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Center for Computational Systems Medicine
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Fusion Gene Summary

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Fusion Gene ORF analysis

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Fusion Genomic Features

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Fusion Protein Features

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Fusion Gene Sequence

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Fusion Gene PPI analysis

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Related Drugs

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Related Diseases

Fusion gene:PIK3CB-SEMA5B (FusionGDB2 ID:64972)

Fusion Gene Summary for PIK3CB-SEMA5B

check button Fusion gene summary
Fusion gene informationFusion gene name: PIK3CB-SEMA5B
Fusion gene ID: 64972
HgeneTgene
Gene symbol

PIK3CB

SEMA5B

Gene ID

5291

54437

Gene namephosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit betasemaphorin 5B
SynonymsP110BETA|PI3K|PI3KBETA|PIK3C1SEMAG|SemG
Cytomap

3q22.3

3q21.1

Type of geneprotein-codingprotein-coding
Descriptionphosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit beta isoformPI3-kinase p110 subunit betaPI3-kinase subunit betaPI3K-betaPtdIns-3-kinase p110phosphatidylinositol 4,5-bisphosphate 3-kinase 110 kDa catalytic subunit betaphosphoinositidsemaphorin-5Bsema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5B
Modification date2020031320200313
UniProtAcc..
Ensembl transtripts involved in fusion geneENST00000477593, ENST00000544716, 
ENST00000289153, 		ENST00000357599, 
ENST00000195173, ENST00000451055, 
ENST00000465147, 
Fusion gene scores* DoF score15 X 13 X 11=21454 X 4 X 3=48
# samples 176
** MAII scorelog2(17/2145*10)=-3.65737099624921
possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs).
DoF>8 and MAII<0		log2(6/48*10)=0.321928094887362
effective Gene in Pan-Cancer Fusion Genes (eGinPCFGs).
DoF>8 and MAII>0
Context

PubMed: PIK3CB [Title/Abstract] AND SEMA5B [Title/Abstract] AND fusion [Title/Abstract]

Most frequent breakpointPIK3CB(138452203)-SEMA5B(122667556), # samples:3
Anticipated loss of major functional domain due to fusion event.PIK3CB-SEMA5B seems lost the major protein functional domain in Hgene partner, which is a CGC due to the frame-shifted ORF.
PIK3CB-SEMA5B seems lost the major protein functional domain in Hgene partner, which is a cell metabolism gene due to the frame-shifted ORF.
PIK3CB-SEMA5B seems lost the major protein functional domain in Hgene partner, which is a IUPHAR drug target due to the frame-shifted ORF.
* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

check button Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez
PartnerGeneGO IDGO termPubMed ID
HgenePIK3CB

GO:0016310

phosphorylation

25327288


check buttonFusion gene breakpoints across PIK3CB (5'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

check buttonFusion gene breakpoints across SEMA5B (3'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

check button Fusion gene information from two resources (ChiTars 5.0 and ChimerDB 4.0)
* All genome coordinats were lifted-over on hg19.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.
SourceDiseaseSampleHgeneHchrHbpHstrandTgeneTchrTbpTstrand
ChimerDB4KIRPTCGA-G7-6793-01APIK3CBchr3

138452203

-SEMA5Bchr3

122667556

-
ChimerDB4KIRPTCGA-G7-6793PIK3CBchr3

138452202

-SEMA5Bchr3

122667556

-
ChimerDB4KIRPTCGA-G7-6793PIK3CBchr3

138452203

-SEMA5Bchr3

122667556

-
ChimerDB4KIRPTCGA-G7-6793-01APIK3CBchr3

138452203

-SEMA5Bchr3

122667556

-


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Fusion Gene ORF analysis for PIK3CB-SEMA5B

check button Open reading frame (ORF) analsis of fusion genes based on Ensembl gene isoform structure.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.
ORFHenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrand
In-frameENST00000477593ENST00000357599PIK3CBchr3

138452203

-SEMA5Bchr3

122667556

-
In-frameENST00000477593ENST00000195173PIK3CBchr3

138452203

-SEMA5Bchr3

122667556

-
In-frameENST00000477593ENST00000451055PIK3CBchr3

138452203

-SEMA5Bchr3

122667556

-
5CDS-5UTRENST00000477593ENST00000465147PIK3CBchr3

138452203

-SEMA5Bchr3

122667556

-
intron-3CDSENST00000544716ENST00000357599PIK3CBchr3

138452203

-SEMA5Bchr3

122667556

-
intron-3CDSENST00000544716ENST00000195173PIK3CBchr3

138452203

-SEMA5Bchr3

122667556

-
intron-3CDSENST00000544716ENST00000451055PIK3CBchr3

138452203

-SEMA5Bchr3

122667556

-
intron-5UTRENST00000544716ENST00000465147PIK3CBchr3

138452203

-SEMA5Bchr3

122667556

-
Frame-shiftENST00000289153ENST00000357599PIK3CBchr3

138452203

-SEMA5Bchr3

122667556

-
Frame-shiftENST00000289153ENST00000195173PIK3CBchr3

138452203

-SEMA5Bchr3

122667556

-
Frame-shiftENST00000289153ENST00000451055PIK3CBchr3

138452203

-SEMA5Bchr3

122667556

-
5CDS-5UTRENST00000289153ENST00000465147PIK3CBchr3

138452203

-SEMA5Bchr3

122667556

-
In-frameENST00000477593ENST00000357599PIK3CBchr3

138452202

-SEMA5Bchr3

122667556

-
In-frameENST00000477593ENST00000195173PIK3CBchr3

138452202

-SEMA5Bchr3

122667556

-
In-frameENST00000477593ENST00000451055PIK3CBchr3

138452202

-SEMA5Bchr3

122667556

-
5CDS-5UTRENST00000477593ENST00000465147PIK3CBchr3

138452202

-SEMA5Bchr3

122667556

-
intron-3CDSENST00000544716ENST00000357599PIK3CBchr3

138452202

-SEMA5Bchr3

122667556

-
intron-3CDSENST00000544716ENST00000195173PIK3CBchr3

138452202

-SEMA5Bchr3

122667556

-
intron-3CDSENST00000544716ENST00000451055PIK3CBchr3

138452202

-SEMA5Bchr3

122667556

-
intron-5UTRENST00000544716ENST00000465147PIK3CBchr3

138452202

-SEMA5Bchr3

122667556

-
Frame-shiftENST00000289153ENST00000357599PIK3CBchr3

138452202

-SEMA5Bchr3

122667556

-
Frame-shiftENST00000289153ENST00000195173PIK3CBchr3

138452202

-SEMA5Bchr3

122667556

-
Frame-shiftENST00000289153ENST00000451055PIK3CBchr3

138452202

-SEMA5Bchr3

122667556

-
5CDS-5UTRENST00000289153ENST00000465147PIK3CBchr3

138452202

-SEMA5Bchr3

122667556

-

check buttonORFfinder result based on the fusion transcript sequence of in-frame fusion genes.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandSeq length
(transcript)		BP loci
(transcript)		Predicted start
(transcript)		Predicted stop
(transcript)		Seq length
(amino acids)
ENST00000477593PIK3CBchr3138452203-ENST00000357599SEMA5Bchr3122667556-54051124113844551105
ENST00000477593PIK3CBchr3138452203-ENST00000195173SEMA5Bchr3122667556-55281124113842781046
ENST00000477593PIK3CBchr3138452203-ENST00000451055SEMA5Bchr3122667556-49351124113844551105
ENST00000477593PIK3CBchr3138452202-ENST00000357599SEMA5Bchr3122667556-54051124113844551105
ENST00000477593PIK3CBchr3138452202-ENST00000195173SEMA5Bchr3122667556-55281124113842781046
ENST00000477593PIK3CBchr3138452202-ENST00000451055SEMA5Bchr3122667556-49351124113844551105

check buttonDeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandNo-coding scoreCoding score
ENST00000477593ENST00000357599PIK3CBchr3138452203-SEMA5Bchr3122667556-0.0154933380.98450667
ENST00000477593ENST00000195173PIK3CBchr3138452203-SEMA5Bchr3122667556-0.0171793570.98282063
ENST00000477593ENST00000451055PIK3CBchr3138452203-SEMA5Bchr3122667556-0.016528680.98347133
ENST00000477593ENST00000357599PIK3CBchr3138452202-SEMA5Bchr3122667556-0.0154933380.98450667
ENST00000477593ENST00000195173PIK3CBchr3138452202-SEMA5Bchr3122667556-0.0171793570.98282063
ENST00000477593ENST00000451055PIK3CBchr3138452202-SEMA5Bchr3122667556-0.016528680.98347133

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Fusion Genomic Features for PIK3CB-SEMA5B


check buttonFusionAI prediction of the potential fusion gene breakpoint based on the pre-mature RNA sequence context (+/- 5kb of individual partner genes, total 20kb length sequence). FusionAI is a fusion gene breakpoint classifier based on convolutional neural network by comparing the fusion positive and negative sequence context of ~ 20K fusion gene data. From here, we can have the relative potentency of the 20K genomic sequence how individual sequnce will be likely used as the gene fusion breakpoints.
HgeneHchrHbpHstrandTgeneTchrTbpTstrand1-pp (fusion gene breakpoint)

check buttonDistribution of 44 human genomic features loci across 20kb length fusion breakpoint regions. We integrated a total of 44 different types of human genomic feature loci information across five big categories including virus integration sites, repeats, structural variants, chromatin states, and gene expression regulation. More details are in help page.

check buttonDistribution of 44 human genomic features loci across 20kb length fusion breakpoint regions that are ovelapped with the top 1% feature importance score regions. More details are in help page.

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Fusion Protein Features for PIK3CB-SEMA5B


check button Go to

FGviewer for the breakpoints of chr3:138452203-chr3:122667556

.
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
FGviewer

check buttonMain function of each fusion partner protein. (from UniProt)
HgeneTgene
..
FUNCTION: Might normally function as a transcriptional repressor. EWS-fusion-proteins (EFPS) may play a role in the tumorigenic process. They may disturb gene expression by mimicking, or interfering with the normal function of CTD-POLII within the transcription initiation complex. They may also contribute to an aberrant activation of the fusion protein target genes.FUNCTION: Might normally function as a transcriptional repressor. EWS-fusion-proteins (EFPS) may play a role in the tumorigenic process. They may disturb gene expression by mimicking, or interfering with the normal function of CTD-POLII within the transcription initiation complex. They may also contribute to an aberrant activation of the fusion protein target genes.

check buttonRetention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at

download page


* Minus value of BPloci means that the break pointn is located before the CDS.
- In-frame and retained protein feature among the 13 regional features.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note
HgenePIK3CBchr3:138452202chr3:122667556ENST00000289153-622194_285350.01071.0DomainPI3K-RBD
HgenePIK3CBchr3:138452202chr3:122667556ENST00000289153-62226_115350.01071.0DomainPI3K-ABD
HgenePIK3CBchr3:138452202chr3:122667556ENST00000477593-723194_285350.01071.0DomainPI3K-RBD
HgenePIK3CBchr3:138452202chr3:122667556ENST00000477593-72326_115350.01071.0DomainPI3K-ABD
HgenePIK3CBchr3:138452203chr3:122667556ENST00000289153-622194_285350.01071.0DomainPI3K-RBD
HgenePIK3CBchr3:138452203chr3:122667556ENST00000289153-62226_115350.01071.0DomainPI3K-ABD
HgenePIK3CBchr3:138452203chr3:122667556ENST00000477593-723194_285350.01071.0DomainPI3K-RBD
HgenePIK3CBchr3:138452203chr3:122667556ENST00000477593-72326_115350.01071.0DomainPI3K-ABD
TgeneSEMA5Bchr3:138452202chr3:122667556ENST00000357599123103_55341.3333333333333361152.0DomainSema
TgeneSEMA5Bchr3:138452202chr3:122667556ENST00000357599123664_72041.3333333333333361152.0DomainTSP type-1 1
TgeneSEMA5Bchr3:138452202chr3:122667556ENST00000357599123722_77141.3333333333333361152.0DomainTSP type-1 2
TgeneSEMA5Bchr3:138452202chr3:122667556ENST00000357599123853_90841.3333333333333361152.0DomainTSP type-1 3
TgeneSEMA5Bchr3:138452202chr3:122667556ENST00000357599123910_96541.3333333333333361152.0DomainTSP type-1 4
TgeneSEMA5Bchr3:138452202chr3:122667556ENST00000357599123966_101041.3333333333333361152.0DomainTSP type-1 5
TgeneSEMA5Bchr3:138452202chr3:122667556ENST00000451055123103_55395.333333333333331206.0DomainSema
TgeneSEMA5Bchr3:138452202chr3:122667556ENST00000451055123664_72095.333333333333331206.0DomainTSP type-1 1
TgeneSEMA5Bchr3:138452202chr3:122667556ENST00000451055123722_77195.333333333333331206.0DomainTSP type-1 2
TgeneSEMA5Bchr3:138452202chr3:122667556ENST00000451055123853_90895.333333333333331206.0DomainTSP type-1 3
TgeneSEMA5Bchr3:138452202chr3:122667556ENST00000451055123910_96595.333333333333331206.0DomainTSP type-1 4
TgeneSEMA5Bchr3:138452202chr3:122667556ENST00000451055123966_101095.333333333333331206.0DomainTSP type-1 5
TgeneSEMA5Bchr3:138452203chr3:122667556ENST00000357599123103_55341.3333333333333361152.0DomainSema
TgeneSEMA5Bchr3:138452203chr3:122667556ENST00000357599123664_72041.3333333333333361152.0DomainTSP type-1 1
TgeneSEMA5Bchr3:138452203chr3:122667556ENST00000357599123722_77141.3333333333333361152.0DomainTSP type-1 2
TgeneSEMA5Bchr3:138452203chr3:122667556ENST00000357599123853_90841.3333333333333361152.0DomainTSP type-1 3
TgeneSEMA5Bchr3:138452203chr3:122667556ENST00000357599123910_96541.3333333333333361152.0DomainTSP type-1 4
TgeneSEMA5Bchr3:138452203chr3:122667556ENST00000357599123966_101041.3333333333333361152.0DomainTSP type-1 5
TgeneSEMA5Bchr3:138452203chr3:122667556ENST00000451055123103_55395.333333333333331206.0DomainSema
TgeneSEMA5Bchr3:138452203chr3:122667556ENST00000451055123664_72095.333333333333331206.0DomainTSP type-1 1
TgeneSEMA5Bchr3:138452203chr3:122667556ENST00000451055123722_77195.333333333333331206.0DomainTSP type-1 2
TgeneSEMA5Bchr3:138452203chr3:122667556ENST00000451055123853_90895.333333333333331206.0DomainTSP type-1 3
TgeneSEMA5Bchr3:138452203chr3:122667556ENST00000451055123910_96595.333333333333331206.0DomainTSP type-1 4
TgeneSEMA5Bchr3:138452203chr3:122667556ENST00000451055123966_101095.333333333333331206.0DomainTSP type-1 5
TgeneSEMA5Bchr3:138452202chr3:122667556ENST000003575991231058_115141.3333333333333361152.0Topological domainCytoplasmic
TgeneSEMA5Bchr3:138452202chr3:122667556ENST000004510551231058_115195.333333333333331206.0Topological domainCytoplasmic
TgeneSEMA5Bchr3:138452203chr3:122667556ENST000003575991231058_115141.3333333333333361152.0Topological domainCytoplasmic
TgeneSEMA5Bchr3:138452203chr3:122667556ENST000004510551231058_115195.333333333333331206.0Topological domainCytoplasmic
TgeneSEMA5Bchr3:138452202chr3:122667556ENST000003575991231037_105741.3333333333333361152.0TransmembraneHelical%3B Signal-anchor for type III membrane protein
TgeneSEMA5Bchr3:138452202chr3:122667556ENST000004510551231037_105795.333333333333331206.0TransmembraneHelical%3B Signal-anchor for type III membrane protein
TgeneSEMA5Bchr3:138452203chr3:122667556ENST000003575991231037_105741.3333333333333361152.0TransmembraneHelical%3B Signal-anchor for type III membrane protein
TgeneSEMA5Bchr3:138452203chr3:122667556ENST000004510551231037_105795.333333333333331206.0TransmembraneHelical%3B Signal-anchor for type III membrane protein

