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Center for Computational Systems Medicine
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Fusion Gene Summary

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Fusion Gene ORF analysis

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Fusion Genomic Features

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Fusion Protein Features

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Fusion Gene Sequence

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Fusion Gene PPI analysis

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Related Drugs

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Related Diseases

Fusion gene:PUM1-DDX52 (FusionGDB2 ID:70221)

Fusion Gene Summary for PUM1-DDX52

check button Fusion gene summary
Fusion gene informationFusion gene name: PUM1-DDX52
Fusion gene ID: 70221
HgeneTgene
Gene symbol

PUM1

DDX52

Gene ID

9698

11056

Gene namepumilio RNA binding family member 1DExD-box helicase 52
SynonymsHSPUM|PUMH|PUMH1|PUML1|SCA47HUSSY19|ROK1
Cytomap

1p35.2

17q12

Type of geneprotein-codingprotein-coding
Descriptionpumilio homolog 1pumilio-1probable ATP-dependent RNA helicase DDX52DEAD (Asp-Glu-Ala-Asp) box polypeptide 52DEAD box protein 52DEAD-box helicase 52
Modification date2020031320200313
UniProtAcc.

Q9Y2R4

Ensembl transtripts involved in fusion geneENST00000424085, ENST00000257075, 
ENST00000373747, ENST00000426105, 
ENST00000440538, ENST00000373741, 
ENST00000423018, ENST00000373742, 
ENST00000490546, 		ENST00000349699, 
ENST00000394367, 
Fusion gene scores* DoF score38 X 31 X 15=176705 X 5 X 5=125
# samples 566
** MAII scorelog2(56/17670*10)=-4.97973140249401
possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs).
DoF>8 and MAII<0		log2(6/125*10)=-1.05889368905357
possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs).
DoF>8 and MAII<0
Context

PubMed: PUM1 [Title/Abstract] AND DDX52 [Title/Abstract] AND fusion [Title/Abstract]

Most frequent breakpointPUM1(31532051)-DDX52(35974398), # samples:1
Anticipated loss of major functional domain due to fusion event.PUM1-DDX52 seems lost the major protein functional domain in Hgene partner, which is a essential gene due to the frame-shifted ORF.
PUM1-DDX52 seems lost the major protein functional domain in Tgene partner, which is a essential gene due to the frame-shifted ORF.
* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

check button Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez
PartnerGeneGO IDGO termPubMed ID
HgenePUM1

GO:0010608

posttranscriptional regulation of gene expression

25100735

HgenePUM1

GO:0043488

regulation of mRNA stability

26724866

HgenePUM1

GO:0051726

regulation of cell cycle

20818387

HgenePUM1

GO:0051983

regulation of chromosome segregation

26724866

HgenePUM1

GO:1900246

positive regulation of RIG-I signaling pathway

25340845

HgenePUM1

GO:2000637

positive regulation of gene silencing by miRNA

20818387|22345517


check buttonFusion gene breakpoints across PUM1 (5'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

check buttonFusion gene breakpoints across DDX52 (3'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

check button Fusion gene information from two resources (ChiTars 5.0 and ChimerDB 4.0)
* All genome coordinats were lifted-over on hg19.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.
SourceDiseaseSampleHgeneHchrHbpHstrandTgeneTchrTbpTstrand
ChimerDB4STADTCGA-BR-8284-01APUM1chr1

31532051

-DDX52chr17

35974398

-


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Fusion Gene ORF analysis for PUM1-DDX52

check button Open reading frame (ORF) analsis of fusion genes based on Ensembl gene isoform structure.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.
ORFHenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrand
In-frameENST00000424085ENST00000349699PUM1chr1

31532051

-DDX52chr17

35974398

-
In-frameENST00000424085ENST00000394367PUM1chr1

31532051

-DDX52chr17

35974398

-
In-frameENST00000257075ENST00000349699PUM1chr1

31532051

-DDX52chr17

35974398

-
In-frameENST00000257075ENST00000394367PUM1chr1

31532051

-DDX52chr17

35974398

-
In-frameENST00000373747ENST00000349699PUM1chr1

31532051

-DDX52chr17

35974398

-
In-frameENST00000373747ENST00000394367PUM1chr1

31532051

-DDX52chr17

35974398

-
In-frameENST00000426105ENST00000349699PUM1chr1

31532051

-DDX52chr17

35974398

-
In-frameENST00000426105ENST00000394367PUM1chr1

31532051

-DDX52chr17

35974398

-
In-frameENST00000440538ENST00000349699PUM1chr1

31532051

-DDX52chr17

35974398

-
In-frameENST00000440538ENST00000394367PUM1chr1

31532051

-DDX52chr17

35974398

-
Frame-shiftENST00000373741ENST00000349699PUM1chr1

31532051

-DDX52chr17

35974398

-
Frame-shiftENST00000373741ENST00000394367PUM1chr1

31532051

-DDX52chr17

35974398

-
In-frameENST00000423018ENST00000349699PUM1chr1

31532051

-DDX52chr17

35974398

-
In-frameENST00000423018ENST00000394367PUM1chr1

31532051

-DDX52chr17

35974398

-
Frame-shiftENST00000373742ENST00000349699PUM1chr1

31532051

-DDX52chr17

35974398

-
Frame-shiftENST00000373742ENST00000394367PUM1chr1

31532051

-DDX52chr17

35974398

-
intron-3CDSENST00000490546ENST00000349699PUM1chr1

31532051

-DDX52chr17

35974398

-
intron-3CDSENST00000490546ENST00000394367PUM1chr1

31532051

-DDX52chr17

35974398

-

check buttonORFfinder result based on the fusion transcript sequence of in-frame fusion genes.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandSeq length
(transcript)		BP loci
(transcript)		Predicted start
(transcript)		Predicted stop
(transcript)		Seq length
(amino acids)
ENST00000424085PUM1chr131532051-ENST00000349699DDX52chr1735974398-502745491498135
ENST00000424085PUM1chr131532051-ENST00000394367DDX52chr1735974398-244045491498135
ENST00000257075PUM1chr131532051-ENST00000349699DDX52chr1735974398-503045794501135
ENST00000257075PUM1chr131532051-ENST00000394367DDX52chr1735974398-244345794501135
ENST00000373747PUM1chr131532051-ENST00000349699DDX52chr1735974398-5036463100507135
ENST00000373747PUM1chr131532051-ENST00000394367DDX52chr1735974398-2449463100507135
ENST00000426105PUM1chr131532051-ENST00000349699DDX52chr1735974398-502745491498135
ENST00000426105PUM1chr131532051-ENST00000394367DDX52chr1735974398-244045491498135
ENST00000440538PUM1chr131532051-ENST00000349699DDX52chr1735974398-501644380487135
ENST00000440538PUM1chr131532051-ENST00000394367DDX52chr1735974398-242944380487135
ENST00000423018PUM1chr131532051-ENST00000349699DDX52chr1735974398-502545289496135
ENST00000423018PUM1chr131532051-ENST00000394367DDX52chr1735974398-243845289496135

check buttonDeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandNo-coding scoreCoding score
ENST00000424085ENST00000349699PUM1chr131532051-DDX52chr1735974398-0.0774168150.9225832
ENST00000424085ENST00000394367PUM1chr131532051-DDX52chr1735974398-0.0706331060.92936695
ENST00000257075ENST00000349699PUM1chr131532051-DDX52chr1735974398-0.077885590.92211443
ENST00000257075ENST00000394367PUM1chr131532051-DDX52chr1735974398-0.0890385660.9109614
ENST00000373747ENST00000349699PUM1chr131532051-DDX52chr1735974398-0.077374520.9226255
ENST00000373747ENST00000394367PUM1chr131532051-DDX52chr1735974398-0.103216910.8967831
ENST00000426105ENST00000349699PUM1chr131532051-DDX52chr1735974398-0.0774168150.9225832
ENST00000426105ENST00000394367PUM1chr131532051-DDX52chr1735974398-0.0706331060.92936695
ENST00000440538ENST00000349699PUM1chr131532051-DDX52chr1735974398-0.0765330050.923467
ENST00000440538ENST00000394367PUM1chr131532051-DDX52chr1735974398-0.0536796640.9463203
ENST00000423018ENST00000349699PUM1chr131532051-DDX52chr1735974398-0.0772115440.9227885
ENST00000423018ENST00000394367PUM1chr131532051-DDX52chr1735974398-0.1077653840.8922346

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Fusion Genomic Features for PUM1-DDX52


check buttonFusionAI prediction of the potential fusion gene breakpoint based on the pre-mature RNA sequence context (+/- 5kb of individual partner genes, total 20kb length sequence). FusionAI is a fusion gene breakpoint classifier based on convolutional neural network by comparing the fusion positive and negative sequence context of ~ 20K fusion gene data. From here, we can have the relative potentency of the 20K genomic sequence how individual sequnce will be likely used as the gene fusion breakpoints.
HgeneHchrHbpHstrandTgeneTchrTbpTstrand1-pp (fusion gene breakpoint)

check buttonDistribution of 44 human genomic features loci across 20kb length fusion breakpoint regions. We integrated a total of 44 different types of human genomic feature loci information across five big categories including virus integration sites, repeats, structural variants, chromatin states, and gene expression regulation. More details are in help page.

check buttonDistribution of 44 human genomic features loci across 20kb length fusion breakpoint regions that are ovelapped with the top 1% feature importance score regions. More details are in help page.

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Fusion Protein Features for PUM1-DDX52


check button Go to

FGviewer for the breakpoints of chr1:31532051-chr17:35974398

.
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
FGviewer

check buttonMain function of each fusion partner protein. (from UniProt)
HgeneTgene
.DDX52

Q9Y2R4

FUNCTION: Might normally function as a transcriptional repressor. EWS-fusion-proteins (EFPS) may play a role in the tumorigenic process. They may disturb gene expression by mimicking, or interfering with the normal function of CTD-POLII within the transcription initiation complex. They may also contribute to an aberrant activation of the fusion protein target genes.

check buttonRetention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at

download page


* Minus value of BPloci means that the break pointn is located before the CDS.
- In-frame and retained protein feature among the 13 regional features.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note

- In-frame and not-retained protein feature among the 13 regional features.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note
HgenePUM1chr1:31532051chr17:35974398ENST00000257075-222393_613121.01187.0Compositional biasNote=Ala-rich
HgenePUM1chr1:31532051chr17:35974398ENST00000257075-222475_523121.01187.0Compositional biasNote=Gln-rich
HgenePUM1chr1:31532051chr17:35974398ENST00000257075-222642_815121.01187.0Compositional biasNote=Ser-rich
HgenePUM1chr1:31532051chr17:35974398ENST00000426105-222393_613121.01189.0Compositional biasNote=Ala-rich
HgenePUM1chr1:31532051chr17:35974398ENST00000426105-222475_523121.01189.0Compositional biasNote=Gln-rich
HgenePUM1chr1:31532051chr17:35974398ENST00000426105-222642_815121.01189.0Compositional biasNote=Ser-rich
HgenePUM1chr1:31532051chr17:35974398ENST00000440538-222393_613121.01163.0Compositional biasNote=Ala-rich
HgenePUM1chr1:31532051chr17:35974398ENST00000440538-222475_523121.01163.0Compositional biasNote=Gln-rich
HgenePUM1chr1:31532051chr17:35974398ENST00000440538-222642_815121.01163.0Compositional biasNote=Ser-rich
HgenePUM1chr1:31532051chr17:35974398ENST00000257075-222828_1168121.01187.0DomainPUM-HD
HgenePUM1chr1:31532051chr17:35974398ENST00000426105-222828_1168121.01189.0DomainPUM-HD
HgenePUM1chr1:31532051chr17:35974398ENST00000440538-222828_1168121.01163.0DomainPUM-HD
HgenePUM1chr1:31532051chr17:35974398ENST00000257075-2221028_1063121.01187.0RepeatPumilio 6
HgenePUM1chr1:31532051chr17:35974398ENST00000257075-2221064_1099121.01187.0RepeatPumilio 7
HgenePUM1chr1:31532051chr17:35974398ENST00000257075-2221103_1142121.01187.0RepeatPumilio 8
HgenePUM1chr1:31532051chr17:35974398ENST00000257075-222848_883121.01187.0RepeatPumilio 1
HgenePUM1chr1:31532051chr17:35974398ENST00000257075-222884_919121.01187.0RepeatPumilio 2
HgenePUM1chr1:31532051chr17:35974398ENST00000257075-222920_955121.01187.0RepeatPumilio 3
HgenePUM1chr1:31532051chr17:35974398ENST00000257075-222956_991121.01187.0RepeatPumilio 4
HgenePUM1chr1:31532051chr17:35974398ENST00000257075-222992_1027121.01187.0RepeatPumilio 5
HgenePUM1chr1:31532051chr17:35974398ENST00000426105-2221028_1063121.01189.0RepeatPumilio 6
HgenePUM1chr1:31532051chr17:35974398ENST00000426105-2221064_1099121.01189.0RepeatPumilio 7
HgenePUM1chr1:31532051chr17:35974398ENST00000426105-2221103_1142121.01189.0RepeatPumilio 8
HgenePUM1chr1:31532051chr17:35974398ENST00000426105-222848_883121.01189.0RepeatPumilio 1
HgenePUM1chr1:31532051chr17:35974398ENST00000426105-222884_919121.01189.0RepeatPumilio 2
HgenePUM1chr1:31532051chr17:35974398ENST00000426105-222920_955121.01189.0RepeatPumilio 3
HgenePUM1chr1:31532051chr17:35974398ENST00000426105-222956_991121.01189.0RepeatPumilio 4
HgenePUM1chr1:31532051chr17:35974398ENST00000426105-222992_1027121.01189.0RepeatPumilio 5
HgenePUM1chr1:31532051chr17:35974398ENST00000440538-2221028_1063121.01163.0RepeatPumilio 6
HgenePUM1chr1:31532051chr17:35974398ENST00000440538-2221064_1099121.01163.0RepeatPumilio 7
HgenePUM1chr1:31532051chr17:35974398ENST00000440538-2221103_1142121.01163.0RepeatPumilio 8
HgenePUM1chr1:31532051chr17:35974398ENST00000440538-222848_883121.01163.0RepeatPumilio 1
HgenePUM1chr1:31532051chr17:35974398ENST00000440538-222884_919121.01163.0RepeatPumilio 2
HgenePUM1chr1:31532051chr17:35974398ENST00000440538-222920_955121.01163.0RepeatPumilio 3
HgenePUM1chr1:31532051chr17:35974398ENST00000440538-222956_991121.01163.0RepeatPumilio 4
HgenePUM1chr1:31532051chr17:35974398ENST00000440538-222992_1027121.01163.0RepeatPumilio 5
TgeneDDX52chr1:31532051chr17:35974398ENST000003496991315543_592580.6666666666666600.0Compositional biasNote=Lys-rich
TgeneDDX52chr1:31532051chr17:35974398ENST000003496991315196_374580.6666666666666600.0DomainHelicase ATP-binding
TgeneDDX52chr1:31532051chr17:35974398ENST000003496991315385_546580.6666666666666600.0DomainHelicase C-terminal
TgeneDDX52chr1:31532051chr17:35974398ENST000003496991315165_193580.6666666666666600.0MotifNote=Q motif
TgeneDDX52chr1:31532051chr17:35974398ENST000003496991315318_321580.6666666666666600.0MotifNote=DEAD box
TgeneDDX52chr1:31532051chr17:35974398ENST000003496991315209_216580.6666666666666600.0Nucleotide bindingNote=ATP


