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Center for Computational Systems Medicine
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Fusion Gene Summary

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Fusion Gene ORF analysis

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Fusion Genomic Features

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Fusion Protein Features

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Fusion Gene Sequence

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Fusion Gene PPI analysis

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Related Drugs

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Related Diseases

Fusion gene:RPS6KA1-LIN28A (FusionGDB2 ID:77541)

Fusion Gene Summary for RPS6KA1-LIN28A

check button Fusion gene summary
Fusion gene informationFusion gene name: RPS6KA1-LIN28A
Fusion gene ID: 77541
HgeneTgene
Gene symbol

RPS6KA1

LIN28A

Gene ID

6195

79727

Gene nameribosomal protein S6 kinase A1lin-28 homolog A
SynonymsHU-1|MAPKAPK1|MAPKAPK1A|RSK|RSK1|p90RskCSDD1|LIN-28|LIN28|ZCCHC1|lin-28A
Cytomap

1p36.11

1p36.11

Type of geneprotein-codingprotein-coding
Descriptionribosomal protein S6 kinase alpha-190 kDa ribosomal protein S6 kinase 1MAP kinase-activated protein kinase 1aMAPK-activated protein kinase 1aMAPKAP kinase 1aMAPKAPK-1aRSK-1S6K-alpha 1dJ590P13.1 (ribosomal protein S6 kinase, 90kD, polypeptide 1)p9protein lin-28 homolog ARNA-binding protein LIN-28zinc finger CCHC domain-containing protein 1zinc finger, CCHC domain containing 1
Modification date2020032720200322
UniProtAcc.

Q9H9Z2

Ensembl transtripts involved in fusion geneENST00000374168, ENST00000374166, 
ENST00000526792, ENST00000374162, 
ENST00000530003, ENST00000531382, 
ENST00000488985, 		ENST00000326279, 
ENST00000254231, 
Fusion gene scores* DoF score3 X 4 X 3=362 X 2 X 2=8
# samples 52
** MAII scorelog2(5/36*10)=0.473931188332412
effective Gene in Pan-Cancer Fusion Genes (eGinPCFGs).
DoF>8 and MAII>0		log2(2/8*10)=1.32192809488736
Context

PubMed: RPS6KA1 [Title/Abstract] AND LIN28A [Title/Abstract] AND fusion [Title/Abstract]

Most frequent breakpointRPS6KA1(26885428)-LIN28A(26751794), # samples:3
Anticipated loss of major functional domain due to fusion event.
* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

check button Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez
PartnerGeneGO IDGO termPubMed ID
HgeneRPS6KA1

GO:0043154

negative regulation of cysteine-type endopeptidase activity involved in apoptotic process

18402937


check buttonFusion gene breakpoints across RPS6KA1 (5'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

check buttonFusion gene breakpoints across LIN28A (3'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

check button Fusion gene information from two resources (ChiTars 5.0 and ChimerDB 4.0)
* All genome coordinats were lifted-over on hg19.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.
SourceDiseaseSampleHgeneHchrHbpHstrandTgeneTchrTbpTstrand
ChimerDB4LUADTCGA-55-8085-01ARPS6KA1chr1

26885428

+LIN28Achr1

26751794

+
ChimerDB4LUADTCGA-55-8085-01ARPS6KA1chr1

26885428

+LIN28Achr1

26751794

+
ChimerDB4LUADTCGA-55-8085-01ARPS6KA1chr1

26885428

-LIN28Achr1

26751794

+


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Fusion Gene ORF analysis for RPS6KA1-LIN28A

check button Open reading frame (ORF) analsis of fusion genes based on Ensembl gene isoform structure.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.
ORFHenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrand
In-frameENST00000374168ENST00000326279RPS6KA1chr1

26885428

+LIN28Achr1

26751794

+
In-frameENST00000374168ENST00000254231RPS6KA1chr1

26885428

+LIN28Achr1

26751794

+
In-frameENST00000374166ENST00000326279RPS6KA1chr1

26885428

+LIN28Achr1

26751794

+
In-frameENST00000374166ENST00000254231RPS6KA1chr1

26885428

+LIN28Achr1

26751794

+
In-frameENST00000526792ENST00000326279RPS6KA1chr1

26885428

+LIN28Achr1

26751794

+
In-frameENST00000526792ENST00000254231RPS6KA1chr1

26885428

+LIN28Achr1

26751794

+
In-frameENST00000374162ENST00000326279RPS6KA1chr1

26885428

+LIN28Achr1

26751794

+
In-frameENST00000374162ENST00000254231RPS6KA1chr1

26885428

+LIN28Achr1

26751794

+
In-frameENST00000530003ENST00000326279RPS6KA1chr1

26885428

+LIN28Achr1

26751794

+
In-frameENST00000530003ENST00000254231RPS6KA1chr1

26885428

+LIN28Achr1

26751794

+
In-frameENST00000531382ENST00000326279RPS6KA1chr1

26885428

+LIN28Achr1

26751794

+
In-frameENST00000531382ENST00000254231RPS6KA1chr1

26885428

+LIN28Achr1

26751794

+
intron-3CDSENST00000488985ENST00000326279RPS6KA1chr1

26885428

+LIN28Achr1

26751794

+
intron-3CDSENST00000488985ENST00000254231RPS6KA1chr1

26885428

+LIN28Achr1

26751794

+

check buttonORFfinder result based on the fusion transcript sequence of in-frame fusion genes.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandSeq length
(transcript)		BP loci
(transcript)		Predicted start
(transcript)		Predicted stop
(transcript)		Seq length
(amino acids)
ENST00000374168RPS6KA1chr126885428+ENST00000326279LIN28Achr126751794+503513691541770538
ENST00000374168RPS6KA1chr126885428+ENST00000254231LIN28Achr126751794+452413691541770538
ENST00000374166RPS6KA1chr126885428+ENST00000326279LIN28Achr126751794+499113251431726527
ENST00000374166RPS6KA1chr126885428+ENST00000254231LIN28Achr126751794+448013251431726527
ENST00000526792RPS6KA1chr126885428+ENST00000326279LIN28Achr126751794+503913731011774557
ENST00000526792RPS6KA1chr126885428+ENST00000254231LIN28Achr126751794+452813731011774557
ENST00000374162RPS6KA1chr126885428+ENST00000326279LIN28Achr126751794+4812114601547515
ENST00000374162RPS6KA1chr126885428+ENST00000254231LIN28Achr126751794+4301114601547515
ENST00000530003RPS6KA1chr126885428+ENST00000326279LIN28Achr126751794+48831217261618530
ENST00000530003RPS6KA1chr126885428+ENST00000254231LIN28Achr126751794+43721217261618530
ENST00000531382RPS6KA1chr126885428+ENST00000326279LIN28Achr126751794+49571291131692559
ENST00000531382RPS6KA1chr126885428+ENST00000254231LIN28Achr126751794+44461291131692559

check buttonDeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandNo-coding scoreCoding score
ENST00000374168ENST00000326279RPS6KA1chr126885428+LIN28Achr126751794+0.0004222530.9995777
ENST00000374168ENST00000254231RPS6KA1chr126885428+LIN28Achr126751794+0.0003463960.9996536
ENST00000374166ENST00000326279RPS6KA1chr126885428+LIN28Achr126751794+0.0002409370.999759
ENST00000374166ENST00000254231RPS6KA1chr126885428+LIN28Achr126751794+0.0001957930.9998042
ENST00000526792ENST00000326279RPS6KA1chr126885428+LIN28Achr126751794+0.0018057920.9981943
ENST00000526792ENST00000254231RPS6KA1chr126885428+LIN28Achr126751794+0.0015583670.99844164
ENST00000374162ENST00000326279RPS6KA1chr126885428+LIN28Achr126751794+0.0016648540.9983352
ENST00000374162ENST00000254231RPS6KA1chr126885428+LIN28Achr126751794+0.0014901380.9985098
ENST00000530003ENST00000326279RPS6KA1chr126885428+LIN28Achr126751794+0.0006825660.9993174
ENST00000530003ENST00000254231RPS6KA1chr126885428+LIN28Achr126751794+0.0005536860.99944633
ENST00000531382ENST00000326279RPS6KA1chr126885428+LIN28Achr126751794+0.0008229040.99917704
ENST00000531382ENST00000254231RPS6KA1chr126885428+LIN28Achr126751794+0.0006418250.99935824

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Fusion Genomic Features for RPS6KA1-LIN28A


check buttonFusionAI prediction of the potential fusion gene breakpoint based on the pre-mature RNA sequence context (+/- 5kb of individual partner genes, total 20kb length sequence). FusionAI is a fusion gene breakpoint classifier based on convolutional neural network by comparing the fusion positive and negative sequence context of ~ 20K fusion gene data. From here, we can have the relative potentency of the 20K genomic sequence how individual sequnce will be likely used as the gene fusion breakpoints.
HgeneHchrHbpHstrandTgeneTchrTbpTstrand1-pp (fusion gene breakpoint)
RPS6KA1chr126885428+LIN28Achr126751793+9.98E-070.99999905
RPS6KA1chr126885428+LIN28Achr126751793+9.98E-070.99999905

check buttonDistribution of 44 human genomic features loci across 20kb length fusion breakpoint regions. We integrated a total of 44 different types of human genomic feature loci information across five big categories including virus integration sites, repeats, structural variants, chromatin states, and gene expression regulation. More details are in help page.

check buttonDistribution of 44 human genomic features loci across 20kb length fusion breakpoint regions that are ovelapped with the top 1% feature importance score regions. More details are in help page.

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Fusion Protein Features for RPS6KA1-LIN28A


check button Go to

FGviewer for the breakpoints of chr1:26885428-chr1:26751794

.
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
FGviewer

check buttonMain function of each fusion partner protein. (from UniProt)
HgeneTgene
.LIN28A

Q9H9Z2

FUNCTION: Might normally function as a transcriptional repressor. EWS-fusion-proteins (EFPS) may play a role in the tumorigenic process. They may disturb gene expression by mimicking, or interfering with the normal function of CTD-POLII within the transcription initiation complex. They may also contribute to an aberrant activation of the fusion protein target genes.FUNCTION: RNA-binding protein that inhibits processing of pre-let-7 miRNAs and regulates translation of mRNAs that control developmental timing, pluripotency and metabolism (PubMed:21247876). Seems to recognize a common structural G-quartet (G4) feature in its miRNA and mRNA targets (Probable). 'Translational enhancer' that drives specific mRNAs to polysomes and increases the efficiency of protein synthesis. Its association with the translational machinery and target mRNAs results in an increased number of initiation events per molecule of mRNA and, indirectly, in mRNA stabilization. Binds IGF2 mRNA, MYOD1 mRNA, ARBP/36B4 ribosomal protein mRNA and its own mRNA. Essential for skeletal muscle differentiation program through the translational up-regulation of IGF2 expression. Suppressor of microRNA (miRNA) biogenesis, including that of let-7, miR107, miR-143 and miR-200c. Specifically binds the miRNA precursors (pre-miRNAs), recognizing an 5'-GGAG-3' motif found in pre-miRNA terminal loop, and recruits TUT4 AND tut7 uridylyltransferaseS. This results in the terminal uridylation of target pre-miRNAs. Uridylated pre-miRNAs fail to be processed by Dicer and undergo degradation. The repression of let-7 expression is required for normal development and contributes to maintain the pluripotent state by preventing let-7-mediated differentiation of embryonic stem cells (PubMed:18951094, PubMed:19703396, PubMed:22118463, PubMed:22898984). Localized to the periendoplasmic reticulum area, binds to a large number of spliced mRNAs and inhibits the translation of mRNAs destined for the ER, reducing the synthesis of transmembrane proteins, ER or Golgi lumen proteins, and secretory proteins. Binds to and enhances the translation of mRNAs for several metabolic enzymes, such as PFKP, PDHA1 or SDHA, increasing glycolysis and oxidative phosphorylation. Which, with the let-7 repression may enhance tissue repair in adult tissue (By similarity). {ECO:0000250|UniProtKB:Q8K3Y3, ECO:0000269|PubMed:18951094, ECO:0000269|PubMed:19703396, ECO:0000269|PubMed:21247876, ECO:0000269|PubMed:22118463, ECO:0000269|PubMed:22898984, ECO:0000305}.

check buttonRetention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at

download page


* Minus value of BPloci means that the break pointn is located before the CDS.
- In-frame and retained protein feature among the 13 regional features.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note
HgeneRPS6KA1chr1:26885428chr1:26751794ENST00000374168+1422322_391405.0736.0DomainAGC-kinase C-terminal
HgeneRPS6KA1chr1:26885428chr1:26751794ENST00000374168+142262_321405.0736.0DomainProtein kinase 1
HgeneRPS6KA1chr1:26885428chr1:26751794ENST00000531382+1321322_391414.0745.0DomainAGC-kinase C-terminal
HgeneRPS6KA1chr1:26885428chr1:26751794ENST00000531382+132162_321414.0745.0DomainProtein kinase 1
HgeneRPS6KA1chr1:26885428chr1:26751794ENST00000374162+132168_76313.0644.0Nucleotide bindingATP
HgeneRPS6KA1chr1:26885428chr1:26751794ENST00000374168+142268_76405.0736.0Nucleotide bindingATP
HgeneRPS6KA1chr1:26885428chr1:26751794ENST00000526792+142268_76313.0644.0Nucleotide bindingATP
HgeneRPS6KA1chr1:26885428chr1:26751794ENST00000531382+132168_76414.0745.0Nucleotide bindingATP
TgeneLIN28Achr1:26885428chr1:26751794ENST0000025423115113_13676.0382.6666666666667RegionNote=Flexible linker
TgeneLIN28Achr1:26885428chr1:26751794ENST0000032627914113_13676.0210.0RegionNote=Flexible linker
TgeneLIN28Achr1:26885428chr1:26751794ENST0000025423115137_15476.0382.6666666666667Zinc fingerCCHC-type 1
TgeneLIN28Achr1:26885428chr1:26751794ENST0000025423115159_17676.0382.6666666666667Zinc fingerCCHC-type 2
TgeneLIN28Achr1:26885428chr1:26751794ENST0000032627914137_15476.0210.0Zinc fingerCCHC-type 1
TgeneLIN28Achr1:26885428chr1:26751794ENST0000032627914159_17676.0210.0Zinc fingerCCHC-type 2

- In-frame and not-retained protein feature among the 13 regional features.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note
HgeneRPS6KA1chr1:26885428chr1:26751794ENST00000374162+1321322_391313.0644.0DomainAGC-kinase C-terminal
HgeneRPS6KA1chr1:26885428chr1:26751794ENST00000374162+1321418_675313.0644.0DomainProtein kinase 2
HgeneRPS6KA1chr1:26885428chr1:26751794ENST00000374162+132162_321313.0644.0DomainProtein kinase 1
HgeneRPS6KA1chr1:26885428chr1:26751794ENST00000374168+1422418_675405.0736.0DomainProtein kinase 2
HgeneRPS6KA1chr1:26885428chr1:26751794ENST00000526792+1422322_391313.0644.0DomainAGC-kinase C-terminal
HgeneRPS6KA1chr1:26885428chr1:26751794ENST00000526792+1422418_675313.0644.0DomainProtein kinase 2
HgeneRPS6KA1chr1:26885428chr1:26751794ENST00000526792+142262_321313.0644.0DomainProtein kinase 1
HgeneRPS6KA1chr1:26885428chr1:26751794ENST00000531382+1321418_675414.0745.0DomainProtein kinase 2
HgeneRPS6KA1chr1:26885428chr1:26751794ENST00000374162+1321424_432313.0644.0Nucleotide bindingATP
HgeneRPS6KA1chr1:26885428chr1:26751794ENST00000374168+1422424_432405.0736.0Nucleotide bindingATP
HgeneRPS6KA1chr1:26885428chr1:26751794ENST00000526792+1422424_432313.0644.0Nucleotide bindingATP
HgeneRPS6KA1chr1:26885428chr1:26751794ENST00000531382+1321424_432414.0745.0Nucleotide bindingATP
TgeneLIN28Achr1:26885428chr1:26751794ENST000002542311539_11276.0382.6666666666667DomainNote=CSD
TgeneLIN28Achr1:26885428chr1:26751794ENST000003262791439_11276.0210.0DomainNote=CSD


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Fusion Gene Sequence for RPS6KA1-LIN28A


