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	Fusion Gene Summary
	Fusion Gene ORF analysis
	Fusion Genomic Features
	Fusion Protein Features
	Fusion Gene Sequence
	Fusion Gene PPI analysis
	Related Drugs
	Related Diseases

Fusion gene:TBCD-HLCS (FusionGDB2 ID:88852)

Fusion Gene Summary for TBCD-HLCS

Fusion gene summary

Fusion gene information	Fusion gene name: TBCD-HLCS
	Fusion gene ID: 88852
		Hgene	Tgene
	Gene symbol	TBCD	HLCS
	Gene ID	6904	3141
	Gene name	tubulin folding cofactor D	holocarboxylase synthetase
	Synonyms	PEBAT\|SSD-1\|tfcD	HCS
	Cytomap	17q25.3	21q22.13
	Type of gene	protein-coding	protein-coding
	Description	tubulin-specific chaperone Dbeta-tubulin cofactor D	biotin--protein ligasebiotin apo-protein ligasebiotin--[acetyl-CoA-carboxylase] ligasebiotin--[methylcrotonoyl-CoA-carboxylase] ligasebiotin--[methylmalonyl-CoA-carboxytransferase] ligaseholocarboxylase synthetase (biotin-(proprionyl-CoA-carboxylase
	Modification date	20200313	20200313
	UniProtAcc	.	P50747
	Ensembl transtripts involved in fusion gene	ENST00000355528, ENST00000397466, ENST00000539345, ENST00000576691,	ENST00000336648, ENST00000399120, ENST00000482273,
Fusion gene scores	* DoF score	20 X 23 X 13=5980	15 X 13 X 6=1170
	# samples	27	15
	** MAII score	log2(27/5980*10)=-4.46911417203464 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0	log2(15/1170*10)=-2.96347412397489 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0
Context	PubMed: TBCD [Title/Abstract] AND HLCS [Title/Abstract] AND fusion [Title/Abstract]
Most frequent breakpoint	TBCD(80888519)-HLCS(38139586), # samples:3
Anticipated loss of major functional domain due to fusion event.

* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez

Partner	Gene	GO ID	GO term	PubMed ID
Hgene	TBCD	GO:0006457	protein folding	20740604
Hgene	TBCD	GO:0007021	tubulin complex assembly	28158450
Hgene	TBCD	GO:0007023	post-chaperonin tubulin folding pathway	11847227
Hgene	TBCD	GO:0031115	negative regulation of microtubule polymerization	10831612\|20740604
Tgene	HLCS	GO:0009305	protein biotinylation	7842009
Tgene	HLCS	GO:0016570	histone modification	14613969
Tgene	HLCS	GO:0070781	response to biotin	17904341
Tgene	HLCS	GO:0071110	histone biotinylation	14613969

Fusion gene breakpoints across TBCD (5'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

Fusion gene breakpoints across HLCS (3'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

Fusion gene information from two resources (ChiTars 5.0 and ChimerDB 4.0)
* All genome coordinats were lifted-over on hg19.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.

Source	Disease	Sample	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand
ChimerDB4	SARC	TCGA-HB-A43Z-01A	TBCD	chr17	80888519	+	HLCS	chr21	38139586	-
ChimerDB4	SARC	TCGA-HB-A43Z-01A	TBCD	chr17	80888519	+	HLCS	chr21	38139586	-
ChimerDB4	SARC	TCGA-HB-A43Z-01A	TBCD	chr17	80888519	-	HLCS	chr21	38139586	-

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Fusion Gene ORF analysis for TBCD-HLCS

Open reading frame (ORF) analsis of fusion genes based on Ensembl gene isoform structure.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.

ORF	Henst	Tenst	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand
In-frame	ENST00000355528	ENST00000336648	TBCD	chr17	80888519	+	HLCS	chr21	38139586	-
In-frame	ENST00000355528	ENST00000399120	TBCD	chr17	80888519	+	HLCS	chr21	38139586	-
5CDS-intron	ENST00000355528	ENST00000482273	TBCD	chr17	80888519	+	HLCS	chr21	38139586	-
intron-3CDS	ENST00000397466	ENST00000336648	TBCD	chr17	80888519	+	HLCS	chr21	38139586	-
intron-3CDS	ENST00000397466	ENST00000399120	TBCD	chr17	80888519	+	HLCS	chr21	38139586	-
intron-intron	ENST00000397466	ENST00000482273	TBCD	chr17	80888519	+	HLCS	chr21	38139586	-
In-frame	ENST00000539345	ENST00000336648	TBCD	chr17	80888519	+	HLCS	chr21	38139586	-
In-frame	ENST00000539345	ENST00000399120	TBCD	chr17	80888519	+	HLCS	chr21	38139586	-
5CDS-intron	ENST00000539345	ENST00000482273	TBCD	chr17	80888519	+	HLCS	chr21	38139586	-
intron-3CDS	ENST00000576691	ENST00000336648	TBCD	chr17	80888519	+	HLCS	chr21	38139586	-
intron-3CDS	ENST00000576691	ENST00000399120	TBCD	chr17	80888519	+	HLCS	chr21	38139586	-
intron-intron	ENST00000576691	ENST00000482273	TBCD	chr17	80888519	+	HLCS	chr21	38139586	-

ORFfinder result based on the fusion transcript sequence of in-frame fusion genes.

Henst	Tenst	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	Seq length (transcript)	BP loci (transcript)	Predicted start (transcript)	Predicted stop (transcript)	Seq length (amino acids)
ENST00000355528	TBCD	chr17	80888519	+	ENST00000336648	HLCS	chr21	38139586	-	7331	3243	130	3972	1280
ENST00000355528	TBCD	chr17	80888519	+	ENST00000399120	HLCS	chr21	38139586	-	7027	3243	130	3972	1280
ENST00000539345	TBCD	chr17	80888519	+	ENST00000336648	HLCS	chr21	38139586	-	7237	3149	36	3878	1280
ENST00000539345	TBCD	chr17	80888519	+	ENST00000399120	HLCS	chr21	38139586	-	6933	3149	36	3878	1280

DeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.

Henst	Tenst	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	No-coding score	Coding score
ENST00000355528	ENST00000336648	TBCD	chr17	80888519	+	HLCS	chr21	38139586	-	0.00034772	0.99965227
ENST00000355528	ENST00000399120	TBCD	chr17	80888519	+	HLCS	chr21	38139586	-	0.0004293	0.99957067
ENST00000539345	ENST00000336648	TBCD	chr17	80888519	+	HLCS	chr21	38139586	-	0.000295353	0.99970466
ENST00000539345	ENST00000399120	TBCD	chr17	80888519	+	HLCS	chr21	38139586	-	0.000365364	0.9996346

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Fusion Genomic Features for TBCD-HLCS

FusionAI prediction of the potential fusion gene breakpoint based on the pre-mature RNA sequence context (+/- 5kb of individual partner genes, total 20kb length sequence). FusionAI is a fusion gene breakpoint classifier based on convolutional neural network by comparing the fusion positive and negative sequence context of ~ 20K fusion gene data. From here, we can have the relative potentency of the 20K genomic sequence how individual sequnce will be likely used as the gene fusion breakpoints.

Hgene

Hchr

Hbp

Hstrand

Tgene

Tchr

Tbp

Tstrand

1-p

p (fusion gene breakpoint)

Distribution of 44 human genomic features loci across 20kb length fusion breakpoint regions. We integrated a total of 44 different types of human genomic feature loci information across five big categories including virus integration sites, repeats, structural variants, chromatin states, and gene expression regulation. More details are in help page.

Distribution of 44 human genomic features loci across 20kb length fusion breakpoint regions that are ovelapped with the top 1% feature importance score regions. More details are in help page.

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Fusion Protein Features for TBCD-HLCS

Go to
FGviewer for the breakpoints of chr17:80888519-chr21:38139586
.
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.

Main function of each fusion partner protein. (from UniProt)

Hgene	Tgene
.	HLCS P50747
FUNCTION: Might normally function as a transcriptional repressor. EWS-fusion-proteins (EFPS) may play a role in the tumorigenic process. They may disturb gene expression by mimicking, or interfering with the normal function of CTD-POLII within the transcription initiation complex. They may also contribute to an aberrant activation of the fusion protein target genes.	FUNCTION: Biotin--protein ligase catalyzing the biotinylation of the 4 biotin-dependent carboxylases acetyl-CoA-carboxylase, pyruvate carboxylase, propionyl-CoA carboxylase, and methylcrotonyl-CoA carboxylase. {ECO:0000269\|PubMed:10590022, ECO:0000269\|PubMed:7753853, ECO:0000269\|PubMed:7842009}.

Retention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at
download page

* Minus value of BPloci means that the break pointn is located before the CDS.

- In-frame and retained protein feature among the 13 regional features.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Protein feature

Protein feature note

Hgene

TBCD

chr17:80888519

chr21:38139586

ENST00000355528

361_399

1037.6666666666667

1193.0

Repeat

Note=HEAT 1

Hgene

TBCD

chr17:80888519

chr21:38139586

ENST00000355528

557_594

1037.6666666666667

1193.0

Repeat

Note=HEAT 2

Hgene

TBCD

chr17:80888519

chr21:38139586

ENST00000355528

596_632

1037.6666666666667

1193.0

Repeat

Note=HEAT 3

- In-frame and not-retained protein feature among the 13 regional features.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Protein feature

Protein feature note

Tgene

HLCS

chr17:80888519

chr21:38139586

ENST00000336648

463_652

483.6666666666667

727.0

Domain

BPL/LPL catalytic

Tgene

HLCS

chr17:80888519

chr21:38139586

ENST00000399120

463_652

483.6666666666667

727.0

Domain

BPL/LPL catalytic

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Fusion Gene Sequence for TBCD-HLCS

For in-frame fusion transcripts, we provide the fusion transcript sequences and fusion amino acid sequences. To have fusion amino acid sequence, we ran ORFfinder and chose the longest ORF among the all predicted ones.