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Fusion Gene Sequence for PUM1-DDX52


check button For in-frame fusion transcripts, we provide the fusion transcript sequences and fusion amino acid sequences. To have fusion amino acid sequence, we ran ORFfinder and chose the longest ORF among the all predicted ones.
>In-frame_ENST00000424085_ENST00000349699_TCGA-BR-8284-01A_PUM1_chr1_31532051_-_DDX52_chr17_35974398_length(transcript)=5027nt_BP=454nt
GTGAAAGGTAAGGGGGAGCGAGAGCGCCAGAGAGAGAAGATCGGGGGGCTGAAATCCATCTTCATCCTACCGCTCCGCCCGTGTTGGTGG
AATGAGCGTTGCATGTGTCTTGAAGAGAAAAGCAGTGCTTTGGCAGGACTCTTTCAGCCCCCACCTGAAACATCACCCTCAAGAACCAGC
TAATCCCAACATGCCTGTTGTTTTGACATCTGGAACAGGGTCGCAAGCGCAGCCACAACCAGCTGCAAATCAGGCTCTTGCAGCTGGGAC
TCACTCCAGCCCTGTCCCAGGATCTATAGGAGTTGCAGGCCGTTCCCAGGACGACGCTATGGTGGACTACTTCTTTCAGAGGCAGCATGG
TGAGCAGCTTGGGGGAGGAGGAAGTGGAGGAGGCGGCTATAATAATAGCAAACATCGATGGCCTACTGGGGATAACATTCATGCAGAACA
TCAGGAAAAAGGTCACTGGTCAGAACAGCAAGAAGAAAGTAGCTCTTGAAGACAAAAGTTAAAAACAGACTTTAAAAATACTGTCCCAGA
AATGTAATTTTATGATCCCAGCATGAATGTTATTTTCATGGAATACTTGAAGTCTTACAGTCACCTGTACCAAACATTTGAAATCAACTA
CAAGTACATGGGACTGGTGATAAATGATCCTAAACTATCAAGTCAGTTTCAATTTGTAGGTGCCTTTTTTTTTTCCTGTAGAGATGAGGG
TCTTGCCATGTTGTCCAGGCTGGTCTTGAACTCCTGACCTCACACAATCCTCCTGCCTTAGCCTCCTGAGTAACTGAGATTACAGGCACA
AGCTGCTGCACCCAGCTCTGTAGGTGACTTTTAAATGATTATACAATGGAAATAACATTCATTGACATTTCTGTGGTTTGAATCCAGAGA
GATACTTCTTATAGAAAAACAAATGTTTATGCTAAAAATAACACCAAAATGTGGTGAACTCTTAAGGACTTTTCCCTTCAAGTGTGAAGG
AAGGTGTGATGAATGCTGTGGAGAGGCATCTGGAACAGAAATTCAAAATAAAGCCTTGACATTAAATACCCCTTCCACTGCTCACTTTGT
GGATGGTAGCATGAGCTGTCTACCAAGAAGAAACCTGCTGCTCTCTTAATTTTAATATTTCCTAATTTGTTGATGGCCTTTTGTGTTGTG
AACCACAACAAAGAGAGGCCTCTTTTGTGGCTGGTTATTCCAGTTCCCTGGGATTTTAAATTCTTTGGTCTATTAAGTATCCTTGTATTG
GATACGTAATACCTTAGTGCTGTCATAATGTTGTACAAGATCATGATCAGCTTCTCCCTTTCTTCATTTTCTGTGATTTAACCATGTTCT
TTCCTGTCTCTTTCCATTTAAGATATTTTATTTGAATACTGATAAACATTTTATCCTGATAAGGAAGAATGTTCTTGTTACTTGATATAC
CTCTGTCTTCATTCTCTTACAGCTTATCTTTCCTTAGGTTGATGGTGCCTCATTTAATAAGTAGATCTCTACTTAAGCTAAAGGATTATG
ATCATATGGTGTTGGAGACTATTAGCTATTAGATTTTTCTCAAAATATGAGTTTTGTACAATGGAATGAAAAAGTGACTTCATATACGTA
AGACTGGTGCCTAAGAATGACTTGAATGTTATCAGTACTACCACAGAACTATAATATACATTGCCTTTTCTCAGCAGCATTTGAATTCAA
CTTATTTATTTCCTCTATTTGTAGCTTTTTGTTCTAGAGTCAGAACAGGTGGACTTTGCTTTGAATATTTTAGATGGGACAAAAATTGTT
CATCTTCGGAATTTATTTAAAGAAAGATCTACTGGTACAGGGTGTAAAAATACTTACAAGAATATGATTTTTTTGTTTGTTCATGCAAAC
AGATCCTTTACCCAATATATATTCTGGAGGTAGGTATACTGGTGCAACCCATTTGCCAGCTGTGTGACTTTGAGTAAGTTCTTAATTTCT
CAGAGTTTCAGTGTTCTCATCTATAAAATTATGTCTCCAATTGTGAGTACCTAGCACTGTACCTGATACATAGTAAACTCACAATAAATA
TTTCTTAAACATAGATTTTGTTAGGAATACAGGCTTGATAACACAGTCCTTACCCTTACAATGTAATGTTAGAGATAAAGGAGCAAATAG
AGTCACGCATCACTTAATGACAGAGATATGTTCTGAGAAATGCATCGTTAGGTGATATTGTCATTGTGTAAACATCATGTTGTTTACTTC
CACAAACCTAGATGGTATAGCCTACCAGTACACCTAGGCTATATGGTATAGTCTGTTGCTCCTAGGCTACAAACCCGTATAGCATGTTAC
TGTCCTGCATGCTATAGACAGTTGGAACATGTAGACTTGTATAAACACTGCACACTTAACCTATACTAAATTTATTGAAAATATTTTTCT
TGGCCAGGCGTAGTGGCTCATGCCTGTAATCCTAACACTTCGGGAGGCCTAGGCGGGCAGGTTGTCTGAGCTCAGGAGTTTAGAGACCAG
CCCGGGCAACATGGCAAAACCCCATCTCTACTAAAAAAAATACCAAAAAAAGTTAGCCGGGTGTGGTGGCATAGTCCCGGCTACTCAGGA
GTCTGAGGCAGGAGAATAGCTTGAACCTGTGAGGCGGAGGTTGCAGTAAGCCGAGATCGCGCTACTGCACTCCAGCCTGGGCGACAGGGC
AAGACTGTCTTGAAAATAAACACACACACACACATTATATATATTATGTATTATATATATTAGATATATATTTTTTCTTCAATAAATTAA
CCTTAGCTTACTGTAACTTTTACTTTATGAGTTTTTTTGTTTTGTTTTGTTTTTTTTTTTTGAGAAACAGGGTCTGTTACCTAATTTGGA
GTTTAGTGGCACAATCATGGCCCACTGCAGCCTTGACCTCTTAGACTCAAGTGATCCTCAAACCTCAGCCTCCCGAGTAGCTGGTACTAT
AGGTGCGGGCCACCAACACTCAGCTAATTTTTAAATTTTGTAGAGACGAGGTCTTGCTTGTTGTCCAGTTGGGTTTTTTAGACTTTTTTT
TTTTTTTTTTTTTGGAGATGGAGTCTTGCTCTGTTGCCCAGGCTGGAGTGCAGTGGCATGATCTCGGCTCACTGCAACCTCTGACTCCTG
GGTTCAAGTGATTCTCCTGCCTCAGCCTCCCGAGTTGCTGGGACTACAGGCATGCGCCACCACACCCAGCTAATTTTTGTATTTTTAGTG
ACAGGGTTTCACCATGTTGGCCAGGCTGGTCTCAAACTCCTGACCTGGAGTGAGCCACTATATCTGGCCTTTATAAACTTTTTTGATTCT
TGTCATAACACTTAGCCTAAAATGCAAATGTACAGCTGTAGAAAAATACTTTATTTCTTTATATCCTTATTCTAGAAGCTTTTTTTCTAT
TAATTTTTGTTTGTTTGTTTTGTTTTACTATTTACTTCTAAAACTTTTTTGTTAAAAACCATGGCACAAACACACACATTATGCTAGGCA
TACAAAAGGTCAGGATCATCAGTCTCACTGTCTTCCACCCCCACTTCTCATCCCACCATTGTATCTGCTGTCTGTGGCTGACCAAAACAT
CATCATGTAGCACATGACTAGTGTGGCAAGTGCTCTGTTAGATGTAAGGCCATGATGCTAAAGCATCACAAGAGGGCATCTAACCCAGAT
TGGGGATGTCATGGAAGGCCGACATCCTGAGTTGAATCCTGCAAATGTAAAAACCAATAGGCAAAGAAGAGGAACAAAAAGGATTCCAGG
ACAAACTGAGGTCACATCTATGATCCTTGACTTTATTGTGTCTGTTTAAAGTATCTACAGTAACCTGTATCAACTTAGTCAGTGTATTAA
TACTAAATTTAGCTCCTTCAAAGCAGTTGGAACTATGTGCTACATAAATTTCAGCTTCACACAAGGAAGGGAAGGAGTGAAATTAGTGAA
CAGGCAGTTACAGCAAAAGAAAAAACATAAAAATTGAATAGCTGGCTCTGGTGAAATGAGCAAGGACTTTAGAGTCAAACTGGCCTGGAT
TTGAATCCTGATCCTCATTGCTTGTAGCTGTATGATCTGGACAAATGACAGTAGCTGTTTCTAACCTTGATTTTCTCATCTGTAAGATGC
CAATTGTAACTCCTAAGGATACTGAGGATTTTTTAAAATGCGTGTACAGTTCCTGACCAGTGGTTTGTGCCTAATAACTTATTACAAATT
ATTACCCAGTAAAAACCTTGAGACAAGAGTGAAAACGTAAAGCTAATTAATCCATTACTTGTTAGCAAGCAAACTACGTGCTTGAGAAAA
TTACTCAACTTTCATGTTTTACTTCCAGACAGTAGTTTGATTAAAAGAAAAAAAAAAATCACAGCCCAGGCATGTGGCTCACACCCTGGC
ACTTGGAGGCCGAGGTGGGAGCATAGCTTGAGCCTAGCAGTTTGAAACTAGCCTGGGCAACATAGATCCTATCTCTACAAAAAAAAAAAA
AAAAAAAAAAAAATTGGCCCGGTGTGGTGGTGCATGCCTGCGGTCCCAGCTACTCAGGAGGCTGAGGCGAGAGGATCGCTTGAGCCAGGG
AGGCTGAGGCTGCTGTGAGCCATGATCATGCCACTGCACTCCAGCCTGGACAACAGAGCAAGACCCTGTCTCAAAAAAAAAAAAAAATCA
GTAAAAAATATGCCGAAATAGCTTCAGAGTAATTATGAATTGCCTTCCAAAAACCACTGTTTTGCACTTGCCTGCAAGAATAGGAAAAAC
AAAAGGACTGTAAGGTGGCAGGATGGCAGAACCAAACAAGAGTAGTACACCTTGGAGTTTTTTCGAAATATGGGTTGGGTTTTTGGGCTC
TTGGTTGATTTAAAATAAAATTTAAATGCCTTCAGCGTCTGTGATAGGTGCCAACATGAAGCTACCGAGTTCCAGAGAAAGAGGGGAGAG

>In-frame_ENST00000424085_ENST00000349699_TCGA-BR-8284-01A_PUM1_chr1_31532051_-_DDX52_chr17_35974398_length(amino acids)=135AA_start in transcript=91_stop in transcript=498
MSVACVLKRKAVLWQDSFSPHLKHHPQEPANPNMPVVLTSGTGSQAQPQPAANQALAAGTHSSPVPGSIGVAGRSQDDAMVDYFFQRQHG

--------------------------------------------------------------
>In-frame_ENST00000424085_ENST00000394367_TCGA-BR-8284-01A_PUM1_chr1_31532051_-_DDX52_chr17_35974398_length(transcript)=2440nt_BP=454nt
GTGAAAGGTAAGGGGGAGCGAGAGCGCCAGAGAGAGAAGATCGGGGGGCTGAAATCCATCTTCATCCTACCGCTCCGCCCGTGTTGGTGG
AATGAGCGTTGCATGTGTCTTGAAGAGAAAAGCAGTGCTTTGGCAGGACTCTTTCAGCCCCCACCTGAAACATCACCCTCAAGAACCAGC
TAATCCCAACATGCCTGTTGTTTTGACATCTGGAACAGGGTCGCAAGCGCAGCCACAACCAGCTGCAAATCAGGCTCTTGCAGCTGGGAC
TCACTCCAGCCCTGTCCCAGGATCTATAGGAGTTGCAGGCCGTTCCCAGGACGACGCTATGGTGGACTACTTCTTTCAGAGGCAGCATGG
TGAGCAGCTTGGGGGAGGAGGAAGTGGAGGAGGCGGCTATAATAATAGCAAACATCGATGGCCTACTGGGGATAACATTCATGCAGAACA
TCAGGAAAAAGGTCACTGGTCAGAACAGCAAGAAGAAAGTAGCTCTTGAAGACAAAAGTTAAAAACAGACTTTAAAAATACTGTCCCAGA
AATGTAATTTTATGATCCCAGCATGAATGTTATTTTCATGGAATACTTGAAGTCTTACAGTCACCTGTACCAAACATTTGAAATCAACTA
CAAGTACATGGGACTGGTGATAAATGATCCTAAACTATCAAGTCAGTTTCAATTTGTAGGTGCCTTTTTTTTTTCCTGTAGAGATGAGGG
TCTTGCCATGTTGTCCAGGCTGGTCTTGAACTCCTGACCTCACACAATCCTCCTGCCTTAGCCTCCTGAGTAACTGAGATTACAGGCACA
AGCTGCTGCACCCAGCTCTGTAGGTGACTTTTAAATGATTATACAATGGAAATAACATTCATTGACATTTCTGTGGTTTGAATCCAGAGA
GATACTTCTTATAGAAAAACAAATGTTTATGCTAAAAATAACACCAAAATGTGGTGAACTCTTAAGGACTTTTCCCTTCAAGTGTGAAGG
AAGGTGTGATGAATGCTGTGGAGAGGCATCTGGAACAGAAATTCAAAATAAAGCCTTGACATTAAATACCCCTTCCACTGCTCACTTTGT
GGATGGTAGCATGAGCTGTCTACCAAGAAGAAACCTGCTGCTCTCTTAATTTTAATATTTCCTAATTTGTTGATGGCCTTTTGTGTTGTG
AACCACAACAAAGAGAGGCCTCTTTTGTGGCTGGTTATTCCAGTTCCCTGGGATTTTAAATTCTTTGGTCTATTAAGTATCCTTGTATTG
GATACGTAATACCTTAGTGCTGTCATAATGTTGTACAAGATCATGATCAGCTTCTCCCTTTCTTCATTTTCTGTGATTTAACCATGTTCT
TTCCTGTCTCTTTCCATTTAAGATATTTTATTTGAATACTGATAAACATTTTATCCTGATAAGGAAGAATGTTCTTGTTACTTGATATAC
CTCTGTCTTCATTCTCTTACAGCTTATCTTTCCTTAGGTTGATGGTGCCTCATTTAATAAGTAGATCTCTACTTAAGCTAAAGGATTATG
ATCATATGGTGTTGGAGACTATTAGCTATTAGATTTTTCTCAAAATATGAGTTTTGTACAATGGAATGAAAAAGTGACTTCATATACGTA
AGACTGGTGCCTAAGAATGACTTGAATGTTATCAGTACTACCACAGAACTATAATATACATTGCCTTTTCTCAGCAGCATTTGAATTCAA
CTTATTTATTTCCTCTATTTGTAGCTTTTTGTTCTAGAGTCAGAACAGGTGGACTTTGCTTTGAATATTTTAGATGGGACAAAAATTGTT
CATCTTCGGAATTTATTTAAAGAAAGATCTACTGGTACAGGGTGTAAAAATACTTACAAGAATATGATTTTTTTGTTTGTTCATGCAAAC
AGATCCTTTACCCAATATATATTCTGGAGGTAGGTATACTGGTGCAACCCATTTGCCAGCTGTGTGACTTTGAGTAAGTTCTTAATTTCT
CAGAGTTTCAGTGTTCTCATCTATAAAATTATGTCTCCAATTGTGAGTACCTAGCACTGTACCTGATACATAGTAAACTCACAATAAATA
TTTCTTAAACATAGATTTTGTTAGGAATACAGGCTTGATAACACAGTCCTTACCCTTACAATGTAATGTTAGAGATAAAGGAGCAAATAG
AGTCACGCATCACTTAATGACAGAGATATGTTCTGAGAAATGCATCGTTAGGTGATATTGTCATTGTGTAAACATCATGTTGTTTACTTC
CACAAACCTAGATGGTATAGCCTACCAGTACACCTAGGCTATATGGTATAGTCTGTTGCTCCTAGGCTACAAACCCGTATAGCATGTTAC
TGTCCTGCATGCTATAGACAGTTGGAACATGTAGACTTGTATAAACACTGCACACTTAACCTATACTAAATTTATTGAAAATATTTTTCT

