTTTATACTTGAAGCAGAACACATAGCATTTGTGACGGAGAGCATTTGGGTACAAAG
TGAGAATTTACAGAGATCATCCTCTTCAGAAACAGTTCGGTTTTTGCCCACTAGGGATGATGTGGTTTCTCATGAGGTTACTTGCTCTAA
AGGACTTTATATTTTGGAACCATAAGAGCACCCTTGTGGCCCAGGCACTTTATGGATGATCCCTTTTAGTGCTCCCAGTAACCTTCCAAG
ATTGTCAAGTGGTCAGACTGTTGTTTGCCATTAGCTTGCAGACCTGGGGATCCTTATCGGCTAATTGCTGAAGCAAGTGTGGACAACTTC
AGCAAGCTGGGGGTGGCGTTCATGGAAGATAGACTCCACATGGATAATGGACTGGTACCCCAAAAGATTGTGTCGGTGCACTTGCAGGAC
TCCACTCTGAAGGAAGTTAAGGATCAGGTCTCAAACAAGCAAGCCCAGATCCTAGAGCCGAAGCCTGAACCTTCTCTTGAGATTAAGCCT
GAGCAGGACGGTATGGAGCATGTTGGCAGAGATGACCCAAAGGCTCTTGGTGAAGAACCCAAACAAAGGAGAGGCAGTGCCTCTGGGAGT
GAGCCTGCTGGGGACAGTGACAGGGGAGGGGGCCCCGTTGAGCATTATCACCTCCATCTGTCTAGTTGCCACGAGTGTCTGGAACTTGAG
AACAGCACCATTGAGTCAGTCAAGTTTGCGTCTGCCGAGAACATTCCAGACCTTCCCTACGATTATAGCAGCAGTTTGGAGAGTGTTGCT
GATGAGACCTCCCCCGAAAGAGAAGGGAGGAGAGTCAACCTCACGGGAAAGGCACCCAACATCCTCCTCTATGTGGGCTCCGACTCCCAG
GAAGCCCTCGGCCGGTTCCACGAGGTCCGGTCTGTGCTGGCCGACTGTGTGGACATTGACAGTTATATTCTCTACCACCTGCTGGAGGAC
AGTGCTCTCAGAGACCCGTGGACGGACAACTGTCTGCTGTTGGTCATTGCTACCAGGGAGTCCATTCCCGAAGACCTGTACCAGAAGTTC
ATGGCCTATCTTTCTCAGGGAGGGAAGGTGTTGGGCCTGTCTTCATCCTTCACCTTTGGTGGCTTTCAGGTGACAAGCAAGGGTGCACTG
CACAAGACAGTCCAGAACTTGGTTTTCTCCAAGGCTGACCAGAGCGAGGTGAAGCTCAGCGTCTTGAGCAGTGGCTGCAGGTACCAGGAA
GGCCCCGTCCGGCTCAGCCCCGGCAGGCTCCAGGGCCACCTGGAGAATGAGGACAAGGACAGGATGATTGTGCATGTGCCTTTTGGAACT
CGCGGGGGAGAAGCTGTTCTTTGCCAGGTGCACTTAGAACTACCTCCCAGCTCCAACATAGTGCAAACTCCAGAAGATTTTAACTTGCTC
AAGTCAAGCAATTTTAGAAGATACGAAGTCCTTAGAGAGATTCTGACAACCCTTGGCCTCAGCTGTGACATGAAACAAGTTCCTGCCTTA
ACTCCTCTTTACTTGCTGTCAGCTGCGGAGGAAATCAGGGATCCTCTTATGCAGTGGCTTGGGAAACATGTGGACTCCGAGGGAGAAATA
AAATCCGGCCAGCTCTCTCTTAGATTTGTTTCATCCTACGTGTCTGAAGTAGAAATAACCCCATCTTGTATACCTGTGGTGACCAACATG
GAGGCCTTCTCATCAGAACATTTCAACTTAGAGATCTATCGCCAAAATCTGCAGACCAAGCAGTTGGGGAAAGTAATTTTGTTTGCCGAA
GTGACCCCCACAACGATGCGTCTCCTGGATGGGCCCGGTGGGATGGCTTGACTGGGCATATCACCTTTAATAAAACCAATGGCTTGAGGA
AGGATTTTGATCTGGACATTATTAGTCTCAAAGAGGAAGGAACTGAAAAGATTGGGATTTGGAATTCCAACAGTGGGCTTAACATGACGG
ACAGCAACAAAGACAAGTCCAGCAATATCACTGATTCATTGGCCAACAGAACACTCATTGTCACCACCATTCTGGAAGAACCCTATGTTA
TGTACAGGAAATCTGATAAGCCTCTATATGGAAATGACAGATTTGAAGGATATTGCCTAGACCTGTTGAAAGAATTGTCAAACATCCTGG
GTTTCATTTATGATGTTAAACTAGTTCCCGATGGCAAATATGGGGCCCAGAATGACAAAGGGGAGTGGAACGGGATGGTTAAAGAACTCA
TAGATCACAGGGCTGACCTGGCAGTGGCTCCTCTTACCATCACCTACGTGCGGGAGAAAGTCATTGACTTCTCCAAACCCTTCATGACCC
TAGGCATCAGCATTCTCTACCGGAAGCCCAATGGTACCAATCCAGGCGTTTTCTCCTTCCTCAACCCCCTGTCTCCAGATATTTGGATGT
ATGTGCTCTTAGCCTGCTTGGGAGTCAGCTGTGTACTCTTTGTGATTGCAAGGTTTACACCCTACGAGTGGTATAACCCCCACCCATGCA
ACCCTGACTCAGACGTGGTGGAAAACAATTTTACTTTACTAAATAGTTTCTGGTTTGGAGTTGGAGCTCTCATGCAGCAAGGATCAGAGC
TGATGCCCAAAGCTCTATCGACCAGAATAGTTGGAGGGATATGGTGGTTTTTCACCCTAATCATCATTTCATCCTACACGGCCAATCTGG
CTGCCTTCTTGACAGTAGAGAGAATGGAATCCCCCATAGATTCGGCAGATGATCTGGCAAAGCAAACCAAGATAGAATATGGGGCGGTTA
GAGATGGATCAACAATGACCTTCTTCAAGAAATCAAAAATCTCCACCTATGAGAAGATGTGGGCTTTCATGAGCAGCAGGCAGCAGACCG
CCCTGGTAAGAAACAGTGATGAGGGGATCCAGAGAGTGCTCACCACAGACTACGCGCTGCTGATGGAGTCCACCAGCATTGAGTATGTGA
CGCAGAGAAACTGCAACCTCACTCAGATCGGGGGCCTCATTGACTCCAAAGGTTACGGAGTGGGAACACCTATTGGTTCTCCTTACCGGG
ATAAAATTACTATTGCTATTCTTCAACTCCAAGAAGAAGGGAAGCTGCATATGATGAAAGAGAAGTGGTGGCGTGGGAATGGCTGCCCCG
AGGAAGACAACAAAGAAGCCAGTGCCCTGGGAGTGGAAAATATTGGAGGCATCTTCATTGTTCTGGCTGCCGGACTGGTCCTTTCTGTAT
TTGTAGCTATTGGAGAATTCATATACAAATCACGGAAGAATAATGATATTGAACAGTGTCTCTCTTTCAACGCTATCATGGAAGAACTGG
GAATCTCACTGAAGAATCAGAAAAAAATAAAGAAAAAGTCAAGAACTAAGGGGAAATCTTCCTTCACAAGTATCCTTACTTGTCATCAGA
GACGAACTCAGAGAAAAGAGACTGTGGCGTGATCCAAGGAAACGCCTGTAGGAAGAAAAAGGATGCATTCCCTACAGATTTTTGGAGAAA
GGATTTCTGAGGAGTGTGTGATGTGTTTCCATATATCTATATCCATAACTCTGATTATGAATACAGATATAAGAAATACAAAAGTTTAAA
AAGCTCACATAGATATGACTTGGGAAGTGACACCAGTTCTTTTAAAATAAATTTGTATGCTCAATTATTTTGCATTTTTTCTTTTTCCCC

>In-frame_ENST00000336648_ENST00000389125_TCGA-50-5944-01A_HLCS_chr21_38269160_-_GRIK1_chr21_30971257_length(amino acids)=588AA_start in transcript=1775_stop in transcript=3541
MYTCGDQHGGLLIRTFQLRDLSPKSADQAVGESNFVCRSDPHNDASPGWARWDGLTGHITFNKTNGLRKDFDLDIISLKEEGTEKIGIWN
SNSGLNMTDSNKDKSSNITDSLANRTLIVTTILEEPYVMYRKSDKPLYGNDRFEGYCLDLLKELSNILGFIYDVKLVPDGKYGAQNDKGE
WNGMVKELIDHRADLAVAPLTITYVREKVIDFSKPFMTLGISILYRKPNGTNPGVFSFLNPLSPDIWMYVLLACLGVSCVLFVIARFTPY
EWYNPHPCNPDSDVVENNFTLLNSFWFGVGALMQQGSELMPKALSTRIVGGIWWFFTLIIISSYTANLAAFLTVERMESPIDSADDLAKQ
TKIEYGAVRDGSTMTFFKKSKISTYEKMWAFMSSRQQTALVRNSDEGIQRVLTTDYALLMESTSIEYVTQRNCNLTQIGGLIDSKGYGVG
TPIGSPYRDKITIAILQLQEEGKLHMMKEKWWRGNGCPEEDNKEASALGVENIGGIFIVLAAGLVLSVFVAIGEFIYKSRKNNDIEQCLS

