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Fusion Gene Summary | |
Fusion Gene ORF analysis | |
Fusion Genomic Features | |
Fusion Protein Features | |
Fusion Gene Sequence | |
Fusion Gene PPI analysis | |
Related Drugs | |
Related Diseases |
Fusion gene:MACF1-BMP8A (FusionGDB2 ID:50333) |
Fusion Gene Summary for MACF1-BMP8A |
Fusion gene summary |
Fusion gene information | Fusion gene name: MACF1-BMP8A | Fusion gene ID: 50333 | Hgene | Tgene | Gene symbol | MACF1 | BMP8A | Gene ID | 23499 | 353500 |
Gene name | microtubule actin crosslinking factor 1 | bone morphogenetic protein 8a | |
Synonyms | ABP620|ACF7|LIS9|MACF|OFC4 | OP-2 | |
Cytomap | 1p34.3 | 1p34.3 | |
Type of gene | protein-coding | protein-coding | |
Description | microtubule-actin cross-linking factor 1620 kDa actin binding proteinactin cross-linking family protein 7macrophin 1 isoformtrabeculin-alpha | bone morphogenetic protein 8ABMP-8A | |
Modification date | 20200313 | 20200313 | |
UniProtAcc | Q9UPN3 | . | |
Ensembl transtripts involved in fusion gene | ENST00000545844, ENST00000567887, ENST00000372915, ENST00000361689, ENST00000539005, ENST00000317713, ENST00000564288, ENST00000289893, ENST00000536367, ENST00000484793, ENST00000602421, ENST00000476350, | ENST00000331593, | |
Fusion gene scores | * DoF score | 46 X 37 X 13=22126 | 3 X 4 X 4=48 |
# samples | 44 | 4 | |
** MAII score | log2(44/22126*10)=-5.65209532630239 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0 | log2(4/48*10)=-0.263034405833794 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0 | |
Context | PubMed: MACF1 [Title/Abstract] AND BMP8A [Title/Abstract] AND fusion [Title/Abstract] | ||
Most frequent breakpoint | MACF1(39854330)-BMP8A(39987047), # samples:1 MACF1(39571389)-BMP8A(39976846), # samples:1 | ||
Anticipated loss of major functional domain due to fusion event. |
* DoF score (Degree of Frequency) = # partners X # break points X # cancer types ** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10) |
Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez |
Partner | Gene | GO ID | GO term | PubMed ID |
Hgene | MACF1 | GO:0030334 | regulation of cell migration | 27693509 |
Hgene | MACF1 | GO:0043001 | Golgi to plasma membrane protein transport | 15265687 |
Hgene | MACF1 | GO:0045773 | positive regulation of axon extension | 22705394 |
Hgene | MACF1 | GO:0051893 | regulation of focal adhesion assembly | 27693509 |
Tgene | BMP8A | GO:0002024 | diet induced thermogenesis | 22579288 |
Tgene | BMP8A | GO:0046676 | negative regulation of insulin secretion | 22579288 |
Tgene | BMP8A | GO:0097009 | energy homeostasis | 22579288 |
Fusion gene breakpoints across MACF1 (5'-gene) * Click on the image to open the UCSC genome browser with custom track showing this image in a new window. |
Fusion gene breakpoints across BMP8A (3'-gene) * Click on the image to open the UCSC genome browser with custom track showing this image in a new window. |
Fusion gene information from two resources (ChiTars 5.0 and ChimerDB 4.0) * All genome coordinats were lifted-over on hg19. * Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser. |
Source | Disease | Sample | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand |
ChimerDB4 | OV | TCGA-24-1469 | MACF1 | chr1 | 39854330 | + | BMP8A | chr1 | 39987047 | + |
ChimerDB4 | SARC | TCGA-X9-A971-01A | MACF1 | chr1 | 39571389 | + | BMP8A | chr1 | 39976846 | + |
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Fusion Gene ORF analysis for MACF1-BMP8A |
Open reading frame (ORF) analsis of fusion genes based on Ensembl gene isoform structure. * Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser. |
ORF | Henst | Tenst | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand |
Frame-shift | ENST00000545844 | ENST00000331593 | MACF1 | chr1 | 39854330 | + | BMP8A | chr1 | 39987047 | + |
Frame-shift | ENST00000567887 | ENST00000331593 | MACF1 | chr1 | 39854330 | + | BMP8A | chr1 | 39987047 | + |
Frame-shift | ENST00000372915 | ENST00000331593 | MACF1 | chr1 | 39854330 | + | BMP8A | chr1 | 39987047 | + |
Frame-shift | ENST00000361689 | ENST00000331593 | MACF1 | chr1 | 39854330 | + | BMP8A | chr1 | 39987047 | + |