>In-frame_ENST00000424085_ENST00000394367_TCGA-BR-8284-01A_PUM1_chr1_31532051_-_DDX52_chr17_35974398_length(amino acids)=135AA_start in transcript=91_stop in transcript=498
MSVACVLKRKAVLWQDSFSPHLKHHPQEPANPNMPVVLTSGTGSQAQPQPAANQALAAGTHSSPVPGSIGVAGRSQDDAMVDYFFQRQHG

--------------------------------------------------------------
>In-frame_ENST00000257075_ENST00000349699_TCGA-BR-8284-01A_PUM1_chr1_31532051_-_DDX52_chr17_35974398_length(transcript)=5030nt_BP=457nt
GGAGTGAAAGGTAAGGGGGAGCGAGAGCGCCAGAGAGAGAAGATCGGGGGGCTGAAATCCATCTTCATCCTACCGCTCCGCCCGTGTTGG
TGGAATGAGCGTTGCATGTGTCTTGAAGAGAAAAGCAGTGCTTTGGCAGGACTCTTTCAGCCCCCACCTGAAACATCACCCTCAAGAACC
AGCTAATCCCAACATGCCTGTTGTTTTGACATCTGGAACAGGGTCGCAAGCGCAGCCACAACCAGCTGCAAATCAGGCTCTTGCAGCTGG
GACTCACTCCAGCCCTGTCCCAGGATCTATAGGAGTTGCAGGCCGTTCCCAGGACGACGCTATGGTGGACTACTTCTTTCAGAGGCAGCA
TGGTGAGCAGCTTGGGGGAGGAGGAAGTGGAGGAGGCGGCTATAATAATAGCAAACATCGATGGCCTACTGGGGATAACATTCATGCAGA
ACATCAGGAAAAAGGTCACTGGTCAGAACAGCAAGAAGAAAGTAGCTCTTGAAGACAAAAGTTAAAAACAGACTTTAAAAATACTGTCCC
AGAAATGTAATTTTATGATCCCAGCATGAATGTTATTTTCATGGAATACTTGAAGTCTTACAGTCACCTGTACCAAACATTTGAAATCAA
CTACAAGTACATGGGACTGGTGATAAATGATCCTAAACTATCAAGTCAGTTTCAATTTGTAGGTGCCTTTTTTTTTTCCTGTAGAGATGA
GGGTCTTGCCATGTTGTCCAGGCTGGTCTTGAACTCCTGACCTCACACAATCCTCCTGCCTTAGCCTCCTGAGTAACTGAGATTACAGGC
ACAAGCTGCTGCACCCAGCTCTGTAGGTGACTTTTAAATGATTATACAATGGAAATAACATTCATTGACATTTCTGTGGTTTGAATCCAG
AGAGATACTTCTTATAGAAAAACAAATGTTTATGCTAAAAATAACACCAAAATGTGGTGAACTCTTAAGGACTTTTCCCTTCAAGTGTGA
AGGAAGGTGTGATGAATGCTGTGGAGAGGCATCTGGAACAGAAATTCAAAATAAAGCCTTGACATTAAATACCCCTTCCACTGCTCACTT
TGTGGATGGTAGCATGAGCTGTCTACCAAGAAGAAACCTGCTGCTCTCTTAATTTTAATATTTCCTAATTTGTTGATGGCCTTTTGTGTT
GTGAACCACAACAAAGAGAGGCCTCTTTTGTGGCTGGTTATTCCAGTTCCCTGGGATTTTAAATTCTTTGGTCTATTAAGTATCCTTGTA
TTGGATACGTAATACCTTAGTGCTGTCATAATGTTGTACAAGATCATGATCAGCTTCTCCCTTTCTTCATTTTCTGTGATTTAACCATGT
TCTTTCCTGTCTCTTTCCATTTAAGATATTTTATTTGAATACTGATAAACATTTTATCCTGATAAGGAAGAATGTTCTTGTTACTTGATA
TACCTCTGTCTTCATTCTCTTACAGCTTATCTTTCCTTAGGTTGATGGTGCCTCATTTAATAAGTAGATCTCTACTTAAGCTAAAGGATT
ATGATCATATGGTGTTGGAGACTATTAGCTATTAGATTTTTCTCAAAATATGAGTTTTGTACAATGGAATGAAAAAGTGACTTCATATAC
GTAAGACTGGTGCCTAAGAATGACTTGAATGTTATCAGTACTACCACAGAACTATAATATACATTGCCTTTTCTCAGCAGCATTTGAATT
CAACTTATTTATTTCCTCTATTTGTAGCTTTTTGTTCTAGAGTCAGAACAGGTGGACTTTGCTTTGAATATTTTAGATGGGACAAAAATT
GTTCATCTTCGGAATTTATTTAAAGAAAGATCTACTGGTACAGGGTGTAAAAATACTTACAAGAATATGATTTTTTTGTTTGTTCATGCA
AACAGATCCTTTACCCAATATATATTCTGGAGGTAGGTATACTGGTGCAACCCATTTGCCAGCTGTGTGACTTTGAGTAAGTTCTTAATT
TCTCAGAGTTTCAGTGTTCTCATCTATAAAATTATGTCTCCAATTGTGAGTACCTAGCACTGTACCTGATACATAGTAAACTCACAATAA
ATATTTCTTAAACATAGATTTTGTTAGGAATACAGGCTTGATAACACAGTCCTTACCCTTACAATGTAATGTTAGAGATAAAGGAGCAAA
TAGAGTCACGCATCACTTAATGACAGAGATATGTTCTGAGAAATGCATCGTTAGGTGATATTGTCATTGTGTAAACATCATGTTGTTTAC
TTCCACAAACCTAGATGGTATAGCCTACCAGTACACCTAGGCTATATGGTATAGTCTGTTGCTCCTAGGCTACAAACCCGTATAGCATGT
TACTGTCCTGCATGCTATAGACAGTTGGAACATGTAGACTTGTATAAACACTGCACACTTAACCTATACTAAATTTATTGAAAATATTTT
TCTTGGCCAGGCGTAGTGGCTCATGCCTGTAATCCTAACACTTCGGGAGGCCTAGGCGGGCAGGTTGTCTGAGCTCAGGAGTTTAGAGAC
CAGCCCGGGCAACATGGCAAAACCCCATCTCTACTAAAAAAAATACCAAAAAAAGTTAGCCGGGTGTGGTGGCATAGTCCCGGCTACTCA
GGAGTCTGAGGCAGGAGAATAGCTTGAACCTGTGAGGCGGAGGTTGCAGTAAGCCGAGATCGCGCTACTGCACTCCAGCCTGGGCGACAG
GGCAAGACTGTCTTGAAAATAAACACACACACACACATTATATATATTATGTATTATATATATTAGATATATATTTTTTCTTCAATAAAT
TAACCTTAGCTTACTGTAACTTTTACTTTATGAGTTTTTTTGTTTTGTTTTGTTTTTTTTTTTTGAGAAACAGGGTCTGTTACCTAATTT
GGAGTTTAGTGGCACAATCATGGCCCACTGCAGCCTTGACCTCTTAGACTCAAGTGATCCTCAAACCTCAGCCTCCCGAGTAGCTGGTAC
TATAGGTGCGGGCCACCAACACTCAGCTAATTTTTAAATTTTGTAGAGACGAGGTCTTGCTTGTTGTCCAGTTGGGTTTTTTAGACTTTT
TTTTTTTTTTTTTTTTGGAGATGGAGTCTTGCTCTGTTGCCCAGGCTGGAGTGCAGTGGCATGATCTCGGCTCACTGCAACCTCTGACTC
CTGGGTTCAAGTGATTCTCCTGCCTCAGCCTCCCGAGTTGCTGGGACTACAGGCATGCGCCACCACACCCAGCTAATTTTTGTATTTTTA
GTGACAGGGTTTCACCATGTTGGCCAGGCTGGTCTCAAACTCCTGACCTGGAGTGAGCCACTATATCTGGCCTTTATAAACTTTTTTGAT
TCTTGTCATAACACTTAGCCTAAAATGCAAATGTACAGCTGTAGAAAAATACTTTATTTCTTTATATCCTTATTCTAGAAGCTTTTTTTC
TATTAATTTTTGTTTGTTTGTTTTGTTTTACTATTTACTTCTAAAACTTTTTTGTTAAAAACCATGGCACAAACACACACATTATGCTAG
GCATACAAAAGGTCAGGATCATCAGTCTCACTGTCTTCCACCCCCACTTCTCATCCCACCATTGTATCTGCTGTCTGTGGCTGACCAAAA
CATCATCATGTAGCACATGACTAGTGTGGCAAGTGCTCTGTTAGATGTAAGGCCATGATGCTAAAGCATCACAAGAGGGCATCTAACCCA
GATTGGGGATGTCATGGAAGGCCGACATCCTGAGTTGAATCCTGCAAATGTAAAAACCAATAGGCAAAGAAGAGGAACAAAAAGGATTCC
AGGACAAACTGAGGTCACATCTATGATCCTTGACTTTATTGTGTCTGTTTAAAGTATCTACAGTAACCTGTATCAACTTAGTCAGTGTAT
TAATACTAAATTTAGCTCCTTCAAAGCAGTTGGAACTATGTGCTACATAAATTTCAGCTTCACACAAGGAAGGGAAGGAGTGAAATTAGT
GAACAGGCAGTTACAGCAAAAGAAAAAACATAAAAATTGAATAGCTGGCTCTGGTGAAATGAGCAAGGACTTTAGAGTCAAACTGGCCTG
GATTTGAATCCTGATCCTCATTGCTTGTAGCTGTATGATCTGGACAAATGACAGTAGCTGTTTCTAACCTTGATTTTCTCATCTGTAAGA
TGCCAATTGTAACTCCTAAGGATACTGAGGATTTTTTAAAATGCGTGTACAGTTCCTGACCAGTGGTTTGTGCCTAATAACTTATTACAA
ATTATTACCCAGTAAAAACCTTGAGACAAGAGTGAAAACGTAAAGCTAATTAATCCATTACTTGTTAGCAAGCAAACTACGTGCTTGAGA
AAATTACTCAACTTTCATGTTTTACTTCCAGACAGTAGTTTGATTAAAAGAAAAAAAAAAATCACAGCCCAGGCATGTGGCTCACACCCT
GGCACTTGGAGGCCGAGGTGGGAGCATAGCTTGAGCCTAGCAGTTTGAAACTAGCCTGGGCAACATAGATCCTATCTCTACAAAAAAAAA
AAAAAAAAAAAAAAAATTGGCCCGGTGTGGTGGTGCATGCCTGCGGTCCCAGCTACTCAGGAGGCTGAGGCGAGAGGATCGCTTGAGCCA
GGGAGGCTGAGGCTGCTGTGAGCCATGATCATGCCACTGCACTCCAGCCTGGACAACAGAGCAAGACCCTGTCTCAAAAAAAAAAAAAAA
TCAGTAAAAAATATGCCGAAATAGCTTCAGAGTAATTATGAATTGCCTTCCAAAAACCACTGTTTTGCACTTGCCTGCAAGAATAGGAAA
AACAAAAGGACTGTAAGGTGGCAGGATGGCAGAACCAAACAAGAGTAGTACACCTTGGAGTTTTTTCGAAATATGGGTTGGGTTTTTGGG
CTCTTGGTTGATTTAAAATAAAATTTAAATGCCTTCAGCGTCTGTGATAGGTGCCAACATGAAGCTACCGAGTTCCAGAGAAAGAGGGGA

>In-frame_ENST00000257075_ENST00000349699_TCGA-BR-8284-01A_PUM1_chr1_31532051_-_DDX52_chr17_35974398_length(amino acids)=135AA_start in transcript=94_stop in transcript=501
MSVACVLKRKAVLWQDSFSPHLKHHPQEPANPNMPVVLTSGTGSQAQPQPAANQALAAGTHSSPVPGSIGVAGRSQDDAMVDYFFQRQHG