--------------------------------------------------------------
>In-frame_ENST00000336648_ENST00000399913_TCGA-50-5944-01A_HLCS_chr21_38269160_-_GRIK1_chr21_30971257_length(transcript)=3884nt_BP=1922nt
ACGAGAGGGCGCAGCGCGGCGCGGCAGGGATTCGCGGGCGACCACCCGGCGCAGGAGCGGCCGCGTTTCGGCCTCAGAATCCATTGAAGA
CTTGAACAAGTGGGCCCTATTTCTTGTGTCTCCTTTTATACTTGAAGCAGAACACATAGCATTTGTGACGGAGAGCATTTGGGTACAAAG
TGAGAATTTACAGAGATCATCCTCTTCAGAAACAGTTCGGTTTTTGCCCACTAGGGATGATGTGGTTTCTCATGAGGTTACTTGCTCTAA
AGGACTTTATATTTTGGAACCATAAGAGCACCCTTGTGGCCCAGGCACTTTATGGATGATCCCTTTTAGTGCTCCCAGTAACCTTCCAAG
ATTGTCAAGTGGTCAGACTGTTGTTTGCCATTAGCTTGCAGACCTGGGGATCCTTATCGGCTAATTGCTGAAGCAAGTGTGGACAACTTC
AGCAAGCTGGGGGTGGCGTTCATGGAAGATAGACTCCACATGGATAATGGACTGGTACCCCAAAAGATTGTGTCGGTGCACTTGCAGGAC
TCCACTCTGAAGGAAGTTAAGGATCAGGTCTCAAACAAGCAAGCCCAGATCCTAGAGCCGAAGCCTGAACCTTCTCTTGAGATTAAGCCT
GAGCAGGACGGTATGGAGCATGTTGGCAGAGATGACCCAAAGGCTCTTGGTGAAGAACCCAAACAAAGGAGAGGCAGTGCCTCTGGGAGT
GAGCCTGCTGGGGACAGTGACAGGGGAGGGGGCCCCGTTGAGCATTATCACCTCCATCTGTCTAGTTGCCACGAGTGTCTGGAACTTGAG
AACAGCACCATTGAGTCAGTCAAGTTTGCGTCTGCCGAGAACATTCCAGACCTTCCCTACGATTATAGCAGCAGTTTGGAGAGTGTTGCT
GATGAGACCTCCCCCGAAAGAGAAGGGAGGAGAGTCAACCTCACGGGAAAGGCACCCAACATCCTCCTCTATGTGGGCTCCGACTCCCAG
GAAGCCCTCGGCCGGTTCCACGAGGTCCGGTCTGTGCTGGCCGACTGTGTGGACATTGACAGTTATATTCTCTACCACCTGCTGGAGGAC
AGTGCTCTCAGAGACCCGTGGACGGACAACTGTCTGCTGTTGGTCATTGCTACCAGGGAGTCCATTCCCGAAGACCTGTACCAGAAGTTC
ATGGCCTATCTTTCTCAGGGAGGGAAGGTGTTGGGCCTGTCTTCATCCTTCACCTTTGGTGGCTTTCAGGTGACAAGCAAGGGTGCACTG
CACAAGACAGTCCAGAACTTGGTTTTCTCCAAGGCTGACCAGAGCGAGGTGAAGCTCAGCGTCTTGAGCAGTGGCTGCAGGTACCAGGAA
GGCCCCGTCCGGCTCAGCCCCGGCAGGCTCCAGGGCCACCTGGAGAATGAGGACAAGGACAGGATGATTGTGCATGTGCCTTTTGGAACT
CGCGGGGGAGAAGCTGTTCTTTGCCAGGTGCACTTAGAACTACCTCCCAGCTCCAACATAGTGCAAACTCCAGAAGATTTTAACTTGCTC
AAGTCAAGCAATTTTAGAAGATACGAAGTCCTTAGAGAGATTCTGACAACCCTTGGCCTCAGCTGTGACATGAAACAAGTTCCTGCCTTA
ACTCCTCTTTACTTGCTGTCAGCTGCGGAGGAAATCAGGGATCCTCTTATGCAGTGGCTTGGGAAACATGTGGACTCCGAGGGAGAAATA
AAATCCGGCCAGCTCTCTCTTAGATTTGTTTCATCCTACGTGTCTGAAGTAGAAATAACCCCATCTTGTATACCTGTGGTGACCAACATG
GAGGCCTTCTCATCAGAACATTTCAACTTAGAGATCTATCGCCAAAATCTGCAGACCAAGCAGTTGGGGAAAGTAATTTTGTTTGCCGAA
GTGACCCCCACAACGATGCGTCTCCTGGATGGGCCCGGTGGGATGGCTTGACTGGGCATATCACCTTTAATAAAACCAATGGCTTGAGGA
AGGATTTTGATCTGGACATTATTAGTCTCAAAGAGGAAGGAACTGAAAAGGCTGCTGGCGAAGTGTCTAAACACTTGTATAAAGTGTGGA
AGAAGATTGGGATTTGGAATTCCAACAGTGGGCTTAACATGACGGACAGCAACAAAGACAAGTCCAGCAATATCACTGATTCATTGGCCA
ACAGAACACTCATTGTCACCACCATTCTGGAAGAACCCTATGTTATGTACAGGAAATCTGATAAGCCTCTATATGGAAATGACAGATTTG
AAGGATATTGCCTAGACCTGTTGAAAGAATTGTCAAACATCCTGGGTTTCATTTATGATGTTAAACTAGTTCCCGATGGCAAATATGGGG
CCCAGAATGACAAAGGGGAGTGGAACGGGATGGTTAAAGAACTCATAGATCACAGGGCTGACCTGGCAGTGGCTCCTCTTACCATCACCT
ACGTGCGGGAGAAAGTCATTGACTTCTCCAAACCCTTCATGACCCTAGGCATCAGCATTCTCTACCGGAAGCCCAATGGTACCAATCCAG
GCGTTTTCTCCTTCCTCAACCCCCTGTCTCCAGATATTTGGATGTATGTGCTCTTAGCCTGCTTGGGAGTCAGCTGTGTACTCTTTGTGA
TTGCAAGGTTTACACCCTACGAGTGGTATAACCCCCACCCATGCAACCCTGACTCAGACGTGGTGGAAAACAATTTTACTTTACTAAATA
GTTTCTGGTTTGGAGTTGGAGCTCTCATGCAGCAAGGATCAGAGCTGATGCCCAAAGCTCTATCGACCAGAATAGTTGGAGGGATATGGT
GGTTTTTCACCCTAATCATCATTTCATCCTACACGGCCAATCTGGCTGCCTTCTTGACAGTAGAGAGAATGGAATCCCCCATAGATTCGG
CAGATGATCTGGCAAAGCAAACCAAGATAGAATATGGGGCGGTTAGAGATGGATCAACAATGACCTTCTTCAAGAAATCAAAAATCTCCA
CCTATGAGAAGATGTGGGCTTTCATGAGCAGCAGGCAGCAGACCGCCCTGGTAAGAAACAGTGATGAGGGGATCCAGAGAGTGCTCACCA
CAGACTACGCGCTGCTGATGGAGTCCACCAGCATTGAGTATGTGACGCAGAGAAACTGCAACCTCACTCAGATCGGGGGCCTCATTGACT
CCAAAGGTTACGGAGTGGGAACACCTATTGGTTCTCCTTACCGGGATAAAATTACTATTGCTATTCTTCAACTCCAAGAAGAAGGGAAGC
TGCATATGATGAAAGAGAAGTGGTGGCGTGGGAATGGCTGCCCCGAGGAAGACAACAAAGAAGCCAGTGCCCTGGGAGTGGAAAATATTG
GAGGCATCTTCATTGTTCTGGCTGCCGGACTGGTCCTTTCTGTATTTGTAGCTATTGGAGAATTCATATACAAATCACGGAAGAATAATG
ATATTGAACAGTGTCTCTCTTTCAACGCTATCATGGAAGAACTGGGAATCTCACTGAAGAATCAGAAAAAAATAAAGAAAAAGTCAAGAA
CTAAGGGGAAATCTTCCTTCACAAGTATCCTTACTTGTCATCAGAGACGAACTCAGAGAAAAGAGACTGTGGCGTGATCCAAGGAAACGC
CTGTAGGAAGAAAAAGGATGCATTCCCTACAGATTTTTGGAGAAAGGATTTCTGAGGAGTGTGTGATGTGTTTCCATATATCTATATCCA
TAACTCTGATTATGAATACAGATATAAGAAATACAAAAGTTTAAAAAGCTCACATAGATATGACTTGGGAAGTGACACCAGTTCTTTTAA
AATAAATTTGTATGCTCAATTATTTTGCATTTTTTCTTTTTCCCCAATAAATTGTTGTGTGTGCTTTCTAAATAATAATAAAACAACAGG

>In-frame_ENST00000336648_ENST00000399913_TCGA-50-5944-01A_HLCS_chr21_38269160_-_GRIK1_chr21_30971257_length(amino acids)=603AA_start in transcript=1775_stop in transcript=3586
MYTCGDQHGGLLIRTFQLRDLSPKSADQAVGESNFVCRSDPHNDASPGWARWDGLTGHITFNKTNGLRKDFDLDIISLKEEGTEKAAGEV
SKHLYKVWKKIGIWNSNSGLNMTDSNKDKSSNITDSLANRTLIVTTILEEPYVMYRKSDKPLYGNDRFEGYCLDLLKELSNILGFIYDVK
LVPDGKYGAQNDKGEWNGMVKELIDHRADLAVAPLTITYVREKVIDFSKPFMTLGISILYRKPNGTNPGVFSFLNPLSPDIWMYVLLACL
GVSCVLFVIARFTPYEWYNPHPCNPDSDVVENNFTLLNSFWFGVGALMQQGSELMPKALSTRIVGGIWWFFTLIIISSYTANLAAFLTVE
RMESPIDSADDLAKQTKIEYGAVRDGSTMTFFKKSKISTYEKMWAFMSSRQQTALVRNSDEGIQRVLTTDYALLMESTSIEYVTQRNCNL
TQIGGLIDSKGYGVGTPIGSPYRDKITIAILQLQEEGKLHMMKEKWWRGNGCPEEDNKEASALGVENIGGIFIVLAAGLVLSVFVAIGEF

