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Center for Computational Systems Medicine
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Fusion Gene Summary

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Fusion Gene ORF analysis

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Fusion Genomic Features

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Fusion Protein Features

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Fusion Gene Sequence

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Fusion Gene PPI analysis

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Related Drugs

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Related Diseases

Fusion gene:BCAS3-EYA2 (FusionGDB2 ID:9280)

Fusion Gene Summary for BCAS3-EYA2

check button Fusion gene summary
Fusion gene informationFusion gene name: BCAS3-EYA2
Fusion gene ID: 9280
HgeneTgene
Gene symbol

BCAS3

EYA2

Gene ID

54828

2139

Gene nameBCAS3 microtubule associated cell migration factorEYA transcriptional coactivator and phosphatase 2
SynonymsGAOB1|MAABEAB1
Cytomap

17q23.2

20q13.12

Type of geneprotein-codingprotein-coding
Descriptionbreast carcinoma-amplified sequence 3BCAS4/BCAS3 fusionRudhirabreast carcinoma amplified sequence 4/3 fusion proteinmetastasis associated antigen of breast cancerprotein Maab1eyes absent homolog 2
Modification date2020031320200313
UniProtAcc.

O00167

Ensembl transtripts involved in fusion geneENST00000589222, ENST00000407086, 
ENST00000390652, ENST00000408905, 
ENST00000588462, ENST00000585744, 
ENST00000588874, ENST00000585812, 
ENST00000357410, ENST00000327619, 
ENST00000497428, ENST00000317304, 
Fusion gene scores* DoF score53 X 35 X 17=3153515 X 15 X 10=2250
# samples 6130
** MAII scorelog2(61/31535*10)=-5.69200088030826
possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs).
DoF>8 and MAII<0		log2(30/2250*10)=-2.90689059560852
possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs).
DoF>8 and MAII<0
Context

PubMed: BCAS3 [Title/Abstract] AND EYA2 [Title/Abstract] AND fusion [Title/Abstract]

Most frequent breakpointBCAS3(59118253)-EYA2(45644820), # samples:1
Anticipated loss of major functional domain due to fusion event.
* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

check button Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez
PartnerGeneGO IDGO termPubMed ID
HgeneBCAS3

GO:0043085

positive regulation of catalytic activity

17505058

HgeneBCAS3

GO:0045944

positive regulation of transcription by RNA polymerase II

17505058

HgeneBCAS3

GO:0071391

cellular response to estrogen stimulus

17505058

TgeneEYA2

GO:0016576

histone dephosphorylation

19351884


check buttonFusion gene breakpoints across BCAS3 (5'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

check buttonFusion gene breakpoints across EYA2 (3'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

check button Fusion gene information from two resources (ChiTars 5.0 and ChimerDB 4.0)
* All genome coordinats were lifted-over on hg19.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.
SourceDiseaseSampleHgeneHchrHbpHstrandTgeneTchrTbpTstrand
ChimerDB4BRCATCGA-UL-AAZ6-01ABCAS3chr17

59118253

+EYA2chr20

45644820

+


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Fusion Gene ORF analysis for BCAS3-EYA2

check button Open reading frame (ORF) analsis of fusion genes based on Ensembl gene isoform structure.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.
ORFHenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrand
In-frameENST00000589222ENST00000357410BCAS3chr17