--------------------------------------------------------------
>In-frame_ENST00000257075_ENST00000394367_TCGA-BR-8284-01A_PUM1_chr1_31532051_-_DDX52_chr17_35974398_length(transcript)=2443nt_BP=457nt
GGAGTGAAAGGTAAGGGGGAGCGAGAGCGCCAGAGAGAGAAGATCGGGGGGCTGAAATCCATCTTCATCCTACCGCTCCGCCCGTGTTGG
TGGAATGAGCGTTGCATGTGTCTTGAAGAGAAAAGCAGTGCTTTGGCAGGACTCTTTCAGCCCCCACCTGAAACATCACCCTCAAGAACC
AGCTAATCCCAACATGCCTGTTGTTTTGACATCTGGAACAGGGTCGCAAGCGCAGCCACAACCAGCTGCAAATCAGGCTCTTGCAGCTGG
GACTCACTCCAGCCCTGTCCCAGGATCTATAGGAGTTGCAGGCCGTTCCCAGGACGACGCTATGGTGGACTACTTCTTTCAGAGGCAGCA
TGGTGAGCAGCTTGGGGGAGGAGGAAGTGGAGGAGGCGGCTATAATAATAGCAAACATCGATGGCCTACTGGGGATAACATTCATGCAGA
ACATCAGGAAAAAGGTCACTGGTCAGAACAGCAAGAAGAAAGTAGCTCTTGAAGACAAAAGTTAAAAACAGACTTTAAAAATACTGTCCC
AGAAATGTAATTTTATGATCCCAGCATGAATGTTATTTTCATGGAATACTTGAAGTCTTACAGTCACCTGTACCAAACATTTGAAATCAA
CTACAAGTACATGGGACTGGTGATAAATGATCCTAAACTATCAAGTCAGTTTCAATTTGTAGGTGCCTTTTTTTTTTCCTGTAGAGATGA
GGGTCTTGCCATGTTGTCCAGGCTGGTCTTGAACTCCTGACCTCACACAATCCTCCTGCCTTAGCCTCCTGAGTAACTGAGATTACAGGC
ACAAGCTGCTGCACCCAGCTCTGTAGGTGACTTTTAAATGATTATACAATGGAAATAACATTCATTGACATTTCTGTGGTTTGAATCCAG
AGAGATACTTCTTATAGAAAAACAAATGTTTATGCTAAAAATAACACCAAAATGTGGTGAACTCTTAAGGACTTTTCCCTTCAAGTGTGA
AGGAAGGTGTGATGAATGCTGTGGAGAGGCATCTGGAACAGAAATTCAAAATAAAGCCTTGACATTAAATACCCCTTCCACTGCTCACTT
TGTGGATGGTAGCATGAGCTGTCTACCAAGAAGAAACCTGCTGCTCTCTTAATTTTAATATTTCCTAATTTGTTGATGGCCTTTTGTGTT
GTGAACCACAACAAAGAGAGGCCTCTTTTGTGGCTGGTTATTCCAGTTCCCTGGGATTTTAAATTCTTTGGTCTATTAAGTATCCTTGTA
TTGGATACGTAATACCTTAGTGCTGTCATAATGTTGTACAAGATCATGATCAGCTTCTCCCTTTCTTCATTTTCTGTGATTTAACCATGT
TCTTTCCTGTCTCTTTCCATTTAAGATATTTTATTTGAATACTGATAAACATTTTATCCTGATAAGGAAGAATGTTCTTGTTACTTGATA
TACCTCTGTCTTCATTCTCTTACAGCTTATCTTTCCTTAGGTTGATGGTGCCTCATTTAATAAGTAGATCTCTACTTAAGCTAAAGGATT
ATGATCATATGGTGTTGGAGACTATTAGCTATTAGATTTTTCTCAAAATATGAGTTTTGTACAATGGAATGAAAAAGTGACTTCATATAC
GTAAGACTGGTGCCTAAGAATGACTTGAATGTTATCAGTACTACCACAGAACTATAATATACATTGCCTTTTCTCAGCAGCATTTGAATT
CAACTTATTTATTTCCTCTATTTGTAGCTTTTTGTTCTAGAGTCAGAACAGGTGGACTTTGCTTTGAATATTTTAGATGGGACAAAAATT
GTTCATCTTCGGAATTTATTTAAAGAAAGATCTACTGGTACAGGGTGTAAAAATACTTACAAGAATATGATTTTTTTGTTTGTTCATGCA
AACAGATCCTTTACCCAATATATATTCTGGAGGTAGGTATACTGGTGCAACCCATTTGCCAGCTGTGTGACTTTGAGTAAGTTCTTAATT
TCTCAGAGTTTCAGTGTTCTCATCTATAAAATTATGTCTCCAATTGTGAGTACCTAGCACTGTACCTGATACATAGTAAACTCACAATAA
ATATTTCTTAAACATAGATTTTGTTAGGAATACAGGCTTGATAACACAGTCCTTACCCTTACAATGTAATGTTAGAGATAAAGGAGCAAA
TAGAGTCACGCATCACTTAATGACAGAGATATGTTCTGAGAAATGCATCGTTAGGTGATATTGTCATTGTGTAAACATCATGTTGTTTAC
TTCCACAAACCTAGATGGTATAGCCTACCAGTACACCTAGGCTATATGGTATAGTCTGTTGCTCCTAGGCTACAAACCCGTATAGCATGT
TACTGTCCTGCATGCTATAGACAGTTGGAACATGTAGACTTGTATAAACACTGCACACTTAACCTATACTAAATTTATTGAAAATATTTT

>In-frame_ENST00000257075_ENST00000394367_TCGA-BR-8284-01A_PUM1_chr1_31532051_-_DDX52_chr17_35974398_length(amino acids)=135AA_start in transcript=94_stop in transcript=501
MSVACVLKRKAVLWQDSFSPHLKHHPQEPANPNMPVVLTSGTGSQAQPQPAANQALAAGTHSSPVPGSIGVAGRSQDDAMVDYFFQRQHG

--------------------------------------------------------------
>In-frame_ENST00000373747_ENST00000349699_TCGA-BR-8284-01A_PUM1_chr1_31532051_-_DDX52_chr17_35974398_length(transcript)=5036nt_BP=463nt
GTTGTCGGAGTGAAAGGTAAGGGGGAGCGAGAGCGCCAGAGAGAGAAGATCGGGGGGCTGAAATCCATCTTCATCCTACCGCTCCGCCCG
TGTTGGTGGAATGAGCGTTGCATGTGTCTTGAAGAGAAAAGCAGTGCTTTGGCAGGACTCTTTCAGCCCCCACCTGAAACATCACCCTCA
AGAACCAGCTAATCCCAACATGCCTGTTGTTTTGACATCTGGAACAGGGTCGCAAGCGCAGCCACAACCAGCTGCAAATCAGGCTCTTGC
AGCTGGGACTCACTCCAGCCCTGTCCCAGGATCTATAGGAGTTGCAGGCCGTTCCCAGGACGACGCTATGGTGGACTACTTCTTTCAGAG
GCAGCATGGTGAGCAGCTTGGGGGAGGAGGAAGTGGAGGAGGCGGCTATAATAATAGCAAACATCGATGGCCTACTGGGGATAACATTCA
TGCAGAACATCAGGAAAAAGGTCACTGGTCAGAACAGCAAGAAGAAAGTAGCTCTTGAAGACAAAAGTTAAAAACAGACTTTAAAAATAC
TGTCCCAGAAATGTAATTTTATGATCCCAGCATGAATGTTATTTTCATGGAATACTTGAAGTCTTACAGTCACCTGTACCAAACATTTGA
AATCAACTACAAGTACATGGGACTGGTGATAAATGATCCTAAACTATCAAGTCAGTTTCAATTTGTAGGTGCCTTTTTTTTTTCCTGTAG
AGATGAGGGTCTTGCCATGTTGTCCAGGCTGGTCTTGAACTCCTGACCTCACACAATCCTCCTGCCTTAGCCTCCTGAGTAACTGAGATT
ACAGGCACAAGCTGCTGCACCCAGCTCTGTAGGTGACTTTTAAATGATTATACAATGGAAATAACATTCATTGACATTTCTGTGGTTTGA
ATCCAGAGAGATACTTCTTATAGAAAAACAAATGTTTATGCTAAAAATAACACCAAAATGTGGTGAACTCTTAAGGACTTTTCCCTTCAA
GTGTGAAGGAAGGTGTGATGAATGCTGTGGAGAGGCATCTGGAACAGAAATTCAAAATAAAGCCTTGACATTAAATACCCCTTCCACTGC
TCACTTTGTGGATGGTAGCATGAGCTGTCTACCAAGAAGAAACCTGCTGCTCTCTTAATTTTAATATTTCCTAATTTGTTGATGGCCTTT
TGTGTTGTGAACCACAACAAAGAGAGGCCTCTTTTGTGGCTGGTTATTCCAGTTCCCTGGGATTTTAAATTCTTTGGTCTATTAAGTATC
CTTGTATTGGATACGTAATACCTTAGTGCTGTCATAATGTTGTACAAGATCATGATCAGCTTCTCCCTTTCTTCATTTTCTGTGATTTAA
CCATGTTCTTTCCTGTCTCTTTCCATTTAAGATATTTTATTTGAATACTGATAAACATTTTATCCTGATAAGGAAGAATGTTCTTGTTAC
TTGATATACCTCTGTCTTCATTCTCTTACAGCTTATCTTTCCTTAGGTTGATGGTGCCTCATTTAATAAGTAGATCTCTACTTAAGCTAA
AGGATTATGATCATATGGTGTTGGAGACTATTAGCTATTAGATTTTTCTCAAAATATGAGTTTTGTACAATGGAATGAAAAAGTGACTTC
ATATACGTAAGACTGGTGCCTAAGAATGACTTGAATGTTATCAGTACTACCACAGAACTATAATATACATTGCCTTTTCTCAGCAGCATT
TGAATTCAACTTATTTATTTCCTCTATTTGTAGCTTTTTGTTCTAGAGTCAGAACAGGTGGACTTTGCTTTGAATATTTTAGATGGGACA
AAAATTGTTCATCTTCGGAATTTATTTAAAGAAAGATCTACTGGTACAGGGTGTAAAAATACTTACAAGAATATGATTTTTTTGTTTGTT
CATGCAAACAGATCCTTTACCCAATATATATTCTGGAGGTAGGTATACTGGTGCAACCCATTTGCCAGCTGTGTGACTTTGAGTAAGTTC
TTAATTTCTCAGAGTTTCAGTGTTCTCATCTATAAAATTATGTCTCCAATTGTGAGTACCTAGCACTGTACCTGATACATAGTAAACTCA
CAATAAATATTTCTTAAACATAGATTTTGTTAGGAATACAGGCTTGATAACACAGTCCTTACCCTTACAATGTAATGTTAGAGATAAAGG
AGCAAATAGAGTCACGCATCACTTAATGACAGAGATATGTTCTGAGAAATGCATCGTTAGGTGATATTGTCATTGTGTAAACATCATGTT
GTTTACTTCCACAAACCTAGATGGTATAGCCTACCAGTACACCTAGGCTATATGGTATAGTCTGTTGCTCCTAGGCTACAAACCCGTATA
GCATGTTACTGTCCTGCATGCTATAGACAGTTGGAACATGTAGACTTGTATAAACACTGCACACTTAACCTATACTAAATTTATTGAAAA
TATTTTTCTTGGCCAGGCGTAGTGGCTCATGCCTGTAATCCTAACACTTCGGGAGGCCTAGGCGGGCAGGTTGTCTGAGCTCAGGAGTTT
AGAGACCAGCCCGGGCAACATGGCAAAACCCCATCTCTACTAAAAAAAATACCAAAAAAAGTTAGCCGGGTGTGGTGGCATAGTCCCGGC
TACTCAGGAGTCTGAGGCAGGAGAATAGCTTGAACCTGTGAGGCGGAGGTTGCAGTAAGCCGAGATCGCGCTACTGCACTCCAGCCTGGG
CGACAGGGCAAGACTGTCTTGAAAATAAACACACACACACACATTATATATATTATGTATTATATATATTAGATATATATTTTTTCTTCA
ATAAATTAACCTTAGCTTACTGTAACTTTTACTTTATGAGTTTTTTTGTTTTGTTTTGTTTTTTTTTTTTGAGAAACAGGGTCTGTTACC
TAATTTGGAGTTTAGTGGCACAATCATGGCCCACTGCAGCCTTGACCTCTTAGACTCAAGTGATCCTCAAACCTCAGCCTCCCGAGTAGC
TGGTACTATAGGTGCGGGCCACCAACACTCAGCTAATTTTTAAATTTTGTAGAGACGAGGTCTTGCTTGTTGTCCAGTTGGGTTTTTTAG
ACTTTTTTTTTTTTTTTTTTTTGGAGATGGAGTCTTGCTCTGTTGCCCAGGCTGGAGTGCAGTGGCATGATCTCGGCTCACTGCAACCTC
TGACTCCTGGGTTCAAGTGATTCTCCTGCCTCAGCCTCCCGAGTTGCTGGGACTACAGGCATGCGCCACCACACCCAGCTAATTTTTGTA
TTTTTAGTGACAGGGTTTCACCATGTTGGCCAGGCTGGTCTCAAACTCCTGACCTGGAGTGAGCCACTATATCTGGCCTTTATAAACTTT
TTTGATTCTTGTCATAACACTTAGCCTAAAATGCAAATGTACAGCTGTAGAAAAATACTTTATTTCTTTATATCCTTATTCTAGAAGCTT
TTTTTCTATTAATTTTTGTTTGTTTGTTTTGTTTTACTATTTACTTCTAAAACTTTTTTGTTAAAAACCATGGCACAAACACACACATTA
TGCTAGGCATACAAAAGGTCAGGATCATCAGTCTCACTGTCTTCCACCCCCACTTCTCATCCCACCATTGTATCTGCTGTCTGTGGCTGA
CCAAAACATCATCATGTAGCACATGACTAGTGTGGCAAGTGCTCTGTTAGATGTAAGGCCATGATGCTAAAGCATCACAAGAGGGCATCT
AACCCAGATTGGGGATGTCATGGAAGGCCGACATCCTGAGTTGAATCCTGCAAATGTAAAAACCAATAGGCAAAGAAGAGGAACAAAAAG
GATTCCAGGACAAACTGAGGTCACATCTATGATCCTTGACTTTATTGTGTCTGTTTAAAGTATCTACAGTAACCTGTATCAACTTAGTCA
GTGTATTAATACTAAATTTAGCTCCTTCAAAGCAGTTGGAACTATGTGCTACATAAATTTCAGCTTCACACAAGGAAGGGAAGGAGTGAA
ATTAGTGAACAGGCAGTTACAGCAAAAGAAAAAACATAAAAATTGAATAGCTGGCTCTGGTGAAATGAGCAAGGACTTTAGAGTCAAACT
GGCCTGGATTTGAATCCTGATCCTCATTGCTTGTAGCTGTATGATCTGGACAAATGACAGTAGCTGTTTCTAACCTTGATTTTCTCATCT
GTAAGATGCCAATTGTAACTCCTAAGGATACTGAGGATTTTTTAAAATGCGTGTACAGTTCCTGACCAGTGGTTTGTGCCTAATAACTTA
TTACAAATTATTACCCAGTAAAAACCTTGAGACAAGAGTGAAAACGTAAAGCTAATTAATCCATTACTTGTTAGCAAGCAAACTACGTGC
TTGAGAAAATTACTCAACTTTCATGTTTTACTTCCAGACAGTAGTTTGATTAAAAGAAAAAAAAAAATCACAGCCCAGGCATGTGGCTCA
CACCCTGGCACTTGGAGGCCGAGGTGGGAGCATAGCTTGAGCCTAGCAGTTTGAAACTAGCCTGGGCAACATAGATCCTATCTCTACAAA
AAAAAAAAAAAAAAAAAAAAAATTGGCCCGGTGTGGTGGTGCATGCCTGCGGTCCCAGCTACTCAGGAGGCTGAGGCGAGAGGATCGCTT
GAGCCAGGGAGGCTGAGGCTGCTGTGAGCCATGATCATGCCACTGCACTCCAGCCTGGACAACAGAGCAAGACCCTGTCTCAAAAAAAAA
AAAAAATCAGTAAAAAATATGCCGAAATAGCTTCAGAGTAATTATGAATTGCCTTCCAAAAACCACTGTTTTGCACTTGCCTGCAAGAAT
AGGAAAAACAAAAGGACTGTAAGGTGGCAGGATGGCAGAACCAAACAAGAGTAGTACACCTTGGAGTTTTTTCGAAATATGGGTTGGGTT
TTTGGGCTCTTGGTTGATTTAAAATAAAATTTAAATGCCTTCAGCGTCTGTGATAGGTGCCAACATGAAGCTACCGAGTTCCAGAGAAAG

