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Center for Computational Systems Medicine
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Fusion Gene Summary

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Fusion Gene ORF analysis

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Fusion Genomic Features

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Fusion Protein Features

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Fusion Gene Sequence

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Fusion Gene PPI analysis

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Related Drugs

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Related Diseases

Fusion gene:SKA2-MAPK1 (FusionGDB2 ID:81460)

Fusion Gene Summary for SKA2-MAPK1

check button Fusion gene summary
Fusion gene informationFusion gene name: SKA2-MAPK1
Fusion gene ID: 81460
HgeneTgene
Gene symbol

SKA2

MAPK1

Gene ID

348235

5594

Gene namespindle and kinetochore associated complex subunit 2mitogen-activated protein kinase 1
SynonymsFAM33AERK|ERK-2|ERK2|ERT1|MAPK2|P42MAPK|PRKM1|PRKM2|p38|p40|p41|p41mapk|p42-MAPK
Cytomap

17q22

22q11.22

Type of geneprotein-codingprotein-coding
Descriptionspindle and kinetochore-associated protein 2family with sequence similarity 33, member Aspindle and KT (kinetochore) associated 2mitogen-activated protein kinase 1MAP kinase 1MAP kinase 2MAP kinase isoform p42MAPK 2extracellular signal-regulated kinase 2mitogen-activated protein kinase 2protein tyrosine kinase ERK2
Modification date2020031320200327
UniProtAcc.

Q8NDC0

Ensembl transtripts involved in fusion geneENST00000330137, ENST00000580541, 
ENST00000578105, ENST00000583927, 
ENST00000581068, ENST00000437036, 
ENST00000583380, 		ENST00000215832, 
ENST00000398822, ENST00000544786, 
ENST00000491588, 
Fusion gene scores* DoF score13 X 10 X 5=65015 X 8 X 9=1080
# samples 1416
** MAII scorelog2(14/650*10)=-2.21501289097085
possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs).
DoF>8 and MAII<0		log2(16/1080*10)=-2.75488750216347
possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs).
DoF>8 and MAII<0
Context

PubMed: SKA2 [Title/Abstract] AND MAPK1 [Title/Abstract] AND fusion [Title/Abstract]

Most frequent breakpointSKA2(57232491)-MAPK1(22162135), # samples:1
Anticipated loss of major functional domain due to fusion event.SKA2-MAPK1 seems lost the major protein functional domain in Hgene partner, which is a essential gene due to the frame-shifted ORF.
SKA2-MAPK1 seems lost the major protein functional domain in Tgene partner, which is a kinase due to the frame-shifted ORF.
SKA2-MAPK1 seems lost the major protein functional domain in Tgene partner, which is a CGC due to the frame-shifted ORF.
SKA2-MAPK1 seems lost the major protein functional domain in Tgene partner, which is a IUPHAR drug target due to the frame-shifted ORF.
* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

check button Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez
PartnerGeneGO IDGO termPubMed ID
HgeneSKA2

GO:0031110

regulation of microtubule polymerization or depolymerization

19289083

TgeneMAPK1

GO:0006468

protein phosphorylation

23184662

TgeneMAPK1

GO:0010800

positive regulation of peptidyl-threonine phosphorylation

16314496

TgeneMAPK1

GO:0018105

peptidyl-serine phosphorylation

15850461

TgeneMAPK1

GO:0034198

cellular response to amino acid starvation

11096076

TgeneMAPK1

GO:0038127

ERBB signaling pathway

15133037

TgeneMAPK1

GO:0051403

stress-activated MAPK cascade

11096076

TgeneMAPK1

GO:0070371

ERK1 and ERK2 cascade

16314496

TgeneMAPK1

GO:0070849

response to epidermal growth factor

18794356


check buttonFusion gene breakpoints across SKA2 (5'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

check buttonFusion gene breakpoints across MAPK1 (3'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

check button Fusion gene information from two resources (ChiTars 5.0 and ChimerDB 4.0)
* All genome coordinats were lifted-over on hg19.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.
SourceDiseaseSampleHgeneHchrHbpHstrandTgeneTchrTbpTstrand
ChimerDB4OVTCGA-04-1365SKA2chr17

57232491

-MAPK1chr22

22162135

-


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Fusion Gene ORF analysis for SKA2-MAPK1

check button Open reading frame (ORF) analsis of fusion genes based on Ensembl gene isoform structure.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.
ORFHenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrand
In-frameENST00000330137ENST00000215832SKA2chr17

57232491

-MAPK1chr22

22162135

-
Frame-shiftENST00000330137ENST00000398822SKA2chr17

57232491

-MAPK1chr22

22162135

-
Frame-shiftENST00000330137ENST00000544786SKA2chr17

57232491

-MAPK1chr22

22162135

-
5CDS-intronENST00000330137ENST00000491588SKA2chr17

57232491

-MAPK1chr22

22162135

-
intron-3CDSENST00000580541ENST00000215832SKA2chr17

57232491

-MAPK1chr22

22162135

-
intron-3CDSENST00000580541ENST00000398822SKA2chr17

57232491

-MAPK1chr22

22162135

-
intron-3CDSENST00000580541ENST00000544786SKA2chr17

57232491

-MAPK1chr22

22162135

-
intron-intronENST00000580541ENST00000491588SKA2chr17

57232491

-MAPK1chr22

22162135

-
In-frameENST00000578105ENST00000215832SKA2chr17

57232491

-MAPK1chr22

22162135

-
Frame-shiftENST00000578105ENST00000398822SKA2chr17

57232491

-MAPK1chr22

22162135

-
Frame-shiftENST00000578105ENST00000544786SKA2chr17

57232491

-MAPK1chr22

22162135

-
5CDS-intronENST00000578105ENST00000491588SKA2chr17

57232491

-MAPK1chr22

22162135

-
5UTR-3CDSENST00000583927ENST00000215832SKA2chr17

57232491

-MAPK1chr22

22162135

-
5UTR-3CDSENST00000583927ENST00000398822SKA2chr17

57232491

-MAPK1chr22

22162135

-
5UTR-3CDSENST00000583927ENST00000544786SKA2chr17

57232491

-MAPK1chr22

22162135

-
5UTR-intronENST00000583927ENST00000491588SKA2chr17

57232491

-MAPK1chr22

22162135

-
In-frameENST00000581068ENST00000215832SKA2chr17

57232491

-MAPK1chr22

22162135

-
Frame-shiftENST00000581068ENST00000398822SKA2chr17

57232491

-MAPK1chr22

22162135

-
Frame-shiftENST00000581068ENST00000544786SKA2chr17

57232491

-MAPK1chr22

22162135

-
5CDS-intronENST00000581068ENST00000491588SKA2chr17

57232491

-MAPK1chr22

22162135

-
intron-3CDSENST00000437036ENST00000215832SKA2chr17

57232491

-MAPK1chr22

22162135

-
intron-3CDSENST00000437036ENST00000398822SKA2chr17

57232491

-MAPK1chr22

22162135

-
intron-3CDSENST00000437036ENST00000544786SKA2chr17

57232491

-MAPK1chr22

22162135

-
intron-intronENST00000437036ENST00000491588SKA2chr17

57232491

-MAPK1chr22

22162135

-
In-frameENST00000583380ENST00000215832SKA2chr17

57232491

-MAPK1chr22

22162135

-
Frame-shiftENST00000583380ENST00000398822SKA2chr17

57232491

-MAPK1chr22

22162135

-
Frame-shiftENST00000583380ENST00000544786SKA2chr17

57232491

-MAPK1chr22

22162135

-
5CDS-intronENST00000583380ENST00000491588SKA2chr17

57232491

-MAPK1chr22

22162135

-

check buttonORFfinder result based on the fusion transcript sequence of in-frame fusion genes.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandSeq length
(transcript)		BP loci
(transcript)		Predicted start
(transcript)		Predicted stop
(transcript)		Seq length
(amino acids)
ENST00000330137SKA2chr1757232491-ENST00000215832MAPK1chr2222162135-108531392241102292
ENST00000578105SKA2chr1757232491-ENST00000215832MAPK1chr2222162135-10768541391017292
ENST00000581068SKA2chr1757232491-ENST00000215832MAPK1chr2222162135-108321182031081292
ENST00000583380SKA2chr1757232491-ENST00000215832MAPK1chr2222162135-10758441291007292

check buttonDeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandNo-coding scoreCoding score
ENST00000330137ENST00000215832SKA2chr1757232491-MAPK1chr2222162135-0.0010386230.9989613
ENST00000578105ENST00000215832SKA2chr1757232491-MAPK1chr2222162135-0.0010287210.99897134
ENST00000581068ENST00000215832SKA2chr1757232491-MAPK1chr2222162135-0.001037040.998963
ENST00000583380ENST00000215832SKA2chr1757232491-MAPK1chr2222162135-0.0010345280.9989655

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Fusion Genomic Features for SKA2-MAPK1


check buttonFusionAI prediction of the potential fusion gene breakpoint based on the pre-mature RNA sequence context (+/- 5kb of individual partner genes, total 20kb length sequence). FusionAI is a fusion gene breakpoint classifier based on convolutional neural network by comparing the fusion positive and negative sequence context of ~ 20K fusion gene data. From here, we can have the relative potentency of the 20K genomic sequence how individual sequnce will be likely used as the gene fusion breakpoints.
HgeneHchrHbpHstrandTgeneTchrTbpTstrand1-pp (fusion gene breakpoint)

check buttonDistribution of 44 human genomic features loci across 20kb length fusion breakpoint regions. We integrated a total of 44 different types of human genomic feature loci information across five big categories including virus integration sites, repeats, structural variants, chromatin states, and gene expression regulation. More details are in help page.

check buttonDistribution of 44 human genomic features loci across 20kb length fusion breakpoint regions that are ovelapped with the top 1% feature importance score regions. More details are in help page.

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Fusion Protein Features for SKA2-MAPK1


check button Go to

FGviewer for the breakpoints of chr17:57232491-chr22:22162135

.
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
FGviewer

check buttonMain function of each fusion partner protein. (from UniProt)
HgeneTgene
.MAPK1

Q8NDC0

FUNCTION: Might normally function as a transcriptional repressor. EWS-fusion-proteins (EFPS) may play a role in the tumorigenic process. They may disturb gene expression by mimicking, or interfering with the normal function of CTD-POLII within the transcription initiation complex. They may also contribute to an aberrant activation of the fusion protein target genes.

check buttonRetention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at

download page


* Minus value of BPloci means that the break pointn is located before the CDS.
- In-frame and retained protein feature among the 13 regional features.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note
TgeneMAPK1chr17:57232491chr22:22162135ENST0000021583209259_27739.6666666666666643486.3333333333335DNA binding.
TgeneMAPK1chr17:57232491chr22:22162135ENST0000039882208259_27739.666666666666664361.0DNA binding.
TgeneMAPK1chr17:57232491chr22:22162135ENST0000054478607259_27739.666666666666664317.0DNA binding.
TgeneMAPK1chr17:57232491chr22:22162135ENST0000021583209185_18739.6666666666666643486.3333333333335MotifTXY
TgeneMAPK1chr17:57232491chr22:22162135ENST0000021583209318_32239.6666666666666643486.3333333333335MotifCytoplasmic retention motif
TgeneMAPK1chr17:57232491chr22:22162135ENST0000021583209327_33339.6666666666666643486.3333333333335MotifNuclear translocation motif
TgeneMAPK1chr17:57232491chr22:22162135ENST0000039882208185_18739.666666666666664361.0MotifTXY
TgeneMAPK1chr17:57232491chr22:22162135ENST0000039882208318_32239.666666666666664361.0MotifCytoplasmic retention motif
TgeneMAPK1chr17:57232491chr22:22162135ENST0000039882208327_33339.666666666666664361.0MotifNuclear translocation motif
TgeneMAPK1chr17:57232491chr22:22162135ENST0000054478607185_18739.666666666666664317.0MotifTXY
TgeneMAPK1chr17:57232491chr22:22162135ENST0000054478607318_32239.666666666666664317.0MotifCytoplasmic retention motif
TgeneMAPK1chr17:57232491chr22:22162135ENST0000054478607327_33339.666666666666664317.0MotifNuclear translocation motif
TgeneMAPK1chr17:57232491chr22:22162135ENST0000021583209105_10839.6666666666666643486.3333333333335RegionNote=Inhibitor-binding
TgeneMAPK1chr17:57232491chr22:22162135ENST0000021583209153_15439.6666666666666643486.3333333333335RegionNote=Inhibitor-binding
TgeneMAPK1chr17:57232491chr22:22162135ENST0000039882208105_10839.666666666666664361.0RegionNote=Inhibitor-binding
TgeneMAPK1chr17:57232491chr22:22162135ENST0000039882208153_15439.666666666666664361.0RegionNote=Inhibitor-binding
TgeneMAPK1chr17:57232491chr22:22162135ENST0000054478607105_10839.666666666666664317.0RegionNote=Inhibitor-binding
TgeneMAPK1chr17:57232491chr22:22162135ENST0000054478607153_15439.666666666666664317.0RegionNote=Inhibitor-binding

- In-frame and not-retained protein feature among the 13 regional features.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note
TgeneMAPK1chr17:57232491chr22:22162135ENST00000215832092_939.6666666666666643486.3333333333335Compositional biasNote=Poly-Ala
TgeneMAPK1chr17:57232491chr22:22162135ENST00000398822082_939.666666666666664361.0Compositional biasNote=Poly-Ala
TgeneMAPK1chr17:57232491chr22:22162135ENST00000544786072_939.666666666666664317.0Compositional biasNote=Poly-Ala
TgeneMAPK1chr17:57232491chr22:22162135ENST000002158320925_31339.6666666666666643486.3333333333335DomainProtein kinase
TgeneMAPK1chr17:57232491chr22:22162135ENST000003988220825_31339.666666666666664361.0DomainProtein kinase
TgeneMAPK1chr17:57232491chr22:22162135ENST000005447860725_31339.666666666666664317.0DomainProtein kinase
TgeneMAPK1chr17:57232491chr22:22162135ENST000002158320931_3939.6666666666666643486.3333333333335Nucleotide bindingATP
TgeneMAPK1chr17:57232491chr22:22162135ENST000003988220831_3939.666666666666664361.0Nucleotide bindingATP
TgeneMAPK1chr17:57232491chr22:22162135ENST000005447860731_3939.666666666666664317.0Nucleotide bindingATP