59118253

+EYA2chr20

45644820

+
In-frameENST00000589222ENST00000327619BCAS3chr17

59118253

+EYA2chr20

45644820

+
5CDS-intronENST00000589222ENST00000497428BCAS3chr17

59118253

+EYA2chr20

45644820

+
5CDS-intronENST00000589222ENST00000317304BCAS3chr17

59118253

+EYA2chr20

45644820

+
In-frameENST00000407086ENST00000357410BCAS3chr17

59118253

+EYA2chr20

45644820

+
In-frameENST00000407086ENST00000327619BCAS3chr17

59118253

+EYA2chr20

45644820

+
5CDS-intronENST00000407086ENST00000497428BCAS3chr17

59118253

+EYA2chr20

45644820

+
5CDS-intronENST00000407086ENST00000317304BCAS3chr17

59118253

+EYA2chr20

45644820

+
In-frameENST00000390652ENST00000357410BCAS3chr17

59118253

+EYA2chr20

45644820

+
In-frameENST00000390652ENST00000327619BCAS3chr17

59118253

+EYA2chr20

45644820

+
5CDS-intronENST00000390652ENST00000497428BCAS3chr17

59118253

+EYA2chr20

45644820

+
5CDS-intronENST00000390652ENST00000317304BCAS3chr17

59118253

+EYA2chr20

45644820

+
In-frameENST00000408905ENST00000357410BCAS3chr17

59118253

+EYA2chr20

45644820

+
In-frameENST00000408905ENST00000327619BCAS3chr17

59118253

+EYA2chr20

45644820

+
5CDS-intronENST00000408905ENST00000497428BCAS3chr17

59118253

+EYA2chr20

45644820

+
5CDS-intronENST00000408905ENST00000317304BCAS3chr17

59118253

+EYA2chr20

45644820

+
In-frameENST00000588462ENST00000357410BCAS3chr17

59118253

+EYA2chr20

45644820

+
In-frameENST00000588462ENST00000327619BCAS3chr17

59118253

+EYA2chr20

45644820

+
5CDS-intronENST00000588462ENST00000497428BCAS3chr17

59118253

+EYA2chr20

45644820

+
5CDS-intronENST00000588462ENST00000317304BCAS3chr17

59118253

+EYA2chr20

45644820

+
In-frameENST00000585744ENST00000357410BCAS3chr17

59118253

+EYA2chr20

45644820

+
In-frameENST00000585744ENST00000327619BCAS3chr17

59118253

+EYA2chr20

45644820

+
5CDS-intronENST00000585744ENST00000497428BCAS3chr17

59118253

+EYA2chr20

45644820

+
5CDS-intronENST00000585744ENST00000317304BCAS3chr17

59118253

+EYA2chr20

45644820

+
In-frameENST00000588874ENST00000357410BCAS3chr17

59118253

+EYA2chr20

45644820

+
In-frameENST00000588874ENST00000327619BCAS3chr17

59118253

+EYA2chr20

45644820

+
5CDS-intronENST00000588874ENST00000497428BCAS3chr17

59118253

+EYA2chr20

45644820

+
5CDS-intronENST00000588874ENST00000317304BCAS3chr17

59118253

+EYA2chr20

45644820

+
intron-3CDSENST00000585812ENST00000357410BCAS3chr17

59118253

+EYA2chr20

45644820

+
intron-3CDSENST00000585812ENST00000327619BCAS3chr17

59118253

+EYA2chr20

45644820

+
intron-intronENST00000585812ENST00000497428BCAS3chr17

59118253

+EYA2chr20

45644820

+
intron-intronENST00000585812ENST00000317304BCAS3chr17

59118253

+EYA2chr20

45644820

+

check buttonORFfinder result based on the fusion transcript sequence of in-frame fusion genes.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandSeq length
(transcript)		BP loci
(transcript)		Predicted start
(transcript)		Predicted stop
(transcript)		Seq length
(amino acids)
ENST00000589222BCAS3chr1759118253+ENST00000357410EYA2chr2045644820+389020976831781036
ENST00000589222BCAS3chr1759118253+ENST00000327619EYA2chr2045644820+412720976834151115
ENST00000407086BCAS3chr1759118253+ENST00000357410EYA2chr2045644820+387020774831581036
ENST00000407086BCAS3chr1759118253+ENST00000327619EYA2chr2045644820+410720774833951115
ENST00000390652BCAS3chr1759118253+ENST00000357410EYA2chr2045644820+389821053131861051
ENST00000390652BCAS3chr1759118253+ENST00000327619EYA2chr2045644820+413521053134231130
ENST00000408905BCAS3chr1759118253+ENST00000357410EYA2chr2045644820+38222029031101036
ENST00000408905BCAS3chr1759118253+ENST00000327619EYA2chr2045644820+40592029033471115
ENST00000588462BCAS3chr1759118253+ENST00000357410EYA2chr2045644820+38672074031551051
ENST00000588462BCAS3chr1759118253+ENST00000327619EYA2chr2045644820+41042074033921130
ENST00000585744BCAS3chr1759118253+ENST00000357410EYA2chr2045644820+32831490132571852
ENST00000585744BCAS3chr1759118253+ENST00000327619EYA2chr2045644820+35201490132808931
ENST00000588874BCAS3chr1759118253+ENST00000357410EYA2chr2045644820+332315301882611807
ENST00000588874BCAS3chr1759118253+ENST00000327619EYA2chr2045644820+356015301882848886

check buttonDeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandNo-coding scoreCoding score
ENST00000589222ENST00000357410BCAS3chr1759118253+EYA2chr2045644820+0.0003199290.99968004
ENST00000589222ENST00000327619BCAS3chr1759118253+EYA2chr2045644820+0.0005323680.9994677
ENST00000407086ENST00000357410BCAS3chr1759118253+EYA2chr2045644820+0.0003182020.99968183
ENST00000407086ENST00000327619BCAS3chr1759118253+EYA2chr2045644820+0.0005256230.99947435
ENST00000390652ENST00000357410BCAS3chr1759118253+EYA2chr2045644820+0.0003772950.9996227
ENST00000390652ENST00000327619BCAS3chr1759118253+EYA2chr2045644820+0.0004177720.99958223
ENST00000408905ENST00000357410BCAS3chr1759118253+EYA2chr2045644820+0.0003131340.99968684
ENST00000408905ENST00000327619BCAS3chr1759118253+EYA2chr2045644820+0.0005079050.99949205
ENST00000588462ENST00000357410BCAS3chr1759118253+EYA2chr2045644820+0.0003760230.999624
ENST00000588462ENST00000327619BCAS3chr1759118253+EYA2chr2045644820+0.0004173410.9995827
ENST00000585744ENST00000357410BCAS3chr1759118253+EYA2chr2045644820+0.0019274170.9980725
ENST00000585744ENST00000327619BCAS3chr1759118253+EYA2chr2045644820+0.0015042950.9984957
ENST00000588874ENST00000357410BCAS3chr1759118253+EYA2chr2045644820+0.0023024970.99769753
ENST00000588874ENST00000327619BCAS3chr1759118253+EYA2chr2045644820+0.0017939010.99820614

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Fusion Genomic Features for BCAS3-EYA2


check buttonFusionAI prediction of the potential fusion gene breakpoint based on the pre-mature RNA sequence context (+/- 5kb of individual partner genes, total 20kb length sequence). FusionAI is a fusion gene breakpoint classifier based on convolutional neural network by comparing the fusion positive and negative sequence context of ~ 20K fusion gene data. From here, we can have the relative potentency of the 20K genomic sequence how individual sequnce will be likely used as the gene fusion breakpoints.
HgeneHchrHbpHstrandTgeneTchrTbpTstrand1-pp (fusion gene breakpoint)
BCAS3chr1759118253+EYA2chr2045644819+1.64E-070.9999999
BCAS3chr1759118253+EYA2chr2045644819+1.64E-070.9999999

check buttonDistribution of 44 human genomic features loci across 20kb length fusion breakpoint regions. We integrated a total of 44 different types of human genomic feature loci information across five big categories including virus integration sites, repeats, structural variants, chromatin states, and gene expression regulation. More details are in help page.

check buttonDistribution of 44 human genomic features loci across 20kb length fusion breakpoint regions that are ovelapped with the top 1% feature importance score regions. More details are in help page.