>In-frame_ENST00000373747_ENST00000349699_TCGA-BR-8284-01A_PUM1_chr1_31532051_-_DDX52_chr17_35974398_length(amino acids)=135AA_start in transcript=100_stop in transcript=507
MSVACVLKRKAVLWQDSFSPHLKHHPQEPANPNMPVVLTSGTGSQAQPQPAANQALAAGTHSSPVPGSIGVAGRSQDDAMVDYFFQRQHG

--------------------------------------------------------------
>In-frame_ENST00000373747_ENST00000394367_TCGA-BR-8284-01A_PUM1_chr1_31532051_-_DDX52_chr17_35974398_length(transcript)=2449nt_BP=463nt
GTTGTCGGAGTGAAAGGTAAGGGGGAGCGAGAGCGCCAGAGAGAGAAGATCGGGGGGCTGAAATCCATCTTCATCCTACCGCTCCGCCCG
TGTTGGTGGAATGAGCGTTGCATGTGTCTTGAAGAGAAAAGCAGTGCTTTGGCAGGACTCTTTCAGCCCCCACCTGAAACATCACCCTCA
AGAACCAGCTAATCCCAACATGCCTGTTGTTTTGACATCTGGAACAGGGTCGCAAGCGCAGCCACAACCAGCTGCAAATCAGGCTCTTGC
AGCTGGGACTCACTCCAGCCCTGTCCCAGGATCTATAGGAGTTGCAGGCCGTTCCCAGGACGACGCTATGGTGGACTACTTCTTTCAGAG
GCAGCATGGTGAGCAGCTTGGGGGAGGAGGAAGTGGAGGAGGCGGCTATAATAATAGCAAACATCGATGGCCTACTGGGGATAACATTCA
TGCAGAACATCAGGAAAAAGGTCACTGGTCAGAACAGCAAGAAGAAAGTAGCTCTTGAAGACAAAAGTTAAAAACAGACTTTAAAAATAC
TGTCCCAGAAATGTAATTTTATGATCCCAGCATGAATGTTATTTTCATGGAATACTTGAAGTCTTACAGTCACCTGTACCAAACATTTGA
AATCAACTACAAGTACATGGGACTGGTGATAAATGATCCTAAACTATCAAGTCAGTTTCAATTTGTAGGTGCCTTTTTTTTTTCCTGTAG
AGATGAGGGTCTTGCCATGTTGTCCAGGCTGGTCTTGAACTCCTGACCTCACACAATCCTCCTGCCTTAGCCTCCTGAGTAACTGAGATT
ACAGGCACAAGCTGCTGCACCCAGCTCTGTAGGTGACTTTTAAATGATTATACAATGGAAATAACATTCATTGACATTTCTGTGGTTTGA
ATCCAGAGAGATACTTCTTATAGAAAAACAAATGTTTATGCTAAAAATAACACCAAAATGTGGTGAACTCTTAAGGACTTTTCCCTTCAA
GTGTGAAGGAAGGTGTGATGAATGCTGTGGAGAGGCATCTGGAACAGAAATTCAAAATAAAGCCTTGACATTAAATACCCCTTCCACTGC
TCACTTTGTGGATGGTAGCATGAGCTGTCTACCAAGAAGAAACCTGCTGCTCTCTTAATTTTAATATTTCCTAATTTGTTGATGGCCTTT
TGTGTTGTGAACCACAACAAAGAGAGGCCTCTTTTGTGGCTGGTTATTCCAGTTCCCTGGGATTTTAAATTCTTTGGTCTATTAAGTATC
CTTGTATTGGATACGTAATACCTTAGTGCTGTCATAATGTTGTACAAGATCATGATCAGCTTCTCCCTTTCTTCATTTTCTGTGATTTAA
CCATGTTCTTTCCTGTCTCTTTCCATTTAAGATATTTTATTTGAATACTGATAAACATTTTATCCTGATAAGGAAGAATGTTCTTGTTAC
TTGATATACCTCTGTCTTCATTCTCTTACAGCTTATCTTTCCTTAGGTTGATGGTGCCTCATTTAATAAGTAGATCTCTACTTAAGCTAA
AGGATTATGATCATATGGTGTTGGAGACTATTAGCTATTAGATTTTTCTCAAAATATGAGTTTTGTACAATGGAATGAAAAAGTGACTTC
ATATACGTAAGACTGGTGCCTAAGAATGACTTGAATGTTATCAGTACTACCACAGAACTATAATATACATTGCCTTTTCTCAGCAGCATT
TGAATTCAACTTATTTATTTCCTCTATTTGTAGCTTTTTGTTCTAGAGTCAGAACAGGTGGACTTTGCTTTGAATATTTTAGATGGGACA
AAAATTGTTCATCTTCGGAATTTATTTAAAGAAAGATCTACTGGTACAGGGTGTAAAAATACTTACAAGAATATGATTTTTTTGTTTGTT
CATGCAAACAGATCCTTTACCCAATATATATTCTGGAGGTAGGTATACTGGTGCAACCCATTTGCCAGCTGTGTGACTTTGAGTAAGTTC
TTAATTTCTCAGAGTTTCAGTGTTCTCATCTATAAAATTATGTCTCCAATTGTGAGTACCTAGCACTGTACCTGATACATAGTAAACTCA
CAATAAATATTTCTTAAACATAGATTTTGTTAGGAATACAGGCTTGATAACACAGTCCTTACCCTTACAATGTAATGTTAGAGATAAAGG
AGCAAATAGAGTCACGCATCACTTAATGACAGAGATATGTTCTGAGAAATGCATCGTTAGGTGATATTGTCATTGTGTAAACATCATGTT
GTTTACTTCCACAAACCTAGATGGTATAGCCTACCAGTACACCTAGGCTATATGGTATAGTCTGTTGCTCCTAGGCTACAAACCCGTATA
GCATGTTACTGTCCTGCATGCTATAGACAGTTGGAACATGTAGACTTGTATAAACACTGCACACTTAACCTATACTAAATTTATTGAAAA

>In-frame_ENST00000373747_ENST00000394367_TCGA-BR-8284-01A_PUM1_chr1_31532051_-_DDX52_chr17_35974398_length(amino acids)=135AA_start in transcript=100_stop in transcript=507
MSVACVLKRKAVLWQDSFSPHLKHHPQEPANPNMPVVLTSGTGSQAQPQPAANQALAAGTHSSPVPGSIGVAGRSQDDAMVDYFFQRQHG

--------------------------------------------------------------
>In-frame_ENST00000426105_ENST00000349699_TCGA-BR-8284-01A_PUM1_chr1_31532051_-_DDX52_chr17_35974398_length(transcript)=5027nt_BP=454nt
GTGAAAGGTAAGGGGGAGCGAGAGCGCCAGAGAGAGAAGATCGGGGGGCTGAAATCCATCTTCATCCTACCGCTCCGCCCGTGTTGGTGG
AATGAGCGTTGCATGTGTCTTGAAGAGAAAAGCAGTGCTTTGGCAGGACTCTTTCAGCCCCCACCTGAAACATCACCCTCAAGAACCAGC
TAATCCCAACATGCCTGTTGTTTTGACATCTGGAACAGGGTCGCAAGCGCAGCCACAACCAGCTGCAAATCAGGCTCTTGCAGCTGGGAC
TCACTCCAGCCCTGTCCCAGGATCTATAGGAGTTGCAGGCCGTTCCCAGGACGACGCTATGGTGGACTACTTCTTTCAGAGGCAGCATGG
TGAGCAGCTTGGGGGAGGAGGAAGTGGAGGAGGCGGCTATAATAATAGCAAACATCGATGGCCTACTGGGGATAACATTCATGCAGAACA
TCAGGAAAAAGGTCACTGGTCAGAACAGCAAGAAGAAAGTAGCTCTTGAAGACAAAAGTTAAAAACAGACTTTAAAAATACTGTCCCAGA
AATGTAATTTTATGATCCCAGCATGAATGTTATTTTCATGGAATACTTGAAGTCTTACAGTCACCTGTACCAAACATTTGAAATCAACTA
CAAGTACATGGGACTGGTGATAAATGATCCTAAACTATCAAGTCAGTTTCAATTTGTAGGTGCCTTTTTTTTTTCCTGTAGAGATGAGGG
TCTTGCCATGTTGTCCAGGCTGGTCTTGAACTCCTGACCTCACACAATCCTCCTGCCTTAGCCTCCTGAGTAACTGAGATTACAGGCACA
AGCTGCTGCACCCAGCTCTGTAGGTGACTTTTAAATGATTATACAATGGAAATAACATTCATTGACATTTCTGTGGTTTGAATCCAGAGA
GATACTTCTTATAGAAAAACAAATGTTTATGCTAAAAATAACACCAAAATGTGGTGAACTCTTAAGGACTTTTCCCTTCAAGTGTGAAGG
AAGGTGTGATGAATGCTGTGGAGAGGCATCTGGAACAGAAATTCAAAATAAAGCCTTGACATTAAATACCCCTTCCACTGCTCACTTTGT
GGATGGTAGCATGAGCTGTCTACCAAGAAGAAACCTGCTGCTCTCTTAATTTTAATATTTCCTAATTTGTTGATGGCCTTTTGTGTTGTG
AACCACAACAAAGAGAGGCCTCTTTTGTGGCTGGTTATTCCAGTTCCCTGGGATTTTAAATTCTTTGGTCTATTAAGTATCCTTGTATTG
GATACGTAATACCTTAGTGCTGTCATAATGTTGTACAAGATCATGATCAGCTTCTCCCTTTCTTCATTTTCTGTGATTTAACCATGTTCT
TTCCTGTCTCTTTCCATTTAAGATATTTTATTTGAATACTGATAAACATTTTATCCTGATAAGGAAGAATGTTCTTGTTACTTGATATAC
CTCTGTCTTCATTCTCTTACAGCTTATCTTTCCTTAGGTTGATGGTGCCTCATTTAATAAGTAGATCTCTACTTAAGCTAAAGGATTATG
ATCATATGGTGTTGGAGACTATTAGCTATTAGATTTTTCTCAAAATATGAGTTTTGTACAATGGAATGAAAAAGTGACTTCATATACGTA
AGACTGGTGCCTAAGAATGACTTGAATGTTATCAGTACTACCACAGAACTATAATATACATTGCCTTTTCTCAGCAGCATTTGAATTCAA
CTTATTTATTTCCTCTATTTGTAGCTTTTTGTTCTAGAGTCAGAACAGGTGGACTTTGCTTTGAATATTTTAGATGGGACAAAAATTGTT
CATCTTCGGAATTTATTTAAAGAAAGATCTACTGGTACAGGGTGTAAAAATACTTACAAGAATATGATTTTTTTGTTTGTTCATGCAAAC
AGATCCTTTACCCAATATATATTCTGGAGGTAGGTATACTGGTGCAACCCATTTGCCAGCTGTGTGACTTTGAGTAAGTTCTTAATTTCT
CAGAGTTTCAGTGTTCTCATCTATAAAATTATGTCTCCAATTGTGAGTACCTAGCACTGTACCTGATACATAGTAAACTCACAATAAATA
TTTCTTAAACATAGATTTTGTTAGGAATACAGGCTTGATAACACAGTCCTTACCCTTACAATGTAATGTTAGAGATAAAGGAGCAAATAG
AGTCACGCATCACTTAATGACAGAGATATGTTCTGAGAAATGCATCGTTAGGTGATATTGTCATTGTGTAAACATCATGTTGTTTACTTC
CACAAACCTAGATGGTATAGCCTACCAGTACACCTAGGCTATATGGTATAGTCTGTTGCTCCTAGGCTACAAACCCGTATAGCATGTTAC
TGTCCTGCATGCTATAGACAGTTGGAACATGTAGACTTGTATAAACACTGCACACTTAACCTATACTAAATTTATTGAAAATATTTTTCT
TGGCCAGGCGTAGTGGCTCATGCCTGTAATCCTAACACTTCGGGAGGCCTAGGCGGGCAGGTTGTCTGAGCTCAGGAGTTTAGAGACCAG
CCCGGGCAACATGGCAAAACCCCATCTCTACTAAAAAAAATACCAAAAAAAGTTAGCCGGGTGTGGTGGCATAGTCCCGGCTACTCAGGA
GTCTGAGGCAGGAGAATAGCTTGAACCTGTGAGGCGGAGGTTGCAGTAAGCCGAGATCGCGCTACTGCACTCCAGCCTGGGCGACAGGGC
AAGACTGTCTTGAAAATAAACACACACACACACATTATATATATTATGTATTATATATATTAGATATATATTTTTTCTTCAATAAATTAA
CCTTAGCTTACTGTAACTTTTACTTTATGAGTTTTTTTGTTTTGTTTTGTTTTTTTTTTTTGAGAAACAGGGTCTGTTACCTAATTTGGA
GTTTAGTGGCACAATCATGGCCCACTGCAGCCTTGACCTCTTAGACTCAAGTGATCCTCAAACCTCAGCCTCCCGAGTAGCTGGTACTAT
AGGTGCGGGCCACCAACACTCAGCTAATTTTTAAATTTTGTAGAGACGAGGTCTTGCTTGTTGTCCAGTTGGGTTTTTTAGACTTTTTTT
TTTTTTTTTTTTTGGAGATGGAGTCTTGCTCTGTTGCCCAGGCTGGAGTGCAGTGGCATGATCTCGGCTCACTGCAACCTCTGACTCCTG
GGTTCAAGTGATTCTCCTGCCTCAGCCTCCCGAGTTGCTGGGACTACAGGCATGCGCCACCACACCCAGCTAATTTTTGTATTTTTAGTG
ACAGGGTTTCACCATGTTGGCCAGGCTGGTCTCAAACTCCTGACCTGGAGTGAGCCACTATATCTGGCCTTTATAAACTTTTTTGATTCT
TGTCATAACACTTAGCCTAAAATGCAAATGTACAGCTGTAGAAAAATACTTTATTTCTTTATATCCTTATTCTAGAAGCTTTTTTTCTAT
TAATTTTTGTTTGTTTGTTTTGTTTTACTATTTACTTCTAAAACTTTTTTGTTAAAAACCATGGCACAAACACACACATTATGCTAGGCA
TACAAAAGGTCAGGATCATCAGTCTCACTGTCTTCCACCCCCACTTCTCATCCCACCATTGTATCTGCTGTCTGTGGCTGACCAAAACAT
CATCATGTAGCACATGACTAGTGTGGCAAGTGCTCTGTTAGATGTAAGGCCATGATGCTAAAGCATCACAAGAGGGCATCTAACCCAGAT
TGGGGATGTCATGGAAGGCCGACATCCTGAGTTGAATCCTGCAAATGTAAAAACCAATAGGCAAAGAAGAGGAACAAAAAGGATTCCAGG
ACAAACTGAGGTCACATCTATGATCCTTGACTTTATTGTGTCTGTTTAAAGTATCTACAGTAACCTGTATCAACTTAGTCAGTGTATTAA
TACTAAATTTAGCTCCTTCAAAGCAGTTGGAACTATGTGCTACATAAATTTCAGCTTCACACAAGGAAGGGAAGGAGTGAAATTAGTGAA
CAGGCAGTTACAGCAAAAGAAAAAACATAAAAATTGAATAGCTGGCTCTGGTGAAATGAGCAAGGACTTTAGAGTCAAACTGGCCTGGAT
TTGAATCCTGATCCTCATTGCTTGTAGCTGTATGATCTGGACAAATGACAGTAGCTGTTTCTAACCTTGATTTTCTCATCTGTAAGATGC
CAATTGTAACTCCTAAGGATACTGAGGATTTTTTAAAATGCGTGTACAGTTCCTGACCAGTGGTTTGTGCCTAATAACTTATTACAAATT
ATTACCCAGTAAAAACCTTGAGACAAGAGTGAAAACGTAAAGCTAATTAATCCATTACTTGTTAGCAAGCAAACTACGTGCTTGAGAAAA
TTACTCAACTTTCATGTTTTACTTCCAGACAGTAGTTTGATTAAAAGAAAAAAAAAAATCACAGCCCAGGCATGTGGCTCACACCCTGGC
ACTTGGAGGCCGAGGTGGGAGCATAGCTTGAGCCTAGCAGTTTGAAACTAGCCTGGGCAACATAGATCCTATCTCTACAAAAAAAAAAAA
AAAAAAAAAAAAATTGGCCCGGTGTGGTGGTGCATGCCTGCGGTCCCAGCTACTCAGGAGGCTGAGGCGAGAGGATCGCTTGAGCCAGGG
AGGCTGAGGCTGCTGTGAGCCATGATCATGCCACTGCACTCCAGCCTGGACAACAGAGCAAGACCCTGTCTCAAAAAAAAAAAAAAATCA
GTAAAAAATATGCCGAAATAGCTTCAGAGTAATTATGAATTGCCTTCCAAAAACCACTGTTTTGCACTTGCCTGCAAGAATAGGAAAAAC
AAAAGGACTGTAAGGTGGCAGGATGGCAGAACCAAACAAGAGTAGTACACCTTGGAGTTTTTTCGAAATATGGGTTGGGTTTTTGGGCTC
TTGGTTGATTTAAAATAAAATTTAAATGCCTTCAGCGTCTGTGATAGGTGCCAACATGAAGCTACCGAGTTCCAGAGAAAGAGGGGAGAG