check button For in-frame fusion transcripts, we provide the fusion transcript sequences and fusion amino acid sequences. To have fusion amino acid sequence, we ran ORFfinder and chose the longest ORF among the all predicted ones.
>In-frame_ENST00000374168_ENST00000326279_TCGA-55-8085-01A_RPS6KA1_chr1_26885428_+_LIN28A_chr1_26751794_length(transcript)=5035nt_BP=1369nt
CTAGTGCCGCGGCGGCGGCGGCGGACGGCCCAGCCGGAGCGCGAGGGGCTCGGGGGGGCGCGGCGGTTCGGGTCGCAGAGCCAGGGACCC
CAGGACCCGGGAGGCGGCGCAGCCGGGGCCGCCGGAGGAGCGCGGGTGACCTGGCGGCGGCGAGATGCCGCTCGCCCAGCTCAAGGAGCC
CTGGCCGCTCATGGAGCTAGTGCCTCTGGACCCGGAGAATGGACAGACCTCAGGGGAAGAAGCTGGACTTCAGCCGTCCAAGGATGAGGG
CGTCCTCAAGGAGATCTCCATCACGCACCACGTCAAGGCTGGCTCTGAGAAGGCTGATCCATCCCATTTCGAGCTCCTCAAGGTTCTGGG
CCAGGGATCCTTTGGCAAAGTCTTCCTGGTGCGGAAAGTCACCCGGCCTGACAGTGGGCACCTGTATGCTATGAAGGTGCTGAAGAAGGC
AACGCTGAAAGTACGTGACCGCGTCCGGACCAAGATGGAGAGAGACATCCTGGCTGATGTAAATCACCCATTCGTGGTGAAGCTGCACTA
TGCCTTCCAGACCGAGGGCAAGCTCTATCTCATTCTGGACTTCCTGCGTGGTGGGGACCTCTTCACCCGGCTCTCAAAAGAGGTGATGTT
CACGGAGGAGGATGTGAAGTTTTACCTGGCTGAGCTGGCTCTGGGCCTGGATCACCTGCACAGCCTGGGTATCATTTACAGAGACCTCAA
GCCTGAGAACATCCTTCTGGATGAGGAGGGCCACATCAAACTCACTGACTTTGGCCTGAGCAAAGAGGCCATTGACCACGAGAAGAAGGC
CTATTCTTTCTGCGGGACAGTGGAGTACATGGCCCCTGAGGTCGTCAACCGCCAGGGCCACTCCCATAGTGCGGACTGGTGGTCCTATGG
GGTGTTGATGTTTGAGATGCTGACGGGCTCCCTGCCCTTCCAGGGGAAGGACCGGAAGGAGACCATGACACTGATTCTGAAGGCGAAGCT
AGGCATGCCCCAGTTTCTGAGCACTGAAGCCCAGAGCCTCTTGCGGGCCCTGTTCAAGCGGAATCCTGCCAACCGGCTCGGCTCCGGCCC
TGATGGGGCAGAGGAAATCAAGCGGCATGTCTTCTACTCCACCATTGACTGGAATAAGCTATACCGTCGTGAGATCAAGCCACCCTTCAA
GCCAGCAGTGGCTCAGCCTGATGACACCTTCTACTTTGACACCGAGTTCACGTCCCGCACACCCAAGGATTCCCCAGGCATCCCCCCCAG
CGCTGGGGCCCATCAGCTGTTCCGGGGCTTCAGCTTCGTGGCCACCGGCCTGATGGAAGACGACGGCAAGCCTCGTGCCCCGCAGGCACC
CCTGCACTCGGTGGTACAGAGTAAGCTGCACATGGAAGGGTTCCGGAGCTTGAAGGAGGGTGAGGCAGTGGAGTTCACCTTTAAGAAGTC
AGCCAAGGGTCTGGAATCCATCCGTGTCACCGGACCTGGTGGAGTATTCTGTATTGGGAGTGAGAGGCGGCCAAAAGGAAAGAGCATGCA
GAAGCGCAGATCAAAAGGAGACAGGTGCTACAACTGTGGAGGTCTAGATCATCATGCCAAGGAATGCAAGCTGCCACCCCAGCCCAAGAA
GTGCCACTTCTGCCAGAGCATCAGCCATATGGTAGCCTCATGTCCGCTGAAGGCCCAGCAGGGCCCTAGTGCACAGGGAAAGCCAACCTA
CTTTCGAGAGGAAGAAGAAGAAATCCACAGCCCTACCCTGCTCCCGGAGGCACAGAATTGAGCCACAATGGGTGGGGGCTATTCTTTTGC
TATCAGGAAGTTTTGAGGAGCAGGCAGAGTGGAGAAAGTGGGAATAGGGTGCATTGGGGCTAGTTGGCACTGCCATGTATCTCAGGCTTG
GGTTCACACCATCACCCTTTCTTCCCTCTAGGTGGGGGGAAAGGGTGAGTCAAAGGAACTCCAACCATGCTCTGTCCAAATGCAAGTGAG
GGTTCTGGGGGCAACCAGGAGGGGGGAATCACCCTACAACCTGCATACTTTGAGTCTCCATCCCCAGAATTTCCAGCTTTTGAAAGTGGC
CTGGATAGGGAAGTTGTTTTCCTTTTAAAGAAGGATATATAATAATTCCCATGCCAGAGTGAAATGATTAAGTATAAGACCAGATTCATG
GAGCCAAGCCACTACATTCTGTGGAAGGAGATCTCTCAGGAGTAAGCATTGTTTTTTTTTCACATCTTGTATCCTCATACCCACTTTTGG
GATAGGGTGCTGGCAGCTGTCCCAAGCAATGGGTAATGATGATGGCAAAAAGGGTGTTTGGGGGAACAGCTGCAGACCTGCTGCTCTATG
CTCACCCCCGCCCCATTCTGGGCCAATGTGATTTTATTTATTTGCTCCCTTGGATACTGCACCTTGGGTCCCACTTTCTCCAGGATGCCA
ACTGCACTAGCTGTGTGCGAATGACGTATCTTGTGCATTTTAACTTTTTTTCCTTAATATAAATATTCTGGTTTTGTATTTTTGTATATT
TTAATCTAAGGCCCTCATTTCCTGCACTGTGTTCTCAGGTACATGAGCAATCTCAGGGATAGCCAGCAGCAGCTCCAGGTCTGCGCAGCA
GGAATTACTTTTTGTTGTTTTTGCCACCGTGGAGAGCAACTATTTGGAGTGCACAGCCTATTGAACTACCTCATTTTTGCCAATAAGAGC
TGGCTTTTCTGCCATAGTGTCCTCTTGAAACCCCCTCTGCCTTGAAAATGTTTTATGGGAGACTAGGTTTTAACTGGGTGGCCCCATGAC
TTGATTGCCTTCTACTGGAAGATTGGGAATTAGTCTAAACAGGAAATGGTGGTACACAGAGGCTAGGAGAGGCTGGGCCCGGTGAAAAGG
CCAGAGAGCAAGCCAAGATTAGGTGAGGGTTGTCTAATCCTATGGCACAGGACGTGCTTTACATCTCCAGATCTGTTCTTCACCAGATTA
GGTTAGGCCTACCATGTGCCACAGGGTGTGTGTGTGTTTGTAAAACTAGAGTTGCTAAGGATAAGTTTAAAGACCAATACCCCTGTACTT
AATCCTGTGCTGTCGAGGGATGGATATATGAAGTAAGGTGAGATCCTTAACCTTTCAAAATTTTCGGGTTCCAGGGAGACACACAAGCGA
GGGTTTTGTGGTGCCTGGAGCCTGTGTCCTGCCCTGCTACAGTAGTGATTAATAGTGTCATGGTAGCTAAAGGAGAAAAAGGGGGTTTCG
TTTACACGCTGTGAGATCACCGCAAACCTACCTTACTGTGTTGAAACGGGACAAATGCAATAGAACGCATTGGGTGGTGTGTGTCTGATC
CTGGGTTCTTGTCTCCCCTAAATGCTGCCCCCCAAGTTACTGTATTTGTCTGGGCTTTGTAGGACTTCACTACGTTGATTGCTAGGTGGC
CTAGTTTGTGTAAATATAATGTATTGGTCTTTCTCCGTGTTCTTTGGGGGTTTTGTTTACAAACTTCTTTTTGTATTGAGAGAAAAATAG
CCAAAGCATCTTTGACAGAAGGTTCTGCACCAGGCAAAAAGATCTGAAACATTAGTTTGGGGGGCCCTCTTCTTAAAGTGGGGATCTTGA
ACCATCCTTTCTTTTGTATTCCCCTTCCCCTATTACCTATTAGACCAGATCTTCTGTCCTAAAAACTTGTCTTCTACCCTGCCCTCTTTT
CTGTTCACCCCCAAAAGAAAACTTACACACCCACACACATACACATTTCATGCTTGGAGTGTCTCCACAACTCTTAAATGATGTATGCAA
AAATACTGAAGCTAGGAAAACCCTCCATCCCTTGTTCCCAACCTCCTAAGTCAAGACCATTACCATTTCTTTCTTTCTTTTTTTTTTTTT
TTTAAAATGGAGTCTCACTGTGTCACCCAGGCTGGAGTGCAGTGGCATGATCGGCTCACTGCAGCCTCTGCCTCTTGGGTTCAAGTGATT
CTCCTGCCTCAGCCTCCTGAGTAGCTGGGATTTCAGGCACCCGCCACACTCAGCTAATTTTTGTATTTTTAGTAGAGACGGGGTTTCACC
ATGTTGTCCAGGCTGGTCTGGAACTCCTGACCTCAGGTGATCTGCCCACCTTGGCTTCCCAAAGTGCTGGGATTACAGGCATGAGCCACC
ATGCTGGGCCAACCATTTCTTGGTGTATTCATGCCAAACACTTAAGACACTGCTGTAGCCCAGGCGCGGTGGCTCACACCTGTAATCCCA
GCACTTTGGAAGGCTGAGGCGGGCGGATCACAAGGTCACGAGTTCAAAACTATCCTGGCCAACACAGTGAAACCCCGTCTCTACTAAAAT
ACAAAAAAATTAGCCGGGTGTGGTGGTGCATGCCTTTAGTCCTAGCTATTCAGGAGGCTGAGGCAGGGGAATCGCTTGAACCCGAGAGGC
AGAGGTTGCAGTGAGCTGAGATCGCACCACTGCACTCCAGCCTGGTTACAGAGCAAGACTCTGTCTCAAACAAAACAAAACAAAACAAAA
ACACACTACTGTATTTTGGATGGATCAAACCTCCTTAATTTTAATTTCTAATCCTAAAGTAAAGAGATGCAATTGGGGGCCTTCCATGTA
GAAAGTGGGGTCAGGAGGCCAAGAAAGGGAATATGAATGTATATCCAAGTCACTCAGGAACTTTTATGCAGGTGCTAGAAACTTTATGTC
AAAGTGGCCACAAGATTGTTTAATAGGAGACGAACGAATGTAACTCCATGTTTACTGCTAAAAACCAAAGCTTTGTGTAAAATCTTGAAT
TTATGGGGCGGGAGGGTAGGAAAGCCTGTACCTGTCTGTTTTTTTCCTGATCCTTTTCCCTCATTCCTGAACTGCAGGAGACTGAGCCCC
TTTGGGCTTTGGTGACCCCATCACTGGGGTGTGTTTATTTGATGGTTGATTTTGCTGTACTGGGTACTTCCTTTCCCATTTTCTAATCAT

>In-frame_ENST00000374168_ENST00000326279_TCGA-55-8085-01A_RPS6KA1_chr1_26885428_+_LIN28A_chr1_26751794_length(amino acids)=538AA_start in transcript=154_stop in transcript=1770
MPLAQLKEPWPLMELVPLDPENGQTSGEEAGLQPSKDEGVLKEISITHHVKAGSEKADPSHFELLKVLGQGSFGKVFLVRKVTRPDSGHL
YAMKVLKKATLKVRDRVRTKMERDILADVNHPFVVKLHYAFQTEGKLYLILDFLRGGDLFTRLSKEVMFTEEDVKFYLAELALGLDHLHS
LGIIYRDLKPENILLDEEGHIKLTDFGLSKEAIDHEKKAYSFCGTVEYMAPEVVNRQGHSHSADWWSYGVLMFEMLTGSLPFQGKDRKET
MTLILKAKLGMPQFLSTEAQSLLRALFKRNPANRLGSGPDGAEEIKRHVFYSTIDWNKLYRREIKPPFKPAVAQPDDTFYFDTEFTSRTP
KDSPGIPPSAGAHQLFRGFSFVATGLMEDDGKPRAPQAPLHSVVQSKLHMEGFRSLKEGEAVEFTFKKSAKGLESIRVTGPGGVFCIGSE

--------------------------------------------------------------
>In-frame_ENST00000374168_ENST00000254231_TCGA-55-8085-01A_RPS6KA1_chr1_26885428_+_LIN28A_chr1_26751794_length(transcript)=4524nt_BP=1369nt
CTAGTGCCGCGGCGGCGGCGGCGGACGGCCCAGCCGGAGCGCGAGGGGCTCGGGGGGGCGCGGCGGTTCGGGTCGCAGAGCCAGGGACCC
CAGGACCCGGGAGGCGGCGCAGCCGGGGCCGCCGGAGGAGCGCGGGTGACCTGGCGGCGGCGAGATGCCGCTCGCCCAGCTCAAGGAGCC
CTGGCCGCTCATGGAGCTAGTGCCTCTGGACCCGGAGAATGGACAGACCTCAGGGGAAGAAGCTGGACTTCAGCCGTCCAAGGATGAGGG
CGTCCTCAAGGAGATCTCCATCACGCACCACGTCAAGGCTGGCTCTGAGAAGGCTGATCCATCCCATTTCGAGCTCCTCAAGGTTCTGGG
CCAGGGATCCTTTGGCAAAGTCTTCCTGGTGCGGAAAGTCACCCGGCCTGACAGTGGGCACCTGTATGCTATGAAGGTGCTGAAGAAGGC
AACGCTGAAAGTACGTGACCGCGTCCGGACCAAGATGGAGAGAGACATCCTGGCTGATGTAAATCACCCATTCGTGGTGAAGCTGCACTA
TGCCTTCCAGACCGAGGGCAAGCTCTATCTCATTCTGGACTTCCTGCGTGGTGGGGACCTCTTCACCCGGCTCTCAAAAGAGGTGATGTT
CACGGAGGAGGATGTGAAGTTTTACCTGGCTGAGCTGGCTCTGGGCCTGGATCACCTGCACAGCCTGGGTATCATTTACAGAGACCTCAA
GCCTGAGAACATCCTTCTGGATGAGGAGGGCCACATCAAACTCACTGACTTTGGCCTGAGCAAAGAGGCCATTGACCACGAGAAGAAGGC
CTATTCTTTCTGCGGGACAGTGGAGTACATGGCCCCTGAGGTCGTCAACCGCCAGGGCCACTCCCATAGTGCGGACTGGTGGTCCTATGG
GGTGTTGATGTTTGAGATGCTGACGGGCTCCCTGCCCTTCCAGGGGAAGGACCGGAAGGAGACCATGACACTGATTCTGAAGGCGAAGCT
AGGCATGCCCCAGTTTCTGAGCACTGAAGCCCAGAGCCTCTTGCGGGCCCTGTTCAAGCGGAATCCTGCCAACCGGCTCGGCTCCGGCCC
TGATGGGGCAGAGGAAATCAAGCGGCATGTCTTCTACTCCACCATTGACTGGAATAAGCTATACCGTCGTGAGATCAAGCCACCCTTCAA
GCCAGCAGTGGCTCAGCCTGATGACACCTTCTACTTTGACACCGAGTTCACGTCCCGCACACCCAAGGATTCCCCAGGCATCCCCCCCAG
CGCTGGGGCCCATCAGCTGTTCCGGGGCTTCAGCTTCGTGGCCACCGGCCTGATGGAAGACGACGGCAAGCCTCGTGCCCCGCAGGCACC
CCTGCACTCGGTGGTACAGAGTAAGCTGCACATGGAAGGGTTCCGGAGCTTGAAGGAGGGTGAGGCAGTGGAGTTCACCTTTAAGAAGTC
AGCCAAGGGTCTGGAATCCATCCGTGTCACCGGACCTGGTGGAGTATTCTGTATTGGGAGTGAGAGGCGGCCAAAAGGAAAGAGCATGCA
GAAGCGCAGATCAAAAGGAGACAGGTGCTACAACTGTGGAGGTCTAGATCATCATGCCAAGGAATGCAAGCTGCCACCCCAGCCCAAGAA
GTGCCACTTCTGCCAGAGCATCAGCCATATGGTAGCCTCATGTCCGCTGAAGGCCCAGCAGGGCCCTAGTGCACAGGGAAAGCCAACCTA
CTTTCGAGAGGAAGAAGAAGAAATCCACAGCCCTACCCTGCTCCCGGAGGCACAGAATTGAGCCACAATGGGTGGGGGCTATTCTTTTGC
TATCAGGAAGTTTTGAGGAGCAGGCAGAGTGGAGAAAGTGGGAATAGGGTGCATTGGGGCTAGTTGGCACTGCCATGTATCTCAGGCTTG
GGTTCACACCATCACCCTTTCTTCCCTCTAGGTGGGGGGAAAGGGTGAGTCAAAGGAACTCCAACCATGCTCTGTCCAAATGCAAGTGAG
GGTTCTGGGGGCAACCAGGAGGGGGGAATCACCCTACAACCTGCATACTTTGAGTCTCCATCCCCAGAATTTCCAGCTTTTGAAAGTGGC
CTGGATAGGGAAGTTGTTTTCCTTTTAAAGAAGGATATATAATAATTCCCATGCCAGAGTGAAATGATTAAGTATAAGACCAGATTCATG
GAGCCAAGCCACTACATTCTGTGGAAGGAGATCTCTCAGGAGTAAGCATTGTTTTTTTTTCACATCTTGTATCCTCATACCCACTTTTGG
GATAGGGTGCTGGCAGCTGTCCCAAGCAATGGGTAATGATGATGGCAAAAAGGGTGTTTGGGGGAACAGCTGCAGACCTGCTGCTCTATG
CTCACCCCCGCCCCATTCTGGGCCAATGTGATTTTATTTATTTGCTCCCTTGGATACTGCACCTTGGGTCCCACTTTCTCCAGGATGCCA
ACTGCACTAGCTGTGTGCGAATGACGTATCTTGTGCATTTTAACTTTTTTTCCTTAATATAAATATTCTGGTTTTGTATTTTTGTATATT
TTAATCTAAGGCCCTCATTTCCTGCACTGTGTTCTCAGGTACATGAGCAATCTCAGGGATAGCCAGCAGCAGCTCCAGGTCTGCGCAGCA
GGAATTACTTTTTGTTGTTTTTGCCACCGTGGAGAGCAACTATTTGGAGTGCACAGCCTATTGAACTACCTCATTTTTGCCAATAAGAGC
TGGCTTTTCTGCCATAGTGTCCTCTTGAAACCCCCTCTGCCTTGAAAATGTTTTATGGGAGACTAGGTTTTAACTGGGTGGCCCCATGAC
TTGATTGCCTTCTACTGGAAGATTGGGAATTAGTCTAAACAGGAAATGGTGGTACACAGAGGCTAGGAGAGGCTGGGCCCGGTGAAAAGG
CCAGAGAGCAAGCCAAGATTAGGTGAGGGTTGTCTAATCCTATGGCACAGGACGTGCTTTACATCTCCAGATCTGTTCTTCACCAGATTA
GGTTAGGCCTACCATGTGCCACAGGGTGTGTGTGTGTTTGTAAAACTAGAGTTGCTAAGGATAAGTTTAAAGACCAATACCCCTGTACTT
AATCCTGTGCTGTCGAGGGATGGATATATGAAGTAAGGTGAGATCCTTAACCTTTCAAAATTTTCGGGTTCCAGGGAGACACACAAGCGA
GGGTTTTGTGGTGCCTGGAGCCTGTGTCCTGCCCTGCTACAGTAGTGATTAATAGTGTCATGGTAGCTAAAGGAGAAAAAGGGGGTTTCG
TTTACACGCTGTGAGATCACCGCAAACCTACCTTACTGTGTTGAAACGGGACAAATGCAATAGAACGCATTGGGTGGTGTGTGTCTGATC
CTGGGTTCTTGTCTCCCCTAAATGCTGCCCCCCAAGTTACTGTATTTGTCTGGGCTTTGTAGGACTTCACTACGTTGATTGCTAGGTGGC
CTAGTTTGTGTAAATATAATGTATTGGTCTTTCTCCGTGTTCTTTGGGGGTTTTGTTTACAAACTTCTTTTTGTATTGAGAGAAAAATAG
CCAAAGCATCTTTGACAGAAGGTTCTGCACCAGGCAAAAAGATCTGAAACATTAGTTTGGGGGGCCCTCTTCTTAAAGTGGGGATCTTGA
ACCATCCTTTCTTTTGTATTCCCCTTCCCCTATTACCTATTAGACCAGATCTTCTGTCCTAAAAACTTGTCTTCTACCCTGCCCTCTTTT
CTGTTCACCCCCAAAAGAAAACTTACACACCCACACACATACACATTTCATGCTTGGAGTGTCTCCACAACTCTTAAATGATGTATGCAA
AAATACTGAAGCTAGGAAAACCCTCCATCCCTTGTTCCCAACCTCCTAAGTCAAGACCATTACCATTTCTTTCTTTCTTTTTTTTTTTTT
TTTAAAATGGAGTCTCACTGTGTCACCCAGGCAGAGGTTGCAGTGAGCTGAGATCGCACCACTGCACTCCAGCCTGGTTACAGAGCAAGA
CTCTGTCTCAAACAAAACAAAACAAAACAAAAACACACTACTGTATTTTGGATGGATCAAACCTCCTTAATTTTAATTTCTAATCCTAAA
GTAAAGAGATGCAATTGGGGGCCTTCCATGTAGAAAGTGGGGTCAGGAGGCCAAGAAAGGGAATATGAATGTATATCCAAGTCACTCAGG
AACTTTTATGCAGGTGCTAGAAACTTTATGTCAAAGTGGCCACAAGATTGTTTAATAGGAGACGAACGAATGTAACTCCATGTTTACTGC
TAAAAACCAAAGCTTTGTGTAAAATCTTGAATTTATGGGGCGGGAGGGTAGGAAAGCCTGTACCTGTCTGTTTTTTTCCTGATCCTTTTC
CCTCATTCCTGAACTGCAGGAGACTGAGCCCCTTTGGGCTTTGGTGACCCCATCACTGGGGTGTGTTTATTTGATGGTTGATTTTGCTGT
ACTGGGTACTTCCTTTCCCATTTTCTAATCATTTTTTAACACAAGCTGACTCTTCCCTTCCCTTCTCCTTTCCCTGGGAAAATACAATGA

>In-frame_ENST00000374168_ENST00000254231_TCGA-55-8085-01A_RPS6KA1_chr1_26885428_+_LIN28A_chr1_26751794_length(amino acids)=538AA_start in transcript=154_stop in transcript=1770
MPLAQLKEPWPLMELVPLDPENGQTSGEEAGLQPSKDEGVLKEISITHHVKAGSEKADPSHFELLKVLGQGSFGKVFLVRKVTRPDSGHL
YAMKVLKKATLKVRDRVRTKMERDILADVNHPFVVKLHYAFQTEGKLYLILDFLRGGDLFTRLSKEVMFTEEDVKFYLAELALGLDHLHS
LGIIYRDLKPENILLDEEGHIKLTDFGLSKEAIDHEKKAYSFCGTVEYMAPEVVNRQGHSHSADWWSYGVLMFEMLTGSLPFQGKDRKET
MTLILKAKLGMPQFLSTEAQSLLRALFKRNPANRLGSGPDGAEEIKRHVFYSTIDWNKLYRREIKPPFKPAVAQPDDTFYFDTEFTSRTP
KDSPGIPPSAGAHQLFRGFSFVATGLMEDDGKPRAPQAPLHSVVQSKLHMEGFRSLKEGEAVEFTFKKSAKGLESIRVTGPGGVFCIGSE