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Fusion Gene Sequence for SKA2-MAPK1


check button For in-frame fusion transcripts, we provide the fusion transcript sequences and fusion amino acid sequences. To have fusion amino acid sequence, we ran ORFfinder and chose the longest ORF among the all predicted ones.
>In-frame_ENST00000330137_ENST00000215832_TCGA-04-1365_SKA2_chr17_57232491_-_MAPK1_chr22_22162135_length(transcript)=10853nt_BP=139nt
AATGAGTGCGAGATGTTGAGTGACAGCTGTCCAATGGAGGCCCTCCGTGCTAGTACGGCTTGCGGCTTAAGCGCCGCCGCGGTCTGCGGA
ATGTCAACTATTCAACATGGAGGCGGAGGTCGATAAGCTGGAACTGATGCTCTGCTTATGATAATGTCAACAAAGTTCGAGTAGCTATCA
AGAAAATCAGCCCCTTTGAGCACCAGACCTACTGCCAGAGAACCCTGAGGGAGATAAAAATCTTACTGCGCTTCAGACATGAGAACATCA
TTGGAATCAATGACATTATTCGAGCACCAACCATCGAGCAAATGAAAGATGTATATATAGTACAGGACCTCATGGAAACAGATCTTTACA
AGCTCTTGAAGACACAACACCTCAGCAATGACCATATCTGCTATTTTCTCTACCAGATCCTCAGAGGGTTAAAATATATCCATTCAGCTA
ACGTTCTGCACCGTGACCTCAAGCCTTCCAACCTGCTGCTCAACACCACCTGTGATCTCAAGATCTGTGACTTTGGCCTGGCCCGTGTTG
CAGATCCAGACCATGATCACACAGGGTTCCTGACAGAATATGTGGCCACACGTTGGTACAGGGCTCCAGAAATTATGTTGAATTCCAAGG
GCTACACCAAGTCCATTGATATTTGGTCTGTAGGCTGCATTCTGGCAGAAATGCTTTCTAACAGGCCCATCTTTCCAGGGAAGCATTATC
TTGACCAGCTGAACCACATTTTGGGTATTCTTGGATCCCCATCACAAGAAGACCTGAATTGTATAATAAATTTAAAAGCTAGGAACTATT
TGCTTTCTCTTCCACACAAAAATAAGGTGCCATGGAACAGGCTGTTCCCAAATGCTGACTCCAAAGCTCTGGACTTATTGGACAAAATGT
TGACATTCAACCCACACAAGAGGATTGAAGTAGAACAGGCTCTGGCCCACCCATATCTGGAGCAGTATTACGACCCGAGTGACGAGCCCA
TCGCCGAAGCACCATTCAAGTTCGACATGGAATTGGATGACTTGCCTAAGGAAAAGCTCAAAGAACTAATTTTTGAAGAGACTGCTAGAT
TCCAGCCAGGATACAGATCTTAAATTTGTCAGGACAAGGGCTCAGAGGACTGGACGTGCTCAGACATCGGTGTTCTTCTTCCCAGTTCTT
GACCCCTGGTCCTGTCTCCAGCCCGTCTTGGCTTATCCACTTTGACTCCTTTGAGCCGTTTGGAGGGGCGGTTTCTGGTAGTTGTGGCTT
TTATGCTTTCAAAGAATTTCTTCAGTCCAGAGAATTCCTCCTGGCAGCCCTGTGTGTGTCACCCATTGGTGACCTGCGGCAGTATGTACT
TCAGTGCACCTACTGCTTACTGTTGCTTTAGTCACTAATTGCTTTCTGGTTTGAAAGATGCAGTGGTTCCTCCCTCTCCTGAATCCTTTT
CTACATGATGCCCTGCTGACCATGCAGCCGCACCAGAGAGAGATTCTTCCCCAATTGGCTCTAGTCACTGGCATCTCACTTTATGATAGG
GAAGGCTACTACCTAGGGCACTTTAAGTCAGTGACAGCCCCTTATTTGCACTTCACCTTTTGACCATAACTGTTTCCCCAGAGCAGGAGC
TTGTGGAAATACCTTGGCTGATGTTGCAGCCTGCAGCAAGTGCTTCCGTCTCCGGAATCCTTGGGGAGCACTTGTCCACGTCTTTTCTCA
TATCATGGTAGTCACTAACATATATAAGGTATGTGCTATTGGCCCAGCTTTTAGAAAATGCAGTCATTTTTCTAAATAAAAAGGAAGTAC
TGCACCCAGCAGTGTCACTCTGTAGTTACTGTGGTCACTTGTACCATATAGAGGTGTAACACTTGTCAAGAAGCGTTATGTGCAGTACTT
AATGTTTGTAAGACTTACAAAAAAAGATTTAAAGTGGCAGCTTCACTCGACATTTGGTGAGAGAAGTACAAAGGTTGCAGTGCTGAGCTG
TGGGCGGTTTCTGGGGATGTCCCAGGGTGGAACTCCACATGCTGGTGCATATACGCCCTTGAGCTACTTCAAATGTGGGTGTTTCAGTAA
CCACGTTCCATGCCTGAGGATTTAGCAGAGAGGAACACTGCGTCTTTAAATGAGAAAGTATACAATTCTTTTTCCTTCTACAGCATGTCA
GCATCTCAAGTTCATTTTTCAACCTACAGTATAACAATTTGTAATAAAGCCTCCAGGAGCTCATGACGTGAAGCACTGTTCTGTCCTCAA
GTACTCAAATATTTCTGATACTGCTGAGTCAGACTGTCAGAAAAAGCTAGCACTAACTCGTGTTTGGAGCTCTATCCATATTTTACTGAT
CTCTTTAAGTATTTGTTCCTGCCACTGTGTACTGTGGAGTTGACTCGGTGTTCTGTCCCAGTGCGGTGCCTCCTCTTGACTTCCCCACTG
CTCTCTGTGGTGAGAAATTTGCCTTGTTCAATAATTACTGTACCCTCGCATGACTGTTACAGCTTTCTGTGCAGAGATGACTGTCCAAGT
GCCACATGCCTACGATTGAAATGAAAACTCTATTGTTACCTCTGAGTTGTGTTCCACGGAAAATGCTATCCAGCAGATCATTTAGGAAAA
ATAATTCTATTTTTAGCTTTTCATTTCTCAGCTGTCCTTTTTTCTTGTTTGATTTTTGACAGCAATGGAGAATGGGTTATATAAAGACTG
CCTGCTAATATGAACAGAAATGCATTTGTAATTCATGAAAATAAATGTACATCTTCTATCTTCACATTCATGTTAAGATTCAGTGTTGCT
TTCCTCTGGATCAGCGTGTCTGAATGGACAGTCAGGTTCAGGTTGTGCTGAACACAGAAATGCTCACAGGCCTCACTTTGCCGCCCAGGC
ACTGGCCCAGCACTTGGATTTACATAAGATGAGTTAGAAAGGTACTTCTGTAGGGTCCTTTTTACCTCTGCTCGGCAGAGAATCGATGCT
GTCATGTTCCTTTATTCACAATCTTAGGTCTCAAATATTCTGTCAAACCCTAACAAAGAAGCCCCGACATCTCAGGTTGGATTCCCTGGT
TCTCTCTAAAGAGGGCCTGCCCTTGTGCCCCAGAGGTGCTGCTGGGCACAGCCAAGAGTTGGGAAGGGCCGCCCCACAGTACGCAGTCCT
CACCACCCAGCCCAGGGTGCTCACGCTCACCACTCCTGTGGCTGAGGAAGGATAGCTGGCTCATCCTCGGAAAACAGACCCACATCTCTA
TTCTTGCCCTGAAATACGCGCTTTTCACTTGCGTGCTCAGAGCTGCCGTCTGAAGGTCCACACAGCATTGACGGGACACAGAAATGTGAC
TGTTACCGGATAACACTGATTAGTCAGTTTTCATTTATAAAAAAGCATTGACAGTTTTATTACTCTTGTTTCTTTTTAAATGGAAAGTTA
CTATTATAAGGTTAATTTGGAGTCCTCTTCTAAATAGAAAACCATATCCTTGGCTACTAACATCTGGAGACTGTGAGCTCCTTCCCATTC
CCCTTCCTGGTACTGTGGAGTCAGATTGGCATGAAACCACTAACTTCATTCTAGAATCATTGTAGCCATAAGTTGTGTGCTTTTTATTAA
TCATGCCAAACATAATGTAACTGGGCAGAGAATGGTCCTAACCAAGGTACCTATGAAAAGCGCTAGCTATCATGTGTAGTAGATGCATCA
TTTTGGCTCTTCTTACATTTGTAAAAATGTACAGATTAGGTCATCTTAATTCATATTAGTGACACGGAACAGCACCTCCACTATTTGTAT
GTTCAAATAAGCTTTCAGACTAATAGCTTTTTTGGTGTCTAAAATGTAAGCAAAAAATTCCTGCTGAAACATTCCAGTCCTTTCATTTAG
TATAAAAGAAATACTGAACAAGCCAGTGGGATGGAATTGAAAGAACTAATCATGAGGACTCTGTCCTGACACAGGTCCTCAAAGCTAGCA
GAGATACGCAGACATTGTGGCATCTGGGTAGAAGAATACTGTATTGTGTGTGCAGTGCACAGTGTGTGGTGTGTGCACACTCATTCCTTC
TGCTCTTGGGCACAGGCAGTGGGTGTAGAGGTAACCAGTAGCTTTGAGAAGCTACATGTAGCTCACCAGTGGTTTTCTCTAAGGAATCAC
AAAAGTAAACTACCCAACCACATGCCACGTAATATTTCAGCCATTCAGAGGAAACTGTTTTCTCTTTATTTGCTTATATGTTAATATGGT
TTTTAAATTGGTAACTTTTATATAGTATGGTAACAGTATGTTAATACACACATACATACGCACACATGCTTTGGGTCCTTCCATAATACT
TTTATATTTGTAAATCAATGTTTTGGAGCAATCCCAAGTTTAAGGGAAATATTTTTGTAAATGTAATGGTTTTGAAAATCTGAGCAATCC
TTTTGCTTATACATTTTTAAAGCATTTGTGCTTTAAAATTGTTATGCTGGTGTTTGAAACATGATACTCCTGTGGTGCAGATGAGAAGCT
ATAACAGTGAATATGTGGTTTCTCTTACGTCATCCACCTTGACATGATGGGTCAGAAACAAATGGAAATCCAGAGCAAGTCCTCCAGGGT
TGCACCAGGTTTACCTAAAGCTTGTTGCCTTTTCTTGTGCTGTTTATGCGTGTAGAGCACTCAAGAAAGTTCTGAAACTGCTTTGTATCT
GCTTTGTACTGTTGGTGCCTTCTTGGTATTGTACCCCAAAATTCTGCATAGATTATTTAGTATAATGGTAAGTTAAAAAATGTTAAAGGA
AGATTTTATTAAGAATCTGAATGTTTATTCATTATATTGTTACAATTTAACATTAACATTTATTTGTGGTATTTGTGATTTGGTTAATCT
GTATAAAAATTGTAAGTAGAAAGGTTTATATTTCATCTTAATTCTTTTGATGTTGTAAACGTACTTTTTAAAAGATGGATTATTTGAATG
TTTATGGCACCTGACTTGTAAAAAAAAAAAACTACAAAAAAATCCTTAGAATCATTAAATTGTGTCCCTGTATTACCAAAATAACACAGC
ACCGTGCATGTATAGTTTAATTGCAGTTTCATCTGTGAAAACGTGAAATTGTCTAGTCCTTCGTTATGTTCCCCAGATGTCTTCCAGATT
TGCTCTGCATGTGGTAACTTGTGTTAGGGCTGTGAGCTGTTCCTCGAGTTGAATGGGGATGTCAGTGCTCCTAGGGTTCTCCAGGTGGTT
CTTCAGACCTTCACCTGTGGGGGGGGGGGTAGGCGGTGCCCACGCCCATCTCCTCATCCTCCTGAACTTCTGCAACCCCACTGCTGGGCA
GACATCCTGGGCAACCCCTTTTTTCAGAGCAAGAAGTCATAAAGATAGGATTTCTTGGACATTTGGTTCTTATCAATATTGGGCATTATG
TAATGACTTATTTACAAAACAAAGATACTGGAAAATGTTTTGGATGTGGTGTTATGGAAAGAGCACAGGCCTTGGACCCATCCAGCTGGG
TTCAGAACTACCCCCTGCTTATAACTGCGGCTGGCTGTGGGCCAGTCATTCTGCGTCTCTGCTTTCTTCCTCTGCTTCAGACTGTCAGCT
GTAAAGTGGAAGCAATATTACTTGCCTTGTATATGGTAAAGATTATAAAAATACATTTCAACTGTTCAGCATAGTACTTCAAAGCAAGTA
CTCAGTAAATAGCAAGTCTTTTTAAATGCTGCTTTATTTCACTAAATTTTGTTGTGAGGTGTCACTAAAATGCCTGCAAACAAACGTAAC
TGCTAATCTGAGAGGAAACCCTCTTACTAATCAGAGAAGAAACCCTCCTGTCAGAAACCTTCAGGGAAGTGAGCTGATCACACCTAAACT
GGGAGTTTGCAATGGGGTATTTGAAGCACTGTGGGAGTATTCCACTGGCCCTCTCCCTGAGAGACTTAACAGTCTTCCCTGTTGTCCAGA
TTCTGTATAAGGCAATCAGAATAATCATCTTCCTTGTTCAGCAGAGGAGCCTGGTCCCATTTTCCCCACTTTGTGATGGGCTTCTCTCAG
CGGTAGCTCAGCAGTTCCAGATGGCAGTTTGGACCAGCATCTAGGCTGGCCAGTTCGCTGTGTTTACTTAGAACCAACACGTTCAGAGCT
GGCCTGGACCATCTGAGGGGAACAGGAAACACCCCTAGGCTGTGGAAGCAAGTGCAGACCCCCACCCCCGGCCCTGAAGCCAAGGGGGCA
GGGTTTGGGAGTGGCCAAAGAGAAGCAGTGCAGGGATGGGTTTTCCTAGGGACAGGCTTAGCATTCCTGACTCTAGGAAGAAGGAGCAGT
GAGGCGGAGAAACAGTGGAGGGGATGGTGGCATTGGGCCCCATGGGGCCGAGATGGACACAGGGCTCGTTCTCTTGAGTCTGGTGCCAAG
GACAGCTGAAGACGACATCATTTTCAGGTGGAGAGGAGAGAGTGGAGGGAGATCATGCCCTGTGATGTGTCTTTTGCAGGTGAAGGTGGG
AGACAAGGTCTCTGCTGACGATGAGGCAGAGCCACCGTGAAAGTTGTAATAGGAGGACTGCCCGCCGCTGGAAGGGCCTGCAGTGACGCT
AGGACACCCTCTGCCTGCATGTCACGTTAGCTGGGCTGGGCGAAGTAGAAGACCAAGGGGAAGAGGTGCAGTGGGGAGACCAGGTGGGAT
GCAACCACAGGACCAGTGGAGGGGCTGTGGCACGTGGGCGGAGACTGAGTGGCTGGGCATGTGTTGTGGCTGAGCATGTGGTGTGGGCAG
TGGTCCTAGACCCCGCCATGTCCGGACAATGATATAGAGCGTCTCAGCATCGCCAGTCTAGACTGTCTATGGAGAGCAGAAAGTTGTCTG
GGGCTGCCTGGGGAACTGTGAGGCCAGCTATATCACCGTCGCTGATGGTGACATTACGGTGGTGGCAGGAGCAAGGAGAGAGGGAAGAAG
GACCCCGTCCAGCTTTAGTCACAAAATACCCAATGGAAGATGCCAGTGCCAATCCTGTGGGTTTCCTTGGGACTTCACACTGGCTTTCTT
ATCTGCTCCAGATCCATTCAGTAGTCACTGAGTTCCTGCCAAATACTTTGTAGCGCCAGAAGCCAGGAGCGGGGTCTGCAGCAGGGCAGT
CCCCATTTTCAGGAAATGCCTGGAGCTGCTGGTCCCTGAGAGAAAGGAAAACATCTTTCAGCCGTACGCAGGCCAAGAAGGCCAATGTCC
AGTAGCTTTGTGATTTTTTTTATATTTTTTTATTTATTTATTTTTGAGATAGAGTCTTGCTCTGTCGCTCAGGCTGGAGTGCAGTGGCGT
GATCTCCACTCACTGCAACTTCCGCCTCCTGGGGTCAAGCAGTTCTGCCTCAGCCTCCCGAGTAGCTGGGATTACAGGCACACGCCACCA
CACCCAGCTAATTTTTGTGTTTTTAGTAGAGACGGTTTCACCATGTCGGCCAGGCTGGTCTCAAACTCCTGACCTCAGATGATTCAGCCT
CCCAAAGTGCTGGGATTACAGGTGTGAGCCACTGCACCCAGCCTGTGATGTTTCTGTGGGGTTCCACAAATGTGTGTGTGTGTAAAAGCT
GATGATTACAGCAAGAATGTGAACAGTAGCAGTTTTCCATTTGAAGGCAAGTTTTGTCTTTATCTGGGTATCAGAAGGACCCTCTGGGCC
ATTGTCGCTTCCTGTACTCAGAGCCACCCTAGTACTACGGGCACACACAGAAAACAGCAGCCTGCGTACTTTCAAAGGAAAGGCATCTTT
AATCACCAATGCCTGGAAAAATTATTTTGTTTCCCTCTTCCTTCCGTCTTGTTTCCTAACTTCTTACCAAAGTTTAGAGTCTGAGTTTTT
CGTATAATAATGTCCCACATCCACACATCGGGCCTACAGATGCTCTCCCTTGAATCGACTGGAAACATGACACCGGTTCCATGCTCTGGA
ACTGTCACCTGTGATGTGCTGGGCTGTGTCCCAAGCACAGGAATCCCAGCAGTTTCAGCTCGATGCAGAACCACCATGCTCCAGACACAG
GCTTGGGAAAGACACGTCAAAATTAAAATACTAGGTAAGAGAAGCACCTGATTGGGTAGAAGTTGGAGAGGAATCCTGGAATTTTGTGGC
CAGAAGGAGCCACTGCCCCTTTTGTTTAGTAAGACTAGACAGTAACAGAAGCCAGTTGTCAGCTATGAAAGTGGTGGGTGAAGCAGGGGA
GGCTCCTCTATGGTGGGACCCTGGACAAGGGAAGCCGAATGTGTGAAGAAGGGGTGCGGGGGTGTGCGGTGCCCTAGGACACTAGGGCAA
AGGTTTCAAACCTGGAACAAGGCACTGGAGGAAGATCTGCTGCCAGTCAGCAGTGCGGGCCCTCGAGTTAGCAGTCCGTGCGCAGAGGGG
CCAGTTCTGAGACCAGTTTGGAGAGTCAGGCAGTGACCCATTGGCCATGTCATAATTCCTTCAGCCTGCCTCCTCTTTAATCCCAGAGAG
TGCTCTTTCTTCATACTTCCTTTAAAATACTAAATTGTTCCCATTCCATGGGGAGCTGGCTAGGCTTTACAGGCTAGGAAATGTAGGTTT
TCTGAGATGGAACCATCTACACAAGGAGGAGGAAGGCACTAAGACTACAGATGAGACCCATGACAGGGCTGAGCATTTGGAAGCCAACCC
TGGTTGCTTTTCAAGAATTGCTTTGTGGCTGGGTGCAGTGGTTCACACCTGTAATTGCAGCACTTTGGGAGGCTGAGGCAGGTGGATTGC
TTGAACCCAGGAGTTCGAGACCAGCCTGGGCAACATATGGGACACCCCACCGCCCCCGGCTCTGCAAAAAAATTAAAAATTAGCCAGGCG
TGGTGTCATGAGCCTGTGGTCTCAACTACTCAGGAGGCTGAGGCTGGAGGATCGCTTGAACCTGGGAGGTCAAGGCTCCAGTGAACCATA
ATTGTGCCACTGCACTGCAGCCTGGGCGACAATTTGTTTTCTAAATTGCTTTTGAAAGTCTACTGCATTACATATTCCAAAAAGCAGTGG
TTTTCAAATACTTTTATCACCGATATCCTTTTATGAAATGAAATCAGTAGAACTTTCTCTGCTCTGAATAAGCAAGGGTGGGAACCTGTC
TACCTCCCACAGATAGCATAATGTGCCTGCCATAGAGGAGCCAAAAAATGGTGATGGGAACTGAGAGGAGAGCAAATGTCACAAAAGACT
GAGCAATTGAGAAAACAAAACAAGACCACAGATGACTGTTAACGCCTCCACAGTGGACCAAGAAAGGACAGAGAGCTGGCAGCATGGGCA
TCACTGTCTGGTCGGCAGCAGGAAGGCCTCGCTAGGGAATTGAGTACAGTCATCTAACTAGTTTAAAAGTACAGGAAGGATGATTAAGGC
TATTGGAGAGGTCATACAAATAGGGGAGGGGCAGGCAATGGCTGATAAGACATGAATTTGTAAGGCGATGAGTATTGCAGTCAGCAAAAC
AAACGAGACTGCTCTCCCAACACATAACTCAGCAGGGAGGCCAGGCATTGGTTTAACCATTTAATATAAAGAAGTTAAAATTACAAATGC
GCTAAGTGCCTAAAGAAGAATAAGTGCAGGAATGAGAGCAGCATGGACTGCCACAGTTTTAGAATAAGCACTGTCACTGCTAGATTGGAA
ACAAAAATCCATAAATTTGGCCCGGTGTGGTGGCGGACGCCTGTAGTCCCAGCTACTTGGAGGCTGAGGCGTGAGAATCGCTTGAACCCG
GGAGGCGGAGGTTGCAGTGAGCCGAGATGGCGCCACTGCACTCCAGCCTGGGCGTCAGAGTGAGAACTCTGTATCAAAAAATAAAAATAA
AAAAAAGTCCATAAATCTGCAATGTCTCAGTTAAGAAAGAAAGACTGGGCCAATGCAGATTTCAAACCGGAGAAAGTCATACTGTCAGTG
AAGGCCGCCTGTGGCCGGAAGGCGCCAGGGGATTAGCACCCTGGACTCAGTGTTGCTGGGAAACAGGGCCCCAAGGCTGGGAGCACAGTG
TTTAAAGGGCATCTACCCAAGAAGGGAGCACAGGGCAAGGAGGAGCTGCAGGGGGGCTTGGCTGCCAAAGTGAATTCTGAGGAGAGAGCT
ATTGCTGCCTACGATATGCAGGCTGCACAGAACACAAGTGGAATCAGCAGGCAGGAGAGGCAGCTAACGACGCAGCCCGTTTCTTATTTC
TGTTTTCTCACAAGCGATGAAAGTGGAAAAGAGGGTGAGCAGGTGGCCCACACATGTGCCTCCAGTGCTGCGGCCCCTCCGGGGACCATC
AGCCAGGCCCCGGGGAGGGAGCCAGCCACAGTGTGTCCGGCTCTTCTCTGAAGGGAAGAGAGCCTTGAATAGACTGAAGCGAAGACGGTT
CTGCAAGGACAAGGCAGACCGAAGGCATTGGTTTTTTTTTTCAGATAAGGAGAATTAGACTCCCAAGTAGACACCAGAGTCACTGTTTGG
TTGGTGGGTGATAGTGGGGTCACAGTGGCTGCCTGTGCTCCCCCAGGGTGAGCGTGACTGTGCTAACCTGGGTGGGGCAGCATGCACACC
CCTCTGGCAGCCCTTTGTTGCTCGCTGATGACAAGTTTGGATGATCCCGCCAAACAGCTTGCTAAGATGTAGTCCCCAGTGTTGGAGGTG
GGGCCTGATGGGAGGTGCTACCCTTGTGAGATAAGGTTGTGTAAAAGCCTGTGGCACCTCCCCACACTGACGCTCTCACCCCTGCTCTGG
CCATGTGCCGCGCCTGCTCCCACTTCCCCTTCTGCCAGGAGTAAAAGCCCCCGAGACCTCCCAGAAGCCAAGCAGATGCTAGTGCCATGC

