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Center for Computational Systems Medicine
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Fusion Gene Summary

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Fusion Gene ORF analysis

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Fusion Genomic Features

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Fusion Protein Features

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Fusion Gene Sequence

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Fusion Gene PPI analysis

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Related Drugs

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Related Diseases

Fusion gene:NUP35-NCKAP1 (FusionGDB2 ID:60673)

Fusion Gene Summary for NUP35-NCKAP1

check button Fusion gene summary
Fusion gene informationFusion gene name: NUP35-NCKAP1
Fusion gene ID: 60673
HgeneTgene
Gene symbol

NUP35

NCKAP1

Gene ID

129401

10787

Gene namenucleoporin 35NCK associated protein 1
SynonymsMP-44|MP44|NP44|NUP53HEM2|NAP1|NAP125|p125Nap1
Cytomap

2q32.1

2q32.1

Type of geneprotein-codingprotein-coding
Descriptionnucleoporin NUP35mitotic phosphoprotein 44nuclear pore complex protein Nup53nucleoporin 35kDanucleoporin NUP53nck-associated protein 1membrane-associated protein HEM-2
Modification date2020031320200327
UniProtAcc.

P55160

Ensembl transtripts involved in fusion geneENST00000409798, ENST00000295119, 
ENST00000541912, ENST00000497330, 
ENST00000361354, ENST00000360982, 
ENST00000478449, 
Fusion gene scores* DoF score4 X 4 X 4=6414 X 14 X 8=1568
# samples 417
** MAII scorelog2(4/64*10)=-0.678071905112638
possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs).
DoF>8 and MAII<0		log2(17/1568*10)=-3.20531890797751
possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs).
DoF>8 and MAII<0
Context

PubMed: NUP35 [Title/Abstract] AND NCKAP1 [Title/Abstract] AND fusion [Title/Abstract]

Most frequent breakpointNUP35(183989225)-NCKAP1(183853914), # samples:3
NCKAP1(183859566)-NUP35(183983416), # samples:1
Anticipated loss of major functional domain due to fusion event.
* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

check button Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez
PartnerGeneGO IDGO termPubMed ID
TgeneNCKAP1

GO:0016601

Rac protein signal transduction

21107423

TgeneNCKAP1

GO:2000601

positive regulation of Arp2/3 complex-mediated actin nucleation

21107423


check buttonFusion gene breakpoints across NUP35 (5'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

check buttonFusion gene breakpoints across NCKAP1 (3'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

check button Fusion gene information from two resources (ChiTars 5.0 and ChimerDB 4.0)
* All genome coordinats were lifted-over on hg19.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.
SourceDiseaseSampleHgeneHchrHbpHstrandTgeneTchrTbpTstrand
ChimerDB4LUSCTCGA-43-8115-01ANUP35chr2

183989225

+NCKAP1chr2

183853914

-
ChimerDB4LUSCTCGA-43-8115NUP35chr2

183989225

+NCKAP1chr2

183853914

-
ChimerDB4LUSCTCGA-43-8115-01ANUP35chr2

183989225

-NCKAP1chr2

183853914

-


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Fusion Gene ORF analysis for NUP35-NCKAP1

check button Open reading frame (ORF) analsis of fusion genes based on Ensembl gene isoform structure.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.
ORFHenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrand
intron-3CDSENST00000409798ENST00000361354NUP35chr2

183989225

+NCKAP1chr2

183853914

-
intron-3CDSENST00000409798ENST00000360982NUP35chr2

183989225

+NCKAP1chr2

183853914

-
intron-intronENST00000409798ENST00000478449NUP35chr2

183989225

+NCKAP1chr2

183853914

-
In-frameENST00000295119ENST00000361354NUP35chr2

183989225

+NCKAP1chr2

183853914

-
In-frameENST00000295119ENST00000360982NUP35chr2

183989225

+NCKAP1chr2

183853914

-
5CDS-intronENST00000295119ENST00000478449NUP35chr2

183989225

+NCKAP1chr2

183853914

-
5UTR-3CDSENST00000541912ENST00000361354NUP35chr2

183989225

+NCKAP1chr2

183853914

-
5UTR-3CDSENST00000541912ENST00000360982NUP35chr2

183989225

+NCKAP1chr2

183853914

-
5UTR-intronENST00000541912ENST00000478449NUP35chr2

183989225

+NCKAP1chr2

183853914

-
3UTR-3CDSENST00000497330ENST00000361354NUP35chr2

183989225

+NCKAP1chr2

183853914

-
3UTR-3CDSENST00000497330ENST00000360982NUP35chr2

183989225

+NCKAP1chr2

183853914

-
3UTR-intronENST00000497330ENST00000478449NUP35chr2

183989225

+NCKAP1chr2

183853914

-

check buttonORFfinder result based on the fusion transcript sequence of in-frame fusion genes.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandSeq length
(transcript)		BP loci
(transcript)		Predicted start
(transcript)		Predicted stop
(transcript)		Seq length
(amino acids)
ENST00000295119NUP35chr2183989225+ENST00000361354NCKAP1chr2183853914-19327143762739887
ENST00000295119NUP35chr2183989225+ENST00000360982NCKAP1chr2183853914-3565143762739887

check buttonDeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandNo-coding scoreCoding score
ENST00000295119ENST00000361354NUP35chr2183989225+NCKAP1chr2183853914-0.0001351130.9998648
ENST00000295119ENST00000360982NUP35chr2183989225+NCKAP1chr2183853914-0.000308440.9996916

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Fusion Genomic Features for NUP35-NCKAP1


check buttonFusionAI prediction of the potential fusion gene breakpoint based on the pre-mature RNA sequence context (+/- 5kb of individual partner genes, total 20kb length sequence). FusionAI is a fusion gene breakpoint classifier based on convolutional neural network by comparing the fusion positive and negative sequence context of ~ 20K fusion gene data. From here, we can have the relative potentency of the 20K genomic sequence how individual sequnce will be likely used as the gene fusion breakpoints.
HgeneHchrHbpHstrandTgeneTchrTbpTstrand1-pp (fusion gene breakpoint)

check buttonDistribution of 44 human genomic features loci across 20kb length fusion breakpoint regions. We integrated a total of 44 different types of human genomic feature loci information across five big categories including virus integration sites, repeats, structural variants, chromatin states, and gene expression regulation. More details are in help page.

check buttonDistribution of 44 human genomic features loci across 20kb length fusion breakpoint regions that are ovelapped with the top 1% feature importance score regions. More details are in help page.

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Fusion Protein Features for NUP35-NCKAP1


check button Go to

FGviewer for the breakpoints of chr2:183989225-chr2:183853914

.
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
FGviewer

check buttonMain function of each fusion partner protein. (from UniProt)
HgeneTgene
.NCKAP1

P55160

FUNCTION: Might normally function as a transcriptional repressor. EWS-fusion-proteins (EFPS) may play a role in the tumorigenic process. They may disturb gene expression by mimicking, or interfering with the normal function of CTD-POLII within the transcription initiation complex. They may also contribute to an aberrant activation of the fusion protein target genes.FUNCTION: Essential hematopoietic-specific regulator of the actin cytoskeleton (Probable). Controls lymphocyte development, activation, proliferation and homeostasis, erythrocyte membrane stability, as well as phagocytosis and migration by neutrophils and macrophages (PubMed:16417406, PubMed:17696648). Component of the WAVE2 complex which signals downstream of RAC to stimulate F-actin polymerization. Required for stabilization and/or translation of the WAVE2 complex proteins in hematopoietic cells (By similarity). Exhibits complex cycles of activation and inhibition to generate waves of propagating the assembly with actin (PubMed:16417406). Also involved in mechanisms WAVE-independent to regulate myosin and actin polymerization during neutrophil chemotaxis (PubMed:17696648). {ECO:0000250|UniProtKB:Q8K1X4, ECO:0000269|PubMed:16417406, ECO:0000269|PubMed:17696648, ECO:0000303|PubMed:20969869}.