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Fusion Protein Features for BCAS3-EYA2


check button Go to

FGviewer for the breakpoints of chr17:59118253-chr20:45644820

.
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
FGviewer

check buttonMain function of each fusion partner protein. (from UniProt)
HgeneTgene
.EYA2

O00167

FUNCTION: Might normally function as a transcriptional repressor. EWS-fusion-proteins (EFPS) may play a role in the tumorigenic process. They may disturb gene expression by mimicking, or interfering with the normal function of CTD-POLII within the transcription initiation complex. They may also contribute to an aberrant activation of the fusion protein target genes.FUNCTION: Functions both as protein phosphatase and as transcriptional coactivator for SIX1, and probably also for SIX2, SIX4 and SIX5 (PubMed:12500905, PubMed:23435380). Tyrosine phosphatase that dephosphorylates 'Tyr-142' of histone H2AX (H2AXY142ph) and promotes efficient DNA repair via the recruitment of DNA repair complexes containing MDC1. 'Tyr-142' phosphorylation of histone H2AX plays a central role in DNA repair and acts as a mark that distinguishes between apoptotic and repair responses to genotoxic stress (PubMed:19351884). Its function as histone phosphatase may contribute to its function in transcription regulation during organogenesis. Plays an important role in hypaxial muscle development together with SIX1 and DACH2; in this it is functionally redundant with EYA1 (PubMed:12500905). {ECO:0000269|PubMed:12500905, ECO:0000269|PubMed:19351884, ECO:0000269|PubMed:21706047, ECO:0000269|PubMed:23435380}.

check buttonRetention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at

download page


* Minus value of BPloci means that the break pointn is located before the CDS.
- In-frame and retained protein feature among the 13 regional features.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note

- In-frame and not-retained protein feature among the 13 regional features.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note


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Fusion Gene Sequence for BCAS3-EYA2


