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 | Fusion Gene Summary |
| Fusion Gene ORF analysis |
| Fusion Genomic Features |
| Fusion Protein Features |
| Fusion Gene Sequence |
| Fusion Gene PPI analysis |
| Related Drugs |
| Related Diseases |
Fusion gene:ASRGL1-STX5 (FusionGDB2 ID:7339) |
Fusion Gene Summary for ASRGL1-STX5 |
 Fusion gene summary |
| Fusion gene information | Fusion gene name: ASRGL1-STX5 | Fusion gene ID: 7339 | Hgene | Tgene | Gene symbol | ASRGL1 | STX5 | Gene ID | 80150 | 6811 |
| Gene name | asparaginase and isoaspartyl peptidase 1 | syntaxin 5 | |
| Synonyms | ALP|ALP1|CRASH | SED5|STX5A | |
| Cytomap | 11q12.3 | 11q12.3 | |
| Type of gene | protein-coding | protein-coding | |
| Description | isoaspartyl peptidase/L-asparaginaseL-asparaginaseL-asparagine amidohydrolaseasparaginase like 1asparaginase-like 1 proteinasparaginase-like protein 1beta-aspartyl-peptidaseisoaspartyl dipeptidasetestis secretory sperm-binding protein Li 242mP | syntaxin-5syntaxin 5A | |
| Modification date | 20200313 | 20200313 | |
| UniProtAcc | Q7L266 | . | |
| Ensembl transtripts involved in fusion gene | ENST00000415229, ENST00000535727, ENST00000301776, ENST00000528206, | ENST00000377897, ENST00000294179, ENST00000394690, ENST00000541317, | |
| Fusion gene scores | * DoF score | 16 X 12 X 4=768 | 6 X 2 X 7=84 |
| # samples | 19 | 9 | |
| ** MAII score | log2(19/768*10)=-2.01510689239021 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0 | log2(9/84*10)=0.0995356735509144 effective Gene in Pan-Cancer Fusion Genes (eGinPCFGs). DoF>8 and MAII>0 | |
| Context | PubMed: ASRGL1 [Title/Abstract] AND STX5 [Title/Abstract] AND fusion [Title/Abstract] | ||
| Most frequent breakpoint | ASRGL1(62105639)-STX5(62575100), # samples:1 | ||
| Anticipated loss of major functional domain due to fusion event. | ASRGL1-STX5 seems lost the major protein functional domain in Hgene partner, which is a essential gene due to the frame-shifted ORF. ASRGL1-STX5 seems lost the major protein functional domain in Tgene partner, which is a essential gene due to the frame-shifted ORF. | ||
| * DoF score (Degree of Frequency) = # partners X # break points X # cancer types ** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10) |
| Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez |
| Partner | Gene | GO ID | GO term | PubMed ID |
| Hgene | ASRGL1 | GO:0033345 | asparagine catabolic process via L-aspartate | 19839645 |
| Tgene | STX5 | GO:0042147 | retrograde transport, endosome to Golgi | 15215310 |
| Tgene | STX5 | GO:0090166 | Golgi disassembly | 16081076 |
| Tgene | STX5 | GO:1903358 | regulation of Golgi organization | 16081076 |
| Fusion gene breakpoints across ASRGL1 (5'-gene) * Click on the image to open the UCSC genome browser with custom track showing this image in a new window. |
| Fusion gene breakpoints across STX5 (3'-gene) * Click on the image to open the UCSC genome browser with custom track showing this image in a new window. |
| Fusion gene information from two resources (ChiTars 5.0 and ChimerDB 4.0) * All genome coordinats were lifted-over on hg19. * Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser. |
| Source | Disease | Sample | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand |
| ChimerDB4 | STAD | TCGA-HU-A4GN-01A | ASRGL1 | chr11 | 62105639 | + | STX5 | chr11 | 62575100 | - |
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Fusion Gene ORF analysis for ASRGL1-STX5 |