--------------------------------------------------------------
>In-frame_ENST00000336648_ENST00000399914_TCGA-50-5944-01A_HLCS_chr21_38269160_-_GRIK1_chr21_30971257_length(transcript)=3926nt_BP=1922nt
ACGAGAGGGCGCAGCGCGGCGCGGCAGGGATTCGCGGGCGACCACCCGGCGCAGGAGCGGCCGCGTTTCGGCCTCAGAATCCATTGAAGA
CTTGAACAAGTGGGCCCTATTTCTTGTGTCTCCTTTTATACTTGAAGCAGAACACATAGCATTTGTGACGGAGAGCATTTGGGTACAAAG
TGAGAATTTACAGAGATCATCCTCTTCAGAAACAGTTCGGTTTTTGCCCACTAGGGATGATGTGGTTTCTCATGAGGTTACTTGCTCTAA
AGGACTTTATATTTTGGAACCATAAGAGCACCCTTGTGGCCCAGGCACTTTATGGATGATCCCTTTTAGTGCTCCCAGTAACCTTCCAAG
ATTGTCAAGTGGTCAGACTGTTGTTTGCCATTAGCTTGCAGACCTGGGGATCCTTATCGGCTAATTGCTGAAGCAAGTGTGGACAACTTC
AGCAAGCTGGGGGTGGCGTTCATGGAAGATAGACTCCACATGGATAATGGACTGGTACCCCAAAAGATTGTGTCGGTGCACTTGCAGGAC
TCCACTCTGAAGGAAGTTAAGGATCAGGTCTCAAACAAGCAAGCCCAGATCCTAGAGCCGAAGCCTGAACCTTCTCTTGAGATTAAGCCT
GAGCAGGACGGTATGGAGCATGTTGGCAGAGATGACCCAAAGGCTCTTGGTGAAGAACCCAAACAAAGGAGAGGCAGTGCCTCTGGGAGT
GAGCCTGCTGGGGACAGTGACAGGGGAGGGGGCCCCGTTGAGCATTATCACCTCCATCTGTCTAGTTGCCACGAGTGTCTGGAACTTGAG
AACAGCACCATTGAGTCAGTCAAGTTTGCGTCTGCCGAGAACATTCCAGACCTTCCCTACGATTATAGCAGCAGTTTGGAGAGTGTTGCT
GATGAGACCTCCCCCGAAAGAGAAGGGAGGAGAGTCAACCTCACGGGAAAGGCACCCAACATCCTCCTCTATGTGGGCTCCGACTCCCAG
GAAGCCCTCGGCCGGTTCCACGAGGTCCGGTCTGTGCTGGCCGACTGTGTGGACATTGACAGTTATATTCTCTACCACCTGCTGGAGGAC
AGTGCTCTCAGAGACCCGTGGACGGACAACTGTCTGCTGTTGGTCATTGCTACCAGGGAGTCCATTCCCGAAGACCTGTACCAGAAGTTC
ATGGCCTATCTTTCTCAGGGAGGGAAGGTGTTGGGCCTGTCTTCATCCTTCACCTTTGGTGGCTTTCAGGTGACAAGCAAGGGTGCACTG
CACAAGACAGTCCAGAACTTGGTTTTCTCCAAGGCTGACCAGAGCGAGGTGAAGCTCAGCGTCTTGAGCAGTGGCTGCAGGTACCAGGAA
GGCCCCGTCCGGCTCAGCCCCGGCAGGCTCCAGGGCCACCTGGAGAATGAGGACAAGGACAGGATGATTGTGCATGTGCCTTTTGGAACT
CGCGGGGGAGAAGCTGTTCTTTGCCAGGTGCACTTAGAACTACCTCCCAGCTCCAACATAGTGCAAACTCCAGAAGATTTTAACTTGCTC
AAGTCAAGCAATTTTAGAAGATACGAAGTCCTTAGAGAGATTCTGACAACCCTTGGCCTCAGCTGTGACATGAAACAAGTTCCTGCCTTA
ACTCCTCTTTACTTGCTGTCAGCTGCGGAGGAAATCAGGGATCCTCTTATGCAGTGGCTTGGGAAACATGTGGACTCCGAGGGAGAAATA
AAATCCGGCCAGCTCTCTCTTAGATTTGTTTCATCCTACGTGTCTGAAGTAGAAATAACCCCATCTTGTATACCTGTGGTGACCAACATG
GAGGCCTTCTCATCAGAACATTTCAACTTAGAGATCTATCGCCAAAATCTGCAGACCAAGCAGTTGGGGAAAGTAATTTTGTTTGCCGAA
GTGACCCCCACAACGATGCGTCTCCTGGATGGGCCCGGTGGGATGGCTTGACTGGGCATATCACCTTTAATAAAACCAATGGCTTGAGGA
AGGATTTTGATCTGGACATTATTAGTCTCAAAGAGGAAGGAACTGAAAAGATTGGGATTTGGAATTCCAACAGTGGGCTTAACATGACGG
ACAGCAACAAAGACAAGTCCAGCAATATCACTGATTCATTGGCCAACAGAACACTCATTGTCACCACCATTCTGGAAGAACCCTATGTTA
TGTACAGGAAATCTGATAAGCCTCTATATGGAAATGACAGATTTGAAGGATATTGCCTAGACCTGTTGAAAGAATTGTCAAACATCCTGG
GTTTCATTTATGATGTTAAACTAGTTCCCGATGGCAAATATGGGGCCCAGAATGACAAAGGGGAGTGGAACGGGATGGTTAAAGAACTCA
TAGATCACAGGGCTGACCTGGCAGTGGCTCCTCTTACCATCACCTACGTGCGGGAGAAAGTCATTGACTTCTCCAAACCCTTCATGACCC
TAGGCATCAGCATTCTCTACCGGAAGCCCAATGGTACCAATCCAGGCGTTTTCTCCTTCCTCAACCCCCTGTCTCCAGATATTTGGATGT
ATGTGCTCTTAGCCTGCTTGGGAGTCAGCTGTGTACTCTTTGTGATTGCAAGGTTTACACCCTACGAGTGGTATAACCCCCACCCATGCA
ACCCTGACTCAGACGTGGTGGAAAACAATTTTACTTTACTAAATAGTTTCTGGTTTGGAGTTGGAGCTCTCATGCAGCAAGGATCAGAGC
TGATGCCCAAAGCTCTATCGACCAGAATAGTTGGAGGGATATGGTGGTTTTTCACCCTAATCATCATTTCATCCTACACGGCCAATCTGG
CTGCCTTCTTGACAGTAGAGAGAATGGAATCCCCCATAGATTCGGCAGATGATCTGGCAAAGCAAACCAAGATAGAATATGGGGCGGTTA
GAGATGGATCAACAATGACCTTCTTCAAGAAATCAAAAATCTCCACCTATGAGAAGATGTGGGCTTTCATGAGCAGCAGGCAGCAGACCG
CCCTGGTAAGAAACAGTGATGAGGGGATCCAGAGAGTGCTCACCACAGACTACGCGCTGCTGATGGAGTCCACCAGCATTGAGTATGTGA
CGCAGAGAAACTGCAACCTCACTCAGATCGGGGGCCTCATTGACTCCAAAGGTTACGGAGTGGGAACACCTATTGGTTCTCCTTACCGGG
ATAAAATTACTATTGCTATTCTTCAACTCCAAGAAGAAGGGAAGCTGCATATGATGAAAGAGAAGTGGTGGCGTGGGAATGGCTGCCCCG
AGGAAGACAACAAAGAAGCCAGTGCCCTGGGAGTGGAAAATATTGGAGGCATCTTCATTGTTCTGGCTGCCGGACTGGTCCTTTCTGTAT
TTGTAGCTATTGGAGAATTCATATACAAATCACGGAAGAATAATGATATTGAACAGAAAGGAAAGTCATCAAGAATTAGATTTTATTTTA
GGAACAAAGTAAGGTTTCATGGGAGAAAAAAACAAAGCCTTGGTGTAGAGAAGTGTCTCTCTTTCAACGCTATCATGGAAGAACTGGGAA
TCTCACTGAAGAATCAGAAAAAAATAAAGAAAAAGTCAAGAACTAAGGGGAAATCTTCCTTCACAAGTATCCTTACTTGTCATCAGAGAC
GAACTCAGAGAAAAGAGACTGTGGCGTGATCCAAGGAAACGCCTGTAGGAAGAAAAAGGATGCATTCCCTACAGATTTTTGGAGAAAGGA
TTTCTGAGGAGTGTGTGATGTGTTTCCATATATCTATATCCATAACTCTGATTATGAATACAGATATAAGAAATACAAAAGTTTAAAAAG
CTCACATAGATATGACTTGGGAAGTGACACCAGTTCTTTTAAAATAAATTTGTATGCTCAATTATTTTGCATTTTTTCTTTTTCCCCAAT

>In-frame_ENST00000336648_ENST00000399914_TCGA-50-5944-01A_HLCS_chr21_38269160_-_GRIK1_chr21_30971257_length(amino acids)=617AA_start in transcript=1775_stop in transcript=3628
MYTCGDQHGGLLIRTFQLRDLSPKSADQAVGESNFVCRSDPHNDASPGWARWDGLTGHITFNKTNGLRKDFDLDIISLKEEGTEKIGIWN
SNSGLNMTDSNKDKSSNITDSLANRTLIVTTILEEPYVMYRKSDKPLYGNDRFEGYCLDLLKELSNILGFIYDVKLVPDGKYGAQNDKGE
WNGMVKELIDHRADLAVAPLTITYVREKVIDFSKPFMTLGISILYRKPNGTNPGVFSFLNPLSPDIWMYVLLACLGVSCVLFVIARFTPY
EWYNPHPCNPDSDVVENNFTLLNSFWFGVGALMQQGSELMPKALSTRIVGGIWWFFTLIIISSYTANLAAFLTVERMESPIDSADDLAKQ
TKIEYGAVRDGSTMTFFKKSKISTYEKMWAFMSSRQQTALVRNSDEGIQRVLTTDYALLMESTSIEYVTQRNCNLTQIGGLIDSKGYGVG
TPIGSPYRDKITIAILQLQEEGKLHMMKEKWWRGNGCPEEDNKEASALGVENIGGIFIVLAAGLVLSVFVAIGEFIYKSRKNNDIEQKGK