>In-frame_ENST00000355528_ENST00000336648_TCGA-HB-A43Z-01A_TBCD_chr17_80888519_+_HLCS_chr21_38139586_length(transcript)=7331nt_BP=3243nt
GGCCAGCGTCGGTTGCCGCCTTAGCGGGCGCCTCCTTTTCATCCCTCATCCTTCATCCCTGGCTTTCGCGCTCTAGCGGAGTGGGATCTG
CGAACACGTGAGGCGGGGGCGCGGTCCCCAGGCTGCCGAGATGGCCCTGAGCGACGAACCGGCCGCGGGCGGCCCCGAGGAGGAGGCGGA
GGACGAGACACTGGCCTTTGGCGCGGCGCTGGAAGCGTTCGGCGAGAGCGCGGAGACCCGGGCGCTGCTGGGCCGCCTGCGGGAGGTGCA
CGGCGGCGGCGCGGAGCGCGAGGTGGCCCTGGAGCGGTTCCGCGTAATAATGGACAAATACCAGGAGCAGCCTCATCTGTTGGACCCGCA
CCTTGAATGGATGATGAACTTGTTGTTGGACATAGTGCAAGATCAGACATCTCCAGCTTCCCTTGTACATCTGGCTTTTAAATTTCTTTA
CATCATCACCAAGGTTCGAGGCTATAAAACATTTCTTCGTTTATTTCCTCATGAAGTTGCCGATGTAGAGCCTGTTTTAGATTTGGTCAC
AATTCAGAATCCCAAGGACCATGAAGCTTGGGAAACCCGCTACATGCTTTTGCTCTGGCTCTCCGTGACCTGCCTGATCCCTTTTGATTT
TTCTCGCCTTGACGGGAACCTCCTCACCCAGCCTGGGCAAGCACGAATGTCCATAATGGACCGTATTCTCCAAATAGCAGAGTCCTACTT
GATTGTCAGTGACAAGGCCCGAGATGCAGCTGCTGTCCTTGTGTCCAGATTTATCACACGTCCTGATGTCAAGCAAAGCAAGATGGCTGA
GTTCCTGGACTGGAGCCTGTGCAATCTGGCCCGTTCCTCCTTCCAGACCATGCAGGGGGTCATCACCATGGATGGGACGCTGCAGGCCCT
GGCACAAATATTTAAACATGGAAAACGTGAAGACTGTTTGCCCTATGCTGCCACTGTCCTCAGGTGCCTCGATGGCTGCAGACTCCCTGA
GAGCAACCAGACCCTGCTGCGGAAGCTGGGGGTGAAGCTTGTGCAGCGACTGGGGCTGACATTCCTGAAGCCGAAGGTGGCAGCATGGAG
GTACCAGCGTGGCTGCCGATCTTTGGCTGCAAATCTGCAGCTCCTCACTCAGGGTCAGAGTGAGCAGAAGCCACTCATCCTGACCGAAGA
TGACGACGAAGATGACGACGTCCCAGAGGGGGTGGAGCGTGTGATAGAGCAGCTGCTGGTCGGGCTGAAGGACAAGGACACGGTCGTGCG
GTGGTCTGCAGCCAAGGGCATCGGTAGGATGGCTGGCAGGCTTCCCAGAGCCCTGGCGGATGATGTGGTCGGGTCTGTGCTGGACTGCTT
CAGTTTCCAGGAGACTGACAAGGCGTGGCATGGGGGATGTCTGGCGCTGGCAGAGCTGGGCAGGAGAGGCCTGTTGCTGCCGTCTCGACT
CGTGGATGTTGTCGCCGTGATCCTGAAGGCGCTGACCTACGACGAGAAGCGGGGTGCCTGCAGCGTGGGCACCAACGTCAGGGACGCCGC
CTGCTACGTGTGCTGGGCCTTCGCGCGTGCCTATGAGCCTCAGGAGCTGAAGCCCTTTGTGACTGCAATCTCGAGTGCACTGGTGATTGC
TGCGGTGTTTGACCGAGACATAAACTGCAGAAGAGCAGCCTCTGCCGCCTTCCAGGAGAATGTGGGGAGACAGGGCACTTTCCCTCATGG
TATTGATATTTTGACCACAGCTGACTATTTTGCCGTCGGTAACAGATCCAACTGTTTCCTGGTTATAAGTGTGTTTATTGCCGGCTTTCC
TGAGTACACGCAGCCAATGATAGACCACCTGGTTACCATGAAGATCAGCCACTGGGATGGGGTCATCCGAGAGTTGGCTGCGAGGGCGCT
GCACAACCTGGCCCAGCAGGCACCCGAGTTCAGCGCCACGCAAGTCTTCCCGAGGCTGCTGTCCATGACACTGAGTCCAGATCTTCACAT
GAGGCATGGGTCGATTCTCGCCTGCGCAGAAGTTGCTTACGCCTTGTACAAACTTGCAGCCCAAGAGAACAGGCCCGTCACGGACCATCT
GGACGAGCAGGCAGTGCAGGGCCTGAAGCAGATTCACCAGCAGCTCTATGATCGTCAGTTATACAGGGGTCTGGGAGGACAGCTCATGAG
ACAAGCAGTGTGTGTTTTAATAGAAAAGTTGTCACTTTCCAAAATGCCCTTTAGAGGTGACACCGTAATTGATGGTTGGCAATGGCTGAT
AAATGACACTTTGAGACATCTCCATCTCATCTCAAGTCACTCCCGCCAGCAGATGAAGGATGCAGCAGTCTCGGCCCTGGCTGCTCTATG
CAGTGAATATTACATGAAGGAGCCGGGGGAGGCAGATCCCGCAATTCAGGAGGAGCTGATCACGCAGTACCTGGCTGAGCTTCGGAACCC
CGAGGAGATGACTCGCTGTGGCTTCTCGTTGGCCTTGGGCGCCCTTCCAGGCTTCCTTCTGAAAGGCCGGCTCCAGCAGGTTCTCACAGG
TTTAAGAGCAGTTACCCACACTTCCCCCGAGGACGTAAGTTTTGCTGAGTCCAGGAGAGACGGCTTGAAGGCCATTGCGAGGATTTGCCA
GACTGTTGGTGTGAAAGCAGGAGCCCCAGACGAAGCTGTGTGCGGAGAGAATGTTTCCCAGATTTACTGTGCGCTGCTGGGCTGCATGGA
CGACTACACCACGGACAGCAGAGGGGACGTGGGCACCTGGGTCCGCAAGGCCGCCATGACCAGTCTGATGGATCTGACACTTCTGCTGGC
TCGGAGCCAGCCTGAGCTGATCGAGGCCCATACCTGTGAGCGCATCATGTGCTGTGTGGCCCAGCAGGCCAGTGAGAAGATTGACCGTTT
CCGTGCTCACGCCGCCAGCGTGTTCCTGACGCTCCTGCACTTTGACAGCCCTCCCATCCCCCACGTGCCCCACCGAGGAGAACTGGAAAA
GCTGTTTCCCAGGTCCGATGTGGCCTCCGTGAACTGGAGTGCACCTTCCCAGGCCTTCCCACGCATCACCCAGCTCCTTGGGCTGCCCAC
CTACCGCTACCACGTCCTGCTGGGGCTAGTCGTGTCCCTGGGCGGCTTGACGGAGTCGACGATCCGGCACTCCACCCAGAGCCTCTTTGA
GTACATGAAGGGCATTCAGAGCGACCCGCAGGCCCTGGGCAGCTTCAGCGGGACCCTTCTGCAGATCTTTGAGGACAACCTTCTGAATGA
GAGGCTGATGTTTCAGACACCGCAGGAAATGGGCTTAATAGTGATCGCGGCCCGGCAGACCGAGGGCAAAGGACGGGGAGGGAATGTGTG
GCTGAGCCCTGTGGGATGTGCTCTTTCTACTCTGCTCATCTCCATTCCACTGAGATCCCAGCTGGGACAGAGGATCCCGTTTGTCCAGCA
TCTGATGTCCGTGGCTGTCGTGGAAGCAGTGAGGTCCATTCCCGAGTATCAGGATATCAACTTACGAGTGAAGTGGCCCAACGATATTTA
TTACAGTGACCTCATGAAGATCGGCGGAGTTCTGGTTAACTCAACACTCATGGGAGAAACATTTTATATACTTATTGGCTGTGGATTTAA
TGTGACTAACAGTAACCCTACCATCTGCATCAACGACCTCATCACAGAATACAATAAACAACACAAGGCAGAACTGAAGCCCTTAAGAGC
CGATTATCTCATCGCCAGAGTCGTGACTGTGCTGGAGAAACTGATCAAAGAGTTTCAGGACAAAGGGCCCAACAGCGTCCTTCCCCTTTA
TTACCGATACTGGGTCCACAGTGGTCAGCAAGTCCATCTGGGCAGCGCAGAGGGACCAAAGGTGTCCATCGTTGGCCTGGACGATTCTGG
CTTCCTCCAGGTTCACCAGGAGGGCGGCGAGGTTGTGACTGTGCACCCGGACGGCAACTCCTTCGACATGCTGAGAAACCTCATCCTCCC
CAAACGGCGGTAATGCCGGGCGTCCCCGAGACGCGGCTGCCTGTCCGTGCCCATGCATCTGGAAATCTAATTTAGAGTTGTAGGTGAATT
TTCTTTTCCTCCAATTCATTTGTTAAGTCTTTGTTCTTTTTCTGTGTTTCTGTTTGTTTTTAGGTTTGTTTTGTTGTCGTTTTCTTTGGT
GTTTGAAGAGGCTCTGGGATAGATGGTTAAGAAGTAGAAAATTTAGTTTAGGGAAAGCCCTCCCACAGGTGGGAAATTGCTCTCCCCTCT
GTGGCTTGGACTTACGTTTATTGTCAAGGGGAGTTTTTACATGGAAATGACAATGGGAAAATTCAGATATTTTCTTAGTAGTGCAGACCT
TTACCCCTAGTCTATGAAAAAACAAACCAAAATATGCTCTTGCGCCCAGGCCAGTGGTGAGTTAGAGGTATGCTATCACTGTTTGTAAGC
ATCTGGGGAGGTACTGAACTGTAAGAACATGCTTGGACACTTAGTCATTGTTCTGTGTTTTTATTAATGAAGAAAAGGGAAGACAGACTT
CCAAGAGTTACTGTCCACCCGGTGGTGTGGCCCCATAGCGAAGTCTAAATGCCTGTAGAGATAGAGCTAGCTGGTGTGGTTGCAGTGACC
TTGTAGAGGAAATCAGTTCATTACTTTGACATCATTCAGTGAGCTCTCCTTTCCTAAGGAAGTTTAAATGTCCTTAGTTAGGGACTGACT
TTCTTAAGTAAGTTTAAATTTACTACATATTGTGAAGAGACAGGATCAAGTTCAGAATCCTTAAATGTCTGATTAGGCATCACTTGGATG
AGGAGGTGGGCGATTTGGCTCTGACAGCTGGAGATGAAGGCACACTCATACCACATACAAGGGAGGATTTGGAGCTTTTAAGCCAGTTTC
AGATTTACTCTGAAATGTGGAGCATTCCTGCAAGACTGTGCAGCTCACGGAATATAGAAGACATGGCATTTTACTCAGAAGTCATAAGTT
TTTGCCCCCCTCATTTACCTCGTATTACCAAGAAAGAAAATGTTATCGATACTAAACACCATCAGTTCAGAGGGAGGATGTGTGTGTGTG
CCCGCATATGTGTGTGCGTGCGTGTGTGCGCACATAGCTTTAAAAGAAGACATTCAAAATTTGATGTGCTACAAGCCTCATGAAAGAACA
AAAGAAATGAAGCCTTTTGATATGCATTCGCTATTCCCAGATGTACGCCATGCCTTTTCCATGTCCCTCCTATCTCTGTTGAACTTATGA
ATCATACTCATTACTTTTCAGCTTTTTAAAAGGCCAATTTTTGTCCAGTTTTCTCTCTTCCAGTCCCAGCTGAAATTAGTGGAAAGAAAG
TTTGATGGAGCTTTCAGCTTTGAACAAAATCCCTTCATTGTAAACTAGCACCATCTTTATCCAGGTCTTACCCAGTCAGGCTAATTCCAG
AAACTTGTGGTTTTTAGTATAGTCTGTCTACCTTTAGCCAGGCACAGGACAGCCCTATGAAAAAATACCCAATATATATTTTTTGGAAAT
GAAACATTAAAAGAACTTAAAAAGTAATTTTTGGAAATGAGGCTTCAATTAGAATTATTTTTCTCAAAAAACAAACAAACAAAAAACACA
AAAAAAACCACTCTTCTCCAAATGCCCAAGCCTTCTTTCAAAATTAGTTAGAAACTTAAGTAAAATACAAGTCCACACCATCCCCAAATT
ACAAAATGGACTTACCCTTGAGAGGGCATCTGCAGAATATCATCAGGGACAAAGATCTCGAGGCTAACGATGTAGGTTTCATTTCTCAGA
CTTTGTAATATAAGGCAAGCCCTCTCTCAGAGCTGCCATCATCACTTTTTGAATTTCTTTGGGGGTTATTTAATGAAAAACATGCTATGT
TTTGTTTTAAGCTGAAGTCCTATTCTGGACACTCTGCTTTGGGAAAAAATGTTATCATTTAATTTCCTTTCTGCAAATTAAAACTAATGA
AGTGTGGCCTTGTCAAAGGCTATGGAGATGTTCCGGGCATACTGCTGTGCTCTGTGCTTTCCAGCAGGCGCTCCTCCCTCACGCAGGAGA
CTCAGTTGTCCTGAGAGAGATGAAGCAGCCTTGAAGCAGATGCTGCGTTTTCCATAAACCTGATTTTGCCTCACATGAACCAAAGACTCT
CAAAACTCCGCTTCTATAGAATTAGCTGAATAAAGGCATTTTACTGATAGCTGTTCGTGTTAGCGAAACCTGTCTACCTGCTATAGCACA
CTCTCCGATTTGGGCCATTTATGCACCCCGCAACCTGGGATCTCAAGGAGCTTTAAAGTCTTAATGGGAACTTGGCATTTTCCTGATGAT
CTTTAAAATGTGGTCACTAAACTCAGGATTGGCGTGTGCTTTTAGAACACTGGAGTAGCCCTTGTTTTAGAGGCTGTGCATTGAGTATCG
ACCGTATTTTGTAAAAGGCAAGATATCCTCCCTTCCAGGCTGGTAACGGGTTTCAAGGGGACTCTTGAGGAAGTGCCCCCTAAAATAGAA
CACAGCAATAACTGGGCTTCCTGTCCCCACCCCCACCCCAGCAGTGCTCTCTGGCACTGGGAACTCTGCTAGGGAGTGGTGGAAGTAGGA
AGGATTTGTGTGCAAAGGAAAATCGTGGTTGAGTTTCACTGCAGCAGGCTGACGTTGCCTGATGTGAGAGCAAGTGGCCGACTGGGGTGC
GGGTGCACAGGTCGGGGGAGCACAGGCCACAGAGCGCAGCCTCTGGGGGTCCCCCAAGGCACAGCATATACAGCATGGTCGCCCCTTGCC
CTGGAGTCTGGGAACAAAGAGAGGAGCCAGCCTCCCCGCACTGCTTCAGATGGAAAAGGGAGGCAGGGTGGGCTTCCGTTCTCCAGATCT
GTTTGCTCTTAACAGGCAGAACATGGGAGAATCCTTATTCCTGGTTAATCACTATGCATATTTGAAATAAAAGAAAGCGTAAGCCTCTGC
AATTTTAACTTCTCAAAGGATGTCTCTGAAAAGAATCACTTTAAACCAATGCCTATAAAAAGCAAGTCTACCAAAATAAACTAAGACTTT
CTATGTGGTTTGGGCTCCCTCTTATTTTTACAAGTTTCATTTTTAAAAGTAGGCAACTACTTTGGGTTACAGTATTTTTATTCATATTTA
AACATTTTTACAAAATAAATAAAGTGTTTTACATAGTAAGGAATATGTACGTATTTCCAAGTATTAAGAAGCCAAGTGTTTTTTTTTTTG
ACGTATTATTGACAAATGTATTCAGCGCCATACACAAGAGAAATATTATTACTCCAAAGAACGAAAGTTAACAAAACTCCAAAGCAAAAA