--------------------------------------------------------------
>In-frame_ENST00000374166_ENST00000326279_TCGA-55-8085-01A_RPS6KA1_chr1_26885428_+_LIN28A_chr1_26751794_length(transcript)=4991nt_BP=1325nt
GCGGCGGCGGCGGACGGCCCAGCCGGAGCGCGAGGGGCTCGGGGGGGCGCGGCGGTTCGGGTCGCAGAGCCAGGGACCCCAGGACCCGGG
AGGCGGCGCAGCCGGGGCCGCCGGAGGAGCGCGGGTGACCTGGCGGCGGCGAGATGCCGCTCGCCCAGCTCAAGGAGCCCTGGCCGCTCA
TGGAGCTAGTGCCTCTGGACCCGGAGAATGGACAGACCTCAGGGGAAGAAGCTGGACTTCAGCCGTCCAAGGATGAGGGCGTCCTCAAGG
AGATCTCCATCACGCACCACGTCAAGGCTGGCTCTGAGAAGGCTGATCCATCCCATTTCGAGCTCCTCAAGGTTCTGGGCCAGGGATCCT
TTGGCAAAGTCTTCCTGGTGCGGAAAGTCACCCGGCCTGACAGTGGGCACCTGTATGCTATGAAGGTGCTGAAGAAGGCAACGCTGAAAG
TACGTGACCGCGTCCGGACCAAGATGGAGAGAGACATCCTGGCTGATGTAAATCACCCATTCGTGGTGAAGCTGCACTATGCCTTCCAGA
CCGAGGGCAAGCTCTATCTCATTCTGGACTTCCTGCGTGGTGGGGACCTCTTCACCCGGCTCTCAAAAGAGGTGATGTTCACGGAGGAGG
ATGTGAAGTTTTACCTGGCTGAGCTGGCTCTGGGCCTGGATCACCTGCACAGCCTGGGTATCATTTACAGAGACCTCAAGCCTGAGAACA
TCCTTCTGGATGAGGAGGGCCACATCAAACTCACTGACTTTGGCCTGAGCAAAGAGGCCATTGACCACGAGAAGAAGGCCTATTCTTTCT
GCGGGACAGTGGAGTACATGGCCCCTGAGGTCGTCAACCGCCAGGGCCACTCCCATAGTGCGGACTGGTGGTCCTATGGGGTGTTGATGG
GGAAGGACCGGAAGGAGACCATGACACTGATTCTGAAGGCGAAGCTAGGCATGCCCCAGTTTCTGAGCACTGAAGCCCAGAGCCTCTTGC
GGGCCCTGTTCAAGCGGAATCCTGCCAACCGGCTCGGCTCCGGCCCTGATGGGGCAGAGGAAATCAAGCGGCATGTCTTCTACTCCACCA
TTGACTGGAATAAGCTATACCGTCGTGAGATCAAGCCACCCTTCAAGCCAGCAGTGGCTCAGCCTGATGACACCTTCTACTTTGACACCG
AGTTCACGTCCCGCACACCCAAGGATTCCCCAGGCATCCCCCCCAGCGCTGGGGCCCATCAGCTGTTCCGGGGCTTCAGCTTCGTGGCCA
CCGGCCTGATGGAAGACGACGGCAAGCCTCGTGCCCCGCAGGCACCCCTGCACTCGGTGGTACAGAGTAAGCTGCACATGGAAGGGTTCC
GGAGCTTGAAGGAGGGTGAGGCAGTGGAGTTCACCTTTAAGAAGTCAGCCAAGGGTCTGGAATCCATCCGTGTCACCGGACCTGGTGGAG
TATTCTGTATTGGGAGTGAGAGGCGGCCAAAAGGAAAGAGCATGCAGAAGCGCAGATCAAAAGGAGACAGGTGCTACAACTGTGGAGGTC
TAGATCATCATGCCAAGGAATGCAAGCTGCCACCCCAGCCCAAGAAGTGCCACTTCTGCCAGAGCATCAGCCATATGGTAGCCTCATGTC
CGCTGAAGGCCCAGCAGGGCCCTAGTGCACAGGGAAAGCCAACCTACTTTCGAGAGGAAGAAGAAGAAATCCACAGCCCTACCCTGCTCC
CGGAGGCACAGAATTGAGCCACAATGGGTGGGGGCTATTCTTTTGCTATCAGGAAGTTTTGAGGAGCAGGCAGAGTGGAGAAAGTGGGAA
TAGGGTGCATTGGGGCTAGTTGGCACTGCCATGTATCTCAGGCTTGGGTTCACACCATCACCCTTTCTTCCCTCTAGGTGGGGGGAAAGG
GTGAGTCAAAGGAACTCCAACCATGCTCTGTCCAAATGCAAGTGAGGGTTCTGGGGGCAACCAGGAGGGGGGAATCACCCTACAACCTGC
ATACTTTGAGTCTCCATCCCCAGAATTTCCAGCTTTTGAAAGTGGCCTGGATAGGGAAGTTGTTTTCCTTTTAAAGAAGGATATATAATA
ATTCCCATGCCAGAGTGAAATGATTAAGTATAAGACCAGATTCATGGAGCCAAGCCACTACATTCTGTGGAAGGAGATCTCTCAGGAGTA
AGCATTGTTTTTTTTTCACATCTTGTATCCTCATACCCACTTTTGGGATAGGGTGCTGGCAGCTGTCCCAAGCAATGGGTAATGATGATG
GCAAAAAGGGTGTTTGGGGGAACAGCTGCAGACCTGCTGCTCTATGCTCACCCCCGCCCCATTCTGGGCCAATGTGATTTTATTTATTTG
CTCCCTTGGATACTGCACCTTGGGTCCCACTTTCTCCAGGATGCCAACTGCACTAGCTGTGTGCGAATGACGTATCTTGTGCATTTTAAC
TTTTTTTCCTTAATATAAATATTCTGGTTTTGTATTTTTGTATATTTTAATCTAAGGCCCTCATTTCCTGCACTGTGTTCTCAGGTACAT
GAGCAATCTCAGGGATAGCCAGCAGCAGCTCCAGGTCTGCGCAGCAGGAATTACTTTTTGTTGTTTTTGCCACCGTGGAGAGCAACTATT
TGGAGTGCACAGCCTATTGAACTACCTCATTTTTGCCAATAAGAGCTGGCTTTTCTGCCATAGTGTCCTCTTGAAACCCCCTCTGCCTTG
AAAATGTTTTATGGGAGACTAGGTTTTAACTGGGTGGCCCCATGACTTGATTGCCTTCTACTGGAAGATTGGGAATTAGTCTAAACAGGA
AATGGTGGTACACAGAGGCTAGGAGAGGCTGGGCCCGGTGAAAAGGCCAGAGAGCAAGCCAAGATTAGGTGAGGGTTGTCTAATCCTATG
GCACAGGACGTGCTTTACATCTCCAGATCTGTTCTTCACCAGATTAGGTTAGGCCTACCATGTGCCACAGGGTGTGTGTGTGTTTGTAAA
ACTAGAGTTGCTAAGGATAAGTTTAAAGACCAATACCCCTGTACTTAATCCTGTGCTGTCGAGGGATGGATATATGAAGTAAGGTGAGAT
CCTTAACCTTTCAAAATTTTCGGGTTCCAGGGAGACACACAAGCGAGGGTTTTGTGGTGCCTGGAGCCTGTGTCCTGCCCTGCTACAGTA
GTGATTAATAGTGTCATGGTAGCTAAAGGAGAAAAAGGGGGTTTCGTTTACACGCTGTGAGATCACCGCAAACCTACCTTACTGTGTTGA
AACGGGACAAATGCAATAGAACGCATTGGGTGGTGTGTGTCTGATCCTGGGTTCTTGTCTCCCCTAAATGCTGCCCCCCAAGTTACTGTA
TTTGTCTGGGCTTTGTAGGACTTCACTACGTTGATTGCTAGGTGGCCTAGTTTGTGTAAATATAATGTATTGGTCTTTCTCCGTGTTCTT
TGGGGGTTTTGTTTACAAACTTCTTTTTGTATTGAGAGAAAAATAGCCAAAGCATCTTTGACAGAAGGTTCTGCACCAGGCAAAAAGATC
TGAAACATTAGTTTGGGGGGCCCTCTTCTTAAAGTGGGGATCTTGAACCATCCTTTCTTTTGTATTCCCCTTCCCCTATTACCTATTAGA
CCAGATCTTCTGTCCTAAAAACTTGTCTTCTACCCTGCCCTCTTTTCTGTTCACCCCCAAAAGAAAACTTACACACCCACACACATACAC
ATTTCATGCTTGGAGTGTCTCCACAACTCTTAAATGATGTATGCAAAAATACTGAAGCTAGGAAAACCCTCCATCCCTTGTTCCCAACCT
CCTAAGTCAAGACCATTACCATTTCTTTCTTTCTTTTTTTTTTTTTTTTAAAATGGAGTCTCACTGTGTCACCCAGGCTGGAGTGCAGTG
GCATGATCGGCTCACTGCAGCCTCTGCCTCTTGGGTTCAAGTGATTCTCCTGCCTCAGCCTCCTGAGTAGCTGGGATTTCAGGCACCCGC
CACACTCAGCTAATTTTTGTATTTTTAGTAGAGACGGGGTTTCACCATGTTGTCCAGGCTGGTCTGGAACTCCTGACCTCAGGTGATCTG
CCCACCTTGGCTTCCCAAAGTGCTGGGATTACAGGCATGAGCCACCATGCTGGGCCAACCATTTCTTGGTGTATTCATGCCAAACACTTA
AGACACTGCTGTAGCCCAGGCGCGGTGGCTCACACCTGTAATCCCAGCACTTTGGAAGGCTGAGGCGGGCGGATCACAAGGTCACGAGTT
CAAAACTATCCTGGCCAACACAGTGAAACCCCGTCTCTACTAAAATACAAAAAAATTAGCCGGGTGTGGTGGTGCATGCCTTTAGTCCTA
GCTATTCAGGAGGCTGAGGCAGGGGAATCGCTTGAACCCGAGAGGCAGAGGTTGCAGTGAGCTGAGATCGCACCACTGCACTCCAGCCTG
GTTACAGAGCAAGACTCTGTCTCAAACAAAACAAAACAAAACAAAAACACACTACTGTATTTTGGATGGATCAAACCTCCTTAATTTTAA
TTTCTAATCCTAAAGTAAAGAGATGCAATTGGGGGCCTTCCATGTAGAAAGTGGGGTCAGGAGGCCAAGAAAGGGAATATGAATGTATAT
CCAAGTCACTCAGGAACTTTTATGCAGGTGCTAGAAACTTTATGTCAAAGTGGCCACAAGATTGTTTAATAGGAGACGAACGAATGTAAC
TCCATGTTTACTGCTAAAAACCAAAGCTTTGTGTAAAATCTTGAATTTATGGGGCGGGAGGGTAGGAAAGCCTGTACCTGTCTGTTTTTT
TCCTGATCCTTTTCCCTCATTCCTGAACTGCAGGAGACTGAGCCCCTTTGGGCTTTGGTGACCCCATCACTGGGGTGTGTTTATTTGATG
GTTGATTTTGCTGTACTGGGTACTTCCTTTCCCATTTTCTAATCATTTTTTAACACAAGCTGACTCTTCCCTTCCCTTCTCCTTTCCCTG

>In-frame_ENST00000374166_ENST00000326279_TCGA-55-8085-01A_RPS6KA1_chr1_26885428_+_LIN28A_chr1_26751794_length(amino acids)=527AA_start in transcript=143_stop in transcript=1726
MPLAQLKEPWPLMELVPLDPENGQTSGEEAGLQPSKDEGVLKEISITHHVKAGSEKADPSHFELLKVLGQGSFGKVFLVRKVTRPDSGHL
YAMKVLKKATLKVRDRVRTKMERDILADVNHPFVVKLHYAFQTEGKLYLILDFLRGGDLFTRLSKEVMFTEEDVKFYLAELALGLDHLHS
LGIIYRDLKPENILLDEEGHIKLTDFGLSKEAIDHEKKAYSFCGTVEYMAPEVVNRQGHSHSADWWSYGVLMGKDRKETMTLILKAKLGM
PQFLSTEAQSLLRALFKRNPANRLGSGPDGAEEIKRHVFYSTIDWNKLYRREIKPPFKPAVAQPDDTFYFDTEFTSRTPKDSPGIPPSAG
AHQLFRGFSFVATGLMEDDGKPRAPQAPLHSVVQSKLHMEGFRSLKEGEAVEFTFKKSAKGLESIRVTGPGGVFCIGSERRPKGKSMQKR

--------------------------------------------------------------
>In-frame_ENST00000374166_ENST00000254231_TCGA-55-8085-01A_RPS6KA1_chr1_26885428_+_LIN28A_chr1_26751794_length(transcript)=4480nt_BP=1325nt
GCGGCGGCGGCGGACGGCCCAGCCGGAGCGCGAGGGGCTCGGGGGGGCGCGGCGGTTCGGGTCGCAGAGCCAGGGACCCCAGGACCCGGG
AGGCGGCGCAGCCGGGGCCGCCGGAGGAGCGCGGGTGACCTGGCGGCGGCGAGATGCCGCTCGCCCAGCTCAAGGAGCCCTGGCCGCTCA
TGGAGCTAGTGCCTCTGGACCCGGAGAATGGACAGACCTCAGGGGAAGAAGCTGGACTTCAGCCGTCCAAGGATGAGGGCGTCCTCAAGG
AGATCTCCATCACGCACCACGTCAAGGCTGGCTCTGAGAAGGCTGATCCATCCCATTTCGAGCTCCTCAAGGTTCTGGGCCAGGGATCCT
TTGGCAAAGTCTTCCTGGTGCGGAAAGTCACCCGGCCTGACAGTGGGCACCTGTATGCTATGAAGGTGCTGAAGAAGGCAACGCTGAAAG
TACGTGACCGCGTCCGGACCAAGATGGAGAGAGACATCCTGGCTGATGTAAATCACCCATTCGTGGTGAAGCTGCACTATGCCTTCCAGA
CCGAGGGCAAGCTCTATCTCATTCTGGACTTCCTGCGTGGTGGGGACCTCTTCACCCGGCTCTCAAAAGAGGTGATGTTCACGGAGGAGG
ATGTGAAGTTTTACCTGGCTGAGCTGGCTCTGGGCCTGGATCACCTGCACAGCCTGGGTATCATTTACAGAGACCTCAAGCCTGAGAACA
TCCTTCTGGATGAGGAGGGCCACATCAAACTCACTGACTTTGGCCTGAGCAAAGAGGCCATTGACCACGAGAAGAAGGCCTATTCTTTCT
GCGGGACAGTGGAGTACATGGCCCCTGAGGTCGTCAACCGCCAGGGCCACTCCCATAGTGCGGACTGGTGGTCCTATGGGGTGTTGATGG
GGAAGGACCGGAAGGAGACCATGACACTGATTCTGAAGGCGAAGCTAGGCATGCCCCAGTTTCTGAGCACTGAAGCCCAGAGCCTCTTGC
GGGCCCTGTTCAAGCGGAATCCTGCCAACCGGCTCGGCTCCGGCCCTGATGGGGCAGAGGAAATCAAGCGGCATGTCTTCTACTCCACCA
TTGACTGGAATAAGCTATACCGTCGTGAGATCAAGCCACCCTTCAAGCCAGCAGTGGCTCAGCCTGATGACACCTTCTACTTTGACACCG
AGTTCACGTCCCGCACACCCAAGGATTCCCCAGGCATCCCCCCCAGCGCTGGGGCCCATCAGCTGTTCCGGGGCTTCAGCTTCGTGGCCA
CCGGCCTGATGGAAGACGACGGCAAGCCTCGTGCCCCGCAGGCACCCCTGCACTCGGTGGTACAGAGTAAGCTGCACATGGAAGGGTTCC
GGAGCTTGAAGGAGGGTGAGGCAGTGGAGTTCACCTTTAAGAAGTCAGCCAAGGGTCTGGAATCCATCCGTGTCACCGGACCTGGTGGAG
TATTCTGTATTGGGAGTGAGAGGCGGCCAAAAGGAAAGAGCATGCAGAAGCGCAGATCAAAAGGAGACAGGTGCTACAACTGTGGAGGTC
TAGATCATCATGCCAAGGAATGCAAGCTGCCACCCCAGCCCAAGAAGTGCCACTTCTGCCAGAGCATCAGCCATATGGTAGCCTCATGTC
CGCTGAAGGCCCAGCAGGGCCCTAGTGCACAGGGAAAGCCAACCTACTTTCGAGAGGAAGAAGAAGAAATCCACAGCCCTACCCTGCTCC
CGGAGGCACAGAATTGAGCCACAATGGGTGGGGGCTATTCTTTTGCTATCAGGAAGTTTTGAGGAGCAGGCAGAGTGGAGAAAGTGGGAA
TAGGGTGCATTGGGGCTAGTTGGCACTGCCATGTATCTCAGGCTTGGGTTCACACCATCACCCTTTCTTCCCTCTAGGTGGGGGGAAAGG
GTGAGTCAAAGGAACTCCAACCATGCTCTGTCCAAATGCAAGTGAGGGTTCTGGGGGCAACCAGGAGGGGGGAATCACCCTACAACCTGC
ATACTTTGAGTCTCCATCCCCAGAATTTCCAGCTTTTGAAAGTGGCCTGGATAGGGAAGTTGTTTTCCTTTTAAAGAAGGATATATAATA
ATTCCCATGCCAGAGTGAAATGATTAAGTATAAGACCAGATTCATGGAGCCAAGCCACTACATTCTGTGGAAGGAGATCTCTCAGGAGTA
AGCATTGTTTTTTTTTCACATCTTGTATCCTCATACCCACTTTTGGGATAGGGTGCTGGCAGCTGTCCCAAGCAATGGGTAATGATGATG
GCAAAAAGGGTGTTTGGGGGAACAGCTGCAGACCTGCTGCTCTATGCTCACCCCCGCCCCATTCTGGGCCAATGTGATTTTATTTATTTG
CTCCCTTGGATACTGCACCTTGGGTCCCACTTTCTCCAGGATGCCAACTGCACTAGCTGTGTGCGAATGACGTATCTTGTGCATTTTAAC
TTTTTTTCCTTAATATAAATATTCTGGTTTTGTATTTTTGTATATTTTAATCTAAGGCCCTCATTTCCTGCACTGTGTTCTCAGGTACAT
GAGCAATCTCAGGGATAGCCAGCAGCAGCTCCAGGTCTGCGCAGCAGGAATTACTTTTTGTTGTTTTTGCCACCGTGGAGAGCAACTATT
TGGAGTGCACAGCCTATTGAACTACCTCATTTTTGCCAATAAGAGCTGGCTTTTCTGCCATAGTGTCCTCTTGAAACCCCCTCTGCCTTG
AAAATGTTTTATGGGAGACTAGGTTTTAACTGGGTGGCCCCATGACTTGATTGCCTTCTACTGGAAGATTGGGAATTAGTCTAAACAGGA
AATGGTGGTACACAGAGGCTAGGAGAGGCTGGGCCCGGTGAAAAGGCCAGAGAGCAAGCCAAGATTAGGTGAGGGTTGTCTAATCCTATG
GCACAGGACGTGCTTTACATCTCCAGATCTGTTCTTCACCAGATTAGGTTAGGCCTACCATGTGCCACAGGGTGTGTGTGTGTTTGTAAA
ACTAGAGTTGCTAAGGATAAGTTTAAAGACCAATACCCCTGTACTTAATCCTGTGCTGTCGAGGGATGGATATATGAAGTAAGGTGAGAT
CCTTAACCTTTCAAAATTTTCGGGTTCCAGGGAGACACACAAGCGAGGGTTTTGTGGTGCCTGGAGCCTGTGTCCTGCCCTGCTACAGTA
GTGATTAATAGTGTCATGGTAGCTAAAGGAGAAAAAGGGGGTTTCGTTTACACGCTGTGAGATCACCGCAAACCTACCTTACTGTGTTGA
AACGGGACAAATGCAATAGAACGCATTGGGTGGTGTGTGTCTGATCCTGGGTTCTTGTCTCCCCTAAATGCTGCCCCCCAAGTTACTGTA
TTTGTCTGGGCTTTGTAGGACTTCACTACGTTGATTGCTAGGTGGCCTAGTTTGTGTAAATATAATGTATTGGTCTTTCTCCGTGTTCTT
TGGGGGTTTTGTTTACAAACTTCTTTTTGTATTGAGAGAAAAATAGCCAAAGCATCTTTGACAGAAGGTTCTGCACCAGGCAAAAAGATC
TGAAACATTAGTTTGGGGGGCCCTCTTCTTAAAGTGGGGATCTTGAACCATCCTTTCTTTTGTATTCCCCTTCCCCTATTACCTATTAGA
CCAGATCTTCTGTCCTAAAAACTTGTCTTCTACCCTGCCCTCTTTTCTGTTCACCCCCAAAAGAAAACTTACACACCCACACACATACAC
ATTTCATGCTTGGAGTGTCTCCACAACTCTTAAATGATGTATGCAAAAATACTGAAGCTAGGAAAACCCTCCATCCCTTGTTCCCAACCT
CCTAAGTCAAGACCATTACCATTTCTTTCTTTCTTTTTTTTTTTTTTTTAAAATGGAGTCTCACTGTGTCACCCAGGCAGAGGTTGCAGT
GAGCTGAGATCGCACCACTGCACTCCAGCCTGGTTACAGAGCAAGACTCTGTCTCAAACAAAACAAAACAAAACAAAAACACACTACTGT
ATTTTGGATGGATCAAACCTCCTTAATTTTAATTTCTAATCCTAAAGTAAAGAGATGCAATTGGGGGCCTTCCATGTAGAAAGTGGGGTC
AGGAGGCCAAGAAAGGGAATATGAATGTATATCCAAGTCACTCAGGAACTTTTATGCAGGTGCTAGAAACTTTATGTCAAAGTGGCCACA
AGATTGTTTAATAGGAGACGAACGAATGTAACTCCATGTTTACTGCTAAAAACCAAAGCTTTGTGTAAAATCTTGAATTTATGGGGCGGG
AGGGTAGGAAAGCCTGTACCTGTCTGTTTTTTTCCTGATCCTTTTCCCTCATTCCTGAACTGCAGGAGACTGAGCCCCTTTGGGCTTTGG
TGACCCCATCACTGGGGTGTGTTTATTTGATGGTTGATTTTGCTGTACTGGGTACTTCCTTTCCCATTTTCTAATCATTTTTTAACACAA