--------------------------------------------------------------
>In-frame_ENST00000336648_ENST00000535441_TCGA-50-5944-01A_HLCS_chr21_38269160_-_GRIK1_chr21_30971257_length(transcript)=3587nt_BP=1922nt
ACGAGAGGGCGCAGCGCGGCGCGGCAGGGATTCGCGGGCGACCACCCGGCGCAGGAGCGGCCGCGTTTCGGCCTCAGAATCCATTGAAGA
CTTGAACAAGTGGGCCCTATTTCTTGTGTCTCCTTTTATACTTGAAGCAGAACACATAGCATTTGTGACGGAGAGCATTTGGGTACAAAG
TGAGAATTTACAGAGATCATCCTCTTCAGAAACAGTTCGGTTTTTGCCCACTAGGGATGATGTGGTTTCTCATGAGGTTACTTGCTCTAA
AGGACTTTATATTTTGGAACCATAAGAGCACCCTTGTGGCCCAGGCACTTTATGGATGATCCCTTTTAGTGCTCCCAGTAACCTTCCAAG
ATTGTCAAGTGGTCAGACTGTTGTTTGCCATTAGCTTGCAGACCTGGGGATCCTTATCGGCTAATTGCTGAAGCAAGTGTGGACAACTTC
AGCAAGCTGGGGGTGGCGTTCATGGAAGATAGACTCCACATGGATAATGGACTGGTACCCCAAAAGATTGTGTCGGTGCACTTGCAGGAC
TCCACTCTGAAGGAAGTTAAGGATCAGGTCTCAAACAAGCAAGCCCAGATCCTAGAGCCGAAGCCTGAACCTTCTCTTGAGATTAAGCCT
GAGCAGGACGGTATGGAGCATGTTGGCAGAGATGACCCAAAGGCTCTTGGTGAAGAACCCAAACAAAGGAGAGGCAGTGCCTCTGGGAGT
GAGCCTGCTGGGGACAGTGACAGGGGAGGGGGCCCCGTTGAGCATTATCACCTCCATCTGTCTAGTTGCCACGAGTGTCTGGAACTTGAG
AACAGCACCATTGAGTCAGTCAAGTTTGCGTCTGCCGAGAACATTCCAGACCTTCCCTACGATTATAGCAGCAGTTTGGAGAGTGTTGCT
GATGAGACCTCCCCCGAAAGAGAAGGGAGGAGAGTCAACCTCACGGGAAAGGCACCCAACATCCTCCTCTATGTGGGCTCCGACTCCCAG
GAAGCCCTCGGCCGGTTCCACGAGGTCCGGTCTGTGCTGGCCGACTGTGTGGACATTGACAGTTATATTCTCTACCACCTGCTGGAGGAC
AGTGCTCTCAGAGACCCGTGGACGGACAACTGTCTGCTGTTGGTCATTGCTACCAGGGAGTCCATTCCCGAAGACCTGTACCAGAAGTTC
ATGGCCTATCTTTCTCAGGGAGGGAAGGTGTTGGGCCTGTCTTCATCCTTCACCTTTGGTGGCTTTCAGGTGACAAGCAAGGGTGCACTG
CACAAGACAGTCCAGAACTTGGTTTTCTCCAAGGCTGACCAGAGCGAGGTGAAGCTCAGCGTCTTGAGCAGTGGCTGCAGGTACCAGGAA
GGCCCCGTCCGGCTCAGCCCCGGCAGGCTCCAGGGCCACCTGGAGAATGAGGACAAGGACAGGATGATTGTGCATGTGCCTTTTGGAACT
CGCGGGGGAGAAGCTGTTCTTTGCCAGGTGCACTTAGAACTACCTCCCAGCTCCAACATAGTGCAAACTCCAGAAGATTTTAACTTGCTC
AAGTCAAGCAATTTTAGAAGATACGAAGTCCTTAGAGAGATTCTGACAACCCTTGGCCTCAGCTGTGACATGAAACAAGTTCCTGCCTTA
ACTCCTCTTTACTTGCTGTCAGCTGCGGAGGAAATCAGGGATCCTCTTATGCAGTGGCTTGGGAAACATGTGGACTCCGAGGGAGAAATA
AAATCCGGCCAGCTCTCTCTTAGATTTGTTTCATCCTACGTGTCTGAAGTAGAAATAACCCCATCTTGTATACCTGTGGTGACCAACATG
GAGGCCTTCTCATCAGAACATTTCAACTTAGAGATCTATCGCCAAAATCTGCAGACCAAGCAGTTGGGGAAAGTAATTTTGTTTGCCGAA
GTGACCCCCACAACGATGCGTCTCCTGGATGGGCCCGGTGGGATGGCTTGACTGGGCATATCACCTTTAATAAAACCAATGGCTTGAGGA
AGGATTTTGATCTGGACATTATTAGTCTCAAAGAGGAAGGAACTGAAAAGGCTGCTGGCGAAGTGTCTAAACACTTGTATAAAGTGTGGA
AGAAGATTGGGATTTGGAATTCCAACAGTGGGCTTAACATGACGGACAGCAACAAAGACAAGTCCAGCAATATCACTGATTCATTGGCCA
ACAGAACACTCATTGTCACCACCATTCTGGAAGAACCCTATGTTATGTACAGGAAATCTGATAAGCCTCTATATGGAAATGACAGATTTG
AAGGATATTGCCTAGACCTGTTGAAAGAATTGTCAAACATCCTGGGTTTCATTTATGATGTTAAACTAGTTCCCGATGGCAAATATGGGG
CCCAGAATGACAAAGGGGAGTGGAACGGGATGGTTAAAGAACTCATAGATCACAGGGCTGACCTGGCAGTGGCTCCTCTTACCATCACCT
ACGTGCGGGAGAAAGTCATTGACTTCTCCAAACCCTTCATGACCCTAGGCATCAGCATTCTCTACCGGAAGCCCAATGGTACCAATCCAG
GCGTTTTCTCCTTCCTCAACCCCCTGTCTCCAGATATTTGGATGTATGTGCTCTTAGCCTGCTTGGGAGTCAGCTGTGTACTCTTTGTGA
TTGCAAGGTTTACACCCTACGAGTGGTATAACCCCCACCCATGCAACCCTGACTCAGACGTGGTGGAAAACAATTTTACTTTACTAAATA
GTTTCTGGTTTGGAGTTGGAGCTCTCATGCAGCAAGGATCAGAGCTGATGCCCAAAGCTCTATCGACCAGAATAGTTGGAGGGATATGGT
GGTTTTTCACCCTAATCATCATTTCATCCTACACGGCCAATCTGGCTGCCTTCTTGACAGTAGAGAGAATGGAATCCCCCATAGATTCGG
CAGATGATCTGGCAAAGCAAACCAAGATAGAATATGGGGCGGTTAGAGATGGATCAACAATGACCTTCTTCAAGAAATCAAAAATCTCCA
CCTATGAGAAGATGTGGGCTTTCATGAGCAGCAGGCAGCAGACCGCCCTGGTAAGAAACAGTGATGAGGGGATCCAGAGAGTGCTCACCA
CAGACTACGCGCTGCTGATGGAGTCCACCAGCATTGAGTATGTGACGCAGAGAAACTGCAACCTCACTCAGATCGGGGGCCTCATTGACT
CCAAAGGTTACGGAGTGGGAACACCTATTGGTTCTCCTTACCGGGATAAAATTACTATTGCTATTCTTCAACTCCAAGAAGAAGGGAAGC
TGCATATGATGAAAGAGAAGTGGTGGCGTGGGAATGGCTGCCCCGAGGAAGACAACAAAGAAGCCAGTGCCCTGGGAGTGGAAAATATTG
GAGGCATCTTCATTGTTCTGGCTGCCGGACTGGTCCTTTCTGTATTTGTAGCTATTGGAGAATTCATATACAAATCACGGAAGAATAATG
ATATTGAACAGTGTCTCTCTTTCAACGCTATCATGGAAGAACTGGGAATCTCACTGAAGAATCAGAAAAAAATAAAGAAAAAGTCAAGAA

>In-frame_ENST00000336648_ENST00000535441_TCGA-50-5944-01A_HLCS_chr21_38269160_-_GRIK1_chr21_30971257_length(amino acids)=604AA_start in transcript=1775_stop in transcript=3586
MYTCGDQHGGLLIRTFQLRDLSPKSADQAVGESNFVCRSDPHNDASPGWARWDGLTGHITFNKTNGLRKDFDLDIISLKEEGTEKAAGEV
SKHLYKVWKKIGIWNSNSGLNMTDSNKDKSSNITDSLANRTLIVTTILEEPYVMYRKSDKPLYGNDRFEGYCLDLLKELSNILGFIYDVK
LVPDGKYGAQNDKGEWNGMVKELIDHRADLAVAPLTITYVREKVIDFSKPFMTLGISILYRKPNGTNPGVFSFLNPLSPDIWMYVLLACL
GVSCVLFVIARFTPYEWYNPHPCNPDSDVVENNFTLLNSFWFGVGALMQQGSELMPKALSTRIVGGIWWFFTLIIISSYTANLAAFLTVE
RMESPIDSADDLAKQTKIEYGAVRDGSTMTFFKKSKISTYEKMWAFMSSRQQTALVRNSDEGIQRVLTTDYALLMESTSIEYVTQRNCNL
TQIGGLIDSKGYGVGTPIGSPYRDKITIAILQLQEEGKLHMMKEKWWRGNGCPEEDNKEASALGVENIGGIFIVLAAGLVLSVFVAIGEF