>In-frame_ENST00000355528_ENST00000336648_TCGA-HB-A43Z-01A_TBCD_chr17_80888519_+_HLCS_chr21_38139586_length(amino acids)=1280AA_start in transcript=130_stop in transcript=3972
MALSDEPAAGGPEEEAEDETLAFGAALEAFGESAETRALLGRLREVHGGGAEREVALERFRVIMDKYQEQPHLLDPHLEWMMNLLLDIVQ
DQTSPASLVHLAFKFLYIITKVRGYKTFLRLFPHEVADVEPVLDLVTIQNPKDHEAWETRYMLLLWLSVTCLIPFDFSRLDGNLLTQPGQ
ARMSIMDRILQIAESYLIVSDKARDAAAVLVSRFITRPDVKQSKMAEFLDWSLCNLARSSFQTMQGVITMDGTLQALAQIFKHGKREDCL
PYAATVLRCLDGCRLPESNQTLLRKLGVKLVQRLGLTFLKPKVAAWRYQRGCRSLAANLQLLTQGQSEQKPLILTEDDDEDDDVPEGVER
VIEQLLVGLKDKDTVVRWSAAKGIGRMAGRLPRALADDVVGSVLDCFSFQETDKAWHGGCLALAELGRRGLLLPSRLVDVVAVILKALTY
DEKRGACSVGTNVRDAACYVCWAFARAYEPQELKPFVTAISSALVIAAVFDRDINCRRAASAAFQENVGRQGTFPHGIDILTTADYFAVG
NRSNCFLVISVFIAGFPEYTQPMIDHLVTMKISHWDGVIRELAARALHNLAQQAPEFSATQVFPRLLSMTLSPDLHMRHGSILACAEVAY
ALYKLAAQENRPVTDHLDEQAVQGLKQIHQQLYDRQLYRGLGGQLMRQAVCVLIEKLSLSKMPFRGDTVIDGWQWLINDTLRHLHLISSH
SRQQMKDAAVSALAALCSEYYMKEPGEADPAIQEELITQYLAELRNPEEMTRCGFSLALGALPGFLLKGRLQQVLTGLRAVTHTSPEDVS
FAESRRDGLKAIARICQTVGVKAGAPDEAVCGENVSQIYCALLGCMDDYTTDSRGDVGTWVRKAAMTSLMDLTLLLARSQPELIEAHTCE
RIMCCVAQQASEKIDRFRAHAASVFLTLLHFDSPPIPHVPHRGELEKLFPRSDVASVNWSAPSQAFPRITQLLGLPTYRYHVLLGLVVSL
GGLTESTIRHSTQSLFEYMKGIQSDPQALGSFSGTLLQIFEDNLLNERLMFQTPQEMGLIVIAARQTEGKGRGGNVWLSPVGCALSTLLI
SIPLRSQLGQRIPFVQHLMSVAVVEAVRSIPEYQDINLRVKWPNDIYYSDLMKIGGVLVNSTLMGETFYILIGCGFNVTNSNPTICINDL
ITEYNKQHKAELKPLRADYLIARVVTVLEKLIKEFQDKGPNSVLPLYYRYWVHSGQQVHLGSAEGPKVSIVGLDDSGFLQVHQEGGEVVT