>In-frame_ENST00000330137_ENST00000215832_TCGA-04-1365_SKA2_chr17_57232491_-_MAPK1_chr22_22162135_length(amino acids)=292AA_start in transcript=224_stop in transcript=1102
MREIKILLRFRHENIIGINDIIRAPTIEQMKDVYIVQDLMETDLYKLLKTQHLSNDHICYFLYQILRGLKYIHSANVLHRDLKPSNLLLN
TTCDLKICDFGLARVADPDHDHTGFLTEYVATRWYRAPEIMLNSKGYTKSIDIWSVGCILAEMLSNRPIFPGKHYLDQLNHILGILGSPS
QEDLNCIINLKARNYLLSLPHKNKVPWNRLFPNADSKALDLLDKMLTFNPHKRIEVEQALAHPYLEQYYDPSDEPIAEAPFKFDMELDDL

--------------------------------------------------------------
>In-frame_ENST00000578105_ENST00000215832_TCGA-04-1365_SKA2_chr17_57232491_-_MAPK1_chr22_22162135_length(transcript)=10768nt_BP=54nt
GCGGAATGTCAACTATTCAACATGGAGGCGGAGGTCGATAAGCTGGAACTGATGCTCTGCTTATGATAATGTCAACAAAGTTCGAGTAGC
TATCAAGAAAATCAGCCCCTTTGAGCACCAGACCTACTGCCAGAGAACCCTGAGGGAGATAAAAATCTTACTGCGCTTCAGACATGAGAA
CATCATTGGAATCAATGACATTATTCGAGCACCAACCATCGAGCAAATGAAAGATGTATATATAGTACAGGACCTCATGGAAACAGATCT
TTACAAGCTCTTGAAGACACAACACCTCAGCAATGACCATATCTGCTATTTTCTCTACCAGATCCTCAGAGGGTTAAAATATATCCATTC
AGCTAACGTTCTGCACCGTGACCTCAAGCCTTCCAACCTGCTGCTCAACACCACCTGTGATCTCAAGATCTGTGACTTTGGCCTGGCCCG
TGTTGCAGATCCAGACCATGATCACACAGGGTTCCTGACAGAATATGTGGCCACACGTTGGTACAGGGCTCCAGAAATTATGTTGAATTC
CAAGGGCTACACCAAGTCCATTGATATTTGGTCTGTAGGCTGCATTCTGGCAGAAATGCTTTCTAACAGGCCCATCTTTCCAGGGAAGCA
TTATCTTGACCAGCTGAACCACATTTTGGGTATTCTTGGATCCCCATCACAAGAAGACCTGAATTGTATAATAAATTTAAAAGCTAGGAA
CTATTTGCTTTCTCTTCCACACAAAAATAAGGTGCCATGGAACAGGCTGTTCCCAAATGCTGACTCCAAAGCTCTGGACTTATTGGACAA
AATGTTGACATTCAACCCACACAAGAGGATTGAAGTAGAACAGGCTCTGGCCCACCCATATCTGGAGCAGTATTACGACCCGAGTGACGA
GCCCATCGCCGAAGCACCATTCAAGTTCGACATGGAATTGGATGACTTGCCTAAGGAAAAGCTCAAAGAACTAATTTTTGAAGAGACTGC
TAGATTCCAGCCAGGATACAGATCTTAAATTTGTCAGGACAAGGGCTCAGAGGACTGGACGTGCTCAGACATCGGTGTTCTTCTTCCCAG
TTCTTGACCCCTGGTCCTGTCTCCAGCCCGTCTTGGCTTATCCACTTTGACTCCTTTGAGCCGTTTGGAGGGGCGGTTTCTGGTAGTTGT
GGCTTTTATGCTTTCAAAGAATTTCTTCAGTCCAGAGAATTCCTCCTGGCAGCCCTGTGTGTGTCACCCATTGGTGACCTGCGGCAGTAT
GTACTTCAGTGCACCTACTGCTTACTGTTGCTTTAGTCACTAATTGCTTTCTGGTTTGAAAGATGCAGTGGTTCCTCCCTCTCCTGAATC
CTTTTCTACATGATGCCCTGCTGACCATGCAGCCGCACCAGAGAGAGATTCTTCCCCAATTGGCTCTAGTCACTGGCATCTCACTTTATG
ATAGGGAAGGCTACTACCTAGGGCACTTTAAGTCAGTGACAGCCCCTTATTTGCACTTCACCTTTTGACCATAACTGTTTCCCCAGAGCA
GGAGCTTGTGGAAATACCTTGGCTGATGTTGCAGCCTGCAGCAAGTGCTTCCGTCTCCGGAATCCTTGGGGAGCACTTGTCCACGTCTTT
TCTCATATCATGGTAGTCACTAACATATATAAGGTATGTGCTATTGGCCCAGCTTTTAGAAAATGCAGTCATTTTTCTAAATAAAAAGGA
AGTACTGCACCCAGCAGTGTCACTCTGTAGTTACTGTGGTCACTTGTACCATATAGAGGTGTAACACTTGTCAAGAAGCGTTATGTGCAG
TACTTAATGTTTGTAAGACTTACAAAAAAAGATTTAAAGTGGCAGCTTCACTCGACATTTGGTGAGAGAAGTACAAAGGTTGCAGTGCTG
AGCTGTGGGCGGTTTCTGGGGATGTCCCAGGGTGGAACTCCACATGCTGGTGCATATACGCCCTTGAGCTACTTCAAATGTGGGTGTTTC
AGTAACCACGTTCCATGCCTGAGGATTTAGCAGAGAGGAACACTGCGTCTTTAAATGAGAAAGTATACAATTCTTTTTCCTTCTACAGCA
TGTCAGCATCTCAAGTTCATTTTTCAACCTACAGTATAACAATTTGTAATAAAGCCTCCAGGAGCTCATGACGTGAAGCACTGTTCTGTC
CTCAAGTACTCAAATATTTCTGATACTGCTGAGTCAGACTGTCAGAAAAAGCTAGCACTAACTCGTGTTTGGAGCTCTATCCATATTTTA
CTGATCTCTTTAAGTATTTGTTCCTGCCACTGTGTACTGTGGAGTTGACTCGGTGTTCTGTCCCAGTGCGGTGCCTCCTCTTGACTTCCC
CACTGCTCTCTGTGGTGAGAAATTTGCCTTGTTCAATAATTACTGTACCCTCGCATGACTGTTACAGCTTTCTGTGCAGAGATGACTGTC
CAAGTGCCACATGCCTACGATTGAAATGAAAACTCTATTGTTACCTCTGAGTTGTGTTCCACGGAAAATGCTATCCAGCAGATCATTTAG
GAAAAATAATTCTATTTTTAGCTTTTCATTTCTCAGCTGTCCTTTTTTCTTGTTTGATTTTTGACAGCAATGGAGAATGGGTTATATAAA
GACTGCCTGCTAATATGAACAGAAATGCATTTGTAATTCATGAAAATAAATGTACATCTTCTATCTTCACATTCATGTTAAGATTCAGTG
TTGCTTTCCTCTGGATCAGCGTGTCTGAATGGACAGTCAGGTTCAGGTTGTGCTGAACACAGAAATGCTCACAGGCCTCACTTTGCCGCC
CAGGCACTGGCCCAGCACTTGGATTTACATAAGATGAGTTAGAAAGGTACTTCTGTAGGGTCCTTTTTACCTCTGCTCGGCAGAGAATCG
ATGCTGTCATGTTCCTTTATTCACAATCTTAGGTCTCAAATATTCTGTCAAACCCTAACAAAGAAGCCCCGACATCTCAGGTTGGATTCC
CTGGTTCTCTCTAAAGAGGGCCTGCCCTTGTGCCCCAGAGGTGCTGCTGGGCACAGCCAAGAGTTGGGAAGGGCCGCCCCACAGTACGCA
GTCCTCACCACCCAGCCCAGGGTGCTCACGCTCACCACTCCTGTGGCTGAGGAAGGATAGCTGGCTCATCCTCGGAAAACAGACCCACAT
CTCTATTCTTGCCCTGAAATACGCGCTTTTCACTTGCGTGCTCAGAGCTGCCGTCTGAAGGTCCACACAGCATTGACGGGACACAGAAAT
GTGACTGTTACCGGATAACACTGATTAGTCAGTTTTCATTTATAAAAAAGCATTGACAGTTTTATTACTCTTGTTTCTTTTTAAATGGAA
AGTTACTATTATAAGGTTAATTTGGAGTCCTCTTCTAAATAGAAAACCATATCCTTGGCTACTAACATCTGGAGACTGTGAGCTCCTTCC
CATTCCCCTTCCTGGTACTGTGGAGTCAGATTGGCATGAAACCACTAACTTCATTCTAGAATCATTGTAGCCATAAGTTGTGTGCTTTTT
ATTAATCATGCCAAACATAATGTAACTGGGCAGAGAATGGTCCTAACCAAGGTACCTATGAAAAGCGCTAGCTATCATGTGTAGTAGATG
CATCATTTTGGCTCTTCTTACATTTGTAAAAATGTACAGATTAGGTCATCTTAATTCATATTAGTGACACGGAACAGCACCTCCACTATT
TGTATGTTCAAATAAGCTTTCAGACTAATAGCTTTTTTGGTGTCTAAAATGTAAGCAAAAAATTCCTGCTGAAACATTCCAGTCCTTTCA
TTTAGTATAAAAGAAATACTGAACAAGCCAGTGGGATGGAATTGAAAGAACTAATCATGAGGACTCTGTCCTGACACAGGTCCTCAAAGC
TAGCAGAGATACGCAGACATTGTGGCATCTGGGTAGAAGAATACTGTATTGTGTGTGCAGTGCACAGTGTGTGGTGTGTGCACACTCATT
CCTTCTGCTCTTGGGCACAGGCAGTGGGTGTAGAGGTAACCAGTAGCTTTGAGAAGCTACATGTAGCTCACCAGTGGTTTTCTCTAAGGA
ATCACAAAAGTAAACTACCCAACCACATGCCACGTAATATTTCAGCCATTCAGAGGAAACTGTTTTCTCTTTATTTGCTTATATGTTAAT
ATGGTTTTTAAATTGGTAACTTTTATATAGTATGGTAACAGTATGTTAATACACACATACATACGCACACATGCTTTGGGTCCTTCCATA
ATACTTTTATATTTGTAAATCAATGTTTTGGAGCAATCCCAAGTTTAAGGGAAATATTTTTGTAAATGTAATGGTTTTGAAAATCTGAGC
AATCCTTTTGCTTATACATTTTTAAAGCATTTGTGCTTTAAAATTGTTATGCTGGTGTTTGAAACATGATACTCCTGTGGTGCAGATGAG
AAGCTATAACAGTGAATATGTGGTTTCTCTTACGTCATCCACCTTGACATGATGGGTCAGAAACAAATGGAAATCCAGAGCAAGTCCTCC
AGGGTTGCACCAGGTTTACCTAAAGCTTGTTGCCTTTTCTTGTGCTGTTTATGCGTGTAGAGCACTCAAGAAAGTTCTGAAACTGCTTTG
TATCTGCTTTGTACTGTTGGTGCCTTCTTGGTATTGTACCCCAAAATTCTGCATAGATTATTTAGTATAATGGTAAGTTAAAAAATGTTA
AAGGAAGATTTTATTAAGAATCTGAATGTTTATTCATTATATTGTTACAATTTAACATTAACATTTATTTGTGGTATTTGTGATTTGGTT
AATCTGTATAAAAATTGTAAGTAGAAAGGTTTATATTTCATCTTAATTCTTTTGATGTTGTAAACGTACTTTTTAAAAGATGGATTATTT
GAATGTTTATGGCACCTGACTTGTAAAAAAAAAAAACTACAAAAAAATCCTTAGAATCATTAAATTGTGTCCCTGTATTACCAAAATAAC
ACAGCACCGTGCATGTATAGTTTAATTGCAGTTTCATCTGTGAAAACGTGAAATTGTCTAGTCCTTCGTTATGTTCCCCAGATGTCTTCC