--------------------------------------------------------------
>In-frame_ENST00000336648_ENST00000389124_TCGA-50-5944-01A_HLCS_chr21_38269160_-_GRIK1_chr21_30971257_length(transcript)=3504nt_BP=1922nt
ACGAGAGGGCGCAGCGCGGCGCGGCAGGGATTCGCGGGCGACCACCCGGCGCAGGAGCGGCCGCGTTTCGGCCTCAGAATCCATTGAAGA
CTTGAACAAGTGGGCCCTATTTCTTGTGTCTCCTTTTATACTTGAAGCAGAACACATAGCATTTGTGACGGAGAGCATTTGGGTACAAAG
TGAGAATTTACAGAGATCATCCTCTTCAGAAACAGTTCGGTTTTTGCCCACTAGGGATGATGTGGTTTCTCATGAGGTTACTTGCTCTAA
AGGACTTTATATTTTGGAACCATAAGAGCACCCTTGTGGCCCAGGCACTTTATGGATGATCCCTTTTAGTGCTCCCAGTAACCTTCCAAG
ATTGTCAAGTGGTCAGACTGTTGTTTGCCATTAGCTTGCAGACCTGGGGATCCTTATCGGCTAATTGCTGAAGCAAGTGTGGACAACTTC
AGCAAGCTGGGGGTGGCGTTCATGGAAGATAGACTCCACATGGATAATGGACTGGTACCCCAAAAGATTGTGTCGGTGCACTTGCAGGAC
TCCACTCTGAAGGAAGTTAAGGATCAGGTCTCAAACAAGCAAGCCCAGATCCTAGAGCCGAAGCCTGAACCTTCTCTTGAGATTAAGCCT
GAGCAGGACGGTATGGAGCATGTTGGCAGAGATGACCCAAAGGCTCTTGGTGAAGAACCCAAACAAAGGAGAGGCAGTGCCTCTGGGAGT
GAGCCTGCTGGGGACAGTGACAGGGGAGGGGGCCCCGTTGAGCATTATCACCTCCATCTGTCTAGTTGCCACGAGTGTCTGGAACTTGAG
AACAGCACCATTGAGTCAGTCAAGTTTGCGTCTGCCGAGAACATTCCAGACCTTCCCTACGATTATAGCAGCAGTTTGGAGAGTGTTGCT
GATGAGACCTCCCCCGAAAGAGAAGGGAGGAGAGTCAACCTCACGGGAAAGGCACCCAACATCCTCCTCTATGTGGGCTCCGACTCCCAG
GAAGCCCTCGGCCGGTTCCACGAGGTCCGGTCTGTGCTGGCCGACTGTGTGGACATTGACAGTTATATTCTCTACCACCTGCTGGAGGAC
AGTGCTCTCAGAGACCCGTGGACGGACAACTGTCTGCTGTTGGTCATTGCTACCAGGGAGTCCATTCCCGAAGACCTGTACCAGAAGTTC
ATGGCCTATCTTTCTCAGGGAGGGAAGGTGTTGGGCCTGTCTTCATCCTTCACCTTTGGTGGCTTTCAGGTGACAAGCAAGGGTGCACTG
CACAAGACAGTCCAGAACTTGGTTTTCTCCAAGGCTGACCAGAGCGAGGTGAAGCTCAGCGTCTTGAGCAGTGGCTGCAGGTACCAGGAA
GGCCCCGTCCGGCTCAGCCCCGGCAGGCTCCAGGGCCACCTGGAGAATGAGGACAAGGACAGGATGATTGTGCATGTGCCTTTTGGAACT
CGCGGGGGAGAAGCTGTTCTTTGCCAGGTGCACTTAGAACTACCTCCCAGCTCCAACATAGTGCAAACTCCAGAAGATTTTAACTTGCTC
AAGTCAAGCAATTTTAGAAGATACGAAGTCCTTAGAGAGATTCTGACAACCCTTGGCCTCAGCTGTGACATGAAACAAGTTCCTGCCTTA
ACTCCTCTTTACTTGCTGTCAGCTGCGGAGGAAATCAGGGATCCTCTTATGCAGTGGCTTGGGAAACATGTGGACTCCGAGGGAGAAATA
AAATCCGGCCAGCTCTCTCTTAGATTTGTTTCATCCTACGTGTCTGAAGTAGAAATAACCCCATCTTGTATACCTGTGGTGACCAACATG
GAGGCCTTCTCATCAGAACATTTCAACTTAGAGATCTATCGCCAAAATCTGCAGACCAAGCAGTTGGGGAAAGTAATTTTGTTTGCCGAA
GTGACCCCCACAACGATGCGTCTCCTGGATGGGCCCGGTGGGATGGCTTGACTGGGCATATCACCTTTAATAAAACCAATGGCTTGAGGA
AGGATTTTGATCTGGACATTATTAGTCTCAAAGAGGAAGGAACTGAAAAGGCTGCTGGCGAAGTGTCTAAACACTTGTATAAAGTGTGGA
AGAAGATTGGGATTTGGAATTCCAACAGTGGGCTTAACATGACGGACAGCAACAAAGACAAGTCCAGCAATATCACTGATTCATTGGCCA
ACAGAACACTCATTGTCACCACCATTCTGGAAGAACCCTATGTTATGTACAGGAAATCTGATAAGCCTCTATATGGAAATGACAGATTTG
AAGGATATTGCCTAGACCTGTTGAAAGAATTGTCAAACATCCTGGGTTTCATTTATGATGTTAAACTAGTTCCCGATGGCAAATATGGGG
CCCAGAATGACAAAGGGGAGTGGAACGGGATGGTTAAAGAACTCATAGATCACAGGGCTGACCTGGCAGTGGCTCCTCTTACCATCACCT
ACGTGCGGGAGAAAGTCATTGACTTCTCCAAACCCTTCATGACCCTAGGCATCAGCATTCTCTACCGGAAGCCCAATGGTACCAATCCAG
GCGTTTTCTCCTTCCTCAACCCCCTGTCTCCAGATATTTGGATGTATGTGCTCTTAGCCTGCTTGGGAGTCAGCTGTGTACTCTTTGTGA
TTGCAAGGTTTACACCCTACGAGTGGTATAACCCCCACCCATGCAACCCTGACTCAGACGTGGTGGAAAACAATTTTACTTTACTAAATA
GTTTCTGGTTTGGAGTTGGAGCTCTCATGCAGCAAGGATCAGAGCTGATGCCCAAAGCTCTATCGACCAGAATAGTTGGAGGGATATGGT
GGTTTTTCACCCTAATCATCATTTCATCCTACACGGCCAATCTGGCTGCCTTCTTGACAGTAGAGAGAATGGAATCCCCCATAGATTCGG
CAGATGATCTGGCAAAGCAAACCAAGATAGAATATGGGGCGGTTAGAGATGGATCAACAATGACCTTCTTCAAGAAATCAAAAATCTCCA
CCTATGAGAAGATGTGGGCTTTCATGAGCAGCAGGCAGCAGACCGCCCTGGTAAGAAACAGTGATGAGGGGATCCAGAGAGTGCTCACCA
CAGACTACGCGCTGCTGATGGAGTCCACCAGCATTGAGTATGTGACGCAGAGAAACTGCAACCTCACTCAGATCGGGGGCCTCATTGACT
CCAAAGGTTACGGAGTGGGAACACCTATTGGTTCTCCTTACCGGGATAAAATTACTATTGCTATTCTTCAACTCCAAGAAGAAGGGAAGC
TGCATATGATGAAAGAGAAGTGGTGGCGTGGGAATGGCTGCCCCGAGGAAGACAACAAAGAAGCCAGTGCCCTGGGAGTGGAAAATATTG
GAGGCATCTTCATTGTTCTGGCTGCCGGACTGGTCCTTTCTGTATTTGTAGCTATTGGAGAATTCATATACAAATCACGGAAGAATAATG

>In-frame_ENST00000336648_ENST00000389124_TCGA-50-5944-01A_HLCS_chr21_38269160_-_GRIK1_chr21_30971257_length(amino acids)=554AA_start in transcript=1775_stop in transcript=3439
MYTCGDQHGGLLIRTFQLRDLSPKSADQAVGESNFVCRSDPHNDASPGWARWDGLTGHITFNKTNGLRKDFDLDIISLKEEGTEKAAGEV
SKHLYKVWKKIGIWNSNSGLNMTDSNKDKSSNITDSLANRTLIVTTILEEPYVMYRKSDKPLYGNDRFEGYCLDLLKELSNILGFIYDVK
LVPDGKYGAQNDKGEWNGMVKELIDHRADLAVAPLTITYVREKVIDFSKPFMTLGISILYRKPNGTNPGVFSFLNPLSPDIWMYVLLACL
GVSCVLFVIARFTPYEWYNPHPCNPDSDVVENNFTLLNSFWFGVGALMQQGSELMPKALSTRIVGGIWWFFTLIIISSYTANLAAFLTVE
RMESPIDSADDLAKQTKIEYGAVRDGSTMTFFKKSKISTYEKMWAFMSSRQQTALVRNSDEGIQRVLTTDYALLMESTSIEYVTQRNCNL
TQIGGLIDSKGYGVGTPIGSPYRDKITIAILQLQEEGKLHMMKEKWWRGNGCPEEDNKEASALGVENIGGIFIVLAAGLVLSVFVAIGEF