- In-frame and not-retained protein feature among the 13 regional features.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note
HgenePIK3CBchr3:138452202chr3:122667556ENST00000289153-622327_496350.01071.0DomainC2 PI3K-type
HgenePIK3CBchr3:138452202chr3:122667556ENST00000289153-622524_701350.01071.0DomainPIK helical
HgenePIK3CBchr3:138452202chr3:122667556ENST00000289153-622800_1067350.01071.0DomainPI3K/PI4K
HgenePIK3CBchr3:138452202chr3:122667556ENST00000477593-723327_496350.01071.0DomainC2 PI3K-type
HgenePIK3CBchr3:138452202chr3:122667556ENST00000477593-723524_701350.01071.0DomainPIK helical
HgenePIK3CBchr3:138452202chr3:122667556ENST00000477593-723800_1067350.01071.0DomainPI3K/PI4K
HgenePIK3CBchr3:138452203chr3:122667556ENST00000289153-622327_496350.01071.0DomainC2 PI3K-type
HgenePIK3CBchr3:138452203chr3:122667556ENST00000289153-622524_701350.01071.0DomainPIK helical
HgenePIK3CBchr3:138452203chr3:122667556ENST00000289153-622800_1067350.01071.0DomainPI3K/PI4K
HgenePIK3CBchr3:138452203chr3:122667556ENST00000477593-723327_496350.01071.0DomainC2 PI3K-type
HgenePIK3CBchr3:138452203chr3:122667556ENST00000477593-723524_701350.01071.0DomainPIK helical
HgenePIK3CBchr3:138452203chr3:122667556ENST00000477593-723800_1067350.01071.0DomainPI3K/PI4K
HgenePIK3CBchr3:138452202chr3:122667556ENST00000289153-622410_418350.01071.0MotifNote=Nuclear localization signal
HgenePIK3CBchr3:138452202chr3:122667556ENST00000477593-723410_418350.01071.0MotifNote=Nuclear localization signal
HgenePIK3CBchr3:138452203chr3:122667556ENST00000289153-622410_418350.01071.0MotifNote=Nuclear localization signal
HgenePIK3CBchr3:138452203chr3:122667556ENST00000477593-723410_418350.01071.0MotifNote=Nuclear localization signal
TgeneSEMA5Bchr3:138452202chr3:122667556ENST000003575991231_103641.3333333333333361152.0Topological domainExtracellular
TgeneSEMA5Bchr3:138452202chr3:122667556ENST000004510551231_103695.333333333333331206.0Topological domainExtracellular
TgeneSEMA5Bchr3:138452203chr3:122667556ENST000003575991231_103641.3333333333333361152.0Topological domainExtracellular
TgeneSEMA5Bchr3:138452203chr3:122667556ENST000004510551231_103695.333333333333331206.0Topological domainExtracellular


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Fusion Gene Sequence for PIK3CB-SEMA5B


check button For in-frame fusion transcripts, we provide the fusion transcript sequences and fusion amino acid sequences. To have fusion amino acid sequence, we ran ORFfinder and chose the longest ORF among the all predicted ones.
>In-frame_ENST00000477593_ENST00000357599_TCGA-G7-6793-01A_PIK3CB_chr3_138452203_-_SEMA5B_chr3_122667556_length(transcript)=5405nt_BP=1124nt
GGGCTGCTGACTGCGCACTTGGAATAGTAGCAGGCGGCGGCGGCGGAACGCCAGGCAGGGCCCCGTGGTTATGAATGTGCTTCAGTTTCA
TAATGCCTCCTGCTATGGCAGACATCCTTGACATCTGGGCGGTGGATTCACAGATAGCATCTGATGGCTCCATACCTGTGGATTTCCTTT
TGCCCACTGGGATTTATATCCAGTTGGAGGTACCTCGGGAAGCTACCATTTCTTATATTAAGCAGATGTTATGGAAGCAAGTTCACAATT
ACCCAATGTTCAACCTCCTTATGGATATTGACTCCTATATGTTTGCATGTGTGAATCAGACTGCTGTATATGAGGAGCTTGAAGATGAAA
CACGAAGACTCTGTGATGTCAGACCTTTTCTTCCAGTTCTCAAATTAGTGACAAGAAGTTGTGACCCAGGGGAAAAATTAGACTCAAAAA
TTGGAGTCCTTATAGGAAAAGGTCTGCATGAATTTGATTCCTTGAAGGATCCTGAAGTAAATGAATTTCGAAGAAAAATGCGCAAATTCA
GCGAGGAAAAAATCCTGTCACTTGTGGGATTGTCTTGGATGGACTGGCTAAAACAAACATATCCACCAGAGCATGAACCATCCATCCCTG
AAAACTTAGAAGATAAACTTTATGGGGGAAAGCTCATCGTAGCTGTTCATTTTGAAAACTGCCAGGACGTGTTTAGCTTTCAAGTGTCTC
CTAATATGAATCCTATCAAAGTAAATGAATTGGCAATCCAAAAACGTTTGACTATTCATGGGAAGGAAGATGAAGTTAGCCCCTATGATT
ATGTGTTGCAAGTCAGCGGGAGAGTAGAATATGTTTTTGGTGATCATCCACTAATTCAGTTCCAGTATATCCGGAACTGTGTGATGAACA
GAGCCCTGCCCCATTTTATACTTGTGGAATGCTGCAAGATCAAGAAAATGTATGAACAAGAAATGATTGCCATAGAGGCTGCCATAAATC
GAAATTCATCTAATCTTCCTCTTCCATTACCACCAAAGAAAACACGAATTATTTCTCATGTTTGGGAAAATAACAACCCTTTCCAAATTG
TCTTGGTTAAGGGAAATAAACTTAACACAGAGGAAACTGTAAAAGTCTTCTCCCCTGTCTGCCTCCCGGAGCTAGGACTGCAGAGGGGCC
TATCATGGTGCTTGCAGGCCCCCTGGCTGTCTCGCTGTTGCTGCCCAGCCTCACACTGCTGGTGTCCCACCTCTCCAGCTCCCAGGATGT
CTCCAGTGAGCCCAGCAGTGAGCAGCAGCTGTGCGCCCTTAGCAAGCACCCCACCGTGGCCTTTGAAGACCTGCAGCCGTGGGTCTCTAA
CTTCACCTACCCTGGAGCCCGGGATTTCTCCCAGCTGGCTTTGGACCCCTCCGGGAACCAGCTCATCGTGGGAGCCAGGAACTACCTCTT
CAGACTCAGCCTTGCCAATGTCTCTCTTCTTCAGGCCACAGAGTGGGCCTCCAGTGAGGACACGCGCCGCTCCTGCCAAAGCAAAGGGAA
GACTGAGGAGGAGTGTCAGAACTACGTGCGAGTCCTGATCGTCGCCGGCCGGAAGGTGTTCATGTGTGGAACCAATGCCTTTTCCCCCAT
GTGCACCAGCAGACAGGTGGGGAACCTCAGCCGGACTATTGAGAAGATCAATGGTGTGGCCCGCTGCCCCTATGACCCACGCCACAACTC
CACAGCTGTCATCTCCTCCCAGGGGGAGCTCTATGCAGCCACGGTCATCGACTTCTCAGGTCGGGACCCTGCCATCTACCGCAGCCTGGG
CAGTGGGCCACCGCTTCGCACTGCCCAATATAACTCCAAGTGGCTTAATGAGCCAAACTTCGTGGCAGCCTATGATATTGGGCTGTTTGC
ATACTTCTTCCTGCGGGAGAACGCAGTGGAGCACGACTGTGGACGCACCGTGTACTCTCGCGTGGCCCGCGTGTGCAAGAATGACGTGGG
GGGCCGATTCCTGCTGGAGGACACATGGACCACATTCATGAAGGCCCGGCTCAACTGCTCCCGCCCGGGCGAGGTCCCCTTCTACTATAA
CGAGCTGCAGAGTGCCTTCCACTTGCCGGAGCAGGACCTCATCTATGGAGTTTTCACAACCAACGTAAACAGCATCGCGGCTTCTGCTGT
CTGCGCCTTCAACCTCAGTGCTATCTCCCAGGCTTTCAATGGCCCATTTCGCTACCAGGAGAACCCCAGGGCTGCCTGGCTCCCCATAGC
CAACCCCATCCCCAATTTCCAGTGTGGCACCCTGCCTGAGACCGGTCCCAACGAGAACCTGACGGAGCGCAGCCTGCAGGACGCGCAGCG
CCTCTTCCTGATGAGCGAGGCCGTGCAGCCGGTGACACCCGAGCCCTGTGTCACCCAGGACAGCGTGCGCTTCTCACACCTCGTGGTGGA
CCTGGTGCAGGCTAAAGACACGCTCTACCATGTACTCTACATTGGCACCGAGTCGGGCACCATCCTGAAGGCGCTGTCCACGGCGAGCCG
CAGCCTCCACGGCTGCTACCTGGAGGAGCTGCACGTGCTGCCCCCCGGGCGCCGCGAGCCCCTGCGCAGCCTGCGCATCCTGCACAGCGC
CCGCGCGCTCTTCGTGGGGCTGAGAGACGGCGTCCTGCGGGTCCCACTGGAGAGGTGCGCCGCCTACCGCAGCCAGGGGGCATGCCTGGG
GGCCCGGGACCCGTACTGTGGCTGGGACGGGAAGCAGCAACGTTGCAGCACACTCGAGGACAGCTCCAACATGAGCCTCTGGACCCAGAA
CATCACCGCCTGTCCTGTGCGGAATGTGACACGGGATGGGGGCTTCGGCCCATGGTCACCATGGCAACCATGTGAGCACTTGGATGGGGA
CAACTCAGGCTCTTGCCTGTGTCGAGCTCGATCCTGTGATTCCCCTCGACCCCGCTGTGGGGGCCTTGACTGCCTGGGGCCAGCCATCCA
CATCGCCAACTGCTCCAGGAATGGGGCGTGGACCCCGTGGTCATCGTGGGCGCTGTGCAGCACGTCCTGTGGCATCGGCTTCCAGGTCCG
CCAGCGAAGTTGCAGCAACCCTGCTCCCCGCCACGGGGGCCGCATCTGCGTGGGCAAGAGCCGGGAGGAACGGTTCTGTAATGAGAACAC
GCCTTGCCCGGTGCCCATCTTCTGGGCTTCCTGGGGCTCCTGGAGCAAGTGCAGCAGCAACTGTGGAGGGGGCATGCAGTCGCGGCGTCG
GGCCTGCGAGAACGGCAACTCCTGCCTGGGCTGCGGCGTGGAGTTCAAGACGTGCAACCCCGAGGGCTGCCCCGAAGTGCGGCGCAACAC
CCCCTGGACGCCGTGGCTGCCCGTGAACGTGACGCAGGGCGGGGCACGGCAGGAGCAGCGGTTCCGCTTCACCTGCCGCGCGCCCCTTGC
AGACCCGCACGGCCTGCAGTTCGGCAGGAGAAGGACCGAGACGAGGACCTGTCCCGCGGACGGCTCCGGCTCCTGCGACACCGACGCCCT
GGTGGAGGTCCTCCTGCGCAGCGGGAGCACCTCCCCGCACACGGTGAGCGGGGGCTGGGCCGCCTGGGGCCCGTGGTCGTCCTGCTCCCG
GGACTGCGAGCTGGGCTTCCGCGTCCGCAAGAGAACGTGCACTAACCCGGAGCCCCGCAACGGGGGCCTGCCCTGCGTGGGCGATGCTGC
CGAGTACCAGGACTGCAACCCCCAGGCTTGCCCAGTTCGGGGTGCTTGGTCCTGCTGGACCTCATGGTCTCCATGCTCAGCTTCCTGTGG
TGGGGGTCACTATCAACGCACCCGTTCCTGCACCAGCCCCGCACCCTCCCCAGGTGAGGACATCTGTCTCGGGCTGCACACGGAGGAGGC
ACTATGTGCCACACAGGCCTGCCCAGAAGGCTGGTCGCCCTGGTCTGAGTGGAGTAAGTGCACTGACGACGGAGCCCAGAGCCGAAGCCG
GCACTGTGAGGAGCTCCTCCCAGGGTCCAGCGCCTGTGCTGGAAACAGCAGCCAGAGCCGCCCCTGCCCCTACAGCGAGATTCCCGTCAT
CCTGCCAGCCTCCAGCATGGAGGAGGCCACCGACTGTGCAGGGTTCAATCTCATCCACTTGGTGGCCACGGGCATCTCCTGCTTCTTGGG
CTCTGGGCTCCTGACCCTAGCAGTGTACCTGTCTTGCCAGCACTGCCAGCGTCAGTCCCAGGAGTCCACACTGGTCCATCCTGCCACCCC
CAACCATTTGCACTACAAGGGCGGAGGCACCCCGAAGAATGAAAAGTACACACCCATGGAATTCAAGACCCTGAACAAGAATAACTTGAT
CCCTGATGACAGAGCCAACTTCTACCCATTGCAGCAGACCAATGTGTACACGACTACTTACTACCCAAGCCCCCTGAACAAACACAGCTT
CCGGCCCGAGGCCTCACCTGGACAACGGTGCTTCCCCAACAGCTGATACCGCCGTCCTGGGGACTTGGGCTTCTTGCCTTCATAAGGCAC
AGAGCAGATGGAGATGGGACAGTGGAGCCAGTTTGGTTTTCTCCCTCTGCACTAGGCCAAGAACTTGCTGCCTTGCCTGTGGGGGGTCCC
ATCCGGCTTCAGAGAGCTCTGGCTGGCATTGACCATGGGGGAAAGGGCTGGTTTCAGGCTGACATATGGCCGCAGGTCCAGTTCAGCCCA
GGTCTCTCATGGTTATCTTCCAACCCACTGTCACGCTGACACTATGCTGCCATGCCTGGGCTGTGGACCTACTGGGCATTTGAGGAATTG
GAGAATGGAGATGGCAAGAGGGCAGGCTTTTAAGTTTGGGTTGGAGACAACTTCCTGTGGCCCCCACAAGCTGAGTCTGGCCTTCTCCAG
CTGGCCCCAAAAAAGGCCTTTGCTACATCCTGATTATCTCTGAAAGTAATCAATCAAGTGGCTCCAGTAGCTCTGGATTTTCTGCCAGGG
CTGGGCCATTGTGGTGCTGCCCCAGTATGACATGGGACCAAGGCCAGCGCAGGTTATCCACCTCTGCCTGGAAGTCTATACTCTACCCAG
GGCATCCCTCTGGTCAGAGGCAGTGAGTACTGGGAACTGGAGGCTGACCTGTGCTTAGAAGTCCTTTAATCTGGGCTGGTACAGGCCTCA
GCCTTGCCCTCAATGCACGAAAGGTGGCCCAGGAGAGAGGATCAATGCCATAGGAGGCAGAAGTCTGGCCTCTGTGCCTCTATGGAGACT
ATCTTCCAGTTGCTGCTCAACAGAGTTGTTGGCTGAGACCTGCTTGGGAGTCTCTGCTGGCCCTTCATCTGTTCAGGAACACACACACAC
ACACACTCACACACGCACACACAATCACAATTTGCTACAGCAACAAAAAAGACATTGGGCTGTGGCATTATTAATTAAAGATGATATCCA