>In-frame_ENST00000426105_ENST00000349699_TCGA-BR-8284-01A_PUM1_chr1_31532051_-_DDX52_chr17_35974398_length(amino acids)=135AA_start in transcript=91_stop in transcript=498
MSVACVLKRKAVLWQDSFSPHLKHHPQEPANPNMPVVLTSGTGSQAQPQPAANQALAAGTHSSPVPGSIGVAGRSQDDAMVDYFFQRQHG

--------------------------------------------------------------
>In-frame_ENST00000426105_ENST00000394367_TCGA-BR-8284-01A_PUM1_chr1_31532051_-_DDX52_chr17_35974398_length(transcript)=2440nt_BP=454nt
GTGAAAGGTAAGGGGGAGCGAGAGCGCCAGAGAGAGAAGATCGGGGGGCTGAAATCCATCTTCATCCTACCGCTCCGCCCGTGTTGGTGG
AATGAGCGTTGCATGTGTCTTGAAGAGAAAAGCAGTGCTTTGGCAGGACTCTTTCAGCCCCCACCTGAAACATCACCCTCAAGAACCAGC
TAATCCCAACATGCCTGTTGTTTTGACATCTGGAACAGGGTCGCAAGCGCAGCCACAACCAGCTGCAAATCAGGCTCTTGCAGCTGGGAC
TCACTCCAGCCCTGTCCCAGGATCTATAGGAGTTGCAGGCCGTTCCCAGGACGACGCTATGGTGGACTACTTCTTTCAGAGGCAGCATGG
TGAGCAGCTTGGGGGAGGAGGAAGTGGAGGAGGCGGCTATAATAATAGCAAACATCGATGGCCTACTGGGGATAACATTCATGCAGAACA
TCAGGAAAAAGGTCACTGGTCAGAACAGCAAGAAGAAAGTAGCTCTTGAAGACAAAAGTTAAAAACAGACTTTAAAAATACTGTCCCAGA
AATGTAATTTTATGATCCCAGCATGAATGTTATTTTCATGGAATACTTGAAGTCTTACAGTCACCTGTACCAAACATTTGAAATCAACTA
CAAGTACATGGGACTGGTGATAAATGATCCTAAACTATCAAGTCAGTTTCAATTTGTAGGTGCCTTTTTTTTTTCCTGTAGAGATGAGGG
TCTTGCCATGTTGTCCAGGCTGGTCTTGAACTCCTGACCTCACACAATCCTCCTGCCTTAGCCTCCTGAGTAACTGAGATTACAGGCACA
AGCTGCTGCACCCAGCTCTGTAGGTGACTTTTAAATGATTATACAATGGAAATAACATTCATTGACATTTCTGTGGTTTGAATCCAGAGA
GATACTTCTTATAGAAAAACAAATGTTTATGCTAAAAATAACACCAAAATGTGGTGAACTCTTAAGGACTTTTCCCTTCAAGTGTGAAGG
AAGGTGTGATGAATGCTGTGGAGAGGCATCTGGAACAGAAATTCAAAATAAAGCCTTGACATTAAATACCCCTTCCACTGCTCACTTTGT
GGATGGTAGCATGAGCTGTCTACCAAGAAGAAACCTGCTGCTCTCTTAATTTTAATATTTCCTAATTTGTTGATGGCCTTTTGTGTTGTG
AACCACAACAAAGAGAGGCCTCTTTTGTGGCTGGTTATTCCAGTTCCCTGGGATTTTAAATTCTTTGGTCTATTAAGTATCCTTGTATTG
GATACGTAATACCTTAGTGCTGTCATAATGTTGTACAAGATCATGATCAGCTTCTCCCTTTCTTCATTTTCTGTGATTTAACCATGTTCT
TTCCTGTCTCTTTCCATTTAAGATATTTTATTTGAATACTGATAAACATTTTATCCTGATAAGGAAGAATGTTCTTGTTACTTGATATAC
CTCTGTCTTCATTCTCTTACAGCTTATCTTTCCTTAGGTTGATGGTGCCTCATTTAATAAGTAGATCTCTACTTAAGCTAAAGGATTATG
ATCATATGGTGTTGGAGACTATTAGCTATTAGATTTTTCTCAAAATATGAGTTTTGTACAATGGAATGAAAAAGTGACTTCATATACGTA
AGACTGGTGCCTAAGAATGACTTGAATGTTATCAGTACTACCACAGAACTATAATATACATTGCCTTTTCTCAGCAGCATTTGAATTCAA
CTTATTTATTTCCTCTATTTGTAGCTTTTTGTTCTAGAGTCAGAACAGGTGGACTTTGCTTTGAATATTTTAGATGGGACAAAAATTGTT
CATCTTCGGAATTTATTTAAAGAAAGATCTACTGGTACAGGGTGTAAAAATACTTACAAGAATATGATTTTTTTGTTTGTTCATGCAAAC
AGATCCTTTACCCAATATATATTCTGGAGGTAGGTATACTGGTGCAACCCATTTGCCAGCTGTGTGACTTTGAGTAAGTTCTTAATTTCT
CAGAGTTTCAGTGTTCTCATCTATAAAATTATGTCTCCAATTGTGAGTACCTAGCACTGTACCTGATACATAGTAAACTCACAATAAATA
TTTCTTAAACATAGATTTTGTTAGGAATACAGGCTTGATAACACAGTCCTTACCCTTACAATGTAATGTTAGAGATAAAGGAGCAAATAG
AGTCACGCATCACTTAATGACAGAGATATGTTCTGAGAAATGCATCGTTAGGTGATATTGTCATTGTGTAAACATCATGTTGTTTACTTC
CACAAACCTAGATGGTATAGCCTACCAGTACACCTAGGCTATATGGTATAGTCTGTTGCTCCTAGGCTACAAACCCGTATAGCATGTTAC
TGTCCTGCATGCTATAGACAGTTGGAACATGTAGACTTGTATAAACACTGCACACTTAACCTATACTAAATTTATTGAAAATATTTTTCT

>In-frame_ENST00000426105_ENST00000394367_TCGA-BR-8284-01A_PUM1_chr1_31532051_-_DDX52_chr17_35974398_length(amino acids)=135AA_start in transcript=91_stop in transcript=498
MSVACVLKRKAVLWQDSFSPHLKHHPQEPANPNMPVVLTSGTGSQAQPQPAANQALAAGTHSSPVPGSIGVAGRSQDDAMVDYFFQRQHG

--------------------------------------------------------------
>In-frame_ENST00000440538_ENST00000349699_TCGA-BR-8284-01A_PUM1_chr1_31532051_-_DDX52_chr17_35974398_length(transcript)=5016nt_BP=443nt
GGGGGAGCGAGAGCGCCAGAGAGAGAAGATCGGGGGGCTGAAATCCATCTTCATCCTACCGCTCCGCCCGTGTTGGTGGAATGAGCGTTG
CATGTGTCTTGAAGAGAAAAGCAGTGCTTTGGCAGGACTCTTTCAGCCCCCACCTGAAACATCACCCTCAAGAACCAGCTAATCCCAACA
TGCCTGTTGTTTTGACATCTGGAACAGGGTCGCAAGCGCAGCCACAACCAGCTGCAAATCAGGCTCTTGCAGCTGGGACTCACTCCAGCC
CTGTCCCAGGATCTATAGGAGTTGCAGGCCGTTCCCAGGACGACGCTATGGTGGACTACTTCTTTCAGAGGCAGCATGGTGAGCAGCTTG
GGGGAGGAGGAAGTGGAGGAGGCGGCTATAATAATAGCAAACATCGATGGCCTACTGGGGATAACATTCATGCAGAACATCAGGAAAAAG
GTCACTGGTCAGAACAGCAAGAAGAAAGTAGCTCTTGAAGACAAAAGTTAAAAACAGACTTTAAAAATACTGTCCCAGAAATGTAATTTT
ATGATCCCAGCATGAATGTTATTTTCATGGAATACTTGAAGTCTTACAGTCACCTGTACCAAACATTTGAAATCAACTACAAGTACATGG
GACTGGTGATAAATGATCCTAAACTATCAAGTCAGTTTCAATTTGTAGGTGCCTTTTTTTTTTCCTGTAGAGATGAGGGTCTTGCCATGT
TGTCCAGGCTGGTCTTGAACTCCTGACCTCACACAATCCTCCTGCCTTAGCCTCCTGAGTAACTGAGATTACAGGCACAAGCTGCTGCAC
CCAGCTCTGTAGGTGACTTTTAAATGATTATACAATGGAAATAACATTCATTGACATTTCTGTGGTTTGAATCCAGAGAGATACTTCTTA
TAGAAAAACAAATGTTTATGCTAAAAATAACACCAAAATGTGGTGAACTCTTAAGGACTTTTCCCTTCAAGTGTGAAGGAAGGTGTGATG
AATGCTGTGGAGAGGCATCTGGAACAGAAATTCAAAATAAAGCCTTGACATTAAATACCCCTTCCACTGCTCACTTTGTGGATGGTAGCA
TGAGCTGTCTACCAAGAAGAAACCTGCTGCTCTCTTAATTTTAATATTTCCTAATTTGTTGATGGCCTTTTGTGTTGTGAACCACAACAA
AGAGAGGCCTCTTTTGTGGCTGGTTATTCCAGTTCCCTGGGATTTTAAATTCTTTGGTCTATTAAGTATCCTTGTATTGGATACGTAATA
CCTTAGTGCTGTCATAATGTTGTACAAGATCATGATCAGCTTCTCCCTTTCTTCATTTTCTGTGATTTAACCATGTTCTTTCCTGTCTCT
TTCCATTTAAGATATTTTATTTGAATACTGATAAACATTTTATCCTGATAAGGAAGAATGTTCTTGTTACTTGATATACCTCTGTCTTCA
TTCTCTTACAGCTTATCTTTCCTTAGGTTGATGGTGCCTCATTTAATAAGTAGATCTCTACTTAAGCTAAAGGATTATGATCATATGGTG
TTGGAGACTATTAGCTATTAGATTTTTCTCAAAATATGAGTTTTGTACAATGGAATGAAAAAGTGACTTCATATACGTAAGACTGGTGCC
TAAGAATGACTTGAATGTTATCAGTACTACCACAGAACTATAATATACATTGCCTTTTCTCAGCAGCATTTGAATTCAACTTATTTATTT
CCTCTATTTGTAGCTTTTTGTTCTAGAGTCAGAACAGGTGGACTTTGCTTTGAATATTTTAGATGGGACAAAAATTGTTCATCTTCGGAA
TTTATTTAAAGAAAGATCTACTGGTACAGGGTGTAAAAATACTTACAAGAATATGATTTTTTTGTTTGTTCATGCAAACAGATCCTTTAC
CCAATATATATTCTGGAGGTAGGTATACTGGTGCAACCCATTTGCCAGCTGTGTGACTTTGAGTAAGTTCTTAATTTCTCAGAGTTTCAG
TGTTCTCATCTATAAAATTATGTCTCCAATTGTGAGTACCTAGCACTGTACCTGATACATAGTAAACTCACAATAAATATTTCTTAAACA
TAGATTTTGTTAGGAATACAGGCTTGATAACACAGTCCTTACCCTTACAATGTAATGTTAGAGATAAAGGAGCAAATAGAGTCACGCATC
ACTTAATGACAGAGATATGTTCTGAGAAATGCATCGTTAGGTGATATTGTCATTGTGTAAACATCATGTTGTTTACTTCCACAAACCTAG
ATGGTATAGCCTACCAGTACACCTAGGCTATATGGTATAGTCTGTTGCTCCTAGGCTACAAACCCGTATAGCATGTTACTGTCCTGCATG
CTATAGACAGTTGGAACATGTAGACTTGTATAAACACTGCACACTTAACCTATACTAAATTTATTGAAAATATTTTTCTTGGCCAGGCGT
AGTGGCTCATGCCTGTAATCCTAACACTTCGGGAGGCCTAGGCGGGCAGGTTGTCTGAGCTCAGGAGTTTAGAGACCAGCCCGGGCAACA
TGGCAAAACCCCATCTCTACTAAAAAAAATACCAAAAAAAGTTAGCCGGGTGTGGTGGCATAGTCCCGGCTACTCAGGAGTCTGAGGCAG
GAGAATAGCTTGAACCTGTGAGGCGGAGGTTGCAGTAAGCCGAGATCGCGCTACTGCACTCCAGCCTGGGCGACAGGGCAAGACTGTCTT
GAAAATAAACACACACACACACATTATATATATTATGTATTATATATATTAGATATATATTTTTTCTTCAATAAATTAACCTTAGCTTAC
TGTAACTTTTACTTTATGAGTTTTTTTGTTTTGTTTTGTTTTTTTTTTTTGAGAAACAGGGTCTGTTACCTAATTTGGAGTTTAGTGGCA
CAATCATGGCCCACTGCAGCCTTGACCTCTTAGACTCAAGTGATCCTCAAACCTCAGCCTCCCGAGTAGCTGGTACTATAGGTGCGGGCC
ACCAACACTCAGCTAATTTTTAAATTTTGTAGAGACGAGGTCTTGCTTGTTGTCCAGTTGGGTTTTTTAGACTTTTTTTTTTTTTTTTTT
TTGGAGATGGAGTCTTGCTCTGTTGCCCAGGCTGGAGTGCAGTGGCATGATCTCGGCTCACTGCAACCTCTGACTCCTGGGTTCAAGTGA
TTCTCCTGCCTCAGCCTCCCGAGTTGCTGGGACTACAGGCATGCGCCACCACACCCAGCTAATTTTTGTATTTTTAGTGACAGGGTTTCA
CCATGTTGGCCAGGCTGGTCTCAAACTCCTGACCTGGAGTGAGCCACTATATCTGGCCTTTATAAACTTTTTTGATTCTTGTCATAACAC
TTAGCCTAAAATGCAAATGTACAGCTGTAGAAAAATACTTTATTTCTTTATATCCTTATTCTAGAAGCTTTTTTTCTATTAATTTTTGTT
TGTTTGTTTTGTTTTACTATTTACTTCTAAAACTTTTTTGTTAAAAACCATGGCACAAACACACACATTATGCTAGGCATACAAAAGGTC
AGGATCATCAGTCTCACTGTCTTCCACCCCCACTTCTCATCCCACCATTGTATCTGCTGTCTGTGGCTGACCAAAACATCATCATGTAGC
ACATGACTAGTGTGGCAAGTGCTCTGTTAGATGTAAGGCCATGATGCTAAAGCATCACAAGAGGGCATCTAACCCAGATTGGGGATGTCA
TGGAAGGCCGACATCCTGAGTTGAATCCTGCAAATGTAAAAACCAATAGGCAAAGAAGAGGAACAAAAAGGATTCCAGGACAAACTGAGG
TCACATCTATGATCCTTGACTTTATTGTGTCTGTTTAAAGTATCTACAGTAACCTGTATCAACTTAGTCAGTGTATTAATACTAAATTTA
GCTCCTTCAAAGCAGTTGGAACTATGTGCTACATAAATTTCAGCTTCACACAAGGAAGGGAAGGAGTGAAATTAGTGAACAGGCAGTTAC
AGCAAAAGAAAAAACATAAAAATTGAATAGCTGGCTCTGGTGAAATGAGCAAGGACTTTAGAGTCAAACTGGCCTGGATTTGAATCCTGA
TCCTCATTGCTTGTAGCTGTATGATCTGGACAAATGACAGTAGCTGTTTCTAACCTTGATTTTCTCATCTGTAAGATGCCAATTGTAACT
CCTAAGGATACTGAGGATTTTTTAAAATGCGTGTACAGTTCCTGACCAGTGGTTTGTGCCTAATAACTTATTACAAATTATTACCCAGTA
AAAACCTTGAGACAAGAGTGAAAACGTAAAGCTAATTAATCCATTACTTGTTAGCAAGCAAACTACGTGCTTGAGAAAATTACTCAACTT
TCATGTTTTACTTCCAGACAGTAGTTTGATTAAAAGAAAAAAAAAAATCACAGCCCAGGCATGTGGCTCACACCCTGGCACTTGGAGGCC
GAGGTGGGAGCATAGCTTGAGCCTAGCAGTTTGAAACTAGCCTGGGCAACATAGATCCTATCTCTACAAAAAAAAAAAAAAAAAAAAAAA
AATTGGCCCGGTGTGGTGGTGCATGCCTGCGGTCCCAGCTACTCAGGAGGCTGAGGCGAGAGGATCGCTTGAGCCAGGGAGGCTGAGGCT
GCTGTGAGCCATGATCATGCCACTGCACTCCAGCCTGGACAACAGAGCAAGACCCTGTCTCAAAAAAAAAAAAAAATCAGTAAAAAATAT
GCCGAAATAGCTTCAGAGTAATTATGAATTGCCTTCCAAAAACCACTGTTTTGCACTTGCCTGCAAGAATAGGAAAAACAAAAGGACTGT
AAGGTGGCAGGATGGCAGAACCAAACAAGAGTAGTACACCTTGGAGTTTTTTCGAAATATGGGTTGGGTTTTTGGGCTCTTGGTTGATTT
AAAATAAAATTTAAATGCCTTCAGCGTCTGTGATAGGTGCCAACATGAAGCTACCGAGTTCCAGAGAAAGAGGGGAGAGCTGATCACTCA