AGATTTGCTCTGCATGTGGTAACTTGTGTTAGGGCTGTGAGCTGTTCCTCGAGTTGAATGGGGATGTCAGTGCTCCTAGGGTTCTCCAGG
TGGTTCTTCAGACCTTCACCTGTGGGGGGGGGGGTAGGCGGTGCCCACGCCCATCTCCTCATCCTCCTGAACTTCTGCAACCCCACTGCT
GGGCAGACATCCTGGGCAACCCCTTTTTTCAGAGCAAGAAGTCATAAAGATAGGATTTCTTGGACATTTGGTTCTTATCAATATTGGGCA
TTATGTAATGACTTATTTACAAAACAAAGATACTGGAAAATGTTTTGGATGTGGTGTTATGGAAAGAGCACAGGCCTTGGACCCATCCAG
CTGGGTTCAGAACTACCCCCTGCTTATAACTGCGGCTGGCTGTGGGCCAGTCATTCTGCGTCTCTGCTTTCTTCCTCTGCTTCAGACTGT
CAGCTGTAAAGTGGAAGCAATATTACTTGCCTTGTATATGGTAAAGATTATAAAAATACATTTCAACTGTTCAGCATAGTACTTCAAAGC
AAGTACTCAGTAAATAGCAAGTCTTTTTAAATGCTGCTTTATTTCACTAAATTTTGTTGTGAGGTGTCACTAAAATGCCTGCAAACAAAC
GTAACTGCTAATCTGAGAGGAAACCCTCTTACTAATCAGAGAAGAAACCCTCCTGTCAGAAACCTTCAGGGAAGTGAGCTGATCACACCT
AAACTGGGAGTTTGCAATGGGGTATTTGAAGCACTGTGGGAGTATTCCACTGGCCCTCTCCCTGAGAGACTTAACAGTCTTCCCTGTTGT
CCAGATTCTGTATAAGGCAATCAGAATAATCATCTTCCTTGTTCAGCAGAGGAGCCTGGTCCCATTTTCCCCACTTTGTGATGGGCTTCT
CTCAGCGGTAGCTCAGCAGTTCCAGATGGCAGTTTGGACCAGCATCTAGGCTGGCCAGTTCGCTGTGTTTACTTAGAACCAACACGTTCA
GAGCTGGCCTGGACCATCTGAGGGGAACAGGAAACACCCCTAGGCTGTGGAAGCAAGTGCAGACCCCCACCCCCGGCCCTGAAGCCAAGG
GGGCAGGGTTTGGGAGTGGCCAAAGAGAAGCAGTGCAGGGATGGGTTTTCCTAGGGACAGGCTTAGCATTCCTGACTCTAGGAAGAAGGA
GCAGTGAGGCGGAGAAACAGTGGAGGGGATGGTGGCATTGGGCCCCATGGGGCCGAGATGGACACAGGGCTCGTTCTCTTGAGTCTGGTG
CCAAGGACAGCTGAAGACGACATCATTTTCAGGTGGAGAGGAGAGAGTGGAGGGAGATCATGCCCTGTGATGTGTCTTTTGCAGGTGAAG
GTGGGAGACAAGGTCTCTGCTGACGATGAGGCAGAGCCACCGTGAAAGTTGTAATAGGAGGACTGCCCGCCGCTGGAAGGGCCTGCAGTG
ACGCTAGGACACCCTCTGCCTGCATGTCACGTTAGCTGGGCTGGGCGAAGTAGAAGACCAAGGGGAAGAGGTGCAGTGGGGAGACCAGGT
GGGATGCAACCACAGGACCAGTGGAGGGGCTGTGGCACGTGGGCGGAGACTGAGTGGCTGGGCATGTGTTGTGGCTGAGCATGTGGTGTG
GGCAGTGGTCCTAGACCCCGCCATGTCCGGACAATGATATAGAGCGTCTCAGCATCGCCAGTCTAGACTGTCTATGGAGAGCAGAAAGTT
GTCTGGGGCTGCCTGGGGAACTGTGAGGCCAGCTATATCACCGTCGCTGATGGTGACATTACGGTGGTGGCAGGAGCAAGGAGAGAGGGA
AGAAGGACCCCGTCCAGCTTTAGTCACAAAATACCCAATGGAAGATGCCAGTGCCAATCCTGTGGGTTTCCTTGGGACTTCACACTGGCT
TTCTTATCTGCTCCAGATCCATTCAGTAGTCACTGAGTTCCTGCCAAATACTTTGTAGCGCCAGAAGCCAGGAGCGGGGTCTGCAGCAGG
GCAGTCCCCATTTTCAGGAAATGCCTGGAGCTGCTGGTCCCTGAGAGAAAGGAAAACATCTTTCAGCCGTACGCAGGCCAAGAAGGCCAA
TGTCCAGTAGCTTTGTGATTTTTTTTATATTTTTTTATTTATTTATTTTTGAGATAGAGTCTTGCTCTGTCGCTCAGGCTGGAGTGCAGT
GGCGTGATCTCCACTCACTGCAACTTCCGCCTCCTGGGGTCAAGCAGTTCTGCCTCAGCCTCCCGAGTAGCTGGGATTACAGGCACACGC
CACCACACCCAGCTAATTTTTGTGTTTTTAGTAGAGACGGTTTCACCATGTCGGCCAGGCTGGTCTCAAACTCCTGACCTCAGATGATTC
AGCCTCCCAAAGTGCTGGGATTACAGGTGTGAGCCACTGCACCCAGCCTGTGATGTTTCTGTGGGGTTCCACAAATGTGTGTGTGTGTAA
AAGCTGATGATTACAGCAAGAATGTGAACAGTAGCAGTTTTCCATTTGAAGGCAAGTTTTGTCTTTATCTGGGTATCAGAAGGACCCTCT
GGGCCATTGTCGCTTCCTGTACTCAGAGCCACCCTAGTACTACGGGCACACACAGAAAACAGCAGCCTGCGTACTTTCAAAGGAAAGGCA
TCTTTAATCACCAATGCCTGGAAAAATTATTTTGTTTCCCTCTTCCTTCCGTCTTGTTTCCTAACTTCTTACCAAAGTTTAGAGTCTGAG
TTTTTCGTATAATAATGTCCCACATCCACACATCGGGCCTACAGATGCTCTCCCTTGAATCGACTGGAAACATGACACCGGTTCCATGCT
CTGGAACTGTCACCTGTGATGTGCTGGGCTGTGTCCCAAGCACAGGAATCCCAGCAGTTTCAGCTCGATGCAGAACCACCATGCTCCAGA
CACAGGCTTGGGAAAGACACGTCAAAATTAAAATACTAGGTAAGAGAAGCACCTGATTGGGTAGAAGTTGGAGAGGAATCCTGGAATTTT
GTGGCCAGAAGGAGCCACTGCCCCTTTTGTTTAGTAAGACTAGACAGTAACAGAAGCCAGTTGTCAGCTATGAAAGTGGTGGGTGAAGCA
GGGGAGGCTCCTCTATGGTGGGACCCTGGACAAGGGAAGCCGAATGTGTGAAGAAGGGGTGCGGGGGTGTGCGGTGCCCTAGGACACTAG
GGCAAAGGTTTCAAACCTGGAACAAGGCACTGGAGGAAGATCTGCTGCCAGTCAGCAGTGCGGGCCCTCGAGTTAGCAGTCCGTGCGCAG
AGGGGCCAGTTCTGAGACCAGTTTGGAGAGTCAGGCAGTGACCCATTGGCCATGTCATAATTCCTTCAGCCTGCCTCCTCTTTAATCCCA
GAGAGTGCTCTTTCTTCATACTTCCTTTAAAATACTAAATTGTTCCCATTCCATGGGGAGCTGGCTAGGCTTTACAGGCTAGGAAATGTA
GGTTTTCTGAGATGGAACCATCTACACAAGGAGGAGGAAGGCACTAAGACTACAGATGAGACCCATGACAGGGCTGAGCATTTGGAAGCC
AACCCTGGTTGCTTTTCAAGAATTGCTTTGTGGCTGGGTGCAGTGGTTCACACCTGTAATTGCAGCACTTTGGGAGGCTGAGGCAGGTGG
ATTGCTTGAACCCAGGAGTTCGAGACCAGCCTGGGCAACATATGGGACACCCCACCGCCCCCGGCTCTGCAAAAAAATTAAAAATTAGCC
AGGCGTGGTGTCATGAGCCTGTGGTCTCAACTACTCAGGAGGCTGAGGCTGGAGGATCGCTTGAACCTGGGAGGTCAAGGCTCCAGTGAA
CCATAATTGTGCCACTGCACTGCAGCCTGGGCGACAATTTGTTTTCTAAATTGCTTTTGAAAGTCTACTGCATTACATATTCCAAAAAGC
AGTGGTTTTCAAATACTTTTATCACCGATATCCTTTTATGAAATGAAATCAGTAGAACTTTCTCTGCTCTGAATAAGCAAGGGTGGGAAC
CTGTCTACCTCCCACAGATAGCATAATGTGCCTGCCATAGAGGAGCCAAAAAATGGTGATGGGAACTGAGAGGAGAGCAAATGTCACAAA
AGACTGAGCAATTGAGAAAACAAAACAAGACCACAGATGACTGTTAACGCCTCCACAGTGGACCAAGAAAGGACAGAGAGCTGGCAGCAT
GGGCATCACTGTCTGGTCGGCAGCAGGAAGGCCTCGCTAGGGAATTGAGTACAGTCATCTAACTAGTTTAAAAGTACAGGAAGGATGATT
AAGGCTATTGGAGAGGTCATACAAATAGGGGAGGGGCAGGCAATGGCTGATAAGACATGAATTTGTAAGGCGATGAGTATTGCAGTCAGC
AAAACAAACGAGACTGCTCTCCCAACACATAACTCAGCAGGGAGGCCAGGCATTGGTTTAACCATTTAATATAAAGAAGTTAAAATTACA
AATGCGCTAAGTGCCTAAAGAAGAATAAGTGCAGGAATGAGAGCAGCATGGACTGCCACAGTTTTAGAATAAGCACTGTCACTGCTAGAT
TGGAAACAAAAATCCATAAATTTGGCCCGGTGTGGTGGCGGACGCCTGTAGTCCCAGCTACTTGGAGGCTGAGGCGTGAGAATCGCTTGA
ACCCGGGAGGCGGAGGTTGCAGTGAGCCGAGATGGCGCCACTGCACTCCAGCCTGGGCGTCAGAGTGAGAACTCTGTATCAAAAAATAAA
AATAAAAAAAAGTCCATAAATCTGCAATGTCTCAGTTAAGAAAGAAAGACTGGGCCAATGCAGATTTCAAACCGGAGAAAGTCATACTGT
CAGTGAAGGCCGCCTGTGGCCGGAAGGCGCCAGGGGATTAGCACCCTGGACTCAGTGTTGCTGGGAAACAGGGCCCCAAGGCTGGGAGCA
CAGTGTTTAAAGGGCATCTACCCAAGAAGGGAGCACAGGGCAAGGAGGAGCTGCAGGGGGGCTTGGCTGCCAAAGTGAATTCTGAGGAGA
GAGCTATTGCTGCCTACGATATGCAGGCTGCACAGAACACAAGTGGAATCAGCAGGCAGGAGAGGCAGCTAACGACGCAGCCCGTTTCTT
ATTTCTGTTTTCTCACAAGCGATGAAAGTGGAAAAGAGGGTGAGCAGGTGGCCCACACATGTGCCTCCAGTGCTGCGGCCCCTCCGGGGA
CCATCAGCCAGGCCCCGGGGAGGGAGCCAGCCACAGTGTGTCCGGCTCTTCTCTGAAGGGAAGAGAGCCTTGAATAGACTGAAGCGAAGA
CGGTTCTGCAAGGACAAGGCAGACCGAAGGCATTGGTTTTTTTTTTCAGATAAGGAGAATTAGACTCCCAAGTAGACACCAGAGTCACTG
TTTGGTTGGTGGGTGATAGTGGGGTCACAGTGGCTGCCTGTGCTCCCCCAGGGTGAGCGTGACTGTGCTAACCTGGGTGGGGCAGCATGC
ACACCCCTCTGGCAGCCCTTTGTTGCTCGCTGATGACAAGTTTGGATGATCCCGCCAAACAGCTTGCTAAGATGTAGTCCCCAGTGTTGG
AGGTGGGGCCTGATGGGAGGTGCTACCCTTGTGAGATAAGGTTGTGTAAAAGCCTGTGGCACCTCCCCACACTGACGCTCTCACCCCTGC
TCTGGCCATGTGCCGCGCCTGCTCCCACTTCCCCTTCTGCCAGGAGTAAAAGCCCCCGAGACCTCCCAGAAGCCAAGCAGATGCTAGTGC