check button For in-frame fusion transcripts, we provide the fusion transcript sequences and fusion amino acid sequences. To have fusion amino acid sequence, we ran ORFfinder and chose the longest ORF among the all predicted ones.
>In-frame_ENST00000589222_ENST00000357410_TCGA-UL-AAZ6-01A_BCAS3_chr17_59118253_+_EYA2_chr20_45644820_length(transcript)=3890nt_BP=2097nt
CTCGGGGAGACTCCAAACAGTGAGCCTAGAGCTGGAGACTAGCGTTAACCGGCGGGGCGGCCGGTTTTATGAATGAAGCTATGGCTACAG
ATTCCCCAAGAAGACCCAGTCGTTGTACTGGTGGAGTTGTGGTTCGCCCCCAGGCTGTCACAGAGCAGTCCTACATGGAAAGTGTTGTGA
CTTTTCTGCAGGATGTTGTGCCACAGGCTTACAGTGGAACACCTCTAACAGAAGAAAAGGAGAAAATAGTCTGGGTCAGATTTGAAAATG
CAGATTTAAATGATACATCAAGAAATCTGGAATTTCATGAAATACATAGTACTGGGAATGAACCGCCTTTGTTGATTATGATTGGCTACA
GTGATGGAATGCAGGTCTGGAGCATCCCTATCAGTGGTGAAGCACAAGAGCTCTTCTCTGTTCGACATGGCCCAATTCGAGCGGCTAGAA
TCTTGCCTGCTCCACAGTTTGGTGCTCAAAAATGTGATAACTTTGCTGAAAAAAGACCCCTCCTTGGTGTTTGTAAGAGCATTGGATCTT
CTGGCACAAGCCCACCGTACTGTTGTGTGGATCTGTATTCACTTCGTACTGGGGAGATGGTCAAGTCCATTCAATTTAAGACACCTATTT
ATGATCTCCATTGCAATAAACGGATCCTTGTCGTAGTCTTGCAGGAGAAAATTGCTGCCTTTGATAGCTGTACTTTCACGAAGAAATTCT
TTGTTACAAGCTGCTATCCATGTCCAGGGCCAAACATGAATCCTATTGCTCTTGGGAGCCGCTGGCTTGCTTATGCAGAAAACAAGTTGA
TTCGATGTCATCAGTCCCGTGGTGGAGCCTGTGGAGACAACATTCAGTCTTATACTGCCACAGTCATTAGTGCTGCTAAAACATTGAAAA
GTGGCCTGACAATGGTAGGGAAAGTGGTGACTCAGCTGACAGGCACACTGCCTTCAGGTGTGACAGAAGATGATGTTGCCATCCACAGTA
ATTCACGGCGGAGTCCTTTGGTCCCAGGCATCATCACAGTTATTGACACCGAAACCGTTGGAGAGGGCCAGGTGCTTGTGAGTGAGGATT
CTGACAGTGATGGCATTGTGGCCCACTTCCCTGCCCATGAGAAGCCAGTGTGCTGCATGGCTTTTAATACAAGTGGAATGCTTCTAGTCA
CAACAGACACCCTTGGCCATGACTTTCATGTCTTCCAAATTCTGACTCATCCTTGGTCCTCATCACAATGTGCTGTCCACCATCTGTATA
CTCTTCACAGGGGAGAAACTGAAGCCAAAGTACAGGACATCTGCTTCAGCCATGACTGTCGCTGGGTTGTGGTCAGTACTCTCCGGGGTA
CTTCCCACGTTTTCCCCATCAACCCTTATGGTGGCCAGCCTTGTGTTCGTACACATATGTCACCACGAGTAGTGAATCGCATGAGCCGTT
TCCAGAAAAGTGCTGGACTGGAAGAGATTGAACAAGAACTGACGTCTAAGCAAGGAGGTCGCTGTAGCCCTGTTCCAGGTCTATCAAGCA
GCCCTTCTGGGTCACCCTTGCATGGGAAACTGAACAGCCAAGACTCCTATAACAATTTTACCAACAACAACCCTGGCAACCCTCGGCTCT
CTCCTCTTCCCAGCTTGATGGTAGTGATGCCTCTTGCACAAATCAAGCAGCCAATGACATTGGGGACCATCACCAAACGAACCGGCAAAG
TTAAACCTCCTCCACAAATTTCACCCAGCAAATCGATGGGCGGAGAATTTTGTGTGGCTGCTATCTTCGGAACATCCAGGTCATGGTTTG
CAAATAATGCAGGTCTGAAAAGAGAAAAAGATCAGTCCAAACAAGTTGTAGTTGAGTCCCTGTACATTATCAGTTGCTATGGCACCTTAG
TGGAACACATGATGGAGCCGCGACCCCTCAGCACTGCACCCAAGATTAGTGACGACACACCACTGGAAATGATGACATCGCCTCGAGCCA
GCTGGACTCTGGTTAGAACCCCTCAATGGAATGAATTGCAGCCACCGTTTAATGCAAACCACCCTCTGCTCCTCGCTGCAGATGCAGTAC
AGTATTATCAGTTCCTGCTTGCTGGCCGCATCAAGACAGAAGACAGCTTGAACCATTCCCCTGGCCAGAGTGGATTCCTCAGCTATGGCT
CCAGCTTCAGCACCTCACCCACTGGACAGAGCCCATACACCTACCAGATGCACGGCACAACAGGGTTCTATCAAGGAGGAAATGGACTGG
GCAACGCAGCCGGTTTCGGGAGTGTGCACCAGGACTATCCTTCCTACCCCGGCTTCCCCCAGAGCCAGTACCCCCAGTATTACGGCTCAT
CCTACAACCCTCCCTACGTCCCGGCCAGCAGCATCTGCCCTTCGCCCCTCTCCACGTCCACCTACGTCCTCCAGGAGGCATCTCACAACG
TCCCCAACCAGAGTTCCGAGTCACTTGCTGGTGAATACAACACACACAATGGACCTTCCACACCAGCGAAAGAGGGAGACACAGACAGGC
CGCACCGGGCCTCCGACGGGAAGCTCCGAGGCCGGTCTAAGAGGAGCAGTGACCCGTCCCCGGCAGGGGACAATGAGATTGAGCGTGTGT
TCGTGTGGGACTTGGATGAGACAATAATTATTTTTCACTCCTTACTCACGGGGACATTTGCATCCAGATACGGGAAGGACACCACGACGT
CCGTGCGCATTGGCCTTATGATGGAAGAGATGATCTTCAACCTTGCAGATACACATCTGTTCTTCAATGACCTGGAGGATTGTGACCAGA
TCCACGTTGATGACGTCTCATCAGATGACAATGGCCAAGATTTAAGCACATACAACTTCTCCGCTGACGGCTTCCACAGTTCGGCCCCAG
GAGCCAACCTGTGCCTGGGCTCTGGCGTGCACGGCGGCGTGGACTGGATGAGGAAGCTGGCCTTCCGCTACCGGCGGGTGAAGGAGATGT
ACAATACCTACAAGAACAACGTTGGTGGGAAGGAGAGCTGCTTCGAGAGGATAATGCAGAGATTCGGCAGAAAAGCTGTCTACGTGGTGA
TCGGTGATGGTGTGGAAGAGGAGCAAGGAGCGAAAAAGCACAACATGCCTTTCTGGCGGATATCCTGCCACGCAGACCTGGAGGCACTGA
GGCACGCCCTGGAGCTGGAGTATTTATAGCAGGATCAGCAGCATCTCCACCTGCCATCTCACCCTCAGACCCCCTCGCCTTCCCCACCTC
CCCACCGAGAACTCCAGAGACCCAGATGTTGGACACCAGGAAGGGGCCCCACAGCCGAGACGACGTGTCCAGTGACCATCTCAGAAGCCG
TCCATCAGTCCAAATGGGGGTTCTGAGAAGGAAAGTACCCAACATTGGCTTCGGAGTATTTGACTTTGGGGAAAAGGGCTGGCTCGGAGT
CTAGACTCTTCTGTAAGACTCACAGAACAAAAGCAAGGAATTGCTGATTTGGGGGGTGCCTGGTGATGAGGAGGGGATGGGTTTGTCTTG
TCTTCTTTTTAATTTATGGACTAGTCTCATTACTCCGGAATTATGCTCTTGTACCTGTGTGGCTGGGTTTCTTAGTCGTTGGTTTGGTTT
GGTTTTTTGAACTGGTATGTGGGGTGGTTCACAGTTCTAATGTAAGCACTCTATTCTCCAAGTTGTGCTTTGTGGGGACAATCATTCTTT
GAACATTAGAGAGGAAGGCAGTTCAAGCTGTTGAAAAGACTATTGCTTATTTTTGTTTTTAAAGACCTACTTGACGTCATGTGGACAGTG
CACGTGCCTTACGCTACATCTTGTTTTCTAGGAAGAGGGGGATGCTGGGAAGGAATGGGTGCTTTGTGATGGATAAAAGGCATTAAATAA