check buttonRetention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at

download page


* Minus value of BPloci means that the break pointn is located before the CDS.
- In-frame and retained protein feature among the 13 regional features.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note
TgeneNCKAP1chr2:183989225chr2:183853914ENST00000360982832995_1015269.33333333333331135.0TransmembraneHelical
TgeneNCKAP1chr2:183989225chr2:183853914ENST00000361354731995_1015263.33333333333331129.0TransmembraneHelical

- In-frame and not-retained protein feature among the 13 regional features.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note
HgeneNUP35chr2:183989225chr2:183853914ENST00000295119+19170_25013.333333333333334327.0DomainRRM Nup35-type


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Fusion Gene Sequence for NUP35-NCKAP1


check button For in-frame fusion transcripts, we provide the fusion transcript sequences and fusion amino acid sequences. To have fusion amino acid sequence, we ran ORFfinder and chose the longest ORF among the all predicted ones.
>In-frame_ENST00000295119_ENST00000361354_TCGA-43-8115-01A_NUP35_chr2_183989225_+_NCKAP1_chr2_183853914_length(transcript)=19327nt_BP=143nt
ACGCAACCCCATAAGGTAGCACGCCGTTACCCGTGGGGAGCGTTTCCGCCATTTTTGAAAATTAATTGGGAAGGTACTGGTTTTAAGTGT
AGTTGCCGACGCAATGGCAGCCTTTGCAGTGGAACCTCAGGGGCCCGCGTTAGTTGGCTTTATTTTGTGCCATGGGATCCTAAATACTGA
CGCTACAGCACTGAACCTTTGGAAACTAGCTCTTCAAAGTAGCTCTTGCCTCTCTCTCTTTCGGGATGAAGTTTTCCACATTCACAAAGC
TGCAGAAGACTTATTTGTAAACATACGAGGCTATAATAAACGTATTAATGACATAAGAGAATGCAAGGAGGCAGCCGTGTCACATGCTGG
TTCAATGCACAGAGAAAGACGCAAGTTTTTAAGATCTGCACTGAAGGAATTGGCTACTGTCCTCTCTGATCAACCTGGATTGCTAGGTCC
CAAGGCACTTTTTGTTTTTATGGCATTATCCTTTGCCCGTGATGAAATCATCTGGCTACTTCGTCATGCAGATAACATGCCAAAGAAGAG
TGCAGACGACTTTATAGATAAGCACATTGCTGAATTAATATTTTACATGGAAGAACTTAGAGCACATGTGAGGAAATACGGACCTGTAAT
GCAGAGGTATTACGTGCAGTACCTTTCTGGCTTTGATGCTGTTGTCCTCAATGAACTCGTGCAGAATCTTTCTGTTTGCCCTGAAGATGA
ATCAATCATCATGTCCTCTTTTGTTAACACTATGACTTCCCTAAGTGTAAAACAAGTTGAAGATGGGGAAGTATTTGATTTCAGAGGAAT
GAGATTAGATTGGTTTAGGTTACAGGCATATACTAGTGTCTCAAAGGCTTCACTTGGCCTTGCAGATCACAGAGAACTTGGAAAGATGAT
GAATACAATAATTTTTCATACAAAAATGGTAGATTCCTTGGTGGAAATGTTGGTGGAAACATCAGATCTCTCCATATTTTGTTTTTATAG
TCGTGCTTTTGAGAAGATGTTTCAACAGTGTTTGGAGTTACCCTCTCAATCAAGATACTCAATTGCATTTCCACTACTTTGCACTCATTT
TATGAGTTGCACGCATGAACTATGTCCAGAAGAGCGACATCATATTGGAGATCGCAGTCTTTCCTTATGTAATATGTTCCTAGATGAAAT
GGCCAAACAAGCTCGAAATCTCATCACTGATATTTGCACAGAACAGTGTACCCTTAGTGACCAGTTGCTACCCAAGCATTGTGCCAAAAC
TATCAGTCAAGCAGTGAATAAGAAATCAAAAAAGCAGACTGGTAAGAAAGGGGAACCTGAAAGGGAGAAACCAGGTGTTGAGAGCATGAG
GAAAAACAGGCTGGTTGTGACCAACCTTGATAAATTGCACACTGCACTTTCTGAGTTATGCTTCTCTATAAATTATGTACCAAACATGGT
GGTATGGGAACATACCTTTACCCCACGAGAATATTTGACTTCTCATCTGGAAATACGCTTTACCAAGTCAATTGTTGGGATGACTATGTA
TAATCAAGCCACACAGGAAATTGCAAAACCTTCAGAACTTCTAACAAGTGTAAGAGCATACATGACCGTACTCCAGTCAATAGAAAACTA
TGTGCAGATTGATATTACAAGAGTATTTAATAATGTGCTTCTTCAACAAACACAACATTTAGACAGTCATGGAGAGCCAACCATTACAAG
TCTATACACAAATTGGTATTTGGAAACTTTGTTACGACAAGTCAGCAATGGCCATATAGCATATTTTCCTGCAATGAAAGCGTTTGTGAA
CTTACCTACAGAAAATGAATTAACATTCAATGCAGAGGAATATTCTGACATATCAGAAATGAGGTCATTATCAGAACTACTAGGCCCATA
TGGTATGAAGTTTCTAAGTGAAAGCCTTATGTGGCATATTTCATCACAAGTTGCTGAACTTAAGAAACTTGTGGTGGAGAATGTTGATGT
GTTAACACAAATGAGGACCAGCTTTGACAAACCAGACCAGATGGCTGCACTGTTTAAAAGATTATCATCTGTTGACAGTGTCTTGAAGAG
GATGACAATAATTGGTGTAATTTTATCCTTCCGATCATTGGCACAAGAAGCACTTAGAGATGTCTTATCCTACCACATTCCTTTTCTTGT
AAGTTCAATTGAAGATTTTAAGGATCACATTCCAAGGGAAACTGATATGAAGGTTGCAATGAATGTGTATGAGTTATCATCAGCTGCCGG
ATTACCTTGTGAGATTGATCCTGCATTGGTCGTAGCTCTTTCTTCACAAAAATCGGAAAACATTAGTCCAGAAGAAGAGTATAAAATTGC
CTGCCTTCTCATGGTGTTTGTGGCAGTTTCTTTGCCAACACTGGCCAGTAATGTGATGTCTCAGTACAGCCCTGCTATAGAAGGGCATTG
CAACAACATACATTGCTTGGCCAAAGCCATCAACCAGATTGCTGCAGCTTTGTTTACAATTCACAAAGGAAGCATTGAAGACCGTCTTAA
AGAATTTCTGGCGCTTGCATCCTCCAGTCTACTGAAAATTGGCCAGGAGACAGATAAAACTACAACAAGAAATAGAGAATCTGTTTATTT
ACTGCTAGATATGATTGTACAAGAATCTCCATTCCTTACAATGGATCTTTTGGAATCTTGTTTTCCTTATGTCTTGCTGAGAAATGCATA
CCATGCTGTCTACAAACAAAGTGTTACATCTTCTGCATAAAATTACCTACTTAATCAAGATAAGCACGCATTTTTGTTGCCTTGGTTTTA
CCTGTAGACTGTGGAACTATTTTACCTTAAGACCTGAAAAAGTTTTGTGGATTATAAATTTCTTTCATACGGTTGTATTTTCTGATCATT
GGTTTCTTAATATGGTTGTACTACAGTATACTTGGTTGATTTAGGTTGCACATTCACTGAATTCACTGAGATTATTCCTATAATTTTAAA
GTATCATTTATTTGAAAAACATACATTATCAACATGTTTTTGATATTTGATAATGAAAAAAATCTTTGCTTGTTTATTTCTGAAAAAGAA
CTGTATTTAGTGATTATTTTAGATAGTGATATTATAGTATTCATCTGTGTGTAAATTATTTCATATAGGGAAGAGTTCTGATCTGTACCT
ATGGTTCTTATTGAAAACAACATTGGATGTGCATTTCTGTGATGTTATGAATACATTTCTACTTTATTTTGAAACATTTGCCAAACTAAA
TACTGTAACACTGTATAACATTTAAAAATGTTAAAGAACTGCTTAGTATTAGAAGCAGATCATTTCCCAAAATTCTAAGAGCAGCAGCAT
ATGTTGTTGCTTGTATAAAGCCTAGCGATAATTTTTAGACTAACTTCCATGGTGCCCTGTTGGCATTAGCACTACCATTGTACCCTGCTG
TATAATAAACAATCTTAGACATTTATCAACTGTTGATACAAATGTTAGTCCCTAACCACTTTTTATATATGTTTTAAATTTTTGAAATTC
AAGTGTACCTGCCATAACATAAAATAAACACTAGACTGTATCACACTTCGATTGATTTCTTTAAGATCCTTGGATATTCGTTCAATAATA
ATAAAGTTTTTGTTTAAACTTAGTTTGAAATATAAAAAATAATGGTTTAGGAATGTCTAAGTACAGCATTCGATACCAGGTCAGCTATTA
TATGATTTGTTTAAATGTTTTAAAAAACATTTTATTGAAAAAAATACCAAAATTAGAAAATAAAATTACACAATACTTCTTAAAAATTAA
GAACTTAAAGAAAACTTGTATGCTGTCAACTTGCTCATTTTATCTGTAACCAAATAGAGTTATTTAAAAGAATAGGGAAAAAAAATTGAG