>In-frame_ENST00000374166_ENST00000254231_TCGA-55-8085-01A_RPS6KA1_chr1_26885428_+_LIN28A_chr1_26751794_length(amino acids)=527AA_start in transcript=143_stop in transcript=1726
MPLAQLKEPWPLMELVPLDPENGQTSGEEAGLQPSKDEGVLKEISITHHVKAGSEKADPSHFELLKVLGQGSFGKVFLVRKVTRPDSGHL
YAMKVLKKATLKVRDRVRTKMERDILADVNHPFVVKLHYAFQTEGKLYLILDFLRGGDLFTRLSKEVMFTEEDVKFYLAELALGLDHLHS
LGIIYRDLKPENILLDEEGHIKLTDFGLSKEAIDHEKKAYSFCGTVEYMAPEVVNRQGHSHSADWWSYGVLMGKDRKETMTLILKAKLGM
PQFLSTEAQSLLRALFKRNPANRLGSGPDGAEEIKRHVFYSTIDWNKLYRREIKPPFKPAVAQPDDTFYFDTEFTSRTPKDSPGIPPSAG
AHQLFRGFSFVATGLMEDDGKPRAPQAPLHSVVQSKLHMEGFRSLKEGEAVEFTFKKSAKGLESIRVTGPGGVFCIGSERRPKGKSMQKR

--------------------------------------------------------------
>In-frame_ENST00000526792_ENST00000326279_TCGA-55-8085-01A_RPS6KA1_chr1_26885428_+_LIN28A_chr1_26751794_length(transcript)=5039nt_BP=1373nt
AAATTGCTCTGGGATTCCTGCCATGGGGATTTTACAGGCACTGCTAGGCTGCTCAGAGGTTCTCTCGCTGACTCACCCTCTGAGGTGAGG
AACACTGCCCTCTGCAGCCCCTGTACTGCCACCCCGCTACTAGGACCGGGGCGCAAGGTCCTGGGGGTGGTGCTCAGAGGTGGATTCCTT
TGCCCAGGCACGCCCCTGGGACCAGGCCTCCAAAGGAGAAGAATGGACAGACCTCAGGGGAAGAAGCTGGACTTCAGCCGTCCAAGGATG
AGGGCGTCCTCAAGGAGATCTCCATCACGCACCACGTCAAGGCTGGCTCTGAGAAGGCTGATCCATCCCATTTCGAGCTCCTCAAGGTTC
TGGGCCAGGGATCCTTTGGCAAAGTCTTCCTGGTGCGGAAAGTCACCCGGCCTGACAGTGGGCACCTGTATGCTATGAAGGTGCTGAAGA
AGGCAACGCTGAAAGTACGTGACCGCGTCCGGACCAAGATGGAGAGAGACATCCTGGCTGATGTAAATCACCCATTCGTGGTGAAGCTGC
ACTATGCCTTCCAGACCGAGGGCAAGCTCTATCTCATTCTGGACTTCCTGCGTGGTGGGGACCTCTTCACCCGGCTCTCAAAAGAGGTGA
TGTTCACGGAGGAGGATGTGAAGTTTTACCTGGCTGAGCTGGCTCTGGGCCTGGATCACCTGCACAGCCTGGGTATCATTTACAGAGACC
TCAAGCCTGAGAACATCCTTCTGGATGAGGAGGGCCACATCAAACTCACTGACTTTGGCCTGAGCAAAGAGGCCATTGACCACGAGAAGA
AGGCCTATTCTTTCTGCGGGACAGTGGAGTACATGGCCCCTGAGGTCGTCAACCGCCAGGGCCACTCCCATAGTGCGGACTGGTGGTCCT
ATGGGGTGTTGATGTTTGAGATGCTGACGGGCTCCCTGCCCTTCCAGGGGAAGGACCGGAAGGAGACCATGACACTGATTCTGAAGGCGA
AGCTAGGCATGCCCCAGTTTCTGAGCACTGAAGCCCAGAGCCTCTTGCGGGCCCTGTTCAAGCGGAATCCTGCCAACCGGCTCGGCTCCG
GCCCTGATGGGGCAGAGGAAATCAAGCGGCATGTCTTCTACTCCACCATTGACTGGAATAAGCTATACCGTCGTGAGATCAAGCCACCCT
TCAAGCCAGCAGTGGCTCAGCCTGATGACACCTTCTACTTTGACACCGAGTTCACGTCCCGCACACCCAAGGATTCCCCAGGCATCCCCC
CCAGCGCTGGGGCCCATCAGCTGTTCCGGGGCTTCAGCTTCGTGGCCACCGGCCTGATGGAAGACGACGGCAAGCCTCGTGCCCCGCAGG
CACCCCTGCACTCGGTGGTACAGAGTAAGCTGCACATGGAAGGGTTCCGGAGCTTGAAGGAGGGTGAGGCAGTGGAGTTCACCTTTAAGA
AGTCAGCCAAGGGTCTGGAATCCATCCGTGTCACCGGACCTGGTGGAGTATTCTGTATTGGGAGTGAGAGGCGGCCAAAAGGAAAGAGCA
TGCAGAAGCGCAGATCAAAAGGAGACAGGTGCTACAACTGTGGAGGTCTAGATCATCATGCCAAGGAATGCAAGCTGCCACCCCAGCCCA
AGAAGTGCCACTTCTGCCAGAGCATCAGCCATATGGTAGCCTCATGTCCGCTGAAGGCCCAGCAGGGCCCTAGTGCACAGGGAAAGCCAA
CCTACTTTCGAGAGGAAGAAGAAGAAATCCACAGCCCTACCCTGCTCCCGGAGGCACAGAATTGAGCCACAATGGGTGGGGGCTATTCTT
TTGCTATCAGGAAGTTTTGAGGAGCAGGCAGAGTGGAGAAAGTGGGAATAGGGTGCATTGGGGCTAGTTGGCACTGCCATGTATCTCAGG
CTTGGGTTCACACCATCACCCTTTCTTCCCTCTAGGTGGGGGGAAAGGGTGAGTCAAAGGAACTCCAACCATGCTCTGTCCAAATGCAAG
TGAGGGTTCTGGGGGCAACCAGGAGGGGGGAATCACCCTACAACCTGCATACTTTGAGTCTCCATCCCCAGAATTTCCAGCTTTTGAAAG
TGGCCTGGATAGGGAAGTTGTTTTCCTTTTAAAGAAGGATATATAATAATTCCCATGCCAGAGTGAAATGATTAAGTATAAGACCAGATT
CATGGAGCCAAGCCACTACATTCTGTGGAAGGAGATCTCTCAGGAGTAAGCATTGTTTTTTTTTCACATCTTGTATCCTCATACCCACTT
TTGGGATAGGGTGCTGGCAGCTGTCCCAAGCAATGGGTAATGATGATGGCAAAAAGGGTGTTTGGGGGAACAGCTGCAGACCTGCTGCTC
TATGCTCACCCCCGCCCCATTCTGGGCCAATGTGATTTTATTTATTTGCTCCCTTGGATACTGCACCTTGGGTCCCACTTTCTCCAGGAT
GCCAACTGCACTAGCTGTGTGCGAATGACGTATCTTGTGCATTTTAACTTTTTTTCCTTAATATAAATATTCTGGTTTTGTATTTTTGTA
TATTTTAATCTAAGGCCCTCATTTCCTGCACTGTGTTCTCAGGTACATGAGCAATCTCAGGGATAGCCAGCAGCAGCTCCAGGTCTGCGC
AGCAGGAATTACTTTTTGTTGTTTTTGCCACCGTGGAGAGCAACTATTTGGAGTGCACAGCCTATTGAACTACCTCATTTTTGCCAATAA
GAGCTGGCTTTTCTGCCATAGTGTCCTCTTGAAACCCCCTCTGCCTTGAAAATGTTTTATGGGAGACTAGGTTTTAACTGGGTGGCCCCA
TGACTTGATTGCCTTCTACTGGAAGATTGGGAATTAGTCTAAACAGGAAATGGTGGTACACAGAGGCTAGGAGAGGCTGGGCCCGGTGAA
AAGGCCAGAGAGCAAGCCAAGATTAGGTGAGGGTTGTCTAATCCTATGGCACAGGACGTGCTTTACATCTCCAGATCTGTTCTTCACCAG
ATTAGGTTAGGCCTACCATGTGCCACAGGGTGTGTGTGTGTTTGTAAAACTAGAGTTGCTAAGGATAAGTTTAAAGACCAATACCCCTGT
ACTTAATCCTGTGCTGTCGAGGGATGGATATATGAAGTAAGGTGAGATCCTTAACCTTTCAAAATTTTCGGGTTCCAGGGAGACACACAA
GCGAGGGTTTTGTGGTGCCTGGAGCCTGTGTCCTGCCCTGCTACAGTAGTGATTAATAGTGTCATGGTAGCTAAAGGAGAAAAAGGGGGT
TTCGTTTACACGCTGTGAGATCACCGCAAACCTACCTTACTGTGTTGAAACGGGACAAATGCAATAGAACGCATTGGGTGGTGTGTGTCT
GATCCTGGGTTCTTGTCTCCCCTAAATGCTGCCCCCCAAGTTACTGTATTTGTCTGGGCTTTGTAGGACTTCACTACGTTGATTGCTAGG
TGGCCTAGTTTGTGTAAATATAATGTATTGGTCTTTCTCCGTGTTCTTTGGGGGTTTTGTTTACAAACTTCTTTTTGTATTGAGAGAAAA
ATAGCCAAAGCATCTTTGACAGAAGGTTCTGCACCAGGCAAAAAGATCTGAAACATTAGTTTGGGGGGCCCTCTTCTTAAAGTGGGGATC
TTGAACCATCCTTTCTTTTGTATTCCCCTTCCCCTATTACCTATTAGACCAGATCTTCTGTCCTAAAAACTTGTCTTCTACCCTGCCCTC
TTTTCTGTTCACCCCCAAAAGAAAACTTACACACCCACACACATACACATTTCATGCTTGGAGTGTCTCCACAACTCTTAAATGATGTAT
GCAAAAATACTGAAGCTAGGAAAACCCTCCATCCCTTGTTCCCAACCTCCTAAGTCAAGACCATTACCATTTCTTTCTTTCTTTTTTTTT
TTTTTTTAAAATGGAGTCTCACTGTGTCACCCAGGCTGGAGTGCAGTGGCATGATCGGCTCACTGCAGCCTCTGCCTCTTGGGTTCAAGT
GATTCTCCTGCCTCAGCCTCCTGAGTAGCTGGGATTTCAGGCACCCGCCACACTCAGCTAATTTTTGTATTTTTAGTAGAGACGGGGTTT
CACCATGTTGTCCAGGCTGGTCTGGAACTCCTGACCTCAGGTGATCTGCCCACCTTGGCTTCCCAAAGTGCTGGGATTACAGGCATGAGC
CACCATGCTGGGCCAACCATTTCTTGGTGTATTCATGCCAAACACTTAAGACACTGCTGTAGCCCAGGCGCGGTGGCTCACACCTGTAAT
CCCAGCACTTTGGAAGGCTGAGGCGGGCGGATCACAAGGTCACGAGTTCAAAACTATCCTGGCCAACACAGTGAAACCCCGTCTCTACTA
AAATACAAAAAAATTAGCCGGGTGTGGTGGTGCATGCCTTTAGTCCTAGCTATTCAGGAGGCTGAGGCAGGGGAATCGCTTGAACCCGAG
AGGCAGAGGTTGCAGTGAGCTGAGATCGCACCACTGCACTCCAGCCTGGTTACAGAGCAAGACTCTGTCTCAAACAAAACAAAACAAAAC
AAAAACACACTACTGTATTTTGGATGGATCAAACCTCCTTAATTTTAATTTCTAATCCTAAAGTAAAGAGATGCAATTGGGGGCCTTCCA
TGTAGAAAGTGGGGTCAGGAGGCCAAGAAAGGGAATATGAATGTATATCCAAGTCACTCAGGAACTTTTATGCAGGTGCTAGAAACTTTA
TGTCAAAGTGGCCACAAGATTGTTTAATAGGAGACGAACGAATGTAACTCCATGTTTACTGCTAAAAACCAAAGCTTTGTGTAAAATCTT
GAATTTATGGGGCGGGAGGGTAGGAAAGCCTGTACCTGTCTGTTTTTTTCCTGATCCTTTTCCCTCATTCCTGAACTGCAGGAGACTGAG
CCCCTTTGGGCTTTGGTGACCCCATCACTGGGGTGTGTTTATTTGATGGTTGATTTTGCTGTACTGGGTACTTCCTTTCCCATTTTCTAA

>In-frame_ENST00000526792_ENST00000326279_TCGA-55-8085-01A_RPS6KA1_chr1_26885428_+_LIN28A_chr1_26751794_length(amino acids)=557AA_start in transcript=101_stop in transcript=1774
MQPLYCHPATRTGAQGPGGGAQRWIPLPRHAPGTRPPKEKNGQTSGEEAGLQPSKDEGVLKEISITHHVKAGSEKADPSHFELLKVLGQG
SFGKVFLVRKVTRPDSGHLYAMKVLKKATLKVRDRVRTKMERDILADVNHPFVVKLHYAFQTEGKLYLILDFLRGGDLFTRLSKEVMFTE
EDVKFYLAELALGLDHLHSLGIIYRDLKPENILLDEEGHIKLTDFGLSKEAIDHEKKAYSFCGTVEYMAPEVVNRQGHSHSADWWSYGVL
MFEMLTGSLPFQGKDRKETMTLILKAKLGMPQFLSTEAQSLLRALFKRNPANRLGSGPDGAEEIKRHVFYSTIDWNKLYRREIKPPFKPA
VAQPDDTFYFDTEFTSRTPKDSPGIPPSAGAHQLFRGFSFVATGLMEDDGKPRAPQAPLHSVVQSKLHMEGFRSLKEGEAVEFTFKKSAK
GLESIRVTGPGGVFCIGSERRPKGKSMQKRRSKGDRCYNCGGLDHHAKECKLPPQPKKCHFCQSISHMVASCPLKAQQGPSAQGKPTYFR