>In-frame_ENST00000578105_ENST00000215832_TCGA-04-1365_SKA2_chr17_57232491_-_MAPK1_chr22_22162135_length(amino acids)=292AA_start in transcript=139_stop in transcript=1017
MREIKILLRFRHENIIGINDIIRAPTIEQMKDVYIVQDLMETDLYKLLKTQHLSNDHICYFLYQILRGLKYIHSANVLHRDLKPSNLLLN
TTCDLKICDFGLARVADPDHDHTGFLTEYVATRWYRAPEIMLNSKGYTKSIDIWSVGCILAEMLSNRPIFPGKHYLDQLNHILGILGSPS
QEDLNCIINLKARNYLLSLPHKNKVPWNRLFPNADSKALDLLDKMLTFNPHKRIEVEQALAHPYLEQYYDPSDEPIAEAPFKFDMELDDL

--------------------------------------------------------------
>In-frame_ENST00000581068_ENST00000215832_TCGA-04-1365_SKA2_chr17_57232491_-_MAPK1_chr22_22162135_length(transcript)=10832nt_BP=118nt
GACAGCTGTCCAATGGAGGCCCTCCGTGCTAGTACGGCTTGCGGCTTAAGCGCCGCCGCGGTCTGCGGAATGTCAACTATTCAACATGGA
GGCGGAGGTCGATAAGCTGGAACTGATGCTCTGCTTATGATAATGTCAACAAAGTTCGAGTAGCTATCAAGAAAATCAGCCCCTTTGAGC
ACCAGACCTACTGCCAGAGAACCCTGAGGGAGATAAAAATCTTACTGCGCTTCAGACATGAGAACATCATTGGAATCAATGACATTATTC
GAGCACCAACCATCGAGCAAATGAAAGATGTATATATAGTACAGGACCTCATGGAAACAGATCTTTACAAGCTCTTGAAGACACAACACC
TCAGCAATGACCATATCTGCTATTTTCTCTACCAGATCCTCAGAGGGTTAAAATATATCCATTCAGCTAACGTTCTGCACCGTGACCTCA
AGCCTTCCAACCTGCTGCTCAACACCACCTGTGATCTCAAGATCTGTGACTTTGGCCTGGCCCGTGTTGCAGATCCAGACCATGATCACA
CAGGGTTCCTGACAGAATATGTGGCCACACGTTGGTACAGGGCTCCAGAAATTATGTTGAATTCCAAGGGCTACACCAAGTCCATTGATA
TTTGGTCTGTAGGCTGCATTCTGGCAGAAATGCTTTCTAACAGGCCCATCTTTCCAGGGAAGCATTATCTTGACCAGCTGAACCACATTT
TGGGTATTCTTGGATCCCCATCACAAGAAGACCTGAATTGTATAATAAATTTAAAAGCTAGGAACTATTTGCTTTCTCTTCCACACAAAA
ATAAGGTGCCATGGAACAGGCTGTTCCCAAATGCTGACTCCAAAGCTCTGGACTTATTGGACAAAATGTTGACATTCAACCCACACAAGA
GGATTGAAGTAGAACAGGCTCTGGCCCACCCATATCTGGAGCAGTATTACGACCCGAGTGACGAGCCCATCGCCGAAGCACCATTCAAGT
TCGACATGGAATTGGATGACTTGCCTAAGGAAAAGCTCAAAGAACTAATTTTTGAAGAGACTGCTAGATTCCAGCCAGGATACAGATCTT
AAATTTGTCAGGACAAGGGCTCAGAGGACTGGACGTGCTCAGACATCGGTGTTCTTCTTCCCAGTTCTTGACCCCTGGTCCTGTCTCCAG
CCCGTCTTGGCTTATCCACTTTGACTCCTTTGAGCCGTTTGGAGGGGCGGTTTCTGGTAGTTGTGGCTTTTATGCTTTCAAAGAATTTCT
TCAGTCCAGAGAATTCCTCCTGGCAGCCCTGTGTGTGTCACCCATTGGTGACCTGCGGCAGTATGTACTTCAGTGCACCTACTGCTTACT
GTTGCTTTAGTCACTAATTGCTTTCTGGTTTGAAAGATGCAGTGGTTCCTCCCTCTCCTGAATCCTTTTCTACATGATGCCCTGCTGACC
ATGCAGCCGCACCAGAGAGAGATTCTTCCCCAATTGGCTCTAGTCACTGGCATCTCACTTTATGATAGGGAAGGCTACTACCTAGGGCAC
TTTAAGTCAGTGACAGCCCCTTATTTGCACTTCACCTTTTGACCATAACTGTTTCCCCAGAGCAGGAGCTTGTGGAAATACCTTGGCTGA
TGTTGCAGCCTGCAGCAAGTGCTTCCGTCTCCGGAATCCTTGGGGAGCACTTGTCCACGTCTTTTCTCATATCATGGTAGTCACTAACAT
ATATAAGGTATGTGCTATTGGCCCAGCTTTTAGAAAATGCAGTCATTTTTCTAAATAAAAAGGAAGTACTGCACCCAGCAGTGTCACTCT
GTAGTTACTGTGGTCACTTGTACCATATAGAGGTGTAACACTTGTCAAGAAGCGTTATGTGCAGTACTTAATGTTTGTAAGACTTACAAA
AAAAGATTTAAAGTGGCAGCTTCACTCGACATTTGGTGAGAGAAGTACAAAGGTTGCAGTGCTGAGCTGTGGGCGGTTTCTGGGGATGTC
CCAGGGTGGAACTCCACATGCTGGTGCATATACGCCCTTGAGCTACTTCAAATGTGGGTGTTTCAGTAACCACGTTCCATGCCTGAGGAT
TTAGCAGAGAGGAACACTGCGTCTTTAAATGAGAAAGTATACAATTCTTTTTCCTTCTACAGCATGTCAGCATCTCAAGTTCATTTTTCA
ACCTACAGTATAACAATTTGTAATAAAGCCTCCAGGAGCTCATGACGTGAAGCACTGTTCTGTCCTCAAGTACTCAAATATTTCTGATAC
TGCTGAGTCAGACTGTCAGAAAAAGCTAGCACTAACTCGTGTTTGGAGCTCTATCCATATTTTACTGATCTCTTTAAGTATTTGTTCCTG
CCACTGTGTACTGTGGAGTTGACTCGGTGTTCTGTCCCAGTGCGGTGCCTCCTCTTGACTTCCCCACTGCTCTCTGTGGTGAGAAATTTG
CCTTGTTCAATAATTACTGTACCCTCGCATGACTGTTACAGCTTTCTGTGCAGAGATGACTGTCCAAGTGCCACATGCCTACGATTGAAA
TGAAAACTCTATTGTTACCTCTGAGTTGTGTTCCACGGAAAATGCTATCCAGCAGATCATTTAGGAAAAATAATTCTATTTTTAGCTTTT
CATTTCTCAGCTGTCCTTTTTTCTTGTTTGATTTTTGACAGCAATGGAGAATGGGTTATATAAAGACTGCCTGCTAATATGAACAGAAAT
GCATTTGTAATTCATGAAAATAAATGTACATCTTCTATCTTCACATTCATGTTAAGATTCAGTGTTGCTTTCCTCTGGATCAGCGTGTCT
GAATGGACAGTCAGGTTCAGGTTGTGCTGAACACAGAAATGCTCACAGGCCTCACTTTGCCGCCCAGGCACTGGCCCAGCACTTGGATTT
ACATAAGATGAGTTAGAAAGGTACTTCTGTAGGGTCCTTTTTACCTCTGCTCGGCAGAGAATCGATGCTGTCATGTTCCTTTATTCACAA
TCTTAGGTCTCAAATATTCTGTCAAACCCTAACAAAGAAGCCCCGACATCTCAGGTTGGATTCCCTGGTTCTCTCTAAAGAGGGCCTGCC
CTTGTGCCCCAGAGGTGCTGCTGGGCACAGCCAAGAGTTGGGAAGGGCCGCCCCACAGTACGCAGTCCTCACCACCCAGCCCAGGGTGCT
CACGCTCACCACTCCTGTGGCTGAGGAAGGATAGCTGGCTCATCCTCGGAAAACAGACCCACATCTCTATTCTTGCCCTGAAATACGCGC
TTTTCACTTGCGTGCTCAGAGCTGCCGTCTGAAGGTCCACACAGCATTGACGGGACACAGAAATGTGACTGTTACCGGATAACACTGATT
AGTCAGTTTTCATTTATAAAAAAGCATTGACAGTTTTATTACTCTTGTTTCTTTTTAAATGGAAAGTTACTATTATAAGGTTAATTTGGA
GTCCTCTTCTAAATAGAAAACCATATCCTTGGCTACTAACATCTGGAGACTGTGAGCTCCTTCCCATTCCCCTTCCTGGTACTGTGGAGT
CAGATTGGCATGAAACCACTAACTTCATTCTAGAATCATTGTAGCCATAAGTTGTGTGCTTTTTATTAATCATGCCAAACATAATGTAAC
TGGGCAGAGAATGGTCCTAACCAAGGTACCTATGAAAAGCGCTAGCTATCATGTGTAGTAGATGCATCATTTTGGCTCTTCTTACATTTG
TAAAAATGTACAGATTAGGTCATCTTAATTCATATTAGTGACACGGAACAGCACCTCCACTATTTGTATGTTCAAATAAGCTTTCAGACT
AATAGCTTTTTTGGTGTCTAAAATGTAAGCAAAAAATTCCTGCTGAAACATTCCAGTCCTTTCATTTAGTATAAAAGAAATACTGAACAA
GCCAGTGGGATGGAATTGAAAGAACTAATCATGAGGACTCTGTCCTGACACAGGTCCTCAAAGCTAGCAGAGATACGCAGACATTGTGGC
ATCTGGGTAGAAGAATACTGTATTGTGTGTGCAGTGCACAGTGTGTGGTGTGTGCACACTCATTCCTTCTGCTCTTGGGCACAGGCAGTG
GGTGTAGAGGTAACCAGTAGCTTTGAGAAGCTACATGTAGCTCACCAGTGGTTTTCTCTAAGGAATCACAAAAGTAAACTACCCAACCAC
ATGCCACGTAATATTTCAGCCATTCAGAGGAAACTGTTTTCTCTTTATTTGCTTATATGTTAATATGGTTTTTAAATTGGTAACTTTTAT
ATAGTATGGTAACAGTATGTTAATACACACATACATACGCACACATGCTTTGGGTCCTTCCATAATACTTTTATATTTGTAAATCAATGT
TTTGGAGCAATCCCAAGTTTAAGGGAAATATTTTTGTAAATGTAATGGTTTTGAAAATCTGAGCAATCCTTTTGCTTATACATTTTTAAA
GCATTTGTGCTTTAAAATTGTTATGCTGGTGTTTGAAACATGATACTCCTGTGGTGCAGATGAGAAGCTATAACAGTGAATATGTGGTTT
CTCTTACGTCATCCACCTTGACATGATGGGTCAGAAACAAATGGAAATCCAGAGCAAGTCCTCCAGGGTTGCACCAGGTTTACCTAAAGC
TTGTTGCCTTTTCTTGTGCTGTTTATGCGTGTAGAGCACTCAAGAAAGTTCTGAAACTGCTTTGTATCTGCTTTGTACTGTTGGTGCCTT
CTTGGTATTGTACCCCAAAATTCTGCATAGATTATTTAGTATAATGGTAAGTTAAAAAATGTTAAAGGAAGATTTTATTAAGAATCTGAA
TGTTTATTCATTATATTGTTACAATTTAACATTAACATTTATTTGTGGTATTTGTGATTTGGTTAATCTGTATAAAAATTGTAAGTAGAA
AGGTTTATATTTCATCTTAATTCTTTTGATGTTGTAAACGTACTTTTTAAAAGATGGATTATTTGAATGTTTATGGCACCTGACTTGTAA
AAAAAAAAAACTACAAAAAAATCCTTAGAATCATTAAATTGTGTCCCTGTATTACCAAAATAACACAGCACCGTGCATGTATAGTTTAAT
TGCAGTTTCATCTGTGAAAACGTGAAATTGTCTAGTCCTTCGTTATGTTCCCCAGATGTCTTCCAGATTTGCTCTGCATGTGGTAACTTG
TGTTAGGGCTGTGAGCTGTTCCTCGAGTTGAATGGGGATGTCAGTGCTCCTAGGGTTCTCCAGGTGGTTCTTCAGACCTTCACCTGTGGG
GGGGGGGGTAGGCGGTGCCCACGCCCATCTCCTCATCCTCCTGAACTTCTGCAACCCCACTGCTGGGCAGACATCCTGGGCAACCCCTTT