>In-frame_ENST00000477593_ENST00000357599_TCGA-G7-6793-01A_PIK3CB_chr3_138452203_-_SEMA5B_chr3_122667556_length(amino acids)=1105AA_start in transcript=1138_stop in transcript=4455
MPPGARTAEGPIMVLAGPLAVSLLLPSLTLLVSHLSSSQDVSSEPSSEQQLCALSKHPTVAFEDLQPWVSNFTYPGARDFSQLALDPSGN
QLIVGARNYLFRLSLANVSLLQATEWASSEDTRRSCQSKGKTEEECQNYVRVLIVAGRKVFMCGTNAFSPMCTSRQVGNLSRTIEKINGV
ARCPYDPRHNSTAVISSQGELYAATVIDFSGRDPAIYRSLGSGPPLRTAQYNSKWLNEPNFVAAYDIGLFAYFFLRENAVEHDCGRTVYS
RVARVCKNDVGGRFLLEDTWTTFMKARLNCSRPGEVPFYYNELQSAFHLPEQDLIYGVFTTNVNSIAASAVCAFNLSAISQAFNGPFRYQ
ENPRAAWLPIANPIPNFQCGTLPETGPNENLTERSLQDAQRLFLMSEAVQPVTPEPCVTQDSVRFSHLVVDLVQAKDTLYHVLYIGTESG
TILKALSTASRSLHGCYLEELHVLPPGRREPLRSLRILHSARALFVGLRDGVLRVPLERCAAYRSQGACLGARDPYCGWDGKQQRCSTLE
DSSNMSLWTQNITACPVRNVTRDGGFGPWSPWQPCEHLDGDNSGSCLCRARSCDSPRPRCGGLDCLGPAIHIANCSRNGAWTPWSSWALC
STSCGIGFQVRQRSCSNPAPRHGGRICVGKSREERFCNENTPCPVPIFWASWGSWSKCSSNCGGGMQSRRRACENGNSCLGCGVEFKTCN
PEGCPEVRRNTPWTPWLPVNVTQGGARQEQRFRFTCRAPLADPHGLQFGRRRTETRTCPADGSGSCDTDALVEVLLRSGSTSPHTVSGGW
AAWGPWSSCSRDCELGFRVRKRTCTNPEPRNGGLPCVGDAAEYQDCNPQACPVRGAWSCWTSWSPCSASCGGGHYQRTRSCTSPAPSPGE
DICLGLHTEEALCATQACPEGWSPWSEWSKCTDDGAQSRSRHCEELLPGSSACAGNSSQSRPCPYSEIPVILPASSMEEATDCAGFNLIH
LVATGISCFLGSGLLTLAVYLSCQHCQRQSQESTLVHPATPNHLHYKGGGTPKNEKYTPMEFKTLNKNNLIPDDRANFYPLQQTNVYTTT

--------------------------------------------------------------
>In-frame_ENST00000477593_ENST00000195173_TCGA-G7-6793-01A_PIK3CB_chr3_138452203_-_SEMA5B_chr3_122667556_length(transcript)=5528nt_BP=1124nt
GGGCTGCTGACTGCGCACTTGGAATAGTAGCAGGCGGCGGCGGCGGAACGCCAGGCAGGGCCCCGTGGTTATGAATGTGCTTCAGTTTCA
TAATGCCTCCTGCTATGGCAGACATCCTTGACATCTGGGCGGTGGATTCACAGATAGCATCTGATGGCTCCATACCTGTGGATTTCCTTT
TGCCCACTGGGATTTATATCCAGTTGGAGGTACCTCGGGAAGCTACCATTTCTTATATTAAGCAGATGTTATGGAAGCAAGTTCACAATT
ACCCAATGTTCAACCTCCTTATGGATATTGACTCCTATATGTTTGCATGTGTGAATCAGACTGCTGTATATGAGGAGCTTGAAGATGAAA
CACGAAGACTCTGTGATGTCAGACCTTTTCTTCCAGTTCTCAAATTAGTGACAAGAAGTTGTGACCCAGGGGAAAAATTAGACTCAAAAA
TTGGAGTCCTTATAGGAAAAGGTCTGCATGAATTTGATTCCTTGAAGGATCCTGAAGTAAATGAATTTCGAAGAAAAATGCGCAAATTCA
GCGAGGAAAAAATCCTGTCACTTGTGGGATTGTCTTGGATGGACTGGCTAAAACAAACATATCCACCAGAGCATGAACCATCCATCCCTG
AAAACTTAGAAGATAAACTTTATGGGGGAAAGCTCATCGTAGCTGTTCATTTTGAAAACTGCCAGGACGTGTTTAGCTTTCAAGTGTCTC
CTAATATGAATCCTATCAAAGTAAATGAATTGGCAATCCAAAAACGTTTGACTATTCATGGGAAGGAAGATGAAGTTAGCCCCTATGATT
ATGTGTTGCAAGTCAGCGGGAGAGTAGAATATGTTTTTGGTGATCATCCACTAATTCAGTTCCAGTATATCCGGAACTGTGTGATGAACA
GAGCCCTGCCCCATTTTATACTTGTGGAATGCTGCAAGATCAAGAAAATGTATGAACAAGAAATGATTGCCATAGAGGCTGCCATAAATC
GAAATTCATCTAATCTTCCTCTTCCATTACCACCAAAGAAAACACGAATTATTTCTCATGTTTGGGAAAATAACAACCCTTTCCAAATTG
TCTTGGTTAAGGGAAATAAACTTAACACAGAGGAAACTGTAAAAGTCTTCTCCCCTGTCTGCCTCCCGGAGCTAGGACTGCAGAGGGGCC
TATCATGGTGCTTGCAGGCCCCCTGGCTGTCTCGCTGTTGCTGCCCAGCCTCACACTGCTGGTGTCCCACCTCTCCAGCTCCCAGGATGT
CTCCAGTGAGCCCAGCAGTGAGCAGCAGCTGTGCGCCCTTAGCAAGCACCCCACCGTGGCCTTTGAAGACCTGCAGCCGTGGGTCTCTAA
CTTCACCTACCCTGGAGCCCGGGATTTCTCCCAGCTGGCTTTGGACCCCTCCGGGAACCAGCTCATCGTGGGAGCCAGGAACTACCTCTT
CAGACTCAGCCTTGCCAATGTCTCTCTTCTTCAGGCCACAGAGTGGGCCTCCAGTGAGGACACGCGCCGCTCCTGCCAAAGCAAAGGGAA
GACTGAGGAGGAGTGTCAGAACTACGTGCGAGTCCTGATCGTCGCCGGCCGGAAGGTGTTCATGTGTGGAACCAATGCCTTTTCCCCCAT
GTGCACCAGCAGACAGGTGGGGAACCTCAGCCGGACTATTGAGAAGATCAATGGTGTGGCCCGCTGCCCCTATGACCCACGCCACAACTC
CACAGCTGTCATCTCCTCCCAGGGGGAGCTCTATGCAGCCACGGTCATCGACTTCTCAGGTCGGGACCCTGCCATCTACCGCAGCCTGGG
CAGTGGGCCACCGCTTCGCACTGCCCAATATAACTCCAAGTGGCTTAATGAGCCAAACTTCGTGGCAGCCTATGATATTGGGCTGTTTGC
ATACTTCTTCCTGCGGGAGAACGCAGTGGAGCACGACTGTGGACGCACCGTGTACTCTCGCGTGGCCCGCGTGTGCAAGAATGACGTGGG
GGGCCGATTCCTGCTGGAGGACACATGGACCACATTCATGAAGGCCCGGCTCAACTGCTCCCGCCCGGGCGAGGTCCCCTTCTACTATAA
CGAGCTGCAGAGTGCCTTCCACTTGCCGGAGCAGGACCTCATCTATGGAGTTTTCACAACCAACGTAAACAGCATCGCGGCTTCTGCTGT
CTGCGCCTTCAACCTCAGTGCTATCTCCCAGGCTTTCAATGGCCCATTTCGCTACCAGGAGAACCCCAGGGCTGCCTGGCTCCCCATAGC
CAACCCCATCCCCAATTTCCAGTGTGGCACCCTGCCTGAGACCGGTCCCAACGAGAACCTGACGGAGCGCAGCCTGCAGGACGCGCAGCG
CCTCTTCCTGATGAGCGAGGCCGTGCAGCCGGTGACACCCGAGCCCTGTGTCACCCAGGACAGCGTGCGCTTCTCACACCTCGTGGTGGA
CCTGGTGCAGGCTAAAGACACGCTCTACCATGTACTCTACATTGGCACCGAGTCGGGCACCATCCTGAAGGCGCTGTCCACGGCGAGCCG
CAGCCTCCACGGCTGCTACCTGGAGGAGCTGCACGTGCTGCCCCCCGGGCGCCGCGAGCCCCTGCGCAGCCTGCGCATCCTGCACAGCGC
CCGCGCGCTCTTCGTGGGGCTGAGAGACGGCGTCCTGCGGGTCCCACTGGAGAGGTGCGCCGCCTACCGCAGCCAGGGGGCATGCCTGGG
GGCCCGGGACCCGTACTGTGGCTGGGACGGGAAGCAGCAACGTTGCAGCACACTCGAGGACAGCTCCAACATGAGCCTCTGGACCCAGAA
CATCACCGCCTGTCCTGTGCGGAATGTGACACGGGATGGGGGCTTCGGCCCATGGTCACCATGGCAACCATGTGAGCACTTGGATGGGGA
CAACTCAGGCTCTTGCCTGTGTCGAGCTCGATCCTGTGATTCCCCTCGACCCCGCTGTGGGGGCCTTGACTGCCTGGGGCCAGCCATCCA
CATCGCCAACTGCTCCAGGAATGGGGCGTGGACCCCGTGGTCATCGTGGGCGCTGTGCAGCACGTCCTGTGGCATCGGCTTCCAGGTCCG
CCAGCGAAGTTGCAGCAACCCTGCTCCCCGCCACGGGGGCCGCATCTGCGTGGGCAAGAGCCGGGAGGAACGGTTCTGTAATGAGAACAC
GCCTTGCCCGGTGCCCATCTTCTGGGCTTCCTGGGGCTCCTGGAGCAAGTGCAGCAGCAACTGTGGAGGGGGCATGCAGTCGCGGCGTCG
GGCCTGCGAGAACGGCAACTCCTGCCTGGGCTGCGGCGAGTTCAAGACGTGCAACCCCGAGGGCTGCCCCGAAGTGCGGCGCAACACCCC
CTGGACGCCGTGGCTGCCCGTGAACGTGACGCAGGGCGGGGCACGGCAGGAGCAGCGGTTCCGCTTCACCTGCCGCGCGCCCCTTGCAGA
CCCGCACGGCCTGCAGTTCGGCAGGAGAAGGACCGAGACGAGGACCTGTCCCGCGGACGGCTCCGGCTCCTGCGACACCGACGCCCTGGT
GGAGGTCCTCCTGCGCAGCGGGAGCACCTCCCCGCACACGGTGAGCGGGGGCTGGGCCGCCTGGGGCCCGTGGTCGTCCTGCTCCCGGGA
CTGCGAGCTGGGCTTCCGCGTCCGCAAGAGAACGTGCACTAACCCGGAGCCCCGCAACGGGGGCCTGCCCTGCGTGGGCGATGCTGCCGA
GTACCAGGACTGCAACCCCCAGGCTTGCCCAGTTCGGGGTGCTTGGTCCTGCTGGACCTCATGGTCTCCATGCTCAGCTTCCTGTGGTGG
GGGTCACTATCAACGCACCCGTTCCTGCACCAGCCCCGCACCCTCCCCAGGTGAGGACATCTGTCTCGGGCTGCACACGGAGGAGGCACT
ATGTGCCACACAGGCCTGCCCAGGCTGGTCGCCCTGGTCTGAGTGGAGTAAGTGCACTGACGACGGAGCCCAGAGCCGAAGCCGGCACTG
TGAGGAGCTCCTCCCAGGGTCCAGCGCCTGTGCTGGAAACAGCAGCCAGAGCCGCCCCTGCCCCTACAGCGAGATTCCCGTCATCCTGCC
AGCCTCCAGCATGGAGGAGGCCACCGACTGTGCAGGTAAAAGAAACCGGACCTACCTCATGCTGCGGTCCTCCCAGCCCTCCGGCACCCC
ACTCCAAAGTCTGGACTCTTTCCACATCCTGCTCCAGACAGCCAAGCTTTGTTGGGGTCCCCACTGCTTTGAGATGGGTTCAATCTCATC
CACTTGGTGGCCACGGGCATCTCCTGCTTCTTGGGCTCTGGGCTCCTGACCCTAGCAGTGTACCTGTCTTGCCAGCACTGCCAGCGTCAG
TCCCAGGAGTCCACACTGGTCCATCCTGCCACCCCCAACCATTTGCACTACAAGGGCGGAGGCACCCCGAAGAATGAAAAGTACACACCC
ATGGAATTCAAGACCCTGAACAAGAATAACTTGATCCCTGATGACAGAGCCAACTTCTACCCATTGCAGCAGACCAATGTGTACACGACT
ACTTACTACCCAAGCCCCCTGAACAAACACAGCTTCCGGCCCGAGGCCTCACCTGGACAACGGTGCTTCCCCAACAGCTGATACCGCCGT
CCTGGGGACTTGGGCTTCTTGCCTTCATAAGGCACAGAGCAGATGGAGATGGGACAGTGGAGCCAGTTTGGTTTTCTCCCTCTGCACTAG
GCCAAGAACTTGCTGCCTTGCCTGTGGGGGGTCCCATCCGGCTTCAGAGAGCTCTGGCTGGCATTGACCATGGGGGAAAGGGCTGGTTTC
AGGCTGACATATGGCCGCAGGTCCAGTTCAGCCCAGGTCTCTCATGGTTATCTTCCAACCCACTGTCACGCTGACACTATGCTGCCATGC
CTGGGCTGTGGACCTACTGGGCATTTGAGGAATTGGAGAATGGAGATGGCAAGAGGGCAGGCTTTTAAGTTTGGGTTGGAGACAACTTCC
TGTGGCCCCCACAAGCTGAGTCTGGCCTTCTCCAGCTGGCCCCAAAAAAGGCCTTTGCTACATCCTGATTATCTCTGAAAGTAATCAATC
AAGTGGCTCCAGTAGCTCTGGATTTTCTGCCAGGGCTGGGCCATTGTGGTGCTGCCCCAGTATGACATGGGACCAAGGCCAGCGCAGGTT
ATCCACCTCTGCCTGGAAGTCTATACTCTACCCAGGGCATCCCTCTGGTCAGAGGCAGTGAGTACTGGGAACTGGAGGCTGACCTGTGCT
TAGAAGTCCTTTAATCTGGGCTGGTACAGGCCTCAGCCTTGCCCTCAATGCACGAAAGGTGGCCCAGGAGAGAGGATCAATGCCATAGGA
GGCAGAAGTCTGGCCTCTGTGCCTCTATGGAGACTATCTTCCAGTTGCTGCTCAACAGAGTTGTTGGCTGAGACCTGCTTGGGAGTCTCT
GCTGGCCCTTCATCTGTTCAGGAACACACACACACACACACTCACACACGCACACACAATCACAATTTGCTACAGCAACAAAAAAGACAT