Frame-shift | ENST00000539005 | ENST00000331593 | MACF1 | chr1 | 39854330 | + | BMP8A | chr1 | 39987047 | + |
Frame-shift | ENST00000317713 | ENST00000331593 | MACF1 | chr1 | 39854330 | + | BMP8A | chr1 | 39987047 | + |
Frame-shift | ENST00000564288 | ENST00000331593 | MACF1 | chr1 | 39854330 | + | BMP8A | chr1 | 39987047 | + |
Frame-shift | ENST00000289893 | ENST00000331593 | MACF1 | chr1 | 39854330 | + | BMP8A | chr1 | 39987047 | + |
intron-3CDS | ENST00000536367 | ENST00000331593 | MACF1 | chr1 | 39854330 | + | BMP8A | chr1 | 39987047 | + |
intron-3CDS | ENST00000484793 | ENST00000331593 | MACF1 | chr1 | 39854330 | + | BMP8A | chr1 | 39987047 | + |
intron-3CDS | ENST00000602421 | ENST00000331593 | MACF1 | chr1 | 39854330 | + | BMP8A | chr1 | 39987047 | + |
intron-3CDS | ENST00000476350 | ENST00000331593 | MACF1 | chr1 | 39854330 | + | BMP8A | chr1 | 39987047 | + |
intron-3CDS | ENST00000545844 | ENST00000331593 | MACF1 | chr1 | 39571389 | + | BMP8A | chr1 | 39976846 | + |
intron-3CDS | ENST00000567887 | ENST00000331593 | MACF1 | chr1 | 39571389 | + | BMP8A | chr1 | 39976846 | + |
intron-3CDS | ENST00000372915 | ENST00000331593 | MACF1 | chr1 | 39571389 | + | BMP8A | chr1 | 39976846 | + |
intron-3CDS | ENST00000361689 | ENST00000331593 | MACF1 | chr1 | 39571389 | + | BMP8A | chr1 | 39976846 | + |
intron-3CDS | ENST00000539005 | ENST00000331593 | MACF1 | chr1 | 39571389 | + | BMP8A | chr1 | 39976846 | + |
intron-3CDS | ENST00000317713 | ENST00000331593 | MACF1 | chr1 | 39571389 | + | BMP8A | chr1 | 39976846 | + |
intron-3CDS | ENST00000564288 | ENST00000331593 | MACF1 | chr1 | 39571389 | + | BMP8A | chr1 | 39976846 | + |
intron-3CDS | ENST00000289893 | ENST00000331593 | MACF1 | chr1 | 39571389 | + | BMP8A | chr1 | 39976846 | + |
intron-3CDS | ENST00000536367 | ENST00000331593 | MACF1 | chr1 | 39571389 | + | BMP8A | chr1 | 39976846 | + |
intron-3CDS | ENST00000484793 | ENST00000331593 | MACF1 | chr1 | 39571389 | + | BMP8A | chr1 | 39976846 | + |
intron-3CDS | ENST00000602421 | ENST00000331593 | MACF1 | chr1 | 39571389 | + | BMP8A | chr1 | 39976846 | + |
intron-3CDS | ENST00000476350 | ENST00000331593 | MACF1 | chr1 | 39571389 | + | BMP8A | chr1 | 39976846 | + |
ORFfinder result based on the fusion transcript sequence of in-frame fusion genes. |
Henst | Tenst | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand | Seq length (transcript) | BP loci (transcript) | Predicted start (transcript) | Predicted stop (transcript) | Seq length (amino acids) |
DeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated. |
Henst | Tenst | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand | No-coding score | Coding score |
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Fusion Genomic Features for MACF1-BMP8A |
FusionAI prediction of the potential fusion gene breakpoint based on the pre-mature RNA sequence context (+/- 5kb of individual partner genes, total 20kb length sequence). FusionAI is a fusion gene breakpoint classifier based on convolutional neural network by comparing the fusion positive and negative sequence context of ~ 20K fusion gene data. From here, we can have the relative potentency of the 20K genomic sequence how individual sequnce will be likely used as the gene fusion breakpoints. |
Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand | 1-p | p (fusion gene breakpoint) |
Distribution of 44 human genomic features loci across 20kb length fusion breakpoint regions. We integrated a total of 44 different types of human genomic feature loci information across five big categories including virus integration sites, repeats, structural variants, chromatin states, and gene expression regulation. More details are in help page. |
Distribution of 44 human genomic features loci across 20kb length fusion breakpoint regions that are ovelapped with the top 1% feature importance score regions. More details are in help page. |
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Fusion Protein Features for MACF1-BMP8A |
Go to FGviewer for the breakpoints of :-: - FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels. |
Main function of each fusion partner protein. (from UniProt) |
Hgene | Tgene |
MACF1 | . |