--------------------------------------------------------------
>In-frame_ENST00000526792_ENST00000254231_TCGA-55-8085-01A_RPS6KA1_chr1_26885428_+_LIN28A_chr1_26751794_length(transcript)=4528nt_BP=1373nt
AAATTGCTCTGGGATTCCTGCCATGGGGATTTTACAGGCACTGCTAGGCTGCTCAGAGGTTCTCTCGCTGACTCACCCTCTGAGGTGAGG
AACACTGCCCTCTGCAGCCCCTGTACTGCCACCCCGCTACTAGGACCGGGGCGCAAGGTCCTGGGGGTGGTGCTCAGAGGTGGATTCCTT
TGCCCAGGCACGCCCCTGGGACCAGGCCTCCAAAGGAGAAGAATGGACAGACCTCAGGGGAAGAAGCTGGACTTCAGCCGTCCAAGGATG
AGGGCGTCCTCAAGGAGATCTCCATCACGCACCACGTCAAGGCTGGCTCTGAGAAGGCTGATCCATCCCATTTCGAGCTCCTCAAGGTTC
TGGGCCAGGGATCCTTTGGCAAAGTCTTCCTGGTGCGGAAAGTCACCCGGCCTGACAGTGGGCACCTGTATGCTATGAAGGTGCTGAAGA
AGGCAACGCTGAAAGTACGTGACCGCGTCCGGACCAAGATGGAGAGAGACATCCTGGCTGATGTAAATCACCCATTCGTGGTGAAGCTGC
ACTATGCCTTCCAGACCGAGGGCAAGCTCTATCTCATTCTGGACTTCCTGCGTGGTGGGGACCTCTTCACCCGGCTCTCAAAAGAGGTGA
TGTTCACGGAGGAGGATGTGAAGTTTTACCTGGCTGAGCTGGCTCTGGGCCTGGATCACCTGCACAGCCTGGGTATCATTTACAGAGACC
TCAAGCCTGAGAACATCCTTCTGGATGAGGAGGGCCACATCAAACTCACTGACTTTGGCCTGAGCAAAGAGGCCATTGACCACGAGAAGA
AGGCCTATTCTTTCTGCGGGACAGTGGAGTACATGGCCCCTGAGGTCGTCAACCGCCAGGGCCACTCCCATAGTGCGGACTGGTGGTCCT
ATGGGGTGTTGATGTTTGAGATGCTGACGGGCTCCCTGCCCTTCCAGGGGAAGGACCGGAAGGAGACCATGACACTGATTCTGAAGGCGA
AGCTAGGCATGCCCCAGTTTCTGAGCACTGAAGCCCAGAGCCTCTTGCGGGCCCTGTTCAAGCGGAATCCTGCCAACCGGCTCGGCTCCG
GCCCTGATGGGGCAGAGGAAATCAAGCGGCATGTCTTCTACTCCACCATTGACTGGAATAAGCTATACCGTCGTGAGATCAAGCCACCCT
TCAAGCCAGCAGTGGCTCAGCCTGATGACACCTTCTACTTTGACACCGAGTTCACGTCCCGCACACCCAAGGATTCCCCAGGCATCCCCC
CCAGCGCTGGGGCCCATCAGCTGTTCCGGGGCTTCAGCTTCGTGGCCACCGGCCTGATGGAAGACGACGGCAAGCCTCGTGCCCCGCAGG
CACCCCTGCACTCGGTGGTACAGAGTAAGCTGCACATGGAAGGGTTCCGGAGCTTGAAGGAGGGTGAGGCAGTGGAGTTCACCTTTAAGA
AGTCAGCCAAGGGTCTGGAATCCATCCGTGTCACCGGACCTGGTGGAGTATTCTGTATTGGGAGTGAGAGGCGGCCAAAAGGAAAGAGCA
TGCAGAAGCGCAGATCAAAAGGAGACAGGTGCTACAACTGTGGAGGTCTAGATCATCATGCCAAGGAATGCAAGCTGCCACCCCAGCCCA
AGAAGTGCCACTTCTGCCAGAGCATCAGCCATATGGTAGCCTCATGTCCGCTGAAGGCCCAGCAGGGCCCTAGTGCACAGGGAAAGCCAA
CCTACTTTCGAGAGGAAGAAGAAGAAATCCACAGCCCTACCCTGCTCCCGGAGGCACAGAATTGAGCCACAATGGGTGGGGGCTATTCTT
TTGCTATCAGGAAGTTTTGAGGAGCAGGCAGAGTGGAGAAAGTGGGAATAGGGTGCATTGGGGCTAGTTGGCACTGCCATGTATCTCAGG
CTTGGGTTCACACCATCACCCTTTCTTCCCTCTAGGTGGGGGGAAAGGGTGAGTCAAAGGAACTCCAACCATGCTCTGTCCAAATGCAAG
TGAGGGTTCTGGGGGCAACCAGGAGGGGGGAATCACCCTACAACCTGCATACTTTGAGTCTCCATCCCCAGAATTTCCAGCTTTTGAAAG
TGGCCTGGATAGGGAAGTTGTTTTCCTTTTAAAGAAGGATATATAATAATTCCCATGCCAGAGTGAAATGATTAAGTATAAGACCAGATT
CATGGAGCCAAGCCACTACATTCTGTGGAAGGAGATCTCTCAGGAGTAAGCATTGTTTTTTTTTCACATCTTGTATCCTCATACCCACTT
TTGGGATAGGGTGCTGGCAGCTGTCCCAAGCAATGGGTAATGATGATGGCAAAAAGGGTGTTTGGGGGAACAGCTGCAGACCTGCTGCTC
TATGCTCACCCCCGCCCCATTCTGGGCCAATGTGATTTTATTTATTTGCTCCCTTGGATACTGCACCTTGGGTCCCACTTTCTCCAGGAT
GCCAACTGCACTAGCTGTGTGCGAATGACGTATCTTGTGCATTTTAACTTTTTTTCCTTAATATAAATATTCTGGTTTTGTATTTTTGTA
TATTTTAATCTAAGGCCCTCATTTCCTGCACTGTGTTCTCAGGTACATGAGCAATCTCAGGGATAGCCAGCAGCAGCTCCAGGTCTGCGC
AGCAGGAATTACTTTTTGTTGTTTTTGCCACCGTGGAGAGCAACTATTTGGAGTGCACAGCCTATTGAACTACCTCATTTTTGCCAATAA
GAGCTGGCTTTTCTGCCATAGTGTCCTCTTGAAACCCCCTCTGCCTTGAAAATGTTTTATGGGAGACTAGGTTTTAACTGGGTGGCCCCA
TGACTTGATTGCCTTCTACTGGAAGATTGGGAATTAGTCTAAACAGGAAATGGTGGTACACAGAGGCTAGGAGAGGCTGGGCCCGGTGAA
AAGGCCAGAGAGCAAGCCAAGATTAGGTGAGGGTTGTCTAATCCTATGGCACAGGACGTGCTTTACATCTCCAGATCTGTTCTTCACCAG
ATTAGGTTAGGCCTACCATGTGCCACAGGGTGTGTGTGTGTTTGTAAAACTAGAGTTGCTAAGGATAAGTTTAAAGACCAATACCCCTGT
ACTTAATCCTGTGCTGTCGAGGGATGGATATATGAAGTAAGGTGAGATCCTTAACCTTTCAAAATTTTCGGGTTCCAGGGAGACACACAA
GCGAGGGTTTTGTGGTGCCTGGAGCCTGTGTCCTGCCCTGCTACAGTAGTGATTAATAGTGTCATGGTAGCTAAAGGAGAAAAAGGGGGT
TTCGTTTACACGCTGTGAGATCACCGCAAACCTACCTTACTGTGTTGAAACGGGACAAATGCAATAGAACGCATTGGGTGGTGTGTGTCT
GATCCTGGGTTCTTGTCTCCCCTAAATGCTGCCCCCCAAGTTACTGTATTTGTCTGGGCTTTGTAGGACTTCACTACGTTGATTGCTAGG
TGGCCTAGTTTGTGTAAATATAATGTATTGGTCTTTCTCCGTGTTCTTTGGGGGTTTTGTTTACAAACTTCTTTTTGTATTGAGAGAAAA
ATAGCCAAAGCATCTTTGACAGAAGGTTCTGCACCAGGCAAAAAGATCTGAAACATTAGTTTGGGGGGCCCTCTTCTTAAAGTGGGGATC
TTGAACCATCCTTTCTTTTGTATTCCCCTTCCCCTATTACCTATTAGACCAGATCTTCTGTCCTAAAAACTTGTCTTCTACCCTGCCCTC
TTTTCTGTTCACCCCCAAAAGAAAACTTACACACCCACACACATACACATTTCATGCTTGGAGTGTCTCCACAACTCTTAAATGATGTAT
GCAAAAATACTGAAGCTAGGAAAACCCTCCATCCCTTGTTCCCAACCTCCTAAGTCAAGACCATTACCATTTCTTTCTTTCTTTTTTTTT
TTTTTTTAAAATGGAGTCTCACTGTGTCACCCAGGCAGAGGTTGCAGTGAGCTGAGATCGCACCACTGCACTCCAGCCTGGTTACAGAGC
AAGACTCTGTCTCAAACAAAACAAAACAAAACAAAAACACACTACTGTATTTTGGATGGATCAAACCTCCTTAATTTTAATTTCTAATCC
TAAAGTAAAGAGATGCAATTGGGGGCCTTCCATGTAGAAAGTGGGGTCAGGAGGCCAAGAAAGGGAATATGAATGTATATCCAAGTCACT
CAGGAACTTTTATGCAGGTGCTAGAAACTTTATGTCAAAGTGGCCACAAGATTGTTTAATAGGAGACGAACGAATGTAACTCCATGTTTA
CTGCTAAAAACCAAAGCTTTGTGTAAAATCTTGAATTTATGGGGCGGGAGGGTAGGAAAGCCTGTACCTGTCTGTTTTTTTCCTGATCCT
TTTCCCTCATTCCTGAACTGCAGGAGACTGAGCCCCTTTGGGCTTTGGTGACCCCATCACTGGGGTGTGTTTATTTGATGGTTGATTTTG
CTGTACTGGGTACTTCCTTTCCCATTTTCTAATCATTTTTTAACACAAGCTGACTCTTCCCTTCCCTTCTCCTTTCCCTGGGAAAATACA

>In-frame_ENST00000526792_ENST00000254231_TCGA-55-8085-01A_RPS6KA1_chr1_26885428_+_LIN28A_chr1_26751794_length(amino acids)=557AA_start in transcript=101_stop in transcript=1774
MQPLYCHPATRTGAQGPGGGAQRWIPLPRHAPGTRPPKEKNGQTSGEEAGLQPSKDEGVLKEISITHHVKAGSEKADPSHFELLKVLGQG
SFGKVFLVRKVTRPDSGHLYAMKVLKKATLKVRDRVRTKMERDILADVNHPFVVKLHYAFQTEGKLYLILDFLRGGDLFTRLSKEVMFTE
EDVKFYLAELALGLDHLHSLGIIYRDLKPENILLDEEGHIKLTDFGLSKEAIDHEKKAYSFCGTVEYMAPEVVNRQGHSHSADWWSYGVL
MFEMLTGSLPFQGKDRKETMTLILKAKLGMPQFLSTEAQSLLRALFKRNPANRLGSGPDGAEEIKRHVFYSTIDWNKLYRREIKPPFKPA
VAQPDDTFYFDTEFTSRTPKDSPGIPPSAGAHQLFRGFSFVATGLMEDDGKPRAPQAPLHSVVQSKLHMEGFRSLKEGEAVEFTFKKSAK
GLESIRVTGPGGVFCIGSERRPKGKSMQKRRSKGDRCYNCGGLDHHAKECKLPPQPKKCHFCQSISHMVASCPLKAQQGPSAQGKPTYFR

--------------------------------------------------------------
>In-frame_ENST00000374162_ENST00000326279_TCGA-55-8085-01A_RPS6KA1_chr1_26885428_+_LIN28A_chr1_26751794_length(transcript)=4812nt_BP=1146nt
CTGTACTGCCACCCCGCTACTAGGACCGGGGCGCAAGGTCCTGGGGGTGGTGCTCAGAGGTGGATTCCTTTGCCCAGGCACGCCCCTGGG
ACCAGGCCTCCAAAGGAGAAGAATGGACAGACCTCAGGGGAAGAAGCTGGACTTCAGCCGTCCAAGGTCTTCCTGGTGCGGAAAGTCACC
CGGCCTGACAGTGGGCACCTGTATGCTATGAAGGTGCTGAAGAAGGCAACGCTGAAAGTACGTGACCGCGTCCGGACCAAGATGGAGAGA
GACATCCTGGCTGATGTAAATCACCCATTCGTGGTGAAGCTGCACTATGCCTTCCAGACCGAGGGCAAGCTCTATCTCATTCTGGACTTC
CTGCGTGGTGGGGACCTCTTCACCCGGCTCTCAAAAGAGGTGATGTTCACGGAGGAGGATGTGAAGTTTTACCTGGCTGAGCTGGCTCTG
GGCCTGGATCACCTGCACAGCCTGGGTATCATTTACAGAGACCTCAAGCCTGAGAACATCCTTCTGGATGAGGAGGGCCACATCAAACTC
ACTGACTTTGGCCTGAGCAAAGAGGCCATTGACCACGAGAAGAAGGCCTATTCTTTCTGCGGGACAGTGGAGTACATGGCCCCTGAGGTC
GTCAACCGCCAGGGCCACTCCCATAGTGCGGACTGGTGGTCCTATGGGGTGTTGATGTTTGAGATGCTGACGGGCTCCCTGCCCTTCCAG
GGGAAGGACCGGAAGGAGACCATGACACTGATTCTGAAGGCGAAGCTAGGCATGCCCCAGTTTCTGAGCACTGAAGCCCAGAGCCTCTTG
CGGGCCCTGTTCAAGCGGAATCCTGCCAACCGGCTCGGCTCCGGCCCTGATGGGGCAGAGGAAATCAAGCGGCATGTCTTCTACTCCACC
ATTGACTGGAATAAGCTATACCGTCGTGAGATCAAGCCACCCTTCAAGCCAGCAGTGGCTCAGCCTGATGACACCTTCTACTTTGACACC
GAGTTCACGTCCCGCACACCCAAGGATTCCCCAGGCATCCCCCCCAGCGCTGGGGCCCATCAGCTGTTCCGGGGCTTCAGCTTCGTGGCC
ACCGGCCTGATGGAAGACGACGGCAAGCCTCGTGCCCCGCAGGCACCCCTGCACTCGGTGGTACAGAGTAAGCTGCACATGGAAGGGTTC
CGGAGCTTGAAGGAGGGTGAGGCAGTGGAGTTCACCTTTAAGAAGTCAGCCAAGGGTCTGGAATCCATCCGTGTCACCGGACCTGGTGGA
GTATTCTGTATTGGGAGTGAGAGGCGGCCAAAAGGAAAGAGCATGCAGAAGCGCAGATCAAAAGGAGACAGGTGCTACAACTGTGGAGGT
CTAGATCATCATGCCAAGGAATGCAAGCTGCCACCCCAGCCCAAGAAGTGCCACTTCTGCCAGAGCATCAGCCATATGGTAGCCTCATGT
CCGCTGAAGGCCCAGCAGGGCCCTAGTGCACAGGGAAAGCCAACCTACTTTCGAGAGGAAGAAGAAGAAATCCACAGCCCTACCCTGCTC
CCGGAGGCACAGAATTGAGCCACAATGGGTGGGGGCTATTCTTTTGCTATCAGGAAGTTTTGAGGAGCAGGCAGAGTGGAGAAAGTGGGA
ATAGGGTGCATTGGGGCTAGTTGGCACTGCCATGTATCTCAGGCTTGGGTTCACACCATCACCCTTTCTTCCCTCTAGGTGGGGGGAAAG
GGTGAGTCAAAGGAACTCCAACCATGCTCTGTCCAAATGCAAGTGAGGGTTCTGGGGGCAACCAGGAGGGGGGAATCACCCTACAACCTG
CATACTTTGAGTCTCCATCCCCAGAATTTCCAGCTTTTGAAAGTGGCCTGGATAGGGAAGTTGTTTTCCTTTTAAAGAAGGATATATAAT
AATTCCCATGCCAGAGTGAAATGATTAAGTATAAGACCAGATTCATGGAGCCAAGCCACTACATTCTGTGGAAGGAGATCTCTCAGGAGT
AAGCATTGTTTTTTTTTCACATCTTGTATCCTCATACCCACTTTTGGGATAGGGTGCTGGCAGCTGTCCCAAGCAATGGGTAATGATGAT
GGCAAAAAGGGTGTTTGGGGGAACAGCTGCAGACCTGCTGCTCTATGCTCACCCCCGCCCCATTCTGGGCCAATGTGATTTTATTTATTT
GCTCCCTTGGATACTGCACCTTGGGTCCCACTTTCTCCAGGATGCCAACTGCACTAGCTGTGTGCGAATGACGTATCTTGTGCATTTTAA
CTTTTTTTCCTTAATATAAATATTCTGGTTTTGTATTTTTGTATATTTTAATCTAAGGCCCTCATTTCCTGCACTGTGTTCTCAGGTACA
TGAGCAATCTCAGGGATAGCCAGCAGCAGCTCCAGGTCTGCGCAGCAGGAATTACTTTTTGTTGTTTTTGCCACCGTGGAGAGCAACTAT
TTGGAGTGCACAGCCTATTGAACTACCTCATTTTTGCCAATAAGAGCTGGCTTTTCTGCCATAGTGTCCTCTTGAAACCCCCTCTGCCTT
GAAAATGTTTTATGGGAGACTAGGTTTTAACTGGGTGGCCCCATGACTTGATTGCCTTCTACTGGAAGATTGGGAATTAGTCTAAACAGG
AAATGGTGGTACACAGAGGCTAGGAGAGGCTGGGCCCGGTGAAAAGGCCAGAGAGCAAGCCAAGATTAGGTGAGGGTTGTCTAATCCTAT
GGCACAGGACGTGCTTTACATCTCCAGATCTGTTCTTCACCAGATTAGGTTAGGCCTACCATGTGCCACAGGGTGTGTGTGTGTTTGTAA
AACTAGAGTTGCTAAGGATAAGTTTAAAGACCAATACCCCTGTACTTAATCCTGTGCTGTCGAGGGATGGATATATGAAGTAAGGTGAGA
TCCTTAACCTTTCAAAATTTTCGGGTTCCAGGGAGACACACAAGCGAGGGTTTTGTGGTGCCTGGAGCCTGTGTCCTGCCCTGCTACAGT
AGTGATTAATAGTGTCATGGTAGCTAAAGGAGAAAAAGGGGGTTTCGTTTACACGCTGTGAGATCACCGCAAACCTACCTTACTGTGTTG
AAACGGGACAAATGCAATAGAACGCATTGGGTGGTGTGTGTCTGATCCTGGGTTCTTGTCTCCCCTAAATGCTGCCCCCCAAGTTACTGT
ATTTGTCTGGGCTTTGTAGGACTTCACTACGTTGATTGCTAGGTGGCCTAGTTTGTGTAAATATAATGTATTGGTCTTTCTCCGTGTTCT
TTGGGGGTTTTGTTTACAAACTTCTTTTTGTATTGAGAGAAAAATAGCCAAAGCATCTTTGACAGAAGGTTCTGCACCAGGCAAAAAGAT
CTGAAACATTAGTTTGGGGGGCCCTCTTCTTAAAGTGGGGATCTTGAACCATCCTTTCTTTTGTATTCCCCTTCCCCTATTACCTATTAG
ACCAGATCTTCTGTCCTAAAAACTTGTCTTCTACCCTGCCCTCTTTTCTGTTCACCCCCAAAAGAAAACTTACACACCCACACACATACA
CATTTCATGCTTGGAGTGTCTCCACAACTCTTAAATGATGTATGCAAAAATACTGAAGCTAGGAAAACCCTCCATCCCTTGTTCCCAACC
TCCTAAGTCAAGACCATTACCATTTCTTTCTTTCTTTTTTTTTTTTTTTTAAAATGGAGTCTCACTGTGTCACCCAGGCTGGAGTGCAGT
GGCATGATCGGCTCACTGCAGCCTCTGCCTCTTGGGTTCAAGTGATTCTCCTGCCTCAGCCTCCTGAGTAGCTGGGATTTCAGGCACCCG
CCACACTCAGCTAATTTTTGTATTTTTAGTAGAGACGGGGTTTCACCATGTTGTCCAGGCTGGTCTGGAACTCCTGACCTCAGGTGATCT
GCCCACCTTGGCTTCCCAAAGTGCTGGGATTACAGGCATGAGCCACCATGCTGGGCCAACCATTTCTTGGTGTATTCATGCCAAACACTT
AAGACACTGCTGTAGCCCAGGCGCGGTGGCTCACACCTGTAATCCCAGCACTTTGGAAGGCTGAGGCGGGCGGATCACAAGGTCACGAGT
TCAAAACTATCCTGGCCAACACAGTGAAACCCCGTCTCTACTAAAATACAAAAAAATTAGCCGGGTGTGGTGGTGCATGCCTTTAGTCCT
AGCTATTCAGGAGGCTGAGGCAGGGGAATCGCTTGAACCCGAGAGGCAGAGGTTGCAGTGAGCTGAGATCGCACCACTGCACTCCAGCCT
GGTTACAGAGCAAGACTCTGTCTCAAACAAAACAAAACAAAACAAAAACACACTACTGTATTTTGGATGGATCAAACCTCCTTAATTTTA
ATTTCTAATCCTAAAGTAAAGAGATGCAATTGGGGGCCTTCCATGTAGAAAGTGGGGTCAGGAGGCCAAGAAAGGGAATATGAATGTATA
TCCAAGTCACTCAGGAACTTTTATGCAGGTGCTAGAAACTTTATGTCAAAGTGGCCACAAGATTGTTTAATAGGAGACGAACGAATGTAA
CTCCATGTTTACTGCTAAAAACCAAAGCTTTGTGTAAAATCTTGAATTTATGGGGCGGGAGGGTAGGAAAGCCTGTACCTGTCTGTTTTT
TTCCTGATCCTTTTCCCTCATTCCTGAACTGCAGGAGACTGAGCCCCTTTGGGCTTTGGTGACCCCATCACTGGGGTGTGTTTATTTGAT
GGTTGATTTTGCTGTACTGGGTACTTCCTTTCCCATTTTCTAATCATTTTTTAACACAAGCTGACTCTTCCCTTCCCTTCTCCTTTCCCT

>In-frame_ENST00000374162_ENST00000326279_TCGA-55-8085-01A_RPS6KA1_chr1_26885428_+_LIN28A_chr1_26751794_length(amino acids)=515AA_start in transcript=0_stop in transcript=1547
LYCHPATRTGAQGPGGGAQRWIPLPRHAPGTRPPKEKNGQTSGEEAGLQPSKVFLVRKVTRPDSGHLYAMKVLKKATLKVRDRVRTKMER
DILADVNHPFVVKLHYAFQTEGKLYLILDFLRGGDLFTRLSKEVMFTEEDVKFYLAELALGLDHLHSLGIIYRDLKPENILLDEEGHIKL
TDFGLSKEAIDHEKKAYSFCGTVEYMAPEVVNRQGHSHSADWWSYGVLMFEMLTGSLPFQGKDRKETMTLILKAKLGMPQFLSTEAQSLL
RALFKRNPANRLGSGPDGAEEIKRHVFYSTIDWNKLYRREIKPPFKPAVAQPDDTFYFDTEFTSRTPKDSPGIPPSAGAHQLFRGFSFVA
TGLMEDDGKPRAPQAPLHSVVQSKLHMEGFRSLKEGEAVEFTFKKSAKGLESIRVTGPGGVFCIGSERRPKGKSMQKRRSKGDRCYNCGG