--------------------------------------------------------------
>In-frame_ENST00000355528_ENST00000399120_TCGA-HB-A43Z-01A_TBCD_chr17_80888519_+_HLCS_chr21_38139586_length(transcript)=7027nt_BP=3243nt
GGCCAGCGTCGGTTGCCGCCTTAGCGGGCGCCTCCTTTTCATCCCTCATCCTTCATCCCTGGCTTTCGCGCTCTAGCGGAGTGGGATCTG
CGAACACGTGAGGCGGGGGCGCGGTCCCCAGGCTGCCGAGATGGCCCTGAGCGACGAACCGGCCGCGGGCGGCCCCGAGGAGGAGGCGGA
GGACGAGACACTGGCCTTTGGCGCGGCGCTGGAAGCGTTCGGCGAGAGCGCGGAGACCCGGGCGCTGCTGGGCCGCCTGCGGGAGGTGCA
CGGCGGCGGCGCGGAGCGCGAGGTGGCCCTGGAGCGGTTCCGCGTAATAATGGACAAATACCAGGAGCAGCCTCATCTGTTGGACCCGCA
CCTTGAATGGATGATGAACTTGTTGTTGGACATAGTGCAAGATCAGACATCTCCAGCTTCCCTTGTACATCTGGCTTTTAAATTTCTTTA
CATCATCACCAAGGTTCGAGGCTATAAAACATTTCTTCGTTTATTTCCTCATGAAGTTGCCGATGTAGAGCCTGTTTTAGATTTGGTCAC
AATTCAGAATCCCAAGGACCATGAAGCTTGGGAAACCCGCTACATGCTTTTGCTCTGGCTCTCCGTGACCTGCCTGATCCCTTTTGATTT
TTCTCGCCTTGACGGGAACCTCCTCACCCAGCCTGGGCAAGCACGAATGTCCATAATGGACCGTATTCTCCAAATAGCAGAGTCCTACTT
GATTGTCAGTGACAAGGCCCGAGATGCAGCTGCTGTCCTTGTGTCCAGATTTATCACACGTCCTGATGTCAAGCAAAGCAAGATGGCTGA
GTTCCTGGACTGGAGCCTGTGCAATCTGGCCCGTTCCTCCTTCCAGACCATGCAGGGGGTCATCACCATGGATGGGACGCTGCAGGCCCT
GGCACAAATATTTAAACATGGAAAACGTGAAGACTGTTTGCCCTATGCTGCCACTGTCCTCAGGTGCCTCGATGGCTGCAGACTCCCTGA
GAGCAACCAGACCCTGCTGCGGAAGCTGGGGGTGAAGCTTGTGCAGCGACTGGGGCTGACATTCCTGAAGCCGAAGGTGGCAGCATGGAG
GTACCAGCGTGGCTGCCGATCTTTGGCTGCAAATCTGCAGCTCCTCACTCAGGGTCAGAGTGAGCAGAAGCCACTCATCCTGACCGAAGA
TGACGACGAAGATGACGACGTCCCAGAGGGGGTGGAGCGTGTGATAGAGCAGCTGCTGGTCGGGCTGAAGGACAAGGACACGGTCGTGCG
GTGGTCTGCAGCCAAGGGCATCGGTAGGATGGCTGGCAGGCTTCCCAGAGCCCTGGCGGATGATGTGGTCGGGTCTGTGCTGGACTGCTT
CAGTTTCCAGGAGACTGACAAGGCGTGGCATGGGGGATGTCTGGCGCTGGCAGAGCTGGGCAGGAGAGGCCTGTTGCTGCCGTCTCGACT
CGTGGATGTTGTCGCCGTGATCCTGAAGGCGCTGACCTACGACGAGAAGCGGGGTGCCTGCAGCGTGGGCACCAACGTCAGGGACGCCGC
CTGCTACGTGTGCTGGGCCTTCGCGCGTGCCTATGAGCCTCAGGAGCTGAAGCCCTTTGTGACTGCAATCTCGAGTGCACTGGTGATTGC
TGCGGTGTTTGACCGAGACATAAACTGCAGAAGAGCAGCCTCTGCCGCCTTCCAGGAGAATGTGGGGAGACAGGGCACTTTCCCTCATGG
TATTGATATTTTGACCACAGCTGACTATTTTGCCGTCGGTAACAGATCCAACTGTTTCCTGGTTATAAGTGTGTTTATTGCCGGCTTTCC
TGAGTACACGCAGCCAATGATAGACCACCTGGTTACCATGAAGATCAGCCACTGGGATGGGGTCATCCGAGAGTTGGCTGCGAGGGCGCT
GCACAACCTGGCCCAGCAGGCACCCGAGTTCAGCGCCACGCAAGTCTTCCCGAGGCTGCTGTCCATGACACTGAGTCCAGATCTTCACAT
GAGGCATGGGTCGATTCTCGCCTGCGCAGAAGTTGCTTACGCCTTGTACAAACTTGCAGCCCAAGAGAACAGGCCCGTCACGGACCATCT
GGACGAGCAGGCAGTGCAGGGCCTGAAGCAGATTCACCAGCAGCTCTATGATCGTCAGTTATACAGGGGTCTGGGAGGACAGCTCATGAG
ACAAGCAGTGTGTGTTTTAATAGAAAAGTTGTCACTTTCCAAAATGCCCTTTAGAGGTGACACCGTAATTGATGGTTGGCAATGGCTGAT
AAATGACACTTTGAGACATCTCCATCTCATCTCAAGTCACTCCCGCCAGCAGATGAAGGATGCAGCAGTCTCGGCCCTGGCTGCTCTATG
CAGTGAATATTACATGAAGGAGCCGGGGGAGGCAGATCCCGCAATTCAGGAGGAGCTGATCACGCAGTACCTGGCTGAGCTTCGGAACCC
CGAGGAGATGACTCGCTGTGGCTTCTCGTTGGCCTTGGGCGCCCTTCCAGGCTTCCTTCTGAAAGGCCGGCTCCAGCAGGTTCTCACAGG
TTTAAGAGCAGTTACCCACACTTCCCCCGAGGACGTAAGTTTTGCTGAGTCCAGGAGAGACGGCTTGAAGGCCATTGCGAGGATTTGCCA
GACTGTTGGTGTGAAAGCAGGAGCCCCAGACGAAGCTGTGTGCGGAGAGAATGTTTCCCAGATTTACTGTGCGCTGCTGGGCTGCATGGA
CGACTACACCACGGACAGCAGAGGGGACGTGGGCACCTGGGTCCGCAAGGCCGCCATGACCAGTCTGATGGATCTGACACTTCTGCTGGC
TCGGAGCCAGCCTGAGCTGATCGAGGCCCATACCTGTGAGCGCATCATGTGCTGTGTGGCCCAGCAGGCCAGTGAGAAGATTGACCGTTT
CCGTGCTCACGCCGCCAGCGTGTTCCTGACGCTCCTGCACTTTGACAGCCCTCCCATCCCCCACGTGCCCCACCGAGGAGAACTGGAAAA
GCTGTTTCCCAGGTCCGATGTGGCCTCCGTGAACTGGAGTGCACCTTCCCAGGCCTTCCCACGCATCACCCAGCTCCTTGGGCTGCCCAC
CTACCGCTACCACGTCCTGCTGGGGCTAGTCGTGTCCCTGGGCGGCTTGACGGAGTCGACGATCCGGCACTCCACCCAGAGCCTCTTTGA
GTACATGAAGGGCATTCAGAGCGACCCGCAGGCCCTGGGCAGCTTCAGCGGGACCCTTCTGCAGATCTTTGAGGACAACCTTCTGAATGA
GAGGCTGATGTTTCAGACACCGCAGGAAATGGGCTTAATAGTGATCGCGGCCCGGCAGACCGAGGGCAAAGGACGGGGAGGGAATGTGTG
GCTGAGCCCTGTGGGATGTGCTCTTTCTACTCTGCTCATCTCCATTCCACTGAGATCCCAGCTGGGACAGAGGATCCCGTTTGTCCAGCA
TCTGATGTCCGTGGCTGTCGTGGAAGCAGTGAGGTCCATTCCCGAGTATCAGGATATCAACTTACGAGTGAAGTGGCCCAACGATATTTA
TTACAGTGACCTCATGAAGATCGGCGGAGTTCTGGTTAACTCAACACTCATGGGAGAAACATTTTATATACTTATTGGCTGTGGATTTAA
TGTGACTAACAGTAACCCTACCATCTGCATCAACGACCTCATCACAGAATACAATAAACAACACAAGGCAGAACTGAAGCCCTTAAGAGC
CGATTATCTCATCGCCAGAGTCGTGACTGTGCTGGAGAAACTGATCAAAGAGTTTCAGGACAAAGGGCCCAACAGCGTCCTTCCCCTTTA
TTACCGATACTGGGTCCACAGTGGTCAGCAAGTCCATCTGGGCAGCGCAGAGGGACCAAAGGTGTCCATCGTTGGCCTGGACGATTCTGG
CTTCCTCCAGGTTCACCAGGAGGGCGGCGAGGTTGTGACTGTGCACCCGGACGGCAACTCCTTCGACATGCTGAGAAACCTCATCCTCCC
CAAACGGCGGTAATGCCGGGCGTCCCCGAGACGCGGCTGCCTGTCCGTGCCCATGCATCTGGAAATCTAATTTAGAGTTGTAGGTGAATT
TTCTTTTCCTCCAATTCATTTGTTAAGTCTTTGTTCTTTTTCTGTGTTTCTGTTTGTTTTTAGGTTTGTTTTGTTGTCGTTTTCTTTGGT
GTTTGAAGAGGCTCTGGGATAGATGGTTAAGAAGTAGAAAATTTAGTTTAGGGAAAGCCCTCCCACAGGTGGGAAATTGCTCTCCCCTCT
GTGGCTTGGACTTACGTTTATTGTCAAGGGGAGTTTTTACATGGAAATGACAATGGGAAAATTCAGATATTTTCTTAGTAGTGCAGACCT
TTACCCCTAGTCTATGAAAAAACAAACCAAAATATGCTCTTGCGCCCAGGCCAGTGGTGAGTTAGAGGTATGCTATCACTGTTTGTAAGC
ATCTGGGGAGGTACTGAACTGTAAGAACATGCTTGGACACTTAGTCATTGTTCTGTGTTTTTATTAATGAAGAAAAGGGAAGACAGACTT
CCAAGAGTTACTGTCCACCCGGTGGTGTGGCCCCATAGCGAAGTCTAAATGCCTGTAGAGATAGAGCTAGCTGGTGTGGTTGCAGTGACC
TTGTAGAGGAAATCAGTTCATTACTTTGACATCATTCAGTGAGCTCTCCTTTCCTAAGGAAGTTTAAATGTCCTTAGTTAGGGACTGACT
TTCTTAAGTAAGTTTAAATTTACTACATATTGTGAAGAGACAGGATCAAGTTCAGAATCCTTAAATGTCTGATTAGGCATCACTTGGATG
AGGAGGTGGGCGATTTGGCTCTGACAGCTGGAGATGAAGGCACACTCATACCACATACAAGGGAGGATTTGGAGCTTTTAAGCCAGTTTC
AGATTTACTCTGAAATGTGGAGCATTCCTGCAAGACTGTGCAGCTCACGGAATATAGAAGACATGGCATTTTACTCAGAAGTCATAAGTT
TTTGCCCCCCTCATTTACCTCGTATTACCAAGAAAGAAAATGTTATCGATACTAAACACCATCAGTTCAGAGGGAGGATGTGTGTGTGTG
CCCGCATATGTGTGTGCGTGCGTGTGTGCGCACATAGCTTTAAAAGAAGACATTCAAAATTTGATGTGCTACAAGCCTCATGAAAGAACA
AAAGAAATGAAGCCTTTTGATATGCATTCGCTATTCCCAGATGTACGCCATGCCTTTTCCATGTCCCTCCTATCTCTGTTGAACTTATGA
ATCATACTCATTACTTTTCAGCTTTTTAAAAGGCCAATTTTTGTCCAGTTTTCTCTCTTCCAGTCCCAGCTGAAATTAGTGGAAAGAAAG
TTTGATGGAGCTTTCAGCTTTGAACAAAATCCCTTCATTGTAAACTAGCACCATCTTTATCCAGGTCTTACCCAGTCAGGCTAATTCCAG
AAACTTGTGGTTTTTAGTATAGTCTGTCTACCTTTAGCCAGGCACAGGACAGCCCTATGAAAAAATACCCAATATATATTTTTTGGAAAT
GAAACATTAAAAGAACTTAAAAAGTAATTTTTGGAAATGAGGCTTCAATTAGAATTATTTTTCTCAAAAAACAAACAAACAAAAAACACA
AAAAAAACCACTCTTCTCCAAATGCCCAAGCCTTCTTTCAAAATTAGTTAGAAACTTAAGTAAAATACAAGTCCACACCATCCCCAAATT
ACAAAATGGACTTACCCTTGAGAGGGCATCTGCAGAATATCATCAGGGACAAAGATCTCGAGGCTAACGATGTAGGTTTCATTTCTCAGA
CTTTGTAATATAAGGCAAGCCCTCTCTCAGAGCTGCCATCATCACTTTTTGAATTTCTTTGGGGGTTATTTAATGAAAAACATGCTATGT
TTTGTTTTAAGCTGAAGTCCTATTCTGGACACTCTGCTTTGGGAAAAAATGTTATCATTTAATTTCCTTTCTGCAAATTAAAACTAATGA
AGTGTGGCCTTGTCAAAGGCTATGGAGATGTTCCGGGCATACTGCTGTGCTCTGTGCTTTCCAGCAGGCGCTCCTCCCTCACGCAGGAGA
CTCAGTTGTCCTGAGAGAGATGAAGCAGCCTTGAAGCAGATGCTGCGTTTTCCATAAACCTGATTTTGCCTCACATGAACCAAAGACTCT
CAAAACTCCGCTTCTATAGAATTAGCTGAATAAAGGCATTTTACTGATAGCTGTTCGTGTTAGCGAAACCTGTCTACCTGCTATAGCACA
CTCTCCGATTTGGGCCATTTATGCACCCCGCAACCTGGGATCTCAAGGAGCTTTAAAGTCTTAATGGGAACTTGGCATTTTCCTGATGAT
CTTTAAAATGTGGTCACTAAACTCAGGATTGGCGTGTGCTTTTAGAACACTGGAGTAGCCCTTGTTTTAGAGGCTGTGCATTGAGTATCG
ACCGTATTTTGTAAAAGGCAAGATATCCTCCCTTCCAGGCTGGTAACGGGTTTCAAGGGGACTCTTGAGGAAGTGCCCCCTAAAATAGAA
CACAGCAATAACTGGGCTTCCTGTCCCCACCCCCACCCCAGCAGTGCTCTCTGGCACTGGGAACTCTGCTAGGGAGTGGTGGAAGTAGGA
AGGATTTGTGTGCAAAGGAAAATCGTGGTTGAGTTTCACTGCAGCAGGCTGACGTTGCCTGATGTGAGAGCAAGTGGCCGACTGGGGTGC
GGGTGCACAGGTCGGGGGAGCACAGGCCACAGAGCGCAGCCTCTGGGGGTCCCCCAAGGCACAGCATATACAGCATGGTCGCCCCTTGCC
CTGGAGTCTGGGAACAAAGAGAGGAGCCAGCCTCCCCGCACTGCTTCAGATGGAAAAGGGAGGCAGGGTGGGCTTCCGTTCTCCAGATCT
GTTTGCTCTTAACAGGCAGAACATGGGAGAATCCTTATTCCTGGTTAATCACTATGCATATTTGAAATAAAAGAAAGCGTAAGCCTCTGC
AATTTTAACTTCTCAAAGGATGTCTCTGAAAAGAATCACTTTAAACCAATGCCTATAAAAAGCAAGTCTACCAAAATAAACTAAGACTTT