| Open reading frame (ORF) analsis of fusion genes based on Ensembl gene isoform structure. * Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser. |
| ORF | Henst | Tenst | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand |
| Frame-shift | ENST00000415229 | ENST00000377897 | ASRGL1 | chr11 | 62105639 | + | STX5 | chr11 | 62575100 | - |
| In-frame | ENST00000415229 | ENST00000294179 | ASRGL1 | chr11 | 62105639 | + | STX5 | chr11 | 62575100 | - |
| Frame-shift | ENST00000415229 | ENST00000394690 | ASRGL1 | chr11 | 62105639 | + | STX5 | chr11 | 62575100 | - |
| Frame-shift | ENST00000415229 | ENST00000541317 | ASRGL1 | chr11 | 62105639 | + | STX5 | chr11 | 62575100 | - |
| 5UTR-3CDS | ENST00000535727 | ENST00000377897 | ASRGL1 | chr11 | 62105639 | + | STX5 | chr11 | 62575100 | - |
| 5UTR-3CDS | ENST00000535727 | ENST00000294179 | ASRGL1 | chr11 | 62105639 | + | STX5 | chr11 | 62575100 | - |
| 5UTR-3CDS | ENST00000535727 | ENST00000394690 | ASRGL1 | chr11 | 62105639 | + | STX5 | chr11 | 62575100 | - |
| 5UTR-3CDS | ENST00000535727 | ENST00000541317 | ASRGL1 | chr11 | 62105639 | + | STX5 | chr11 | 62575100 | - |
| Frame-shift | ENST00000301776 | ENST00000377897 | ASRGL1 | chr11 | 62105639 | + | STX5 | chr11 | 62575100 | - |
| In-frame | ENST00000301776 | ENST00000294179 | ASRGL1 | chr11 | 62105639 | + | STX5 | chr11 | 62575100 | - |
| Frame-shift | ENST00000301776 | ENST00000394690 | ASRGL1 | chr11 | 62105639 | + | STX5 | chr11 | 62575100 | - |
| Frame-shift | ENST00000301776 | ENST00000541317 | ASRGL1 | chr11 | 62105639 | + | STX5 | chr11 | 62575100 | - |
| intron-3CDS | ENST00000528206 | ENST00000377897 | ASRGL1 | chr11 | 62105639 | + | STX5 | chr11 | 62575100 | - |
| intron-3CDS | ENST00000528206 | ENST00000294179 | ASRGL1 | chr11 | 62105639 | + | STX5 | chr11 | 62575100 | - |
| intron-3CDS | ENST00000528206 | ENST00000394690 | ASRGL1 | chr11 | 62105639 | + | STX5 | chr11 | 62575100 | - |
| intron-3CDS | ENST00000528206 | ENST00000541317 | ASRGL1 | chr11 | 62105639 | + | STX5 | chr11 | 62575100 | - |
| ORFfinder result based on the fusion transcript sequence of in-frame fusion genes. |
| Henst | Tenst | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand | Seq length (transcript) | BP loci (transcript) | Predicted start (transcript) | Predicted stop (transcript) | Seq length (amino acids) |
| ENST00000415229 | ASRGL1 | chr11 | 62105639 | + | ENST00000294179 | STX5 | chr11 | 62575100 | - | 1137 | 405 | 16 | 564 | 182 |
| ENST00000301776 | ASRGL1 | chr11 | 62105639 | + | ENST00000294179 | STX5 | chr11 | 62575100 | - | 1055 | 323 | 375 | 1 | 125 |
| DeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated. |
| Henst | Tenst | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand | No-coding score | Coding score |
| ENST00000415229 | ENST00000294179 | ASRGL1 | chr11 | 62105639 | + | STX5 | chr11 | 62575100 | - | 0.14169799 | 0.85830206 |
| ENST00000301776 | ENST00000294179 | ASRGL1 | chr11 | 62105639 | + | STX5 | chr11 | 62575100 | - | 0.08304242 | 0.9169575 |
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Fusion Genomic Features for ASRGL1-STX5 |
| FusionAI prediction of the potential fusion gene breakpoint based on the pre-mature RNA sequence context (+/- 5kb of individual partner genes, total 20kb length sequence). FusionAI is a fusion gene breakpoint classifier based on convolutional neural network by comparing the fusion positive and negative sequence context of ~ 20K fusion gene data. From here, we can have the relative potentency of the 20K genomic sequence how individual sequnce will be likely used as the gene fusion breakpoints. |
| Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand | 1-p | p (fusion gene breakpoint) |
| Distribution of 44 human genomic features loci across 20kb length fusion breakpoint regions. We integrated a total of 44 different types of human genomic feature loci information across five big categories including virus integration sites, repeats, structural variants, chromatin states, and gene expression regulation. More details are in help page. |
| Distribution of 44 human genomic features loci across 20kb length fusion breakpoint regions that are ovelapped with the top 1% feature importance score regions. More details are in help page. |
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Fusion Protein Features for ASRGL1-STX5 |
| Go to FGviewer for the breakpoints of chr11:62105639-chr11:62575100 - FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels. |
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| Main function of each fusion partner protein. (from UniProt) |
| Hgene | Tgene |
| ASRGL1 | . |
| FUNCTION: Has both L-asparaginase and beta-aspartyl peptidase activity. May be involved in the production of L-aspartate, which can act as an excitatory neurotransmitter in some brain regions. Is highly active with L-Asp beta-methyl ester. Besides, has catalytic activity toward beta-aspartyl dipeptides and their methyl esters, including beta-L-Asp-L-Phe, beta-L-Asp-L-Phe methyl ester (aspartame), beta-L-Asp-L-Ala, beta-L-Asp-L-Leu and beta-L-Asp-L-Lys. Does not have aspartylglucosaminidase activity and is inactive toward GlcNAc-L-Asn. Likewise, has no activity toward glutamine. {ECO:0000269|PubMed:19839645, ECO:0000269|PubMed:27106100}. | FUNCTION: Might normally function as a transcriptional repressor. EWS-fusion-proteins (EFPS) may play a role in the tumorigenic process. They may disturb gene expression by mimicking, or interfering with the normal function of CTD-POLII within the transcription initiation complex. They may also contribute to an aberrant activation of the fusion protein target genes. |
| Retention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at * Minus value of BPloci means that the break pointn is located before the CDS. |
| - In-frame and retained protein feature among the 13 regional features. |
| Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Protein feature | Protein feature note |
| Tgene | STX5 | chr11:62105639 | chr11:62575100 | ENST00000394690 | 9 | 11 | 287_318 | 248.66666666666666 | 302.0 | Coiled coil | Ontology_term=ECO:0000255 | |
| Tgene | STX5 | chr11:62105639 | chr11:62575100 | ENST00000394690 | 9 | 11 | 263_325 | 248.66666666666666 | 302.0 | Domain | t-SNARE coiled-coil homology | |
| Tgene | STX5 | chr11:62105639 | chr11:62575100 | ENST00000294179 | 9 | 11 | 355_355 | 302.6666666666667 | 356.0 | Topological domain | Vesicular | |
| Tgene | STX5 | chr11:62105639 | chr11:62575100 | ENST00000394690 | 9 | 11 | 355_355 | 248.66666666666666 | 302.0 | Topological domain | Vesicular | |
| Tgene | STX5 | chr11:62105639 | chr11:62575100 | ENST00000294179 | 9 | 11 | 334_354 | 302.6666666666667 | 356.0 | Transmembrane | Helical%3B Anchor for type IV membrane protein | |
| Tgene | STX5 | chr11:62105639 | chr11:62575100 | ENST00000394690 | 9 | 11 | 334_354 | 248.66666666666666 | 302.0 | Transmembrane | Helical%3B Anchor for type IV membrane protein |
| - In-frame and not-retained protein feature among the 13 regional features. |
| Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Protein feature | Protein feature note |