TGATCATGGGACTAGTGAGAATGTTGAGAACTGCTGCATTAGTCAGTAATGAATGGGAAAGACATAAAAGGGCCTATTGCTTAATAGTTG
GCATGGAGGGCAAAGCAGCTCATTTAGTTTGAAAACTAACCCAATGTTTTGCTCTTTGATTGGCATTGTTGAGGCTGTTTCTCAGCAGCT
TTGTATATGAATATCATAAAGAGCTTCTGTTCACAGCTTCTTATGTATACACTGGTAACTTGTGTTAAGATAAAAGAAGGCTGCTGTGAA
CTCAGTTATAATGTTTTTCTTTCTTCAAACATAGAGAGTAGAATGAAATTGAAGTACTATGAGATTATGCTATTATTTTGTGTATGATTT
GTCCAGCCTATATTTAAAGTAGGGAGTAGTTGTCACTATCTGTCGAAAAATTGTACTTGTTCCATAAAGATTTTTTAAAAAGGGAAATTT
TAAAATGGCATAGTTCAGACCTGAAATAGTCTGATTTACACAGTGAGCAGTCAAAACTAAGATTTAATGCCGTTTGTTTTGAAATATTAG
GATCTTAGAGAGGTAATAACTTTGTTGGCAGTGATCTCTGGAGAGGTAAAGTAGGAATTTGGATATGTACACTTTGGAATCATATGTGAA
AGGGCATAAGGATGAAAATAAGGCATTCTCTACCACTGCTAGGCTGAGAAAAAAATCGCTCCTCATAGTCACCTGAAGTTTTTGTCCAAA
TTTTTTCTCATACAGCAAAGGCGGGGGAGGAGGCGTGGCAAAGGTAACTTGGAGGAAAAAGTTAAAAGTAAATTGACAACTACTATTTTT
AATATCTTGTTTGTTTTATTTTCTTCTAAACTTACACAACAACATACTATTTTTGTTGTTTTCTCTACATTAATGTGATCATACCATACA
TACTGGCCTGTAATCTGCTTATTTCACTCAGTAGTAGAAAAAATGTCTTTAATATTCTTTTTAATACCTTTATAGATACCATAACTAATT
TAACAATTTCATGGCCAGGCGTAGTGGCACACGCCTATTTCAGCACTTTGGGAGGCCAAGGCGGATGGATCACTTGAGGTCAGGAGTTCA
ACACCAGCCTGGCCAACATGGCAAAACCCTGACACTACTAAAAATACAAAAATTACCCAGGTATGATGGTGCACACCTATCATCCTGGCT
ACATGGGAGGCTGAGGTGGAAGAATTGTTTGAACCCGGAAGGAGGAGGTTGCTGTGAGCTGAGATCACACCACTGCACTCCAGCCTGGAT
GACAGAATGAGACTGTCTCAAAAAAAAAAAGAAAATACTCATTTGGATAGATTGGATAGATTTTTAGACAATTTCTTTTCATTATTATAA
ATGTATATCTATATCTTGGTAAAGTTGACCTGTTATTTTTTTAGAATAATTTTTCAGAAGTAAAATTGCTTCCTTTCTGCTGCTTTTTCA
TTCACTTATTTAAGCAAGTATTTTTAGATGCCTGCATGTAATGGTGAGCAAAAAGGTGACCCTTTTATAGTTGAGCTTGGATTTTAGTGA
TGGTATACAATAAACAAACCAGCTTCTTGAGACGGAGTTTCACTCTTGTTGCCCAGGAAATGGGCAATGGCCTGATCTCGGCTCACTGTA
ACCTCCGCCTCCCGGGTTCAAGCAATTCTCCCATCTCAGCCTCCTGAGTAGCTGAGATTACAGGCGCCCGCCACTACGCCCAGTTAATTT
TTGGTATTTTTAGTAGAGATGGGGTTTTACCATGTTGGCCAGGCTGGTCTCGAACTCCTGACCTCAGGTGATCCACCCACCTCAGCCTCC
CAAAGTGCTGGGATTACAGGCATGAGCCACCACGCCCAGCCATGAGCAAACTTCTGATGGTGACTGGAAAGTATACAGGATAATGTGGGG
AGGCAGTGGTGAAACTACTTTAGAAGAAAGTGTTAGAAGAAAGTGTCATCTGTGTAAGAGAAAACATTTGAGCTGGTACGTGAAGGATTA
GAAGGAACCACAAGTGGGCTGAGGGGAGGAATAATAAAGGCAAAGACCCAGAAGGCAAGCACAAACTTGGCATGTTTGAGAAGTTAGAAG
TGTGTCATCTGTGTCTCTGTCATGAGGAGTGCTCAAGTTCCCTCTAGGAGCCTTATGGGCTATCTAGCTCTTTTTCTCTTCTGATCATGT
GCTTGCTTGCTTTCTTCTTTGCTTATATAGCAGAGTTCACAAATTAAATCTAATGTAGGCTGGTTAATAAAGGAAATGAACATGAATTCA
CTGATAGGCTCTTGCATTAATGTGAAAATGTAAAGTCTGTTGTACACCATCAGGCCTTCTAAATGAAGCCCAAATTCTGTAAGGTAGTGA
ATTGTAACCTACTGATTAGTACATGATCCATGCCCATTCTGTTATAAGGACTTGCCTTTCTGAGACTTCACCTGTATTATTCCTAAGACT
TTTGTGAATAATCCTTAAAGTAGTGACTCCTTACATCTTTCTTTCATTTCTTCTTTGTAGTCACTTGAAATGACAGCATGAACCATCATT
TCATTTGAAGGCCTGAGCCTGAATTTTCAAATAGACAAAATGCTCTCTTTCTCCGAAGATTTGCCATGACCTCTGCCTTTGGGAAAAAAT
ATTTGAGATTTTAAAATTTTTATTCTGTAGTACAAAAATTATGTTTTTCGTTAATTGGTCCTGTGGAATTGGAAGGAAATTTAATATTTA
TTGACTCTACTTTGGTATTATTTTCAGAGTGAGAAGTAACTTTAAGTGTATTAAAATTTCATTCATTTTTGCATGAACCAAACTTTCCCT
ACACATCGCTGTCCTTGACTGTCCAGAGTAAACATGTCTAACTCTGGAATGTTTCATTTCCAATAAAAGTATTCAGCCTGTCATTTTGGG
CCTAAGTAAAATGGGATAAATTAGCAATCTGAAGTTAGGATCCTAGCCACAGAGGGCCATGTCTCATTTCTGGTTTAAGTGCACCCCTCA
TCCCCAGCCCTTACCTTCTTCTTTCTCTTAAGGATTGAGACAATCCTCTTGGTGCTCTTGGTGCAGGTGCATCTTGGTCACCTCTTACAT
GAGGGCCACAAACTTGGTAAATGGGTGGCCTGGAGATGGGGTTGTACTAGGTATGTGACCTTGGTGATTGCCTTGTCTCAGTTGTAGTAT
ATGGCTGAGTCACATTCATAGTACACTACATACACATACTGACCAAATGCCAATGGCTAAGGCTTGAAATTATTTCACCAAGACCTAAAC
TGAAGATTACCATCATAAAATAAGCTTCTAGAATCCATGAATAAGTAGGAAATCATATTTAAAATTTACTGTAATTTTATTAAAGCTCAT
TTTTTAAATTTAATGCAGTGCAGAGTCATTAATTTCTCAGCAGTATCAGGTGCTAAATTGCTCACTCATTAAACTACTAATTTAAGATAG
TTATTTTAAATTATAGAACAGGAAGCAACTTACGAGGTTGCAACCAAATTATATTTATTTTTTCTCCATTAGTAACTGACTAAAAGTGTT
TTCTTCAACTCTGGGATTTGAAGATTTTGTGGTCAGTGGTGATATAATTTCACTGAAGCGTGCTCACAGACTAAAACTGTGCACTACTCA
GTGATGCGTCCTGAATGTTTTCAGAGACAAACCTAAGATAATGTTGCCCTTGTTTACTTTCATTATTTCTGATTTTTTTTTTATGCATGT
GTTTGCTGCATCACACAATTACTTAAGAAGTTGTCCCTAACCTTTGTGCTGTGGTCTGAATGTTTGTGTCCCCCTGGCCACAAAATTCAT
ATGTTGAAATCTGATCATCAGTGTGACAGGATGTGGGGCCTTTGGGAGGTGATTAGGTCATGAGAGTGGAGCCTTCATGAATGGGATTAA
TGCCCTCGTAAAAGGGGCCCCAGACAGCCGACTTGCCCCAACCACCACGTGAGGACAAAGCAAGAAGCCACTGTTCATGAACCAGAAAGC
AGACCCTCCACAAACACCAAATATGCCAGCACACTGATTGGCTTCCCAGCCTCCAGAACTATAAGCAAGAAGTTTTTTTATAGTAACAAA
TTTATTGTTCACAGTTCTTGCTTATAAACTTTATAAACCAGACTAAGGCATTTCGTTATAGCAGCCCACACGGACTCATATTTTGGTTCT
AGAAATGTGACACTGGAATGATGTGGCTCTCAAGATGACAGTGTTGCTTCTGAATGTCTATGTTCATGTCATCTTTAATTGCATGAACAC
CAGTTTTAGCATCTTCCCTTGAGTAGAAAGAGATTCTCTACAGCAAGTATTACGTAAGAAGTACTGGTGGCCAGGCACAGTGGTTCACGC
CTGTAATCCCAACACTTTGAGAGGCGGAGCCAGAGGTATCATTTGAGCCTAGAAGTTCGAGAGCAGCCTGGGCAATATGTCTAAACCCCA
TCTCTACAAAAAAAATACAAAAATGAAAAAGCCGGGTGTGATTGTGTACACCTGTAGTCCCAGCAACTTGAGAAGCTGAGGTGGCAGGAT
TGCTTGAGCCCAAGGGGAGGGTTGGCGGCAGTGGGTGGTTCGAGGCTGCAGTGAGCCATGATTATACCAGTGCGCTCCAGCCTAGGTGAC
AGAGCAAGAGCCGGTCTCGATAATAATTAAGGCCAGACGCGGTGGCTCACTCCTGTGATCCCAGCACTTTGGGAGGCCAAGGTGGATGGA
TCACCTGAGGTCAAGAGATTGAGACCAACCTGGCCAACATGGTGAAACCCTGTCTCTACTAAAAATACAAAAATTAGCTGGGCTTTGTGG
CTCGCGCCTGTAATCCCAGCTACTCAGGAGGCTGAGGCGGGAGAATCGCTTGAACCCAGGAGGCGGAGATTGCAGTGAGCCGAGATCATG
CCACTGCACTCCAGCCTGGGCAACAGAACAAGACTCTGTCTCAAAAAAAAAAAAAATTATAATACATTAAAAAAAAAAAGCACTTGCATT
CTGAAGGCATATTCACCCTTGAACTGATAGCATTCAATGTATAAAACACATGAGTTTCACTCCAAGTTACATATAGAATGTTTATCTTTA
TTTGAGAGGAAATATCCAGTTAATAATCAGAATGTCATACTAATTAGGTATGACCATAACTTACGTTAGTCACATGGTCATTGATTGCAT