>In-frame_ENST00000440538_ENST00000349699_TCGA-BR-8284-01A_PUM1_chr1_31532051_-_DDX52_chr17_35974398_length(amino acids)=135AA_start in transcript=80_stop in transcript=487
MSVACVLKRKAVLWQDSFSPHLKHHPQEPANPNMPVVLTSGTGSQAQPQPAANQALAAGTHSSPVPGSIGVAGRSQDDAMVDYFFQRQHG

--------------------------------------------------------------
>In-frame_ENST00000440538_ENST00000394367_TCGA-BR-8284-01A_PUM1_chr1_31532051_-_DDX52_chr17_35974398_length(transcript)=2429nt_BP=443nt
GGGGGAGCGAGAGCGCCAGAGAGAGAAGATCGGGGGGCTGAAATCCATCTTCATCCTACCGCTCCGCCCGTGTTGGTGGAATGAGCGTTG
CATGTGTCTTGAAGAGAAAAGCAGTGCTTTGGCAGGACTCTTTCAGCCCCCACCTGAAACATCACCCTCAAGAACCAGCTAATCCCAACA
TGCCTGTTGTTTTGACATCTGGAACAGGGTCGCAAGCGCAGCCACAACCAGCTGCAAATCAGGCTCTTGCAGCTGGGACTCACTCCAGCC
CTGTCCCAGGATCTATAGGAGTTGCAGGCCGTTCCCAGGACGACGCTATGGTGGACTACTTCTTTCAGAGGCAGCATGGTGAGCAGCTTG
GGGGAGGAGGAAGTGGAGGAGGCGGCTATAATAATAGCAAACATCGATGGCCTACTGGGGATAACATTCATGCAGAACATCAGGAAAAAG
GTCACTGGTCAGAACAGCAAGAAGAAAGTAGCTCTTGAAGACAAAAGTTAAAAACAGACTTTAAAAATACTGTCCCAGAAATGTAATTTT
ATGATCCCAGCATGAATGTTATTTTCATGGAATACTTGAAGTCTTACAGTCACCTGTACCAAACATTTGAAATCAACTACAAGTACATGG
GACTGGTGATAAATGATCCTAAACTATCAAGTCAGTTTCAATTTGTAGGTGCCTTTTTTTTTTCCTGTAGAGATGAGGGTCTTGCCATGT
TGTCCAGGCTGGTCTTGAACTCCTGACCTCACACAATCCTCCTGCCTTAGCCTCCTGAGTAACTGAGATTACAGGCACAAGCTGCTGCAC
CCAGCTCTGTAGGTGACTTTTAAATGATTATACAATGGAAATAACATTCATTGACATTTCTGTGGTTTGAATCCAGAGAGATACTTCTTA
TAGAAAAACAAATGTTTATGCTAAAAATAACACCAAAATGTGGTGAACTCTTAAGGACTTTTCCCTTCAAGTGTGAAGGAAGGTGTGATG
AATGCTGTGGAGAGGCATCTGGAACAGAAATTCAAAATAAAGCCTTGACATTAAATACCCCTTCCACTGCTCACTTTGTGGATGGTAGCA
TGAGCTGTCTACCAAGAAGAAACCTGCTGCTCTCTTAATTTTAATATTTCCTAATTTGTTGATGGCCTTTTGTGTTGTGAACCACAACAA
AGAGAGGCCTCTTTTGTGGCTGGTTATTCCAGTTCCCTGGGATTTTAAATTCTTTGGTCTATTAAGTATCCTTGTATTGGATACGTAATA
CCTTAGTGCTGTCATAATGTTGTACAAGATCATGATCAGCTTCTCCCTTTCTTCATTTTCTGTGATTTAACCATGTTCTTTCCTGTCTCT
TTCCATTTAAGATATTTTATTTGAATACTGATAAACATTTTATCCTGATAAGGAAGAATGTTCTTGTTACTTGATATACCTCTGTCTTCA
TTCTCTTACAGCTTATCTTTCCTTAGGTTGATGGTGCCTCATTTAATAAGTAGATCTCTACTTAAGCTAAAGGATTATGATCATATGGTG
TTGGAGACTATTAGCTATTAGATTTTTCTCAAAATATGAGTTTTGTACAATGGAATGAAAAAGTGACTTCATATACGTAAGACTGGTGCC
TAAGAATGACTTGAATGTTATCAGTACTACCACAGAACTATAATATACATTGCCTTTTCTCAGCAGCATTTGAATTCAACTTATTTATTT
CCTCTATTTGTAGCTTTTTGTTCTAGAGTCAGAACAGGTGGACTTTGCTTTGAATATTTTAGATGGGACAAAAATTGTTCATCTTCGGAA
TTTATTTAAAGAAAGATCTACTGGTACAGGGTGTAAAAATACTTACAAGAATATGATTTTTTTGTTTGTTCATGCAAACAGATCCTTTAC
CCAATATATATTCTGGAGGTAGGTATACTGGTGCAACCCATTTGCCAGCTGTGTGACTTTGAGTAAGTTCTTAATTTCTCAGAGTTTCAG
TGTTCTCATCTATAAAATTATGTCTCCAATTGTGAGTACCTAGCACTGTACCTGATACATAGTAAACTCACAATAAATATTTCTTAAACA
TAGATTTTGTTAGGAATACAGGCTTGATAACACAGTCCTTACCCTTACAATGTAATGTTAGAGATAAAGGAGCAAATAGAGTCACGCATC
ACTTAATGACAGAGATATGTTCTGAGAAATGCATCGTTAGGTGATATTGTCATTGTGTAAACATCATGTTGTTTACTTCCACAAACCTAG
ATGGTATAGCCTACCAGTACACCTAGGCTATATGGTATAGTCTGTTGCTCCTAGGCTACAAACCCGTATAGCATGTTACTGTCCTGCATG

>In-frame_ENST00000440538_ENST00000394367_TCGA-BR-8284-01A_PUM1_chr1_31532051_-_DDX52_chr17_35974398_length(amino acids)=135AA_start in transcript=80_stop in transcript=487
MSVACVLKRKAVLWQDSFSPHLKHHPQEPANPNMPVVLTSGTGSQAQPQPAANQALAAGTHSSPVPGSIGVAGRSQDDAMVDYFFQRQHG