>In-frame_ENST00000477593_ENST00000195173_TCGA-G7-6793-01A_PIK3CB_chr3_138452203_-_SEMA5B_chr3_122667556_length(amino acids)=1046AA_start in transcript=1138_stop in transcript=4278
MPPGARTAEGPIMVLAGPLAVSLLLPSLTLLVSHLSSSQDVSSEPSSEQQLCALSKHPTVAFEDLQPWVSNFTYPGARDFSQLALDPSGN
QLIVGARNYLFRLSLANVSLLQATEWASSEDTRRSCQSKGKTEEECQNYVRVLIVAGRKVFMCGTNAFSPMCTSRQVGNLSRTIEKINGV
ARCPYDPRHNSTAVISSQGELYAATVIDFSGRDPAIYRSLGSGPPLRTAQYNSKWLNEPNFVAAYDIGLFAYFFLRENAVEHDCGRTVYS
RVARVCKNDVGGRFLLEDTWTTFMKARLNCSRPGEVPFYYNELQSAFHLPEQDLIYGVFTTNVNSIAASAVCAFNLSAISQAFNGPFRYQ
ENPRAAWLPIANPIPNFQCGTLPETGPNENLTERSLQDAQRLFLMSEAVQPVTPEPCVTQDSVRFSHLVVDLVQAKDTLYHVLYIGTESG
TILKALSTASRSLHGCYLEELHVLPPGRREPLRSLRILHSARALFVGLRDGVLRVPLERCAAYRSQGACLGARDPYCGWDGKQQRCSTLE
DSSNMSLWTQNITACPVRNVTRDGGFGPWSPWQPCEHLDGDNSGSCLCRARSCDSPRPRCGGLDCLGPAIHIANCSRNGAWTPWSSWALC
STSCGIGFQVRQRSCSNPAPRHGGRICVGKSREERFCNENTPCPVPIFWASWGSWSKCSSNCGGGMQSRRRACENGNSCLGCGEFKTCNP
EGCPEVRRNTPWTPWLPVNVTQGGARQEQRFRFTCRAPLADPHGLQFGRRRTETRTCPADGSGSCDTDALVEVLLRSGSTSPHTVSGGWA
AWGPWSSCSRDCELGFRVRKRTCTNPEPRNGGLPCVGDAAEYQDCNPQACPVRGAWSCWTSWSPCSASCGGGHYQRTRSCTSPAPSPGED
ICLGLHTEEALCATQACPGWSPWSEWSKCTDDGAQSRSRHCEELLPGSSACAGNSSQSRPCPYSEIPVILPASSMEEATDCAGKRNRTYL