>In-frame_ENST00000589222_ENST00000357410_TCGA-UL-AAZ6-01A_BCAS3_chr17_59118253_+_EYA2_chr20_45644820_length(amino acids)=1036AA_start in transcript=68_stop in transcript=3178
MNEAMATDSPRRPSRCTGGVVVRPQAVTEQSYMESVVTFLQDVVPQAYSGTPLTEEKEKIVWVRFENADLNDTSRNLEFHEIHSTGNEPP
LLIMIGYSDGMQVWSIPISGEAQELFSVRHGPIRAARILPAPQFGAQKCDNFAEKRPLLGVCKSIGSSGTSPPYCCVDLYSLRTGEMVKS
IQFKTPIYDLHCNKRILVVVLQEKIAAFDSCTFTKKFFVTSCYPCPGPNMNPIALGSRWLAYAENKLIRCHQSRGGACGDNIQSYTATVI
SAAKTLKSGLTMVGKVVTQLTGTLPSGVTEDDVAIHSNSRRSPLVPGIITVIDTETVGEGQVLVSEDSDSDGIVAHFPAHEKPVCCMAFN
TSGMLLVTTDTLGHDFHVFQILTHPWSSSQCAVHHLYTLHRGETEAKVQDICFSHDCRWVVVSTLRGTSHVFPINPYGGQPCVRTHMSPR
VVNRMSRFQKSAGLEEIEQELTSKQGGRCSPVPGLSSSPSGSPLHGKLNSQDSYNNFTNNNPGNPRLSPLPSLMVVMPLAQIKQPMTLGT
ITKRTGKVKPPPQISPSKSMGGEFCVAAIFGTSRSWFANNAGLKREKDQSKQVVVESLYIISCYGTLVEHMMEPRPLSTAPKISDDTPLE
MMTSPRASWTLVRTPQWNELQPPFNANHPLLLAADAVQYYQFLLAGRIKTEDSLNHSPGQSGFLSYGSSFSTSPTGQSPYTYQMHGTTGF
YQGGNGLGNAAGFGSVHQDYPSYPGFPQSQYPQYYGSSYNPPYVPASSICPSPLSTSTYVLQEASHNVPNQSSESLAGEYNTHNGPSTPA
KEGDTDRPHRASDGKLRGRSKRSSDPSPAGDNEIERVFVWDLDETIIIFHSLLTGTFASRYGKDTTTSVRIGLMMEEMIFNLADTHLFFN
DLEDCDQIHVDDVSSDDNGQDLSTYNFSADGFHSSAPGANLCLGSGVHGGVDWMRKLAFRYRRVKEMYNTYKNNVGGKESCFERIMQRFG