GCAGATTCTGGCTGCCAGAAAGCACATTGAAGTAGGTAGTCATTTAACCATTTAACTGTTAGATCTTGCAAAGGTTGAAGCTGAAGCTGA
GGAGCAGGTAGTACACAACTTTACAGGTTAATGCTGAATGTCAGTTCATTCTGAATTCTTACCTTCAGCCAAAAGGTACAAGGGTCTTTG
CAGTCTGAAGCAAGGCCCAGATGGCAGAACTTGTGATGGCTAGGAGGGGAAAATTTGACCTTAACTGGTAGTTGTGGTTTTATCAAGCCA
AAAATGCTGACTAGAATAATAATGAGAGCTAATGTTTATTGAAAGCCCATTGTGTACCAGATACTCTGTTTTCTCTAATTCAAACCTCTA
AATATTGCTCTTTTAGTCTTCACAGTAATCCTATATCATGATGACCCCCATTTATTGATATGGAAACTGAAGCTCAGGAAAGACAAAGCA
ATTTTTACAAACTCACACTACAAGGAAGTAGCAGAGGCCAACATTTAAATCTGGCCCCAGAGTTGTGTTCTTCTCATTAAACTGTGTCTC
AGATCTCTCAAATGTGAGTGTGGACTTTTCTAACCAATAGCATTTTTTAATTAATGTTTTAAAAATTTGAATAGCTTTTAGGGTACAGTG
GGTTTTTGTTTACATGGATGAATCATACATTGGTGAAGTCTGAGGTTTTAGTGTACCTGTCACCCAGGTAGTATGCATTGTACCCAATGT
GTAGTTTTTTATCCCTAGACCCGTTCCCATCCTCCCACTTCTGAGTCTCCAAAGTCCATGATATCACTCTGTATGCCTTTGCATACACAT
AGCTTAGCTCCCACTTACAAGTGAGAACATACGGTATTTGGTTTTCTGTTCCTGAGTTACTTTGCTTAGAATAATGGCCTCTGGCTCCAT
CATAGTTGCTGCAAAAAGACATTGTTTCTTTTTTTATGGCTGAACAGTATTCCGTGGTGTGTATATATACCACACTTTCTTTGTCCACTC
ATTGGTCAGTGGGCACTTAGGTTGGTTCCATATCTTTGCAGTTATGAATTGTGCTGCAATAAATATACACATGCAGATTTTTTTTGATAT
ATTGACTTCCTTTGGGTAGATACCCAGTAGTAGGATTGCTGGATCAAATTCTATCCAGTAGCATTTGAAACTGCTTTACATTAACAATGA
GGGACAAAAAAAATAACAATGAGGGCTTACATTATGCTACTCTTGATACTAGACATTTTCATATCCATTACTTCATTTAAACTCCACAAC
CAAATTACTCAATAGGGATTGTTTTCTTTATTTTTGTAAATGAATGTGGAAACCAGCTCATAAAACTTTAAATTCCTTGCCCCAGTGTCA
TAAAATTACTGAGTTTGAGTGGCAGTGCTGAGATTAAAACCTAAGGCTCTAGGATTTCCAGCCCATATTTTTTCTACTGTATCATGTTCT
TTCCCAAAGCACTAAAAAACATTTCCTGGGTAAATGACTGTGGGCTTGGGGACACTATGCCCACTTTCTGGCCTTCATGGGTCTAAGTTG
CTTCTTTGCTTCTTCACTTTTTCTCTCTGATCCTGCTCAGATTAATCTCTAGTAAATCAGATTTCTTTCCTAGGCTGTCTTATCCCACAT
ATATAGTAGATAATATGTCACATATTTGTGAATGTTAAAAATATTTGTTTTAGTTTTAAATCTCAAGCTCACACAGTACTTACTCTCTAT
GTTAACAAAAATGGTTTTTACTATTTTCTGAAGGTAACGCAGCTGTTGAAGTGAGATCCTACACTGGAACATACAACACTTGGTCTAATA
TTAAAGTTCGTGCCAAGAGCTCTCTAAATGTGATTGATGGATTAATTCTGCCTTTATGAAAGATGAACTTATTTTCAAGATAATAGCTAA
TTCTTGAATGGGCAACCTATTATGTATCAATGATTGTAATGATGGAGCATTTTAGTCATTTGTAGCTGGAAGAGCCTTAGAGATTATCTG
GTTCAACCTATTCAAGAGTTAGGTAATTTAACCAAATTAACATATGTGGCGATGTGCTCATATAGTGTTCCTTCCACTGTGCTGTGTTGC
CGCATATATGCTGATCTTATGTTTATGCAGTAATAGTCTTCATAATGATAAACAGCTACTGGTTCCTGGTAGGATTTTGGACGTGGATAT
GACTTGAAGTATGATGCCACAGCCTCAAACAAATCTGGGGTTTGAATCCCAGCTCTGTCACTTATGGACAAATTACTTAACTTCTCCAAG
TTTGTTTCCCTAAGTGTAGAATGTGGATAATATCTACTTTAAAGGGCTATTGTAAGCATTAAAATAAGATCATGCGTATAAATTGATGAG
ATGGTGGGGAAGAAGAGGCTACCACAAAGTAGGCATTCAGTGTAAATGGTGGTAGTTTCTCCAGTATTATTAGGCTATTTGGTTTCACTT
ATAAAATTGCCTTCAAAGAGTTTTTTCATGGCTTTTATGAGTATTATAATATCAGAACTTCCACTCATGCAAATGTAACATTTGTGTGTT
AGTCTATTCTATAGCTCTGATCGGAGTCTTTATCTTAAATGGGAAAGATTATTTTCCCTGTCATGTAACTTTAACAGAAAACGATGCTAA
AATTAATTTTAAGACTGCTCTATACTATATGTATGTTATACTATATTTTTATATACTATACACAAAAATGTATAAGATGTAAAGCTTGTA
TTTTCAAATGTTATTGCAGTATCCAGAACAATTGCGACAATTGCTCTTGGAAGCTTCTGTGCTTTTCCACATGCTGTTCCCTGTCTCTGG
AATGGAATGTTCTCTTATTCTGGCTGGCAAACTCCTGAACATAATGTAAACAGTAAGCTCAAACATCACTTGCCCTTTCCTTACCATGCC
ATCCTGTTTCTTCTAAACCTTATAAATGCCTTTATTATAGTACATATTGAATCATAATGTAATTATTATTTATCTCTGACTCCTTTGATT
CTGGGACTGCCTTGAGGTCACGGTCTAGGTTTATTTCATCTTTCTGTCTGTTTAGTCAAATTTCCTACCCTGTGTAACTCTCAGAATCTT
ACTTTAAGTTAGGAAAGGCCAGTGGCTGGACAGATGCTACAGACCTCCATTGGTACAGATGCCCTTAGCTTCATGTTGGGAACAAGTGGT
TCTGTTTCTTTTTGTTCCTGTGGAGAAGCATCAGAGATACAGGCAGCTGGGGATTTCTACACCTGTAATAGCAGCAGCTCAGTGATGACA
CAGAGACATGTTTTTTTTTTTTTTTTTTTTTTGACAGAAGGCGAAGGGGAAGCAAGGTACTTCTTACATGGCAGAGGGAAAAGGAGAGTT
GGAGTGGGGGAGGGGGAGAGGGAGGAGCAGGAGCAGGGAGACGGGGAGGGAGAGGAAACTGCCCCGAAGACATGGTTTCTAAGATTTCTC
CCAATGAGACAAATCACTTGTCTGGGATGTCAGGGGGAGAAGATGCAGAATCTGAGCCCCTTGTATAAATAGACAAAAGGTGAAAGCATT
GGCAATAGATCAAAAAGGCATTCCACGTAGCAAAAAAATTATTTTTAAATCTGAAAATTTAAGTGAAATGCCAAGAGCAAAGAAGAGAGG
CCATCAGAAAAATTTCAAAGTGACTACTCAGGTCATTGTCCTGAAATAAATGATCATTTTATTCTGGTGACTCACCCCTAGCTAGAGTCA
GTACTTCATAGAAAAGACAGGAGCTGTGAGACACTTAACTGTAATCTTACCCCTTTACAGAAATGCCTCCTATAGTCAAGCTGGTCTGAT
GATCTCATGTCATCATGCACAAGGATTAGCAAGCCTTGAAATTTTATCCTGTCAGTGTAACTCCTCATCTAGTCTCAAGATTGCACTGGC
AGTTCTTTCGCAGCTGATCGTACAGGGAGGACCAATAAGGCTCATAGAACACCCTTGGAGAATGAATGCCTCTGCTGCTGGAGGTCACAG
CACCCAGAAAGTGACTAGAGGGAGCAGCTGATACCTGCTCTAGTGAGTTGTGCTATTCCTGTCACATCCTTGCAGAGGTCCAAGGGTTGC
TGGCAAGTGAAGGAATATCATGCCTGTAGTTTGAAGGATAGATCACTCAACTCTCAACTATTCTCTCTTCAGCTGTGAACCATACAACGT
TTTTGAAAAGCAAAGTCTGAACATTTGCATTCTATACAGAATATTACAGTTGGAAAATAGGTGTGGTGTCTAGAGAAATGCTTCTAAATG
CTGCAAGCCACCCTATGTGAAGCCTAGTCTAGAAATCTGCTTTGCTTCATTTATTAACTTCATTGTATCTTTGGAAGTAGAAGTTCTTAA
AAGTGTGTACAGAAGATGAGTCCTCAGCATGAGTTTCACTGCCTGTCTGTGTGAGAGGAAGTGACCTTGAGAGCAGCTGTAGCCCTAGGG
GGACACCCAGGTAGAGGTGCTCACAACATGCTCTGACACCCGGTATGGCTGCATTCCCAGCTTAAATGATCACATCTGCTCTTGGTTTTT
TCACTGTTTACAAAATCTGATTTCTTTAAAGGGAAGGGATGGAAAAGGTAGTCAATGCTCCTCTGAGCACCCAAAGCATCCCTCCACAAG
AACAAAGAGGAAAAATCATTACTTGCTCCCAACATTACACAAAGCGCATCTCTACCAATGGAACTCTGCAGTGTCGGTCTAGCCATCAGC
CTTTCCCACCTCACAGCATATTTTCTGTGGGGGTAATTTATTAGAGGGGAATTTGTTCCTTTTTACTCTTTTTACTCTTCAAATTAGTAA
TAGAACCCCTCATGTCATTCTAAGTGACTTAAGTTTTAAAGGGAACCAGAAAAGGAGAGAATATGCTATTGTGGCACTTCTAAAATGCAG
AGTGTCCTCAAAGATGGTTATAGTGGGTAATGGGTTTCATATAAATTGCATACTAGAGTATCTCCATTTCCTCTTTTAATTTTTTAATTG
GTTTTTTAAAGCTTGATATGCTGAGTTAGTTTTCATTTAAAAACAATGCTTGAGTAGGAAAGTTAAAGAAATAGAAATTGCCTGTTTTAA
TCTATTCATCCTGGCAGCAGGTATGTATTGTATCTGTCCAGAGTCCAAAACGTCAGCTCCCTCCACTTGCCTTCCCCATTTCACATAAAC