--------------------------------------------------------------
>In-frame_ENST00000336648_ENST00000399907_TCGA-50-5944-01A_HLCS_chr21_38269160_-_GRIK1_chr21_30971257_length(transcript)=3884nt_BP=1922nt
ACGAGAGGGCGCAGCGCGGCGCGGCAGGGATTCGCGGGCGACCACCCGGCGCAGGAGCGGCCGCGTTTCGGCCTCAGAATCCATTGAAGA
CTTGAACAAGTGGGCCCTATTTCTTGTGTCTCCTTTTATACTTGAAGCAGAACACATAGCATTTGTGACGGAGAGCATTTGGGTACAAAG
TGAGAATTTACAGAGATCATCCTCTTCAGAAACAGTTCGGTTTTTGCCCACTAGGGATGATGTGGTTTCTCATGAGGTTACTTGCTCTAA
AGGACTTTATATTTTGGAACCATAAGAGCACCCTTGTGGCCCAGGCACTTTATGGATGATCCCTTTTAGTGCTCCCAGTAACCTTCCAAG
ATTGTCAAGTGGTCAGACTGTTGTTTGCCATTAGCTTGCAGACCTGGGGATCCTTATCGGCTAATTGCTGAAGCAAGTGTGGACAACTTC
AGCAAGCTGGGGGTGGCGTTCATGGAAGATAGACTCCACATGGATAATGGACTGGTACCCCAAAAGATTGTGTCGGTGCACTTGCAGGAC
TCCACTCTGAAGGAAGTTAAGGATCAGGTCTCAAACAAGCAAGCCCAGATCCTAGAGCCGAAGCCTGAACCTTCTCTTGAGATTAAGCCT
GAGCAGGACGGTATGGAGCATGTTGGCAGAGATGACCCAAAGGCTCTTGGTGAAGAACCCAAACAAAGGAGAGGCAGTGCCTCTGGGAGT
GAGCCTGCTGGGGACAGTGACAGGGGAGGGGGCCCCGTTGAGCATTATCACCTCCATCTGTCTAGTTGCCACGAGTGTCTGGAACTTGAG
AACAGCACCATTGAGTCAGTCAAGTTTGCGTCTGCCGAGAACATTCCAGACCTTCCCTACGATTATAGCAGCAGTTTGGAGAGTGTTGCT
GATGAGACCTCCCCCGAAAGAGAAGGGAGGAGAGTCAACCTCACGGGAAAGGCACCCAACATCCTCCTCTATGTGGGCTCCGACTCCCAG
GAAGCCCTCGGCCGGTTCCACGAGGTCCGGTCTGTGCTGGCCGACTGTGTGGACATTGACAGTTATATTCTCTACCACCTGCTGGAGGAC
AGTGCTCTCAGAGACCCGTGGACGGACAACTGTCTGCTGTTGGTCATTGCTACCAGGGAGTCCATTCCCGAAGACCTGTACCAGAAGTTC
ATGGCCTATCTTTCTCAGGGAGGGAAGGTGTTGGGCCTGTCTTCATCCTTCACCTTTGGTGGCTTTCAGGTGACAAGCAAGGGTGCACTG
CACAAGACAGTCCAGAACTTGGTTTTCTCCAAGGCTGACCAGAGCGAGGTGAAGCTCAGCGTCTTGAGCAGTGGCTGCAGGTACCAGGAA
GGCCCCGTCCGGCTCAGCCCCGGCAGGCTCCAGGGCCACCTGGAGAATGAGGACAAGGACAGGATGATTGTGCATGTGCCTTTTGGAACT
CGCGGGGGAGAAGCTGTTCTTTGCCAGGTGCACTTAGAACTACCTCCCAGCTCCAACATAGTGCAAACTCCAGAAGATTTTAACTTGCTC
AAGTCAAGCAATTTTAGAAGATACGAAGTCCTTAGAGAGATTCTGACAACCCTTGGCCTCAGCTGTGACATGAAACAAGTTCCTGCCTTA
ACTCCTCTTTACTTGCTGTCAGCTGCGGAGGAAATCAGGGATCCTCTTATGCAGTGGCTTGGGAAACATGTGGACTCCGAGGGAGAAATA
AAATCCGGCCAGCTCTCTCTTAGATTTGTTTCATCCTACGTGTCTGAAGTAGAAATAACCCCATCTTGTATACCTGTGGTGACCAACATG
GAGGCCTTCTCATCAGAACATTTCAACTTAGAGATCTATCGCCAAAATCTGCAGACCAAGCAGTTGGGGAAAGTAATTTTGTTTGCCGAA
GTGACCCCCACAACGATGCGTCTCCTGGATGGGCCCGGTGGGATGGCTTGACTGGGCATATCACCTTTAATAAAACCAATGGCTTGAGGA
AGGATTTTGATCTGGACATTATTAGTCTCAAAGAGGAAGGAACTGAAAAGGCTGCTGGCGAAGTGTCTAAACACTTGTATAAAGTGTGGA
AGAAGATTGGGATTTGGAATTCCAACAGTGGGCTTAACATGACGGACAGCAACAAAGACAAGTCCAGCAATATCACTGATTCATTGGCCA
ACAGAACACTCATTGTCACCACCATTCTGGAAGAACCCTATGTTATGTACAGGAAATCTGATAAGCCTCTATATGGAAATGACAGATTTG
AAGGATATTGCCTAGACCTGTTGAAAGAATTGTCAAACATCCTGGGTTTCATTTATGATGTTAAACTAGTTCCCGATGGCAAATATGGGG
CCCAGAATGACAAAGGGGAGTGGAACGGGATGGTTAAAGAACTCATAGATCACAGGGCTGACCTGGCAGTGGCTCCTCTTACCATCACCT
ACGTGCGGGAGAAAGTCATTGACTTCTCCAAACCCTTCATGACCCTAGGCATCAGCATTCTCTACCGGAAGCCCAATGGTACCAATCCAG
GCGTTTTCTCCTTCCTCAACCCCCTGTCTCCAGATATTTGGATGTATGTGCTCTTAGCCTGCTTGGGAGTCAGCTGTGTACTCTTTGTGA
TTGCAAGGTTTACACCCTACGAGTGGTATAACCCCCACCCATGCAACCCTGACTCAGACGTGGTGGAAAACAATTTTACTTTACTAAATA
GTTTCTGGTTTGGAGTTGGAGCTCTCATGCAGCAAGGATCAGAGCTGATGCCCAAAGCTCTATCGACCAGAATAGTTGGAGGGATATGGT
GGTTTTTCACCCTAATCATCATTTCATCCTACACGGCCAATCTGGCTGCCTTCTTGACAGTAGAGAGAATGGAATCCCCCATAGATTCGG
CAGATGATCTGGCAAAGCAAACCAAGATAGAATATGGGGCGGTTAGAGATGGATCAACAATGACCTTCTTCAAGAAATCAAAAATCTCCA
CCTATGAGAAGATGTGGGCTTTCATGAGCAGCAGGCAGCAGACCGCCCTGGTAAGAAACAGTGATGAGGGGATCCAGAGAGTGCTCACCA
CAGACTACGCGCTGCTGATGGAGTCCACCAGCATTGAGTATGTGACGCAGAGAAACTGCAACCTCACTCAGATCGGGGGCCTCATTGACT
CCAAAGGTTACGGAGTGGGAACACCTATTGGTTCTCCTTACCGGGATAAAATTACTATTGCTATTCTTCAACTCCAAGAAGAAGGGAAGC
TGCATATGATGAAAGAGAAGTGGTGGCGTGGGAATGGCTGCCCCGAGGAAGACAACAAAGAAGCCAGTGCCCTGGGAGTGGAAAATATTG
GAGGCATCTTCATTGTTCTGGCTGCCGGACTGGTCCTTTCTGTATTTGTAGCTATTGGAGAATTCATATACAAATCACGGAAGAATAATG
ATATTGAACAGGCTTTTTGTTTCTTTTATGGACTGCAATGTAAGCAAACCCATCCAACCAACTCCACTTCTGGAACTACTTTATCTACGG
ATTTAGAATGTGGTAAATTAATTCGAGAGGAGAGAGGGATTCGAAAACAGTCCTCAGTTCATACTGTGTAATCAGTTTAAAAAAAAAAAA
AAAGATGTGCCCAGTAGAAAATGTTTTTGCTAATTGGTGTTGTTGATATTTGTATTTGCTAAGCATAAATAGCCACAATTCTCTATTCCT
TTAGGATAGCAAAACTCATGTATTCTAGACATTGAAATGAATACACTGGAAAGATACTTGCACTGTATGTTGAAGCAGGGGCATATTAGG
GGTATAAAGACTTCTAGAAATGTGTATTTACAGGAATCTTCTCTGTCCCACTCATCATCTTAGTTCTATCTTCATGCATTAAAAATAAGT

>In-frame_ENST00000336648_ENST00000399907_TCGA-50-5944-01A_HLCS_chr21_38269160_-_GRIK1_chr21_30971257_length(amino acids)=601AA_start in transcript=1775_stop in transcript=3580
MYTCGDQHGGLLIRTFQLRDLSPKSADQAVGESNFVCRSDPHNDASPGWARWDGLTGHITFNKTNGLRKDFDLDIISLKEEGTEKAAGEV
SKHLYKVWKKIGIWNSNSGLNMTDSNKDKSSNITDSLANRTLIVTTILEEPYVMYRKSDKPLYGNDRFEGYCLDLLKELSNILGFIYDVK
LVPDGKYGAQNDKGEWNGMVKELIDHRADLAVAPLTITYVREKVIDFSKPFMTLGISILYRKPNGTNPGVFSFLNPLSPDIWMYVLLACL
GVSCVLFVIARFTPYEWYNPHPCNPDSDVVENNFTLLNSFWFGVGALMQQGSELMPKALSTRIVGGIWWFFTLIIISSYTANLAAFLTVE
RMESPIDSADDLAKQTKIEYGAVRDGSTMTFFKKSKISTYEKMWAFMSSRQQTALVRNSDEGIQRVLTTDYALLMESTSIEYVTQRNCNL
TQIGGLIDSKGYGVGTPIGSPYRDKITIAILQLQEEGKLHMMKEKWWRGNGCPEEDNKEASALGVENIGGIFIVLAAGLVLSVFVAIGEF