--------------------------------------------------------------
>In-frame_ENST00000589222_ENST00000327619_TCGA-UL-AAZ6-01A_BCAS3_chr17_59118253_+_EYA2_chr20_45644820_length(transcript)=4127nt_BP=2097nt
CTCGGGGAGACTCCAAACAGTGAGCCTAGAGCTGGAGACTAGCGTTAACCGGCGGGGCGGCCGGTTTTATGAATGAAGCTATGGCTACAG
ATTCCCCAAGAAGACCCAGTCGTTGTACTGGTGGAGTTGTGGTTCGCCCCCAGGCTGTCACAGAGCAGTCCTACATGGAAAGTGTTGTGA
CTTTTCTGCAGGATGTTGTGCCACAGGCTTACAGTGGAACACCTCTAACAGAAGAAAAGGAGAAAATAGTCTGGGTCAGATTTGAAAATG
CAGATTTAAATGATACATCAAGAAATCTGGAATTTCATGAAATACATAGTACTGGGAATGAACCGCCTTTGTTGATTATGATTGGCTACA
GTGATGGAATGCAGGTCTGGAGCATCCCTATCAGTGGTGAAGCACAAGAGCTCTTCTCTGTTCGACATGGCCCAATTCGAGCGGCTAGAA
TCTTGCCTGCTCCACAGTTTGGTGCTCAAAAATGTGATAACTTTGCTGAAAAAAGACCCCTCCTTGGTGTTTGTAAGAGCATTGGATCTT
CTGGCACAAGCCCACCGTACTGTTGTGTGGATCTGTATTCACTTCGTACTGGGGAGATGGTCAAGTCCATTCAATTTAAGACACCTATTT
ATGATCTCCATTGCAATAAACGGATCCTTGTCGTAGTCTTGCAGGAGAAAATTGCTGCCTTTGATAGCTGTACTTTCACGAAGAAATTCT
TTGTTACAAGCTGCTATCCATGTCCAGGGCCAAACATGAATCCTATTGCTCTTGGGAGCCGCTGGCTTGCTTATGCAGAAAACAAGTTGA
TTCGATGTCATCAGTCCCGTGGTGGAGCCTGTGGAGACAACATTCAGTCTTATACTGCCACAGTCATTAGTGCTGCTAAAACATTGAAAA
GTGGCCTGACAATGGTAGGGAAAGTGGTGACTCAGCTGACAGGCACACTGCCTTCAGGTGTGACAGAAGATGATGTTGCCATCCACAGTA
ATTCACGGCGGAGTCCTTTGGTCCCAGGCATCATCACAGTTATTGACACCGAAACCGTTGGAGAGGGCCAGGTGCTTGTGAGTGAGGATT
CTGACAGTGATGGCATTGTGGCCCACTTCCCTGCCCATGAGAAGCCAGTGTGCTGCATGGCTTTTAATACAAGTGGAATGCTTCTAGTCA
CAACAGACACCCTTGGCCATGACTTTCATGTCTTCCAAATTCTGACTCATCCTTGGTCCTCATCACAATGTGCTGTCCACCATCTGTATA
CTCTTCACAGGGGAGAAACTGAAGCCAAAGTACAGGACATCTGCTTCAGCCATGACTGTCGCTGGGTTGTGGTCAGTACTCTCCGGGGTA
CTTCCCACGTTTTCCCCATCAACCCTTATGGTGGCCAGCCTTGTGTTCGTACACATATGTCACCACGAGTAGTGAATCGCATGAGCCGTT
TCCAGAAAAGTGCTGGACTGGAAGAGATTGAACAAGAACTGACGTCTAAGCAAGGAGGTCGCTGTAGCCCTGTTCCAGGTCTATCAAGCA
GCCCTTCTGGGTCACCCTTGCATGGGAAACTGAACAGCCAAGACTCCTATAACAATTTTACCAACAACAACCCTGGCAACCCTCGGCTCT
CTCCTCTTCCCAGCTTGATGGTAGTGATGCCTCTTGCACAAATCAAGCAGCCAATGACATTGGGGACCATCACCAAACGAACCGGCAAAG
TTAAACCTCCTCCACAAATTTCACCCAGCAAATCGATGGGCGGAGAATTTTGTGTGGCTGCTATCTTCGGAACATCCAGGTCATGGTTTG
CAAATAATGCAGGTCTGAAAAGAGAAAAAGATCAGTCCAAACAAGTTGTAGTTGAGTCCCTGTACATTATCAGTTGCTATGGCACCTTAG
TGGAACACATGATGGAGCCGCGACCCCTCAGCACTGCACCCAAGATTAGTGACGACACACCACTGGAAATGATGACATCGCCTCGAGCCA
GCTGGACTCTGGTTAGAACCCCTCAATGGAATGAATTGCAGCCACCGTTTAATGCAAACCACCCTCTGCTCCTCGCTGCAGATGCAGTAC
AGTATTATCAGTTCCTGCTTGCTGGCCGCATCAAGACAGAAGACAGCTTGAACCATTCCCCTGGCCAGAGTGGATTCCTCAGCTATGGCT
CCAGCTTCAGCACCTCACCCACTGGACAGAGCCCATACACCTACCAGATGCACGGCACAACAGGGTTCTATCAAGGAGGAAATGGACTGG
GCAACGCAGCCGGTTTCGGGAGTGTGCACCAGGACTATCCTTCCTACCCCGGCTTCCCCCAGAGCCAGTACCCCCAGTATTACGGCTCAT
CCTACAACCCTCCCTACGTCCCGGCCAGCAGCATCTGCCCTTCGCCCCTCTCCACGTCCACCTACGTCCTCCAGGAGGCATCTCACAACG
TCCCCAACCAGAGTTCCGAGTCACTTGCTGGTGAATACAACACACACAATGGACCTTCCACACCAGCGAAAGAGGGAGACACAGACAGGC
CGCACCGGGCCTCCGACGGGAAGCTCCGAGGCCGGTCTAAGAGGAGCAGTGACCCGTCCCCGGCAGGGGACAATGAGATTGAGCGTGTGT
TCGTGTGGGACTTGGATGAGACAATAATTATTTTTCACTCCTTACTCACGGGGACATTTGCATCCAGATACGGGAAGGACACCACGACGT
CCGTGCGCATTGGCCTTATGATGGAAGAGATGATCTTCAACCTTGCAGATACACATCTGTTCTTCAATGACCTGGAGGATTGTGACCAGA
TCCACGTTGATGACGTCTCATCAGATGACAATGGCCAAGATTTAAGCACATACAACTTCTCCGCTGACGGCTTCCACAGTTCGGCCCCAG
GAGCCAACCTGTGCCTGGGCTCTGGCGTGCACGGCGGCGTGGACTGGATGAGGAAGCTGGCCTTCCGCTACCGGCGGGTGAAGGAGATGT
ACAATACCTACAAGAACAACGTTGGTGGGTTGATAGGCACTCCCAAAAGGGAGACCTGGCTACAGCTCCGAGCTGAGCTGGAAGCTCTCA
CAGACCTCTGGCTGACCCACTCCCTGAAGGCACTAAACCTCATCAACTCCCGGCCCAACTGTGTCAATGTGCTGGTCACCACCACTCAAC
TAATTCCTGCCCTGGCCAAAGTCCTGCTATATGGCCTGGGGTCTGTGTTTCCTATTGAGAACATCTACAGTGCAACCAAGACAGGGAAGG
AGAGCTGCTTCGAGAGGATAATGCAGAGATTCGGCAGAAAAGCTGTCTACGTGGTGATCGGTGATGGTGTGGAAGAGGAGCAAGGAGCGA
AAAAGCACAACATGCCTTTCTGGCGGATATCCTGCCACGCAGACCTGGAGGCACTGAGGCACGCCCTGGAGCTGGAGTATTTATAGCAGG
ATCAGCAGCATCTCCACCTGCCATCTCACCCTCAGACCCCCTCGCCTTCCCCACCTCCCCACCGAGAACTCCAGAGACCCAGATGTTGGA
CACCAGGAAGGGGCCCCACAGCCGAGACGACGTGTCCAGTGACCATCTCAGAAGCCGTCCATCAGTCCAAATGGGGGTTCTGAGAAGGAA
AGTACCCAACATTGGCTTCGGAGTATTTGACTTTGGGGAAAAGGGCTGGCTCGGAGTCTAGACTCTTCTGTAAGACTCACAGAACAAAAG
CAAGGAATTGCTGATTTGGGGGGTGCCTGGTGATGAGGAGGGGATGGGTTTGTCTTGTCTTCTTTTTAATTTATGGACTAGTCTCATTAC
TCCGGAATTATGCTCTTGTACCTGTGTGGCTGGGTTTCTTAGTCGTTGGTTTGGTTTGGTTTTTTGAACTGGTATGTGGGGTGGTTCACA
GTTCTAATGTAAGCACTCTATTCTCCAAGTTGTGCTTTGTGGGGACAATCATTCTTTGAACATTAGAGAGGAAGGCAGTTCAAGCTGTTG
AAAAGACTATTGCTTATTTTTGTTTTTAAAGACCTACTTGACGTCATGTGGACAGTGCACGTGCCTTACGCTACATCTTGTTTTCTAGGA