TATAAAAGTTAGAAATAATATATTATGTCATATCCTAACATCAGTTCAATGAATATAGTTTTTAAAATACCTACTATGTGCTAGATACTG
AGGACAGAATGATAAAATACAAAACAAAGCTAACTAAAAACACAGTTTAACTAGTCTTGTCTACGATTACCTGCCCTAACTATAACTTCA
TTTAAGGGGAGATACTGCTAAAGGGAGTTCCTTCTGTGACTGCACACTCTGATACCAATATGTGACTTCTCCCTGGCTACAACTGATTAA
ATGAGGGAAGAAAACTGATCCAAGGGGAGTTAGTCCAATAGCTTGTAAATGGTGGGTTCTTATCAGAGTTTTCTCACTAAGTTAGAACCA
AGAGGCTGAGGGTTGTGGGGGCTACTTAAGCAGAAAGTTTCATGGAGTATGGACTGTGCACCATTGTGAAGTTTCCTGTATTTCCACAGG
CTGTGGGGGCAGAGAGCATCAGAGTGCTGATCAGGAAAAAAGAATGAGGCAGATGCAGACAGAGAAGCAAAGAAGCATGAGAGGATGAGA
ATTAGGAAGTAGACTCTTTTTTCATTTTAATTAATTTGACTAGATTATATTTCTTGCAATCAAAAGGGCCCTGTGCAGAGAAGGTCCTTA
TCTTCGTTCATTTTTTGTTGCTATAGCTGAATACCCGAGACTGGGTAATTTATAAAGTAAAGAAATTTATTGCTTACCATTTTAGATGCT
GGGAAGTACAAGGTCAAGGGTTCTCATCTAGTGAGGGCCTTCTTGCTAGTGGGGTCTCTGTAGAGTCCTGAGGTAACACAGAGCATCACA
TGGTGTGGGGGTGGGATTCACCAGAGAGAGCCAAACTGACTTTTATAACAGACTCACTTTTGTGGTAACTAACCCACTCCCTTGATAGCC
CATCAATCCATTAATCTAGAAATAAATTCATTCACTCATGAGGGCAGAGCTCTTGTGAACCAATCACCTCCCAGAAGTCCCACCTCTCAA
CACTGCTGCACTGGGGACCAAATTTCCAACACATGAACTTTTCGGGGACACATTCAAGCCACAACAGTCCTCAATAAATATTTGTTGAAT
CAATAAATACACAGGACATAGTGTCCTAAATGCTTGGCATGTACATGAACCAGATTTGGTCCTTGATGTCACAGAGCTTATAATATAGTG
GAGGAGACAGACTTTACATGTTTGATCTTGACTGTAAAACTAGGGTCACTACTCATCATCTGATGAAGGAGGCAGACAGTAAAACAAATA
ACAACTATTCAACATGGTAACGTCAAAACTGAACAGAGGCTCAAGTGAAAGTTTCAATGATAGTAACCATGGCCAATGGATAGGTGAATG
CATTGGAAAATTAGTATGATGCCATTAGCAAAAACATAAATTGCACTTAACTAAAGTTCATCTGGCTGAATTCTTTCCTTCCTTCCTTCC
TTCCTTCCTTCCTTCCTTCCTTTCTTCCTCTATCTTTCTTTGGTTTTGAGATCTGTCTATAGTATTGTGCTAGTGCACATATTTTAATTT
GGTGCAAGGCATGCTTAACAGAGTAAGTGAAAAGCTATAAAGGTTAGAGCGTGTATTAGTTTCAATGACGAAGGAGTAATAAATCATTTT
GTCAGGTTGCACTCTAACATTCAAATTCCATTTTTATTATGGCTAACTAGCCAAGCATTTATAATTCTGAACTCCACAGAATGGGGAAAA
AGTGTATTAACTTATAATAGATCATTGAGCTTGTTAAATGTACCTTTCCTAATTCGTACTTTTTCCCTTTTGCTGAGAAAAATGCCTTTC
TGAATATATAATTTTTACAGGTCATTGGAAAGGAGTACTGCACAACAGGATTATTATAATGTGCTAAAAGATGGCACTTGTATCTCATTG
CCACATAGAGAGCTCATGTGAAAACTCTTTGATCTTTGCTGGCTTTTTAGCTGAAAAGATTTGGAAATCTTTTTGGCACTGGCAAAAACA
GAACTGTGGTAACTGTTTATAGTTGGTTGATTTTTAGCAATGGGTAAGAATGTCTTTGTTGAATCCAGTTTATTAATACGTAATGGGCAA
CATGTTGCCTTAAAGGCAAAAGCATATTTTTCTCATATCAAAAAGCTCCCTAAATTTTTGCCAATATCCAAAGATATTTTAAGATGGTCT
TCATTTTCTTTTTCAGTCTGTCCTATGCTGAGAAAAAAAAATTACAACCTCTGAACTTCAGCAGTTTTTTAGGTAAAAGGAATTTTTCAT
GATGTGATTGATATAACTACTCAGCTGGACATAAGCTTTCAAGGGCTAAGTCCATTTGCCGATTTGTTTGCATTTCCTACTCCAACTAAA
ATAATTCAAAGACATGAAGTGTCTATTGTGGAAAATGCATGAATAATGCAAAAGTCATTCTCAAGTGACCTATCAGAAAGGCCTACCAGA
AATGCAGTTACTGCTTTCTAATAAAATCATGAAAAACTTTTTTAAAAAGTATAACCATCTTGAATTTGTTGAGGCAATTATAAGAGAGTA
TATAAGGGCAAGTTTCTCCAAAAGCGGTTCTGTTTGTTTTGTTTTTTTTTAATAATTCCCATCAACAGCAATCTGTTTTCTCATTGTAGT
AGTATAGGTGTTAACATAAAAGCTCAAACAGCTTGGGAGAAAAAAAAGGATATAGCAAACCTAACTGATAACTTTGCTAGTTTCAAATCA
TTATGATGGCCATTCATAGATGACTGAAATATTTAACATTTGAAGTAACAGAATTTTGACTTATGTTTTCATGGTTTAAGAGTTTATATT
TCAAATTTTTTCACATAGTCTGTTTATAACAGTATATAAAACATGTATTAACTATATAAGTTAATAAAGTCATGAACTGATGGCCATTTT
TTGGTCTTATTCATATAGTATAGGCTGTTTTGTGAATTAATCCCCTTCCCATGTGTTTGAATTCCCCACTATGTAAGGCTTAGTGGTGTT
AGCATGATCCAAGGGTGGGATTTTCAGCCCTCTGACATCAAAAGGTGAAGCAGAGGACACAAAAACCCTCACTGCATCTTCCCTAGACTG
GCCAGAACCACTCCATGGTCAGTGGACTCCCTTAATAGGAAGGAATGCTGGTCAGTTGTCAAAGAGCAAAAAGGGAGGGGTAGTCATGAG
GTTGGTTGTAATCAGAGGTGCAACAGGTCTTTCCAAAGGGCTGGTTTCTGTTTAACTCTTAGGAAAGAACACCTAATGGTAGTTAGTGAG
GAGGGGGTATAATGAGATGTGCCCAACCTCCCATCCCATCATGGCCGGGAACTCAGTTTTTAAGGTTTCCCTGAAGTCCTCTTGGCCAGG
AGAGGGTCTGTTTAGTCAAATGTGGGGGGGGAGCTTAGGATTTTATTTTTATTTCCAAGTTATAATGTTGGACCATCAGAGCATGAATAC
AGGTTGAGTCTTTAATCTGTAAATACTTAGCAATCACCTTCTTCTCAGTAACTTTCTCTGAAGGTTGCTGATAAGCCAGTGTTCTTGATG
TTTGCCCACTTACCCCTCAAGATGAGGAGTGAAGAAATTAGATTGCATTGCCAGGAATTTCCTAGAAGAAAAGGTTTTGAGGATGCATGA
CCTATGAGGGTGCTGAGGACCCCTTTCCACCAAACATGGACTAACTTCTGGGTTGCTCTTTCACATCTATTGTCTTGTTCGACGTACACA
GAAGTGCAGAGCACAGCCGCAGCCCTCACAGCATCATCTATCTAGGTTCTGAATCCTGAATGGTCTGAGGCTCTCCTAGAGCTGAGATTC
TCCTTTGCAGTTCCAGTGGAGAAAAGCACTTTCTTTGCCGAGGCAGTGTAACTTCCTTGTTCCCAAGGCTCCAACTGTTGTGGGCACATA
TGAGTATACATTAACATCCATGCCCAATTTATAATCACTGTTTTCCCCACATGTGTACTCAGACCTCCAAGCTTCTGAAAAATATTTCCT
CTCATCTCTCAAAGAATTTTAGTACATTTGCCTGGATGCAGAAACACAAATCATGCTGGCTTTCCTACTCAATCAAAAACTGTAGCTTGA
GTGGGGTGGCAAGGGTTGTCCCACAGCAGGTTTACTTGGACTCCAAAATGATGTAGAGTTTAGTGAGGCAGCTCCTTGAGTTTGAACTTA
GAGCCCTTTTGGCTACCAGGGCACCTGCAGTCCTCCAAGATAGTAGAGTCTGAACCAGTTAAAGAGCCAGCTACCACAGCTTTGCAGAAA
CCACTGTTTCTCCTTAACTCTAGGGCTCAGCGGTTGAACTCCTTAGAATAAAACTTTCTGTCTGCGACCCTATCATTTTCTTTTGTATTT
TGGCCTTCACTGAATTCCCACCCCATCCATTCCAACAGACTTGGTTATACGTGTCTGAACACCAAATGTGACAGAATTTGTGGCCTTGGA
TCAGGAGGCTATTTGGCCCTTATCTAAATCGGTGGCTGAGAATGGATTCCAGGGAAGTGTTTTTCTTAGGAGTACCCAAGAAGCTCAGCT
CAGGTTGGGCTCCCTGTTCTATTCTGAGAACTACAAATCAAATTCAAAATGGAGCACAGAAAATTCTCATTTCTAGTTGACTTATTTGTA
TTTTGGTGTCCTGTGCCCTAAATATCTTCTCAAGGAAACCTTGGCTTTAAAAGTGATTGTTTACTTAAACAAATAAAACAACAACATACA
AGAAAAGGCAGTAAATCCGAGCGTCCATCAACAAATGAATCAAAAAACAAATGAGATATATATGTACGGTGGCATATTATTCAGCCTTAA
AAAGGAAAGAAATTTTGACACATGAGACAATATGAATGAACCTTGAAGACATTAATTATGCTGAGTGAAATAAGCCAGCCACAAAAGAAC
AGATATTGTATAATTCCTCTTATATTAGGTACCTAAAATAGTCAAATTGATAGAAAGTAGAATGTTGGTTGGCAGGGGCTGGCAAGGAGA
GGGAAATGAAGAGAAGGTGTATGAAGGGAACAGAGTTTCAGTTGGGGAAGACGAAAAAGTTCTGACAGTGGATGATGGTGGTTGTTGCAC
AGCAACATGAATGTACTTAATGCCACAGAACTGTATGCTTAAAAATGGTTGAAACGGTAAATTTGATATAATGTGTATTGAACCTATTTC