FUNCTION: [Isoform 2]: F-actin-binding protein which plays a role in cross-linking actin to other cytoskeletal proteins and also binds to microtubules (PubMed:15265687, PubMed:20937854). Plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex (PubMed:20937854). Acts as a positive regulator of Wnt receptor signaling pathway and is involved in the translocation of AXIN1 and its associated complex (composed of APC, CTNNB1 and GSK3B) from the cytoplasm to the cell membrane (By similarity). Has actin-regulated ATPase activity and is essential for controlling focal adhesions (FAs) assembly and dynamics (By similarity). Interaction with CAMSAP3 at the minus ends of non-centrosomal microtubules tethers microtubules minus-ends to actin filaments, regulating focal adhesion size and cell migration (PubMed:27693509). May play role in delivery of transport vesicles containing GPI-linked proteins from the trans-Golgi network through its interaction with GOLGA4 (PubMed:15265687). Plays a key role in wound healing and epidermal cell migration (By similarity). Required for efficient upward migration of bulge cells in response to wounding and this function is primarily rooted in its ability to coordinate microtubule dynamics and polarize hair follicle stem cells (By similarity). As a regulator of actin and microtubule arrangement and stabilization, it plays an essential role in neurite outgrowth, branching and spine formation during brain development (By similarity). {ECO:0000250|UniProtKB:Q9QXZ0, ECO:0000269|PubMed:15265687, ECO:0000269|PubMed:20937854, ECO:0000269|PubMed:27693509}. | FUNCTION: Might normally function as a transcriptional repressor. EWS-fusion-proteins (EFPS) may play a role in the tumorigenic process. They may disturb gene expression by mimicking, or interfering with the normal function of CTD-POLII within the transcription initiation complex. They may also contribute to an aberrant activation of the fusion protein target genes. |
Retention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at * Minus value of BPloci means that the break pointn is located before the CDS. |
- In-frame and retained protein feature among the 13 regional features. |
Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Protein feature | Protein feature note |
- In-frame and not-retained protein feature among the 13 regional features. |
Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Protein feature | Protein feature note |
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Fusion Gene Sequence for MACF1-BMP8A |
For in-frame fusion transcripts, we provide the fusion transcript sequences and fusion amino acid sequences. To have fusion amino acid sequence, we ran ORFfinder and chose the longest ORF among the all predicted ones. |
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Fusion Gene PPI Analysis for MACF1-BMP8A |
Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in |
Protein-protein interactors with each fusion partner protein in wild-type (BIOGRID-3.4.160) |
Hgene | Hgene's interactors | Tgene | Tgene's interactors |
- Retained PPIs in in-frame fusion. |
Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Still interaction with |
- Lost PPIs in in-frame fusion. |
Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Interaction lost with |
- Retained PPIs, but lost function due to frame-shift fusion. |
Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Interaction lost with |
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Related Drugs for MACF1-BMP8A |
Drugs targeting genes involved in this fusion gene. (DrugBank Version 5.1.8 2021-05-08) |
Partner | Gene | UniProtAcc | DrugBank ID | Drug name | Drug activity | Drug type | Drug status |
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Related Diseases for MACF1-BMP8A |
Diseases associated with fusion partners. (DisGeNet 4.0) |
Partner | Gene | Disease ID | Disease name | # pubmeds | Source |
Hgene | MACF1 | C0036572 | Seizures | 2 | GENOMICS_ENGLAND |
Hgene | MACF1 | C0266463 | Lissencephaly | 2 | GENOMICS_ENGLAND |
Hgene | MACF1 | C1855677 | Brainstem dysplasia | 2 | GENOMICS_ENGLAND |
Hgene | MACF1 | C3714756 | Intellectual Disability | 2 | GENOMICS_ENGLAND |
Tgene | BMP8A | C1704436 | Peripheral Arterial Diseases | 1 | CTD_human |