>In-frame_ENST00000589222_ENST00000327619_TCGA-UL-AAZ6-01A_BCAS3_chr17_59118253_+_EYA2_chr20_45644820_length(amino acids)=1115AA_start in transcript=68_stop in transcript=3415
MNEAMATDSPRRPSRCTGGVVVRPQAVTEQSYMESVVTFLQDVVPQAYSGTPLTEEKEKIVWVRFENADLNDTSRNLEFHEIHSTGNEPP
LLIMIGYSDGMQVWSIPISGEAQELFSVRHGPIRAARILPAPQFGAQKCDNFAEKRPLLGVCKSIGSSGTSPPYCCVDLYSLRTGEMVKS
IQFKTPIYDLHCNKRILVVVLQEKIAAFDSCTFTKKFFVTSCYPCPGPNMNPIALGSRWLAYAENKLIRCHQSRGGACGDNIQSYTATVI
SAAKTLKSGLTMVGKVVTQLTGTLPSGVTEDDVAIHSNSRRSPLVPGIITVIDTETVGEGQVLVSEDSDSDGIVAHFPAHEKPVCCMAFN
TSGMLLVTTDTLGHDFHVFQILTHPWSSSQCAVHHLYTLHRGETEAKVQDICFSHDCRWVVVSTLRGTSHVFPINPYGGQPCVRTHMSPR
VVNRMSRFQKSAGLEEIEQELTSKQGGRCSPVPGLSSSPSGSPLHGKLNSQDSYNNFTNNNPGNPRLSPLPSLMVVMPLAQIKQPMTLGT
ITKRTGKVKPPPQISPSKSMGGEFCVAAIFGTSRSWFANNAGLKREKDQSKQVVVESLYIISCYGTLVEHMMEPRPLSTAPKISDDTPLE
MMTSPRASWTLVRTPQWNELQPPFNANHPLLLAADAVQYYQFLLAGRIKTEDSLNHSPGQSGFLSYGSSFSTSPTGQSPYTYQMHGTTGF
YQGGNGLGNAAGFGSVHQDYPSYPGFPQSQYPQYYGSSYNPPYVPASSICPSPLSTSTYVLQEASHNVPNQSSESLAGEYNTHNGPSTPA
KEGDTDRPHRASDGKLRGRSKRSSDPSPAGDNEIERVFVWDLDETIIIFHSLLTGTFASRYGKDTTTSVRIGLMMEEMIFNLADTHLFFN
DLEDCDQIHVDDVSSDDNGQDLSTYNFSADGFHSSAPGANLCLGSGVHGGVDWMRKLAFRYRRVKEMYNTYKNNVGGLIGTPKRETWLQL
RAELEALTDLWLTHSLKALNLINSRPNCVNVLVTTTQLIPALAKVLLYGLGSVFPIENIYSATKTGKESCFERIMQRFGRKAVYVVIGDG