>In-frame_ENST00000295119_ENST00000361354_TCGA-43-8115-01A_NUP35_chr2_183989225_+_NCKAP1_chr2_183853914_length(amino acids)=887AA_start in transcript=76_stop in transcript=2739
MVLSVVADAMAAFAVEPQGPALVGFILCHGILNTDATALNLWKLALQSSSCLSLFRDEVFHIHKAAEDLFVNIRGYNKRINDIRECKEAA
VSHAGSMHRERRKFLRSALKELATVLSDQPGLLGPKALFVFMALSFARDEIIWLLRHADNMPKKSADDFIDKHIAELIFYMEELRAHVRK
YGPVMQRYYVQYLSGFDAVVLNELVQNLSVCPEDESIIMSSFVNTMTSLSVKQVEDGEVFDFRGMRLDWFRLQAYTSVSKASLGLADHRE
LGKMMNTIIFHTKMVDSLVEMLVETSDLSIFCFYSRAFEKMFQQCLELPSQSRYSIAFPLLCTHFMSCTHELCPEERHHIGDRSLSLCNM
FLDEMAKQARNLITDICTEQCTLSDQLLPKHCAKTISQAVNKKSKKQTGKKGEPEREKPGVESMRKNRLVVTNLDKLHTALSELCFSINY
VPNMVVWEHTFTPREYLTSHLEIRFTKSIVGMTMYNQATQEIAKPSELLTSVRAYMTVLQSIENYVQIDITRVFNNVLLQQTQHLDSHGE
PTITSLYTNWYLETLLRQVSNGHIAYFPAMKAFVNLPTENELTFNAEEYSDISEMRSLSELLGPYGMKFLSESLMWHISSQVAELKKLVV
ENVDVLTQMRTSFDKPDQMAALFKRLSSVDSVLKRMTIIGVILSFRSLAQEALRDVLSYHIPFLVSSIEDFKDHIPRETDMKVAMNVYEL
SSAAGLPCEIDPALVVALSSQKSENISPEEEYKIACLLMVFVAVSLPTLASNVMSQYSPAIEGHCNNIHCLAKAINQIAAALFTIHKGSI

