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Center for Computational Systems Medicine
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Fusion Gene Summary

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Fusion Gene ORF analysis

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Fusion Genomic Features

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Fusion Protein Features

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Fusion Gene Sequence

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Fusion Gene PPI analysis

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Related Drugs

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Related Diseases

Fusion gene:RNF149-MAP4K4 (FusionGDB2 ID:74367)

Fusion Gene Summary for RNF149-MAP4K4

check button Fusion gene summary
Fusion gene informationFusion gene name: RNF149-MAP4K4
Fusion gene ID: 74367
HgeneTgene
Gene symbol

RNF149

MAP4K4

Gene ID

284996

9448

Gene namering finger protein 149mitogen-activated protein kinase kinase kinase kinase 4
SynonymsDNAPTP2FLH21957|HEL-S-31|HGK|MEKKK4|NIK
Cytomap

2q11.2

2q11.2

Type of geneprotein-codingprotein-coding
DescriptionE3 ubiquitin-protein ligase RNF149DNA polymerase-transactivated protein 2RING-type E3 ubiquitin transferase RNF149mitogen-activated protein kinase kinase kinase kinase 4HPK/GCK-like kinase HGKMAPK/ERK kinase kinase kinase 4MEK kinase kinase 4Ste20 group protein kinase HGKepididymis secretory protein Li 31hepatocyte progenitor kinase-like/germinal center kinase-
Modification date2020031320200315
UniProtAcc.

O95819

Ensembl transtripts involved in fusion geneENST00000485752, ENST00000295317, 
ENST00000425019, ENST00000324219, 
ENST00000350198, ENST00000302217, 
ENST00000413150, ENST00000347699, 
ENST00000456652, ENST00000350878, 
ENST00000498066, 
Fusion gene scores* DoF score5 X 5 X 2=5013 X 14 X 8=1456
# samples 614
** MAII scorelog2(6/50*10)=0.263034405833794
effective Gene in Pan-Cancer Fusion Genes (eGinPCFGs).
DoF>8 and MAII>0		log2(14/1456*10)=-3.37851162325373
possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs).
DoF>8 and MAII<0
Context

PubMed: RNF149 [Title/Abstract] AND MAP4K4 [Title/Abstract] AND fusion [Title/Abstract]

Most frequent breakpointRNF149(101910476)-MAP4K4(102407182), # samples:2
Anticipated loss of major functional domain due to fusion event.
* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

check button Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez
PartnerGeneGO IDGO termPubMed ID
TgeneMAP4K4

GO:0006468

protein phosphorylation

9890973

TgeneMAP4K4

GO:0035556

intracellular signal transduction

9890973

TgeneMAP4K4

GO:0046328

regulation of JNK cascade

14966141


check buttonFusion gene breakpoints across RNF149 (5'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

check buttonFusion gene breakpoints across MAP4K4 (3'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

check button Fusion gene information from two resources (ChiTars 5.0 and ChimerDB 4.0)
* All genome coordinats were lifted-over on hg19.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.
SourceDiseaseSampleHgeneHchrHbpHstrandTgeneTchrTbpTstrand
ChimerDB4OVTCGA-24-1553-01ARNF149chr2

101910476

-MAP4K4chr2

102407182

+
ChimerDB4OVTCGA-24-1553-01ARNF149chr2

101910476

-MAP4K4chr2

102407182

+


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Fusion Gene ORF analysis for RNF149-MAP4K4

check button Open reading frame (ORF) analsis of fusion genes based on Ensembl gene isoform structure.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.
ORFHenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrand
intron-3CDSENST00000485752ENST00000425019RNF149chr2

101910476

-MAP4K4chr2

102407182

+
intron-3CDSENST00000485752ENST00000324219RNF149chr2

101910476

-MAP4K4chr2

102407182

+
intron-3CDSENST00000485752ENST00000350198RNF149chr2

101910476

-MAP4K4chr2

102407182

+
intron-3CDSENST00000485752ENST00000302217RNF149chr2

101910476

-MAP4K4chr2

102407182

+
intron-3CDSENST00000485752ENST00000413150RNF149chr2

101910476

-MAP4K4chr2

102407182

+
intron-3CDSENST00000485752ENST00000347699RNF149chr2

101910476

-MAP4K4chr2

102407182

+
intron-3CDSENST00000485752ENST00000456652RNF149chr2

101910476

-MAP4K4chr2

102407182

+
intron-intronENST00000485752ENST00000350878RNF149chr2

101910476

-MAP4K4chr2

102407182

+
intron-intronENST00000485752ENST00000498066RNF149chr2

101910476

-MAP4K4chr2

102407182

+
In-frameENST00000295317ENST00000425019RNF149chr2

101910476

-MAP4K4chr2

102407182

+
In-frameENST00000295317ENST00000324219RNF149chr2

101910476

-MAP4K4chr2

102407182

+
In-frameENST00000295317ENST00000350198RNF149chr2

101910476

-MAP4K4chr2

102407182

+
In-frameENST00000295317ENST00000302217RNF149chr2

101910476

-MAP4K4chr2

102407182

+
In-frameENST00000295317ENST00000413150RNF149chr2

101910476

-MAP4K4chr2

102407182

+
In-frameENST00000295317ENST00000347699RNF149chr2

101910476

-MAP4K4chr2

102407182

+
In-frameENST00000295317ENST00000456652RNF149chr2

101910476

-MAP4K4chr2

102407182

+
5CDS-intronENST00000295317ENST00000350878RNF149chr2

101910476

-MAP4K4chr2

102407182

+
5CDS-intronENST00000295317ENST00000498066RNF149chr2

101910476

-MAP4K4chr2

102407182

+

check buttonORFfinder result based on the fusion transcript sequence of in-frame fusion genes.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandSeq length
(transcript)		BP loci
(transcript)		Predicted start
(transcript)		Predicted stop
(transcript)		Seq length
(amino acids)
ENST00000295317RNF149chr2101910476-ENST00000425019MAP4K4chr2102407182+48318887545831502
ENST00000295317RNF149chr2101910476-ENST00000324219MAP4K4chr2102407182+48078887547271550
ENST00000295317RNF149chr2101910476-ENST00000350198MAP4K4chr2102407182+43458887542651396
ENST00000295317RNF149chr2101910476-ENST00000302217MAP4K4chr2102407182+39738887538931272
ENST00000295317RNF149chr2101910476-ENST00000413150MAP4K4chr2102407182+76688887542291384
ENST00000295317RNF149chr2101910476-ENST00000347699MAP4K4chr2102407182+45578887544841469
ENST00000295317RNF149chr2101910476-ENST00000456652MAP4K4chr2102407182+46208887538811268

check buttonDeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandNo-coding scoreCoding score
ENST00000295317ENST00000425019RNF149chr2101910476-MAP4K4chr2102407182+0.0006140470.99938595
ENST00000295317ENST00000324219RNF149chr2101910476-MAP4K4chr2102407182+0.0013535640.99864644
ENST00000295317ENST00000350198RNF149chr2101910476-MAP4K4chr2102407182+0.0011235910.9988764
ENST00000295317ENST00000302217RNF149chr2101910476-MAP4K4chr2102407182+0.0012148820.9987851
ENST00000295317ENST00000413150RNF149chr2101910476-MAP4K4chr2102407182+0.0001258390.9998741
ENST00000295317ENST00000347699RNF149chr2101910476-MAP4K4chr2102407182+0.0005712080.9994288
ENST00000295317ENST00000456652RNF149chr2101910476-MAP4K4chr2102407182+0.0002910590.99970895

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Fusion Genomic Features for RNF149-MAP4K4


check buttonFusionAI prediction of the potential fusion gene breakpoint based on the pre-mature RNA sequence context (+/- 5kb of individual partner genes, total 20kb length sequence). FusionAI is a fusion gene breakpoint classifier based on convolutional neural network by comparing the fusion positive and negative sequence context of ~ 20K fusion gene data. From here, we can have the relative potentency of the 20K genomic sequence how individual sequnce will be likely used as the gene fusion breakpoints.
HgeneHchrHbpHstrandTgeneTchrTbpTstrand1-pp (fusion gene breakpoint)
RNF149chr2101910475-MAP4K4chr2102407181+3.57E-081
RNF149chr2101910475-MAP4K4chr2102407181+3.57E-081

check buttonDistribution of 44 human genomic features loci across 20kb length fusion breakpoint regions. We integrated a total of 44 different types of human genomic feature loci information across five big categories including virus integration sites, repeats, structural variants, chromatin states, and gene expression regulation. More details are in help page.

check buttonDistribution of 44 human genomic features loci across 20kb length fusion breakpoint regions that are ovelapped with the top 1% feature importance score regions. More details are in help page.

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Fusion Protein Features for RNF149-MAP4K4


check button Go to

FGviewer for the breakpoints of chr2:101910476-chr2:102407182

.
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
FGviewer

check buttonMain function of each fusion partner protein. (from UniProt)
HgeneTgene
.MAP4K4

O95819

FUNCTION: Might normally function as a transcriptional repressor. EWS-fusion-proteins (EFPS) may play a role in the tumorigenic process. They may disturb gene expression by mimicking, or interfering with the normal function of CTD-POLII within the transcription initiation complex. They may also contribute to an aberrant activation of the fusion protein target genes.FUNCTION: Serine/threonine kinase that may play a role in the response to environmental stress and cytokines such as TNF-alpha. Appears to act upstream of the JUN N-terminal pathway. Phosphorylates SMAD1 on Thr-322. {ECO:0000269|PubMed:21690388, ECO:0000269|PubMed:9890973}.

check buttonRetention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at

download page


* Minus value of BPloci means that the break pointn is located before the CDS.
- In-frame and retained protein feature among the 13 regional features.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note
HgeneRNF149chr2:101910476chr2:102407182ENST00000295317-3767_175260.0401.0DomainNote=PA
HgeneRNF149chr2:101910476chr2:102407182ENST00000295317-37201_221260.0401.0TransmembraneHelical
TgeneMAP4K4chr2:101910476chr2:102407182ENST00000347699130926_121341.01240.0DomainCNH

- In-frame and not-retained protein feature among the 13 regional features.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note
HgeneRNF149chr2:101910476chr2:102407182ENST00000295317-37269_310260.0401.0Zinc fingerRING-type%3B atypical
TgeneMAP4K4chr2:101910476chr2:102407182ENST0000034769913025_28941.01240.0DomainProtein kinase
TgeneMAP4K4chr2:101910476chr2:102407182ENST0000034769913031_3941.01240.0Nucleotide bindingATP


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Fusion Gene Sequence for RNF149-MAP4K4