| Hgene | ASRGL1 | chr11:62105639 | chr11:62575100 | ENST00000301776 | + | 2 | 7 | 196_199 | 63.333333333333336 | 309.0 | Region | Note=Substrate binding |
| Hgene | ASRGL1 | chr11:62105639 | chr11:62575100 | ENST00000301776 | + | 2 | 7 | 219_222 | 63.333333333333336 | 309.0 | Region | Note=Substrate binding |
| Hgene | ASRGL1 | chr11:62105639 | chr11:62575100 | ENST00000415229 | + | 2 | 7 | 196_199 | 63.333333333333336 | 309.0 | Region | Note=Substrate binding |
| Hgene | ASRGL1 | chr11:62105639 | chr11:62575100 | ENST00000415229 | + | 2 | 7 | 219_222 | 63.333333333333336 | 309.0 | Region | Note=Substrate binding |
| Tgene | STX5 | chr11:62105639 | chr11:62575100 | ENST00000294179 | 9 | 11 | 287_318 | 302.6666666666667 | 356.0 | Coiled coil | Ontology_term=ECO:0000255 | |
| Tgene | STX5 | chr11:62105639 | chr11:62575100 | ENST00000294179 | 9 | 11 | 263_325 | 302.6666666666667 | 356.0 | Domain | t-SNARE coiled-coil homology | |
| Tgene | STX5 | chr11:62105639 | chr11:62575100 | ENST00000294179 | 9 | 11 | 245_247 | 302.6666666666667 | 356.0 | Motif | IxM motif%3B signal for cargo packaging into COPII-coated vesicles | |
| Tgene | STX5 | chr11:62105639 | chr11:62575100 | ENST00000394690 | 9 | 11 | 245_247 | 248.66666666666666 | 302.0 | Motif | IxM motif%3B signal for cargo packaging into COPII-coated vesicles | |
| Tgene | STX5 | chr11:62105639 | chr11:62575100 | ENST00000294179 | 9 | 11 | 1_333 | 302.6666666666667 | 356.0 | Topological domain | Cytoplasmic | |
| Tgene | STX5 | chr11:62105639 | chr11:62575100 | ENST00000394690 | 9 | 11 | 1_333 | 248.66666666666666 | 302.0 | Topological domain | Cytoplasmic |
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Fusion Gene Sequence for ASRGL1-STX5 |
| For in-frame fusion transcripts, we provide the fusion transcript sequences and fusion amino acid sequences. To have fusion amino acid sequence, we ran ORFfinder and chose the longest ORF among the all predicted ones. |
| >In-frame_ENST00000415229_ENST00000294179_TCGA-HU-A4GN-01A_ASRGL1_chr11_62105639_+_STX5_chr11_62575100_length(transcript)=1137nt_BP=405nt GTTGTGACCAGAGCGGTTGCGGGCTGAGCGGTTTCGAGCCGGCGTCGGGGAGCGGCGGTACCGGGCGGCTGCGGGGCTGGCTCGACCCAG CTTGAGGTCTCGGCGTCCGCGTCCTGCGGTGCCCTGGGGTCTCCCGAGGACCTTGTACCCGCGCGGCTTCCTTGGGCTGGCTTTGGACGA CGCTTTCGCCTTCCTGCTGCCTAGGATCCGCCGACATGAATCCCATCGTAGTGGTCCACGGCGGCGGAGCCGGTCCCATCTCCAAGGATC GGAAGGAGCGAGTGCACCAGGGCATGGTCAGAGCCGCCACCGTGGGCTACGGCATCCTCCGGGAGGGCGGGAGCGCCGTGGATGCCGTAG AGGGAGCTGTCGTCGCCCTGGAAGACGATCCCGAGTTCAACGCAGGATCGACGAGAACGTGCTAGGAGCCCAGCTGGACGTTGAGGCCGC CCATTCAGAGATCCTCAAGTACTTCCAGTCTGTCACCTCCAACCGGTGGCTCATGGTCAAAATCTTCCTCATCCTCATTGTCTTCTTCAT CATCTTTGTGGTCTTCCTTGCTTGAACCCTCTCTACTCTGAGGCACTCTGTTGGGGTTTGGGACCCTCCTGGGAAGGCAAGTGGCCAGTG CTGCCACTGAGCCTGTGCAGGGTACTTGGGAGAAAGGCCCTGTTTCCCTGGAACTGCTAAGAATGACCACTGCCCCTGATCCCCCACCCC TTGCCTCTGGCCACCCTGTCCTCCCCCCACCACCCTCAGGCCTATGAAACACACAGGGTTCTAGATTTGAACTCTGCTGTGAAGTGACTG GAAGGGAGCAGAGGCCAGCTGGGGGCCAGTGGGGGAGGTTGTTTCCACTAGGAGATTTTTATAAACCCTCTCCAGCCTCTCCCAAAGGAA GCGTTGGCAGCAAAGGGAGATGATGCCCTTACCCACCTTCCTGTGAGTGAAGAGAGGAAGCAGCCCCAGGGACCAATTTTCCCAATTGAC CTCTTTCTTCCTCTTTCACCATGTGAGGCAGGGAGCCCTGAGCCCTTCAGCTGCCTGCACAACCCCTGACATTGGCTGCTGGTGACTCAA >In-frame_ENST00000415229_ENST00000294179_TCGA-HU-A4GN-01A_ASRGL1_chr11_62105639_+_STX5_chr11_62575100_length(amino acids)=182AA_start in transcript=16_stop in transcript=564 MRAERFRAGVGERRYRAAAGLARPSLRSRRPRPAVPWGLPRTLYPRGFLGLALDDAFAFLLPRIRRHESHRSGPRRRSRSHLQGSEGASA PGHGQSRHRGLRHPPGGRERRGCRRGSCRRPGRRSRVQRRIDENVLGAQLDVEAAHSEILKYFQSVTSNRWLMVKIFLILIVFFIIFVVF -------------------------------------------------------------- >In-frame_ENST00000301776_ENST00000294179_TCGA-HU-A4GN-01A_ASRGL1_chr11_62105639_+_STX5_chr11_62575100_length(transcript)=1055nt_BP=323nt GACCAGAGCGGTTGCGGGCTGAGCGGTTTCGAGCCGGCGTCGGGGAGCGGCGGTACCGGGCGGCTGCGGGGCTGGCTCGACCCAGCTTGA GGTCTCGGCGTCCGCGTCCTGCGGTGCCCTGGGATCCGCCGACATGAATCCCATCGTAGTGGTCCACGGCGGCGGAGCCGGTCCCATCTC CAAGGATCGGAAGGAGCGAGTGCACCAGGGCATGGTCAGAGCCGCCACCGTGGGCTACGGCATCCTCCGGGAGGGCGGGAGCGCCGTGGA TGCCGTAGAGGGAGCTGTCGTCGCCCTGGAAGACGATCCCGAGTTCAACGCAGGATCGACGAGAACGTGCTAGGAGCCCAGCTGGACGTT GAGGCCGCCCATTCAGAGATCCTCAAGTACTTCCAGTCTGTCACCTCCAACCGGTGGCTCATGGTCAAAATCTTCCTCATCCTCATTGTC TTCTTCATCATCTTTGTGGTCTTCCTTGCTTGAACCCTCTCTACTCTGAGGCACTCTGTTGGGGTTTGGGACCCTCCTGGGAAGGCAAGT GGCCAGTGCTGCCACTGAGCCTGTGCAGGGTACTTGGGAGAAAGGCCCTGTTTCCCTGGAACTGCTAAGAATGACCACTGCCCCTGATCC CCCACCCCTTGCCTCTGGCCACCCTGTCCTCCCCCCACCACCCTCAGGCCTATGAAACACACAGGGTTCTAGATTTGAACTCTGCTGTGA AGTGACTGGAAGGGAGCAGAGGCCAGCTGGGGGCCAGTGGGGGAGGTTGTTTCCACTAGGAGATTTTTATAAACCCTCTCCAGCCTCTCC CAAAGGAAGCGTTGGCAGCAAAGGGAGATGATGCCCTTACCCACCTTCCTGTGAGTGAAGAGAGGAAGCAGCCCCAGGGACCAATTTTCC CAATTGACCTCTTTCTTCCTCTTTCACCATGTGAGGCAGGGAGCCCTGAGCCCTTCAGCTGCCTGCACAACCCCTGACATTGGCTGCTGG >In-frame_ENST00000301776_ENST00000294179_TCGA-HU-A4GN-01A_ASRGL1_chr11_62105639_+_STX5_chr11_62575100_length(amino acids)=125AA_start in transcript=375_stop in transcript=1 MNGRPQRPAGLLARSRRSCVELGIVFQGDDSSLYGIHGAPALPEDAVAHGGGSDHALVHSLLPILGDGTGSAAVDHYDGIHVGGSQGTAG -------------------------------------------------------------- |
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Fusion Gene PPI Analysis for ASRGL1-STX5 |
| Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in |
| Protein-protein interactors with each fusion partner protein in wild-type (BIOGRID-3.4.160) |
| Hgene | Hgene's interactors | Tgene | Tgene's interactors |
| - Retained PPIs in in-frame fusion. |
| Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Still interaction with |
| - Lost PPIs in in-frame fusion. |
| Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Interaction lost with |
| - Retained PPIs, but lost function due to frame-shift fusion. |
| Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Interaction lost with |
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Related Drugs for ASRGL1-STX5 |
| Drugs targeting genes involved in this fusion gene. (DrugBank Version 5.1.8 2021-05-08) |
| Partner | Gene | UniProtAcc | DrugBank ID | Drug name | Drug activity | Drug type | Drug status |
| Hgene | ASRGL1 | Q7L266 | DB00174 | Asparagine | Substrate | Small molecule | Approved|Investigational|Nutraceutical |
| Hgene | ASRGL1 | Q7L266 | DB00174 | Asparagine | Substrate | Small molecule | Approved|Investigational|Nutraceutical |
| Hgene | ASRGL1 | Q7L266 | DB00174 | Asparagine | Substrate | Small molecule | Approved|Investigational|Nutraceutical |
| Hgene | ASRGL1 | Q7L266 | DB00128 | Aspartic acid | Small molecule | Approved|Nutraceutical | |
| Hgene | ASRGL1 | Q7L266 | DB00128 | Aspartic acid | Small molecule | Approved|Nutraceutical | |
| Hgene | ASRGL1 | Q7L266 | DB00128 | Aspartic acid | Small molecule | Approved|Nutraceutical |
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Related Diseases for ASRGL1-STX5 |
| Diseases associated with fusion partners. (DisGeNet 4.0) |
| Partner | Gene | Disease ID | Disease name | # pubmeds | Source |
| Hgene | ASRGL1 | C0162820 | Dermatitis, Allergic Contact | 1 | CTD_human |
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