--------------------------------------------------------------
>In-frame_ENST00000477593_ENST00000451055_TCGA-G7-6793-01A_PIK3CB_chr3_138452203_-_SEMA5B_chr3_122667556_length(transcript)=4935nt_BP=1124nt
GGGCTGCTGACTGCGCACTTGGAATAGTAGCAGGCGGCGGCGGCGGAACGCCAGGCAGGGCCCCGTGGTTATGAATGTGCTTCAGTTTCA
TAATGCCTCCTGCTATGGCAGACATCCTTGACATCTGGGCGGTGGATTCACAGATAGCATCTGATGGCTCCATACCTGTGGATTTCCTTT
TGCCCACTGGGATTTATATCCAGTTGGAGGTACCTCGGGAAGCTACCATTTCTTATATTAAGCAGATGTTATGGAAGCAAGTTCACAATT
ACCCAATGTTCAACCTCCTTATGGATATTGACTCCTATATGTTTGCATGTGTGAATCAGACTGCTGTATATGAGGAGCTTGAAGATGAAA
CACGAAGACTCTGTGATGTCAGACCTTTTCTTCCAGTTCTCAAATTAGTGACAAGAAGTTGTGACCCAGGGGAAAAATTAGACTCAAAAA
TTGGAGTCCTTATAGGAAAAGGTCTGCATGAATTTGATTCCTTGAAGGATCCTGAAGTAAATGAATTTCGAAGAAAAATGCGCAAATTCA
GCGAGGAAAAAATCCTGTCACTTGTGGGATTGTCTTGGATGGACTGGCTAAAACAAACATATCCACCAGAGCATGAACCATCCATCCCTG
AAAACTTAGAAGATAAACTTTATGGGGGAAAGCTCATCGTAGCTGTTCATTTTGAAAACTGCCAGGACGTGTTTAGCTTTCAAGTGTCTC
CTAATATGAATCCTATCAAAGTAAATGAATTGGCAATCCAAAAACGTTTGACTATTCATGGGAAGGAAGATGAAGTTAGCCCCTATGATT
ATGTGTTGCAAGTCAGCGGGAGAGTAGAATATGTTTTTGGTGATCATCCACTAATTCAGTTCCAGTATATCCGGAACTGTGTGATGAACA
GAGCCCTGCCCCATTTTATACTTGTGGAATGCTGCAAGATCAAGAAAATGTATGAACAAGAAATGATTGCCATAGAGGCTGCCATAAATC
GAAATTCATCTAATCTTCCTCTTCCATTACCACCAAAGAAAACACGAATTATTTCTCATGTTTGGGAAAATAACAACCCTTTCCAAATTG
TCTTGGTTAAGGGAAATAAACTTAACACAGAGGAAACTGTAAAAGTCTTCTCCCCTGTCTGCCTCCCGGAGCTAGGACTGCAGAGGGGCC
TATCATGGTGCTTGCAGGCCCCCTGGCTGTCTCGCTGTTGCTGCCCAGCCTCACACTGCTGGTGTCCCACCTCTCCAGCTCCCAGGATGT
CTCCAGTGAGCCCAGCAGTGAGCAGCAGCTGTGCGCCCTTAGCAAGCACCCCACCGTGGCCTTTGAAGACCTGCAGCCGTGGGTCTCTAA
CTTCACCTACCCTGGAGCCCGGGATTTCTCCCAGCTGGCTTTGGACCCCTCCGGGAACCAGCTCATCGTGGGAGCCAGGAACTACCTCTT
CAGACTCAGCCTTGCCAATGTCTCTCTTCTTCAGGCCACAGAGTGGGCCTCCAGTGAGGACACGCGCCGCTCCTGCCAAAGCAAAGGGAA
GACTGAGGAGGAGTGTCAGAACTACGTGCGAGTCCTGATCGTCGCCGGCCGGAAGGTGTTCATGTGTGGAACCAATGCCTTTTCCCCCAT
GTGCACCAGCAGACAGGTGGGGAACCTCAGCCGGACTATTGAGAAGATCAATGGTGTGGCCCGCTGCCCCTATGACCCACGCCACAACTC
CACAGCTGTCATCTCCTCCCAGGGGGAGCTCTATGCAGCCACGGTCATCGACTTCTCAGGTCGGGACCCTGCCATCTACCGCAGCCTGGG
CAGTGGGCCACCGCTTCGCACTGCCCAATATAACTCCAAGTGGCTTAATGAGCCAAACTTCGTGGCAGCCTATGATATTGGGCTGTTTGC
ATACTTCTTCCTGCGGGAGAACGCAGTGGAGCACGACTGTGGACGCACCGTGTACTCTCGCGTGGCCCGCGTGTGCAAGAATGACGTGGG
GGGCCGATTCCTGCTGGAGGACACATGGACCACATTCATGAAGGCCCGGCTCAACTGCTCCCGCCCGGGCGAGGTCCCCTTCTACTATAA
CGAGCTGCAGAGTGCCTTCCACTTGCCGGAGCAGGACCTCATCTATGGAGTTTTCACAACCAACGTAAACAGCATCGCGGCTTCTGCTGT
CTGCGCCTTCAACCTCAGTGCTATCTCCCAGGCTTTCAATGGCCCATTTCGCTACCAGGAGAACCCCAGGGCTGCCTGGCTCCCCATAGC
CAACCCCATCCCCAATTTCCAGTGTGGCACCCTGCCTGAGACCGGTCCCAACGAGAACCTGACGGAGCGCAGCCTGCAGGACGCGCAGCG
CCTCTTCCTGATGAGCGAGGCCGTGCAGCCGGTGACACCCGAGCCCTGTGTCACCCAGGACAGCGTGCGCTTCTCACACCTCGTGGTGGA
CCTGGTGCAGGCTAAAGACACGCTCTACCATGTACTCTACATTGGCACCGAGTCGGGCACCATCCTGAAGGCGCTGTCCACGGCGAGCCG
CAGCCTCCACGGCTGCTACCTGGAGGAGCTGCACGTGCTGCCCCCCGGGCGCCGCGAGCCCCTGCGCAGCCTGCGCATCCTGCACAGCGC
CCGCGCGCTCTTCGTGGGGCTGAGAGACGGCGTCCTGCGGGTCCCACTGGAGAGGTGCGCCGCCTACCGCAGCCAGGGGGCATGCCTGGG
GGCCCGGGACCCGTACTGTGGCTGGGACGGGAAGCAGCAACGTTGCAGCACACTCGAGGACAGCTCCAACATGAGCCTCTGGACCCAGAA
CATCACCGCCTGTCCTGTGCGGAATGTGACACGGGATGGGGGCTTCGGCCCATGGTCACCATGGCAACCATGTGAGCACTTGGATGGGGA
CAACTCAGGCTCTTGCCTGTGTCGAGCTCGATCCTGTGATTCCCCTCGACCCCGCTGTGGGGGCCTTGACTGCCTGGGGCCAGCCATCCA
CATCGCCAACTGCTCCAGGAATGGGGCGTGGACCCCGTGGTCATCGTGGGCGCTGTGCAGCACGTCCTGTGGCATCGGCTTCCAGGTCCG
CCAGCGAAGTTGCAGCAACCCTGCTCCCCGCCACGGGGGCCGCATCTGCGTGGGCAAGAGCCGGGAGGAACGGTTCTGTAATGAGAACAC
GCCTTGCCCGGTGCCCATCTTCTGGGCTTCCTGGGGCTCCTGGAGCAAGTGCAGCAGCAACTGTGGAGGGGGCATGCAGTCGCGGCGTCG
GGCCTGCGAGAACGGCAACTCCTGCCTGGGCTGCGGCGTGGAGTTCAAGACGTGCAACCCCGAGGGCTGCCCCGAAGTGCGGCGCAACAC
CCCCTGGACGCCGTGGCTGCCCGTGAACGTGACGCAGGGCGGGGCACGGCAGGAGCAGCGGTTCCGCTTCACCTGCCGCGCGCCCCTTGC
AGACCCGCACGGCCTGCAGTTCGGCAGGAGAAGGACCGAGACGAGGACCTGTCCCGCGGACGGCTCCGGCTCCTGCGACACCGACGCCCT
GGTGGAGGTCCTCCTGCGCAGCGGGAGCACCTCCCCGCACACGGTGAGCGGGGGCTGGGCCGCCTGGGGCCCGTGGTCGTCCTGCTCCCG
GGACTGCGAGCTGGGCTTCCGCGTCCGCAAGAGAACGTGCACTAACCCGGAGCCCCGCAACGGGGGCCTGCCCTGCGTGGGCGATGCTGC
CGAGTACCAGGACTGCAACCCCCAGGCTTGCCCAGTTCGGGGTGCTTGGTCCTGCTGGACCTCATGGTCTCCATGCTCAGCTTCCTGTGG
TGGGGGTCACTATCAACGCACCCGTTCCTGCACCAGCCCCGCACCCTCCCCAGGTGAGGACATCTGTCTCGGGCTGCACACGGAGGAGGC
ACTATGTGCCACACAGGCCTGCCCAGAAGGCTGGTCGCCCTGGTCTGAGTGGAGTAAGTGCACTGACGACGGAGCCCAGAGCCGAAGCCG
GCACTGTGAGGAGCTCCTCCCAGGGTCCAGCGCCTGTGCTGGAAACAGCAGCCAGAGCCGCCCCTGCCCCTACAGCGAGATTCCCGTCAT
CCTGCCAGCCTCCAGCATGGAGGAGGCCACCGACTGTGCAGGGTTCAATCTCATCCACTTGGTGGCCACGGGCATCTCCTGCTTCTTGGG
CTCTGGGCTCCTGACCCTAGCAGTGTACCTGTCTTGCCAGCACTGCCAGCGTCAGTCCCAGGAGTCCACACTGGTCCATCCTGCCACCCC
CAACCATTTGCACTACAAGGGCGGAGGCACCCCGAAGAATGAAAAGTACACACCCATGGAATTCAAGACCCTGAACAAGAATAACTTGAT
CCCTGATGACAGAGCCAACTTCTACCCATTGCAGCAGACCAATGTGTACACGACTACTTACTACCCAAGCCCCCTGAACAAACACAGCTT
CCGGCCCGAGGCCTCACCTGGACAACGGTGCTTCCCCAACAGCTGATACCGCCGTCCTGGGGACTTGGGCTTCTTGCCTTCATAAGGCAC
AGAGCAGATGGAGATGGGACAGTGGAGCCAGTTTGGTTTTCTCCCTCTGCACTAGGCCAAGAACTTGCTGCCTTGCCTGTGGGGGGTCCC
ATCCGGCTTCAGAGAGCTCTGGCTGGCATTGACCATGGGGGAAAGGGCTGGTTTCAGGCTGACATATGGCCGCAGGTCCAGTTCAGCCCA
GGTCTCTCATGGTTATCTTCCAACCCACTGTCACGCTGACACTATGCTGCCATGCCTGGGCTGTGGACCTACTGGGCATTTGAGGAATTG
GAGAATGGAGATGGCAAGAGGGCAGGCTTTTAAGTTTGGGTTGGAGACAACTTCCTGTGGCCCCCACAAGCTGAGTCTGGCCTTCTCCAG

>In-frame_ENST00000477593_ENST00000451055_TCGA-G7-6793-01A_PIK3CB_chr3_138452203_-_SEMA5B_chr3_122667556_length(amino acids)=1105AA_start in transcript=1138_stop in transcript=4455
MPPGARTAEGPIMVLAGPLAVSLLLPSLTLLVSHLSSSQDVSSEPSSEQQLCALSKHPTVAFEDLQPWVSNFTYPGARDFSQLALDPSGN
QLIVGARNYLFRLSLANVSLLQATEWASSEDTRRSCQSKGKTEEECQNYVRVLIVAGRKVFMCGTNAFSPMCTSRQVGNLSRTIEKINGV
ARCPYDPRHNSTAVISSQGELYAATVIDFSGRDPAIYRSLGSGPPLRTAQYNSKWLNEPNFVAAYDIGLFAYFFLRENAVEHDCGRTVYS
RVARVCKNDVGGRFLLEDTWTTFMKARLNCSRPGEVPFYYNELQSAFHLPEQDLIYGVFTTNVNSIAASAVCAFNLSAISQAFNGPFRYQ
ENPRAAWLPIANPIPNFQCGTLPETGPNENLTERSLQDAQRLFLMSEAVQPVTPEPCVTQDSVRFSHLVVDLVQAKDTLYHVLYIGTESG
TILKALSTASRSLHGCYLEELHVLPPGRREPLRSLRILHSARALFVGLRDGVLRVPLERCAAYRSQGACLGARDPYCGWDGKQQRCSTLE
DSSNMSLWTQNITACPVRNVTRDGGFGPWSPWQPCEHLDGDNSGSCLCRARSCDSPRPRCGGLDCLGPAIHIANCSRNGAWTPWSSWALC
STSCGIGFQVRQRSCSNPAPRHGGRICVGKSREERFCNENTPCPVPIFWASWGSWSKCSSNCGGGMQSRRRACENGNSCLGCGVEFKTCN
PEGCPEVRRNTPWTPWLPVNVTQGGARQEQRFRFTCRAPLADPHGLQFGRRRTETRTCPADGSGSCDTDALVEVLLRSGSTSPHTVSGGW
AAWGPWSSCSRDCELGFRVRKRTCTNPEPRNGGLPCVGDAAEYQDCNPQACPVRGAWSCWTSWSPCSASCGGGHYQRTRSCTSPAPSPGE
DICLGLHTEEALCATQACPEGWSPWSEWSKCTDDGAQSRSRHCEELLPGSSACAGNSSQSRPCPYSEIPVILPASSMEEATDCAGFNLIH
LVATGISCFLGSGLLTLAVYLSCQHCQRQSQESTLVHPATPNHLHYKGGGTPKNEKYTPMEFKTLNKNNLIPDDRANFYPLQQTNVYTTT