check button For in-frame fusion transcripts, we provide the fusion transcript sequences and fusion amino acid sequences. To have fusion amino acid sequence, we ran ORFfinder and chose the longest ORF among the all predicted ones.
>In-frame_ENST00000295317_ENST00000425019_TCGA-24-1553-01A_RNF149_chr2_101910476_-_MAP4K4_chr2_102407182_length(transcript)=4831nt_BP=888nt
CGCCTCAGTCTTCCAGGGCGGCGGTGGGTGTCCGCTTCTCTCTGCTCTTCGACTGCACCGCACTCGCGCGTGACCCTGACTCCCCCTAGT
CAGCTCAGCGGTGCTGCCATGGCGTGGCGGCGGCGCGAAGCCAGCGTCGGGGCTCGCGGCGTGTTGGCTCTGGCGTTGCTCGCCCTGGCC
CTGTGCGTGCCCGGGGCCCGGGGCCGGGCTCTCGAGTGGTTCTCGGCCGTGGTAAACATCGAGTACGTGGACCCGCAGACCAACCTGACG
GTGTGGAGCGTCTCGGAGAGTGGCCGCTTCGGCGACAGCTCGCCCAAGGAGGGCGCGCATGGCCTGGTGGGCGTCCCGTGGGCGCCCGGC
GGAGACCTCGAGGGCTGCGCGCCCGACACGCGCTTCTTCGTGCCCGAGCCCGGCGGCCGAGGGGCCGCGCCCTGGGTCGCCCTGGTGGCT
CGTGGGGGCTGCACCTTCAAGGACAAGGTGCTGGTGGCGGCGCGGAGGAACGCCTCGGCCGTCGTCCTCTACAATGAGGAGCGCTACGGG
AACATCACCTTGCCCATGTCTCACGCGGGAACAGGAAATATAGTGGTCATTATGATTAGCTATCCAAAAGGAAGAGAAATTTTGGAGCTG
GTGCAAAAAGGAATTCCAGTAACGATGACCATAGGGGTTGGCACCCGGCATGTACAGGAGTTCATCAGCGGTCAGTCTGTGGTGTTTGTG
GCCATTGCCTTCATCACCATGATGATTATCTCGTTAGCCTGGCTAATATTTTACTATATACAGCGTTTCCTATATACTGGCTCTCAGATT
GGAAGTCAGAGCCATAGAAAAGAAACTAAGAAAGTTATTGGCCAGCTTCTACTTCATACTGTAAAGCATGGAGAAAAGGGTCGACATGTT
AAAACGGGTCAGTTGGCAGCCATCAAAGTTATGGATGTCACTGAGGATGAAGAGGAAGAAATCAAACTGGAGATAAATATGCTAAAGAAA
TACTCTCATCACAGAAACATTGCAACATATTATGGTGCTTTCATCAAAAAGAGCCCTCCAGGACATGATGACCAACTCTGGCTTGTTATG
GAGTTCTGTGGGGCTGGGTCCATTACAGACCTTGTGAAGAACACCAAAGGGAACACACTCAAAGAAGACTGGATCGCTTACATCTCCAGA
GAAATCCTGAGGGGACTGGCACATCTTCACATTCATCATGTGATTCACCGGGATATCAAGGGCCAGAATGTGTTGCTGACTGAGAATGCA
GAGGTGAAACTTGTTGACTTTGGTGTGAGTGCTCAGCTGGACAGGACTGTGGGGCGGAGAAATACGTTCATAGGCACTCCCTACTGGATG
GCTCCTGAGGTCATCGCCTGTGATGAGAACCCAGATGCCACCTATGATTACAGAAGTGATCTTTGGTCTTGTGGCATTACAGCCATTGAG
ATGGCAGAAGGTGCTCCCCCTCTCTGTGACATGCATCCAATGAGAGCACTGTTTCTCATTCCCAGAAACCCTCCTCCCCGGCTGAAGTCA
AAAAAATGGTCGAAGAAGTTTTTTAGTTTTATAGAAGGGTGCCTGGTGAAGAATTACATGCAGCGGCCCTCTACAGAGCAGCTTTTGAAA
CATCCTTTTATAAGGGATCAGCCAAATGAAAGGCAAGTTAGAATCCAGCTTAAGGATCATATAGATCGTACCAGGAAGAAGAGAGGCGAG
AAAGATGAAACTGAGTATGAGTACAGTGGGAGTGAGGAAGAAGAGGAGGAAGTGCCTGAACAGGAAGGAGAGCCAAGTTCCATTGTGAAC
GTGCCTGGTGAGTCTACTCTTCGCCGAGATTTCCTGAGACTGCAGCAGGAGAACAAGGAACGTTCCGAGGCTCTTCGGAGACAACAGTTA
CTACAGGAGCAACAGCTCCGGGAGCAGGAAGAATATAAAAGGCAACTGCTGGCAGAGAGACAGAAGCGGATTGAGCAGCAGAAAGAACAG
AGGCGACGGCTAGAAGAGCAACAAAGGAGAGAGCGGGAAGCTAGAAGGCAGCAGGAACGTGAACAGCGAAGGAGAGAACAAGAAGAAAAG
AGGCGTCTAGAGGAGTTGGAGAGAAGGCGCAAAGAAGAAGAGGAGAGGAGACGGGCAGAAGAAGAAAAGAGGAGAGTTGAAAGAGAACAG
GAGTATATCAGGCGACAGCTAGAAGAGGAGCAGCGGCACTTGGAAGTCCTTCAGCAGCAGCTGCTCCAGGAGCAGGCCATGTTACTGCAT
GACCATAGGAGGCCGCACCCGCAGCACTCGCAGCAGCCGCCACCACCGCAGCAGGAAAGGAGCAAGCCAAGCTTCCATGCTCCCGAGCCC
AAAGCCCACTACGAGCCTGCTGACCGAGCGCGAGAGGTGGAAGATAGATTTAGGAAAACTAACCACAGCTCCCCTGAAGCCCAGTCTAAG
CAGACAGGCAGAGTATTGGAGCCACCAGTGCCTTCCCGATCAGAGTCTTTTTCCAATGGCAACTCCGAGTCTGTGCATCCCGCCCTGCAG
AGACCAGCGGAGCCACAGGTTCCTGTGAGAACAACATCTCGCTCCCCTGTTCTGTCCCGTCGAGATTCCCCACTGCAGGGCAGTGGGCAG
CAGAATAGCCAGGCAGGACAGAGAAACTCCACCAGTATTGAGCCCAGGCTTCTGTGGGAGAGAGTGGAGAAGCTGGTGCCCAGACCTGGC
AGTGGCAGCTCCTCAGGGTCCAGCAACTCAGGATCCCAGCCCGGGTCTCACCCTGGGTCTCAGAGTGGCTCCGGGGAACGCTTCAGAGTG
AGATCATCATCCAAGTCTGAAGGCTCTCCATCTCAGCGCCTGGAAAATGCAGTGAAAAAACCTGAAGATAAAAAGGAAGTTTTCAGACCC
CTCAAGCCTGCTGATCTGACCGCACTGGCCAAAGAGCTTCGAGCAGTGGAAGATGTACGGCCACCTCACAAAGTAACGGACTACTCCTCA
TCCAGTGAGGAGTCGGGGACGACGGATGAGGAGGACGACGATGTGGAGCAGGAAGGGGCTGACGAGTCCACCTCAGGACCAGAGGACACC
AGAGCAGCGTCATCTCTGAATTTGAGCAATGGTGAAACGGAATCTGTGAAAACCATGATTGTCCATGATGATGTAGAAAGTGAGCCGGCC
ATGACCCCATCCAAGGAGGGCACTCTAATCGTCCGCCAGAGTACAGTTGACCAAAAGCGTGCCAGCCATCATGAGAGCAATGGCTTTGCC
GGTCGCATTCACCTCTTGCCAGATCTCTTACAGCAAAGCCATTCCTCCTCCACTTCCTCCACCTCCTCCTCCCCATCCTCCAGCCAGCCG
ACACCCACCATGTCCCCACAGACACCCCAGGACAAGCTCACTGCTAATGAGACTCAGTCCGCTAGTAGCACACTCCAGAAACACAAATCT
TCCTCCTCCTTTACACCTTTTATAGACCCCAGATTACTACAGATTTCTCCATCTAGCGGAACAACAGTGACATCTGTGGTGGGATTTTCC
TGTGATGGGATGAGACCAGAAGCCATAAGGCAAGATCCTACCCGGAAAGGCTCAGTGGTCAATGTGAATCCTACCAACACTAGGCCACAG
AGTGACACCCCGGAGATTCGTAAATACAAGAAGAGGTTTAACTCTGAGATTCTGTGTGCTGCCTTATGGGGAGTGAATTTGCTAGTGGGT
ACAGAGAGTGGCCTGATGCTGCTGGACAGAAGTGGCCAAGGGAAGGTCTATCCTCTTATCAACCGAAGACGATTTCAACAAATGGACGTA
CTTGAGGGCTTGAATGTCTTGGTGACAATATCTGGCAAAAAGGATAAGTTACGTGTCTACTATTTGTCCTGGTTAAGAAATAAAATACTT
CACAATGATCCAGAAGTTGAGAAGAAGCAGGGATGGACAACCGTAGGGGATTTGGAAGGATGTGTACATTATAAAGTTGTAAAATATGAA
AGAATCAAATTTCTGGTGATTGCTTTGAAGAGTTCTGTGGAAGTCTATGCGTGGGCACCAAAGCCATATCACAAATTTATGGCCTTTAAG
TCATTTGGAGAATTGGTACATAAGCCATTACTGGTGGATCTCACTGTTGAGGAAGGCCAGAGGTTGAAAGTGATCTATGGATCCTGTGCT
GGATTCCATGCTGTTGATGTGGATTCAGGATCAGTCTATGACATTTATCTACCAACACATATCCAGTGTAGCATCAAACCCCATGCAATC
ATCATCCTCCCCAATACAGATGGAATGGAGCTTCTGGTGTGCTATGAAGATGAGGGGGTTTATGTAAACACATATGGAAGGATCACCAAG
GATGTAGTTCTACAGTGGGGAGAGATGCCTACATCAGTAGCATATATTCGATCCAATCAGACAATGGGCTGGGGAGAGAAGGCCATAGAG
ATCCGATCTGTGGAAACTGGTCACTTGGATGGTGTGTTCATGCACAAAAGGGCTCAAAGACTAAAATTCTTGTGTGAACGCAATGACAAG
GTGTTCTTTGCCTCTGTTCGGTCTGGTGGCAGCAGTCAGGTTTATTTCATGACCTTAGGCAGGACTTCTCTTCTGAGCTGGTAGAAGCAG
TGTGATCCAGGGATTACTGGCCTCCAGAGTCTTCAAGATCCTGAGAACTTGGAATTCCTTGTAACTGGAGCTCGGAGCTGCACCGAGGGC
AACCAGGACAGCTGTGTGTGCAGACCTCATGTGTTGGGTTCTCTCCCCTCCTTCCTGTTCCTCTTATATACCAGTTTATCCCCATTCTTT

>In-frame_ENST00000295317_ENST00000425019_TCGA-24-1553-01A_RNF149_chr2_101910476_-_MAP4K4_chr2_102407182_length(amino acids)=1502AA_start in transcript=75_stop in transcript=4583
MTPPSQLSGAAMAWRRREASVGARGVLALALLALALCVPGARGRALEWFSAVVNIEYVDPQTNLTVWSVSESGRFGDSSPKEGAHGLVGV
PWAPGGDLEGCAPDTRFFVPEPGGRGAAPWVALVARGGCTFKDKVLVAARRNASAVVLYNEERYGNITLPMSHAGTGNIVVIMISYPKGR
EILELVQKGIPVTMTIGVGTRHVQEFISGQSVVFVAIAFITMMIISLAWLIFYYIQRFLYTGSQIGSQSHRKETKKVIGQLLLHTVKHGE
KGRHVKTGQLAAIKVMDVTEDEEEEIKLEINMLKKYSHHRNIATYYGAFIKKSPPGHDDQLWLVMEFCGAGSITDLVKNTKGNTLKEDWI
AYISREILRGLAHLHIHHVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDLWSCG
ITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFFSFIEGCLVKNYMQRPSTEQLLKHPFIRDQPNERQVRIQLKDHIDRTR
KKRGEKDETEYEYSGSEEEEEEVPEQEGEPSSIVNVPGESTLRRDFLRLQQENKERSEALRRQQLLQEQQLREQEEYKRQLLAERQKRIE
QQKEQRRRLEEQQRREREARRQQEREQRRREQEEKRRLEELERRRKEEEERRRAEEEKRRVEREQEYIRRQLEEEQRHLEVLQQQLLQEQ
AMLLHDHRRPHPQHSQQPPPPQQERSKPSFHAPEPKAHYEPADRAREVEDRFRKTNHSSPEAQSKQTGRVLEPPVPSRSESFSNGNSESV
HPALQRPAEPQVPVRTTSRSPVLSRRDSPLQGSGQQNSQAGQRNSTSIEPRLLWERVEKLVPRPGSGSSSGSSNSGSQPGSHPGSQSGSG
ERFRVRSSSKSEGSPSQRLENAVKKPEDKKEVFRPLKPADLTALAKELRAVEDVRPPHKVTDYSSSSEESGTTDEEDDDVEQEGADESTS
GPEDTRAASSLNLSNGETESVKTMIVHDDVESEPAMTPSKEGTLIVRQSTVDQKRASHHESNGFAGRIHLLPDLLQQSHSSSTSSTSSSP
SSSQPTPTMSPQTPQDKLTANETQSASSTLQKHKSSSSFTPFIDPRLLQISPSSGTTVTSVVGFSCDGMRPEAIRQDPTRKGSVVNVNPT
NTRPQSDTPEIRKYKKRFNSEILCAALWGVNLLVGTESGLMLLDRSGQGKVYPLINRRRFQQMDVLEGLNVLVTISGKKDKLRVYYLSWL
RNKILHNDPEVEKKQGWTTVGDLEGCVHYKVVKYERIKFLVIALKSSVEVYAWAPKPYHKFMAFKSFGELVHKPLLVDLTVEEGQRLKVI
YGSCAGFHAVDVDSGSVYDIYLPTHIQCSIKPHAIIILPNTDGMELLVCYEDEGVYVNTYGRITKDVVLQWGEMPTSVAYIRSNQTMGWG