TTTCAGAGCAAGAAGTCATAAAGATAGGATTTCTTGGACATTTGGTTCTTATCAATATTGGGCATTATGTAATGACTTATTTACAAAACA
AAGATACTGGAAAATGTTTTGGATGTGGTGTTATGGAAAGAGCACAGGCCTTGGACCCATCCAGCTGGGTTCAGAACTACCCCCTGCTTA
TAACTGCGGCTGGCTGTGGGCCAGTCATTCTGCGTCTCTGCTTTCTTCCTCTGCTTCAGACTGTCAGCTGTAAAGTGGAAGCAATATTAC
TTGCCTTGTATATGGTAAAGATTATAAAAATACATTTCAACTGTTCAGCATAGTACTTCAAAGCAAGTACTCAGTAAATAGCAAGTCTTT
TTAAATGCTGCTTTATTTCACTAAATTTTGTTGTGAGGTGTCACTAAAATGCCTGCAAACAAACGTAACTGCTAATCTGAGAGGAAACCC
TCTTACTAATCAGAGAAGAAACCCTCCTGTCAGAAACCTTCAGGGAAGTGAGCTGATCACACCTAAACTGGGAGTTTGCAATGGGGTATT
TGAAGCACTGTGGGAGTATTCCACTGGCCCTCTCCCTGAGAGACTTAACAGTCTTCCCTGTTGTCCAGATTCTGTATAAGGCAATCAGAA
TAATCATCTTCCTTGTTCAGCAGAGGAGCCTGGTCCCATTTTCCCCACTTTGTGATGGGCTTCTCTCAGCGGTAGCTCAGCAGTTCCAGA
TGGCAGTTTGGACCAGCATCTAGGCTGGCCAGTTCGCTGTGTTTACTTAGAACCAACACGTTCAGAGCTGGCCTGGACCATCTGAGGGGA
ACAGGAAACACCCCTAGGCTGTGGAAGCAAGTGCAGACCCCCACCCCCGGCCCTGAAGCCAAGGGGGCAGGGTTTGGGAGTGGCCAAAGA
GAAGCAGTGCAGGGATGGGTTTTCCTAGGGACAGGCTTAGCATTCCTGACTCTAGGAAGAAGGAGCAGTGAGGCGGAGAAACAGTGGAGG
GGATGGTGGCATTGGGCCCCATGGGGCCGAGATGGACACAGGGCTCGTTCTCTTGAGTCTGGTGCCAAGGACAGCTGAAGACGACATCAT
TTTCAGGTGGAGAGGAGAGAGTGGAGGGAGATCATGCCCTGTGATGTGTCTTTTGCAGGTGAAGGTGGGAGACAAGGTCTCTGCTGACGA
TGAGGCAGAGCCACCGTGAAAGTTGTAATAGGAGGACTGCCCGCCGCTGGAAGGGCCTGCAGTGACGCTAGGACACCCTCTGCCTGCATG
TCACGTTAGCTGGGCTGGGCGAAGTAGAAGACCAAGGGGAAGAGGTGCAGTGGGGAGACCAGGTGGGATGCAACCACAGGACCAGTGGAG
GGGCTGTGGCACGTGGGCGGAGACTGAGTGGCTGGGCATGTGTTGTGGCTGAGCATGTGGTGTGGGCAGTGGTCCTAGACCCCGCCATGT
CCGGACAATGATATAGAGCGTCTCAGCATCGCCAGTCTAGACTGTCTATGGAGAGCAGAAAGTTGTCTGGGGCTGCCTGGGGAACTGTGA
GGCCAGCTATATCACCGTCGCTGATGGTGACATTACGGTGGTGGCAGGAGCAAGGAGAGAGGGAAGAAGGACCCCGTCCAGCTTTAGTCA
CAAAATACCCAATGGAAGATGCCAGTGCCAATCCTGTGGGTTTCCTTGGGACTTCACACTGGCTTTCTTATCTGCTCCAGATCCATTCAG
TAGTCACTGAGTTCCTGCCAAATACTTTGTAGCGCCAGAAGCCAGGAGCGGGGTCTGCAGCAGGGCAGTCCCCATTTTCAGGAAATGCCT
GGAGCTGCTGGTCCCTGAGAGAAAGGAAAACATCTTTCAGCCGTACGCAGGCCAAGAAGGCCAATGTCCAGTAGCTTTGTGATTTTTTTT
ATATTTTTTTATTTATTTATTTTTGAGATAGAGTCTTGCTCTGTCGCTCAGGCTGGAGTGCAGTGGCGTGATCTCCACTCACTGCAACTT
CCGCCTCCTGGGGTCAAGCAGTTCTGCCTCAGCCTCCCGAGTAGCTGGGATTACAGGCACACGCCACCACACCCAGCTAATTTTTGTGTT
TTTAGTAGAGACGGTTTCACCATGTCGGCCAGGCTGGTCTCAAACTCCTGACCTCAGATGATTCAGCCTCCCAAAGTGCTGGGATTACAG
GTGTGAGCCACTGCACCCAGCCTGTGATGTTTCTGTGGGGTTCCACAAATGTGTGTGTGTGTAAAAGCTGATGATTACAGCAAGAATGTG
AACAGTAGCAGTTTTCCATTTGAAGGCAAGTTTTGTCTTTATCTGGGTATCAGAAGGACCCTCTGGGCCATTGTCGCTTCCTGTACTCAG
AGCCACCCTAGTACTACGGGCACACACAGAAAACAGCAGCCTGCGTACTTTCAAAGGAAAGGCATCTTTAATCACCAATGCCTGGAAAAA
TTATTTTGTTTCCCTCTTCCTTCCGTCTTGTTTCCTAACTTCTTACCAAAGTTTAGAGTCTGAGTTTTTCGTATAATAATGTCCCACATC
CACACATCGGGCCTACAGATGCTCTCCCTTGAATCGACTGGAAACATGACACCGGTTCCATGCTCTGGAACTGTCACCTGTGATGTGCTG
GGCTGTGTCCCAAGCACAGGAATCCCAGCAGTTTCAGCTCGATGCAGAACCACCATGCTCCAGACACAGGCTTGGGAAAGACACGTCAAA
ATTAAAATACTAGGTAAGAGAAGCACCTGATTGGGTAGAAGTTGGAGAGGAATCCTGGAATTTTGTGGCCAGAAGGAGCCACTGCCCCTT
TTGTTTAGTAAGACTAGACAGTAACAGAAGCCAGTTGTCAGCTATGAAAGTGGTGGGTGAAGCAGGGGAGGCTCCTCTATGGTGGGACCC
TGGACAAGGGAAGCCGAATGTGTGAAGAAGGGGTGCGGGGGTGTGCGGTGCCCTAGGACACTAGGGCAAAGGTTTCAAACCTGGAACAAG
GCACTGGAGGAAGATCTGCTGCCAGTCAGCAGTGCGGGCCCTCGAGTTAGCAGTCCGTGCGCAGAGGGGCCAGTTCTGAGACCAGTTTGG
AGAGTCAGGCAGTGACCCATTGGCCATGTCATAATTCCTTCAGCCTGCCTCCTCTTTAATCCCAGAGAGTGCTCTTTCTTCATACTTCCT
TTAAAATACTAAATTGTTCCCATTCCATGGGGAGCTGGCTAGGCTTTACAGGCTAGGAAATGTAGGTTTTCTGAGATGGAACCATCTACA
CAAGGAGGAGGAAGGCACTAAGACTACAGATGAGACCCATGACAGGGCTGAGCATTTGGAAGCCAACCCTGGTTGCTTTTCAAGAATTGC
TTTGTGGCTGGGTGCAGTGGTTCACACCTGTAATTGCAGCACTTTGGGAGGCTGAGGCAGGTGGATTGCTTGAACCCAGGAGTTCGAGAC
CAGCCTGGGCAACATATGGGACACCCCACCGCCCCCGGCTCTGCAAAAAAATTAAAAATTAGCCAGGCGTGGTGTCATGAGCCTGTGGTC
TCAACTACTCAGGAGGCTGAGGCTGGAGGATCGCTTGAACCTGGGAGGTCAAGGCTCCAGTGAACCATAATTGTGCCACTGCACTGCAGC
CTGGGCGACAATTTGTTTTCTAAATTGCTTTTGAAAGTCTACTGCATTACATATTCCAAAAAGCAGTGGTTTTCAAATACTTTTATCACC
GATATCCTTTTATGAAATGAAATCAGTAGAACTTTCTCTGCTCTGAATAAGCAAGGGTGGGAACCTGTCTACCTCCCACAGATAGCATAA
TGTGCCTGCCATAGAGGAGCCAAAAAATGGTGATGGGAACTGAGAGGAGAGCAAATGTCACAAAAGACTGAGCAATTGAGAAAACAAAAC
AAGACCACAGATGACTGTTAACGCCTCCACAGTGGACCAAGAAAGGACAGAGAGCTGGCAGCATGGGCATCACTGTCTGGTCGGCAGCAG
GAAGGCCTCGCTAGGGAATTGAGTACAGTCATCTAACTAGTTTAAAAGTACAGGAAGGATGATTAAGGCTATTGGAGAGGTCATACAAAT
AGGGGAGGGGCAGGCAATGGCTGATAAGACATGAATTTGTAAGGCGATGAGTATTGCAGTCAGCAAAACAAACGAGACTGCTCTCCCAAC
ACATAACTCAGCAGGGAGGCCAGGCATTGGTTTAACCATTTAATATAAAGAAGTTAAAATTACAAATGCGCTAAGTGCCTAAAGAAGAAT
AAGTGCAGGAATGAGAGCAGCATGGACTGCCACAGTTTTAGAATAAGCACTGTCACTGCTAGATTGGAAACAAAAATCCATAAATTTGGC
CCGGTGTGGTGGCGGACGCCTGTAGTCCCAGCTACTTGGAGGCTGAGGCGTGAGAATCGCTTGAACCCGGGAGGCGGAGGTTGCAGTGAG
CCGAGATGGCGCCACTGCACTCCAGCCTGGGCGTCAGAGTGAGAACTCTGTATCAAAAAATAAAAATAAAAAAAAGTCCATAAATCTGCA
ATGTCTCAGTTAAGAAAGAAAGACTGGGCCAATGCAGATTTCAAACCGGAGAAAGTCATACTGTCAGTGAAGGCCGCCTGTGGCCGGAAG
GCGCCAGGGGATTAGCACCCTGGACTCAGTGTTGCTGGGAAACAGGGCCCCAAGGCTGGGAGCACAGTGTTTAAAGGGCATCTACCCAAG
AAGGGAGCACAGGGCAAGGAGGAGCTGCAGGGGGGCTTGGCTGCCAAAGTGAATTCTGAGGAGAGAGCTATTGCTGCCTACGATATGCAG
GCTGCACAGAACACAAGTGGAATCAGCAGGCAGGAGAGGCAGCTAACGACGCAGCCCGTTTCTTATTTCTGTTTTCTCACAAGCGATGAA
AGTGGAAAAGAGGGTGAGCAGGTGGCCCACACATGTGCCTCCAGTGCTGCGGCCCCTCCGGGGACCATCAGCCAGGCCCCGGGGAGGGAG
CCAGCCACAGTGTGTCCGGCTCTTCTCTGAAGGGAAGAGAGCCTTGAATAGACTGAAGCGAAGACGGTTCTGCAAGGACAAGGCAGACCG
AAGGCATTGGTTTTTTTTTTCAGATAAGGAGAATTAGACTCCCAAGTAGACACCAGAGTCACTGTTTGGTTGGTGGGTGATAGTGGGGTC
ACAGTGGCTGCCTGTGCTCCCCCAGGGTGAGCGTGACTGTGCTAACCTGGGTGGGGCAGCATGCACACCCCTCTGGCAGCCCTTTGTTGC
TCGCTGATGACAAGTTTGGATGATCCCGCCAAACAGCTTGCTAAGATGTAGTCCCCAGTGTTGGAGGTGGGGCCTGATGGGAGGTGCTAC
CCTTGTGAGATAAGGTTGTGTAAAAGCCTGTGGCACCTCCCCACACTGACGCTCTCACCCCTGCTCTGGCCATGTGCCGCGCCTGCTCCC
ACTTCCCCTTCTGCCAGGAGTAAAAGCCCCCGAGACCTCCCAGAAGCCAAGCAGATGCTAGTGCCATGCTTCCTCTGCAGCCTGCAGAAC

>In-frame_ENST00000581068_ENST00000215832_TCGA-04-1365_SKA2_chr17_57232491_-_MAPK1_chr22_22162135_length(amino acids)=292AA_start in transcript=203_stop in transcript=1081
MREIKILLRFRHENIIGINDIIRAPTIEQMKDVYIVQDLMETDLYKLLKTQHLSNDHICYFLYQILRGLKYIHSANVLHRDLKPSNLLLN
TTCDLKICDFGLARVADPDHDHTGFLTEYVATRWYRAPEIMLNSKGYTKSIDIWSVGCILAEMLSNRPIFPGKHYLDQLNHILGILGSPS
QEDLNCIINLKARNYLLSLPHKNKVPWNRLFPNADSKALDLLDKMLTFNPHKRIEVEQALAHPYLEQYYDPSDEPIAEAPFKFDMELDDL