--------------------------------------------------------------
>In-frame_ENST00000336648_ENST00000399909_TCGA-50-5944-01A_HLCS_chr21_38269160_-_GRIK1_chr21_30971257_length(transcript)=3839nt_BP=1922nt
ACGAGAGGGCGCAGCGCGGCGCGGCAGGGATTCGCGGGCGACCACCCGGCGCAGGAGCGGCCGCGTTTCGGCCTCAGAATCCATTGAAGA
CTTGAACAAGTGGGCCCTATTTCTTGTGTCTCCTTTTATACTTGAAGCAGAACACATAGCATTTGTGACGGAGAGCATTTGGGTACAAAG
TGAGAATTTACAGAGATCATCCTCTTCAGAAACAGTTCGGTTTTTGCCCACTAGGGATGATGTGGTTTCTCATGAGGTTACTTGCTCTAA
AGGACTTTATATTTTGGAACCATAAGAGCACCCTTGTGGCCCAGGCACTTTATGGATGATCCCTTTTAGTGCTCCCAGTAACCTTCCAAG
ATTGTCAAGTGGTCAGACTGTTGTTTGCCATTAGCTTGCAGACCTGGGGATCCTTATCGGCTAATTGCTGAAGCAAGTGTGGACAACTTC
AGCAAGCTGGGGGTGGCGTTCATGGAAGATAGACTCCACATGGATAATGGACTGGTACCCCAAAAGATTGTGTCGGTGCACTTGCAGGAC
TCCACTCTGAAGGAAGTTAAGGATCAGGTCTCAAACAAGCAAGCCCAGATCCTAGAGCCGAAGCCTGAACCTTCTCTTGAGATTAAGCCT
GAGCAGGACGGTATGGAGCATGTTGGCAGAGATGACCCAAAGGCTCTTGGTGAAGAACCCAAACAAAGGAGAGGCAGTGCCTCTGGGAGT
GAGCCTGCTGGGGACAGTGACAGGGGAGGGGGCCCCGTTGAGCATTATCACCTCCATCTGTCTAGTTGCCACGAGTGTCTGGAACTTGAG
AACAGCACCATTGAGTCAGTCAAGTTTGCGTCTGCCGAGAACATTCCAGACCTTCCCTACGATTATAGCAGCAGTTTGGAGAGTGTTGCT
GATGAGACCTCCCCCGAAAGAGAAGGGAGGAGAGTCAACCTCACGGGAAAGGCACCCAACATCCTCCTCTATGTGGGCTCCGACTCCCAG
GAAGCCCTCGGCCGGTTCCACGAGGTCCGGTCTGTGCTGGCCGACTGTGTGGACATTGACAGTTATATTCTCTACCACCTGCTGGAGGAC
AGTGCTCTCAGAGACCCGTGGACGGACAACTGTCTGCTGTTGGTCATTGCTACCAGGGAGTCCATTCCCGAAGACCTGTACCAGAAGTTC
ATGGCCTATCTTTCTCAGGGAGGGAAGGTGTTGGGCCTGTCTTCATCCTTCACCTTTGGTGGCTTTCAGGTGACAAGCAAGGGTGCACTG
CACAAGACAGTCCAGAACTTGGTTTTCTCCAAGGCTGACCAGAGCGAGGTGAAGCTCAGCGTCTTGAGCAGTGGCTGCAGGTACCAGGAA
GGCCCCGTCCGGCTCAGCCCCGGCAGGCTCCAGGGCCACCTGGAGAATGAGGACAAGGACAGGATGATTGTGCATGTGCCTTTTGGAACT
CGCGGGGGAGAAGCTGTTCTTTGCCAGGTGCACTTAGAACTACCTCCCAGCTCCAACATAGTGCAAACTCCAGAAGATTTTAACTTGCTC
AAGTCAAGCAATTTTAGAAGATACGAAGTCCTTAGAGAGATTCTGACAACCCTTGGCCTCAGCTGTGACATGAAACAAGTTCCTGCCTTA
ACTCCTCTTTACTTGCTGTCAGCTGCGGAGGAAATCAGGGATCCTCTTATGCAGTGGCTTGGGAAACATGTGGACTCCGAGGGAGAAATA
AAATCCGGCCAGCTCTCTCTTAGATTTGTTTCATCCTACGTGTCTGAAGTAGAAATAACCCCATCTTGTATACCTGTGGTGACCAACATG
GAGGCCTTCTCATCAGAACATTTCAACTTAGAGATCTATCGCCAAAATCTGCAGACCAAGCAGTTGGGGAAAGTAATTTTGTTTGCCGAA
GTGACCCCCACAACGATGCGTCTCCTGGATGGGCCCGGTGGGATGGCTTGACTGGGCATATCACCTTTAATAAAACCAATGGCTTGAGGA
AGGATTTTGATCTGGACATTATTAGTCTCAAAGAGGAAGGAACTGAAAAGATTGGGATTTGGAATTCCAACAGTGGGCTTAACATGACGG
ACAGCAACAAAGACAAGTCCAGCAATATCACTGATTCATTGGCCAACAGAACACTCATTGTCACCACCATTCTGGAAGAACCCTATGTTA
TGTACAGGAAATCTGATAAGCCTCTATATGGAAATGACAGATTTGAAGGATATTGCCTAGACCTGTTGAAAGAATTGTCAAACATCCTGG
GTTTCATTTATGATGTTAAACTAGTTCCCGATGGCAAATATGGGGCCCAGAATGACAAAGGGGAGTGGAACGGGATGGTTAAAGAACTCA
TAGATCACAGGGCTGACCTGGCAGTGGCTCCTCTTACCATCACCTACGTGCGGGAGAAAGTCATTGACTTCTCCAAACCCTTCATGACCC
TAGGCATCAGCATTCTCTACCGGAAGCCCAATGGTACCAATCCAGGCGTTTTCTCCTTCCTCAACCCCCTGTCTCCAGATATTTGGATGT
ATGTGCTCTTAGCCTGCTTGGGAGTCAGCTGTGTACTCTTTGTGATTGCAAGGTTTACACCCTACGAGTGGTATAACCCCCACCCATGCA
ACCCTGACTCAGACGTGGTGGAAAACAATTTTACTTTACTAAATAGTTTCTGGTTTGGAGTTGGAGCTCTCATGCAGCAAGGATCAGAGC
TGATGCCCAAAGCTCTATCGACCAGAATAGTTGGAGGGATATGGTGGTTTTTCACCCTAATCATCATTTCATCCTACACGGCCAATCTGG
CTGCCTTCTTGACAGTAGAGAGAATGGAATCCCCCATAGATTCGGCAGATGATCTGGCAAAGCAAACCAAGATAGAATATGGGGCGGTTA
GAGATGGATCAACAATGACCTTCTTCAAGAAATCAAAAATCTCCACCTATGAGAAGATGTGGGCTTTCATGAGCAGCAGGCAGCAGACCG
CCCTGGTAAGAAACAGTGATGAGGGGATCCAGAGAGTGCTCACCACAGACTACGCGCTGCTGATGGAGTCCACCAGCATTGAGTATGTGA
CGCAGAGAAACTGCAACCTCACTCAGATCGGGGGCCTCATTGACTCCAAAGGTTACGGAGTGGGAACACCTATTGGTTCTCCTTACCGGG
ATAAAATTACTATTGCTATTCTTCAACTCCAAGAAGAAGGGAAGCTGCATATGATGAAAGAGAAGTGGTGGCGTGGGAATGGCTGCCCCG
AGGAAGACAACAAAGAAGCCAGTGCCCTGGGAGTGGAAAATATTGGAGGCATCTTCATTGTTCTGGCTGCCGGACTGGTCCTTTCTGTAT
TTGTAGCTATTGGAGAATTCATATACAAATCACGGAAGAATAATGATATTGAACAGGCTTTTTGTTTCTTTTATGGACTGCAATGTAAGC
AAACCCATCCAACCAACTCCACTTCTGGAACTACTTTATCTACGGATTTAGAATGTGGTAAATTAATTCGAGAGGAGAGAGGGATTCGAA
AACAGTCCTCAGTTCATACTGTGTAATCAGTTTAAAAAAAAAAAAAAAGATGTGCCCAGTAGAAAATGTTTTTGCTAATTGGTGTTGTTG
ATATTTGTATTTGCTAAGCATAAATAGCCACAATTCTCTATTCCTTTAGGATAGCAAAACTCATGTATTCTAGACATTGAAATGAATACA
CTGGAAAGATACTTGCACTGTATGTTGAAGCAGGGGCATATTAGGGGTATAAAGACTTCTAGAAATGTGTATTTACAGGAATCTTCTCTG

>In-frame_ENST00000336648_ENST00000399909_TCGA-50-5944-01A_HLCS_chr21_38269160_-_GRIK1_chr21_30971257_length(amino acids)=586AA_start in transcript=1775_stop in transcript=3535
MYTCGDQHGGLLIRTFQLRDLSPKSADQAVGESNFVCRSDPHNDASPGWARWDGLTGHITFNKTNGLRKDFDLDIISLKEEGTEKIGIWN
SNSGLNMTDSNKDKSSNITDSLANRTLIVTTILEEPYVMYRKSDKPLYGNDRFEGYCLDLLKELSNILGFIYDVKLVPDGKYGAQNDKGE
WNGMVKELIDHRADLAVAPLTITYVREKVIDFSKPFMTLGISILYRKPNGTNPGVFSFLNPLSPDIWMYVLLACLGVSCVLFVIARFTPY
EWYNPHPCNPDSDVVENNFTLLNSFWFGVGALMQQGSELMPKALSTRIVGGIWWFFTLIIISSYTANLAAFLTVERMESPIDSADDLAKQ
TKIEYGAVRDGSTMTFFKKSKISTYEKMWAFMSSRQQTALVRNSDEGIQRVLTTDYALLMESTSIEYVTQRNCNLTQIGGLIDSKGYGVG
TPIGSPYRDKITIAILQLQEEGKLHMMKEKWWRGNGCPEEDNKEASALGVENIGGIFIVLAAGLVLSVFVAIGEFIYKSRKNNDIEQAFC