--------------------------------------------------------------
>In-frame_ENST00000374162_ENST00000254231_TCGA-55-8085-01A_RPS6KA1_chr1_26885428_+_LIN28A_chr1_26751794_length(transcript)=4301nt_BP=1146nt
CTGTACTGCCACCCCGCTACTAGGACCGGGGCGCAAGGTCCTGGGGGTGGTGCTCAGAGGTGGATTCCTTTGCCCAGGCACGCCCCTGGG
ACCAGGCCTCCAAAGGAGAAGAATGGACAGACCTCAGGGGAAGAAGCTGGACTTCAGCCGTCCAAGGTCTTCCTGGTGCGGAAAGTCACC
CGGCCTGACAGTGGGCACCTGTATGCTATGAAGGTGCTGAAGAAGGCAACGCTGAAAGTACGTGACCGCGTCCGGACCAAGATGGAGAGA
GACATCCTGGCTGATGTAAATCACCCATTCGTGGTGAAGCTGCACTATGCCTTCCAGACCGAGGGCAAGCTCTATCTCATTCTGGACTTC
CTGCGTGGTGGGGACCTCTTCACCCGGCTCTCAAAAGAGGTGATGTTCACGGAGGAGGATGTGAAGTTTTACCTGGCTGAGCTGGCTCTG
GGCCTGGATCACCTGCACAGCCTGGGTATCATTTACAGAGACCTCAAGCCTGAGAACATCCTTCTGGATGAGGAGGGCCACATCAAACTC
ACTGACTTTGGCCTGAGCAAAGAGGCCATTGACCACGAGAAGAAGGCCTATTCTTTCTGCGGGACAGTGGAGTACATGGCCCCTGAGGTC
GTCAACCGCCAGGGCCACTCCCATAGTGCGGACTGGTGGTCCTATGGGGTGTTGATGTTTGAGATGCTGACGGGCTCCCTGCCCTTCCAG
GGGAAGGACCGGAAGGAGACCATGACACTGATTCTGAAGGCGAAGCTAGGCATGCCCCAGTTTCTGAGCACTGAAGCCCAGAGCCTCTTG
CGGGCCCTGTTCAAGCGGAATCCTGCCAACCGGCTCGGCTCCGGCCCTGATGGGGCAGAGGAAATCAAGCGGCATGTCTTCTACTCCACC
ATTGACTGGAATAAGCTATACCGTCGTGAGATCAAGCCACCCTTCAAGCCAGCAGTGGCTCAGCCTGATGACACCTTCTACTTTGACACC
GAGTTCACGTCCCGCACACCCAAGGATTCCCCAGGCATCCCCCCCAGCGCTGGGGCCCATCAGCTGTTCCGGGGCTTCAGCTTCGTGGCC
ACCGGCCTGATGGAAGACGACGGCAAGCCTCGTGCCCCGCAGGCACCCCTGCACTCGGTGGTACAGAGTAAGCTGCACATGGAAGGGTTC
CGGAGCTTGAAGGAGGGTGAGGCAGTGGAGTTCACCTTTAAGAAGTCAGCCAAGGGTCTGGAATCCATCCGTGTCACCGGACCTGGTGGA
GTATTCTGTATTGGGAGTGAGAGGCGGCCAAAAGGAAAGAGCATGCAGAAGCGCAGATCAAAAGGAGACAGGTGCTACAACTGTGGAGGT
CTAGATCATCATGCCAAGGAATGCAAGCTGCCACCCCAGCCCAAGAAGTGCCACTTCTGCCAGAGCATCAGCCATATGGTAGCCTCATGT
CCGCTGAAGGCCCAGCAGGGCCCTAGTGCACAGGGAAAGCCAACCTACTTTCGAGAGGAAGAAGAAGAAATCCACAGCCCTACCCTGCTC
CCGGAGGCACAGAATTGAGCCACAATGGGTGGGGGCTATTCTTTTGCTATCAGGAAGTTTTGAGGAGCAGGCAGAGTGGAGAAAGTGGGA
ATAGGGTGCATTGGGGCTAGTTGGCACTGCCATGTATCTCAGGCTTGGGTTCACACCATCACCCTTTCTTCCCTCTAGGTGGGGGGAAAG
GGTGAGTCAAAGGAACTCCAACCATGCTCTGTCCAAATGCAAGTGAGGGTTCTGGGGGCAACCAGGAGGGGGGAATCACCCTACAACCTG
CATACTTTGAGTCTCCATCCCCAGAATTTCCAGCTTTTGAAAGTGGCCTGGATAGGGAAGTTGTTTTCCTTTTAAAGAAGGATATATAAT
AATTCCCATGCCAGAGTGAAATGATTAAGTATAAGACCAGATTCATGGAGCCAAGCCACTACATTCTGTGGAAGGAGATCTCTCAGGAGT
AAGCATTGTTTTTTTTTCACATCTTGTATCCTCATACCCACTTTTGGGATAGGGTGCTGGCAGCTGTCCCAAGCAATGGGTAATGATGAT
GGCAAAAAGGGTGTTTGGGGGAACAGCTGCAGACCTGCTGCTCTATGCTCACCCCCGCCCCATTCTGGGCCAATGTGATTTTATTTATTT
GCTCCCTTGGATACTGCACCTTGGGTCCCACTTTCTCCAGGATGCCAACTGCACTAGCTGTGTGCGAATGACGTATCTTGTGCATTTTAA
CTTTTTTTCCTTAATATAAATATTCTGGTTTTGTATTTTTGTATATTTTAATCTAAGGCCCTCATTTCCTGCACTGTGTTCTCAGGTACA
TGAGCAATCTCAGGGATAGCCAGCAGCAGCTCCAGGTCTGCGCAGCAGGAATTACTTTTTGTTGTTTTTGCCACCGTGGAGAGCAACTAT
TTGGAGTGCACAGCCTATTGAACTACCTCATTTTTGCCAATAAGAGCTGGCTTTTCTGCCATAGTGTCCTCTTGAAACCCCCTCTGCCTT
GAAAATGTTTTATGGGAGACTAGGTTTTAACTGGGTGGCCCCATGACTTGATTGCCTTCTACTGGAAGATTGGGAATTAGTCTAAACAGG
AAATGGTGGTACACAGAGGCTAGGAGAGGCTGGGCCCGGTGAAAAGGCCAGAGAGCAAGCCAAGATTAGGTGAGGGTTGTCTAATCCTAT
GGCACAGGACGTGCTTTACATCTCCAGATCTGTTCTTCACCAGATTAGGTTAGGCCTACCATGTGCCACAGGGTGTGTGTGTGTTTGTAA
AACTAGAGTTGCTAAGGATAAGTTTAAAGACCAATACCCCTGTACTTAATCCTGTGCTGTCGAGGGATGGATATATGAAGTAAGGTGAGA
TCCTTAACCTTTCAAAATTTTCGGGTTCCAGGGAGACACACAAGCGAGGGTTTTGTGGTGCCTGGAGCCTGTGTCCTGCCCTGCTACAGT
AGTGATTAATAGTGTCATGGTAGCTAAAGGAGAAAAAGGGGGTTTCGTTTACACGCTGTGAGATCACCGCAAACCTACCTTACTGTGTTG
AAACGGGACAAATGCAATAGAACGCATTGGGTGGTGTGTGTCTGATCCTGGGTTCTTGTCTCCCCTAAATGCTGCCCCCCAAGTTACTGT
ATTTGTCTGGGCTTTGTAGGACTTCACTACGTTGATTGCTAGGTGGCCTAGTTTGTGTAAATATAATGTATTGGTCTTTCTCCGTGTTCT
TTGGGGGTTTTGTTTACAAACTTCTTTTTGTATTGAGAGAAAAATAGCCAAAGCATCTTTGACAGAAGGTTCTGCACCAGGCAAAAAGAT
CTGAAACATTAGTTTGGGGGGCCCTCTTCTTAAAGTGGGGATCTTGAACCATCCTTTCTTTTGTATTCCCCTTCCCCTATTACCTATTAG
ACCAGATCTTCTGTCCTAAAAACTTGTCTTCTACCCTGCCCTCTTTTCTGTTCACCCCCAAAAGAAAACTTACACACCCACACACATACA
CATTTCATGCTTGGAGTGTCTCCACAACTCTTAAATGATGTATGCAAAAATACTGAAGCTAGGAAAACCCTCCATCCCTTGTTCCCAACC
TCCTAAGTCAAGACCATTACCATTTCTTTCTTTCTTTTTTTTTTTTTTTTAAAATGGAGTCTCACTGTGTCACCCAGGCAGAGGTTGCAG
TGAGCTGAGATCGCACCACTGCACTCCAGCCTGGTTACAGAGCAAGACTCTGTCTCAAACAAAACAAAACAAAACAAAAACACACTACTG
TATTTTGGATGGATCAAACCTCCTTAATTTTAATTTCTAATCCTAAAGTAAAGAGATGCAATTGGGGGCCTTCCATGTAGAAAGTGGGGT
CAGGAGGCCAAGAAAGGGAATATGAATGTATATCCAAGTCACTCAGGAACTTTTATGCAGGTGCTAGAAACTTTATGTCAAAGTGGCCAC
AAGATTGTTTAATAGGAGACGAACGAATGTAACTCCATGTTTACTGCTAAAAACCAAAGCTTTGTGTAAAATCTTGAATTTATGGGGCGG
GAGGGTAGGAAAGCCTGTACCTGTCTGTTTTTTTCCTGATCCTTTTCCCTCATTCCTGAACTGCAGGAGACTGAGCCCCTTTGGGCTTTG
GTGACCCCATCACTGGGGTGTGTTTATTTGATGGTTGATTTTGCTGTACTGGGTACTTCCTTTCCCATTTTCTAATCATTTTTTAACACA

>In-frame_ENST00000374162_ENST00000254231_TCGA-55-8085-01A_RPS6KA1_chr1_26885428_+_LIN28A_chr1_26751794_length(amino acids)=515AA_start in transcript=0_stop in transcript=1547
LYCHPATRTGAQGPGGGAQRWIPLPRHAPGTRPPKEKNGQTSGEEAGLQPSKVFLVRKVTRPDSGHLYAMKVLKKATLKVRDRVRTKMER
DILADVNHPFVVKLHYAFQTEGKLYLILDFLRGGDLFTRLSKEVMFTEEDVKFYLAELALGLDHLHSLGIIYRDLKPENILLDEEGHIKL
TDFGLSKEAIDHEKKAYSFCGTVEYMAPEVVNRQGHSHSADWWSYGVLMFEMLTGSLPFQGKDRKETMTLILKAKLGMPQFLSTEAQSLL
RALFKRNPANRLGSGPDGAEEIKRHVFYSTIDWNKLYRREIKPPFKPAVAQPDDTFYFDTEFTSRTPKDSPGIPPSAGAHQLFRGFSFVA
TGLMEDDGKPRAPQAPLHSVVQSKLHMEGFRSLKEGEAVEFTFKKSAKGLESIRVTGPGGVFCIGSERRPKGKSMQKRRSKGDRCYNCGG

--------------------------------------------------------------
>In-frame_ENST00000530003_ENST00000326279_TCGA-55-8085-01A_RPS6KA1_chr1_26885428_+_LIN28A_chr1_26751794_length(transcript)=4883nt_BP=1217nt
AGAAGTAGGAAGAGTAACGGGGCCCTCTGGTCACCGGCTTGAGCCCCACCATGCAGACCCCAGCAGATTTCCCCAGGGTTGAGAGAGACT
TGGTTCCCTGTCCCAGAAAGGATGAGGGCGTCCTCAAGGAGATCTCCATCACGCACCACGTCAAGGCTGGCTCTGAGAAGGCTGATCCAT
CCCATTTCGAGCTCCTCAAGGTTCTGGGCCAGGGATCCTTTGGCAAAGTCTTCCTGGTGCGGAAAGTCACCCGGCCTGACAGTGGGCACC
TGTATGCTATGAAGGTGCTGAAGAAGGCAACGCTGAAAGTACGTGACCGCGTCCGGACCAAGATGGAGAGAGACATCCTGGCTGATGTAA
ATCACCCATTCGTGGTGAAGCTGCACTATGCCTTCCAGACCGAGGGCAAGCTCTATCTCATTCTGGACTTCCTGCGTGGTGGGGACCTCT
TCACCCGGCTCTCAAAAGAGGTGATGTTCACGGAGGAGGATGTGAAGTTTTACCTGGCTGAGCTGGCTCTGGGCCTGGATCACCTGCACA
GCCTGGGTATCATTTACAGAGACCTCAAGCCTGAGAACATCCTTCTGGATGAGGAGGGCCACATCAAACTCACTGACTTTGGCCTGAGCA
AAGAGGCCATTGACCACGAGAAGAAGGCCTATTCTTTCTGCGGGACAGTGGAGTACATGGCCCCTGAGGTCGTCAACCGCCAGGGCCACT
CCCATAGTGCGGACTGGTGGTCCTATGGGGTGTTGATGTTTGAGATGCTGACGGGCTCCCTGCCCTTCCAGGGGAAGGACCGGAAGGAGA
CCATGACACTGATTCTGAAGGCGAAGCTAGGCATGCCCCAGTTTCTGAGCACTGAAGCCCAGAGCCTCTTGCGGGCCCTGTTCAAGCGGA
ATCCTGCCAACCGGCTCGGCTCCGGCCCTGATGGGGCAGAGGAAATCAAGCGGCATGTCTTCTACTCCACCATTGACTGGAATAAGCTAT
ACCGTCGTGAGATCAAGCCACCCTTCAAGCCAGCAGTGGCTCAGCCTGATGACACCTTCTACTTTGACACCGAGTTCACGTCCCGCACAC
CCAAGGATTCCCCAGGCATCCCCCCCAGCGCTGGGGCCCATCAGCTGTTCCGGGGCTTCAGCTTCGTGGCCACCGGCCTGATGGAAGACG
ACGGCAAGCCTCGTGCCCCGCAGGCACCCCTGCACTCGGTGGTACAGAGTAAGCTGCACATGGAAGGGTTCCGGAGCTTGAAGGAGGGTG
AGGCAGTGGAGTTCACCTTTAAGAAGTCAGCCAAGGGTCTGGAATCCATCCGTGTCACCGGACCTGGTGGAGTATTCTGTATTGGGAGTG
AGAGGCGGCCAAAAGGAAAGAGCATGCAGAAGCGCAGATCAAAAGGAGACAGGTGCTACAACTGTGGAGGTCTAGATCATCATGCCAAGG
AATGCAAGCTGCCACCCCAGCCCAAGAAGTGCCACTTCTGCCAGAGCATCAGCCATATGGTAGCCTCATGTCCGCTGAAGGCCCAGCAGG
GCCCTAGTGCACAGGGAAAGCCAACCTACTTTCGAGAGGAAGAAGAAGAAATCCACAGCCCTACCCTGCTCCCGGAGGCACAGAATTGAG
CCACAATGGGTGGGGGCTATTCTTTTGCTATCAGGAAGTTTTGAGGAGCAGGCAGAGTGGAGAAAGTGGGAATAGGGTGCATTGGGGCTA
GTTGGCACTGCCATGTATCTCAGGCTTGGGTTCACACCATCACCCTTTCTTCCCTCTAGGTGGGGGGAAAGGGTGAGTCAAAGGAACTCC
AACCATGCTCTGTCCAAATGCAAGTGAGGGTTCTGGGGGCAACCAGGAGGGGGGAATCACCCTACAACCTGCATACTTTGAGTCTCCATC
CCCAGAATTTCCAGCTTTTGAAAGTGGCCTGGATAGGGAAGTTGTTTTCCTTTTAAAGAAGGATATATAATAATTCCCATGCCAGAGTGA
AATGATTAAGTATAAGACCAGATTCATGGAGCCAAGCCACTACATTCTGTGGAAGGAGATCTCTCAGGAGTAAGCATTGTTTTTTTTTCA
CATCTTGTATCCTCATACCCACTTTTGGGATAGGGTGCTGGCAGCTGTCCCAAGCAATGGGTAATGATGATGGCAAAAAGGGTGTTTGGG
GGAACAGCTGCAGACCTGCTGCTCTATGCTCACCCCCGCCCCATTCTGGGCCAATGTGATTTTATTTATTTGCTCCCTTGGATACTGCAC
CTTGGGTCCCACTTTCTCCAGGATGCCAACTGCACTAGCTGTGTGCGAATGACGTATCTTGTGCATTTTAACTTTTTTTCCTTAATATAA
ATATTCTGGTTTTGTATTTTTGTATATTTTAATCTAAGGCCCTCATTTCCTGCACTGTGTTCTCAGGTACATGAGCAATCTCAGGGATAG
CCAGCAGCAGCTCCAGGTCTGCGCAGCAGGAATTACTTTTTGTTGTTTTTGCCACCGTGGAGAGCAACTATTTGGAGTGCACAGCCTATT
GAACTACCTCATTTTTGCCAATAAGAGCTGGCTTTTCTGCCATAGTGTCCTCTTGAAACCCCCTCTGCCTTGAAAATGTTTTATGGGAGA
CTAGGTTTTAACTGGGTGGCCCCATGACTTGATTGCCTTCTACTGGAAGATTGGGAATTAGTCTAAACAGGAAATGGTGGTACACAGAGG
CTAGGAGAGGCTGGGCCCGGTGAAAAGGCCAGAGAGCAAGCCAAGATTAGGTGAGGGTTGTCTAATCCTATGGCACAGGACGTGCTTTAC
ATCTCCAGATCTGTTCTTCACCAGATTAGGTTAGGCCTACCATGTGCCACAGGGTGTGTGTGTGTTTGTAAAACTAGAGTTGCTAAGGAT
AAGTTTAAAGACCAATACCCCTGTACTTAATCCTGTGCTGTCGAGGGATGGATATATGAAGTAAGGTGAGATCCTTAACCTTTCAAAATT
TTCGGGTTCCAGGGAGACACACAAGCGAGGGTTTTGTGGTGCCTGGAGCCTGTGTCCTGCCCTGCTACAGTAGTGATTAATAGTGTCATG
GTAGCTAAAGGAGAAAAAGGGGGTTTCGTTTACACGCTGTGAGATCACCGCAAACCTACCTTACTGTGTTGAAACGGGACAAATGCAATA
GAACGCATTGGGTGGTGTGTGTCTGATCCTGGGTTCTTGTCTCCCCTAAATGCTGCCCCCCAAGTTACTGTATTTGTCTGGGCTTTGTAG
GACTTCACTACGTTGATTGCTAGGTGGCCTAGTTTGTGTAAATATAATGTATTGGTCTTTCTCCGTGTTCTTTGGGGGTTTTGTTTACAA
ACTTCTTTTTGTATTGAGAGAAAAATAGCCAAAGCATCTTTGACAGAAGGTTCTGCACCAGGCAAAAAGATCTGAAACATTAGTTTGGGG
GGCCCTCTTCTTAAAGTGGGGATCTTGAACCATCCTTTCTTTTGTATTCCCCTTCCCCTATTACCTATTAGACCAGATCTTCTGTCCTAA
AAACTTGTCTTCTACCCTGCCCTCTTTTCTGTTCACCCCCAAAAGAAAACTTACACACCCACACACATACACATTTCATGCTTGGAGTGT
CTCCACAACTCTTAAATGATGTATGCAAAAATACTGAAGCTAGGAAAACCCTCCATCCCTTGTTCCCAACCTCCTAAGTCAAGACCATTA
CCATTTCTTTCTTTCTTTTTTTTTTTTTTTTAAAATGGAGTCTCACTGTGTCACCCAGGCTGGAGTGCAGTGGCATGATCGGCTCACTGC
AGCCTCTGCCTCTTGGGTTCAAGTGATTCTCCTGCCTCAGCCTCCTGAGTAGCTGGGATTTCAGGCACCCGCCACACTCAGCTAATTTTT
GTATTTTTAGTAGAGACGGGGTTTCACCATGTTGTCCAGGCTGGTCTGGAACTCCTGACCTCAGGTGATCTGCCCACCTTGGCTTCCCAA
AGTGCTGGGATTACAGGCATGAGCCACCATGCTGGGCCAACCATTTCTTGGTGTATTCATGCCAAACACTTAAGACACTGCTGTAGCCCA
GGCGCGGTGGCTCACACCTGTAATCCCAGCACTTTGGAAGGCTGAGGCGGGCGGATCACAAGGTCACGAGTTCAAAACTATCCTGGCCAA
CACAGTGAAACCCCGTCTCTACTAAAATACAAAAAAATTAGCCGGGTGTGGTGGTGCATGCCTTTAGTCCTAGCTATTCAGGAGGCTGAG
GCAGGGGAATCGCTTGAACCCGAGAGGCAGAGGTTGCAGTGAGCTGAGATCGCACCACTGCACTCCAGCCTGGTTACAGAGCAAGACTCT
GTCTCAAACAAAACAAAACAAAACAAAAACACACTACTGTATTTTGGATGGATCAAACCTCCTTAATTTTAATTTCTAATCCTAAAGTAA
AGAGATGCAATTGGGGGCCTTCCATGTAGAAAGTGGGGTCAGGAGGCCAAGAAAGGGAATATGAATGTATATCCAAGTCACTCAGGAACT
TTTATGCAGGTGCTAGAAACTTTATGTCAAAGTGGCCACAAGATTGTTTAATAGGAGACGAACGAATGTAACTCCATGTTTACTGCTAAA
AACCAAAGCTTTGTGTAAAATCTTGAATTTATGGGGCGGGAGGGTAGGAAAGCCTGTACCTGTCTGTTTTTTTCCTGATCCTTTTCCCTC
ATTCCTGAACTGCAGGAGACTGAGCCCCTTTGGGCTTTGGTGACCCCATCACTGGGGTGTGTTTATTTGATGGTTGATTTTGCTGTACTG
GGTACTTCCTTTCCCATTTTCTAATCATTTTTTAACACAAGCTGACTCTTCCCTTCCCTTCTCCTTTCCCTGGGAAAATACAATGAATAA