--------------------------------------------------------------
>In-frame_ENST00000295317_ENST00000324219_TCGA-24-1553-01A_RNF149_chr2_101910476_-_MAP4K4_chr2_102407182_length(transcript)=4807nt_BP=888nt
CGCCTCAGTCTTCCAGGGCGGCGGTGGGTGTCCGCTTCTCTCTGCTCTTCGACTGCACCGCACTCGCGCGTGACCCTGACTCCCCCTAGT
CAGCTCAGCGGTGCTGCCATGGCGTGGCGGCGGCGCGAAGCCAGCGTCGGGGCTCGCGGCGTGTTGGCTCTGGCGTTGCTCGCCCTGGCC
CTGTGCGTGCCCGGGGCCCGGGGCCGGGCTCTCGAGTGGTTCTCGGCCGTGGTAAACATCGAGTACGTGGACCCGCAGACCAACCTGACG
GTGTGGAGCGTCTCGGAGAGTGGCCGCTTCGGCGACAGCTCGCCCAAGGAGGGCGCGCATGGCCTGGTGGGCGTCCCGTGGGCGCCCGGC
GGAGACCTCGAGGGCTGCGCGCCCGACACGCGCTTCTTCGTGCCCGAGCCCGGCGGCCGAGGGGCCGCGCCCTGGGTCGCCCTGGTGGCT
CGTGGGGGCTGCACCTTCAAGGACAAGGTGCTGGTGGCGGCGCGGAGGAACGCCTCGGCCGTCGTCCTCTACAATGAGGAGCGCTACGGG
AACATCACCTTGCCCATGTCTCACGCGGGAACAGGAAATATAGTGGTCATTATGATTAGCTATCCAAAAGGAAGAGAAATTTTGGAGCTG
GTGCAAAAAGGAATTCCAGTAACGATGACCATAGGGGTTGGCACCCGGCATGTACAGGAGTTCATCAGCGGTCAGTCTGTGGTGTTTGTG
GCCATTGCCTTCATCACCATGATGATTATCTCGTTAGCCTGGCTAATATTTTACTATATACAGCGTTTCCTATATACTGGCTCTCAGATT
GGAAGTCAGAGCCATAGAAAAGAAACTAAGAAAGTTATTGGCCAGCTTCTACTTCATACTGTAAAGCATGGAGAAAAGGGTCGACATGTT
AAAACGGGTCAGTTGGCAGCCATCAAAGTTATGGATGTCACTGAGGATGAAGAGGAAGAAATCAAACTGGAGATAAATATGCTAAAGAAA
TACTCTCATCACAGAAACATTGCAACATATTATGGTGCTTTCATCAAAAAGAGCCCTCCAGGACATGATGACCAACTCTGGCTTGTTATG
GAGTTCTGTGGGGCTGGGTCCATTACAGACCTTGTGAAGAACACCAAAGGGAACACACTCAAAGAAGACTGGATCGCTTACATCTCCAGA
GAAATCCTGAGGGGACTGGCACATCTTCACATTCATCATGTGATTCACCGGGATATCAAGGGCCAGAATGTGTTGCTGACTGAGAATGCA
GAGGTGAAACTTGTTGACTTTGGTGTGAGTGCTCAGCTGGACAGGACTGTGGGGCGGAGAAATACGTTCATAGGCACTCCCTACTGGATG
GCTCCTGAGGTCATCGCCTGTGATGAGAACCCAGATGCCACCTATGATTACAGAAGTGATCTTTGGTCTTGTGGCATTACAGCCATTGAG
ATGGCAGAAGGTGCTCCCCCTCTCTGTGACATGCATCCAATGAGAGCACTGTTTCTCATTCCCAGAAACCCTCCTCCCCGGCTGAAGTCA
AAAAAATGGTCGAAGAAGTTTTTTAGTTTTATAGAAGGGTGCCTGGTGAAGAATTACATGCAGCGGCCCTCTACAGAGCAGCTTTTGAAA
CATCCTTTTATAAGGGATCAGCCAAATGAAAGGCAAGTTAGAATCCAGCTTAAGGATCATATAGATCGTACCAGGAAGAAGAGAGGCGAG
AAAGATGAAACTGAGTATGAGTACAGTGGGAGTGAGGAAGAAGAGGAGGAAGTGCCTGAACAGGAAGGAGAGCCAAGTTCCATTGTGAAC
GTGCCTGGTGAGTCTACTCTTCGCCGAGATTTCCTGAGACTGCAGCAGGAGAACAAGGAACGTTCCGAGGCTCTTCGGAGACAACAGTTA
CTACAGGAGCAACAGCTCCGGGAGCAGGAAGAATATAAAAGGCAACTGCTGGCAGAGAGACAGAAGCGGATTGAGCAGCAGAAAGAACAG
AGGCGACGGCTAGAAGAGCAACAAAGGAGAGAGCGGGAAGCTAGAAGGCAGCAGGAACGTGAACAGCGAAGGAGAGAACAAGAAGAAAAG
AGGCGTCTAGAGGAGTTGGAGAGAAGGCGCAAAGAAGAAGAGGAGAGGAGACGGGCAGAAGAAGAAAAGAGGAGAGTTGAAAGAGAACAG
GAGTATATCAGGCGACAGCTAGAAGAGGAGCAGCGGCACTTGGAAGTCCTTCAGCAGCAGCTGCTCCAGGAGCAGGCCATGTTACTGGAG
TGCCGATGGCGGGAGATGGAGGAGCACCGGCAGGCAGAGAGGCTCCAGAGGCAGTTGCAACAAGAACAAGCATATCTCCTGTCTCTACAG
CATGACCATAGGAGGCCGCACCCGCAGCACTCGCAGCAGCCGCCACCACCGCAGCAGGAAAGGAGCAAGCCAAGCTTCCATGCTCCCGAG
CCCAAAGCCCACTACGAGCCTGCTGACCGAGCGCGAGAGGTGGAAGATAGATTTAGGAAAACTAACCACAGCTCCCCTGAAGCCCAGTCT
AAGCAGACAGGCAGAGTATTGGAGCCACCAGTGCCTTCCCGATCAGAGTCTTTTTCCAATGGCAACTCCGAGTCTGTGCATCCCGCCCTG
CAGAGACCAGCGGAGCCACAGGTACAGTGGTCCCACCTGGCATCTCTCAAGAACAATGTTTCCCCTGTCTCGCGATCCCATTCCTTCAGT
GACCCTTCTCCCAAATTTGCACACCACCATCTTCGTTCTCAGGACCCATGTCCACCTTCCCGCAGTGAGGTGCTCAGTCAGAGCTCTGAC
TCTAAGTCAGAGGCGCCTGACCCTACCCAAAAGGCTTGGTCTAGATCAGACAGTGACGAGGTGCCTCCAAGGGTTCCTGTGAGAACAACA
TCTCGCTCCCCTGTTCTGTCCCGTCGAGATTCCCCACTGCAGGGCAGTGGGCAGCAGAATAGCCAGGCAGGACAGAGAAACTCCACCAGC
AGTATTGAGCCCAGGCTTCTGTGGGAGAGAGTGGAGAAGCTGGTGCCCAGACCTGGCAGTGGCAGCTCCTCAGGGTCCAGCAACTCAGGA
TCCCAGCCCGGGTCTCACCCTGGGTCTCAGAGTGGCTCCGGGGAACGCTTCAGAGTGAGATCATCATCCAAGTCTGAAGGCTCTCCATCT
CAGCGCCTGGAAAATGCAGTGAAAAAACCTGAAGATAAAAAGGAAGTTTTCAGACCCCTCAAGCCTGCTGTAAGGATTGATCTGACCGCA
CTGGCCAAAGAGCTTCGAGCAGTGGAAGATGTACGGCCACCTCACAAAGTAACGGACTACTCCTCATCCAGTGAGGAGTCGGGGACGACG
GATGAGGAGGACGACGATGTGGAGCAGGAAGGGGCTGACGAGTCCACCTCAGGACCAGAGGACACCAGAGCAGCGTCATCTCTGAATTTG
AGCAATGGTGAAACGGAATCTGTGAAAACCATGATTGTCCATGATGATGTAGAAAGTGAGCCGGCCATGACCCCATCCAAGGAGGGCACT
CTAATCGTCCGCCAGACTCAGTCCGCTAGTAGCACACTCCAGAAACACAAATCTTCCTCCTCCTTTACACCTTTTATAGACCCCAGATTA
CTACAGATTTCTCCATCTAGCGGAACAACAGTGACATCTGTGGTGGGATTTTCCTGTGATGGGATGAGACCAGAAGCCATAAGGCAAGAT
CCTACCCGGAAAGGCTCAGTGGTCAATGTGAATCCTACCAACACTAGGCCACAGAGTGACACCCCGGAGATTCGTAAATACAAGAAGAGG
TTTAACTCTGAGATTCTGTGTGCTGCCTTATGGGGAGTGAATTTGCTAGTGGGTACAGAGAGTGGCCTGATGCTGCTGGACAGAAGTGGC
CAAGGGAAGGTCTATCCTCTTATCAACCGAAGACGATTTCAACAAATGGACGTACTTGAGGGCTTGAATGTCTTGGTGACAATATCTGGC
AAAAAGGATAAGTTACGTGTCTACTATTTGTCCTGGTTAAGAAATAAAATACTTCACAATGATCCAGAAGTTGAGAAGAAGCAGGGATGG
ACAACCGTAGGGGATTTGGAAGGATGTGTACATTATAAAGTTGTAAAATATGAAAGAATCAAATTTCTGGTGATTGCTTTGAAGAGTTCT
GTGGAAGTCTATGCGTGGGCACCAAAGCCATATCACAAATTTATGGCCTTTAAGTCATTTGGAGAATTGGTACATAAGCCATTACTGGTG
GATCTCACTGTTGAGGAAGGCCAGAGGTTGAAAGTGATCTATGGATCCTGTGCTGGATTCCATGCTGTTGATGTGGATTCAGGATCAGTC
TATGACATTTATCTACCAACACATATCCAGTGTAGCATCAAACCCCATGCAATCATCATCCTCCCCAATACAGATGGAATGGAGCTTCTG
GTGTGCTATGAAGATGAGGGGGTTTATGTAAACACATATGGAAGGATCACCAAGGATGTAGTTCTACAGTGGGGAGAGATGCCTACATCA
GTAGCATATATTCGATCCAATCAGACAATGGGCTGGGGAGAGAAGGCCATAGAGATCCGATCTGTGGAAACTGGTCACTTGGATGGTGTG
TTCATGCACAAAAGGGCTCAAAGACTAAAATTCTTGTGTGAACGCAATGACAAGGTGTTCTTTGCCTCTGTTCGGTCTGGTGGCAGCAGT
CAGGTTTATTTCATGACCTTAGGCAGGACTTCTCTTCTGAGCTGGTAGAAGCAGTGTGATCCAGGGATTACTGGCCTCCAGAGTCTTCAA

>In-frame_ENST00000295317_ENST00000324219_TCGA-24-1553-01A_RNF149_chr2_101910476_-_MAP4K4_chr2_102407182_length(amino acids)=1550AA_start in transcript=75_stop in transcript=4727
MTPPSQLSGAAMAWRRREASVGARGVLALALLALALCVPGARGRALEWFSAVVNIEYVDPQTNLTVWSVSESGRFGDSSPKEGAHGLVGV
PWAPGGDLEGCAPDTRFFVPEPGGRGAAPWVALVARGGCTFKDKVLVAARRNASAVVLYNEERYGNITLPMSHAGTGNIVVIMISYPKGR
EILELVQKGIPVTMTIGVGTRHVQEFISGQSVVFVAIAFITMMIISLAWLIFYYIQRFLYTGSQIGSQSHRKETKKVIGQLLLHTVKHGE
KGRHVKTGQLAAIKVMDVTEDEEEEIKLEINMLKKYSHHRNIATYYGAFIKKSPPGHDDQLWLVMEFCGAGSITDLVKNTKGNTLKEDWI
AYISREILRGLAHLHIHHVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDLWSCG
ITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFFSFIEGCLVKNYMQRPSTEQLLKHPFIRDQPNERQVRIQLKDHIDRTR
KKRGEKDETEYEYSGSEEEEEEVPEQEGEPSSIVNVPGESTLRRDFLRLQQENKERSEALRRQQLLQEQQLREQEEYKRQLLAERQKRIE
QQKEQRRRLEEQQRREREARRQQEREQRRREQEEKRRLEELERRRKEEEERRRAEEEKRRVEREQEYIRRQLEEEQRHLEVLQQQLLQEQ
AMLLECRWREMEEHRQAERLQRQLQQEQAYLLSLQHDHRRPHPQHSQQPPPPQQERSKPSFHAPEPKAHYEPADRAREVEDRFRKTNHSS
PEAQSKQTGRVLEPPVPSRSESFSNGNSESVHPALQRPAEPQVQWSHLASLKNNVSPVSRSHSFSDPSPKFAHHHLRSQDPCPPSRSEVL
SQSSDSKSEAPDPTQKAWSRSDSDEVPPRVPVRTTSRSPVLSRRDSPLQGSGQQNSQAGQRNSTSSIEPRLLWERVEKLVPRPGSGSSSG
SSNSGSQPGSHPGSQSGSGERFRVRSSSKSEGSPSQRLENAVKKPEDKKEVFRPLKPAVRIDLTALAKELRAVEDVRPPHKVTDYSSSSE
ESGTTDEEDDDVEQEGADESTSGPEDTRAASSLNLSNGETESVKTMIVHDDVESEPAMTPSKEGTLIVRQTQSASSTLQKHKSSSSFTPF
IDPRLLQISPSSGTTVTSVVGFSCDGMRPEAIRQDPTRKGSVVNVNPTNTRPQSDTPEIRKYKKRFNSEILCAALWGVNLLVGTESGLML
LDRSGQGKVYPLINRRRFQQMDVLEGLNVLVTISGKKDKLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDLEGCVHYKVVKYERIKFLVI
ALKSSVEVYAWAPKPYHKFMAFKSFGELVHKPLLVDLTVEEGQRLKVIYGSCAGFHAVDVDSGSVYDIYLPTHIQCSIKPHAIIILPNTD
GMELLVCYEDEGVYVNTYGRITKDVVLQWGEMPTSVAYIRSNQTMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVR

--------------------------------------------------------------
>In-frame_ENST00000295317_ENST00000350198_TCGA-24-1553-01A_RNF149_chr2_101910476_-_MAP4K4_chr2_102407182_length(transcript)=4345nt_BP=888nt
CGCCTCAGTCTTCCAGGGCGGCGGTGGGTGTCCGCTTCTCTCTGCTCTTCGACTGCACCGCACTCGCGCGTGACCCTGACTCCCCCTAGT
CAGCTCAGCGGTGCTGCCATGGCGTGGCGGCGGCGCGAAGCCAGCGTCGGGGCTCGCGGCGTGTTGGCTCTGGCGTTGCTCGCCCTGGCC
CTGTGCGTGCCCGGGGCCCGGGGCCGGGCTCTCGAGTGGTTCTCGGCCGTGGTAAACATCGAGTACGTGGACCCGCAGACCAACCTGACG
GTGTGGAGCGTCTCGGAGAGTGGCCGCTTCGGCGACAGCTCGCCCAAGGAGGGCGCGCATGGCCTGGTGGGCGTCCCGTGGGCGCCCGGC
GGAGACCTCGAGGGCTGCGCGCCCGACACGCGCTTCTTCGTGCCCGAGCCCGGCGGCCGAGGGGCCGCGCCCTGGGTCGCCCTGGTGGCT
CGTGGGGGCTGCACCTTCAAGGACAAGGTGCTGGTGGCGGCGCGGAGGAACGCCTCGGCCGTCGTCCTCTACAATGAGGAGCGCTACGGG
AACATCACCTTGCCCATGTCTCACGCGGGAACAGGAAATATAGTGGTCATTATGATTAGCTATCCAAAAGGAAGAGAAATTTTGGAGCTG
GTGCAAAAAGGAATTCCAGTAACGATGACCATAGGGGTTGGCACCCGGCATGTACAGGAGTTCATCAGCGGTCAGTCTGTGGTGTTTGTG
GCCATTGCCTTCATCACCATGATGATTATCTCGTTAGCCTGGCTAATATTTTACTATATACAGCGTTTCCTATATACTGGCTCTCAGATT
GGAAGTCAGAGCCATAGAAAAGAAACTAAGAAAGTTATTGGCCAGCTTCTACTTCATACTGTAAAGCATGGAGAAAAGGGTCGACATGTT
AAAACGGGTCAGTTGGCAGCCATCAAAGTTATGGATGTCACTGAGGATGAAGAGGAAGAAATCAAACTGGAGATAAATATGCTAAAGAAA
TACTCTCATCACAGAAACATTGCAACATATTATGGTGCTTTCATCAAAAAGAGCCCTCCAGGACATGATGACCAACTCTGGCTTGTTATG
GAGTTCTGTGGGGCTGGGTCCATTACAGACCTTGTGAAGAACACCAAAGGGAACACACTCAAAGAAGACTGGATCGCTTACATCTCCAGA
GAAATCCTGAGGGGACTGGCACATCTTCACATTCATCATGTGATTCACCGGGATATCAAGGGCCAGAATGTGTTGCTGACTGAGAATGCA
GAGGTGAAACTTGTTGACTTTGGTGTGAGTGCTCAGCTGGACAGGACTGTGGGGCGGAGAAATACGTTCATAGGCACTCCCTACTGGATG
GCTCCTGAGGTCATCGCCTGTGATGAGAACCCAGATGCCACCTATGATTACAGAAGTGATCTTTGGTCTTGTGGCATTACAGCCATTGAG
ATGGCAGAAGGTGCTCCCCCTCTCTGTGACATGCATCCAATGAGAGCACTGTTTCTCATTCCCAGAAACCCTCCTCCCCGGCTGAAGTCA
AAAAAATGGTCGAAGAAGTTTTTTAGTTTTATAGAAGGGTGCCTGGTGAAGAATTACATGCAGCGGCCCTCTACAGAGCAGCTTTTGAAA
CATCCTTTTATAAGGGATCAGCCAAATGAAAGGCAAGTTAGAATCCAGCTTAAGGATCATATAGATCGTACCAGGAAGAAGAGAGGCGAG
AAAGATGAAACTGAGTATGAGTACAGTGGGAGTGAGGAAGAAGAGGAGGAAGTGCCTGAACAGGAAGGAGAGCCAAGTTCCATTGTGAAC
GTGCCTGGTGAGTCTACTCTTCGCCGAGATTTCCTGAGACTGCAGCAGGAGAACAAGGAACGTTCCGAGGCTCTTCGGAGACAACAGTTA
CTACAGGAGCAACAGCTCCGGGAGCAGGAAGAATATAAAAGGCAACTGCTGGCAGAGAGACAGAAGCGGATTGAGCAGCAGAAAGAACAG
AGGCGACGGCTAGAAGAGCAACAAAGGAGAGAGCGGGAAGCTAGAAGGCAGCAGGAACGTGAACAGCGAAGGAGAGAACAAGAAGAAAAG
AGGCGTCTAGAGGAGTTGGAGAGAAGGCGCAAAGAAGAAGAGGAGAGGAGACGGGCAGAAGAAGAAAAGAGGAGAGTTGAAAGAGAACAG
GAGTATATCAGGCGACAGCTAGAAGAGGAGCAGCGGCACTTGGAAGTCCTTCAGCAGCAGCTGCTCCAGGAGCAGGCCATGTTACTGCAT
GACCATAGGAGGCCGCACCCGCAGCACTCGCAGCAGCCGCCACCACCGCAGCAGGAAAGGAGCAAGCCAAGCTTCCATGCTCCCGAGCCC
AAAGCCCACTACGAGCCTGCTGACCGAGCGCGAGAGGTTCCTGTGAGAACAACATCTCGCTCCCCTGTTCTGTCCCGTCGAGATTCCCCA
CTGCAGGGCAGTGGGCAGCAGAATAGCCAGGCAGGACAGAGAAACTCCACCAGCAGTATTGAGCCCAGGCTTCTGTGGGAGAGAGTGGAG
AAGCTGGTGCCCAGACCTGGCAGTGGCAGCTCCTCAGGGTCCAGCAACTCAGGATCCCAGCCCGGGTCTCACCCTGGGTCTCAGAGTGGC
TCCGGGGAACGCTTCAGAGTGAGATCATCATCCAAGTCTGAAGGCTCTCCATCTCAGCGCCTGGAAAATGCAGTGAAAAAACCTGAAGAT
AAAAAGGAAGTTTTCAGACCCCTCAAGCCTGCTGTAAGGATTGATCTGACCGCACTGGCCAAAGAGCTTCGAGCAGTGGAAGATGTACGG
CCACCTCACAAAGTAACGGACTACTCCTCATCCAGTGAGGAGTCGGGGACGACGGATGAGGAGGACGACGATGTGGAGCAGGAAGGGGCT
GACGAGTCCACCTCAGGACCAGAGGACACCAGAGCAGCGTCATCTCTGAATTTGAGCAATGGTGAAACGGAATCTGTGAAAACCATGATT
GTCCATGATGATGTAGAAAGTGAGCCGGCCATGACCCCATCCAAGGAGGGCACTCTAATCGTCCGCCAGACTCAGTCCGCTAGTAGCACA
CTCCAGAAACACAAATCTTCCTCCTCCTTTACACCTTTTATAGACCCCAGATTACTACAGATTTCTCCATCTAGCGGAACAACAGTGACA
TCTGTGGTGGGATTTTCCTGTGATGGGATGAGACCAGAAGCCATAAGGCAAGATCCTACCCGGAAAGGCTCAGTGGTCAATGTGAATCCT
ACCAACACTAGGCCACAGAGTGACACCCCGGAGATTCGTAAATACAAGAAGAGGTTTAACTCTGAGATTCTGTGTGCTGCCTTATGGGGA
GTGAATTTGCTAGTGGGTACAGAGAGTGGCCTGATGCTGCTGGACAGAAGTGGCCAAGGGAAGGTCTATCCTCTTATCAACCGAAGACGA
TTTCAACAAATGGACGTACTTGAGGGCTTGAATGTCTTGGTGACAATATCTGGCAAAAAGGATAAGTTACGTGTCTACTATTTGTCCTGG
TTAAGAAATAAAATACTTCACAATGATCCAGAAGTTGAGAAGAAGCAGGGATGGACAACCGTAGGGGATTTGGAAGGATGTGTACATTAT
AAAGTTGTAAAATATGAAAGAATCAAATTTCTGGTGATTGCTTTGAAGAGTTCTGTGGAAGTCTATGCGTGGGCACCAAAGCCATATCAC
AAATTTATGGCCTTTAAGTCATTTGGAGAATTGGTACATAAGCCATTACTGGTGGATCTCACTGTTGAGGAAGGCCAGAGGTTGAAAGTG
ATCTATGGATCCTGTGCTGGATTCCATGCTGTTGATGTGGATTCAGGATCAGTCTATGACATTTATCTACCAACACATGTAAGAAAGAAC
CCACACTCTATGATCCAGTGTAGCATCAAACCCCATGCAATCATCATCCTCCCCAATACAGATGGAATGGAGCTTCTGGTGTGCTATGAA
GATGAGGGGGTTTATGTAAACACATATGGAAGGATCACCAAGGATGTAGTTCTACAGTGGGGAGAGATGCCTACATCAGTAGCATATATT
CGATCCAATCAGACAATGGGCTGGGGAGAGAAGGCCATAGAGATCCGATCTGTGGAAACTGGTCACTTGGATGGTGTGTTCATGCACAAA
AGGGCTCAAAGACTAAAATTCTTGTGTGAACGCAATGACAAGGTGTTCTTTGCCTCTGTTCGGTCTGGTGGCAGCAGTCAGGTTTATTTC
ATGACCTTAGGCAGGACTTCTCTTCTGAGCTGGTAGAAGCAGTGTGATCCAGGGATTACTGGCCTCCAGAGTCTTCAAGATCCTGAGAAC

>In-frame_ENST00000295317_ENST00000350198_TCGA-24-1553-01A_RNF149_chr2_101910476_-_MAP4K4_chr2_102407182_length(amino acids)=1396AA_start in transcript=75_stop in transcript=4265
MTPPSQLSGAAMAWRRREASVGARGVLALALLALALCVPGARGRALEWFSAVVNIEYVDPQTNLTVWSVSESGRFGDSSPKEGAHGLVGV
PWAPGGDLEGCAPDTRFFVPEPGGRGAAPWVALVARGGCTFKDKVLVAARRNASAVVLYNEERYGNITLPMSHAGTGNIVVIMISYPKGR
EILELVQKGIPVTMTIGVGTRHVQEFISGQSVVFVAIAFITMMIISLAWLIFYYIQRFLYTGSQIGSQSHRKETKKVIGQLLLHTVKHGE
KGRHVKTGQLAAIKVMDVTEDEEEEIKLEINMLKKYSHHRNIATYYGAFIKKSPPGHDDQLWLVMEFCGAGSITDLVKNTKGNTLKEDWI
AYISREILRGLAHLHIHHVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDLWSCG
ITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFFSFIEGCLVKNYMQRPSTEQLLKHPFIRDQPNERQVRIQLKDHIDRTR
KKRGEKDETEYEYSGSEEEEEEVPEQEGEPSSIVNVPGESTLRRDFLRLQQENKERSEALRRQQLLQEQQLREQEEYKRQLLAERQKRIE
QQKEQRRRLEEQQRREREARRQQEREQRRREQEEKRRLEELERRRKEEEERRRAEEEKRRVEREQEYIRRQLEEEQRHLEVLQQQLLQEQ
AMLLHDHRRPHPQHSQQPPPPQQERSKPSFHAPEPKAHYEPADRAREVPVRTTSRSPVLSRRDSPLQGSGQQNSQAGQRNSTSSIEPRLL
WERVEKLVPRPGSGSSSGSSNSGSQPGSHPGSQSGSGERFRVRSSSKSEGSPSQRLENAVKKPEDKKEVFRPLKPAVRIDLTALAKELRA
VEDVRPPHKVTDYSSSSEESGTTDEEDDDVEQEGADESTSGPEDTRAASSLNLSNGETESVKTMIVHDDVESEPAMTPSKEGTLIVRQTQ
SASSTLQKHKSSSSFTPFIDPRLLQISPSSGTTVTSVVGFSCDGMRPEAIRQDPTRKGSVVNVNPTNTRPQSDTPEIRKYKKRFNSEILC
AALWGVNLLVGTESGLMLLDRSGQGKVYPLINRRRFQQMDVLEGLNVLVTISGKKDKLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDLE
GCVHYKVVKYERIKFLVIALKSSVEVYAWAPKPYHKFMAFKSFGELVHKPLLVDLTVEEGQRLKVIYGSCAGFHAVDVDSGSVYDIYLPT
HVRKNPHSMIQCSIKPHAIIILPNTDGMELLVCYEDEGVYVNTYGRITKDVVLQWGEMPTSVAYIRSNQTMGWGEKAIEIRSVETGHLDG