--------------------------------------------------------------
>In-frame_ENST00000477593_ENST00000357599_TCGA-G7-6793_PIK3CB_chr3_138452202_-_SEMA5B_chr3_122667556_length(transcript)=5405nt_BP=1124nt
GGGCTGCTGACTGCGCACTTGGAATAGTAGCAGGCGGCGGCGGCGGAACGCCAGGCAGGGCCCCGTGGTTATGAATGTGCTTCAGTTTCA
TAATGCCTCCTGCTATGGCAGACATCCTTGACATCTGGGCGGTGGATTCACAGATAGCATCTGATGGCTCCATACCTGTGGATTTCCTTT
TGCCCACTGGGATTTATATCCAGTTGGAGGTACCTCGGGAAGCTACCATTTCTTATATTAAGCAGATGTTATGGAAGCAAGTTCACAATT
ACCCAATGTTCAACCTCCTTATGGATATTGACTCCTATATGTTTGCATGTGTGAATCAGACTGCTGTATATGAGGAGCTTGAAGATGAAA
CACGAAGACTCTGTGATGTCAGACCTTTTCTTCCAGTTCTCAAATTAGTGACAAGAAGTTGTGACCCAGGGGAAAAATTAGACTCAAAAA
TTGGAGTCCTTATAGGAAAAGGTCTGCATGAATTTGATTCCTTGAAGGATCCTGAAGTAAATGAATTTCGAAGAAAAATGCGCAAATTCA
GCGAGGAAAAAATCCTGTCACTTGTGGGATTGTCTTGGATGGACTGGCTAAAACAAACATATCCACCAGAGCATGAACCATCCATCCCTG
AAAACTTAGAAGATAAACTTTATGGGGGAAAGCTCATCGTAGCTGTTCATTTTGAAAACTGCCAGGACGTGTTTAGCTTTCAAGTGTCTC
CTAATATGAATCCTATCAAAGTAAATGAATTGGCAATCCAAAAACGTTTGACTATTCATGGGAAGGAAGATGAAGTTAGCCCCTATGATT
ATGTGTTGCAAGTCAGCGGGAGAGTAGAATATGTTTTTGGTGATCATCCACTAATTCAGTTCCAGTATATCCGGAACTGTGTGATGAACA
GAGCCCTGCCCCATTTTATACTTGTGGAATGCTGCAAGATCAAGAAAATGTATGAACAAGAAATGATTGCCATAGAGGCTGCCATAAATC
GAAATTCATCTAATCTTCCTCTTCCATTACCACCAAAGAAAACACGAATTATTTCTCATGTTTGGGAAAATAACAACCCTTTCCAAATTG
TCTTGGTTAAGGGAAATAAACTTAACACAGAGGAAACTGTAAAAGTCTTCTCCCCTGTCTGCCTCCCGGAGCTAGGACTGCAGAGGGGCC
TATCATGGTGCTTGCAGGCCCCCTGGCTGTCTCGCTGTTGCTGCCCAGCCTCACACTGCTGGTGTCCCACCTCTCCAGCTCCCAGGATGT
CTCCAGTGAGCCCAGCAGTGAGCAGCAGCTGTGCGCCCTTAGCAAGCACCCCACCGTGGCCTTTGAAGACCTGCAGCCGTGGGTCTCTAA
CTTCACCTACCCTGGAGCCCGGGATTTCTCCCAGCTGGCTTTGGACCCCTCCGGGAACCAGCTCATCGTGGGAGCCAGGAACTACCTCTT
CAGACTCAGCCTTGCCAATGTCTCTCTTCTTCAGGCCACAGAGTGGGCCTCCAGTGAGGACACGCGCCGCTCCTGCCAAAGCAAAGGGAA
GACTGAGGAGGAGTGTCAGAACTACGTGCGAGTCCTGATCGTCGCCGGCCGGAAGGTGTTCATGTGTGGAACCAATGCCTTTTCCCCCAT
GTGCACCAGCAGACAGGTGGGGAACCTCAGCCGGACTATTGAGAAGATCAATGGTGTGGCCCGCTGCCCCTATGACCCACGCCACAACTC
CACAGCTGTCATCTCCTCCCAGGGGGAGCTCTATGCAGCCACGGTCATCGACTTCTCAGGTCGGGACCCTGCCATCTACCGCAGCCTGGG
CAGTGGGCCACCGCTTCGCACTGCCCAATATAACTCCAAGTGGCTTAATGAGCCAAACTTCGTGGCAGCCTATGATATTGGGCTGTTTGC
ATACTTCTTCCTGCGGGAGAACGCAGTGGAGCACGACTGTGGACGCACCGTGTACTCTCGCGTGGCCCGCGTGTGCAAGAATGACGTGGG
GGGCCGATTCCTGCTGGAGGACACATGGACCACATTCATGAAGGCCCGGCTCAACTGCTCCCGCCCGGGCGAGGTCCCCTTCTACTATAA
CGAGCTGCAGAGTGCCTTCCACTTGCCGGAGCAGGACCTCATCTATGGAGTTTTCACAACCAACGTAAACAGCATCGCGGCTTCTGCTGT
CTGCGCCTTCAACCTCAGTGCTATCTCCCAGGCTTTCAATGGCCCATTTCGCTACCAGGAGAACCCCAGGGCTGCCTGGCTCCCCATAGC
CAACCCCATCCCCAATTTCCAGTGTGGCACCCTGCCTGAGACCGGTCCCAACGAGAACCTGACGGAGCGCAGCCTGCAGGACGCGCAGCG
CCTCTTCCTGATGAGCGAGGCCGTGCAGCCGGTGACACCCGAGCCCTGTGTCACCCAGGACAGCGTGCGCTTCTCACACCTCGTGGTGGA
CCTGGTGCAGGCTAAAGACACGCTCTACCATGTACTCTACATTGGCACCGAGTCGGGCACCATCCTGAAGGCGCTGTCCACGGCGAGCCG
CAGCCTCCACGGCTGCTACCTGGAGGAGCTGCACGTGCTGCCCCCCGGGCGCCGCGAGCCCCTGCGCAGCCTGCGCATCCTGCACAGCGC
CCGCGCGCTCTTCGTGGGGCTGAGAGACGGCGTCCTGCGGGTCCCACTGGAGAGGTGCGCCGCCTACCGCAGCCAGGGGGCATGCCTGGG
GGCCCGGGACCCGTACTGTGGCTGGGACGGGAAGCAGCAACGTTGCAGCACACTCGAGGACAGCTCCAACATGAGCCTCTGGACCCAGAA
CATCACCGCCTGTCCTGTGCGGAATGTGACACGGGATGGGGGCTTCGGCCCATGGTCACCATGGCAACCATGTGAGCACTTGGATGGGGA
CAACTCAGGCTCTTGCCTGTGTCGAGCTCGATCCTGTGATTCCCCTCGACCCCGCTGTGGGGGCCTTGACTGCCTGGGGCCAGCCATCCA
CATCGCCAACTGCTCCAGGAATGGGGCGTGGACCCCGTGGTCATCGTGGGCGCTGTGCAGCACGTCCTGTGGCATCGGCTTCCAGGTCCG
CCAGCGAAGTTGCAGCAACCCTGCTCCCCGCCACGGGGGCCGCATCTGCGTGGGCAAGAGCCGGGAGGAACGGTTCTGTAATGAGAACAC
GCCTTGCCCGGTGCCCATCTTCTGGGCTTCCTGGGGCTCCTGGAGCAAGTGCAGCAGCAACTGTGGAGGGGGCATGCAGTCGCGGCGTCG
GGCCTGCGAGAACGGCAACTCCTGCCTGGGCTGCGGCGTGGAGTTCAAGACGTGCAACCCCGAGGGCTGCCCCGAAGTGCGGCGCAACAC
CCCCTGGACGCCGTGGCTGCCCGTGAACGTGACGCAGGGCGGGGCACGGCAGGAGCAGCGGTTCCGCTTCACCTGCCGCGCGCCCCTTGC
AGACCCGCACGGCCTGCAGTTCGGCAGGAGAAGGACCGAGACGAGGACCTGTCCCGCGGACGGCTCCGGCTCCTGCGACACCGACGCCCT
GGTGGAGGTCCTCCTGCGCAGCGGGAGCACCTCCCCGCACACGGTGAGCGGGGGCTGGGCCGCCTGGGGCCCGTGGTCGTCCTGCTCCCG
GGACTGCGAGCTGGGCTTCCGCGTCCGCAAGAGAACGTGCACTAACCCGGAGCCCCGCAACGGGGGCCTGCCCTGCGTGGGCGATGCTGC
CGAGTACCAGGACTGCAACCCCCAGGCTTGCCCAGTTCGGGGTGCTTGGTCCTGCTGGACCTCATGGTCTCCATGCTCAGCTTCCTGTGG
TGGGGGTCACTATCAACGCACCCGTTCCTGCACCAGCCCCGCACCCTCCCCAGGTGAGGACATCTGTCTCGGGCTGCACACGGAGGAGGC
ACTATGTGCCACACAGGCCTGCCCAGAAGGCTGGTCGCCCTGGTCTGAGTGGAGTAAGTGCACTGACGACGGAGCCCAGAGCCGAAGCCG
GCACTGTGAGGAGCTCCTCCCAGGGTCCAGCGCCTGTGCTGGAAACAGCAGCCAGAGCCGCCCCTGCCCCTACAGCGAGATTCCCGTCAT
CCTGCCAGCCTCCAGCATGGAGGAGGCCACCGACTGTGCAGGGTTCAATCTCATCCACTTGGTGGCCACGGGCATCTCCTGCTTCTTGGG
CTCTGGGCTCCTGACCCTAGCAGTGTACCTGTCTTGCCAGCACTGCCAGCGTCAGTCCCAGGAGTCCACACTGGTCCATCCTGCCACCCC
CAACCATTTGCACTACAAGGGCGGAGGCACCCCGAAGAATGAAAAGTACACACCCATGGAATTCAAGACCCTGAACAAGAATAACTTGAT
CCCTGATGACAGAGCCAACTTCTACCCATTGCAGCAGACCAATGTGTACACGACTACTTACTACCCAAGCCCCCTGAACAAACACAGCTT
CCGGCCCGAGGCCTCACCTGGACAACGGTGCTTCCCCAACAGCTGATACCGCCGTCCTGGGGACTTGGGCTTCTTGCCTTCATAAGGCAC
AGAGCAGATGGAGATGGGACAGTGGAGCCAGTTTGGTTTTCTCCCTCTGCACTAGGCCAAGAACTTGCTGCCTTGCCTGTGGGGGGTCCC
ATCCGGCTTCAGAGAGCTCTGGCTGGCATTGACCATGGGGGAAAGGGCTGGTTTCAGGCTGACATATGGCCGCAGGTCCAGTTCAGCCCA
GGTCTCTCATGGTTATCTTCCAACCCACTGTCACGCTGACACTATGCTGCCATGCCTGGGCTGTGGACCTACTGGGCATTTGAGGAATTG
GAGAATGGAGATGGCAAGAGGGCAGGCTTTTAAGTTTGGGTTGGAGACAACTTCCTGTGGCCCCCACAAGCTGAGTCTGGCCTTCTCCAG
CTGGCCCCAAAAAAGGCCTTTGCTACATCCTGATTATCTCTGAAAGTAATCAATCAAGTGGCTCCAGTAGCTCTGGATTTTCTGCCAGGG
CTGGGCCATTGTGGTGCTGCCCCAGTATGACATGGGACCAAGGCCAGCGCAGGTTATCCACCTCTGCCTGGAAGTCTATACTCTACCCAG
GGCATCCCTCTGGTCAGAGGCAGTGAGTACTGGGAACTGGAGGCTGACCTGTGCTTAGAAGTCCTTTAATCTGGGCTGGTACAGGCCTCA
GCCTTGCCCTCAATGCACGAAAGGTGGCCCAGGAGAGAGGATCAATGCCATAGGAGGCAGAAGTCTGGCCTCTGTGCCTCTATGGAGACT
ATCTTCCAGTTGCTGCTCAACAGAGTTGTTGGCTGAGACCTGCTTGGGAGTCTCTGCTGGCCCTTCATCTGTTCAGGAACACACACACAC
ACACACTCACACACGCACACACAATCACAATTTGCTACAGCAACAAAAAAGACATTGGGCTGTGGCATTATTAATTAAAGATGATATCCA

>In-frame_ENST00000477593_ENST00000357599_TCGA-G7-6793_PIK3CB_chr3_138452202_-_SEMA5B_chr3_122667556_length(amino acids)=1105AA_start in transcript=1138_stop in transcript=4455
MPPGARTAEGPIMVLAGPLAVSLLLPSLTLLVSHLSSSQDVSSEPSSEQQLCALSKHPTVAFEDLQPWVSNFTYPGARDFSQLALDPSGN
QLIVGARNYLFRLSLANVSLLQATEWASSEDTRRSCQSKGKTEEECQNYVRVLIVAGRKVFMCGTNAFSPMCTSRQVGNLSRTIEKINGV
ARCPYDPRHNSTAVISSQGELYAATVIDFSGRDPAIYRSLGSGPPLRTAQYNSKWLNEPNFVAAYDIGLFAYFFLRENAVEHDCGRTVYS
RVARVCKNDVGGRFLLEDTWTTFMKARLNCSRPGEVPFYYNELQSAFHLPEQDLIYGVFTTNVNSIAASAVCAFNLSAISQAFNGPFRYQ
ENPRAAWLPIANPIPNFQCGTLPETGPNENLTERSLQDAQRLFLMSEAVQPVTPEPCVTQDSVRFSHLVVDLVQAKDTLYHVLYIGTESG
TILKALSTASRSLHGCYLEELHVLPPGRREPLRSLRILHSARALFVGLRDGVLRVPLERCAAYRSQGACLGARDPYCGWDGKQQRCSTLE
DSSNMSLWTQNITACPVRNVTRDGGFGPWSPWQPCEHLDGDNSGSCLCRARSCDSPRPRCGGLDCLGPAIHIANCSRNGAWTPWSSWALC
STSCGIGFQVRQRSCSNPAPRHGGRICVGKSREERFCNENTPCPVPIFWASWGSWSKCSSNCGGGMQSRRRACENGNSCLGCGVEFKTCN
PEGCPEVRRNTPWTPWLPVNVTQGGARQEQRFRFTCRAPLADPHGLQFGRRRTETRTCPADGSGSCDTDALVEVLLRSGSTSPHTVSGGW
AAWGPWSSCSRDCELGFRVRKRTCTNPEPRNGGLPCVGDAAEYQDCNPQACPVRGAWSCWTSWSPCSASCGGGHYQRTRSCTSPAPSPGE
DICLGLHTEEALCATQACPEGWSPWSEWSKCTDDGAQSRSRHCEELLPGSSACAGNSSQSRPCPYSEIPVILPASSMEEATDCAGFNLIH
LVATGISCFLGSGLLTLAVYLSCQHCQRQSQESTLVHPATPNHLHYKGGGTPKNEKYTPMEFKTLNKNNLIPDDRANFYPLQQTNVYTTT