>In-frame_ENST00000530003_ENST00000326279_TCGA-55-8085-01A_RPS6KA1_chr1_26885428_+_LIN28A_chr1_26751794_length(amino acids)=530AA_start in transcript=26_stop in transcript=1618
MVTGLSPTMQTPADFPRVERDLVPCPRKDEGVLKEISITHHVKAGSEKADPSHFELLKVLGQGSFGKVFLVRKVTRPDSGHLYAMKVLKK
ATLKVRDRVRTKMERDILADVNHPFVVKLHYAFQTEGKLYLILDFLRGGDLFTRLSKEVMFTEEDVKFYLAELALGLDHLHSLGIIYRDL
KPENILLDEEGHIKLTDFGLSKEAIDHEKKAYSFCGTVEYMAPEVVNRQGHSHSADWWSYGVLMFEMLTGSLPFQGKDRKETMTLILKAK
LGMPQFLSTEAQSLLRALFKRNPANRLGSGPDGAEEIKRHVFYSTIDWNKLYRREIKPPFKPAVAQPDDTFYFDTEFTSRTPKDSPGIPP
SAGAHQLFRGFSFVATGLMEDDGKPRAPQAPLHSVVQSKLHMEGFRSLKEGEAVEFTFKKSAKGLESIRVTGPGGVFCIGSERRPKGKSM

--------------------------------------------------------------
>In-frame_ENST00000530003_ENST00000254231_TCGA-55-8085-01A_RPS6KA1_chr1_26885428_+_LIN28A_chr1_26751794_length(transcript)=4372nt_BP=1217nt
AGAAGTAGGAAGAGTAACGGGGCCCTCTGGTCACCGGCTTGAGCCCCACCATGCAGACCCCAGCAGATTTCCCCAGGGTTGAGAGAGACT
TGGTTCCCTGTCCCAGAAAGGATGAGGGCGTCCTCAAGGAGATCTCCATCACGCACCACGTCAAGGCTGGCTCTGAGAAGGCTGATCCAT
CCCATTTCGAGCTCCTCAAGGTTCTGGGCCAGGGATCCTTTGGCAAAGTCTTCCTGGTGCGGAAAGTCACCCGGCCTGACAGTGGGCACC
TGTATGCTATGAAGGTGCTGAAGAAGGCAACGCTGAAAGTACGTGACCGCGTCCGGACCAAGATGGAGAGAGACATCCTGGCTGATGTAA
ATCACCCATTCGTGGTGAAGCTGCACTATGCCTTCCAGACCGAGGGCAAGCTCTATCTCATTCTGGACTTCCTGCGTGGTGGGGACCTCT
TCACCCGGCTCTCAAAAGAGGTGATGTTCACGGAGGAGGATGTGAAGTTTTACCTGGCTGAGCTGGCTCTGGGCCTGGATCACCTGCACA
GCCTGGGTATCATTTACAGAGACCTCAAGCCTGAGAACATCCTTCTGGATGAGGAGGGCCACATCAAACTCACTGACTTTGGCCTGAGCA
AAGAGGCCATTGACCACGAGAAGAAGGCCTATTCTTTCTGCGGGACAGTGGAGTACATGGCCCCTGAGGTCGTCAACCGCCAGGGCCACT
CCCATAGTGCGGACTGGTGGTCCTATGGGGTGTTGATGTTTGAGATGCTGACGGGCTCCCTGCCCTTCCAGGGGAAGGACCGGAAGGAGA
CCATGACACTGATTCTGAAGGCGAAGCTAGGCATGCCCCAGTTTCTGAGCACTGAAGCCCAGAGCCTCTTGCGGGCCCTGTTCAAGCGGA
ATCCTGCCAACCGGCTCGGCTCCGGCCCTGATGGGGCAGAGGAAATCAAGCGGCATGTCTTCTACTCCACCATTGACTGGAATAAGCTAT
ACCGTCGTGAGATCAAGCCACCCTTCAAGCCAGCAGTGGCTCAGCCTGATGACACCTTCTACTTTGACACCGAGTTCACGTCCCGCACAC
CCAAGGATTCCCCAGGCATCCCCCCCAGCGCTGGGGCCCATCAGCTGTTCCGGGGCTTCAGCTTCGTGGCCACCGGCCTGATGGAAGACG
ACGGCAAGCCTCGTGCCCCGCAGGCACCCCTGCACTCGGTGGTACAGAGTAAGCTGCACATGGAAGGGTTCCGGAGCTTGAAGGAGGGTG
AGGCAGTGGAGTTCACCTTTAAGAAGTCAGCCAAGGGTCTGGAATCCATCCGTGTCACCGGACCTGGTGGAGTATTCTGTATTGGGAGTG
AGAGGCGGCCAAAAGGAAAGAGCATGCAGAAGCGCAGATCAAAAGGAGACAGGTGCTACAACTGTGGAGGTCTAGATCATCATGCCAAGG
AATGCAAGCTGCCACCCCAGCCCAAGAAGTGCCACTTCTGCCAGAGCATCAGCCATATGGTAGCCTCATGTCCGCTGAAGGCCCAGCAGG
GCCCTAGTGCACAGGGAAAGCCAACCTACTTTCGAGAGGAAGAAGAAGAAATCCACAGCCCTACCCTGCTCCCGGAGGCACAGAATTGAG
CCACAATGGGTGGGGGCTATTCTTTTGCTATCAGGAAGTTTTGAGGAGCAGGCAGAGTGGAGAAAGTGGGAATAGGGTGCATTGGGGCTA
GTTGGCACTGCCATGTATCTCAGGCTTGGGTTCACACCATCACCCTTTCTTCCCTCTAGGTGGGGGGAAAGGGTGAGTCAAAGGAACTCC
AACCATGCTCTGTCCAAATGCAAGTGAGGGTTCTGGGGGCAACCAGGAGGGGGGAATCACCCTACAACCTGCATACTTTGAGTCTCCATC
CCCAGAATTTCCAGCTTTTGAAAGTGGCCTGGATAGGGAAGTTGTTTTCCTTTTAAAGAAGGATATATAATAATTCCCATGCCAGAGTGA
AATGATTAAGTATAAGACCAGATTCATGGAGCCAAGCCACTACATTCTGTGGAAGGAGATCTCTCAGGAGTAAGCATTGTTTTTTTTTCA
CATCTTGTATCCTCATACCCACTTTTGGGATAGGGTGCTGGCAGCTGTCCCAAGCAATGGGTAATGATGATGGCAAAAAGGGTGTTTGGG
GGAACAGCTGCAGACCTGCTGCTCTATGCTCACCCCCGCCCCATTCTGGGCCAATGTGATTTTATTTATTTGCTCCCTTGGATACTGCAC
CTTGGGTCCCACTTTCTCCAGGATGCCAACTGCACTAGCTGTGTGCGAATGACGTATCTTGTGCATTTTAACTTTTTTTCCTTAATATAA
ATATTCTGGTTTTGTATTTTTGTATATTTTAATCTAAGGCCCTCATTTCCTGCACTGTGTTCTCAGGTACATGAGCAATCTCAGGGATAG
CCAGCAGCAGCTCCAGGTCTGCGCAGCAGGAATTACTTTTTGTTGTTTTTGCCACCGTGGAGAGCAACTATTTGGAGTGCACAGCCTATT
GAACTACCTCATTTTTGCCAATAAGAGCTGGCTTTTCTGCCATAGTGTCCTCTTGAAACCCCCTCTGCCTTGAAAATGTTTTATGGGAGA
CTAGGTTTTAACTGGGTGGCCCCATGACTTGATTGCCTTCTACTGGAAGATTGGGAATTAGTCTAAACAGGAAATGGTGGTACACAGAGG
CTAGGAGAGGCTGGGCCCGGTGAAAAGGCCAGAGAGCAAGCCAAGATTAGGTGAGGGTTGTCTAATCCTATGGCACAGGACGTGCTTTAC
ATCTCCAGATCTGTTCTTCACCAGATTAGGTTAGGCCTACCATGTGCCACAGGGTGTGTGTGTGTTTGTAAAACTAGAGTTGCTAAGGAT
AAGTTTAAAGACCAATACCCCTGTACTTAATCCTGTGCTGTCGAGGGATGGATATATGAAGTAAGGTGAGATCCTTAACCTTTCAAAATT
TTCGGGTTCCAGGGAGACACACAAGCGAGGGTTTTGTGGTGCCTGGAGCCTGTGTCCTGCCCTGCTACAGTAGTGATTAATAGTGTCATG
GTAGCTAAAGGAGAAAAAGGGGGTTTCGTTTACACGCTGTGAGATCACCGCAAACCTACCTTACTGTGTTGAAACGGGACAAATGCAATA
GAACGCATTGGGTGGTGTGTGTCTGATCCTGGGTTCTTGTCTCCCCTAAATGCTGCCCCCCAAGTTACTGTATTTGTCTGGGCTTTGTAG
GACTTCACTACGTTGATTGCTAGGTGGCCTAGTTTGTGTAAATATAATGTATTGGTCTTTCTCCGTGTTCTTTGGGGGTTTTGTTTACAA
ACTTCTTTTTGTATTGAGAGAAAAATAGCCAAAGCATCTTTGACAGAAGGTTCTGCACCAGGCAAAAAGATCTGAAACATTAGTTTGGGG
GGCCCTCTTCTTAAAGTGGGGATCTTGAACCATCCTTTCTTTTGTATTCCCCTTCCCCTATTACCTATTAGACCAGATCTTCTGTCCTAA
AAACTTGTCTTCTACCCTGCCCTCTTTTCTGTTCACCCCCAAAAGAAAACTTACACACCCACACACATACACATTTCATGCTTGGAGTGT
CTCCACAACTCTTAAATGATGTATGCAAAAATACTGAAGCTAGGAAAACCCTCCATCCCTTGTTCCCAACCTCCTAAGTCAAGACCATTA
CCATTTCTTTCTTTCTTTTTTTTTTTTTTTTAAAATGGAGTCTCACTGTGTCACCCAGGCAGAGGTTGCAGTGAGCTGAGATCGCACCAC
TGCACTCCAGCCTGGTTACAGAGCAAGACTCTGTCTCAAACAAAACAAAACAAAACAAAAACACACTACTGTATTTTGGATGGATCAAAC
CTCCTTAATTTTAATTTCTAATCCTAAAGTAAAGAGATGCAATTGGGGGCCTTCCATGTAGAAAGTGGGGTCAGGAGGCCAAGAAAGGGA
ATATGAATGTATATCCAAGTCACTCAGGAACTTTTATGCAGGTGCTAGAAACTTTATGTCAAAGTGGCCACAAGATTGTTTAATAGGAGA
CGAACGAATGTAACTCCATGTTTACTGCTAAAAACCAAAGCTTTGTGTAAAATCTTGAATTTATGGGGCGGGAGGGTAGGAAAGCCTGTA
CCTGTCTGTTTTTTTCCTGATCCTTTTCCCTCATTCCTGAACTGCAGGAGACTGAGCCCCTTTGGGCTTTGGTGACCCCATCACTGGGGT
GTGTTTATTTGATGGTTGATTTTGCTGTACTGGGTACTTCCTTTCCCATTTTCTAATCATTTTTTAACACAAGCTGACTCTTCCCTTCCC

>In-frame_ENST00000530003_ENST00000254231_TCGA-55-8085-01A_RPS6KA1_chr1_26885428_+_LIN28A_chr1_26751794_length(amino acids)=530AA_start in transcript=26_stop in transcript=1618
MVTGLSPTMQTPADFPRVERDLVPCPRKDEGVLKEISITHHVKAGSEKADPSHFELLKVLGQGSFGKVFLVRKVTRPDSGHLYAMKVLKK
ATLKVRDRVRTKMERDILADVNHPFVVKLHYAFQTEGKLYLILDFLRGGDLFTRLSKEVMFTEEDVKFYLAELALGLDHLHSLGIIYRDL
KPENILLDEEGHIKLTDFGLSKEAIDHEKKAYSFCGTVEYMAPEVVNRQGHSHSADWWSYGVLMFEMLTGSLPFQGKDRKETMTLILKAK
LGMPQFLSTEAQSLLRALFKRNPANRLGSGPDGAEEIKRHVFYSTIDWNKLYRREIKPPFKPAVAQPDDTFYFDTEFTSRTPKDSPGIPP
SAGAHQLFRGFSFVATGLMEDDGKPRAPQAPLHSVVQSKLHMEGFRSLKEGEAVEFTFKKSAKGLESIRVTGPGGVFCIGSERRPKGKSM

--------------------------------------------------------------
>In-frame_ENST00000531382_ENST00000326279_TCGA-55-8085-01A_RPS6KA1_chr1_26885428_+_LIN28A_chr1_26751794_length(transcript)=4957nt_BP=1291nt
CCCCGGACTCTGCCTGCTCCTGCCATGGTGCCGTGGCCCTGCTGGGCGGATGGAGCAGGATCCCAAGCCGCCCCGTCTGCGGCTCTGGGC
CCTGATCCCCTGGCTTCCCAGGAAGCAGCGGCCCAGGATCAGCCAGACCTCTCTGCCTGTCCCTGGCCCTGGCTCTGGCCCCCAGCGGGA
CTCGGATGAGGGCGTCCTCAAGGAGATCTCCATCACGCACCACGTCAAGGCTGGCTCTGAGAAGGCTGATCCATCCCATTTCGAGCTCCT
CAAGGTTCTGGGCCAGGGATCCTTTGGCAAAGTCTTCCTGGTGCGGAAAGTCACCCGGCCTGACAGTGGGCACCTGTATGCTATGAAGGT
GCTGAAGAAGGCAACGCTGAAAGTACGTGACCGCGTCCGGACCAAGATGGAGAGAGACATCCTGGCTGATGTAAATCACCCATTCGTGGT
GAAGCTGCACTATGCCTTCCAGACCGAGGGCAAGCTCTATCTCATTCTGGACTTCCTGCGTGGTGGGGACCTCTTCACCCGGCTCTCAAA
AGAGGTGATGTTCACGGAGGAGGATGTGAAGTTTTACCTGGCTGAGCTGGCTCTGGGCCTGGATCACCTGCACAGCCTGGGTATCATTTA
CAGAGACCTCAAGCCTGAGAACATCCTTCTGGATGAGGAGGGCCACATCAAACTCACTGACTTTGGCCTGAGCAAAGAGGCCATTGACCA
CGAGAAGAAGGCCTATTCTTTCTGCGGGACAGTGGAGTACATGGCCCCTGAGGTCGTCAACCGCCAGGGCCACTCCCATAGTGCGGACTG
GTGGTCCTATGGGGTGTTGATGTTTGAGATGCTGACGGGCTCCCTGCCCTTCCAGGGGAAGGACCGGAAGGAGACCATGACACTGATTCT
GAAGGCGAAGCTAGGCATGCCCCAGTTTCTGAGCACTGAAGCCCAGAGCCTCTTGCGGGCCCTGTTCAAGCGGAATCCTGCCAACCGGCT
CGGCTCCGGCCCTGATGGGGCAGAGGAAATCAAGCGGCATGTCTTCTACTCCACCATTGACTGGAATAAGCTATACCGTCGTGAGATCAA
GCCACCCTTCAAGCCAGCAGTGGCTCAGCCTGATGACACCTTCTACTTTGACACCGAGTTCACGTCCCGCACACCCAAGGATTCCCCAGG
CATCCCCCCCAGCGCTGGGGCCCATCAGCTGTTCCGGGGCTTCAGCTTCGTGGCCACCGGCCTGATGGAAGACGACGGCAAGCCTCGTGC
CCCGCAGGCACCCCTGCACTCGGTGGTACAGAGTAAGCTGCACATGGAAGGGTTCCGGAGCTTGAAGGAGGGTGAGGCAGTGGAGTTCAC
CTTTAAGAAGTCAGCCAAGGGTCTGGAATCCATCCGTGTCACCGGACCTGGTGGAGTATTCTGTATTGGGAGTGAGAGGCGGCCAAAAGG
AAAGAGCATGCAGAAGCGCAGATCAAAAGGAGACAGGTGCTACAACTGTGGAGGTCTAGATCATCATGCCAAGGAATGCAAGCTGCCACC
CCAGCCCAAGAAGTGCCACTTCTGCCAGAGCATCAGCCATATGGTAGCCTCATGTCCGCTGAAGGCCCAGCAGGGCCCTAGTGCACAGGG
AAAGCCAACCTACTTTCGAGAGGAAGAAGAAGAAATCCACAGCCCTACCCTGCTCCCGGAGGCACAGAATTGAGCCACAATGGGTGGGGG
CTATTCTTTTGCTATCAGGAAGTTTTGAGGAGCAGGCAGAGTGGAGAAAGTGGGAATAGGGTGCATTGGGGCTAGTTGGCACTGCCATGT
ATCTCAGGCTTGGGTTCACACCATCACCCTTTCTTCCCTCTAGGTGGGGGGAAAGGGTGAGTCAAAGGAACTCCAACCATGCTCTGTCCA
AATGCAAGTGAGGGTTCTGGGGGCAACCAGGAGGGGGGAATCACCCTACAACCTGCATACTTTGAGTCTCCATCCCCAGAATTTCCAGCT
TTTGAAAGTGGCCTGGATAGGGAAGTTGTTTTCCTTTTAAAGAAGGATATATAATAATTCCCATGCCAGAGTGAAATGATTAAGTATAAG
ACCAGATTCATGGAGCCAAGCCACTACATTCTGTGGAAGGAGATCTCTCAGGAGTAAGCATTGTTTTTTTTTCACATCTTGTATCCTCAT
ACCCACTTTTGGGATAGGGTGCTGGCAGCTGTCCCAAGCAATGGGTAATGATGATGGCAAAAAGGGTGTTTGGGGGAACAGCTGCAGACC
TGCTGCTCTATGCTCACCCCCGCCCCATTCTGGGCCAATGTGATTTTATTTATTTGCTCCCTTGGATACTGCACCTTGGGTCCCACTTTC
TCCAGGATGCCAACTGCACTAGCTGTGTGCGAATGACGTATCTTGTGCATTTTAACTTTTTTTCCTTAATATAAATATTCTGGTTTTGTA
TTTTTGTATATTTTAATCTAAGGCCCTCATTTCCTGCACTGTGTTCTCAGGTACATGAGCAATCTCAGGGATAGCCAGCAGCAGCTCCAG
GTCTGCGCAGCAGGAATTACTTTTTGTTGTTTTTGCCACCGTGGAGAGCAACTATTTGGAGTGCACAGCCTATTGAACTACCTCATTTTT
GCCAATAAGAGCTGGCTTTTCTGCCATAGTGTCCTCTTGAAACCCCCTCTGCCTTGAAAATGTTTTATGGGAGACTAGGTTTTAACTGGG
TGGCCCCATGACTTGATTGCCTTCTACTGGAAGATTGGGAATTAGTCTAAACAGGAAATGGTGGTACACAGAGGCTAGGAGAGGCTGGGC
CCGGTGAAAAGGCCAGAGAGCAAGCCAAGATTAGGTGAGGGTTGTCTAATCCTATGGCACAGGACGTGCTTTACATCTCCAGATCTGTTC
TTCACCAGATTAGGTTAGGCCTACCATGTGCCACAGGGTGTGTGTGTGTTTGTAAAACTAGAGTTGCTAAGGATAAGTTTAAAGACCAAT
ACCCCTGTACTTAATCCTGTGCTGTCGAGGGATGGATATATGAAGTAAGGTGAGATCCTTAACCTTTCAAAATTTTCGGGTTCCAGGGAG
ACACACAAGCGAGGGTTTTGTGGTGCCTGGAGCCTGTGTCCTGCCCTGCTACAGTAGTGATTAATAGTGTCATGGTAGCTAAAGGAGAAA
AAGGGGGTTTCGTTTACACGCTGTGAGATCACCGCAAACCTACCTTACTGTGTTGAAACGGGACAAATGCAATAGAACGCATTGGGTGGT
GTGTGTCTGATCCTGGGTTCTTGTCTCCCCTAAATGCTGCCCCCCAAGTTACTGTATTTGTCTGGGCTTTGTAGGACTTCACTACGTTGA
TTGCTAGGTGGCCTAGTTTGTGTAAATATAATGTATTGGTCTTTCTCCGTGTTCTTTGGGGGTTTTGTTTACAAACTTCTTTTTGTATTG
AGAGAAAAATAGCCAAAGCATCTTTGACAGAAGGTTCTGCACCAGGCAAAAAGATCTGAAACATTAGTTTGGGGGGCCCTCTTCTTAAAG
TGGGGATCTTGAACCATCCTTTCTTTTGTATTCCCCTTCCCCTATTACCTATTAGACCAGATCTTCTGTCCTAAAAACTTGTCTTCTACC
CTGCCCTCTTTTCTGTTCACCCCCAAAAGAAAACTTACACACCCACACACATACACATTTCATGCTTGGAGTGTCTCCACAACTCTTAAA
TGATGTATGCAAAAATACTGAAGCTAGGAAAACCCTCCATCCCTTGTTCCCAACCTCCTAAGTCAAGACCATTACCATTTCTTTCTTTCT
TTTTTTTTTTTTTTTAAAATGGAGTCTCACTGTGTCACCCAGGCTGGAGTGCAGTGGCATGATCGGCTCACTGCAGCCTCTGCCTCTTGG
GTTCAAGTGATTCTCCTGCCTCAGCCTCCTGAGTAGCTGGGATTTCAGGCACCCGCCACACTCAGCTAATTTTTGTATTTTTAGTAGAGA
CGGGGTTTCACCATGTTGTCCAGGCTGGTCTGGAACTCCTGACCTCAGGTGATCTGCCCACCTTGGCTTCCCAAAGTGCTGGGATTACAG
GCATGAGCCACCATGCTGGGCCAACCATTTCTTGGTGTATTCATGCCAAACACTTAAGACACTGCTGTAGCCCAGGCGCGGTGGCTCACA
CCTGTAATCCCAGCACTTTGGAAGGCTGAGGCGGGCGGATCACAAGGTCACGAGTTCAAAACTATCCTGGCCAACACAGTGAAACCCCGT
CTCTACTAAAATACAAAAAAATTAGCCGGGTGTGGTGGTGCATGCCTTTAGTCCTAGCTATTCAGGAGGCTGAGGCAGGGGAATCGCTTG
AACCCGAGAGGCAGAGGTTGCAGTGAGCTGAGATCGCACCACTGCACTCCAGCCTGGTTACAGAGCAAGACTCTGTCTCAAACAAAACAA
AACAAAACAAAAACACACTACTGTATTTTGGATGGATCAAACCTCCTTAATTTTAATTTCTAATCCTAAAGTAAAGAGATGCAATTGGGG
GCCTTCCATGTAGAAAGTGGGGTCAGGAGGCCAAGAAAGGGAATATGAATGTATATCCAAGTCACTCAGGAACTTTTATGCAGGTGCTAG
AAACTTTATGTCAAAGTGGCCACAAGATTGTTTAATAGGAGACGAACGAATGTAACTCCATGTTTACTGCTAAAAACCAAAGCTTTGTGT
AAAATCTTGAATTTATGGGGCGGGAGGGTAGGAAAGCCTGTACCTGTCTGTTTTTTTCCTGATCCTTTTCCCTCATTCCTGAACTGCAGG
AGACTGAGCCCCTTTGGGCTTTGGTGACCCCATCACTGGGGTGTGTTTATTTGATGGTTGATTTTGCTGTACTGGGTACTTCCTTTCCCA
TTTTCTAATCATTTTTTAACACAAGCTGACTCTTCCCTTCCCTTCTCCTTTCCCTGGGAAAATACAATGAATAAATAAAGACTTATTGGT