--------------------------------------------------------------
>In-frame_ENST00000583380_ENST00000215832_TCGA-04-1365_SKA2_chr17_57232491_-_MAPK1_chr22_22162135_length(transcript)=10758nt_BP=44nt
AACTATTCAACATGGAGGCGGAGGTCGATAAGCTGGAACTGATGCTCTGCTTATGATAATGTCAACAAAGTTCGAGTAGCTATCAAGAAA
ATCAGCCCCTTTGAGCACCAGACCTACTGCCAGAGAACCCTGAGGGAGATAAAAATCTTACTGCGCTTCAGACATGAGAACATCATTGGA
ATCAATGACATTATTCGAGCACCAACCATCGAGCAAATGAAAGATGTATATATAGTACAGGACCTCATGGAAACAGATCTTTACAAGCTC
TTGAAGACACAACACCTCAGCAATGACCATATCTGCTATTTTCTCTACCAGATCCTCAGAGGGTTAAAATATATCCATTCAGCTAACGTT
CTGCACCGTGACCTCAAGCCTTCCAACCTGCTGCTCAACACCACCTGTGATCTCAAGATCTGTGACTTTGGCCTGGCCCGTGTTGCAGAT
CCAGACCATGATCACACAGGGTTCCTGACAGAATATGTGGCCACACGTTGGTACAGGGCTCCAGAAATTATGTTGAATTCCAAGGGCTAC
ACCAAGTCCATTGATATTTGGTCTGTAGGCTGCATTCTGGCAGAAATGCTTTCTAACAGGCCCATCTTTCCAGGGAAGCATTATCTTGAC
CAGCTGAACCACATTTTGGGTATTCTTGGATCCCCATCACAAGAAGACCTGAATTGTATAATAAATTTAAAAGCTAGGAACTATTTGCTT
TCTCTTCCACACAAAAATAAGGTGCCATGGAACAGGCTGTTCCCAAATGCTGACTCCAAAGCTCTGGACTTATTGGACAAAATGTTGACA
TTCAACCCACACAAGAGGATTGAAGTAGAACAGGCTCTGGCCCACCCATATCTGGAGCAGTATTACGACCCGAGTGACGAGCCCATCGCC
GAAGCACCATTCAAGTTCGACATGGAATTGGATGACTTGCCTAAGGAAAAGCTCAAAGAACTAATTTTTGAAGAGACTGCTAGATTCCAG
CCAGGATACAGATCTTAAATTTGTCAGGACAAGGGCTCAGAGGACTGGACGTGCTCAGACATCGGTGTTCTTCTTCCCAGTTCTTGACCC
CTGGTCCTGTCTCCAGCCCGTCTTGGCTTATCCACTTTGACTCCTTTGAGCCGTTTGGAGGGGCGGTTTCTGGTAGTTGTGGCTTTTATG
CTTTCAAAGAATTTCTTCAGTCCAGAGAATTCCTCCTGGCAGCCCTGTGTGTGTCACCCATTGGTGACCTGCGGCAGTATGTACTTCAGT
GCACCTACTGCTTACTGTTGCTTTAGTCACTAATTGCTTTCTGGTTTGAAAGATGCAGTGGTTCCTCCCTCTCCTGAATCCTTTTCTACA
TGATGCCCTGCTGACCATGCAGCCGCACCAGAGAGAGATTCTTCCCCAATTGGCTCTAGTCACTGGCATCTCACTTTATGATAGGGAAGG
CTACTACCTAGGGCACTTTAAGTCAGTGACAGCCCCTTATTTGCACTTCACCTTTTGACCATAACTGTTTCCCCAGAGCAGGAGCTTGTG
GAAATACCTTGGCTGATGTTGCAGCCTGCAGCAAGTGCTTCCGTCTCCGGAATCCTTGGGGAGCACTTGTCCACGTCTTTTCTCATATCA
TGGTAGTCACTAACATATATAAGGTATGTGCTATTGGCCCAGCTTTTAGAAAATGCAGTCATTTTTCTAAATAAAAAGGAAGTACTGCAC
CCAGCAGTGTCACTCTGTAGTTACTGTGGTCACTTGTACCATATAGAGGTGTAACACTTGTCAAGAAGCGTTATGTGCAGTACTTAATGT
TTGTAAGACTTACAAAAAAAGATTTAAAGTGGCAGCTTCACTCGACATTTGGTGAGAGAAGTACAAAGGTTGCAGTGCTGAGCTGTGGGC
GGTTTCTGGGGATGTCCCAGGGTGGAACTCCACATGCTGGTGCATATACGCCCTTGAGCTACTTCAAATGTGGGTGTTTCAGTAACCACG
TTCCATGCCTGAGGATTTAGCAGAGAGGAACACTGCGTCTTTAAATGAGAAAGTATACAATTCTTTTTCCTTCTACAGCATGTCAGCATC
TCAAGTTCATTTTTCAACCTACAGTATAACAATTTGTAATAAAGCCTCCAGGAGCTCATGACGTGAAGCACTGTTCTGTCCTCAAGTACT
CAAATATTTCTGATACTGCTGAGTCAGACTGTCAGAAAAAGCTAGCACTAACTCGTGTTTGGAGCTCTATCCATATTTTACTGATCTCTT
TAAGTATTTGTTCCTGCCACTGTGTACTGTGGAGTTGACTCGGTGTTCTGTCCCAGTGCGGTGCCTCCTCTTGACTTCCCCACTGCTCTC
TGTGGTGAGAAATTTGCCTTGTTCAATAATTACTGTACCCTCGCATGACTGTTACAGCTTTCTGTGCAGAGATGACTGTCCAAGTGCCAC
ATGCCTACGATTGAAATGAAAACTCTATTGTTACCTCTGAGTTGTGTTCCACGGAAAATGCTATCCAGCAGATCATTTAGGAAAAATAAT
TCTATTTTTAGCTTTTCATTTCTCAGCTGTCCTTTTTTCTTGTTTGATTTTTGACAGCAATGGAGAATGGGTTATATAAAGACTGCCTGC
TAATATGAACAGAAATGCATTTGTAATTCATGAAAATAAATGTACATCTTCTATCTTCACATTCATGTTAAGATTCAGTGTTGCTTTCCT
CTGGATCAGCGTGTCTGAATGGACAGTCAGGTTCAGGTTGTGCTGAACACAGAAATGCTCACAGGCCTCACTTTGCCGCCCAGGCACTGG
CCCAGCACTTGGATTTACATAAGATGAGTTAGAAAGGTACTTCTGTAGGGTCCTTTTTACCTCTGCTCGGCAGAGAATCGATGCTGTCAT
GTTCCTTTATTCACAATCTTAGGTCTCAAATATTCTGTCAAACCCTAACAAAGAAGCCCCGACATCTCAGGTTGGATTCCCTGGTTCTCT
CTAAAGAGGGCCTGCCCTTGTGCCCCAGAGGTGCTGCTGGGCACAGCCAAGAGTTGGGAAGGGCCGCCCCACAGTACGCAGTCCTCACCA
CCCAGCCCAGGGTGCTCACGCTCACCACTCCTGTGGCTGAGGAAGGATAGCTGGCTCATCCTCGGAAAACAGACCCACATCTCTATTCTT
GCCCTGAAATACGCGCTTTTCACTTGCGTGCTCAGAGCTGCCGTCTGAAGGTCCACACAGCATTGACGGGACACAGAAATGTGACTGTTA
CCGGATAACACTGATTAGTCAGTTTTCATTTATAAAAAAGCATTGACAGTTTTATTACTCTTGTTTCTTTTTAAATGGAAAGTTACTATT
ATAAGGTTAATTTGGAGTCCTCTTCTAAATAGAAAACCATATCCTTGGCTACTAACATCTGGAGACTGTGAGCTCCTTCCCATTCCCCTT
CCTGGTACTGTGGAGTCAGATTGGCATGAAACCACTAACTTCATTCTAGAATCATTGTAGCCATAAGTTGTGTGCTTTTTATTAATCATG
CCAAACATAATGTAACTGGGCAGAGAATGGTCCTAACCAAGGTACCTATGAAAAGCGCTAGCTATCATGTGTAGTAGATGCATCATTTTG
GCTCTTCTTACATTTGTAAAAATGTACAGATTAGGTCATCTTAATTCATATTAGTGACACGGAACAGCACCTCCACTATTTGTATGTTCA
AATAAGCTTTCAGACTAATAGCTTTTTTGGTGTCTAAAATGTAAGCAAAAAATTCCTGCTGAAACATTCCAGTCCTTTCATTTAGTATAA
AAGAAATACTGAACAAGCCAGTGGGATGGAATTGAAAGAACTAATCATGAGGACTCTGTCCTGACACAGGTCCTCAAAGCTAGCAGAGAT
ACGCAGACATTGTGGCATCTGGGTAGAAGAATACTGTATTGTGTGTGCAGTGCACAGTGTGTGGTGTGTGCACACTCATTCCTTCTGCTC
TTGGGCACAGGCAGTGGGTGTAGAGGTAACCAGTAGCTTTGAGAAGCTACATGTAGCTCACCAGTGGTTTTCTCTAAGGAATCACAAAAG
TAAACTACCCAACCACATGCCACGTAATATTTCAGCCATTCAGAGGAAACTGTTTTCTCTTTATTTGCTTATATGTTAATATGGTTTTTA
AATTGGTAACTTTTATATAGTATGGTAACAGTATGTTAATACACACATACATACGCACACATGCTTTGGGTCCTTCCATAATACTTTTAT
ATTTGTAAATCAATGTTTTGGAGCAATCCCAAGTTTAAGGGAAATATTTTTGTAAATGTAATGGTTTTGAAAATCTGAGCAATCCTTTTG
CTTATACATTTTTAAAGCATTTGTGCTTTAAAATTGTTATGCTGGTGTTTGAAACATGATACTCCTGTGGTGCAGATGAGAAGCTATAAC
AGTGAATATGTGGTTTCTCTTACGTCATCCACCTTGACATGATGGGTCAGAAACAAATGGAAATCCAGAGCAAGTCCTCCAGGGTTGCAC
CAGGTTTACCTAAAGCTTGTTGCCTTTTCTTGTGCTGTTTATGCGTGTAGAGCACTCAAGAAAGTTCTGAAACTGCTTTGTATCTGCTTT
GTACTGTTGGTGCCTTCTTGGTATTGTACCCCAAAATTCTGCATAGATTATTTAGTATAATGGTAAGTTAAAAAATGTTAAAGGAAGATT
TTATTAAGAATCTGAATGTTTATTCATTATATTGTTACAATTTAACATTAACATTTATTTGTGGTATTTGTGATTTGGTTAATCTGTATA
AAAATTGTAAGTAGAAAGGTTTATATTTCATCTTAATTCTTTTGATGTTGTAAACGTACTTTTTAAAAGATGGATTATTTGAATGTTTAT
GGCACCTGACTTGTAAAAAAAAAAAACTACAAAAAAATCCTTAGAATCATTAAATTGTGTCCCTGTATTACCAAAATAACACAGCACCGT
GCATGTATAGTTTAATTGCAGTTTCATCTGTGAAAACGTGAAATTGTCTAGTCCTTCGTTATGTTCCCCAGATGTCTTCCAGATTTGCTC
TGCATGTGGTAACTTGTGTTAGGGCTGTGAGCTGTTCCTCGAGTTGAATGGGGATGTCAGTGCTCCTAGGGTTCTCCAGGTGGTTCTTCA
GACCTTCACCTGTGGGGGGGGGGGTAGGCGGTGCCCACGCCCATCTCCTCATCCTCCTGAACTTCTGCAACCCCACTGCTGGGCAGACAT
CCTGGGCAACCCCTTTTTTCAGAGCAAGAAGTCATAAAGATAGGATTTCTTGGACATTTGGTTCTTATCAATATTGGGCATTATGTAATG
ACTTATTTACAAAACAAAGATACTGGAAAATGTTTTGGATGTGGTGTTATGGAAAGAGCACAGGCCTTGGACCCATCCAGCTGGGTTCAG
AACTACCCCCTGCTTATAACTGCGGCTGGCTGTGGGCCAGTCATTCTGCGTCTCTGCTTTCTTCCTCTGCTTCAGACTGTCAGCTGTAAA
GTGGAAGCAATATTACTTGCCTTGTATATGGTAAAGATTATAAAAATACATTTCAACTGTTCAGCATAGTACTTCAAAGCAAGTACTCAG
TAAATAGCAAGTCTTTTTAAATGCTGCTTTATTTCACTAAATTTTGTTGTGAGGTGTCACTAAAATGCCTGCAAACAAACGTAACTGCTA
ATCTGAGAGGAAACCCTCTTACTAATCAGAGAAGAAACCCTCCTGTCAGAAACCTTCAGGGAAGTGAGCTGATCACACCTAAACTGGGAG
TTTGCAATGGGGTATTTGAAGCACTGTGGGAGTATTCCACTGGCCCTCTCCCTGAGAGACTTAACAGTCTTCCCTGTTGTCCAGATTCTG
TATAAGGCAATCAGAATAATCATCTTCCTTGTTCAGCAGAGGAGCCTGGTCCCATTTTCCCCACTTTGTGATGGGCTTCTCTCAGCGGTA
GCTCAGCAGTTCCAGATGGCAGTTTGGACCAGCATCTAGGCTGGCCAGTTCGCTGTGTTTACTTAGAACCAACACGTTCAGAGCTGGCCT
GGACCATCTGAGGGGAACAGGAAACACCCCTAGGCTGTGGAAGCAAGTGCAGACCCCCACCCCCGGCCCTGAAGCCAAGGGGGCAGGGTT
TGGGAGTGGCCAAAGAGAAGCAGTGCAGGGATGGGTTTTCCTAGGGACAGGCTTAGCATTCCTGACTCTAGGAAGAAGGAGCAGTGAGGC
GGAGAAACAGTGGAGGGGATGGTGGCATTGGGCCCCATGGGGCCGAGATGGACACAGGGCTCGTTCTCTTGAGTCTGGTGCCAAGGACAG
CTGAAGACGACATCATTTTCAGGTGGAGAGGAGAGAGTGGAGGGAGATCATGCCCTGTGATGTGTCTTTTGCAGGTGAAGGTGGGAGACA
AGGTCTCTGCTGACGATGAGGCAGAGCCACCGTGAAAGTTGTAATAGGAGGACTGCCCGCCGCTGGAAGGGCCTGCAGTGACGCTAGGAC
ACCCTCTGCCTGCATGTCACGTTAGCTGGGCTGGGCGAAGTAGAAGACCAAGGGGAAGAGGTGCAGTGGGGAGACCAGGTGGGATGCAAC
CACAGGACCAGTGGAGGGGCTGTGGCACGTGGGCGGAGACTGAGTGGCTGGGCATGTGTTGTGGCTGAGCATGTGGTGTGGGCAGTGGTC
CTAGACCCCGCCATGTCCGGACAATGATATAGAGCGTCTCAGCATCGCCAGTCTAGACTGTCTATGGAGAGCAGAAAGTTGTCTGGGGCT
GCCTGGGGAACTGTGAGGCCAGCTATATCACCGTCGCTGATGGTGACATTACGGTGGTGGCAGGAGCAAGGAGAGAGGGAAGAAGGACCC
CGTCCAGCTTTAGTCACAAAATACCCAATGGAAGATGCCAGTGCCAATCCTGTGGGTTTCCTTGGGACTTCACACTGGCTTTCTTATCTG
CTCCAGATCCATTCAGTAGTCACTGAGTTCCTGCCAAATACTTTGTAGCGCCAGAAGCCAGGAGCGGGGTCTGCAGCAGGGCAGTCCCCA
TTTTCAGGAAATGCCTGGAGCTGCTGGTCCCTGAGAGAAAGGAAAACATCTTTCAGCCGTACGCAGGCCAAGAAGGCCAATGTCCAGTAG
CTTTGTGATTTTTTTTATATTTTTTTATTTATTTATTTTTGAGATAGAGTCTTGCTCTGTCGCTCAGGCTGGAGTGCAGTGGCGTGATCT
CCACTCACTGCAACTTCCGCCTCCTGGGGTCAAGCAGTTCTGCCTCAGCCTCCCGAGTAGCTGGGATTACAGGCACACGCCACCACACCC
AGCTAATTTTTGTGTTTTTAGTAGAGACGGTTTCACCATGTCGGCCAGGCTGGTCTCAAACTCCTGACCTCAGATGATTCAGCCTCCCAA
AGTGCTGGGATTACAGGTGTGAGCCACTGCACCCAGCCTGTGATGTTTCTGTGGGGTTCCACAAATGTGTGTGTGTGTAAAAGCTGATGA
TTACAGCAAGAATGTGAACAGTAGCAGTTTTCCATTTGAAGGCAAGTTTTGTCTTTATCTGGGTATCAGAAGGACCCTCTGGGCCATTGT
CGCTTCCTGTACTCAGAGCCACCCTAGTACTACGGGCACACACAGAAAACAGCAGCCTGCGTACTTTCAAAGGAAAGGCATCTTTAATCA
CCAATGCCTGGAAAAATTATTTTGTTTCCCTCTTCCTTCCGTCTTGTTTCCTAACTTCTTACCAAAGTTTAGAGTCTGAGTTTTTCGTAT
AATAATGTCCCACATCCACACATCGGGCCTACAGATGCTCTCCCTTGAATCGACTGGAAACATGACACCGGTTCCATGCTCTGGAACTGT
CACCTGTGATGTGCTGGGCTGTGTCCCAAGCACAGGAATCCCAGCAGTTTCAGCTCGATGCAGAACCACCATGCTCCAGACACAGGCTTG
GGAAAGACACGTCAAAATTAAAATACTAGGTAAGAGAAGCACCTGATTGGGTAGAAGTTGGAGAGGAATCCTGGAATTTTGTGGCCAGAA
GGAGCCACTGCCCCTTTTGTTTAGTAAGACTAGACAGTAACAGAAGCCAGTTGTCAGCTATGAAAGTGGTGGGTGAAGCAGGGGAGGCTC
CTCTATGGTGGGACCCTGGACAAGGGAAGCCGAATGTGTGAAGAAGGGGTGCGGGGGTGTGCGGTGCCCTAGGACACTAGGGCAAAGGTT
TCAAACCTGGAACAAGGCACTGGAGGAAGATCTGCTGCCAGTCAGCAGTGCGGGCCCTCGAGTTAGCAGTCCGTGCGCAGAGGGGCCAGT
TCTGAGACCAGTTTGGAGAGTCAGGCAGTGACCCATTGGCCATGTCATAATTCCTTCAGCCTGCCTCCTCTTTAATCCCAGAGAGTGCTC
TTTCTTCATACTTCCTTTAAAATACTAAATTGTTCCCATTCCATGGGGAGCTGGCTAGGCTTTACAGGCTAGGAAATGTAGGTTTTCTGA
GATGGAACCATCTACACAAGGAGGAGGAAGGCACTAAGACTACAGATGAGACCCATGACAGGGCTGAGCATTTGGAAGCCAACCCTGGTT
GCTTTTCAAGAATTGCTTTGTGGCTGGGTGCAGTGGTTCACACCTGTAATTGCAGCACTTTGGGAGGCTGAGGCAGGTGGATTGCTTGAA
CCCAGGAGTTCGAGACCAGCCTGGGCAACATATGGGACACCCCACCGCCCCCGGCTCTGCAAAAAAATTAAAAATTAGCCAGGCGTGGTG
TCATGAGCCTGTGGTCTCAACTACTCAGGAGGCTGAGGCTGGAGGATCGCTTGAACCTGGGAGGTCAAGGCTCCAGTGAACCATAATTGT
GCCACTGCACTGCAGCCTGGGCGACAATTTGTTTTCTAAATTGCTTTTGAAAGTCTACTGCATTACATATTCCAAAAAGCAGTGGTTTTC
AAATACTTTTATCACCGATATCCTTTTATGAAATGAAATCAGTAGAACTTTCTCTGCTCTGAATAAGCAAGGGTGGGAACCTGTCTACCT
CCCACAGATAGCATAATGTGCCTGCCATAGAGGAGCCAAAAAATGGTGATGGGAACTGAGAGGAGAGCAAATGTCACAAAAGACTGAGCA
ATTGAGAAAACAAAACAAGACCACAGATGACTGTTAACGCCTCCACAGTGGACCAAGAAAGGACAGAGAGCTGGCAGCATGGGCATCACT
GTCTGGTCGGCAGCAGGAAGGCCTCGCTAGGGAATTGAGTACAGTCATCTAACTAGTTTAAAAGTACAGGAAGGATGATTAAGGCTATTG
GAGAGGTCATACAAATAGGGGAGGGGCAGGCAATGGCTGATAAGACATGAATTTGTAAGGCGATGAGTATTGCAGTCAGCAAAACAAACG
AGACTGCTCTCCCAACACATAACTCAGCAGGGAGGCCAGGCATTGGTTTAACCATTTAATATAAAGAAGTTAAAATTACAAATGCGCTAA
GTGCCTAAAGAAGAATAAGTGCAGGAATGAGAGCAGCATGGACTGCCACAGTTTTAGAATAAGCACTGTCACTGCTAGATTGGAAACAAA
AATCCATAAATTTGGCCCGGTGTGGTGGCGGACGCCTGTAGTCCCAGCTACTTGGAGGCTGAGGCGTGAGAATCGCTTGAACCCGGGAGG
CGGAGGTTGCAGTGAGCCGAGATGGCGCCACTGCACTCCAGCCTGGGCGTCAGAGTGAGAACTCTGTATCAAAAAATAAAAATAAAAAAA
AGTCCATAAATCTGCAATGTCTCAGTTAAGAAAGAAAGACTGGGCCAATGCAGATTTCAAACCGGAGAAAGTCATACTGTCAGTGAAGGC
CGCCTGTGGCCGGAAGGCGCCAGGGGATTAGCACCCTGGACTCAGTGTTGCTGGGAAACAGGGCCCCAAGGCTGGGAGCACAGTGTTTAA
AGGGCATCTACCCAAGAAGGGAGCACAGGGCAAGGAGGAGCTGCAGGGGGGCTTGGCTGCCAAAGTGAATTCTGAGGAGAGAGCTATTGC
TGCCTACGATATGCAGGCTGCACAGAACACAAGTGGAATCAGCAGGCAGGAGAGGCAGCTAACGACGCAGCCCGTTTCTTATTTCTGTTT
TCTCACAAGCGATGAAAGTGGAAAAGAGGGTGAGCAGGTGGCCCACACATGTGCCTCCAGTGCTGCGGCCCCTCCGGGGACCATCAGCCA
GGCCCCGGGGAGGGAGCCAGCCACAGTGTGTCCGGCTCTTCTCTGAAGGGAAGAGAGCCTTGAATAGACTGAAGCGAAGACGGTTCTGCA
AGGACAAGGCAGACCGAAGGCATTGGTTTTTTTTTTCAGATAAGGAGAATTAGACTCCCAAGTAGACACCAGAGTCACTGTTTGGTTGGT
GGGTGATAGTGGGGTCACAGTGGCTGCCTGTGCTCCCCCAGGGTGAGCGTGACTGTGCTAACCTGGGTGGGGCAGCATGCACACCCCTCT
GGCAGCCCTTTGTTGCTCGCTGATGACAAGTTTGGATGATCCCGCCAAACAGCTTGCTAAGATGTAGTCCCCAGTGTTGGAGGTGGGGCC
TGATGGGAGGTGCTACCCTTGTGAGATAAGGTTGTGTAAAAGCCTGTGGCACCTCCCCACACTGACGCTCTCACCCCTGCTCTGGCCATG
TGCCGCGCCTGCTCCCACTTCCCCTTCTGCCAGGAGTAAAAGCCCCCGAGACCTCCCAGAAGCCAAGCAGATGCTAGTGCCATGCTTCCT

