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Center for Computational Systems Medicine
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Fusion Gene Summary

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Fusion Gene ORF analysis

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Fusion Genomic Features

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Fusion Protein Features

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Fusion Gene Sequence

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Fusion Gene PPI analysis

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Related Drugs

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Related Diseases

Fusion gene:NOS1AP-DCAF6 (FusionGDB2 ID:59314)

Fusion Gene Summary for NOS1AP-DCAF6

check button Fusion gene summary
Fusion gene informationFusion gene name: NOS1AP-DCAF6
Fusion gene ID: 59314
HgeneTgene
Gene symbol

NOS1AP

DCAF6

Gene ID

9722

55827

Gene namenitric oxide synthase 1 adaptor proteinDDB1 and CUL4 associated factor 6
Synonyms6330408P19Rik|CAPON1200006M05Rik|ARCAP|IQWD1|MSTP055|NRIP|PC326
Cytomap

1q23.3

1q24.2

Type of geneprotein-codingprotein-coding
Descriptioncarboxyl-terminal PDZ ligand of neuronal nitric oxide synthase proteinC-terminal PDZ domain ligand of neuronal nitric oxide synthase (CAPON)C-terminal PDZ ligand of neuronal nitric oxide synthase proteinligand of neuronal nitric oxide synthase with carDDB1- and CUL4-associated factor 6IQ motif and WD repeat-containing protein 1IQ motif and WD repeats 1androgen receptor complex-associated proteinnuclear receptor interaction protein
Modification date2020031320200313
UniProtAcc

O75052

Q58WW2

Ensembl transtripts involved in fusion geneENST00000530878, ENST00000361897, 
ENST00000493151, ENST00000454693, 
ENST00000367843, ENST00000432587, 
ENST00000312263, ENST00000367840, 
ENST00000470919, 
Fusion gene scores* DoF score15 X 9 X 8=108010 X 9 X 8=720
# samples 1810
** MAII scorelog2(18/1080*10)=-2.58496250072116
possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs).
DoF>8 and MAII<0		log2(10/720*10)=-2.84799690655495
possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs).
DoF>8 and MAII<0
Context

PubMed: NOS1AP [Title/Abstract] AND DCAF6 [Title/Abstract] AND fusion [Title/Abstract]

Most frequent breakpointNOS1AP(162124266)-DCAF6(168012263), # samples:3
Anticipated loss of major functional domain due to fusion event.NOS1AP-DCAF6 seems lost the major protein functional domain in Tgene partner, which is a essential gene due to the frame-shifted ORF.
* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

check button Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez
PartnerGeneGO IDGO termPubMed ID
HgeneNOS1AP

GO:0098974

postsynaptic actin cytoskeleton organization

26869880


check buttonFusion gene breakpoints across NOS1AP (5'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

check buttonFusion gene breakpoints across DCAF6 (3'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

check button Fusion gene information from two resources (ChiTars 5.0 and ChimerDB 4.0)
* All genome coordinats were lifted-over on hg19.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.
SourceDiseaseSampleHgeneHchrHbpHstrandTgeneTchrTbpTstrand
ChimerDB4BRCATCGA-BH-A8FY-01ANOS1APchr1

162124266

+DCAF6chr1

168012263

+
ChimerDB4BRCATCGA-BH-A8FY-01ANOS1APchr1

162124266

+DCAF6chr1

168012263

+
ChimerDB4BRCATCGA-BH-A8FY-01ANOS1APchr1

162124266

-DCAF6chr1

168012263

+


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Fusion Gene ORF analysis for NOS1AP-DCAF6

check button Open reading frame (ORF) analsis of fusion genes based on Ensembl gene isoform structure.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.
ORFHenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrand
Frame-shiftENST00000530878ENST00000367843NOS1APchr1