>In-frame_ENST00000531382_ENST00000326279_TCGA-55-8085-01A_RPS6KA1_chr1_26885428_+_LIN28A_chr1_26751794_length(amino acids)=559AA_start in transcript=13_stop in transcript=1692
MLLPWCRGPAGRMEQDPKPPRLRLWALIPWLPRKQRPRISQTSLPVPGPGSGPQRDSDEGVLKEISITHHVKAGSEKADPSHFELLKVLG
QGSFGKVFLVRKVTRPDSGHLYAMKVLKKATLKVRDRVRTKMERDILADVNHPFVVKLHYAFQTEGKLYLILDFLRGGDLFTRLSKEVMF
TEEDVKFYLAELALGLDHLHSLGIIYRDLKPENILLDEEGHIKLTDFGLSKEAIDHEKKAYSFCGTVEYMAPEVVNRQGHSHSADWWSYG
VLMFEMLTGSLPFQGKDRKETMTLILKAKLGMPQFLSTEAQSLLRALFKRNPANRLGSGPDGAEEIKRHVFYSTIDWNKLYRREIKPPFK
PAVAQPDDTFYFDTEFTSRTPKDSPGIPPSAGAHQLFRGFSFVATGLMEDDGKPRAPQAPLHSVVQSKLHMEGFRSLKEGEAVEFTFKKS
AKGLESIRVTGPGGVFCIGSERRPKGKSMQKRRSKGDRCYNCGGLDHHAKECKLPPQPKKCHFCQSISHMVASCPLKAQQGPSAQGKPTY

--------------------------------------------------------------
>In-frame_ENST00000531382_ENST00000254231_TCGA-55-8085-01A_RPS6KA1_chr1_26885428_+_LIN28A_chr1_26751794_length(transcript)=4446nt_BP=1291nt
CCCCGGACTCTGCCTGCTCCTGCCATGGTGCCGTGGCCCTGCTGGGCGGATGGAGCAGGATCCCAAGCCGCCCCGTCTGCGGCTCTGGGC
CCTGATCCCCTGGCTTCCCAGGAAGCAGCGGCCCAGGATCAGCCAGACCTCTCTGCCTGTCCCTGGCCCTGGCTCTGGCCCCCAGCGGGA
CTCGGATGAGGGCGTCCTCAAGGAGATCTCCATCACGCACCACGTCAAGGCTGGCTCTGAGAAGGCTGATCCATCCCATTTCGAGCTCCT
CAAGGTTCTGGGCCAGGGATCCTTTGGCAAAGTCTTCCTGGTGCGGAAAGTCACCCGGCCTGACAGTGGGCACCTGTATGCTATGAAGGT
GCTGAAGAAGGCAACGCTGAAAGTACGTGACCGCGTCCGGACCAAGATGGAGAGAGACATCCTGGCTGATGTAAATCACCCATTCGTGGT
GAAGCTGCACTATGCCTTCCAGACCGAGGGCAAGCTCTATCTCATTCTGGACTTCCTGCGTGGTGGGGACCTCTTCACCCGGCTCTCAAA
AGAGGTGATGTTCACGGAGGAGGATGTGAAGTTTTACCTGGCTGAGCTGGCTCTGGGCCTGGATCACCTGCACAGCCTGGGTATCATTTA
CAGAGACCTCAAGCCTGAGAACATCCTTCTGGATGAGGAGGGCCACATCAAACTCACTGACTTTGGCCTGAGCAAAGAGGCCATTGACCA
CGAGAAGAAGGCCTATTCTTTCTGCGGGACAGTGGAGTACATGGCCCCTGAGGTCGTCAACCGCCAGGGCCACTCCCATAGTGCGGACTG
GTGGTCCTATGGGGTGTTGATGTTTGAGATGCTGACGGGCTCCCTGCCCTTCCAGGGGAAGGACCGGAAGGAGACCATGACACTGATTCT
GAAGGCGAAGCTAGGCATGCCCCAGTTTCTGAGCACTGAAGCCCAGAGCCTCTTGCGGGCCCTGTTCAAGCGGAATCCTGCCAACCGGCT
CGGCTCCGGCCCTGATGGGGCAGAGGAAATCAAGCGGCATGTCTTCTACTCCACCATTGACTGGAATAAGCTATACCGTCGTGAGATCAA
GCCACCCTTCAAGCCAGCAGTGGCTCAGCCTGATGACACCTTCTACTTTGACACCGAGTTCACGTCCCGCACACCCAAGGATTCCCCAGG
CATCCCCCCCAGCGCTGGGGCCCATCAGCTGTTCCGGGGCTTCAGCTTCGTGGCCACCGGCCTGATGGAAGACGACGGCAAGCCTCGTGC
CCCGCAGGCACCCCTGCACTCGGTGGTACAGAGTAAGCTGCACATGGAAGGGTTCCGGAGCTTGAAGGAGGGTGAGGCAGTGGAGTTCAC
CTTTAAGAAGTCAGCCAAGGGTCTGGAATCCATCCGTGTCACCGGACCTGGTGGAGTATTCTGTATTGGGAGTGAGAGGCGGCCAAAAGG
AAAGAGCATGCAGAAGCGCAGATCAAAAGGAGACAGGTGCTACAACTGTGGAGGTCTAGATCATCATGCCAAGGAATGCAAGCTGCCACC
CCAGCCCAAGAAGTGCCACTTCTGCCAGAGCATCAGCCATATGGTAGCCTCATGTCCGCTGAAGGCCCAGCAGGGCCCTAGTGCACAGGG
AAAGCCAACCTACTTTCGAGAGGAAGAAGAAGAAATCCACAGCCCTACCCTGCTCCCGGAGGCACAGAATTGAGCCACAATGGGTGGGGG
CTATTCTTTTGCTATCAGGAAGTTTTGAGGAGCAGGCAGAGTGGAGAAAGTGGGAATAGGGTGCATTGGGGCTAGTTGGCACTGCCATGT
ATCTCAGGCTTGGGTTCACACCATCACCCTTTCTTCCCTCTAGGTGGGGGGAAAGGGTGAGTCAAAGGAACTCCAACCATGCTCTGTCCA
AATGCAAGTGAGGGTTCTGGGGGCAACCAGGAGGGGGGAATCACCCTACAACCTGCATACTTTGAGTCTCCATCCCCAGAATTTCCAGCT
TTTGAAAGTGGCCTGGATAGGGAAGTTGTTTTCCTTTTAAAGAAGGATATATAATAATTCCCATGCCAGAGTGAAATGATTAAGTATAAG
ACCAGATTCATGGAGCCAAGCCACTACATTCTGTGGAAGGAGATCTCTCAGGAGTAAGCATTGTTTTTTTTTCACATCTTGTATCCTCAT
ACCCACTTTTGGGATAGGGTGCTGGCAGCTGTCCCAAGCAATGGGTAATGATGATGGCAAAAAGGGTGTTTGGGGGAACAGCTGCAGACC
TGCTGCTCTATGCTCACCCCCGCCCCATTCTGGGCCAATGTGATTTTATTTATTTGCTCCCTTGGATACTGCACCTTGGGTCCCACTTTC
TCCAGGATGCCAACTGCACTAGCTGTGTGCGAATGACGTATCTTGTGCATTTTAACTTTTTTTCCTTAATATAAATATTCTGGTTTTGTA
TTTTTGTATATTTTAATCTAAGGCCCTCATTTCCTGCACTGTGTTCTCAGGTACATGAGCAATCTCAGGGATAGCCAGCAGCAGCTCCAG
GTCTGCGCAGCAGGAATTACTTTTTGTTGTTTTTGCCACCGTGGAGAGCAACTATTTGGAGTGCACAGCCTATTGAACTACCTCATTTTT
GCCAATAAGAGCTGGCTTTTCTGCCATAGTGTCCTCTTGAAACCCCCTCTGCCTTGAAAATGTTTTATGGGAGACTAGGTTTTAACTGGG
TGGCCCCATGACTTGATTGCCTTCTACTGGAAGATTGGGAATTAGTCTAAACAGGAAATGGTGGTACACAGAGGCTAGGAGAGGCTGGGC
CCGGTGAAAAGGCCAGAGAGCAAGCCAAGATTAGGTGAGGGTTGTCTAATCCTATGGCACAGGACGTGCTTTACATCTCCAGATCTGTTC
TTCACCAGATTAGGTTAGGCCTACCATGTGCCACAGGGTGTGTGTGTGTTTGTAAAACTAGAGTTGCTAAGGATAAGTTTAAAGACCAAT
ACCCCTGTACTTAATCCTGTGCTGTCGAGGGATGGATATATGAAGTAAGGTGAGATCCTTAACCTTTCAAAATTTTCGGGTTCCAGGGAG
ACACACAAGCGAGGGTTTTGTGGTGCCTGGAGCCTGTGTCCTGCCCTGCTACAGTAGTGATTAATAGTGTCATGGTAGCTAAAGGAGAAA
AAGGGGGTTTCGTTTACACGCTGTGAGATCACCGCAAACCTACCTTACTGTGTTGAAACGGGACAAATGCAATAGAACGCATTGGGTGGT
GTGTGTCTGATCCTGGGTTCTTGTCTCCCCTAAATGCTGCCCCCCAAGTTACTGTATTTGTCTGGGCTTTGTAGGACTTCACTACGTTGA
TTGCTAGGTGGCCTAGTTTGTGTAAATATAATGTATTGGTCTTTCTCCGTGTTCTTTGGGGGTTTTGTTTACAAACTTCTTTTTGTATTG
AGAGAAAAATAGCCAAAGCATCTTTGACAGAAGGTTCTGCACCAGGCAAAAAGATCTGAAACATTAGTTTGGGGGGCCCTCTTCTTAAAG
TGGGGATCTTGAACCATCCTTTCTTTTGTATTCCCCTTCCCCTATTACCTATTAGACCAGATCTTCTGTCCTAAAAACTTGTCTTCTACC
CTGCCCTCTTTTCTGTTCACCCCCAAAAGAAAACTTACACACCCACACACATACACATTTCATGCTTGGAGTGTCTCCACAACTCTTAAA
TGATGTATGCAAAAATACTGAAGCTAGGAAAACCCTCCATCCCTTGTTCCCAACCTCCTAAGTCAAGACCATTACCATTTCTTTCTTTCT
TTTTTTTTTTTTTTTAAAATGGAGTCTCACTGTGTCACCCAGGCAGAGGTTGCAGTGAGCTGAGATCGCACCACTGCACTCCAGCCTGGT
TACAGAGCAAGACTCTGTCTCAAACAAAACAAAACAAAACAAAAACACACTACTGTATTTTGGATGGATCAAACCTCCTTAATTTTAATT
TCTAATCCTAAAGTAAAGAGATGCAATTGGGGGCCTTCCATGTAGAAAGTGGGGTCAGGAGGCCAAGAAAGGGAATATGAATGTATATCC
AAGTCACTCAGGAACTTTTATGCAGGTGCTAGAAACTTTATGTCAAAGTGGCCACAAGATTGTTTAATAGGAGACGAACGAATGTAACTC
CATGTTTACTGCTAAAAACCAAAGCTTTGTGTAAAATCTTGAATTTATGGGGCGGGAGGGTAGGAAAGCCTGTACCTGTCTGTTTTTTTC
CTGATCCTTTTCCCTCATTCCTGAACTGCAGGAGACTGAGCCCCTTTGGGCTTTGGTGACCCCATCACTGGGGTGTGTTTATTTGATGGT
TGATTTTGCTGTACTGGGTACTTCCTTTCCCATTTTCTAATCATTTTTTAACACAAGCTGACTCTTCCCTTCCCTTCTCCTTTCCCTGGG

>In-frame_ENST00000531382_ENST00000254231_TCGA-55-8085-01A_RPS6KA1_chr1_26885428_+_LIN28A_chr1_26751794_length(amino acids)=559AA_start in transcript=13_stop in transcript=1692
MLLPWCRGPAGRMEQDPKPPRLRLWALIPWLPRKQRPRISQTSLPVPGPGSGPQRDSDEGVLKEISITHHVKAGSEKADPSHFELLKVLG
QGSFGKVFLVRKVTRPDSGHLYAMKVLKKATLKVRDRVRTKMERDILADVNHPFVVKLHYAFQTEGKLYLILDFLRGGDLFTRLSKEVMF
TEEDVKFYLAELALGLDHLHSLGIIYRDLKPENILLDEEGHIKLTDFGLSKEAIDHEKKAYSFCGTVEYMAPEVVNRQGHSHSADWWSYG
VLMFEMLTGSLPFQGKDRKETMTLILKAKLGMPQFLSTEAQSLLRALFKRNPANRLGSGPDGAEEIKRHVFYSTIDWNKLYRREIKPPFK
PAVAQPDDTFYFDTEFTSRTPKDSPGIPPSAGAHQLFRGFSFVATGLMEDDGKPRAPQAPLHSVVQSKLHMEGFRSLKEGEAVEFTFKKS
AKGLESIRVTGPGGVFCIGSERRPKGKSMQKRRSKGDRCYNCGGLDHHAKECKLPPQPKKCHFCQSISHMVASCPLKAQQGPSAQGKPTY

--------------------------------------------------------------

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Fusion Gene PPI Analysis for RPS6KA1-LIN28A


check button Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in

ChiPPI page.


check button Protein-protein interactors with each fusion partner protein in wild-type (BIOGRID-3.4.160)
HgeneHgene's interactorsTgeneTgene's interactors


check button - Retained PPIs in in-frame fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenStill interaction with


check button - Lost PPIs in in-frame fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenInteraction lost with


check button - Retained PPIs, but lost function due to frame-shift fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenInteraction lost with


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Related Drugs for RPS6KA1-LIN28A


check button Drugs targeting genes involved in this fusion gene.
(DrugBank Version 5.1.8 2021-05-08)
PartnerGeneUniProtAccDrugBank IDDrug nameDrug activityDrug typeDrug status

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Related Diseases for RPS6KA1-LIN28A


check button Diseases associated with fusion partners.
(DisGeNet 4.0)
PartnerGeneDisease IDDisease name# pubmedsSource
HgeneRPS6KA1C0015923Fetal Alcohol Syndrome1CTD_human
HgeneRPS6KA1C0036920Sezary Syndrome1CTD_human
HgeneRPS6KA1C0814154Alcohol Related Neurodevelopmental Disorder1CTD_human
HgeneRPS6KA1C2985290Fetal Alcohol Spectrum Disorders1CTD_human
HgeneRPS6KA1C3146244Alcohol Related Birth Defect1CTD_human
HgeneRPS6KA1C3661483Partial Fetal Alcohol Syndrome1CTD_human
TgeneLIN28AC0006826Malignant Neoplasms1CTD_human
TgeneLIN28AC0007621Neoplastic Cell Transformation1CTD_human
TgeneLIN28AC0027626Neoplasm Invasiveness1CTD_human
TgeneLIN28AC0027651Neoplasms1CTD_human
TgeneLIN28AC0086692Benign Neoplasm1CTD_human