--------------------------------------------------------------
>In-frame_ENST00000295317_ENST00000302217_TCGA-24-1553-01A_RNF149_chr2_101910476_-_MAP4K4_chr2_102407182_length(transcript)=3973nt_BP=888nt
CGCCTCAGTCTTCCAGGGCGGCGGTGGGTGTCCGCTTCTCTCTGCTCTTCGACTGCACCGCACTCGCGCGTGACCCTGACTCCCCCTAGT
CAGCTCAGCGGTGCTGCCATGGCGTGGCGGCGGCGCGAAGCCAGCGTCGGGGCTCGCGGCGTGTTGGCTCTGGCGTTGCTCGCCCTGGCC
CTGTGCGTGCCCGGGGCCCGGGGCCGGGCTCTCGAGTGGTTCTCGGCCGTGGTAAACATCGAGTACGTGGACCCGCAGACCAACCTGACG
GTGTGGAGCGTCTCGGAGAGTGGCCGCTTCGGCGACAGCTCGCCCAAGGAGGGCGCGCATGGCCTGGTGGGCGTCCCGTGGGCGCCCGGC
GGAGACCTCGAGGGCTGCGCGCCCGACACGCGCTTCTTCGTGCCCGAGCCCGGCGGCCGAGGGGCCGCGCCCTGGGTCGCCCTGGTGGCT
CGTGGGGGCTGCACCTTCAAGGACAAGGTGCTGGTGGCGGCGCGGAGGAACGCCTCGGCCGTCGTCCTCTACAATGAGGAGCGCTACGGG
AACATCACCTTGCCCATGTCTCACGCGGGAACAGGAAATATAGTGGTCATTATGATTAGCTATCCAAAAGGAAGAGAAATTTTGGAGCTG
GTGCAAAAAGGAATTCCAGTAACGATGACCATAGGGGTTGGCACCCGGCATGTACAGGAGTTCATCAGCGGTCAGTCTGTGGTGTTTGTG
GCCATTGCCTTCATCACCATGATGATTATCTCGTTAGCCTGGCTAATATTTTACTATATACAGCGTTTCCTATATACTGGCTCTCAGATT
GGAAGTCAGAGCCATAGAAAAGAAACTAAGAAAGTTATTGGCCAGCTTCTACTTCATACTGTAAAGCATGGAGAAAAGGGTCGACATGTT
AAAACGGGTCAGTTGGCAGCCATCAAAGTTATGGATGTCACTGAGGATGAAGAGGAAGAAATCAAACTGGAGATAAATATGCTAAAGAAA
TACTCTCATCACAGAAACATTGCAACATATTATGGTGCTTTCATCAAAAAGAGCCCTCCAGGACATGATGACCAACTCTGGCTTGTTATG
GAGTTCTGTGGGGCTGGGTCCATTACAGACCTTGTGAAGAACACCAAAGGGAACACACTCAAAGAAGACTGGATCGCTTACATCTCCAGA
GAAATCCTGAGGGGACTGGCACATCTTCACATTCATCATGTGATTCACCGGGATATCAAGGGCCAGAATGTGTTGCTGACTGAGAATGCA
GAGGTGAAACTTGATGAAACTGAGTATGAGTACAGTGGGAGTGAGGAAGAAGAGGAGGAAGTGCCTGAACAGGAAGGAGAGCCAAGTTCC
ATTGTGAACGTGCCTGGTGAGTCTACTCTTCGCCGAGATTTCCTGAGACTGCAGCAGGAGAACAAGGAACGTTCCGAGGCTCTTCGGAGA
CAACAGTTACTACAGGAGCAACAGCTCCGGGAGCAGGAAGAATATAAAAGGCAACTGCTGGCAGAGAGACAGAAGCGGATTGAGCAGCAG
AAAGAACAGAGGCGACGGCTAGAAGAGCAACAAAGGAGAGAGCGGGAAGCTAGAAGGCAGCAGGAACGTGAACAGCGAAGGAGAGAACAA
GAAGAAAAGAGGCGTCTAGAGGAGTTGGAGAGAAGGCGCAAAGAAGAAGAGGAGAGGAGACGGGCAGAAGAAGAAAAGAGGAGAGTTGAA
AGAGAACAGGAGTATATCAGGCGACAGCTAGAAGAGGAGCAGCGGCACTTGGAAGTCCTTCAGCAGCAGCTGCTCCAGGAGCAGGCCATG
TTACTGGAGTGCCGATGGCGGGAGATGGAGGAGCACCGGCAGGCAGAGAGGCTCCAGAGGCAGTTGCAACAAGAACAAGCATATCTCCTG
TCTCTACAGCATGACCATAGGAGGCCGCACCCGCAGCACTCGCAGCAGCCGCCACCACCGCAGCAGGAAAGGAGCAAGCCAAGCTTCCAT
GCTCCCGAGCCCAAAGCCCACTACGAGCCTGCTGACCGAGCGCGAGAGGTTCCTGTGAGAACAACATCTCGCTCCCCTGTTCTGTCCCGT
CGAGATTCCCCACTGCAGGGCAGTGGGCAGCAGAATAGCCAGGCAGGACAGAGAAACTCCACCAGCAGTATTGAGCCCAGGCTTCTGTGG
GAGAGAGTGGAGAAGCTGGTGCCCAGACCTGGCAGTGGCAGCTCCTCAGGGTCCAGCAACTCAGGATCCCAGCCCGGGTCTCACCCTGGG
TCTCAGAGTGGCTCCGGGGAACGCTTCAGAGTGAGATCATCATCCAAGTCTGAAGGCTCTCCATCTCAGCGCCTGGAAAATGCAGTGAAA
AAACCTGAAGATAAAAAGGAAGTTTTCAGACCCCTCAAGCCTGCTGTAAGGATTGATCTGACCGCACTGGCCAAAGAGCTTCGAGCAGTG
GAAGATGTACGGCCACCTCACAAAGTAACGGACTACTCCTCATCCAGTGAGGAGTCGGGGACGACGGATGAGGAGGACGACGATGTGGAG
CAGGAAGGGGCTGACGAGTCCACCTCAGGACCAGAGGACACCAGAGCAGCGTCATCTCTGAATTTGAGCAATGGTGAAACGGAATCTGTG
AAAACCATGATTGTCCATGATGATGTAGAAAGTGAGCCGGCCATGACCCCATCCAAGGAGGGCACTCTAATCGTCCGCCAGACTCAGTCC
GCTAGTAGCACACTCCAGAAACACAAATCTTCCTCCTCCTTTACACCTTTTATAGACCCCAGATTACTACAGATTTCTCCATCTAGCGGA
ACAACAGTGACATCTGTGGTGGGATTTTCCTGTGATGGGATGAGACCAGAAGCCATAAGGCAAGATCCTACCCGGAAAGGCTCAGTGGTC
AATGTGAATCCTACCAACACTAGGCCACAGAGTGACACCCCGGAGATTCGTAAATACAAGAAGAGGTTTAACTCTGAGATTCTGTGTGCT
GCCTTATGGGGAGTGAATTTGCTAGTGGGTACAGAGAGTGGCCTGATGCTGCTGGACAGAAGTGGCCAAGGGAAGGTCTATCCTCTTATC
AACCGAAGACGATTTCAACAAATGGACGTACTTGAGGGCTTGAATGTCTTGGTGACAATATCTGGCAAAAAGGATAAGTTACGTGTCTAC
TATTTGTCCTGGTTAAGAAATAAAATACTTCACAATGATCCAGAAGTTGAGAAGAAGCAGGGATGGACAACCGTAGGGGATTTGGAAGGA
TGTGTACATTATAAAGTTGTAAAATATGAAAGAATCAAATTTCTGGTGATTGCTTTGAAGAGTTCTGTGGAAGTCTATGCGTGGGCACCA
AAGCCATATCACAAATTTATGGCCTTTAAGTCATTTGGAGAATTGGTACATAAGCCATTACTGGTGGATCTCACTGTTGAGGAAGGCCAG
AGGTTGAAAGTGATCTATGGATCCTGTGCTGGATTCCATGCTGTTGATGTGGATTCAGGATCAGTCTATGACATTTATCTACCAACACAT
ATCCAGTGTAGCATCAAACCCCATGCAATCATCATCCTCCCCAATACAGATGGAATGGAGCTTCTGGTGTGCTATGAAGATGAGGGGGTT
TATGTAAACACATATGGAAGGATCACCAAGGATGTAGTTCTACAGTGGGGAGAGATGCCTACATCAGTAGCATATATTCGATCCAATCAG
ACAATGGGCTGGGGAGAGAAGGCCATAGAGATCCGATCTGTGGAAACTGGTCACTTGGATGGTGTGTTCATGCACAAAAGGGCTCAAAGA
CTAAAATTCTTGTGTGAACGCAATGACAAGGTGTTCTTTGCCTCTGTTCGGTCTGGTGGCAGCAGTCAGGTTTATTTCATGACCTTAGGC
AGGACTTCTCTTCTGAGCTGGTAGAAGCAGTGTGATCCAGGGATTACTGGCCTCCAGAGTCTTCAAGATCCTGAGAACTTGGAATTCCTT

>In-frame_ENST00000295317_ENST00000302217_TCGA-24-1553-01A_RNF149_chr2_101910476_-_MAP4K4_chr2_102407182_length(amino acids)=1272AA_start in transcript=75_stop in transcript=3893
MTPPSQLSGAAMAWRRREASVGARGVLALALLALALCVPGARGRALEWFSAVVNIEYVDPQTNLTVWSVSESGRFGDSSPKEGAHGLVGV
PWAPGGDLEGCAPDTRFFVPEPGGRGAAPWVALVARGGCTFKDKVLVAARRNASAVVLYNEERYGNITLPMSHAGTGNIVVIMISYPKGR
EILELVQKGIPVTMTIGVGTRHVQEFISGQSVVFVAIAFITMMIISLAWLIFYYIQRFLYTGSQIGSQSHRKETKKVIGQLLLHTVKHGE
KGRHVKTGQLAAIKVMDVTEDEEEEIKLEINMLKKYSHHRNIATYYGAFIKKSPPGHDDQLWLVMEFCGAGSITDLVKNTKGNTLKEDWI
AYISREILRGLAHLHIHHVIHRDIKGQNVLLTENAEVKLDETEYEYSGSEEEEEEVPEQEGEPSSIVNVPGESTLRRDFLRLQQENKERS
EALRRQQLLQEQQLREQEEYKRQLLAERQKRIEQQKEQRRRLEEQQRREREARRQQEREQRRREQEEKRRLEELERRRKEEEERRRAEEE
KRRVEREQEYIRRQLEEEQRHLEVLQQQLLQEQAMLLECRWREMEEHRQAERLQRQLQQEQAYLLSLQHDHRRPHPQHSQQPPPPQQERS
KPSFHAPEPKAHYEPADRAREVPVRTTSRSPVLSRRDSPLQGSGQQNSQAGQRNSTSSIEPRLLWERVEKLVPRPGSGSSSGSSNSGSQP
GSHPGSQSGSGERFRVRSSSKSEGSPSQRLENAVKKPEDKKEVFRPLKPAVRIDLTALAKELRAVEDVRPPHKVTDYSSSSEESGTTDEE
DDDVEQEGADESTSGPEDTRAASSLNLSNGETESVKTMIVHDDVESEPAMTPSKEGTLIVRQTQSASSTLQKHKSSSSFTPFIDPRLLQI
SPSSGTTVTSVVGFSCDGMRPEAIRQDPTRKGSVVNVNPTNTRPQSDTPEIRKYKKRFNSEILCAALWGVNLLVGTESGLMLLDRSGQGK
VYPLINRRRFQQMDVLEGLNVLVTISGKKDKLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDLEGCVHYKVVKYERIKFLVIALKSSVEV
YAWAPKPYHKFMAFKSFGELVHKPLLVDLTVEEGQRLKVIYGSCAGFHAVDVDSGSVYDIYLPTHIQCSIKPHAIIILPNTDGMELLVCY
EDEGVYVNTYGRITKDVVLQWGEMPTSVAYIRSNQTMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVY

--------------------------------------------------------------
>In-frame_ENST00000295317_ENST00000413150_TCGA-24-1553-01A_RNF149_chr2_101910476_-_MAP4K4_chr2_102407182_length(transcript)=7668nt_BP=888nt
CGCCTCAGTCTTCCAGGGCGGCGGTGGGTGTCCGCTTCTCTCTGCTCTTCGACTGCACCGCACTCGCGCGTGACCCTGACTCCCCCTAGT
CAGCTCAGCGGTGCTGCCATGGCGTGGCGGCGGCGCGAAGCCAGCGTCGGGGCTCGCGGCGTGTTGGCTCTGGCGTTGCTCGCCCTGGCC
CTGTGCGTGCCCGGGGCCCGGGGCCGGGCTCTCGAGTGGTTCTCGGCCGTGGTAAACATCGAGTACGTGGACCCGCAGACCAACCTGACG
GTGTGGAGCGTCTCGGAGAGTGGCCGCTTCGGCGACAGCTCGCCCAAGGAGGGCGCGCATGGCCTGGTGGGCGTCCCGTGGGCGCCCGGC
GGAGACCTCGAGGGCTGCGCGCCCGACACGCGCTTCTTCGTGCCCGAGCCCGGCGGCCGAGGGGCCGCGCCCTGGGTCGCCCTGGTGGCT
CGTGGGGGCTGCACCTTCAAGGACAAGGTGCTGGTGGCGGCGCGGAGGAACGCCTCGGCCGTCGTCCTCTACAATGAGGAGCGCTACGGG
AACATCACCTTGCCCATGTCTCACGCGGGAACAGGAAATATAGTGGTCATTATGATTAGCTATCCAAAAGGAAGAGAAATTTTGGAGCTG
GTGCAAAAAGGAATTCCAGTAACGATGACCATAGGGGTTGGCACCCGGCATGTACAGGAGTTCATCAGCGGTCAGTCTGTGGTGTTTGTG
GCCATTGCCTTCATCACCATGATGATTATCTCGTTAGCCTGGCTAATATTTTACTATATACAGCGTTTCCTATATACTGGCTCTCAGATT
GGAAGTCAGAGCCATAGAAAAGAAACTAAGAAAGTTATTGGCCAGCTTCTACTTCATACTGTAAAGCATGGAGAAAAGGGTCGACATGTT
AAAACGGGTCAGTTGGCAGCCATCAAAGTTATGGATGTCACTGAGGATGAAGAGGAAGAAATCAAACTGGAGATAAATATGCTAAAGAAA
TACTCTCATCACAGAAACATTGCAACATATTATGGTGCTTTCATCAAAAAGAGCCCTCCAGGACATGATGACCAACTCTGGCTTGTTATG
GAGTTCTGTGGGGCTGGGTCCATTACAGACCTTGTGAAGAACACCAAAGGGAACACACTCAAAGAAGACTGGATCGCTTACATCTCCAGA
GAAATCCTGAGGGGACTGGCACATCTTCACATTCATCATGTGATTCACCGGGATATCAAGGGCCAGAATGTGTTGCTGACTGAGAATGCA
GAGGTGAAACTTGTTGACTTTGGTGTGAGTGCTCAGCTGGACAGGACTGTGGGGCGGAGAAATACGTTCATAGGCACTCCCTACTGGATG
GCTCCTGAGGTCATCGCCTGTGATGAGAACCCAGATGCCACCTATGATTACAGAAGTGATCTTTGGTCTTGTGGCATTACAGCCATTGAG
ATGGCAGAAGGTGCTCCCCCTCTCTGTGACATGCATCCAATGAGAGCACTGTTTCTCATTCCCAGAAACCCTCCTCCCCGGCTGAAGTCA
AAAAAATGGTCGAAGAAGTTTTTTAGTTTTATAGAAGGGTGCCTGGTGAAGAATTACATGCAGCGGCCCTCTACAGAGCAGCTTTTGAAA
CATCCTTTTATAAGGGATCAGCCAAATGAAAGGCAAGTTAGAATCCAGCTTAAGGATCATATAGATCGTACCAGGAAGAAGAGAGGCGAG
AAAGATGAAACTGAGTATGAGTACAGTGGGAGTGAGGAAGAAGAGGAGGAAGTGCCTGAACAGGAAGGAGAGCCAAGTTCCATTGTGAAC
GTGCCTGGTGAGTCTACTCTTCGCCGAGATTTCCTGAGACTGCAGCAGGAGAACAAGGAACGTTCCGAGGCTCTTCGGAGACAACAGTTA
CTACAGGAGCAACAGCTCCGGGAGCAGGAAGAATATAAAAGGCAACTGCTGGCAGAGAGACAGAAGCGGATTGAGCAGCAGAAAGAACAG
AGGCGACGGCTAGAAGAGCAACAAAGGAGAGAGCGGGAAGCTAGAAGGCAGCAGGAACGTGAACAGCGAAGGAGAGAACAAGAAGAAAAG
AGGCGTCTAGAGGAGTTGGAGAGAAGGCGCAAAGAAGAAGAGGAGAGGAGACGGGCAGAAGAAGAAAAGAGGAGAGTTGAAAGAGAACAG
GAGTATATCAGGCGACAGCTAGAAGAGGAGCAGCGGCACTTGGAAGTCCTTCAGCAGCAGCTGCTCCAGGAGCAGGCCATGTTACTGCAT
GACCATAGGAGGCCGCACCCGCAGCACTCGCAGCAGCCGCCACCACCGCAGCAGGAAAGGAGCAAGCCAAGCTTCCATGCTCCCGAGCCC
AAAGCCCACTACGAGCCTGCTGACCGAGCGCGAGAGGTTCCTGTGAGAACAACATCTCGCTCCCCTGTTCTGTCCCGTCGAGATTCCCCA
CTGCAGGGCAGTGGGCAGCAGAATAGCCAGGCAGGACAGAGAAACTCCACCAGTATTGAGCCCAGGCTTCTGTGGGAGAGAGTGGAGAAG
CTGGTGCCCAGACCTGGCAGTGGCAGCTCCTCAGGGTCCAGCAACTCAGGATCCCAGCCCGGGTCTCACCCTGGGTCTCAGAGTGGCTCC
GGGGAACGCTTCAGAGTGAGATCATCATCCAAGTCTGAAGGCTCTCCATCTCAGCGCCTGGAAAATGCAGTGAAAAAACCTGAAGATAAA
AAGGAAGTTTTCAGACCCCTCAAGCCTGCTGATCTGACCGCACTGGCCAAAGAGCTTCGAGCAGTGGAAGATGTACGGCCACCTCACAAA
GTAACGGACTACTCCTCATCCAGTGAGGAGTCGGGGACGACGGATGAGGAGGACGACGATGTGGAGCAGGAAGGGGCTGACGAGTCCACC
TCAGGACCAGAGGACACCAGAGCAGCGTCATCTCTGAATTTGAGCAATGGTGAAACGGAATCTGTGAAAACCATGATTGTCCATGATGAT
GTAGAAAGTGAGCCGGCCATGACCCCATCCAAGGAGGGCACTCTAATCGTCCGCCAGACTCAGTCCGCTAGTAGCACACTCCAGAAACAC
AAATCTTCCTCCTCCTTTACACCTTTTATAGACCCCAGATTACTACAGATTTCTCCATCTAGCGGAACAACAGTGACATCTGTGGTGGGA
TTTTCCTGTGATGGGATGAGACCAGAAGCCATAAGGCAAGATCCTACCCGGAAAGGCTCAGTGGTCAATGTGAATCCTACCAACACTAGG
CCACAGAGTGACACCCCGGAGATTCGTAAATACAAGAAGAGGTTTAACTCTGAGATTCTGTGTGCTGCCTTATGGGGAGTGAATTTGCTA
GTGGGTACAGAGAGTGGCCTGATGCTGCTGGACAGAAGTGGCCAAGGGAAGGTCTATCCTCTTATCAACCGAAGACGATTTCAACAAATG
GACGTACTTGAGGGCTTGAATGTCTTGGTGACAATATCTGGCAAAAAGGATAAGTTACGTGTCTACTATTTGTCCTGGTTAAGAAATAAA
ATACTTCACAATGATCCAGAAGTTGAGAAGAAGCAGGGATGGACAACCGTAGGGGATTTGGAAGGATGTGTACATTATAAAGTTGTAAAA
TATGAAAGAATCAAATTTCTGGTGATTGCTTTGAAGAGTTCTGTGGAAGTCTATGCGTGGGCACCAAAGCCATATCACAAATTTATGGCC
TTTAAGTCATTTGGAGAATTGGTACATAAGCCATTACTGGTGGATCTCACTGTTGAGGAAGGCCAGAGGTTGAAAGTGATCTATGGATCC
TGTGCTGGATTCCATGCTGTTGATGTGGATTCAGGATCAGTCTATGACATTTATCTACCAACACATATCCAGTGTAGCATCAAACCCCAT
GCAATCATCATCCTCCCCAATACAGATGGAATGGAGCTTCTGGTGTGCTATGAAGATGAGGGGGTTTATGTAAACACATATGGAAGGATC
ACCAAGGATGTAGTTCTACAGTGGGGAGAGATGCCTACATCAGTAGCATATATTCGATCCAATCAGACAATGGGCTGGGGAGAGAAGGCC
ATAGAGATCCGATCTGTGGAAACTGGTCACTTGGATGGTGTGTTCATGCACAAAAGGGCTCAAAGACTAAAATTCTTGTGTGAACGCAAT
GACAAGGTGTTCTTTGCCTCTGTTCGGTCTGGTGGCAGCAGTCAGGTTTATTTCATGACCTTAGGCAGGACTTCTCTTCTGAGCTGGTAG
AAGCAGTGTGATCCAGGGATTACTGGCCTCCAGAGTCTTCAAGATCCTGAGAACTTGGAATTCCTTGTAACTGGAGCTCGGAGCTGCACC
GAGGGCAACCAGGACAGCTGTGTGTGCAGACCTCATGTGTTGGGTTCTCTCCCCTCCTTCCTGTTCCTCTTATATACCAGTTTATCCCCA
TTCTTTTTTTTTTTCTTACTCCAAAATAAATCAAGGCTGCAATGCAGCTGGTGCTGTTCAGATTCTACCATCAGGTGCTATAAGTGTTTG
GGATTGAGCATCATACTGGAAAGCAAACACCTTTCCTCCAGCTCCAGAATTCCTTGTCTCTGAATGACTCTGTCTTGTGGGTGTCTGACA
GTGGCGACGATGAACATGCCGTTGGTTTTATTGGCAGTGGGCACAAGGAGGTGAGAAGTGGTGGTAAAAGGAGCGGAGTGCTGAAGCAGA
GAGCAGATTTAATATAGTAACATTAACAGTGTATTTAATTGACATTTCTTTTTTGTAATGTGACGATATGTGGACAAAGAAGAAGATGCA
GGTTTAAGAAGTTAATATTTATAAAATGTGAAAGACACAGTTACTAGGATAACTTTTTTGTGGGTGGGGCTTGGGAGATGGGGTGGGGTG
GGTTAAGGGGTCCCATTTTGTTTCTTTGGATTTGGGGTGGGGGTCCTGGCCAAGAACTCAGTCATTTTTCTGTGTACCAGGTTGCCTAAA
TCATGTGCAGATGGTTCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAGGAAAAAAAAAAAGAAAAAGAAAACGTGTGCATTTTGTATAATG
GCCAGAACTTTGTCGTGTGACAGTATTAGCACTGCCTCAGTTAAAGGTTTAATTTTTGTTTAAACCTAGACGTGCAACAAAAGTTTTACC
ACAGTCTGCACTTGCAGAAGAAAGAAAAAAATTCAAACCACATGTTTATTTTTTTTTTGCCTACCTCATTGTTCTTAATGCATTGAGAGG
TGATTTAGTTTATATGTTTTTGGAAGAAACCATTAATGTTTAATTTAATCTTAATACCAAAACGACCAGATTGAAGTTTGACTTTTATTG
TCACAAATCAGCAGGCACAAGAACTGTCCATGAAGATGGGAAATAGCCTTAAGGCTGATGCAGTTTACTTACAAGTTTAGAAACCAGAAT
GCTTTGTTTTTACCAGATTCACCATTAGAGGTTGATGGGGCAACTGCAGCCCATGACACAAGATCTCATTGTTCTCGATGTAGAGGGGTT
GGTAGCAGACAGGTGGTTACATTAGAATAGTCACACAAACTGTTCAGTGTTGCAGGAACCTTTTCTTGGGGGTGGGGGAGTTTCCCTTTT
CTAAAAATGCAATGCACTAAAACTATTTTAAGAATGTAGTTAATTCTGCTTATTCATAAAGTGGGCATCTTCTGTGTTTTAGGTGTAATA
TCGAAGTCCTGGCTTTTCTCGTTTTCTCACTTGCTCTCTTGTTCTCTGTTTTTTTAAACCAATTTTACTTTATGAATATATTCATGACAT
TTGTAATAAATGTCTTGAGAAAGAATTTGTTTCATGGCTTCATGGTCATCACTCAAGCTCCCGTAAGGATATTACCGTCTCAGGAAAGGA
TCAGGACTCCATGTCACAGTCCTGCCATCTTACTTTCCTCTTGTCGAGTTCTGAGTGGAAATAACTGCATTATGGCTGCTTTAACCTCAG
TCATCAAAAGAAACTTGCTGTTTTTTAGGCTTGATCTTTTTCCTTTGTGGTTAATTTTCCTGTATATTGTGAAAATGGGGGATTTTCCCT
CTGCTCCCACCCACCTAAACACAGCAGCCATTTGTACCTGTTTGCTTCCCATCCCACTTGGCACCCACTCTGACCTCTTGTCAGTTTCCT
GTTCCTGGTTCCATCTTTTTGAAAAAGGCCCTCCTTTGAGCTACAAACATCTGGTAAGACAAGTACATCCACTCATGAATGCAGACACAG
CAGCTGGTGGTTTTGTGTATACCTGTAAAGACAAGCTGAGAAGCTTACTTTTTGGGGAAGTAAAAGAAGATGGAAATGGATGTTTCATTT
GTATGAGTTTGGAGCAGTGCTGAAGGCCAAAGCCGCCTACTGGTTTGTAGTTAACCTAGAGAAGGTTGAAAAATTAATCCTACCTTTAAA
GGGATTTGAGGTAGGCTGGATTCCATCGCCACAGGACTTTAGTTAGAATTAAATTCCTGCTTGTAATTTATATCCATGTTTAGGCTTTTC
ATAAGATGAAACATGCCACAGTGAACACACTCGTGTACATATCAAGAGAAGAAGGAAAGGCACAGGTGGAGAACAGTAAAAGGTGGGCAG
ATGTCTTTGAAGAAATGCTCAATGTCTGATGCTAAGTGGGAGAAGGCAGAGAACAAAGGATGTGGCATAATGGTCTTAACATTATCCAAA
GACTTGAAGCTCCATGTCTGTAAGTCAAATGTTACACAAAAAAAAATGCAAATGGTGTTTCATTGGAATTACCAAGTGCTTAGAACTTGC
TGGCTTTCCCATAGGTGGTAAAGGGGTCTGAGCTCACACCGAGTTGTGCTTGGCTTGCTTGTGCAGCTCCAGGCACCCGGTGGGCACTCT
GGTGGTGTTTGTGGTGAACTGAATTGAATCCATTGTTGGGCTTAAGTTACTGAAATTGGAACACCCTTTGTCCTTCTCGGCGGGGGCTTC
CTGGTCTGTGCTTTACTTGGCTTTTTTCCTTCCCGTCTTAGCCTCACCCCCTTGTCAACCAGATTGAGTTGCTATAGCTTGATGCAGGGA
CCCAGTGAAGTTTCTCCGTTAAAGATTGGGAGTCGTCGAAATGTTTAGATTCTTTTAGGAAAGGAATTATTTTCCCCCCTTTTACAGGGT
AGTAACTTCTCCACAGAAGTGCCAATATGGCAAAATTACACAAGAAAACAGTATTGCAATGACACCATTACATAAGGAACATTGAACTGT
TAGAGGAGTGCTCTTCCAAACAAAACAAAAATGTCTCTAGGTTTAGTCAGAGCTTTCACAAGTAATAACCTTTCTGTATTAAAATCAGAG
TAACCCTTTCTGTATTGAGTGCAGTGTTTTTTACTCTTTTCTCATGCACATGTTACGTTGGAGAAAATGTTTACAAAAATGGTTTTGTTA
CACTAATGCGCACCACATATTTATGGTTTATTTTAAGTGACTTTTTATGGGTTATTTAGGTTTTCGTCTTAGTTGTAGCACACTTACCCT
AATTTTGCCAATTATTAATTTGCTAAATAGTAATACAAATGACAAACTGCATTAAATTTACTAATTATAAAAGCTGCAAAGCAGACTGGT
GGCAAGTACACAGCCCTTTTTTTTGCAGTGCTAACTTGTCTACTGTGTATTATGAAAATTACTGTTGTCCCCCCACCCTTTTTTCCTTAA

>In-frame_ENST00000295317_ENST00000413150_TCGA-24-1553-01A_RNF149_chr2_101910476_-_MAP4K4_chr2_102407182_length(amino acids)=1384AA_start in transcript=75_stop in transcript=4229
MTPPSQLSGAAMAWRRREASVGARGVLALALLALALCVPGARGRALEWFSAVVNIEYVDPQTNLTVWSVSESGRFGDSSPKEGAHGLVGV
PWAPGGDLEGCAPDTRFFVPEPGGRGAAPWVALVARGGCTFKDKVLVAARRNASAVVLYNEERYGNITLPMSHAGTGNIVVIMISYPKGR
EILELVQKGIPVTMTIGVGTRHVQEFISGQSVVFVAIAFITMMIISLAWLIFYYIQRFLYTGSQIGSQSHRKETKKVIGQLLLHTVKHGE
KGRHVKTGQLAAIKVMDVTEDEEEEIKLEINMLKKYSHHRNIATYYGAFIKKSPPGHDDQLWLVMEFCGAGSITDLVKNTKGNTLKEDWI
AYISREILRGLAHLHIHHVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDLWSCG
ITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFFSFIEGCLVKNYMQRPSTEQLLKHPFIRDQPNERQVRIQLKDHIDRTR
KKRGEKDETEYEYSGSEEEEEEVPEQEGEPSSIVNVPGESTLRRDFLRLQQENKERSEALRRQQLLQEQQLREQEEYKRQLLAERQKRIE
QQKEQRRRLEEQQRREREARRQQEREQRRREQEEKRRLEELERRRKEEEERRRAEEEKRRVEREQEYIRRQLEEEQRHLEVLQQQLLQEQ
AMLLHDHRRPHPQHSQQPPPPQQERSKPSFHAPEPKAHYEPADRAREVPVRTTSRSPVLSRRDSPLQGSGQQNSQAGQRNSTSIEPRLLW
ERVEKLVPRPGSGSSSGSSNSGSQPGSHPGSQSGSGERFRVRSSSKSEGSPSQRLENAVKKPEDKKEVFRPLKPADLTALAKELRAVEDV
RPPHKVTDYSSSSEESGTTDEEDDDVEQEGADESTSGPEDTRAASSLNLSNGETESVKTMIVHDDVESEPAMTPSKEGTLIVRQTQSASS
TLQKHKSSSSFTPFIDPRLLQISPSSGTTVTSVVGFSCDGMRPEAIRQDPTRKGSVVNVNPTNTRPQSDTPEIRKYKKRFNSEILCAALW
GVNLLVGTESGLMLLDRSGQGKVYPLINRRRFQQMDVLEGLNVLVTISGKKDKLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDLEGCVH
YKVVKYERIKFLVIALKSSVEVYAWAPKPYHKFMAFKSFGELVHKPLLVDLTVEEGQRLKVIYGSCAGFHAVDVDSGSVYDIYLPTHIQC
SIKPHAIIILPNTDGMELLVCYEDEGVYVNTYGRITKDVVLQWGEMPTSVAYIRSNQTMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKF

--------------------------------------------------------------
>In-frame_ENST00000295317_ENST00000347699_TCGA-24-1553-01A_RNF149_chr2_101910476_-_MAP4K4_chr2_102407182_length(transcript)=4557nt_BP=888nt
CGCCTCAGTCTTCCAGGGCGGCGGTGGGTGTCCGCTTCTCTCTGCTCTTCGACTGCACCGCACTCGCGCGTGACCCTGACTCCCCCTAGT
CAGCTCAGCGGTGCTGCCATGGCGTGGCGGCGGCGCGAAGCCAGCGTCGGGGCTCGCGGCGTGTTGGCTCTGGCGTTGCTCGCCCTGGCC
CTGTGCGTGCCCGGGGCCCGGGGCCGGGCTCTCGAGTGGTTCTCGGCCGTGGTAAACATCGAGTACGTGGACCCGCAGACCAACCTGACG
GTGTGGAGCGTCTCGGAGAGTGGCCGCTTCGGCGACAGCTCGCCCAAGGAGGGCGCGCATGGCCTGGTGGGCGTCCCGTGGGCGCCCGGC
GGAGACCTCGAGGGCTGCGCGCCCGACACGCGCTTCTTCGTGCCCGAGCCCGGCGGCCGAGGGGCCGCGCCCTGGGTCGCCCTGGTGGCT
CGTGGGGGCTGCACCTTCAAGGACAAGGTGCTGGTGGCGGCGCGGAGGAACGCCTCGGCCGTCGTCCTCTACAATGAGGAGCGCTACGGG
AACATCACCTTGCCCATGTCTCACGCGGGAACAGGAAATATAGTGGTCATTATGATTAGCTATCCAAAAGGAAGAGAAATTTTGGAGCTG
GTGCAAAAAGGAATTCCAGTAACGATGACCATAGGGGTTGGCACCCGGCATGTACAGGAGTTCATCAGCGGTCAGTCTGTGGTGTTTGTG
GCCATTGCCTTCATCACCATGATGATTATCTCGTTAGCCTGGCTAATATTTTACTATATACAGCGTTTCCTATATACTGGCTCTCAGATT
GGAAGTCAGAGCCATAGAAAAGAAACTAAGAAAGTTATTGGCCAGCTTCTACTTCATACTGTAAAGCATGGAGAAAAGGGTCGACATGTT
AAAACGGGTCAGTTGGCAGCCATCAAAGTTATGGATGTCACTGAGGATGAAGAGGAAGAAATCAAACTGGAGATAAATATGCTAAAGAAA
TACTCTCATCACAGAAACATTGCAACATATTATGGTGCTTTCATCAAAAAGAGCCCTCCAGGACATGATGACCAACTCTGGCTTGTTATG
GAGTTCTGTGGGGCTGGGTCCATTACAGACCTTGTGAAGAACACCAAAGGGAACACACTCAAAGAAGACTGGATCGCTTACATCTCCAGA
GAAATCCTGAGGGGACTGGCACATCTTCACATTCATCATGTGATTCACCGGGATATCAAGGGCCAGAATGTGTTGCTGACTGAGAATGCA
GAGGTGAAACTTGTTGACTTTGGTGTGAGTGCTCAGCTGGACAGGACTGTGGGGCGGAGAAATACGTTCATAGGCACTCCCTACTGGATG
GCTCCTGAGGTCATCGCCTGTGATGAGAACCCAGATGCCACCTATGATTACAGAAGTGATCTTTGGTCTTGTGGCATTACAGCCATTGAG
ATGGCAGAAGGTGCTCCCCCTCTCTGTGACATGCATCCAATGAGAGCACTGTTTCTCATTCCCAGAAACCCTCCTCCCCGGCTGAAGTCA
AAAAAATGGTCGAAGAAGTTTTTTAGTTTTATAGAAGGGTGCCTGGTGAAGAATTACATGCAGCGGCCCTCTACAGAGCAGCTTTTGAAA
CATCCTTTTATAAGGGATCAGCCAAATGAAAGGCAAGTTAGAATCCAGCTTAAGGATCATATAGATCGTACCAGGAAGAAGAGAGGCGAG
AAAGATGAAACTGAGTATGAGTACAGTGGGAGTGAGGAAGAAGAGGAGGAAGTGCCTGAACAGGAAGGAGAGCCAAGTTCCATTGTGAAC
GTGCCTGGTGAGTCTACTCTTCGCCGAGATTTCCTGAGACTGCAGCAGGAGAACAAGGAACGTTCCGAGGCTCTTCGGAGACAACAGTTA
CTACAGGAGCAACAGCTCCGGGAGCAGGAAGAATATAAAAGGCAACTGCTGGCAGAGAGACAGAAGCGGATTGAGCAGCAGAAAGAACAG
AGGCGACGGCTAGAAGAGCAACAAAGGAGAGAGCGGGAAGCTAGAAGGCAGCAGGAACGTGAACAGCGAAGGAGAGAACAAGAAGAAAAG
AGGCGTCTAGAGGAGTTGGAGAGAAGGCGCAAAGAAGAAGAGGAGAGGAGACGGGCAGAAGAAGAAAAGAGGAGAGTTGAAAGAGAACAG
GAGTATATCAGGCGACAGCTAGAAGAGGAGCAGCGGCACTTGGAAGTCCTTCAGCAGCAGCTGCTCCAGGAGCAGGCCATGTTACTGGAG
TGCCGATGGCGGGAGATGGAGGAGCACCGGCAGGCAGAGAGGCTCCAGAGGCAGTTGCAACAAGAACAAGCATATCTCCTGTCTCTACAG
CATGACCATAGGAGGCCGCACCCGCAGCACTCGCAGCAGCCGCCACCACCGCAGCAGGAAAGGAGCAAGCCAAGCTTCCATGCTCCCGAG
CCCAAAGCCCACTACGAGCCTGCTGACCGAGCGCGAGAGGTGGAAGATAGATTTAGGAAAACTAACCACAGCTCCCCTGAAGCCCAGTCT
AAGCAGACAGGCAGAGTATTGGAGCCACCAGTGCCTTCCCGATCAGAGTCTTTTTCCAATGGCAACTCCGAGTCTGTGCATCCCGCCCTG
CAGAGACCAGCGGAGCCACAGGTTCCTGTGAGAACAACATCTCGCTCCCCTGTTCTGTCCCGTCGAGATTCCCCACTGCAGGGCAGTGGG
CAGCAGAATAGCCAGGCAGGACAGAGAAACTCCACCAGTATTGAGCCCAGGCTTCTGTGGGAGAGAGTGGAGAAGCTGGTGCCCAGACCT
GGCAGTGGCAGCTCCTCAGGGTCCAGCAACTCAGGATCCCAGCCCGGGTCTCACCCTGGGTCTCAGAGTGGCTCCGGGGAACGCTTCAGA
GTGAGATCATCATCCAAGTCTGAAGGCTCTCCATCTCAGCGCCTGGAAAATGCAGTGAAAAAACCTGAAGATAAAAAGGAAGTTTTCAGA
CCCCTCAAGCCTGCTGATCTGACCGCACTGGCCAAAGAGCTTCGAGCAGTGGAAGATGTACGGCCACCTCACAAAGTAACGGACTACTCC
TCATCCAGTGAGGAGTCGGGGACGACGGATGAGGAGGACGACGATGTGGAGCAGGAAGGGGCTGACGAGTCCACCTCAGGACCAGAGGAC
ACCAGAGCAGCGTCATCTCTGAATTTGAGCAATGGTGAAACGGAATCTGTGAAAACCATGATTGTCCATGATGATGTAGAAAGTGAGCCG
GCCATGACCCCATCCAAGGAGGGCACTCTAATCGTCCGCCAGACTCAGTCCGCTAGTAGCACACTCCAGAAACACAAATCTTCCTCCTCC
TTTACACCTTTTATAGACCCCAGATTACTACAGATTTCTCCATCTAGCGGAACAACAGTGACATCTGTGGTGGGATTTTCCTGTGATGGG
ATGAGACCAGAAGCCATAAGGCAAGATCCTACCCGGAAAGGCTCAGTGGTCAATGTGAATCCTACCAACACTAGGCCACAGAGTGACACC
CCGGAGATTCGTAAATACAAGAAGAGGTTTAACTCTGAGATTCTGTGTGCTGCCTTATGGGGAGTGAATTTGCTAGTGGGTACAGAGAGT
GGCCTGATGCTGCTGGACAGAAGTGGCCAAGGGAAGGTCTATCCTCTTATCAACCGAAGACGATTTCAACAAATGGACGTACTTGAGGGC
TTGAATGTCTTGGTGACAATATCTGGCAAAAAGGATAAGTTACGTGTCTACTATTTGTCCTGGTTAAGAAATAAAATACTTCACAATGAT
CCAGAAGTTGAGAAGAAGCAGGGATGGACAACCGTAGGGGATTTGGAAGGATGTGTACATTATAAAGTTGTAAAATATGAAAGAATCAAA
TTTCTGGTGATTGCTTTGAAGAGTTCTGTGGAAGTCTATGCGTGGGCACCAAAGCCATATCACAAATTTATGGCCTTTAAGTCATTTGGA
GAATTGGTACATAAGCCATTACTGGTGGATCTCACTGTTGAGGAAGGCCAGAGGTTGAAAGTGATCTATGGATCCTGTGCTGGATTCCAT
GCTGTTGATGTGGATTCAGGATCAGTCTATGACATTTATCTACCAACACATATCCAGTGTAGCATCAAACCCCATGCAATCATCATCCTC
CCCAATACAGATGGAATGGAGCTTCTGGTGTGCTATGAAGATGAGGGGGTTTATGTAAACACATATGGAAGGATCACCAAGGATGTAGTT
CTACAGTGGGGAGAGATGCCTACATCAGTAGCATATATTCGATCCAATCAGACAATGGGCTGGGGAGAGAAGGCCATAGAGATCCGATCT
GTGGAAACTGGTCACTTGGATGGTGTGTTCATGCACAAAAGGGCTCAAAGACTAAAATTCTTGTGTGAACGCAATGACAAGGTGTTCTTT
GCCTCTGTTCGGTCTGGTGGCAGCAGTCAGGTTTATTTCATGACCTTAGGCAGGACTTCTCTTCTGAGCTGGTAGAAGCAGTGTGATCCA

>In-frame_ENST00000295317_ENST00000347699_TCGA-24-1553-01A_RNF149_chr2_101910476_-_MAP4K4_chr2_102407182_length(amino acids)=1469AA_start in transcript=75_stop in transcript=4484
MTPPSQLSGAAMAWRRREASVGARGVLALALLALALCVPGARGRALEWFSAVVNIEYVDPQTNLTVWSVSESGRFGDSSPKEGAHGLVGV
PWAPGGDLEGCAPDTRFFVPEPGGRGAAPWVALVARGGCTFKDKVLVAARRNASAVVLYNEERYGNITLPMSHAGTGNIVVIMISYPKGR
EILELVQKGIPVTMTIGVGTRHVQEFISGQSVVFVAIAFITMMIISLAWLIFYYIQRFLYTGSQIGSQSHRKETKKVIGQLLLHTVKHGE
KGRHVKTGQLAAIKVMDVTEDEEEEIKLEINMLKKYSHHRNIATYYGAFIKKSPPGHDDQLWLVMEFCGAGSITDLVKNTKGNTLKEDWI
AYISREILRGLAHLHIHHVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDLWSCG
ITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFFSFIEGCLVKNYMQRPSTEQLLKHPFIRDQPNERQVRIQLKDHIDRTR
KKRGEKDETEYEYSGSEEEEEEVPEQEGEPSSIVNVPGESTLRRDFLRLQQENKERSEALRRQQLLQEQQLREQEEYKRQLLAERQKRIE
QQKEQRRRLEEQQRREREARRQQEREQRRREQEEKRRLEELERRRKEEEERRRAEEEKRRVEREQEYIRRQLEEEQRHLEVLQQQLLQEQ
AMLLECRWREMEEHRQAERLQRQLQQEQAYLLSLQHDHRRPHPQHSQQPPPPQQERSKPSFHAPEPKAHYEPADRAREVEDRFRKTNHSS
PEAQSKQTGRVLEPPVPSRSESFSNGNSESVHPALQRPAEPQVPVRTTSRSPVLSRRDSPLQGSGQQNSQAGQRNSTSIEPRLLWERVEK
LVPRPGSGSSSGSSNSGSQPGSHPGSQSGSGERFRVRSSSKSEGSPSQRLENAVKKPEDKKEVFRPLKPADLTALAKELRAVEDVRPPHK
VTDYSSSSEESGTTDEEDDDVEQEGADESTSGPEDTRAASSLNLSNGETESVKTMIVHDDVESEPAMTPSKEGTLIVRQTQSASSTLQKH
KSSSSFTPFIDPRLLQISPSSGTTVTSVVGFSCDGMRPEAIRQDPTRKGSVVNVNPTNTRPQSDTPEIRKYKKRFNSEILCAALWGVNLL
VGTESGLMLLDRSGQGKVYPLINRRRFQQMDVLEGLNVLVTISGKKDKLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDLEGCVHYKVVK
YERIKFLVIALKSSVEVYAWAPKPYHKFMAFKSFGELVHKPLLVDLTVEEGQRLKVIYGSCAGFHAVDVDSGSVYDIYLPTHIQCSIKPH
AIIILPNTDGMELLVCYEDEGVYVNTYGRITKDVVLQWGEMPTSVAYIRSNQTMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERN

--------------------------------------------------------------
>In-frame_ENST00000295317_ENST00000456652_TCGA-24-1553-01A_RNF149_chr2_101910476_-_MAP4K4_chr2_102407182_length(transcript)=4620nt_BP=888nt
CGCCTCAGTCTTCCAGGGCGGCGGTGGGTGTCCGCTTCTCTCTGCTCTTCGACTGCACCGCACTCGCGCGTGACCCTGACTCCCCCTAGT
CAGCTCAGCGGTGCTGCCATGGCGTGGCGGCGGCGCGAAGCCAGCGTCGGGGCTCGCGGCGTGTTGGCTCTGGCGTTGCTCGCCCTGGCC
CTGTGCGTGCCCGGGGCCCGGGGCCGGGCTCTCGAGTGGTTCTCGGCCGTGGTAAACATCGAGTACGTGGACCCGCAGACCAACCTGACG
GTGTGGAGCGTCTCGGAGAGTGGCCGCTTCGGCGACAGCTCGCCCAAGGAGGGCGCGCATGGCCTGGTGGGCGTCCCGTGGGCGCCCGGC
GGAGACCTCGAGGGCTGCGCGCCCGACACGCGCTTCTTCGTGCCCGAGCCCGGCGGCCGAGGGGCCGCGCCCTGGGTCGCCCTGGTGGCT
CGTGGGGGCTGCACCTTCAAGGACAAGGTGCTGGTGGCGGCGCGGAGGAACGCCTCGGCCGTCGTCCTCTACAATGAGGAGCGCTACGGG
AACATCACCTTGCCCATGTCTCACGCGGGAACAGGAAATATAGTGGTCATTATGATTAGCTATCCAAAAGGAAGAGAAATTTTGGAGCTG
GTGCAAAAAGGAATTCCAGTAACGATGACCATAGGGGTTGGCACCCGGCATGTACAGGAGTTCATCAGCGGTCAGTCTGTGGTGTTTGTG
GCCATTGCCTTCATCACCATGATGATTATCTCGTTAGCCTGGCTAATATTTTACTATATACAGCGTTTCCTATATACTGGCTCTCAGATT
GGAAGTCAGAGCCATAGAAAAGAAACTAAGAAAGTTATTGGCCAGCTTCTACTTCATACTGTAAAGCATGGAGAAAAGGGTCGACATGTT
AAAACGGGTCAGTTGGCAGCCATCAAAGTTATGGATGTCACTGAGGATGAAGAGGAAGAAATCAAACTGGAGATAAATATGCTAAAGAAA
TACTCTCATCACAGAAACATTGCAACATATTATGGTGCTTTCATCAAAAAGAGCCCTCCAGGACATGATGACCAACTCTGGCTTGTTATG
GAGTTCTGTGGGGCTGGGTCCATTACAGACCTTGTGAAGAACACCAAAGGGAACACACTCAAAGAAGACTGGATCGCTTACATCTCCAGA
GAAATCCTGAGGGGACTGGCACATCTTCACATTCATCATGTGATTCACCGGGATATCAAGGGCCAGAATGTGTTGCTGACTGAGAATGCA
GAGGTGAAACTTGATGAAACTGAGTATGAGTACAGTGGGAGTGAGGAAGAAGAGGAGGAAGTGCCTGAACAGGAAGGAGAGCCAAGTTCC
ATTGTGAACGTGCCTGGTGAGTCTACTCTTCGCCGAGATTTCCTGAGACTGCAGCAGGAGAACAAGGAACGTTCCGAGGCTCTTCGGAGA
CAACAGTTACTACAGGAGCAACAGCTCCGGGAGCAGGAAGAATATAAAAGGCAACTGCTGGCAGAGAGACAGAAGCGGATTGAGCAGCAG
AAAGAACAGAGGCGACGGCTAGAAGAGCAACAAAGGAGAGAGCGGGAAGCTAGAAGGCAGCAGGAACGTGAACAGCGAAGGAGAGAACAA
GAAGAAAAGAGGCGTCTAGAGGAGTTGGAGAGAAGGCGCAAAGAAGAAGAGGAGAGGAGACGGGCAGAAGAAGAAAAGAGGAGAGTTGAA
AGAGAACAGGAGTATATCAGGCGACAGCTAGAAGAGGAGCAGCGGCACTTGGAAGTCCTTCAGCAGCAGCTGCTCCAGGAGCAGGCCATG
TTACTGGAGTGCCGATGGCGGGAGATGGAGGAGCACCGGCAGGCAGAGAGGCTCCAGAGGCAGTTGCAACAAGAACAAGCATATCTCCTG
TCTCTACAGCATGACCATAGGAGGCCGCACCCGCAGCACTCGCAGCAGCCGCCACCACCGCAGCAGGAAAGGAGCAAGCCAAGCTTCCAT
GCTCCCGAGCCCAAAGCCCACTACGAGCCTGCTGACCGAGCGCGAGAGGTTCCTGTGAGAACAACATCTCGCTCCCCTGTTCTGTCCCGT
CGAGATTCCCCACTGCAGGGCAGTGGGCAGCAGAATAGCCAGGCAGGACAGAGAAACTCCACCAGTATTGAGCCCAGGCTTCTGTGGGAG
AGAGTGGAGAAGCTGGTGCCCAGACCTGGCAGTGGCAGCTCCTCAGGGTCCAGCAACTCAGGATCCCAGCCCGGGTCTCACCCTGGGTCT
CAGAGTGGCTCCGGGGAACGCTTCAGAGTGAGATCATCATCCAAGTCTGAAGGCTCTCCATCTCAGCGCCTGGAAAATGCAGTGAAAAAA
CCTGAAGATAAAAAGGAAGTTTTCAGACCCCTCAAGCCTGCTGATCTGACCGCACTGGCCAAAGAGCTTCGAGCAGTGGAAGATGTACGG
CCACCTCACAAAGTAACGGACTACTCCTCATCCAGTGAGGAGTCGGGGACGACGGATGAGGAGGACGACGATGTGGAGCAGGAAGGGGCT
GACGAGTCCACCTCAGGACCAGAGGACACCAGAGCAGCGTCATCTCTGAATTTGAGCAATGGTGAAACGGAATCTGTGAAAACCATGATT
GTCCATGATGATGTAGAAAGTGAGCCGGCCATGACCCCATCCAAGGAGGGCACTCTAATCGTCCGCCAGACTCAGTCCGCTAGTAGCACA
CTCCAGAAACACAAATCTTCCTCCTCCTTTACACCTTTTATAGACCCCAGATTACTACAGATTTCTCCATCTAGCGGAACAACAGTGACA
TCTGTGGTGGGATTTTCCTGTGATGGGATGAGACCAGAAGCCATAAGGCAAGATCCTACCCGGAAAGGCTCAGTGGTCAATGTGAATCCT
ACCAACACTAGGCCACAGAGTGACACCCCGGAGATTCGTAAATACAAGAAGAGGTTTAACTCTGAGATTCTGTGTGCTGCCTTATGGGGA
GTGAATTTGCTAGTGGGTACAGAGAGTGGCCTGATGCTGCTGGACAGAAGTGGCCAAGGGAAGGTCTATCCTCTTATCAACCGAAGACGA
TTTCAACAAATGGACGTACTTGAGGGCTTGAATGTCTTGGTGACAATATCTGGCAAAAAGGATAAGTTACGTGTCTACTATTTGTCCTGG
TTAAGAAATAAAATACTTCACAATGATCCAGAAGTTGAGAAGAAGCAGGGATGGACAACCGTAGGGGATTTGGAAGGATGTGTACATTAT
AAAGTTGTAAAATATGAAAGAATCAAATTTCTGGTGATTGCTTTGAAGAGTTCTGTGGAAGTCTATGCGTGGGCACCAAAGCCATATCAC
AAATTTATGGCCTTTAAGTCATTTGGAGAATTGGTACATAAGCCATTACTGGTGGATCTCACTGTTGAGGAAGGCCAGAGGTTGAAAGTG
ATCTATGGATCCTGTGCTGGATTCCATGCTGTTGATGTGGATTCAGGATCAGTCTATGACATTTATCTACCAACACATATCCAGTGTAGC
ATCAAACCCCATGCAATCATCATCCTCCCCAATACAGATGGAATGGAGCTTCTGGTGTGCTATGAAGATGAGGGGGTTTATGTAAACACA
TATGGAAGGATCACCAAGGATGTAGTTCTACAGTGGGGAGAGATGCCTACATCAGTAGCATATATTCGATCCAATCAGACAATGGGCTGG
GGAGAGAAGGCCATAGAGATCCGATCTGTGGAAACTGGTCACTTGGATGGTGTGTTCATGCACAAAAGGGCTCAAAGACTAAAATTCTTG
TGTGAACGCAATGACAAGGTGTTCTTTGCCTCTGTTCGGTCTGGTGGCAGCAGTCAGGTTTATTTCATGACCTTAGGCAGGACTTCTCTT
CTGAGCTGGTAGAAGCAGTGTGATCCAGGGATTACTGGCCTCCAGAGTCTTCAAGATCCTGAGAACTTGGAATTCCTTGTAACTGGAGCT
CGGAGCTGCACCGAGGGCAACCAGGACAGCTGTGTGTGCAGACCTCATGTGTTGGGTTCTCTCCCCTCCTTCCTGTTCCTCTTATATACC
AGTTTATCCCCATTCTTTTTTTTTTTCTTACTCCAAAATAAATCAAGGCTGCAATGCAGCTGGTGCTGTTCAGATTCTACCATCAGGTGC
TATAAGTGTTTGGGATTGAGCATCATACTGGAAAGCAAACACCTTTCCTCCAGCTCCAGAATTCCTTGTCTCTGAATGACTCTGTCTTGT
GGGTGTCTGACAGTGGCGACGATGAACATGCCGTTGGTTTTATTGGCAGTGGGCACAAGGAGGTGAGAAGTGGTGGTAAAAGGAGCGGAG
TGCTGAAGCAGAGAGCAGATTTAATATAGTAACATTAACAGTGTATTTAATTGACATTTCTTTTTTGTAATGTGACGATATGTGGACAAA
GAAGAAGATGCAGGTTTAAGAAGTTAATATTTATAAAATGTGAAAGACACAGTTACTAGGATAACTTTTTTGTGGGTGGGGCTTGGGAGA
TGGGGTGGGGTGGGTTAAGGGGTCCCATTTTGTTTCTTTGGATTTGGGGTGGGGGTCCTGGCCAAGAACTCAGTCATTTTTCTGTGTACC

>In-frame_ENST00000295317_ENST00000456652_TCGA-24-1553-01A_RNF149_chr2_101910476_-_MAP4K4_chr2_102407182_length(amino acids)=1268AA_start in transcript=75_stop in transcript=3881
MTPPSQLSGAAMAWRRREASVGARGVLALALLALALCVPGARGRALEWFSAVVNIEYVDPQTNLTVWSVSESGRFGDSSPKEGAHGLVGV
PWAPGGDLEGCAPDTRFFVPEPGGRGAAPWVALVARGGCTFKDKVLVAARRNASAVVLYNEERYGNITLPMSHAGTGNIVVIMISYPKGR
EILELVQKGIPVTMTIGVGTRHVQEFISGQSVVFVAIAFITMMIISLAWLIFYYIQRFLYTGSQIGSQSHRKETKKVIGQLLLHTVKHGE
KGRHVKTGQLAAIKVMDVTEDEEEEIKLEINMLKKYSHHRNIATYYGAFIKKSPPGHDDQLWLVMEFCGAGSITDLVKNTKGNTLKEDWI
AYISREILRGLAHLHIHHVIHRDIKGQNVLLTENAEVKLDETEYEYSGSEEEEEEVPEQEGEPSSIVNVPGESTLRRDFLRLQQENKERS
EALRRQQLLQEQQLREQEEYKRQLLAERQKRIEQQKEQRRRLEEQQRREREARRQQEREQRRREQEEKRRLEELERRRKEEEERRRAEEE
KRRVEREQEYIRRQLEEEQRHLEVLQQQLLQEQAMLLECRWREMEEHRQAERLQRQLQQEQAYLLSLQHDHRRPHPQHSQQPPPPQQERS
KPSFHAPEPKAHYEPADRAREVPVRTTSRSPVLSRRDSPLQGSGQQNSQAGQRNSTSIEPRLLWERVEKLVPRPGSGSSSGSSNSGSQPG
SHPGSQSGSGERFRVRSSSKSEGSPSQRLENAVKKPEDKKEVFRPLKPADLTALAKELRAVEDVRPPHKVTDYSSSSEESGTTDEEDDDV
EQEGADESTSGPEDTRAASSLNLSNGETESVKTMIVHDDVESEPAMTPSKEGTLIVRQTQSASSTLQKHKSSSSFTPFIDPRLLQISPSS
GTTVTSVVGFSCDGMRPEAIRQDPTRKGSVVNVNPTNTRPQSDTPEIRKYKKRFNSEILCAALWGVNLLVGTESGLMLLDRSGQGKVYPL
INRRRFQQMDVLEGLNVLVTISGKKDKLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDLEGCVHYKVVKYERIKFLVIALKSSVEVYAWA
PKPYHKFMAFKSFGELVHKPLLVDLTVEEGQRLKVIYGSCAGFHAVDVDSGSVYDIYLPTHIQCSIKPHAIIILPNTDGMELLVCYEDEG
VYVNTYGRITKDVVLQWGEMPTSVAYIRSNQTMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTL

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Fusion Gene PPI Analysis for RNF149-MAP4K4


check button Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in

ChiPPI page.


check button Protein-protein interactors with each fusion partner protein in wild-type (BIOGRID-3.4.160)
HgeneHgene's interactorsTgeneTgene's interactors


check button - Retained PPIs in in-frame fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenStill interaction with


check button - Lost PPIs in in-frame fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenInteraction lost with


check button - Retained PPIs, but lost function due to frame-shift fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenInteraction lost with


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Related Drugs for RNF149-MAP4K4


check button Drugs targeting genes involved in this fusion gene.
(DrugBank Version 5.1.8 2021-05-08)
PartnerGeneUniProtAccDrugBank IDDrug nameDrug activityDrug typeDrug status
TgeneMAP4K4O95819DB12010FostamatinibInhibitorSmall moleculeApproved|Investigational
TgeneMAP4K4O95819DB12010FostamatinibInhibitorSmall moleculeApproved|Investigational
TgeneMAP4K4O95819DB12010FostamatinibInhibitorSmall moleculeApproved|Investigational
TgeneMAP4K4O95819DB12010FostamatinibInhibitorSmall moleculeApproved|Investigational

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Related Diseases for RNF149-MAP4K4


check button Diseases associated with fusion partners.
(DisGeNet 4.0)
PartnerGeneDisease IDDisease name# pubmedsSource
TgeneMAP4K4C0024121Lung Neoplasms1CTD_human
TgeneMAP4K4C0242379Malignant neoplasm of lung1CTD_human