--------------------------------------------------------------
>In-frame_ENST00000295119_ENST00000360982_TCGA-43-8115-01A_NUP35_chr2_183989225_+_NCKAP1_chr2_183853914_length(transcript)=3565nt_BP=143nt
ACGCAACCCCATAAGGTAGCACGCCGTTACCCGTGGGGAGCGTTTCCGCCATTTTTGAAAATTAATTGGGAAGGTACTGGTTTTAAGTGT
AGTTGCCGACGCAATGGCAGCCTTTGCAGTGGAACCTCAGGGGCCCGCGTTAGTTGGCTTTATTTTGTGCCATGGGATCCTAAATACTGA
CGCTACAGCACTGAACCTTTGGAAACTAGCTCTTCAAAGTAGCTCTTGCCTCTCTCTCTTTCGGGATGAAGTTTTCCACATTCACAAAGC
TGCAGAAGACTTATTTGTAAACATACGAGGCTATAATAAACGTATTAATGACATAAGAGAATGCAAGGAGGCAGCCGTGTCACATGCTGG
TTCAATGCACAGAGAAAGACGCAAGTTTTTAAGATCTGCACTGAAGGAATTGGCTACTGTCCTCTCTGATCAACCTGGATTGCTAGGTCC
CAAGGCACTTTTTGTTTTTATGGCATTATCCTTTGCCCGTGATGAAATCATCTGGCTACTTCGTCATGCAGATAACATGCCAAAGAAGAG
TGCAGACGACTTTATAGATAAGCACATTGCTGAATTAATATTTTACATGGAAGAACTTAGAGCACATGTGAGGAAATACGGACCTGTAAT
GCAGAGGTATTACGTGCAGTACCTTTCTGGCTTTGATGCTGTTGTCCTCAATGAACTCGTGCAGAATCTTTCTGTTTGCCCTGAAGATGA
ATCAATCATCATGTCCTCTTTTGTTAACACTATGACTTCCCTAAGTGTAAAACAAGTTGAAGATGGGGAAGTATTTGATTTCAGAGGAAT
GAGATTAGATTGGTTTAGGTTACAGGCATATACTAGTGTCTCAAAGGCTTCACTTGGCCTTGCAGATCACAGAGAACTTGGAAAGATGAT
GAATACAATAATTTTTCATACAAAAATGGTAGATTCCTTGGTGGAAATGTTGGTGGAAACATCAGATCTCTCCATATTTTGTTTTTATAG
TCGTGCTTTTGAGAAGATGTTTCAACAGTGTTTGGAGTTACCCTCTCAATCAAGATACTCAATTGCATTTCCACTACTTTGCACTCATTT
TATGAGTTGCACGCATGAACTATGTCCAGAAGAGCGACATCATATTGGAGATCGCAGTCTTTCCTTATGTAATATGTTCCTAGATGAAAT
GGCCAAACAAGCTCGAAATCTCATCACTGATATTTGCACAGAACAGTGTACCCTTAGTGACCAGTTGCTACCCAAGCATTGTGCCAAAAC
TATCAGTCAAGCAGTGAATAAGAAATCAAAAAAGCAGACTGGTAAGAAAGGGGAACCTGAAAGGGAGAAACCAGGTGTTGAGAGCATGAG
GAAAAACAGGCTGGTTGTGACCAACCTTGATAAATTGCACACTGCACTTTCTGAGTTATGCTTCTCTATAAATTATGTACCAAACATGGT
GGTATGGGAACATACCTTTACCCCACGAGAATATTTGACTTCTCATCTGGAAATACGCTTTACCAAGTCAATTGTTGGGATGACTATGTA
TAATCAAGCCACACAGGAAATTGCAAAACCTTCAGAACTTCTAACAAGTGTAAGAGCATACATGACCGTACTCCAGTCAATAGAAAACTA
TGTGCAGATTGATATTACAAGAGTATTTAATAATGTGCTTCTTCAACAAACACAACATTTAGACAGTCATGGAGAGCCAACCATTACAAG
TCTATACACAAATTGGTATTTGGAAACTTTGTTACGACAAGTCAGCAATGGCCATATAGCATATTTTCCTGCAATGAAAGCGTTTGTGAA
CTTACCTACAGAAAATGAATTAACATTCAATGCAGAGGAATATTCTGACATATCAGAAATGAGGTCATTATCAGAACTACTAGGCCCATA
TGGTATGAAGTTTCTAAGTGAAAGCCTTATGTGGCATATTTCATCACAAGTTGCTGAACTTAAGAAACTTGTGGTGGAGAATGTTGATGT
GTTAACACAAATGAGGACCAGCTTTGACAAACCAGACCAGATGGCTGCACTGTTTAAAAGATTATCATCTGTTGACAGTGTCTTGAAGAG
GATGACAATAATTGGTGTAATTTTATCCTTCCGATCATTGGCACAAGAAGCACTTAGAGATGTCTTATCCTACCACATTCCTTTTCTTGT
AAGTTCAATTGAAGATTTTAAGGATCACATTCCAAGGGAAACTGATATGAAGGTTGCAATGAATGTGTATGAGTTATCATCAGCTGCCGG
ATTACCTTGTGAGATTGATCCTGCATTGGTCGTAGCTCTTTCTTCACAAAAATCGGAAAACATTAGTCCAGAAGAAGAGTATAAAATTGC
CTGCCTTCTCATGGTGTTTGTGGCAGTTTCTTTGCCAACACTGGCCAGTAATGTGATGTCTCAGTACAGCCCTGCTATAGAAGGGCATTG
CAACAACATACATTGCTTGGCCAAAGCCATCAACCAGATTGCTGCAGCTTTGTTTACAATTCACAAAGGAAGCATTGAAGACCGTCTTAA
AGAATTTCTGGCGCTTGCATCCTCCAGTCTACTGAAAATTGGCCAGGAGACAGATAAAACTACAACAAGAAATAGAGAATCTGTTTATTT
ACTGCTAGATATGATTGTACAAGAATCTCCATTCCTTACAATGGATCTTTTGGAATCTTGTTTTCCTTATGTCTTGCTGAGAAATGCATA
CCATGCTGTCTACAAACAAAGTGTTACATCTTCTGCATAAAATTACCTACTTAATCAAGATAAGCACGCATTTTTGTTGCCTTGGTTTTA
CCTGTAGACTGTGGAACTATTTTACCTTAAGACCTGAAAAAGTTTTGTGGATTATAAATTTCTTTCATACGGTTGTATTTTCTGATCATT
GGTTTCTTAATATGGTTGTACTACAGTATACTTGGTTGATTTAGGTTGCACATTCACTGAATTCACTGAGATTATTCCTATAATTTTAAA
GTATCATTTATTTGAAAAACATACATTATCAACATGTTTTTGATATTTGATAATGAAAAAAATCTTTGCTTGTTTATTTCTGAAAAAGAA
CTGTATTTAGTGATTATTTTAGATAGTGATATTATAGTATTCATCTGTGTGTAAATTATTTCATATAGGGAAGAGTTCTGATCTGTACCT
ATGGTTCTTATTGAAAACAACATTGGATGTGCATTTCTGTGATGTTATGAATACATTTCTACTTTATTTTGAAACATTTGCCAAACTAAA
TACTGTAACACTGTATAACATTTAAAAATGTTAAAGAACTGCTTAGTATTAGAAGCAGATCATTTCCCAAAATTCTAAGAGCAGCAGCAT
ATGTTGTTGCTTGTATAAAGCCTAGCGATAATTTTTAGACTAACTTCCATGGTGCCCTGTTGGCATTAGCACTACCATTGTACCCTGCTG
TATAATAAACAATCTTAGACATTTATCAACTGTTGATACAAATGTTAGTCCCTAACCACTTTTTATATATGTTTTAAATTTTTGAAATTC