--------------------------------------------------------------
>In-frame_ENST00000477593_ENST00000195173_TCGA-G7-6793_PIK3CB_chr3_138452202_-_SEMA5B_chr3_122667556_length(transcript)=5528nt_BP=1124nt
GGGCTGCTGACTGCGCACTTGGAATAGTAGCAGGCGGCGGCGGCGGAACGCCAGGCAGGGCCCCGTGGTTATGAATGTGCTTCAGTTTCA
TAATGCCTCCTGCTATGGCAGACATCCTTGACATCTGGGCGGTGGATTCACAGATAGCATCTGATGGCTCCATACCTGTGGATTTCCTTT
TGCCCACTGGGATTTATATCCAGTTGGAGGTACCTCGGGAAGCTACCATTTCTTATATTAAGCAGATGTTATGGAAGCAAGTTCACAATT
ACCCAATGTTCAACCTCCTTATGGATATTGACTCCTATATGTTTGCATGTGTGAATCAGACTGCTGTATATGAGGAGCTTGAAGATGAAA
CACGAAGACTCTGTGATGTCAGACCTTTTCTTCCAGTTCTCAAATTAGTGACAAGAAGTTGTGACCCAGGGGAAAAATTAGACTCAAAAA
TTGGAGTCCTTATAGGAAAAGGTCTGCATGAATTTGATTCCTTGAAGGATCCTGAAGTAAATGAATTTCGAAGAAAAATGCGCAAATTCA
GCGAGGAAAAAATCCTGTCACTTGTGGGATTGTCTTGGATGGACTGGCTAAAACAAACATATCCACCAGAGCATGAACCATCCATCCCTG
AAAACTTAGAAGATAAACTTTATGGGGGAAAGCTCATCGTAGCTGTTCATTTTGAAAACTGCCAGGACGTGTTTAGCTTTCAAGTGTCTC
CTAATATGAATCCTATCAAAGTAAATGAATTGGCAATCCAAAAACGTTTGACTATTCATGGGAAGGAAGATGAAGTTAGCCCCTATGATT
ATGTGTTGCAAGTCAGCGGGAGAGTAGAATATGTTTTTGGTGATCATCCACTAATTCAGTTCCAGTATATCCGGAACTGTGTGATGAACA
GAGCCCTGCCCCATTTTATACTTGTGGAATGCTGCAAGATCAAGAAAATGTATGAACAAGAAATGATTGCCATAGAGGCTGCCATAAATC
GAAATTCATCTAATCTTCCTCTTCCATTACCACCAAAGAAAACACGAATTATTTCTCATGTTTGGGAAAATAACAACCCTTTCCAAATTG
TCTTGGTTAAGGGAAATAAACTTAACACAGAGGAAACTGTAAAAGTCTTCTCCCCTGTCTGCCTCCCGGAGCTAGGACTGCAGAGGGGCC
TATCATGGTGCTTGCAGGCCCCCTGGCTGTCTCGCTGTTGCTGCCCAGCCTCACACTGCTGGTGTCCCACCTCTCCAGCTCCCAGGATGT
CTCCAGTGAGCCCAGCAGTGAGCAGCAGCTGTGCGCCCTTAGCAAGCACCCCACCGTGGCCTTTGAAGACCTGCAGCCGTGGGTCTCTAA
CTTCACCTACCCTGGAGCCCGGGATTTCTCCCAGCTGGCTTTGGACCCCTCCGGGAACCAGCTCATCGTGGGAGCCAGGAACTACCTCTT
CAGACTCAGCCTTGCCAATGTCTCTCTTCTTCAGGCCACAGAGTGGGCCTCCAGTGAGGACACGCGCCGCTCCTGCCAAAGCAAAGGGAA
GACTGAGGAGGAGTGTCAGAACTACGTGCGAGTCCTGATCGTCGCCGGCCGGAAGGTGTTCATGTGTGGAACCAATGCCTTTTCCCCCAT
GTGCACCAGCAGACAGGTGGGGAACCTCAGCCGGACTATTGAGAAGATCAATGGTGTGGCCCGCTGCCCCTATGACCCACGCCACAACTC
CACAGCTGTCATCTCCTCCCAGGGGGAGCTCTATGCAGCCACGGTCATCGACTTCTCAGGTCGGGACCCTGCCATCTACCGCAGCCTGGG
CAGTGGGCCACCGCTTCGCACTGCCCAATATAACTCCAAGTGGCTTAATGAGCCAAACTTCGTGGCAGCCTATGATATTGGGCTGTTTGC
ATACTTCTTCCTGCGGGAGAACGCAGTGGAGCACGACTGTGGACGCACCGTGTACTCTCGCGTGGCCCGCGTGTGCAAGAATGACGTGGG
GGGCCGATTCCTGCTGGAGGACACATGGACCACATTCATGAAGGCCCGGCTCAACTGCTCCCGCCCGGGCGAGGTCCCCTTCTACTATAA
CGAGCTGCAGAGTGCCTTCCACTTGCCGGAGCAGGACCTCATCTATGGAGTTTTCACAACCAACGTAAACAGCATCGCGGCTTCTGCTGT
CTGCGCCTTCAACCTCAGTGCTATCTCCCAGGCTTTCAATGGCCCATTTCGCTACCAGGAGAACCCCAGGGCTGCCTGGCTCCCCATAGC
CAACCCCATCCCCAATTTCCAGTGTGGCACCCTGCCTGAGACCGGTCCCAACGAGAACCTGACGGAGCGCAGCCTGCAGGACGCGCAGCG
CCTCTTCCTGATGAGCGAGGCCGTGCAGCCGGTGACACCCGAGCCCTGTGTCACCCAGGACAGCGTGCGCTTCTCACACCTCGTGGTGGA
CCTGGTGCAGGCTAAAGACACGCTCTACCATGTACTCTACATTGGCACCGAGTCGGGCACCATCCTGAAGGCGCTGTCCACGGCGAGCCG
CAGCCTCCACGGCTGCTACCTGGAGGAGCTGCACGTGCTGCCCCCCGGGCGCCGCGAGCCCCTGCGCAGCCTGCGCATCCTGCACAGCGC
CCGCGCGCTCTTCGTGGGGCTGAGAGACGGCGTCCTGCGGGTCCCACTGGAGAGGTGCGCCGCCTACCGCAGCCAGGGGGCATGCCTGGG
GGCCCGGGACCCGTACTGTGGCTGGGACGGGAAGCAGCAACGTTGCAGCACACTCGAGGACAGCTCCAACATGAGCCTCTGGACCCAGAA
CATCACCGCCTGTCCTGTGCGGAATGTGACACGGGATGGGGGCTTCGGCCCATGGTCACCATGGCAACCATGTGAGCACTTGGATGGGGA
CAACTCAGGCTCTTGCCTGTGTCGAGCTCGATCCTGTGATTCCCCTCGACCCCGCTGTGGGGGCCTTGACTGCCTGGGGCCAGCCATCCA
CATCGCCAACTGCTCCAGGAATGGGGCGTGGACCCCGTGGTCATCGTGGGCGCTGTGCAGCACGTCCTGTGGCATCGGCTTCCAGGTCCG
CCAGCGAAGTTGCAGCAACCCTGCTCCCCGCCACGGGGGCCGCATCTGCGTGGGCAAGAGCCGGGAGGAACGGTTCTGTAATGAGAACAC
GCCTTGCCCGGTGCCCATCTTCTGGGCTTCCTGGGGCTCCTGGAGCAAGTGCAGCAGCAACTGTGGAGGGGGCATGCAGTCGCGGCGTCG
GGCCTGCGAGAACGGCAACTCCTGCCTGGGCTGCGGCGAGTTCAAGACGTGCAACCCCGAGGGCTGCCCCGAAGTGCGGCGCAACACCCC
CTGGACGCCGTGGCTGCCCGTGAACGTGACGCAGGGCGGGGCACGGCAGGAGCAGCGGTTCCGCTTCACCTGCCGCGCGCCCCTTGCAGA
CCCGCACGGCCTGCAGTTCGGCAGGAGAAGGACCGAGACGAGGACCTGTCCCGCGGACGGCTCCGGCTCCTGCGACACCGACGCCCTGGT
GGAGGTCCTCCTGCGCAGCGGGAGCACCTCCCCGCACACGGTGAGCGGGGGCTGGGCCGCCTGGGGCCCGTGGTCGTCCTGCTCCCGGGA
CTGCGAGCTGGGCTTCCGCGTCCGCAAGAGAACGTGCACTAACCCGGAGCCCCGCAACGGGGGCCTGCCCTGCGTGGGCGATGCTGCCGA
GTACCAGGACTGCAACCCCCAGGCTTGCCCAGTTCGGGGTGCTTGGTCCTGCTGGACCTCATGGTCTCCATGCTCAGCTTCCTGTGGTGG
GGGTCACTATCAACGCACCCGTTCCTGCACCAGCCCCGCACCCTCCCCAGGTGAGGACATCTGTCTCGGGCTGCACACGGAGGAGGCACT
ATGTGCCACACAGGCCTGCCCAGGCTGGTCGCCCTGGTCTGAGTGGAGTAAGTGCACTGACGACGGAGCCCAGAGCCGAAGCCGGCACTG
TGAGGAGCTCCTCCCAGGGTCCAGCGCCTGTGCTGGAAACAGCAGCCAGAGCCGCCCCTGCCCCTACAGCGAGATTCCCGTCATCCTGCC
AGCCTCCAGCATGGAGGAGGCCACCGACTGTGCAGGTAAAAGAAACCGGACCTACCTCATGCTGCGGTCCTCCCAGCCCTCCGGCACCCC
ACTCCAAAGTCTGGACTCTTTCCACATCCTGCTCCAGACAGCCAAGCTTTGTTGGGGTCCCCACTGCTTTGAGATGGGTTCAATCTCATC
CACTTGGTGGCCACGGGCATCTCCTGCTTCTTGGGCTCTGGGCTCCTGACCCTAGCAGTGTACCTGTCTTGCCAGCACTGCCAGCGTCAG
TCCCAGGAGTCCACACTGGTCCATCCTGCCACCCCCAACCATTTGCACTACAAGGGCGGAGGCACCCCGAAGAATGAAAAGTACACACCC
ATGGAATTCAAGACCCTGAACAAGAATAACTTGATCCCTGATGACAGAGCCAACTTCTACCCATTGCAGCAGACCAATGTGTACACGACT
ACTTACTACCCAAGCCCCCTGAACAAACACAGCTTCCGGCCCGAGGCCTCACCTGGACAACGGTGCTTCCCCAACAGCTGATACCGCCGT
CCTGGGGACTTGGGCTTCTTGCCTTCATAAGGCACAGAGCAGATGGAGATGGGACAGTGGAGCCAGTTTGGTTTTCTCCCTCTGCACTAG
GCCAAGAACTTGCTGCCTTGCCTGTGGGGGGTCCCATCCGGCTTCAGAGAGCTCTGGCTGGCATTGACCATGGGGGAAAGGGCTGGTTTC
AGGCTGACATATGGCCGCAGGTCCAGTTCAGCCCAGGTCTCTCATGGTTATCTTCCAACCCACTGTCACGCTGACACTATGCTGCCATGC
CTGGGCTGTGGACCTACTGGGCATTTGAGGAATTGGAGAATGGAGATGGCAAGAGGGCAGGCTTTTAAGTTTGGGTTGGAGACAACTTCC
TGTGGCCCCCACAAGCTGAGTCTGGCCTTCTCCAGCTGGCCCCAAAAAAGGCCTTTGCTACATCCTGATTATCTCTGAAAGTAATCAATC
AAGTGGCTCCAGTAGCTCTGGATTTTCTGCCAGGGCTGGGCCATTGTGGTGCTGCCCCAGTATGACATGGGACCAAGGCCAGCGCAGGTT
ATCCACCTCTGCCTGGAAGTCTATACTCTACCCAGGGCATCCCTCTGGTCAGAGGCAGTGAGTACTGGGAACTGGAGGCTGACCTGTGCT
TAGAAGTCCTTTAATCTGGGCTGGTACAGGCCTCAGCCTTGCCCTCAATGCACGAAAGGTGGCCCAGGAGAGAGGATCAATGCCATAGGA
GGCAGAAGTCTGGCCTCTGTGCCTCTATGGAGACTATCTTCCAGTTGCTGCTCAACAGAGTTGTTGGCTGAGACCTGCTTGGGAGTCTCT
GCTGGCCCTTCATCTGTTCAGGAACACACACACACACACACTCACACACGCACACACAATCACAATTTGCTACAGCAACAAAAAAGACAT

>In-frame_ENST00000477593_ENST00000195173_TCGA-G7-6793_PIK3CB_chr3_138452202_-_SEMA5B_chr3_122667556_length(amino acids)=1046AA_start in transcript=1138_stop in transcript=4278
MPPGARTAEGPIMVLAGPLAVSLLLPSLTLLVSHLSSSQDVSSEPSSEQQLCALSKHPTVAFEDLQPWVSNFTYPGARDFSQLALDPSGN
QLIVGARNYLFRLSLANVSLLQATEWASSEDTRRSCQSKGKTEEECQNYVRVLIVAGRKVFMCGTNAFSPMCTSRQVGNLSRTIEKINGV
ARCPYDPRHNSTAVISSQGELYAATVIDFSGRDPAIYRSLGSGPPLRTAQYNSKWLNEPNFVAAYDIGLFAYFFLRENAVEHDCGRTVYS
RVARVCKNDVGGRFLLEDTWTTFMKARLNCSRPGEVPFYYNELQSAFHLPEQDLIYGVFTTNVNSIAASAVCAFNLSAISQAFNGPFRYQ
ENPRAAWLPIANPIPNFQCGTLPETGPNENLTERSLQDAQRLFLMSEAVQPVTPEPCVTQDSVRFSHLVVDLVQAKDTLYHVLYIGTESG
TILKALSTASRSLHGCYLEELHVLPPGRREPLRSLRILHSARALFVGLRDGVLRVPLERCAAYRSQGACLGARDPYCGWDGKQQRCSTLE
DSSNMSLWTQNITACPVRNVTRDGGFGPWSPWQPCEHLDGDNSGSCLCRARSCDSPRPRCGGLDCLGPAIHIANCSRNGAWTPWSSWALC
STSCGIGFQVRQRSCSNPAPRHGGRICVGKSREERFCNENTPCPVPIFWASWGSWSKCSSNCGGGMQSRRRACENGNSCLGCGEFKTCNP
EGCPEVRRNTPWTPWLPVNVTQGGARQEQRFRFTCRAPLADPHGLQFGRRRTETRTCPADGSGSCDTDALVEVLLRSGSTSPHTVSGGWA
AWGPWSSCSRDCELGFRVRKRTCTNPEPRNGGLPCVGDAAEYQDCNPQACPVRGAWSCWTSWSPCSASCGGGHYQRTRSCTSPAPSPGED
ICLGLHTEEALCATQACPGWSPWSEWSKCTDDGAQSRSRHCEELLPGSSACAGNSSQSRPCPYSEIPVILPASSMEEATDCAGKRNRTYL