>In-frame_ENST00000355528_ENST00000399120_TCGA-HB-A43Z-01A_TBCD_chr17_80888519_+_HLCS_chr21_38139586_length(amino acids)=1280AA_start in transcript=130_stop in transcript=3972
MALSDEPAAGGPEEEAEDETLAFGAALEAFGESAETRALLGRLREVHGGGAEREVALERFRVIMDKYQEQPHLLDPHLEWMMNLLLDIVQ
DQTSPASLVHLAFKFLYIITKVRGYKTFLRLFPHEVADVEPVLDLVTIQNPKDHEAWETRYMLLLWLSVTCLIPFDFSRLDGNLLTQPGQ
ARMSIMDRILQIAESYLIVSDKARDAAAVLVSRFITRPDVKQSKMAEFLDWSLCNLARSSFQTMQGVITMDGTLQALAQIFKHGKREDCL
PYAATVLRCLDGCRLPESNQTLLRKLGVKLVQRLGLTFLKPKVAAWRYQRGCRSLAANLQLLTQGQSEQKPLILTEDDDEDDDVPEGVER
VIEQLLVGLKDKDTVVRWSAAKGIGRMAGRLPRALADDVVGSVLDCFSFQETDKAWHGGCLALAELGRRGLLLPSRLVDVVAVILKALTY
DEKRGACSVGTNVRDAACYVCWAFARAYEPQELKPFVTAISSALVIAAVFDRDINCRRAASAAFQENVGRQGTFPHGIDILTTADYFAVG
NRSNCFLVISVFIAGFPEYTQPMIDHLVTMKISHWDGVIRELAARALHNLAQQAPEFSATQVFPRLLSMTLSPDLHMRHGSILACAEVAY
ALYKLAAQENRPVTDHLDEQAVQGLKQIHQQLYDRQLYRGLGGQLMRQAVCVLIEKLSLSKMPFRGDTVIDGWQWLINDTLRHLHLISSH
SRQQMKDAAVSALAALCSEYYMKEPGEADPAIQEELITQYLAELRNPEEMTRCGFSLALGALPGFLLKGRLQQVLTGLRAVTHTSPEDVS
FAESRRDGLKAIARICQTVGVKAGAPDEAVCGENVSQIYCALLGCMDDYTTDSRGDVGTWVRKAAMTSLMDLTLLLARSQPELIEAHTCE
RIMCCVAQQASEKIDRFRAHAASVFLTLLHFDSPPIPHVPHRGELEKLFPRSDVASVNWSAPSQAFPRITQLLGLPTYRYHVLLGLVVSL
GGLTESTIRHSTQSLFEYMKGIQSDPQALGSFSGTLLQIFEDNLLNERLMFQTPQEMGLIVIAARQTEGKGRGGNVWLSPVGCALSTLLI
SIPLRSQLGQRIPFVQHLMSVAVVEAVRSIPEYQDINLRVKWPNDIYYSDLMKIGGVLVNSTLMGETFYILIGCGFNVTNSNPTICINDL
ITEYNKQHKAELKPLRADYLIARVVTVLEKLIKEFQDKGPNSVLPLYYRYWVHSGQQVHLGSAEGPKVSIVGLDDSGFLQVHQEGGEVVT

--------------------------------------------------------------
>In-frame_ENST00000539345_ENST00000336648_TCGA-HB-A43Z-01A_TBCD_chr17_80888519_+_HLCS_chr21_38139586_length(transcript)=7237nt_BP=3149nt
CACGTGAGGCGGGGGCGCGGTCCCCAGGCTGCCGAGATGGCCCTGAGCGACGAACCGGCCGCGGGCGGCCCCGAGGAGGAGGCGGAGGAC
GAGACACTGGCCTTTGGCGCGGCGCTGGAAGCGTTCGGCGAGAGCGCGGAGACCCGGGCGCTGCTGGGCCGCCTGCGGGAGGTGCACGGC
GGCGGCGCGGAGCGCGAGGTGGCCCTGGAGCGGTTCCGCGTAATAATGGACAAATACCAGGAGCAGCCTCATCTGTTGGACCCGCACCTT
GAATGGATGATGAACTTGTTGTTGGACATAGTGCAAGATCAGACATCTCCAGCTTCCCTTGTACATCTGGCTTTTAAATTTCTTTACATC
ATCACCAAGGTTCGAGGCTATAAAACATTTCTTCGTTTATTTCCTCATGAAGTTGCCGATGTAGAGCCTGTTTTAGATTTGGTCACAATT
CAGAATCCCAAGGACCATGAAGCTTGGGAAACCCGCTACATGCTTTTGCTCTGGCTCTCCGTGACCTGCCTGATCCCTTTTGATTTTTCT
CGCCTTGACGGGAACCTCCTCACCCAGCCTGGGCAAGCACGAATGTCCATAATGGACCGTATTCTCCAAATAGCAGAGTCCTACTTGATT
GTCAGTGACAAGGCCCGAGATGCAGCTGCTGTCCTTGTGTCCAGATTTATCACACGTCCTGATGTCAAGCAAAGCAAGATGGCTGAGTTC
CTGGACTGGAGCCTGTGCAATCTGGCCCGTTCCTCCTTCCAGACCATGCAGGGGGTCATCACCATGGATGGGACGCTGCAGGCCCTGGCA
CAAATATTTAAACATGGAAAACGTGAAGACTGTTTGCCCTATGCTGCCACTGTCCTCAGGTGCCTCGATGGCTGCAGACTCCCTGAGAGC
AACCAGACCCTGCTGCGGAAGCTGGGGGTGAAGCTTGTGCAGCGACTGGGGCTGACATTCCTGAAGCCGAAGGTGGCAGCATGGAGGTAC
CAGCGTGGCTGCCGATCTTTGGCTGCAAATCTGCAGCTCCTCACTCAGGGTCAGAGTGAGCAGAAGCCACTCATCCTGACCGAAGATGAC
GACGAAGATGACGACGTCCCAGAGGGGGTGGAGCGTGTGATAGAGCAGCTGCTGGTCGGGCTGAAGGACAAGGACACGGTCGTGCGGTGG
TCTGCAGCCAAGGGCATCGGTAGGATGGCTGGCAGGCTTCCCAGAGCCCTGGCGGATGATGTGGTCGGGTCTGTGCTGGACTGCTTCAGT
TTCCAGGAGACTGACAAGGCGTGGCATGGGGGATGTCTGGCGCTGGCAGAGCTGGGCAGGAGAGGCCTGTTGCTGCCGTCTCGACTCGTG
GATGTTGTCGCCGTGATCCTGAAGGCGCTGACCTACGACGAGAAGCGGGGTGCCTGCAGCGTGGGCACCAACGTCAGGGACGCCGCCTGC
TACGTGTGCTGGGCCTTCGCGCGTGCCTATGAGCCTCAGGAGCTGAAGCCCTTTGTGACTGCAATCTCGAGTGCACTGGTGATTGCTGCG
GTGTTTGACCGAGACATAAACTGCAGAAGAGCAGCCTCTGCCGCCTTCCAGGAGAATGTGGGGAGACAGGGCACTTTCCCTCATGGTATT
GATATTTTGACCACAGCTGACTATTTTGCCGTCGGTAACAGATCCAACTGTTTCCTGGTTATAAGTGTGTTTATTGCCGGCTTTCCTGAG
TACACGCAGCCAATGATAGACCACCTGGTTACCATGAAGATCAGCCACTGGGATGGGGTCATCCGAGAGTTGGCTGCGAGGGCGCTGCAC
AACCTGGCCCAGCAGGCACCCGAGTTCAGCGCCACGCAAGTCTTCCCGAGGCTGCTGTCCATGACACTGAGTCCAGATCTTCACATGAGG
CATGGGTCGATTCTCGCCTGCGCAGAAGTTGCTTACGCCTTGTACAAACTTGCAGCCCAAGAGAACAGGCCCGTCACGGACCATCTGGAC
GAGCAGGCAGTGCAGGGCCTGAAGCAGATTCACCAGCAGCTCTATGATCGTCAGTTATACAGGGGTCTGGGAGGACAGCTCATGAGACAA
GCAGTGTGTGTTTTAATAGAAAAGTTGTCACTTTCCAAAATGCCCTTTAGAGGTGACACCGTAATTGATGGTTGGCAATGGCTGATAAAT
GACACTTTGAGACATCTCCATCTCATCTCAAGTCACTCCCGCCAGCAGATGAAGGATGCAGCAGTCTCGGCCCTGGCTGCTCTATGCAGT
GAATATTACATGAAGGAGCCGGGGGAGGCAGATCCCGCAATTCAGGAGGAGCTGATCACGCAGTACCTGGCTGAGCTTCGGAACCCCGAG
GAGATGACTCGCTGTGGCTTCTCGTTGGCCTTGGGCGCCCTTCCAGGCTTCCTTCTGAAAGGCCGGCTCCAGCAGGTTCTCACAGGTTTA
AGAGCAGTTACCCACACTTCCCCCGAGGACGTAAGTTTTGCTGAGTCCAGGAGAGACGGCTTGAAGGCCATTGCGAGGATTTGCCAGACT
GTTGGTGTGAAAGCAGGAGCCCCAGACGAAGCTGTGTGCGGAGAGAATGTTTCCCAGATTTACTGTGCGCTGCTGGGCTGCATGGACGAC
TACACCACGGACAGCAGAGGGGACGTGGGCACCTGGGTCCGCAAGGCCGCCATGACCAGTCTGATGGATCTGACACTTCTGCTGGCTCGG
AGCCAGCCTGAGCTGATCGAGGCCCATACCTGTGAGCGCATCATGTGCTGTGTGGCCCAGCAGGCCAGTGAGAAGATTGACCGTTTCCGT
GCTCACGCCGCCAGCGTGTTCCTGACGCTCCTGCACTTTGACAGCCCTCCCATCCCCCACGTGCCCCACCGAGGAGAACTGGAAAAGCTG
TTTCCCAGGTCCGATGTGGCCTCCGTGAACTGGAGTGCACCTTCCCAGGCCTTCCCACGCATCACCCAGCTCCTTGGGCTGCCCACCTAC
CGCTACCACGTCCTGCTGGGGCTAGTCGTGTCCCTGGGCGGCTTGACGGAGTCGACGATCCGGCACTCCACCCAGAGCCTCTTTGAGTAC
ATGAAGGGCATTCAGAGCGACCCGCAGGCCCTGGGCAGCTTCAGCGGGACCCTTCTGCAGATCTTTGAGGACAACCTTCTGAATGAGAGG
CTGATGTTTCAGACACCGCAGGAAATGGGCTTAATAGTGATCGCGGCCCGGCAGACCGAGGGCAAAGGACGGGGAGGGAATGTGTGGCTG
AGCCCTGTGGGATGTGCTCTTTCTACTCTGCTCATCTCCATTCCACTGAGATCCCAGCTGGGACAGAGGATCCCGTTTGTCCAGCATCTG
ATGTCCGTGGCTGTCGTGGAAGCAGTGAGGTCCATTCCCGAGTATCAGGATATCAACTTACGAGTGAAGTGGCCCAACGATATTTATTAC
AGTGACCTCATGAAGATCGGCGGAGTTCTGGTTAACTCAACACTCATGGGAGAAACATTTTATATACTTATTGGCTGTGGATTTAATGTG
ACTAACAGTAACCCTACCATCTGCATCAACGACCTCATCACAGAATACAATAAACAACACAAGGCAGAACTGAAGCCCTTAAGAGCCGAT
TATCTCATCGCCAGAGTCGTGACTGTGCTGGAGAAACTGATCAAAGAGTTTCAGGACAAAGGGCCCAACAGCGTCCTTCCCCTTTATTAC
CGATACTGGGTCCACAGTGGTCAGCAAGTCCATCTGGGCAGCGCAGAGGGACCAAAGGTGTCCATCGTTGGCCTGGACGATTCTGGCTTC
CTCCAGGTTCACCAGGAGGGCGGCGAGGTTGTGACTGTGCACCCGGACGGCAACTCCTTCGACATGCTGAGAAACCTCATCCTCCCCAAA
CGGCGGTAATGCCGGGCGTCCCCGAGACGCGGCTGCCTGTCCGTGCCCATGCATCTGGAAATCTAATTTAGAGTTGTAGGTGAATTTTCT
TTTCCTCCAATTCATTTGTTAAGTCTTTGTTCTTTTTCTGTGTTTCTGTTTGTTTTTAGGTTTGTTTTGTTGTCGTTTTCTTTGGTGTTT
GAAGAGGCTCTGGGATAGATGGTTAAGAAGTAGAAAATTTAGTTTAGGGAAAGCCCTCCCACAGGTGGGAAATTGCTCTCCCCTCTGTGG
CTTGGACTTACGTTTATTGTCAAGGGGAGTTTTTACATGGAAATGACAATGGGAAAATTCAGATATTTTCTTAGTAGTGCAGACCTTTAC
CCCTAGTCTATGAAAAAACAAACCAAAATATGCTCTTGCGCCCAGGCCAGTGGTGAGTTAGAGGTATGCTATCACTGTTTGTAAGCATCT
GGGGAGGTACTGAACTGTAAGAACATGCTTGGACACTTAGTCATTGTTCTGTGTTTTTATTAATGAAGAAAAGGGAAGACAGACTTCCAA
GAGTTACTGTCCACCCGGTGGTGTGGCCCCATAGCGAAGTCTAAATGCCTGTAGAGATAGAGCTAGCTGGTGTGGTTGCAGTGACCTTGT
AGAGGAAATCAGTTCATTACTTTGACATCATTCAGTGAGCTCTCCTTTCCTAAGGAAGTTTAAATGTCCTTAGTTAGGGACTGACTTTCT
TAAGTAAGTTTAAATTTACTACATATTGTGAAGAGACAGGATCAAGTTCAGAATCCTTAAATGTCTGATTAGGCATCACTTGGATGAGGA
GGTGGGCGATTTGGCTCTGACAGCTGGAGATGAAGGCACACTCATACCACATACAAGGGAGGATTTGGAGCTTTTAAGCCAGTTTCAGAT
TTACTCTGAAATGTGGAGCATTCCTGCAAGACTGTGCAGCTCACGGAATATAGAAGACATGGCATTTTACTCAGAAGTCATAAGTTTTTG
CCCCCCTCATTTACCTCGTATTACCAAGAAAGAAAATGTTATCGATACTAAACACCATCAGTTCAGAGGGAGGATGTGTGTGTGTGCCCG
CATATGTGTGTGCGTGCGTGTGTGCGCACATAGCTTTAAAAGAAGACATTCAAAATTTGATGTGCTACAAGCCTCATGAAAGAACAAAAG
AAATGAAGCCTTTTGATATGCATTCGCTATTCCCAGATGTACGCCATGCCTTTTCCATGTCCCTCCTATCTCTGTTGAACTTATGAATCA
TACTCATTACTTTTCAGCTTTTTAAAAGGCCAATTTTTGTCCAGTTTTCTCTCTTCCAGTCCCAGCTGAAATTAGTGGAAAGAAAGTTTG
ATGGAGCTTTCAGCTTTGAACAAAATCCCTTCATTGTAAACTAGCACCATCTTTATCCAGGTCTTACCCAGTCAGGCTAATTCCAGAAAC
TTGTGGTTTTTAGTATAGTCTGTCTACCTTTAGCCAGGCACAGGACAGCCCTATGAAAAAATACCCAATATATATTTTTTGGAAATGAAA
CATTAAAAGAACTTAAAAAGTAATTTTTGGAAATGAGGCTTCAATTAGAATTATTTTTCTCAAAAAACAAACAAACAAAAAACACAAAAA
AAACCACTCTTCTCCAAATGCCCAAGCCTTCTTTCAAAATTAGTTAGAAACTTAAGTAAAATACAAGTCCACACCATCCCCAAATTACAA
AATGGACTTACCCTTGAGAGGGCATCTGCAGAATATCATCAGGGACAAAGATCTCGAGGCTAACGATGTAGGTTTCATTTCTCAGACTTT
GTAATATAAGGCAAGCCCTCTCTCAGAGCTGCCATCATCACTTTTTGAATTTCTTTGGGGGTTATTTAATGAAAAACATGCTATGTTTTG
TTTTAAGCTGAAGTCCTATTCTGGACACTCTGCTTTGGGAAAAAATGTTATCATTTAATTTCCTTTCTGCAAATTAAAACTAATGAAGTG
TGGCCTTGTCAAAGGCTATGGAGATGTTCCGGGCATACTGCTGTGCTCTGTGCTTTCCAGCAGGCGCTCCTCCCTCACGCAGGAGACTCA
GTTGTCCTGAGAGAGATGAAGCAGCCTTGAAGCAGATGCTGCGTTTTCCATAAACCTGATTTTGCCTCACATGAACCAAAGACTCTCAAA
ACTCCGCTTCTATAGAATTAGCTGAATAAAGGCATTTTACTGATAGCTGTTCGTGTTAGCGAAACCTGTCTACCTGCTATAGCACACTCT
CCGATTTGGGCCATTTATGCACCCCGCAACCTGGGATCTCAAGGAGCTTTAAAGTCTTAATGGGAACTTGGCATTTTCCTGATGATCTTT
AAAATGTGGTCACTAAACTCAGGATTGGCGTGTGCTTTTAGAACACTGGAGTAGCCCTTGTTTTAGAGGCTGTGCATTGAGTATCGACCG
TATTTTGTAAAAGGCAAGATATCCTCCCTTCCAGGCTGGTAACGGGTTTCAAGGGGACTCTTGAGGAAGTGCCCCCTAAAATAGAACACA
GCAATAACTGGGCTTCCTGTCCCCACCCCCACCCCAGCAGTGCTCTCTGGCACTGGGAACTCTGCTAGGGAGTGGTGGAAGTAGGAAGGA
TTTGTGTGCAAAGGAAAATCGTGGTTGAGTTTCACTGCAGCAGGCTGACGTTGCCTGATGTGAGAGCAAGTGGCCGACTGGGGTGCGGGT
GCACAGGTCGGGGGAGCACAGGCCACAGAGCGCAGCCTCTGGGGGTCCCCCAAGGCACAGCATATACAGCATGGTCGCCCCTTGCCCTGG
AGTCTGGGAACAAAGAGAGGAGCCAGCCTCCCCGCACTGCTTCAGATGGAAAAGGGAGGCAGGGTGGGCTTCCGTTCTCCAGATCTGTTT
GCTCTTAACAGGCAGAACATGGGAGAATCCTTATTCCTGGTTAATCACTATGCATATTTGAAATAAAAGAAAGCGTAAGCCTCTGCAATT
TTAACTTCTCAAAGGATGTCTCTGAAAAGAATCACTTTAAACCAATGCCTATAAAAAGCAAGTCTACCAAAATAAACTAAGACTTTCTAT
GTGGTTTGGGCTCCCTCTTATTTTTACAAGTTTCATTTTTAAAAGTAGGCAACTACTTTGGGTTACAGTATTTTTATTCATATTTAAACA
TTTTTACAAAATAAATAAAGTGTTTTACATAGTAAGGAATATGTACGTATTTCCAAGTATTAAGAAGCCAAGTGTTTTTTTTTTTGACGT
ATTATTGACAAATGTATTCAGCGCCATACACAAGAGAAATATTATTACTCCAAAGAACGAAAGTTAACAAAACTCCAAAGCAAAAACCCT