>In-frame_ENST00000583380_ENST00000215832_TCGA-04-1365_SKA2_chr17_57232491_-_MAPK1_chr22_22162135_length(amino acids)=292AA_start in transcript=129_stop in transcript=1007
MREIKILLRFRHENIIGINDIIRAPTIEQMKDVYIVQDLMETDLYKLLKTQHLSNDHICYFLYQILRGLKYIHSANVLHRDLKPSNLLLN
TTCDLKICDFGLARVADPDHDHTGFLTEYVATRWYRAPEIMLNSKGYTKSIDIWSVGCILAEMLSNRPIFPGKHYLDQLNHILGILGSPS
QEDLNCIINLKARNYLLSLPHKNKVPWNRLFPNADSKALDLLDKMLTFNPHKRIEVEQALAHPYLEQYYDPSDEPIAEAPFKFDMELDDL

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Fusion Gene PPI Analysis for SKA2-MAPK1


check button Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in

ChiPPI page.


check button Protein-protein interactors with each fusion partner protein in wild-type (BIOGRID-3.4.160)
HgeneHgene's interactorsTgeneTgene's interactors


check button - Retained PPIs in in-frame fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenStill interaction with


check button - Lost PPIs in in-frame fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenInteraction lost with


check button - Retained PPIs, but lost function due to frame-shift fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenInteraction lost with


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Related Drugs for SKA2-MAPK1


check button Drugs targeting genes involved in this fusion gene.
(DrugBank Version 5.1.8 2021-05-08)
PartnerGeneUniProtAccDrugBank IDDrug nameDrug activityDrug typeDrug status

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Related Diseases for SKA2-MAPK1


check button Diseases associated with fusion partners.
(DisGeNet 4.0)
PartnerGeneDisease IDDisease name# pubmedsSource
TgeneMAPK1C0009171Cocaine Abuse2CTD_human
TgeneMAPK1C0020429Hyperalgesia2CTD_human
TgeneMAPK1C0024121Lung Neoplasms2CTD_human
TgeneMAPK1C0236736Cocaine-Related Disorders2CTD_human
TgeneMAPK1C0242379Malignant neoplasm of lung2CTD_human
TgeneMAPK1C0458247Allodynia2CTD_human
TgeneMAPK1C0600427Cocaine Dependence2CTD_human
TgeneMAPK1C0751211Hyperalgesia, Primary2CTD_human
TgeneMAPK1C0751212Hyperalgesia, Secondary2CTD_human
TgeneMAPK1C0751213Tactile Allodynia2CTD_human
TgeneMAPK1C0751214Hyperalgesia, Thermal2CTD_human
TgeneMAPK1C2936719Mechanical Allodynia2CTD_human
TgeneMAPK1C0005398Cholestasis, Extrahepatic1CTD_human
TgeneMAPK1C0005586Bipolar Disorder1PSYGENET
TgeneMAPK1C0007137Squamous cell carcinoma1CTD_human
TgeneMAPK1C0007786Brain Ischemia1CTD_human
TgeneMAPK1C0017639Gliosis1CTD_human
TgeneMAPK1C0018671Head and Neck Neoplasms1CTD_human
TgeneMAPK1C0018675Head Neoplasms1CTD_human
TgeneMAPK1C0019207Hepatoma, Morris1CTD_human
TgeneMAPK1C0019208Hepatoma, Novikoff1CTD_human
TgeneMAPK1C0020564Hypertrophy1CTD_human
TgeneMAPK1C0021361Female infertility1CTD_human
TgeneMAPK1C0022665Kidney Neoplasm1CTD_human
TgeneMAPK1C0023904Liver Neoplasms, Experimental1CTD_human
TgeneMAPK1C0024623Malignant neoplasm of stomach1CTD_human
TgeneMAPK1C0027533Neck Neoplasms1CTD_human
TgeneMAPK1C0027626Neoplasm Invasiveness1CTD_human
TgeneMAPK1C0027627Neoplasm Metastasis1CTD_human
TgeneMAPK1C0027746Nerve Degeneration1CTD_human
TgeneMAPK1C0033141Cardiomyopathies, Primary1CTD_human
TgeneMAPK1C0034189Pyemia1CTD_human
TgeneMAPK1C0036341Schizophrenia1PSYGENET
TgeneMAPK1C0036529Myocardial Diseases, Secondary1CTD_human
TgeneMAPK1C0036690Septicemia1CTD_human
TgeneMAPK1C0036920Sezary Syndrome1CTD_human
TgeneMAPK1C0038279Sterility, Postpartum1CTD_human
TgeneMAPK1C0038356Stomach Neoplasms1CTD_human
TgeneMAPK1C0038587Substance Withdrawal Syndrome1CTD_human
TgeneMAPK1C0040997Trigeminal Neuralgia1CTD_human
TgeneMAPK1C0086189Drug Withdrawal Symptoms1CTD_human
TgeneMAPK1C0086404Experimental Hepatoma1CTD_human
TgeneMAPK1C0087031Juvenile-Onset Still Disease1CTD_human
TgeneMAPK1C0087169Withdrawal Symptoms1CTD_human
TgeneMAPK1C0155862Streptococcal pneumonia1CTD_human
TgeneMAPK1C0178417Anhedonia1PSYGENET
TgeneMAPK1C0243026Sepsis1CTD_human
TgeneMAPK1C0278996Malignant Head and Neck Neoplasm1CTD_human
TgeneMAPK1C0341869Subfertility, Female1CTD_human
TgeneMAPK1C0393786Trigeminal Neuralgia, Idiopathic1CTD_human
TgeneMAPK1C0393787Secondary Trigeminal Neuralgia1CTD_human
TgeneMAPK1C0740457Malignant neoplasm of kidney1CTD_human
TgeneMAPK1C0746787Cancer of Neck1CTD_human
TgeneMAPK1C0751177Cancer of Head1CTD_human
TgeneMAPK1C0878544Cardiomyopathies1CTD_human
TgeneMAPK1C0887900Upper Aerodigestive Tract Neoplasms1CTD_human
TgeneMAPK1C0917730Female sterility1CTD_human
TgeneMAPK1C0917798Cerebral Ischemia1CTD_human
TgeneMAPK1C0919267ovarian neoplasm1CTD_human
TgeneMAPK1C1140680Malignant neoplasm of ovary1CTD_human
TgeneMAPK1C1708349Hereditary Diffuse Gastric Cancer1CTD_human
TgeneMAPK1C1719672Severe Sepsis1CTD_human
TgeneMAPK1C1866282CEROID LIPOFUSCINOSIS, NEURONAL, 61CTD_human
TgeneMAPK1C3495559Juvenile arthritis1CTD_human
TgeneMAPK1C3714758Juvenile psoriatic arthritis1CTD_human
TgeneMAPK1C3887640Astrocytosis1CTD_human
TgeneMAPK1C4552091Polyarthritis, Juvenile, Rheumatoid Factor Negative1CTD_human
TgeneMAPK1C4704862Polyarthritis, Juvenile, Rheumatoid Factor Positive1CTD_human