--------------------------------------------------------------
>In-frame_ENST00000477593_ENST00000451055_TCGA-G7-6793_PIK3CB_chr3_138452202_-_SEMA5B_chr3_122667556_length(transcript)=4935nt_BP=1124nt
GGGCTGCTGACTGCGCACTTGGAATAGTAGCAGGCGGCGGCGGCGGAACGCCAGGCAGGGCCCCGTGGTTATGAATGTGCTTCAGTTTCA
TAATGCCTCCTGCTATGGCAGACATCCTTGACATCTGGGCGGTGGATTCACAGATAGCATCTGATGGCTCCATACCTGTGGATTTCCTTT
TGCCCACTGGGATTTATATCCAGTTGGAGGTACCTCGGGAAGCTACCATTTCTTATATTAAGCAGATGTTATGGAAGCAAGTTCACAATT
ACCCAATGTTCAACCTCCTTATGGATATTGACTCCTATATGTTTGCATGTGTGAATCAGACTGCTGTATATGAGGAGCTTGAAGATGAAA
CACGAAGACTCTGTGATGTCAGACCTTTTCTTCCAGTTCTCAAATTAGTGACAAGAAGTTGTGACCCAGGGGAAAAATTAGACTCAAAAA
TTGGAGTCCTTATAGGAAAAGGTCTGCATGAATTTGATTCCTTGAAGGATCCTGAAGTAAATGAATTTCGAAGAAAAATGCGCAAATTCA
GCGAGGAAAAAATCCTGTCACTTGTGGGATTGTCTTGGATGGACTGGCTAAAACAAACATATCCACCAGAGCATGAACCATCCATCCCTG
AAAACTTAGAAGATAAACTTTATGGGGGAAAGCTCATCGTAGCTGTTCATTTTGAAAACTGCCAGGACGTGTTTAGCTTTCAAGTGTCTC
CTAATATGAATCCTATCAAAGTAAATGAATTGGCAATCCAAAAACGTTTGACTATTCATGGGAAGGAAGATGAAGTTAGCCCCTATGATT
ATGTGTTGCAAGTCAGCGGGAGAGTAGAATATGTTTTTGGTGATCATCCACTAATTCAGTTCCAGTATATCCGGAACTGTGTGATGAACA
GAGCCCTGCCCCATTTTATACTTGTGGAATGCTGCAAGATCAAGAAAATGTATGAACAAGAAATGATTGCCATAGAGGCTGCCATAAATC
GAAATTCATCTAATCTTCCTCTTCCATTACCACCAAAGAAAACACGAATTATTTCTCATGTTTGGGAAAATAACAACCCTTTCCAAATTG
TCTTGGTTAAGGGAAATAAACTTAACACAGAGGAAACTGTAAAAGTCTTCTCCCCTGTCTGCCTCCCGGAGCTAGGACTGCAGAGGGGCC
TATCATGGTGCTTGCAGGCCCCCTGGCTGTCTCGCTGTTGCTGCCCAGCCTCACACTGCTGGTGTCCCACCTCTCCAGCTCCCAGGATGT
CTCCAGTGAGCCCAGCAGTGAGCAGCAGCTGTGCGCCCTTAGCAAGCACCCCACCGTGGCCTTTGAAGACCTGCAGCCGTGGGTCTCTAA
CTTCACCTACCCTGGAGCCCGGGATTTCTCCCAGCTGGCTTTGGACCCCTCCGGGAACCAGCTCATCGTGGGAGCCAGGAACTACCTCTT
CAGACTCAGCCTTGCCAATGTCTCTCTTCTTCAGGCCACAGAGTGGGCCTCCAGTGAGGACACGCGCCGCTCCTGCCAAAGCAAAGGGAA
GACTGAGGAGGAGTGTCAGAACTACGTGCGAGTCCTGATCGTCGCCGGCCGGAAGGTGTTCATGTGTGGAACCAATGCCTTTTCCCCCAT
GTGCACCAGCAGACAGGTGGGGAACCTCAGCCGGACTATTGAGAAGATCAATGGTGTGGCCCGCTGCCCCTATGACCCACGCCACAACTC
CACAGCTGTCATCTCCTCCCAGGGGGAGCTCTATGCAGCCACGGTCATCGACTTCTCAGGTCGGGACCCTGCCATCTACCGCAGCCTGGG
CAGTGGGCCACCGCTTCGCACTGCCCAATATAACTCCAAGTGGCTTAATGAGCCAAACTTCGTGGCAGCCTATGATATTGGGCTGTTTGC
ATACTTCTTCCTGCGGGAGAACGCAGTGGAGCACGACTGTGGACGCACCGTGTACTCTCGCGTGGCCCGCGTGTGCAAGAATGACGTGGG
GGGCCGATTCCTGCTGGAGGACACATGGACCACATTCATGAAGGCCCGGCTCAACTGCTCCCGCCCGGGCGAGGTCCCCTTCTACTATAA
CGAGCTGCAGAGTGCCTTCCACTTGCCGGAGCAGGACCTCATCTATGGAGTTTTCACAACCAACGTAAACAGCATCGCGGCTTCTGCTGT
CTGCGCCTTCAACCTCAGTGCTATCTCCCAGGCTTTCAATGGCCCATTTCGCTACCAGGAGAACCCCAGGGCTGCCTGGCTCCCCATAGC
CAACCCCATCCCCAATTTCCAGTGTGGCACCCTGCCTGAGACCGGTCCCAACGAGAACCTGACGGAGCGCAGCCTGCAGGACGCGCAGCG
CCTCTTCCTGATGAGCGAGGCCGTGCAGCCGGTGACACCCGAGCCCTGTGTCACCCAGGACAGCGTGCGCTTCTCACACCTCGTGGTGGA
CCTGGTGCAGGCTAAAGACACGCTCTACCATGTACTCTACATTGGCACCGAGTCGGGCACCATCCTGAAGGCGCTGTCCACGGCGAGCCG
CAGCCTCCACGGCTGCTACCTGGAGGAGCTGCACGTGCTGCCCCCCGGGCGCCGCGAGCCCCTGCGCAGCCTGCGCATCCTGCACAGCGC
CCGCGCGCTCTTCGTGGGGCTGAGAGACGGCGTCCTGCGGGTCCCACTGGAGAGGTGCGCCGCCTACCGCAGCCAGGGGGCATGCCTGGG
GGCCCGGGACCCGTACTGTGGCTGGGACGGGAAGCAGCAACGTTGCAGCACACTCGAGGACAGCTCCAACATGAGCCTCTGGACCCAGAA
CATCACCGCCTGTCCTGTGCGGAATGTGACACGGGATGGGGGCTTCGGCCCATGGTCACCATGGCAACCATGTGAGCACTTGGATGGGGA
CAACTCAGGCTCTTGCCTGTGTCGAGCTCGATCCTGTGATTCCCCTCGACCCCGCTGTGGGGGCCTTGACTGCCTGGGGCCAGCCATCCA
CATCGCCAACTGCTCCAGGAATGGGGCGTGGACCCCGTGGTCATCGTGGGCGCTGTGCAGCACGTCCTGTGGCATCGGCTTCCAGGTCCG
CCAGCGAAGTTGCAGCAACCCTGCTCCCCGCCACGGGGGCCGCATCTGCGTGGGCAAGAGCCGGGAGGAACGGTTCTGTAATGAGAACAC
GCCTTGCCCGGTGCCCATCTTCTGGGCTTCCTGGGGCTCCTGGAGCAAGTGCAGCAGCAACTGTGGAGGGGGCATGCAGTCGCGGCGTCG
GGCCTGCGAGAACGGCAACTCCTGCCTGGGCTGCGGCGTGGAGTTCAAGACGTGCAACCCCGAGGGCTGCCCCGAAGTGCGGCGCAACAC
CCCCTGGACGCCGTGGCTGCCCGTGAACGTGACGCAGGGCGGGGCACGGCAGGAGCAGCGGTTCCGCTTCACCTGCCGCGCGCCCCTTGC
AGACCCGCACGGCCTGCAGTTCGGCAGGAGAAGGACCGAGACGAGGACCTGTCCCGCGGACGGCTCCGGCTCCTGCGACACCGACGCCCT
GGTGGAGGTCCTCCTGCGCAGCGGGAGCACCTCCCCGCACACGGTGAGCGGGGGCTGGGCCGCCTGGGGCCCGTGGTCGTCCTGCTCCCG
GGACTGCGAGCTGGGCTTCCGCGTCCGCAAGAGAACGTGCACTAACCCGGAGCCCCGCAACGGGGGCCTGCCCTGCGTGGGCGATGCTGC
CGAGTACCAGGACTGCAACCCCCAGGCTTGCCCAGTTCGGGGTGCTTGGTCCTGCTGGACCTCATGGTCTCCATGCTCAGCTTCCTGTGG
TGGGGGTCACTATCAACGCACCCGTTCCTGCACCAGCCCCGCACCCTCCCCAGGTGAGGACATCTGTCTCGGGCTGCACACGGAGGAGGC
ACTATGTGCCACACAGGCCTGCCCAGAAGGCTGGTCGCCCTGGTCTGAGTGGAGTAAGTGCACTGACGACGGAGCCCAGAGCCGAAGCCG
GCACTGTGAGGAGCTCCTCCCAGGGTCCAGCGCCTGTGCTGGAAACAGCAGCCAGAGCCGCCCCTGCCCCTACAGCGAGATTCCCGTCAT
CCTGCCAGCCTCCAGCATGGAGGAGGCCACCGACTGTGCAGGGTTCAATCTCATCCACTTGGTGGCCACGGGCATCTCCTGCTTCTTGGG
CTCTGGGCTCCTGACCCTAGCAGTGTACCTGTCTTGCCAGCACTGCCAGCGTCAGTCCCAGGAGTCCACACTGGTCCATCCTGCCACCCC
CAACCATTTGCACTACAAGGGCGGAGGCACCCCGAAGAATGAAAAGTACACACCCATGGAATTCAAGACCCTGAACAAGAATAACTTGAT
CCCTGATGACAGAGCCAACTTCTACCCATTGCAGCAGACCAATGTGTACACGACTACTTACTACCCAAGCCCCCTGAACAAACACAGCTT
CCGGCCCGAGGCCTCACCTGGACAACGGTGCTTCCCCAACAGCTGATACCGCCGTCCTGGGGACTTGGGCTTCTTGCCTTCATAAGGCAC
AGAGCAGATGGAGATGGGACAGTGGAGCCAGTTTGGTTTTCTCCCTCTGCACTAGGCCAAGAACTTGCTGCCTTGCCTGTGGGGGGTCCC
ATCCGGCTTCAGAGAGCTCTGGCTGGCATTGACCATGGGGGAAAGGGCTGGTTTCAGGCTGACATATGGCCGCAGGTCCAGTTCAGCCCA
GGTCTCTCATGGTTATCTTCCAACCCACTGTCACGCTGACACTATGCTGCCATGCCTGGGCTGTGGACCTACTGGGCATTTGAGGAATTG
GAGAATGGAGATGGCAAGAGGGCAGGCTTTTAAGTTTGGGTTGGAGACAACTTCCTGTGGCCCCCACAAGCTGAGTCTGGCCTTCTCCAG

>In-frame_ENST00000477593_ENST00000451055_TCGA-G7-6793_PIK3CB_chr3_138452202_-_SEMA5B_chr3_122667556_length(amino acids)=1105AA_start in transcript=1138_stop in transcript=4455
MPPGARTAEGPIMVLAGPLAVSLLLPSLTLLVSHLSSSQDVSSEPSSEQQLCALSKHPTVAFEDLQPWVSNFTYPGARDFSQLALDPSGN
QLIVGARNYLFRLSLANVSLLQATEWASSEDTRRSCQSKGKTEEECQNYVRVLIVAGRKVFMCGTNAFSPMCTSRQVGNLSRTIEKINGV
ARCPYDPRHNSTAVISSQGELYAATVIDFSGRDPAIYRSLGSGPPLRTAQYNSKWLNEPNFVAAYDIGLFAYFFLRENAVEHDCGRTVYS
RVARVCKNDVGGRFLLEDTWTTFMKARLNCSRPGEVPFYYNELQSAFHLPEQDLIYGVFTTNVNSIAASAVCAFNLSAISQAFNGPFRYQ
ENPRAAWLPIANPIPNFQCGTLPETGPNENLTERSLQDAQRLFLMSEAVQPVTPEPCVTQDSVRFSHLVVDLVQAKDTLYHVLYIGTESG
TILKALSTASRSLHGCYLEELHVLPPGRREPLRSLRILHSARALFVGLRDGVLRVPLERCAAYRSQGACLGARDPYCGWDGKQQRCSTLE
DSSNMSLWTQNITACPVRNVTRDGGFGPWSPWQPCEHLDGDNSGSCLCRARSCDSPRPRCGGLDCLGPAIHIANCSRNGAWTPWSSWALC
STSCGIGFQVRQRSCSNPAPRHGGRICVGKSREERFCNENTPCPVPIFWASWGSWSKCSSNCGGGMQSRRRACENGNSCLGCGVEFKTCN
PEGCPEVRRNTPWTPWLPVNVTQGGARQEQRFRFTCRAPLADPHGLQFGRRRTETRTCPADGSGSCDTDALVEVLLRSGSTSPHTVSGGW
AAWGPWSSCSRDCELGFRVRKRTCTNPEPRNGGLPCVGDAAEYQDCNPQACPVRGAWSCWTSWSPCSASCGGGHYQRTRSCTSPAPSPGE
DICLGLHTEEALCATQACPEGWSPWSEWSKCTDDGAQSRSRHCEELLPGSSACAGNSSQSRPCPYSEIPVILPASSMEEATDCAGFNLIH
LVATGISCFLGSGLLTLAVYLSCQHCQRQSQESTLVHPATPNHLHYKGGGTPKNEKYTPMEFKTLNKNNLIPDDRANFYPLQQTNVYTTT

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Fusion Gene PPI Analysis for PIK3CB-SEMA5B


check button Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in

ChiPPI page.


check button Protein-protein interactors with each fusion partner protein in wild-type (BIOGRID-3.4.160)
HgeneHgene's interactorsTgeneTgene's interactors


check button - Retained PPIs in in-frame fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenStill interaction with


check button - Lost PPIs in in-frame fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenInteraction lost with


check button - Retained PPIs, but lost function due to frame-shift fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenInteraction lost with


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Related Drugs for PIK3CB-SEMA5B


check button Drugs targeting genes involved in this fusion gene.
(DrugBank Version 5.1.8 2021-05-08)
PartnerGeneUniProtAccDrugBank IDDrug nameDrug activityDrug typeDrug status

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Related Diseases for PIK3CB-SEMA5B


check button Diseases associated with fusion partners.
(DisGeNet 4.0)
PartnerGeneDisease IDDisease name# pubmedsSource
HgenePIK3CBC0033578Prostatic Neoplasms1CTD_human
HgenePIK3CBC0036341Schizophrenia1CTD_human
HgenePIK3CBC0079744Diffuse Large B-Cell Lymphoma1CTD_human
HgenePIK3CBC0376358Malignant neoplasm of prostate1CTD_human