>In-frame_ENST00000539345_ENST00000336648_TCGA-HB-A43Z-01A_TBCD_chr17_80888519_+_HLCS_chr21_38139586_length(amino acids)=1280AA_start in transcript=36_stop in transcript=3878
MALSDEPAAGGPEEEAEDETLAFGAALEAFGESAETRALLGRLREVHGGGAEREVALERFRVIMDKYQEQPHLLDPHLEWMMNLLLDIVQ
DQTSPASLVHLAFKFLYIITKVRGYKTFLRLFPHEVADVEPVLDLVTIQNPKDHEAWETRYMLLLWLSVTCLIPFDFSRLDGNLLTQPGQ
ARMSIMDRILQIAESYLIVSDKARDAAAVLVSRFITRPDVKQSKMAEFLDWSLCNLARSSFQTMQGVITMDGTLQALAQIFKHGKREDCL
PYAATVLRCLDGCRLPESNQTLLRKLGVKLVQRLGLTFLKPKVAAWRYQRGCRSLAANLQLLTQGQSEQKPLILTEDDDEDDDVPEGVER
VIEQLLVGLKDKDTVVRWSAAKGIGRMAGRLPRALADDVVGSVLDCFSFQETDKAWHGGCLALAELGRRGLLLPSRLVDVVAVILKALTY
DEKRGACSVGTNVRDAACYVCWAFARAYEPQELKPFVTAISSALVIAAVFDRDINCRRAASAAFQENVGRQGTFPHGIDILTTADYFAVG
NRSNCFLVISVFIAGFPEYTQPMIDHLVTMKISHWDGVIRELAARALHNLAQQAPEFSATQVFPRLLSMTLSPDLHMRHGSILACAEVAY
ALYKLAAQENRPVTDHLDEQAVQGLKQIHQQLYDRQLYRGLGGQLMRQAVCVLIEKLSLSKMPFRGDTVIDGWQWLINDTLRHLHLISSH
SRQQMKDAAVSALAALCSEYYMKEPGEADPAIQEELITQYLAELRNPEEMTRCGFSLALGALPGFLLKGRLQQVLTGLRAVTHTSPEDVS
FAESRRDGLKAIARICQTVGVKAGAPDEAVCGENVSQIYCALLGCMDDYTTDSRGDVGTWVRKAAMTSLMDLTLLLARSQPELIEAHTCE
RIMCCVAQQASEKIDRFRAHAASVFLTLLHFDSPPIPHVPHRGELEKLFPRSDVASVNWSAPSQAFPRITQLLGLPTYRYHVLLGLVVSL
GGLTESTIRHSTQSLFEYMKGIQSDPQALGSFSGTLLQIFEDNLLNERLMFQTPQEMGLIVIAARQTEGKGRGGNVWLSPVGCALSTLLI
SIPLRSQLGQRIPFVQHLMSVAVVEAVRSIPEYQDINLRVKWPNDIYYSDLMKIGGVLVNSTLMGETFYILIGCGFNVTNSNPTICINDL
ITEYNKQHKAELKPLRADYLIARVVTVLEKLIKEFQDKGPNSVLPLYYRYWVHSGQQVHLGSAEGPKVSIVGLDDSGFLQVHQEGGEVVT