--------------------------------------------------------------
>In-frame_ENST00000336648_ENST00000309434_TCGA-50-5944-01A_HLCS_chr21_38269160_-_GRIK1_chr21_30971257_length(transcript)=3581nt_BP=1922nt
ACGAGAGGGCGCAGCGCGGCGCGGCAGGGATTCGCGGGCGACCACCCGGCGCAGGAGCGGCCGCGTTTCGGCCTCAGAATCCATTGAAGA
CTTGAACAAGTGGGCCCTATTTCTTGTGTCTCCTTTTATACTTGAAGCAGAACACATAGCATTTGTGACGGAGAGCATTTGGGTACAAAG
TGAGAATTTACAGAGATCATCCTCTTCAGAAACAGTTCGGTTTTTGCCCACTAGGGATGATGTGGTTTCTCATGAGGTTACTTGCTCTAA
AGGACTTTATATTTTGGAACCATAAGAGCACCCTTGTGGCCCAGGCACTTTATGGATGATCCCTTTTAGTGCTCCCAGTAACCTTCCAAG
ATTGTCAAGTGGTCAGACTGTTGTTTGCCATTAGCTTGCAGACCTGGGGATCCTTATCGGCTAATTGCTGAAGCAAGTGTGGACAACTTC
AGCAAGCTGGGGGTGGCGTTCATGGAAGATAGACTCCACATGGATAATGGACTGGTACCCCAAAAGATTGTGTCGGTGCACTTGCAGGAC
TCCACTCTGAAGGAAGTTAAGGATCAGGTCTCAAACAAGCAAGCCCAGATCCTAGAGCCGAAGCCTGAACCTTCTCTTGAGATTAAGCCT
GAGCAGGACGGTATGGAGCATGTTGGCAGAGATGACCCAAAGGCTCTTGGTGAAGAACCCAAACAAAGGAGAGGCAGTGCCTCTGGGAGT
GAGCCTGCTGGGGACAGTGACAGGGGAGGGGGCCCCGTTGAGCATTATCACCTCCATCTGTCTAGTTGCCACGAGTGTCTGGAACTTGAG
AACAGCACCATTGAGTCAGTCAAGTTTGCGTCTGCCGAGAACATTCCAGACCTTCCCTACGATTATAGCAGCAGTTTGGAGAGTGTTGCT
GATGAGACCTCCCCCGAAAGAGAAGGGAGGAGAGTCAACCTCACGGGAAAGGCACCCAACATCCTCCTCTATGTGGGCTCCGACTCCCAG
GAAGCCCTCGGCCGGTTCCACGAGGTCCGGTCTGTGCTGGCCGACTGTGTGGACATTGACAGTTATATTCTCTACCACCTGCTGGAGGAC
AGTGCTCTCAGAGACCCGTGGACGGACAACTGTCTGCTGTTGGTCATTGCTACCAGGGAGTCCATTCCCGAAGACCTGTACCAGAAGTTC
ATGGCCTATCTTTCTCAGGGAGGGAAGGTGTTGGGCCTGTCTTCATCCTTCACCTTTGGTGGCTTTCAGGTGACAAGCAAGGGTGCACTG
CACAAGACAGTCCAGAACTTGGTTTTCTCCAAGGCTGACCAGAGCGAGGTGAAGCTCAGCGTCTTGAGCAGTGGCTGCAGGTACCAGGAA
GGCCCCGTCCGGCTCAGCCCCGGCAGGCTCCAGGGCCACCTGGAGAATGAGGACAAGGACAGGATGATTGTGCATGTGCCTTTTGGAACT
CGCGGGGGAGAAGCTGTTCTTTGCCAGGTGCACTTAGAACTACCTCCCAGCTCCAACATAGTGCAAACTCCAGAAGATTTTAACTTGCTC
AAGTCAAGCAATTTTAGAAGATACGAAGTCCTTAGAGAGATTCTGACAACCCTTGGCCTCAGCTGTGACATGAAACAAGTTCCTGCCTTA
ACTCCTCTTTACTTGCTGTCAGCTGCGGAGGAAATCAGGGATCCTCTTATGCAGTGGCTTGGGAAACATGTGGACTCCGAGGGAGAAATA
AAATCCGGCCAGCTCTCTCTTAGATTTGTTTCATCCTACGTGTCTGAAGTAGAAATAACCCCATCTTGTATACCTGTGGTGACCAACATG
GAGGCCTTCTCATCAGAACATTTCAACTTAGAGATCTATCGCCAAAATCTGCAGACCAAGCAGTTGGGGAAAGTAATTTTGTTTGCCGAA
GTGACCCCCACAACGATGCGTCTCCTGGATGGGCCCGGTGGGATGGCTTGACTGGGCATATCACCTTTAATAAAACCAATGGCTTGAGGA
AGGATTTTGATCTGGACATTATTAGTCTCAAAGAGGAAGGAACTGAAAAGGCTGCTGGCGAAGTGTCTAAACACTTGTATAAAGTGTGGA
AGAAGATTGGGATTTGGAATTCCAACAGTGGGCTTAACATGACGGACAGCAACAAAGACAAGTCCAGCAATATCACTGATTCATTGGCCA
ACAGAACACTCATTGTCACCACCATTCTGGAAGAACCCTATGTTATGTACAGGAAATCTGATAAGCCTCTATATGGAAATGACAGATTTG
AAGGATATTGCCTAGACCTGTTGAAAGAATTGTCAAACATCCTGGGTTTCATTTATGATGTTAAACTAGTTCCCGATGGCAAATATGGGG
CCCAGAATGACAAAGGGGAGTGGAACGGGATGGTTAAAGAACTCATAGATCACAGGGCTGACCTGGCAGTGGCTCCTCTTACCATCACCT
ACGTGCGGGAGAAAGTCATTGACTTCTCCAAACCCTTCATGACCCTAGGCATCAGCATTCTCTACCGGAAGCCCAATGGTACCAATCCAG
GCGTTTTCTCCTTCCTCAACCCCCTGTCTCCAGATATTTGGATGTATGTGCTCTTAGCCTGCTTGGGAGTCAGCTGTGTACTCTTTGTGA
TTGCAAGGTTTACACCCTACGAGTGGTATAACCCCCACCCATGCAACCCTGACTCAGACGTGGTGGAAAACAATTTTACTTTACTAAATA
GTTTCTGGTTTGGAGTTGGAGCTCTCATGCAGCAAGGATCAGAGCTGATGCCCAAAGCTCTATCGACCAGAATAGTTGGAGGGATATGGT
GGTTTTTCACCCTAATCATCATTTCATCCTACACGGCCAATCTGGCTGCCTTCTTGACAGTAGAGAGAATGGAATCCCCCATAGATTCGG
CAGATGATCTGGCAAAGCAAACCAAGATAGAATATGGGGCGGTTAGAGATGGATCAACAATGACCTTCTTCAAGAAATCAAAAATCTCCA
CCTATGAGAAGATGTGGGCTTTCATGAGCAGCAGGCAGCAGACCGCCCTGGTAAGAAACAGTGATGAGGGGATCCAGAGAGTGCTCACCA
CAGACTACGCGCTGCTGATGGAGTCCACCAGCATTGAGTATGTGACGCAGAGAAACTGCAACCTCACTCAGATCGGGGGCCTCATTGACT
CCAAAGGTTACGGAGTGGGAACACCTATTGGTTCTCCTTACCGGGATAAAATTACTATTGCTATTCTTCAACTCCAAGAAGAAGGGAAGC
TGCATATGATGAAAGAGAAGTGGTGGCGTGGGAATGGCTGCCCCGAGGAAGACAACAAAGAAGCCAGTGCCCTGGGAGTGGAAAATATTG
GAGGCATCTTCATTGTTCTGGCTGCCGGACTGGTCCTTTCTGTATTTGTAGCTATTGGAGAATTCATATACAAATCACGGAAGAATAATG
ATATTGAACAGGCTTTTTGTTTCTTTTATGGACTGCAATGTAAGCAAACCCATCCAACCAACTCCACTTCTGGAACTACTTTATCTACGG

>In-frame_ENST00000336648_ENST00000309434_TCGA-50-5944-01A_HLCS_chr21_38269160_-_GRIK1_chr21_30971257_length(amino acids)=602AA_start in transcript=1775_stop in transcript=3580
MYTCGDQHGGLLIRTFQLRDLSPKSADQAVGESNFVCRSDPHNDASPGWARWDGLTGHITFNKTNGLRKDFDLDIISLKEEGTEKAAGEV
SKHLYKVWKKIGIWNSNSGLNMTDSNKDKSSNITDSLANRTLIVTTILEEPYVMYRKSDKPLYGNDRFEGYCLDLLKELSNILGFIYDVK
LVPDGKYGAQNDKGEWNGMVKELIDHRADLAVAPLTITYVREKVIDFSKPFMTLGISILYRKPNGTNPGVFSFLNPLSPDIWMYVLLACL
GVSCVLFVIARFTPYEWYNPHPCNPDSDVVENNFTLLNSFWFGVGALMQQGSELMPKALSTRIVGGIWWFFTLIIISSYTANLAAFLTVE
RMESPIDSADDLAKQTKIEYGAVRDGSTMTFFKKSKISTYEKMWAFMSSRQQTALVRNSDEGIQRVLTTDYALLMESTSIEYVTQRNCNL
TQIGGLIDSKGYGVGTPIGSPYRDKITIAILQLQEEGKLHMMKEKWWRGNGCPEEDNKEASALGVENIGGIFIVLAAGLVLSVFVAIGEF

--------------------------------------------------------------

Top

Fusion Gene PPI Analysis for HLCS-GRIK1


check button Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in

ChiPPI page.


check button Protein-protein interactors with each fusion partner protein in wild-type (BIOGRID-3.4.160)
HgeneHgene's interactorsTgeneTgene's interactors


check button - Retained PPIs in in-frame fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenStill interaction with


check button - Lost PPIs in in-frame fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenInteraction lost with


check button - Retained PPIs, but lost function due to frame-shift fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenInteraction lost with


Top

Related Drugs for HLCS-GRIK1


check button Drugs targeting genes involved in this fusion gene.
(DrugBank Version 5.1.8 2021-05-08)
PartnerGeneUniProtAccDrugBank IDDrug nameDrug activityDrug typeDrug status
TgeneGRIK1P39086DB00237ButabarbitalAntagonistSmall moleculeApproved|Illicit
TgeneGRIK1P39086DB00237ButabarbitalAntagonistSmall moleculeApproved|Illicit
TgeneGRIK1P39086DB00142Glutamic acidSmall moleculeApproved|Nutraceutical
TgeneGRIK1P39086DB00142Glutamic acidSmall moleculeApproved|Nutraceutical

Top

Related Diseases for HLCS-GRIK1


check button Diseases associated with fusion partners.
(DisGeNet 4.0)
PartnerGeneDisease IDDisease name# pubmedsSource
HgeneHLCSC0268581Holocarboxylase Synthetase Deficiency14CTD_human;GENOMICS_ENGLAND;ORPHANET;UNIPROT
TgeneGRIK1C0036341Schizophrenia2PSYGENET
TgeneGRIK1C0687132heavy drinking2PSYGENET
TgeneGRIK1C0001969Alcoholic Intoxication1PSYGENET
TgeneGRIK1C0001973Alcoholic Intoxication, Chronic1PSYGENET
TgeneGRIK1C0005586Bipolar Disorder1PSYGENET
TgeneGRIK1C0022333Jacksonian Seizure1CTD_human
TgeneGRIK1C0036572Seizures1CTD_human
TgeneGRIK1C0149958Complex partial seizures1CTD_human
TgeneGRIK1C0234533Generalized seizures1CTD_human
TgeneGRIK1C0234535Clonic Seizures1CTD_human
TgeneGRIK1C0270824Visual seizure1CTD_human
TgeneGRIK1C0270844Tonic Seizures1CTD_human
TgeneGRIK1C0270846Epileptic drop attack1CTD_human
TgeneGRIK1C0422850Seizures, Somatosensory1CTD_human
TgeneGRIK1C0422852Seizures, Auditory1CTD_human
TgeneGRIK1C0422853Olfactory seizure1CTD_human
TgeneGRIK1C0422854Gustatory seizure1CTD_human
TgeneGRIK1C0422855Vertiginous seizure1CTD_human
TgeneGRIK1C0494475Tonic - clonic seizures1CTD_human
TgeneGRIK1C0573196Overdose of cocaine1PSYGENET
TgeneGRIK1C0751056Non-epileptic convulsion1CTD_human
TgeneGRIK1C0751110Single Seizure1CTD_human
TgeneGRIK1C0751123Atonic Absence Seizures1CTD_human
TgeneGRIK1C0751494Convulsive Seizures1CTD_human
TgeneGRIK1C0751495Seizures, Focal1CTD_human
TgeneGRIK1C0751496Seizures, Sensory1CTD_human
TgeneGRIK1C3495874Nonepileptic Seizures1CTD_human
TgeneGRIK1C3496069cocaine use1PSYGENET
TgeneGRIK1C4048158Convulsions1CTD_human
TgeneGRIK1C4316903Absence Seizures1CTD_human
TgeneGRIK1C4317109Epileptic Seizures1CTD_human
TgeneGRIK1C4317123Myoclonic Seizures1CTD_human
TgeneGRIK1C4505436Generalized Absence Seizures1CTD_human