162124266

+DCAF6chr1

168012263

+
Frame-shiftENST00000530878ENST00000432587NOS1APchr1

162124266

+DCAF6chr1

168012263

+
Frame-shiftENST00000530878ENST00000312263NOS1APchr1

162124266

+DCAF6chr1

168012263

+
Frame-shiftENST00000530878ENST00000367840NOS1APchr1

162124266

+DCAF6chr1

168012263

+
5CDS-intronENST00000530878ENST00000470919NOS1APchr1

162124266

+DCAF6chr1

168012263

+
Frame-shiftENST00000361897ENST00000367843NOS1APchr1

162124266

+DCAF6chr1

168012263

+
Frame-shiftENST00000361897ENST00000432587NOS1APchr1

162124266

+DCAF6chr1

168012263

+
Frame-shiftENST00000361897ENST00000312263NOS1APchr1

162124266

+DCAF6chr1

168012263

+
Frame-shiftENST00000361897ENST00000367840NOS1APchr1

162124266

+DCAF6chr1

168012263

+
5CDS-intronENST00000361897ENST00000470919NOS1APchr1

162124266

+DCAF6chr1

168012263

+
intron-3CDSENST00000493151ENST00000367843NOS1APchr1

162124266

+DCAF6chr1

168012263

+
intron-3CDSENST00000493151ENST00000432587NOS1APchr1

162124266

+DCAF6chr1

168012263

+
intron-3CDSENST00000493151ENST00000312263NOS1APchr1

162124266

+DCAF6chr1

168012263

+
intron-3CDSENST00000493151ENST00000367840NOS1APchr1

162124266

+DCAF6chr1

168012263

+
intron-intronENST00000493151ENST00000470919NOS1APchr1

162124266

+DCAF6chr1

168012263

+
intron-3CDSENST00000454693ENST00000367843NOS1APchr1

162124266

+DCAF6chr1

168012263

+
intron-3CDSENST00000454693ENST00000432587NOS1APchr1

162124266

+DCAF6chr1

168012263

+
intron-3CDSENST00000454693ENST00000312263NOS1APchr1

162124266

+DCAF6chr1

168012263

+
intron-3CDSENST00000454693ENST00000367840NOS1APchr1

162124266

+DCAF6chr1

168012263

+
intron-intronENST00000454693ENST00000470919NOS1APchr1

162124266

+DCAF6chr1

168012263

+

check buttonORFfinder result based on the fusion transcript sequence of in-frame fusion genes.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandSeq length
(transcript)		BP loci
(transcript)		Predicted start
(transcript)		Predicted stop
(transcript)		Seq length
(amino acids)

check buttonDeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandNo-coding scoreCoding score

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Fusion Genomic Features for NOS1AP-DCAF6


check buttonFusionAI prediction of the potential fusion gene breakpoint based on the pre-mature RNA sequence context (+/- 5kb of individual partner genes, total 20kb length sequence). FusionAI is a fusion gene breakpoint classifier based on convolutional neural network by comparing the fusion positive and negative sequence context of ~ 20K fusion gene data. From here, we can have the relative potentency of the 20K genomic sequence how individual sequnce will be likely used as the gene fusion breakpoints.
HgeneHchrHbpHstrandTgeneTchrTbpTstrand1-pp (fusion gene breakpoint)
NOS1APchr1162124266+DCAF6chr1168012262+0.0015153430.9984847
NOS1APchr1162124266+DCAF6chr1168012262+0.0015153430.9984847

check buttonDistribution of 44 human genomic features loci across 20kb length fusion breakpoint regions. We integrated a total of 44 different types of human genomic feature loci information across five big categories including virus integration sites, repeats, structural variants, chromatin states, and gene expression regulation. More details are in help page.

check buttonDistribution of 44 human genomic features loci across 20kb length fusion breakpoint regions that are ovelapped with the top 1% feature importance score regions. More details are in help page.

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Fusion Protein Features for NOS1AP-DCAF6


check button Go to

FGviewer for the breakpoints of :-:

.
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
FGviewer

check buttonMain function of each fusion partner protein. (from UniProt)
HgeneTgene
NOS1AP

O75052

DCAF6

Q58WW2

FUNCTION: Adapter protein involved in neuronal nitric-oxide (NO) synthesis regulation via its association with nNOS/NOS1. The complex formed with NOS1 and synapsins is necessary for specific NO and synapsin functions at a presynaptic level. Mediates an indirect interaction between NOS1 and RASD1 leading to enhance the ability of NOS1 to activate RASD1. Competes with DLG4 for interaction with NOS1, possibly affecting NOS1 activity by regulating the interaction between NOS1 and DLG4 (By similarity). {ECO:0000250}.FUNCTION: Ligand-dependent coactivator of nuclear receptors. Enhance transcriptional activity of the nuclear receptors NR3C1 and AR. May function as a substrate receptor for CUL4-DDB1 E3 ubiquitin-protein ligase complex. {ECO:0000269|PubMed:15784617, ECO:0000269|PubMed:16949367, ECO:0000269|PubMed:16964240}.

check buttonRetention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at

download page


* Minus value of BPloci means that the break pointn is located before the CDS.
- In-frame and retained protein feature among the 13 regional features.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note

- In-frame and not-retained protein feature among the 13 regional features.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note


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Fusion Gene Sequence for NOS1AP-DCAF6


check button For in-frame fusion transcripts, we provide the fusion transcript sequences and fusion amino acid sequences. To have fusion amino acid sequence, we ran ORFfinder and chose the longest ORF among the all predicted ones.

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Fusion Gene PPI Analysis for NOS1AP-DCAF6


check button Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in

ChiPPI page.


check button Protein-protein interactors with each fusion partner protein in wild-type (BIOGRID-3.4.160)
HgeneHgene's interactorsTgeneTgene's interactors


check button - Retained PPIs in in-frame fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenStill interaction with


check button - Lost PPIs in in-frame fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenInteraction lost with


check button - Retained PPIs, but lost function due to frame-shift fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenInteraction lost with


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Related Drugs for NOS1AP-DCAF6


check button Drugs targeting genes involved in this fusion gene.
(DrugBank Version 5.1.8 2021-05-08)
PartnerGeneUniProtAccDrugBank IDDrug nameDrug activityDrug typeDrug status

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Related Diseases for NOS1AP-DCAF6


check button Diseases associated with fusion partners.
(DisGeNet 4.0)
PartnerGeneDisease IDDisease name# pubmedsSource
HgeneNOS1APC0035828Romano-Ward Syndrome2ORPHANET
HgeneNOS1APC0036341Schizophrenia2PSYGENET
TgeneDCAF6C0033578Prostatic Neoplasms1CTD_human
TgeneDCAF6C0376358Malignant neoplasm of prostate1CTD_human