--------------------------------------------------------------
>In-frame_ENST00000539345_ENST00000399120_TCGA-HB-A43Z-01A_TBCD_chr17_80888519_+_HLCS_chr21_38139586_length(transcript)=6933nt_BP=3149nt
CACGTGAGGCGGGGGCGCGGTCCCCAGGCTGCCGAGATGGCCCTGAGCGACGAACCGGCCGCGGGCGGCCCCGAGGAGGAGGCGGAGGAC
GAGACACTGGCCTTTGGCGCGGCGCTGGAAGCGTTCGGCGAGAGCGCGGAGACCCGGGCGCTGCTGGGCCGCCTGCGGGAGGTGCACGGC
GGCGGCGCGGAGCGCGAGGTGGCCCTGGAGCGGTTCCGCGTAATAATGGACAAATACCAGGAGCAGCCTCATCTGTTGGACCCGCACCTT
GAATGGATGATGAACTTGTTGTTGGACATAGTGCAAGATCAGACATCTCCAGCTTCCCTTGTACATCTGGCTTTTAAATTTCTTTACATC
ATCACCAAGGTTCGAGGCTATAAAACATTTCTTCGTTTATTTCCTCATGAAGTTGCCGATGTAGAGCCTGTTTTAGATTTGGTCACAATT
CAGAATCCCAAGGACCATGAAGCTTGGGAAACCCGCTACATGCTTTTGCTCTGGCTCTCCGTGACCTGCCTGATCCCTTTTGATTTTTCT
CGCCTTGACGGGAACCTCCTCACCCAGCCTGGGCAAGCACGAATGTCCATAATGGACCGTATTCTCCAAATAGCAGAGTCCTACTTGATT
GTCAGTGACAAGGCCCGAGATGCAGCTGCTGTCCTTGTGTCCAGATTTATCACACGTCCTGATGTCAAGCAAAGCAAGATGGCTGAGTTC
CTGGACTGGAGCCTGTGCAATCTGGCCCGTTCCTCCTTCCAGACCATGCAGGGGGTCATCACCATGGATGGGACGCTGCAGGCCCTGGCA
CAAATATTTAAACATGGAAAACGTGAAGACTGTTTGCCCTATGCTGCCACTGTCCTCAGGTGCCTCGATGGCTGCAGACTCCCTGAGAGC
AACCAGACCCTGCTGCGGAAGCTGGGGGTGAAGCTTGTGCAGCGACTGGGGCTGACATTCCTGAAGCCGAAGGTGGCAGCATGGAGGTAC
CAGCGTGGCTGCCGATCTTTGGCTGCAAATCTGCAGCTCCTCACTCAGGGTCAGAGTGAGCAGAAGCCACTCATCCTGACCGAAGATGAC
GACGAAGATGACGACGTCCCAGAGGGGGTGGAGCGTGTGATAGAGCAGCTGCTGGTCGGGCTGAAGGACAAGGACACGGTCGTGCGGTGG
TCTGCAGCCAAGGGCATCGGTAGGATGGCTGGCAGGCTTCCCAGAGCCCTGGCGGATGATGTGGTCGGGTCTGTGCTGGACTGCTTCAGT
TTCCAGGAGACTGACAAGGCGTGGCATGGGGGATGTCTGGCGCTGGCAGAGCTGGGCAGGAGAGGCCTGTTGCTGCCGTCTCGACTCGTG
GATGTTGTCGCCGTGATCCTGAAGGCGCTGACCTACGACGAGAAGCGGGGTGCCTGCAGCGTGGGCACCAACGTCAGGGACGCCGCCTGC
TACGTGTGCTGGGCCTTCGCGCGTGCCTATGAGCCTCAGGAGCTGAAGCCCTTTGTGACTGCAATCTCGAGTGCACTGGTGATTGCTGCG
GTGTTTGACCGAGACATAAACTGCAGAAGAGCAGCCTCTGCCGCCTTCCAGGAGAATGTGGGGAGACAGGGCACTTTCCCTCATGGTATT
GATATTTTGACCACAGCTGACTATTTTGCCGTCGGTAACAGATCCAACTGTTTCCTGGTTATAAGTGTGTTTATTGCCGGCTTTCCTGAG
TACACGCAGCCAATGATAGACCACCTGGTTACCATGAAGATCAGCCACTGGGATGGGGTCATCCGAGAGTTGGCTGCGAGGGCGCTGCAC
AACCTGGCCCAGCAGGCACCCGAGTTCAGCGCCACGCAAGTCTTCCCGAGGCTGCTGTCCATGACACTGAGTCCAGATCTTCACATGAGG
CATGGGTCGATTCTCGCCTGCGCAGAAGTTGCTTACGCCTTGTACAAACTTGCAGCCCAAGAGAACAGGCCCGTCACGGACCATCTGGAC
GAGCAGGCAGTGCAGGGCCTGAAGCAGATTCACCAGCAGCTCTATGATCGTCAGTTATACAGGGGTCTGGGAGGACAGCTCATGAGACAA
GCAGTGTGTGTTTTAATAGAAAAGTTGTCACTTTCCAAAATGCCCTTTAGAGGTGACACCGTAATTGATGGTTGGCAATGGCTGATAAAT
GACACTTTGAGACATCTCCATCTCATCTCAAGTCACTCCCGCCAGCAGATGAAGGATGCAGCAGTCTCGGCCCTGGCTGCTCTATGCAGT
GAATATTACATGAAGGAGCCGGGGGAGGCAGATCCCGCAATTCAGGAGGAGCTGATCACGCAGTACCTGGCTGAGCTTCGGAACCCCGAG
GAGATGACTCGCTGTGGCTTCTCGTTGGCCTTGGGCGCCCTTCCAGGCTTCCTTCTGAAAGGCCGGCTCCAGCAGGTTCTCACAGGTTTA
AGAGCAGTTACCCACACTTCCCCCGAGGACGTAAGTTTTGCTGAGTCCAGGAGAGACGGCTTGAAGGCCATTGCGAGGATTTGCCAGACT
GTTGGTGTGAAAGCAGGAGCCCCAGACGAAGCTGTGTGCGGAGAGAATGTTTCCCAGATTTACTGTGCGCTGCTGGGCTGCATGGACGAC
TACACCACGGACAGCAGAGGGGACGTGGGCACCTGGGTCCGCAAGGCCGCCATGACCAGTCTGATGGATCTGACACTTCTGCTGGCTCGG
AGCCAGCCTGAGCTGATCGAGGCCCATACCTGTGAGCGCATCATGTGCTGTGTGGCCCAGCAGGCCAGTGAGAAGATTGACCGTTTCCGT
GCTCACGCCGCCAGCGTGTTCCTGACGCTCCTGCACTTTGACAGCCCTCCCATCCCCCACGTGCCCCACCGAGGAGAACTGGAAAAGCTG
TTTCCCAGGTCCGATGTGGCCTCCGTGAACTGGAGTGCACCTTCCCAGGCCTTCCCACGCATCACCCAGCTCCTTGGGCTGCCCACCTAC
CGCTACCACGTCCTGCTGGGGCTAGTCGTGTCCCTGGGCGGCTTGACGGAGTCGACGATCCGGCACTCCACCCAGAGCCTCTTTGAGTAC
ATGAAGGGCATTCAGAGCGACCCGCAGGCCCTGGGCAGCTTCAGCGGGACCCTTCTGCAGATCTTTGAGGACAACCTTCTGAATGAGAGG
CTGATGTTTCAGACACCGCAGGAAATGGGCTTAATAGTGATCGCGGCCCGGCAGACCGAGGGCAAAGGACGGGGAGGGAATGTGTGGCTG
AGCCCTGTGGGATGTGCTCTTTCTACTCTGCTCATCTCCATTCCACTGAGATCCCAGCTGGGACAGAGGATCCCGTTTGTCCAGCATCTG
ATGTCCGTGGCTGTCGTGGAAGCAGTGAGGTCCATTCCCGAGTATCAGGATATCAACTTACGAGTGAAGTGGCCCAACGATATTTATTAC
AGTGACCTCATGAAGATCGGCGGAGTTCTGGTTAACTCAACACTCATGGGAGAAACATTTTATATACTTATTGGCTGTGGATTTAATGTG
ACTAACAGTAACCCTACCATCTGCATCAACGACCTCATCACAGAATACAATAAACAACACAAGGCAGAACTGAAGCCCTTAAGAGCCGAT
TATCTCATCGCCAGAGTCGTGACTGTGCTGGAGAAACTGATCAAAGAGTTTCAGGACAAAGGGCCCAACAGCGTCCTTCCCCTTTATTAC
CGATACTGGGTCCACAGTGGTCAGCAAGTCCATCTGGGCAGCGCAGAGGGACCAAAGGTGTCCATCGTTGGCCTGGACGATTCTGGCTTC
CTCCAGGTTCACCAGGAGGGCGGCGAGGTTGTGACTGTGCACCCGGACGGCAACTCCTTCGACATGCTGAGAAACCTCATCCTCCCCAAA
CGGCGGTAATGCCGGGCGTCCCCGAGACGCGGCTGCCTGTCCGTGCCCATGCATCTGGAAATCTAATTTAGAGTTGTAGGTGAATTTTCT
TTTCCTCCAATTCATTTGTTAAGTCTTTGTTCTTTTTCTGTGTTTCTGTTTGTTTTTAGGTTTGTTTTGTTGTCGTTTTCTTTGGTGTTT
GAAGAGGCTCTGGGATAGATGGTTAAGAAGTAGAAAATTTAGTTTAGGGAAAGCCCTCCCACAGGTGGGAAATTGCTCTCCCCTCTGTGG
CTTGGACTTACGTTTATTGTCAAGGGGAGTTTTTACATGGAAATGACAATGGGAAAATTCAGATATTTTCTTAGTAGTGCAGACCTTTAC
CCCTAGTCTATGAAAAAACAAACCAAAATATGCTCTTGCGCCCAGGCCAGTGGTGAGTTAGAGGTATGCTATCACTGTTTGTAAGCATCT
GGGGAGGTACTGAACTGTAAGAACATGCTTGGACACTTAGTCATTGTTCTGTGTTTTTATTAATGAAGAAAAGGGAAGACAGACTTCCAA
GAGTTACTGTCCACCCGGTGGTGTGGCCCCATAGCGAAGTCTAAATGCCTGTAGAGATAGAGCTAGCTGGTGTGGTTGCAGTGACCTTGT
AGAGGAAATCAGTTCATTACTTTGACATCATTCAGTGAGCTCTCCTTTCCTAAGGAAGTTTAAATGTCCTTAGTTAGGGACTGACTTTCT
TAAGTAAGTTTAAATTTACTACATATTGTGAAGAGACAGGATCAAGTTCAGAATCCTTAAATGTCTGATTAGGCATCACTTGGATGAGGA
GGTGGGCGATTTGGCTCTGACAGCTGGAGATGAAGGCACACTCATACCACATACAAGGGAGGATTTGGAGCTTTTAAGCCAGTTTCAGAT
TTACTCTGAAATGTGGAGCATTCCTGCAAGACTGTGCAGCTCACGGAATATAGAAGACATGGCATTTTACTCAGAAGTCATAAGTTTTTG
CCCCCCTCATTTACCTCGTATTACCAAGAAAGAAAATGTTATCGATACTAAACACCATCAGTTCAGAGGGAGGATGTGTGTGTGTGCCCG
CATATGTGTGTGCGTGCGTGTGTGCGCACATAGCTTTAAAAGAAGACATTCAAAATTTGATGTGCTACAAGCCTCATGAAAGAACAAAAG
AAATGAAGCCTTTTGATATGCATTCGCTATTCCCAGATGTACGCCATGCCTTTTCCATGTCCCTCCTATCTCTGTTGAACTTATGAATCA
TACTCATTACTTTTCAGCTTTTTAAAAGGCCAATTTTTGTCCAGTTTTCTCTCTTCCAGTCCCAGCTGAAATTAGTGGAAAGAAAGTTTG
ATGGAGCTTTCAGCTTTGAACAAAATCCCTTCATTGTAAACTAGCACCATCTTTATCCAGGTCTTACCCAGTCAGGCTAATTCCAGAAAC
TTGTGGTTTTTAGTATAGTCTGTCTACCTTTAGCCAGGCACAGGACAGCCCTATGAAAAAATACCCAATATATATTTTTTGGAAATGAAA
CATTAAAAGAACTTAAAAAGTAATTTTTGGAAATGAGGCTTCAATTAGAATTATTTTTCTCAAAAAACAAACAAACAAAAAACACAAAAA
AAACCACTCTTCTCCAAATGCCCAAGCCTTCTTTCAAAATTAGTTAGAAACTTAAGTAAAATACAAGTCCACACCATCCCCAAATTACAA
AATGGACTTACCCTTGAGAGGGCATCTGCAGAATATCATCAGGGACAAAGATCTCGAGGCTAACGATGTAGGTTTCATTTCTCAGACTTT
GTAATATAAGGCAAGCCCTCTCTCAGAGCTGCCATCATCACTTTTTGAATTTCTTTGGGGGTTATTTAATGAAAAACATGCTATGTTTTG
TTTTAAGCTGAAGTCCTATTCTGGACACTCTGCTTTGGGAAAAAATGTTATCATTTAATTTCCTTTCTGCAAATTAAAACTAATGAAGTG
TGGCCTTGTCAAAGGCTATGGAGATGTTCCGGGCATACTGCTGTGCTCTGTGCTTTCCAGCAGGCGCTCCTCCCTCACGCAGGAGACTCA
GTTGTCCTGAGAGAGATGAAGCAGCCTTGAAGCAGATGCTGCGTTTTCCATAAACCTGATTTTGCCTCACATGAACCAAAGACTCTCAAA
ACTCCGCTTCTATAGAATTAGCTGAATAAAGGCATTTTACTGATAGCTGTTCGTGTTAGCGAAACCTGTCTACCTGCTATAGCACACTCT
CCGATTTGGGCCATTTATGCACCCCGCAACCTGGGATCTCAAGGAGCTTTAAAGTCTTAATGGGAACTTGGCATTTTCCTGATGATCTTT
AAAATGTGGTCACTAAACTCAGGATTGGCGTGTGCTTTTAGAACACTGGAGTAGCCCTTGTTTTAGAGGCTGTGCATTGAGTATCGACCG
TATTTTGTAAAAGGCAAGATATCCTCCCTTCCAGGCTGGTAACGGGTTTCAAGGGGACTCTTGAGGAAGTGCCCCCTAAAATAGAACACA
GCAATAACTGGGCTTCCTGTCCCCACCCCCACCCCAGCAGTGCTCTCTGGCACTGGGAACTCTGCTAGGGAGTGGTGGAAGTAGGAAGGA
TTTGTGTGCAAAGGAAAATCGTGGTTGAGTTTCACTGCAGCAGGCTGACGTTGCCTGATGTGAGAGCAAGTGGCCGACTGGGGTGCGGGT
GCACAGGTCGGGGGAGCACAGGCCACAGAGCGCAGCCTCTGGGGGTCCCCCAAGGCACAGCATATACAGCATGGTCGCCCCTTGCCCTGG
AGTCTGGGAACAAAGAGAGGAGCCAGCCTCCCCGCACTGCTTCAGATGGAAAAGGGAGGCAGGGTGGGCTTCCGTTCTCCAGATCTGTTT
GCTCTTAACAGGCAGAACATGGGAGAATCCTTATTCCTGGTTAATCACTATGCATATTTGAAATAAAAGAAAGCGTAAGCCTCTGCAATT
TTAACTTCTCAAAGGATGTCTCTGAAAAGAATCACTTTAAACCAATGCCTATAAAAAGCAAGTCTACCAAAATAAACTAAGACTTTCTAT