--------------------------------------------------------------
>In-frame_ENST00000423018_ENST00000349699_TCGA-BR-8284-01A_PUM1_chr1_31532051_-_DDX52_chr17_35974398_length(transcript)=5025nt_BP=452nt
GAAAGGTAAGGGGGAGCGAGAGCGCCAGAGAGAGAAGATCGGGGGGCTGAAATCCATCTTCATCCTACCGCTCCGCCCGTGTTGGTGGAA
TGAGCGTTGCATGTGTCTTGAAGAGAAAAGCAGTGCTTTGGCAGGACTCTTTCAGCCCCCACCTGAAACATCACCCTCAAGAACCAGCTA
ATCCCAACATGCCTGTTGTTTTGACATCTGGAACAGGGTCGCAAGCGCAGCCACAACCAGCTGCAAATCAGGCTCTTGCAGCTGGGACTC
ACTCCAGCCCTGTCCCAGGATCTATAGGAGTTGCAGGCCGTTCCCAGGACGACGCTATGGTGGACTACTTCTTTCAGAGGCAGCATGGTG
AGCAGCTTGGGGGAGGAGGAAGTGGAGGAGGCGGCTATAATAATAGCAAACATCGATGGCCTACTGGGGATAACATTCATGCAGAACATC
AGGAAAAAGGTCACTGGTCAGAACAGCAAGAAGAAAGTAGCTCTTGAAGACAAAAGTTAAAAACAGACTTTAAAAATACTGTCCCAGAAA
TGTAATTTTATGATCCCAGCATGAATGTTATTTTCATGGAATACTTGAAGTCTTACAGTCACCTGTACCAAACATTTGAAATCAACTACA
AGTACATGGGACTGGTGATAAATGATCCTAAACTATCAAGTCAGTTTCAATTTGTAGGTGCCTTTTTTTTTTCCTGTAGAGATGAGGGTC
TTGCCATGTTGTCCAGGCTGGTCTTGAACTCCTGACCTCACACAATCCTCCTGCCTTAGCCTCCTGAGTAACTGAGATTACAGGCACAAG
CTGCTGCACCCAGCTCTGTAGGTGACTTTTAAATGATTATACAATGGAAATAACATTCATTGACATTTCTGTGGTTTGAATCCAGAGAGA
TACTTCTTATAGAAAAACAAATGTTTATGCTAAAAATAACACCAAAATGTGGTGAACTCTTAAGGACTTTTCCCTTCAAGTGTGAAGGAA
GGTGTGATGAATGCTGTGGAGAGGCATCTGGAACAGAAATTCAAAATAAAGCCTTGACATTAAATACCCCTTCCACTGCTCACTTTGTGG
ATGGTAGCATGAGCTGTCTACCAAGAAGAAACCTGCTGCTCTCTTAATTTTAATATTTCCTAATTTGTTGATGGCCTTTTGTGTTGTGAA
CCACAACAAAGAGAGGCCTCTTTTGTGGCTGGTTATTCCAGTTCCCTGGGATTTTAAATTCTTTGGTCTATTAAGTATCCTTGTATTGGA
TACGTAATACCTTAGTGCTGTCATAATGTTGTACAAGATCATGATCAGCTTCTCCCTTTCTTCATTTTCTGTGATTTAACCATGTTCTTT
CCTGTCTCTTTCCATTTAAGATATTTTATTTGAATACTGATAAACATTTTATCCTGATAAGGAAGAATGTTCTTGTTACTTGATATACCT
CTGTCTTCATTCTCTTACAGCTTATCTTTCCTTAGGTTGATGGTGCCTCATTTAATAAGTAGATCTCTACTTAAGCTAAAGGATTATGAT
CATATGGTGTTGGAGACTATTAGCTATTAGATTTTTCTCAAAATATGAGTTTTGTACAATGGAATGAAAAAGTGACTTCATATACGTAAG
ACTGGTGCCTAAGAATGACTTGAATGTTATCAGTACTACCACAGAACTATAATATACATTGCCTTTTCTCAGCAGCATTTGAATTCAACT
TATTTATTTCCTCTATTTGTAGCTTTTTGTTCTAGAGTCAGAACAGGTGGACTTTGCTTTGAATATTTTAGATGGGACAAAAATTGTTCA
TCTTCGGAATTTATTTAAAGAAAGATCTACTGGTACAGGGTGTAAAAATACTTACAAGAATATGATTTTTTTGTTTGTTCATGCAAACAG
ATCCTTTACCCAATATATATTCTGGAGGTAGGTATACTGGTGCAACCCATTTGCCAGCTGTGTGACTTTGAGTAAGTTCTTAATTTCTCA
GAGTTTCAGTGTTCTCATCTATAAAATTATGTCTCCAATTGTGAGTACCTAGCACTGTACCTGATACATAGTAAACTCACAATAAATATT
TCTTAAACATAGATTTTGTTAGGAATACAGGCTTGATAACACAGTCCTTACCCTTACAATGTAATGTTAGAGATAAAGGAGCAAATAGAG
TCACGCATCACTTAATGACAGAGATATGTTCTGAGAAATGCATCGTTAGGTGATATTGTCATTGTGTAAACATCATGTTGTTTACTTCCA
CAAACCTAGATGGTATAGCCTACCAGTACACCTAGGCTATATGGTATAGTCTGTTGCTCCTAGGCTACAAACCCGTATAGCATGTTACTG
TCCTGCATGCTATAGACAGTTGGAACATGTAGACTTGTATAAACACTGCACACTTAACCTATACTAAATTTATTGAAAATATTTTTCTTG
GCCAGGCGTAGTGGCTCATGCCTGTAATCCTAACACTTCGGGAGGCCTAGGCGGGCAGGTTGTCTGAGCTCAGGAGTTTAGAGACCAGCC
CGGGCAACATGGCAAAACCCCATCTCTACTAAAAAAAATACCAAAAAAAGTTAGCCGGGTGTGGTGGCATAGTCCCGGCTACTCAGGAGT
CTGAGGCAGGAGAATAGCTTGAACCTGTGAGGCGGAGGTTGCAGTAAGCCGAGATCGCGCTACTGCACTCCAGCCTGGGCGACAGGGCAA
GACTGTCTTGAAAATAAACACACACACACACATTATATATATTATGTATTATATATATTAGATATATATTTTTTCTTCAATAAATTAACC
TTAGCTTACTGTAACTTTTACTTTATGAGTTTTTTTGTTTTGTTTTGTTTTTTTTTTTTGAGAAACAGGGTCTGTTACCTAATTTGGAGT
TTAGTGGCACAATCATGGCCCACTGCAGCCTTGACCTCTTAGACTCAAGTGATCCTCAAACCTCAGCCTCCCGAGTAGCTGGTACTATAG
GTGCGGGCCACCAACACTCAGCTAATTTTTAAATTTTGTAGAGACGAGGTCTTGCTTGTTGTCCAGTTGGGTTTTTTAGACTTTTTTTTT
TTTTTTTTTTTGGAGATGGAGTCTTGCTCTGTTGCCCAGGCTGGAGTGCAGTGGCATGATCTCGGCTCACTGCAACCTCTGACTCCTGGG
TTCAAGTGATTCTCCTGCCTCAGCCTCCCGAGTTGCTGGGACTACAGGCATGCGCCACCACACCCAGCTAATTTTTGTATTTTTAGTGAC
AGGGTTTCACCATGTTGGCCAGGCTGGTCTCAAACTCCTGACCTGGAGTGAGCCACTATATCTGGCCTTTATAAACTTTTTTGATTCTTG
TCATAACACTTAGCCTAAAATGCAAATGTACAGCTGTAGAAAAATACTTTATTTCTTTATATCCTTATTCTAGAAGCTTTTTTTCTATTA
ATTTTTGTTTGTTTGTTTTGTTTTACTATTTACTTCTAAAACTTTTTTGTTAAAAACCATGGCACAAACACACACATTATGCTAGGCATA
CAAAAGGTCAGGATCATCAGTCTCACTGTCTTCCACCCCCACTTCTCATCCCACCATTGTATCTGCTGTCTGTGGCTGACCAAAACATCA
TCATGTAGCACATGACTAGTGTGGCAAGTGCTCTGTTAGATGTAAGGCCATGATGCTAAAGCATCACAAGAGGGCATCTAACCCAGATTG
GGGATGTCATGGAAGGCCGACATCCTGAGTTGAATCCTGCAAATGTAAAAACCAATAGGCAAAGAAGAGGAACAAAAAGGATTCCAGGAC
AAACTGAGGTCACATCTATGATCCTTGACTTTATTGTGTCTGTTTAAAGTATCTACAGTAACCTGTATCAACTTAGTCAGTGTATTAATA
CTAAATTTAGCTCCTTCAAAGCAGTTGGAACTATGTGCTACATAAATTTCAGCTTCACACAAGGAAGGGAAGGAGTGAAATTAGTGAACA
GGCAGTTACAGCAAAAGAAAAAACATAAAAATTGAATAGCTGGCTCTGGTGAAATGAGCAAGGACTTTAGAGTCAAACTGGCCTGGATTT
GAATCCTGATCCTCATTGCTTGTAGCTGTATGATCTGGACAAATGACAGTAGCTGTTTCTAACCTTGATTTTCTCATCTGTAAGATGCCA
ATTGTAACTCCTAAGGATACTGAGGATTTTTTAAAATGCGTGTACAGTTCCTGACCAGTGGTTTGTGCCTAATAACTTATTACAAATTAT
TACCCAGTAAAAACCTTGAGACAAGAGTGAAAACGTAAAGCTAATTAATCCATTACTTGTTAGCAAGCAAACTACGTGCTTGAGAAAATT
ACTCAACTTTCATGTTTTACTTCCAGACAGTAGTTTGATTAAAAGAAAAAAAAAAATCACAGCCCAGGCATGTGGCTCACACCCTGGCAC
TTGGAGGCCGAGGTGGGAGCATAGCTTGAGCCTAGCAGTTTGAAACTAGCCTGGGCAACATAGATCCTATCTCTACAAAAAAAAAAAAAA
AAAAAAAAAAATTGGCCCGGTGTGGTGGTGCATGCCTGCGGTCCCAGCTACTCAGGAGGCTGAGGCGAGAGGATCGCTTGAGCCAGGGAG
GCTGAGGCTGCTGTGAGCCATGATCATGCCACTGCACTCCAGCCTGGACAACAGAGCAAGACCCTGTCTCAAAAAAAAAAAAAAATCAGT
AAAAAATATGCCGAAATAGCTTCAGAGTAATTATGAATTGCCTTCCAAAAACCACTGTTTTGCACTTGCCTGCAAGAATAGGAAAAACAA
AAGGACTGTAAGGTGGCAGGATGGCAGAACCAAACAAGAGTAGTACACCTTGGAGTTTTTTCGAAATATGGGTTGGGTTTTTGGGCTCTT
GGTTGATTTAAAATAAAATTTAAATGCCTTCAGCGTCTGTGATAGGTGCCAACATGAAGCTACCGAGTTCCAGAGAAAGAGGGGAGAGCT

>In-frame_ENST00000423018_ENST00000349699_TCGA-BR-8284-01A_PUM1_chr1_31532051_-_DDX52_chr17_35974398_length(amino acids)=135AA_start in transcript=89_stop in transcript=496
MSVACVLKRKAVLWQDSFSPHLKHHPQEPANPNMPVVLTSGTGSQAQPQPAANQALAAGTHSSPVPGSIGVAGRSQDDAMVDYFFQRQHG

--------------------------------------------------------------
>In-frame_ENST00000423018_ENST00000394367_TCGA-BR-8284-01A_PUM1_chr1_31532051_-_DDX52_chr17_35974398_length(transcript)=2438nt_BP=452nt
GAAAGGTAAGGGGGAGCGAGAGCGCCAGAGAGAGAAGATCGGGGGGCTGAAATCCATCTTCATCCTACCGCTCCGCCCGTGTTGGTGGAA
TGAGCGTTGCATGTGTCTTGAAGAGAAAAGCAGTGCTTTGGCAGGACTCTTTCAGCCCCCACCTGAAACATCACCCTCAAGAACCAGCTA
ATCCCAACATGCCTGTTGTTTTGACATCTGGAACAGGGTCGCAAGCGCAGCCACAACCAGCTGCAAATCAGGCTCTTGCAGCTGGGACTC
ACTCCAGCCCTGTCCCAGGATCTATAGGAGTTGCAGGCCGTTCCCAGGACGACGCTATGGTGGACTACTTCTTTCAGAGGCAGCATGGTG
AGCAGCTTGGGGGAGGAGGAAGTGGAGGAGGCGGCTATAATAATAGCAAACATCGATGGCCTACTGGGGATAACATTCATGCAGAACATC
AGGAAAAAGGTCACTGGTCAGAACAGCAAGAAGAAAGTAGCTCTTGAAGACAAAAGTTAAAAACAGACTTTAAAAATACTGTCCCAGAAA
TGTAATTTTATGATCCCAGCATGAATGTTATTTTCATGGAATACTTGAAGTCTTACAGTCACCTGTACCAAACATTTGAAATCAACTACA
AGTACATGGGACTGGTGATAAATGATCCTAAACTATCAAGTCAGTTTCAATTTGTAGGTGCCTTTTTTTTTTCCTGTAGAGATGAGGGTC
TTGCCATGTTGTCCAGGCTGGTCTTGAACTCCTGACCTCACACAATCCTCCTGCCTTAGCCTCCTGAGTAACTGAGATTACAGGCACAAG
CTGCTGCACCCAGCTCTGTAGGTGACTTTTAAATGATTATACAATGGAAATAACATTCATTGACATTTCTGTGGTTTGAATCCAGAGAGA
TACTTCTTATAGAAAAACAAATGTTTATGCTAAAAATAACACCAAAATGTGGTGAACTCTTAAGGACTTTTCCCTTCAAGTGTGAAGGAA
GGTGTGATGAATGCTGTGGAGAGGCATCTGGAACAGAAATTCAAAATAAAGCCTTGACATTAAATACCCCTTCCACTGCTCACTTTGTGG
ATGGTAGCATGAGCTGTCTACCAAGAAGAAACCTGCTGCTCTCTTAATTTTAATATTTCCTAATTTGTTGATGGCCTTTTGTGTTGTGAA
CCACAACAAAGAGAGGCCTCTTTTGTGGCTGGTTATTCCAGTTCCCTGGGATTTTAAATTCTTTGGTCTATTAAGTATCCTTGTATTGGA
TACGTAATACCTTAGTGCTGTCATAATGTTGTACAAGATCATGATCAGCTTCTCCCTTTCTTCATTTTCTGTGATTTAACCATGTTCTTT
CCTGTCTCTTTCCATTTAAGATATTTTATTTGAATACTGATAAACATTTTATCCTGATAAGGAAGAATGTTCTTGTTACTTGATATACCT
CTGTCTTCATTCTCTTACAGCTTATCTTTCCTTAGGTTGATGGTGCCTCATTTAATAAGTAGATCTCTACTTAAGCTAAAGGATTATGAT
CATATGGTGTTGGAGACTATTAGCTATTAGATTTTTCTCAAAATATGAGTTTTGTACAATGGAATGAAAAAGTGACTTCATATACGTAAG
ACTGGTGCCTAAGAATGACTTGAATGTTATCAGTACTACCACAGAACTATAATATACATTGCCTTTTCTCAGCAGCATTTGAATTCAACT
TATTTATTTCCTCTATTTGTAGCTTTTTGTTCTAGAGTCAGAACAGGTGGACTTTGCTTTGAATATTTTAGATGGGACAAAAATTGTTCA
TCTTCGGAATTTATTTAAAGAAAGATCTACTGGTACAGGGTGTAAAAATACTTACAAGAATATGATTTTTTTGTTTGTTCATGCAAACAG
ATCCTTTACCCAATATATATTCTGGAGGTAGGTATACTGGTGCAACCCATTTGCCAGCTGTGTGACTTTGAGTAAGTTCTTAATTTCTCA
GAGTTTCAGTGTTCTCATCTATAAAATTATGTCTCCAATTGTGAGTACCTAGCACTGTACCTGATACATAGTAAACTCACAATAAATATT
TCTTAAACATAGATTTTGTTAGGAATACAGGCTTGATAACACAGTCCTTACCCTTACAATGTAATGTTAGAGATAAAGGAGCAAATAGAG
TCACGCATCACTTAATGACAGAGATATGTTCTGAGAAATGCATCGTTAGGTGATATTGTCATTGTGTAAACATCATGTTGTTTACTTCCA
CAAACCTAGATGGTATAGCCTACCAGTACACCTAGGCTATATGGTATAGTCTGTTGCTCCTAGGCTACAAACCCGTATAGCATGTTACTG
TCCTGCATGCTATAGACAGTTGGAACATGTAGACTTGTATAAACACTGCACACTTAACCTATACTAAATTTATTGAAAATATTTTTCTTG

>In-frame_ENST00000423018_ENST00000394367_TCGA-BR-8284-01A_PUM1_chr1_31532051_-_DDX52_chr17_35974398_length(amino acids)=135AA_start in transcript=89_stop in transcript=496
MSVACVLKRKAVLWQDSFSPHLKHHPQEPANPNMPVVLTSGTGSQAQPQPAANQALAAGTHSSPVPGSIGVAGRSQDDAMVDYFFQRQHG

--------------------------------------------------------------

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Fusion Gene PPI Analysis for PUM1-DDX52


check button Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in

ChiPPI page.


check button Protein-protein interactors with each fusion partner protein in wild-type (BIOGRID-3.4.160)
HgeneHgene's interactorsTgeneTgene's interactors


check button - Retained PPIs in in-frame fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenStill interaction with


check button - Lost PPIs in in-frame fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenInteraction lost with


check button - Retained PPIs, but lost function due to frame-shift fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenInteraction lost with


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Related Drugs for PUM1-DDX52


check button Drugs targeting genes involved in this fusion gene.
(DrugBank Version 5.1.8 2021-05-08)
PartnerGeneUniProtAccDrugBank IDDrug nameDrug activityDrug typeDrug status

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Related Diseases for PUM1-DDX52


check button Diseases associated with fusion partners.
(DisGeNet 4.0)
PartnerGeneDisease IDDisease name# pubmedsSource
HgenePUM1C0004134Ataxia1GENOMICS_ENGLAND
HgenePUM1C0010417Cryptorchidism1GENOMICS_ENGLAND
HgenePUM1C0019193Hepatitis, Toxic1CTD_human
HgenePUM1C0036572Seizures1GENOMICS_ENGLAND
HgenePUM1C0557874Global developmental delay1GENOMICS_ENGLAND
HgenePUM1C0860207Drug-Induced Liver Disease1CTD_human
HgenePUM1C1262760Hepatitis, Drug-Induced1CTD_human
HgenePUM1C3658290Drug-Induced Acute Liver Injury1CTD_human
HgenePUM1C3714756Intellectual Disability1GENOMICS_ENGLAND
HgenePUM1C4025871Abnormality of the face1GENOMICS_ENGLAND
HgenePUM1C4277682Chemical and Drug Induced Liver Injury1CTD_human
HgenePUM1C4279912Chemically-Induced Liver Toxicity1CTD_human
HgenePUM1C4693672SPINOCEREBELLAR ATAXIA 471GENOMICS_ENGLAND;UNIPROT