>In-frame_ENST00000295119_ENST00000360982_TCGA-43-8115-01A_NUP35_chr2_183989225_+_NCKAP1_chr2_183853914_length(amino acids)=887AA_start in transcript=76_stop in transcript=2739
MVLSVVADAMAAFAVEPQGPALVGFILCHGILNTDATALNLWKLALQSSSCLSLFRDEVFHIHKAAEDLFVNIRGYNKRINDIRECKEAA
VSHAGSMHRERRKFLRSALKELATVLSDQPGLLGPKALFVFMALSFARDEIIWLLRHADNMPKKSADDFIDKHIAELIFYMEELRAHVRK
YGPVMQRYYVQYLSGFDAVVLNELVQNLSVCPEDESIIMSSFVNTMTSLSVKQVEDGEVFDFRGMRLDWFRLQAYTSVSKASLGLADHRE
LGKMMNTIIFHTKMVDSLVEMLVETSDLSIFCFYSRAFEKMFQQCLELPSQSRYSIAFPLLCTHFMSCTHELCPEERHHIGDRSLSLCNM
FLDEMAKQARNLITDICTEQCTLSDQLLPKHCAKTISQAVNKKSKKQTGKKGEPEREKPGVESMRKNRLVVTNLDKLHTALSELCFSINY
VPNMVVWEHTFTPREYLTSHLEIRFTKSIVGMTMYNQATQEIAKPSELLTSVRAYMTVLQSIENYVQIDITRVFNNVLLQQTQHLDSHGE
PTITSLYTNWYLETLLRQVSNGHIAYFPAMKAFVNLPTENELTFNAEEYSDISEMRSLSELLGPYGMKFLSESLMWHISSQVAELKKLVV
ENVDVLTQMRTSFDKPDQMAALFKRLSSVDSVLKRMTIIGVILSFRSLAQEALRDVLSYHIPFLVSSIEDFKDHIPRETDMKVAMNVYEL
SSAAGLPCEIDPALVVALSSQKSENISPEEEYKIACLLMVFVAVSLPTLASNVMSQYSPAIEGHCNNIHCLAKAINQIAAALFTIHKGSI

--------------------------------------------------------------

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Fusion Gene PPI Analysis for NUP35-NCKAP1


check button Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in

ChiPPI page.


check button Protein-protein interactors with each fusion partner protein in wild-type (BIOGRID-3.4.160)
HgeneHgene's interactorsTgeneTgene's interactors


check button - Retained PPIs in in-frame fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenStill interaction with


check button - Lost PPIs in in-frame fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenInteraction lost with


check button - Retained PPIs, but lost function due to frame-shift fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenInteraction lost with


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Related Drugs for NUP35-NCKAP1


check button Drugs targeting genes involved in this fusion gene.
(DrugBank Version 5.1.8 2021-05-08)
PartnerGeneUniProtAccDrugBank IDDrug nameDrug activityDrug typeDrug status

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Related Diseases for NUP35-NCKAP1


check button Diseases associated with fusion partners.
(DisGeNet 4.0)
PartnerGeneDisease IDDisease name# pubmedsSource
TgeneNCKAP1C1535926Neurodevelopmental Disorders1CTD_human