>In-frame_ENST00000539345_ENST00000399120_TCGA-HB-A43Z-01A_TBCD_chr17_80888519_+_HLCS_chr21_38139586_length(amino acids)=1280AA_start in transcript=36_stop in transcript=3878
MALSDEPAAGGPEEEAEDETLAFGAALEAFGESAETRALLGRLREVHGGGAEREVALERFRVIMDKYQEQPHLLDPHLEWMMNLLLDIVQ
DQTSPASLVHLAFKFLYIITKVRGYKTFLRLFPHEVADVEPVLDLVTIQNPKDHEAWETRYMLLLWLSVTCLIPFDFSRLDGNLLTQPGQ
ARMSIMDRILQIAESYLIVSDKARDAAAVLVSRFITRPDVKQSKMAEFLDWSLCNLARSSFQTMQGVITMDGTLQALAQIFKHGKREDCL
PYAATVLRCLDGCRLPESNQTLLRKLGVKLVQRLGLTFLKPKVAAWRYQRGCRSLAANLQLLTQGQSEQKPLILTEDDDEDDDVPEGVER
VIEQLLVGLKDKDTVVRWSAAKGIGRMAGRLPRALADDVVGSVLDCFSFQETDKAWHGGCLALAELGRRGLLLPSRLVDVVAVILKALTY
DEKRGACSVGTNVRDAACYVCWAFARAYEPQELKPFVTAISSALVIAAVFDRDINCRRAASAAFQENVGRQGTFPHGIDILTTADYFAVG
NRSNCFLVISVFIAGFPEYTQPMIDHLVTMKISHWDGVIRELAARALHNLAQQAPEFSATQVFPRLLSMTLSPDLHMRHGSILACAEVAY
ALYKLAAQENRPVTDHLDEQAVQGLKQIHQQLYDRQLYRGLGGQLMRQAVCVLIEKLSLSKMPFRGDTVIDGWQWLINDTLRHLHLISSH
SRQQMKDAAVSALAALCSEYYMKEPGEADPAIQEELITQYLAELRNPEEMTRCGFSLALGALPGFLLKGRLQQVLTGLRAVTHTSPEDVS
FAESRRDGLKAIARICQTVGVKAGAPDEAVCGENVSQIYCALLGCMDDYTTDSRGDVGTWVRKAAMTSLMDLTLLLARSQPELIEAHTCE
RIMCCVAQQASEKIDRFRAHAASVFLTLLHFDSPPIPHVPHRGELEKLFPRSDVASVNWSAPSQAFPRITQLLGLPTYRYHVLLGLVVSL
GGLTESTIRHSTQSLFEYMKGIQSDPQALGSFSGTLLQIFEDNLLNERLMFQTPQEMGLIVIAARQTEGKGRGGNVWLSPVGCALSTLLI
SIPLRSQLGQRIPFVQHLMSVAVVEAVRSIPEYQDINLRVKWPNDIYYSDLMKIGGVLVNSTLMGETFYILIGCGFNVTNSNPTICINDL
ITEYNKQHKAELKPLRADYLIARVVTVLEKLIKEFQDKGPNSVLPLYYRYWVHSGQQVHLGSAEGPKVSIVGLDDSGFLQVHQEGGEVVT

--------------------------------------------------------------

Top

Fusion Gene PPI Analysis for TBCD-HLCS

Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in
ChiPPI page.

Protein-protein interactors with each fusion partner protein in wild-type (BIOGRID-3.4.160)

Hgene

Hgene's interactors

Tgene

Tgene's interactors

- Retained PPIs in in-frame fusion.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Still interaction with

- Lost PPIs in in-frame fusion.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Interaction lost with

- Retained PPIs, but lost function due to frame-shift fusion.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Interaction lost with

Top

Related Drugs for TBCD-HLCS

Drugs targeting genes involved in this fusion gene.
(DrugBank Version 5.1.8 2021-05-08)

Partner	Gene	UniProtAcc	DrugBank ID	Drug name	Drug activity	Drug type	Drug status
Tgene	HLCS	P50747	DB00121	Biotin	Substrate	Small molecule	Approved\|Investigational\|Nutraceutical

Top

Related Diseases for TBCD-HLCS

Diseases associated with fusion partners.
(DisGeNet 4.0)

Partner	Gene	Disease ID	Disease name	# pubmeds	Source
Hgene	TBCD	C4310671	ENCEPHALOPATHY, PROGRESSIVE, EARLY-ONSET, WITH BRAIN ATROPHY AND THIN CORPUS CALLOSUM	4	CTD_human;GENOMICS_ENGLAND;ORPHANET;UNIPROT
Hgene	TBCD	C0025958	Microcephaly	2	CTD_human
Hgene	TBCD	C0036572	Seizures	2	CTD_human;GENOMICS_ENGLAND
Hgene	TBCD	C1956147	Microlissencephaly	2	CTD_human
Hgene	TBCD	C3853041	Severe Congenital Microcephaly	2	CTD_human
Hgene	TBCD	C0008073	Developmental Disabilities	1	CTD_human
Hgene	TBCD	C0020796	Profound Mental Retardation	1	CTD_human
Hgene	TBCD	C0022333	Jacksonian Seizure	1	CTD_human
Hgene	TBCD	C0023944	Locked-In Syndrome	1	CTD_human
Hgene	TBCD	C0025363	Mental Retardation, Psychosocial	1	CTD_human
Hgene	TBCD	C0029124	Optic Atrophy	1	CTD_human
Hgene	TBCD	C0034372	Quadriplegia	1	CTD_human
Hgene	TBCD	C0035229	Respiratory Insufficiency	1	CTD_human
Hgene	TBCD	C0037769	West Syndrome	1	GENOMICS_ENGLAND
Hgene	TBCD	C0085996	Child Development Deviations	1	CTD_human
Hgene	TBCD	C0085997	Child Development Disorders, Specific	1	CTD_human
Hgene	TBCD	C0149958	Complex partial seizures	1	CTD_human
Hgene	TBCD	C0151786	Muscle Weakness	1	CTD_human
Hgene	TBCD	C0234533	Generalized seizures	1	CTD_human
Hgene	TBCD	C0234535	Clonic Seizures	1	CTD_human
Hgene	TBCD	C0235063	Respiratory Depression	1	CTD_human
Hgene	TBCD	C0270715	Degenerative Diseases, Central Nervous System	1	CTD_human
Hgene	TBCD	C0270790	Quadriparesis	1	CTD_human
Hgene	TBCD	C0270824	Visual seizure	1	CTD_human
Hgene	TBCD	C0270844	Tonic Seizures	1	CTD_human
Hgene	TBCD	C0270846	Epileptic drop attack	1	CTD_human
Hgene	TBCD	C0422850	Seizures, Somatosensory	1	CTD_human
Hgene	TBCD	C0422852	Seizures, Auditory	1	CTD_human
Hgene	TBCD	C0422853	Olfactory seizure	1	CTD_human
Hgene	TBCD	C0422854	Gustatory seizure	1	CTD_human
Hgene	TBCD	C0422855	Vertiginous seizure	1	CTD_human
Hgene	TBCD	C0426970	Spastic Quadriplegia	1	CTD_human
Hgene	TBCD	C0431380	Cortical Dysplasia	1	CTD_human
Hgene	TBCD	C0494475	Tonic - clonic seizures	1	CTD_human
Hgene	TBCD	C0524851	Neurodegenerative Disorders	1	CTD_human
Hgene	TBCD	C0751056	Non-epileptic convulsion	1	CTD_human
Hgene	TBCD	C0751110	Single Seizure	1	CTD_human
Hgene	TBCD	C0751123	Atonic Absence Seizures	1	CTD_human
Hgene	TBCD	C0751460	Flaccid Quadriplegia	1	CTD_human
Hgene	TBCD	C0751461	Paralysis, Spinal, Quadriplegic	1	CTD_human
Hgene	TBCD	C0751494	Convulsive Seizures	1	CTD_human
Hgene	TBCD	C0751495	Seizures, Focal	1	CTD_human
Hgene	TBCD	C0751496	Seizures, Sensory	1	CTD_human
Hgene	TBCD	C0751733	Degenerative Diseases, Spinal Cord	1	CTD_human
Hgene	TBCD	C0917816	Mental deficiency	1	CTD_human
Hgene	TBCD	C1145670	Respiratory Failure	1	CTD_human
Hgene	TBCD	C1955869	Malformations of Cortical Development	1	CTD_human
Hgene	TBCD	C3495874	Nonepileptic Seizures	1	CTD_human
Hgene	TBCD	C3714756	Intellectual Disability	1	CTD_human
Hgene	TBCD	C4048158	Convulsions	1	CTD_human
Hgene	TBCD	C4316903	Absence Seizures	1	CTD_human
Hgene	TBCD	C4317109	Epileptic Seizures	1	CTD_human
Hgene	TBCD	C4317123	Myoclonic Seizures	1	CTD_human
Hgene	TBCD	C4505436	Generalized Absence Seizures	1	CTD_human
Tgene	HLCS	C0268581	Holocarboxylase Synthetase Deficiency	14	CTD_human;GENOMICS_ENGLAND;ORPHANET;UNIPROT