--------------------------------------------------------------
>In-frame_ENST00000407086_ENST00000357410_TCGA-UL-AAZ6-01A_BCAS3_chr17_59118253_+_EYA2_chr20_45644820_length(transcript)=3870nt_BP=2077nt
TGAGCCTAGAGCTGGAGACTAGCGTTAACCGGCGGGGCGGCCGGTTTTATGAATGAAGCTATGGCTACAGATTCCCCAAGAAGACCCAGT
CGTTGTACTGGTGGAGTTGTGGTTCGCCCCCAGGCTGTCACAGAGCAGTCCTACATGGAAAGTGTTGTGACTTTTCTGCAGGATGTTGTG
CCACAGGCTTACAGTGGAACACCTCTAACAGAAGAAAAGGAGAAAATAGTCTGGGTCAGATTTGAAAATGCAGATTTAAATGATACATCA
AGAAATCTGGAATTTCATGAAATACATAGTACTGGGAATGAACCGCCTTTGTTGATTATGATTGGCTACAGTGATGGAATGCAGGTCTGG
AGCATCCCTATCAGTGGTGAAGCACAAGAGCTCTTCTCTGTTCGACATGGCCCAATTCGAGCGGCTAGAATCTTGCCTGCTCCACAGTTT
GGTGCTCAAAAATGTGATAACTTTGCTGAAAAAAGACCCCTCCTTGGTGTTTGTAAGAGCATTGGATCTTCTGGCACAAGCCCACCGTAC
TGTTGTGTGGATCTGTATTCACTTCGTACTGGGGAGATGGTCAAGTCCATTCAATTTAAGACACCTATTTATGATCTCCATTGCAATAAA
CGGATCCTTGTCGTAGTCTTGCAGGAGAAAATTGCTGCCTTTGATAGCTGTACTTTCACGAAGAAATTCTTTGTTACAAGCTGCTATCCA
TGTCCAGGGCCAAACATGAATCCTATTGCTCTTGGGAGCCGCTGGCTTGCTTATGCAGAAAACAAGTTGATTCGATGTCATCAGTCCCGT
GGTGGAGCCTGTGGAGACAACATTCAGTCTTATACTGCCACAGTCATTAGTGCTGCTAAAACATTGAAAAGTGGCCTGACAATGGTAGGG
AAAGTGGTGACTCAGCTGACAGGCACACTGCCTTCAGGTGTGACAGAAGATGATGTTGCCATCCACAGTAATTCACGGCGGAGTCCTTTG
GTCCCAGGCATCATCACAGTTATTGACACCGAAACCGTTGGAGAGGGCCAGGTGCTTGTGAGTGAGGATTCTGACAGTGATGGCATTGTG
GCCCACTTCCCTGCCCATGAGAAGCCAGTGTGCTGCATGGCTTTTAATACAAGTGGAATGCTTCTAGTCACAACAGACACCCTTGGCCAT
GACTTTCATGTCTTCCAAATTCTGACTCATCCTTGGTCCTCATCACAATGTGCTGTCCACCATCTGTATACTCTTCACAGGGGAGAAACT
GAAGCCAAAGTACAGGACATCTGCTTCAGCCATGACTGTCGCTGGGTTGTGGTCAGTACTCTCCGGGGTACTTCCCACGTTTTCCCCATC
AACCCTTATGGTGGCCAGCCTTGTGTTCGTACACATATGTCACCACGAGTAGTGAATCGCATGAGCCGTTTCCAGAAAAGTGCTGGACTG
GAAGAGATTGAACAAGAACTGACGTCTAAGCAAGGAGGTCGCTGTAGCCCTGTTCCAGGTCTATCAAGCAGCCCTTCTGGGTCACCCTTG
CATGGGAAACTGAACAGCCAAGACTCCTATAACAATTTTACCAACAACAACCCTGGCAACCCTCGGCTCTCTCCTCTTCCCAGCTTGATG
GTAGTGATGCCTCTTGCACAAATCAAGCAGCCAATGACATTGGGGACCATCACCAAACGAACCGGCAAAGTTAAACCTCCTCCACAAATT
TCACCCAGCAAATCGATGGGCGGAGAATTTTGTGTGGCTGCTATCTTCGGAACATCCAGGTCATGGTTTGCAAATAATGCAGGTCTGAAA
AGAGAAAAAGATCAGTCCAAACAAGTTGTAGTTGAGTCCCTGTACATTATCAGTTGCTATGGCACCTTAGTGGAACACATGATGGAGCCG
CGACCCCTCAGCACTGCACCCAAGATTAGTGACGACACACCACTGGAAATGATGACATCGCCTCGAGCCAGCTGGACTCTGGTTAGAACC
CCTCAATGGAATGAATTGCAGCCACCGTTTAATGCAAACCACCCTCTGCTCCTCGCTGCAGATGCAGTACAGTATTATCAGTTCCTGCTT
GCTGGCCGCATCAAGACAGAAGACAGCTTGAACCATTCCCCTGGCCAGAGTGGATTCCTCAGCTATGGCTCCAGCTTCAGCACCTCACCC
ACTGGACAGAGCCCATACACCTACCAGATGCACGGCACAACAGGGTTCTATCAAGGAGGAAATGGACTGGGCAACGCAGCCGGTTTCGGG
AGTGTGCACCAGGACTATCCTTCCTACCCCGGCTTCCCCCAGAGCCAGTACCCCCAGTATTACGGCTCATCCTACAACCCTCCCTACGTC
CCGGCCAGCAGCATCTGCCCTTCGCCCCTCTCCACGTCCACCTACGTCCTCCAGGAGGCATCTCACAACGTCCCCAACCAGAGTTCCGAG
TCACTTGCTGGTGAATACAACACACACAATGGACCTTCCACACCAGCGAAAGAGGGAGACACAGACAGGCCGCACCGGGCCTCCGACGGG
AAGCTCCGAGGCCGGTCTAAGAGGAGCAGTGACCCGTCCCCGGCAGGGGACAATGAGATTGAGCGTGTGTTCGTGTGGGACTTGGATGAG
ACAATAATTATTTTTCACTCCTTACTCACGGGGACATTTGCATCCAGATACGGGAAGGACACCACGACGTCCGTGCGCATTGGCCTTATG
ATGGAAGAGATGATCTTCAACCTTGCAGATACACATCTGTTCTTCAATGACCTGGAGGATTGTGACCAGATCCACGTTGATGACGTCTCA
TCAGATGACAATGGCCAAGATTTAAGCACATACAACTTCTCCGCTGACGGCTTCCACAGTTCGGCCCCAGGAGCCAACCTGTGCCTGGGC
TCTGGCGTGCACGGCGGCGTGGACTGGATGAGGAAGCTGGCCTTCCGCTACCGGCGGGTGAAGGAGATGTACAATACCTACAAGAACAAC
GTTGGTGGGAAGGAGAGCTGCTTCGAGAGGATAATGCAGAGATTCGGCAGAAAAGCTGTCTACGTGGTGATCGGTGATGGTGTGGAAGAG
GAGCAAGGAGCGAAAAAGCACAACATGCCTTTCTGGCGGATATCCTGCCACGCAGACCTGGAGGCACTGAGGCACGCCCTGGAGCTGGAG
TATTTATAGCAGGATCAGCAGCATCTCCACCTGCCATCTCACCCTCAGACCCCCTCGCCTTCCCCACCTCCCCACCGAGAACTCCAGAGA
CCCAGATGTTGGACACCAGGAAGGGGCCCCACAGCCGAGACGACGTGTCCAGTGACCATCTCAGAAGCCGTCCATCAGTCCAAATGGGGG
TTCTGAGAAGGAAAGTACCCAACATTGGCTTCGGAGTATTTGACTTTGGGGAAAAGGGCTGGCTCGGAGTCTAGACTCTTCTGTAAGACT
CACAGAACAAAAGCAAGGAATTGCTGATTTGGGGGGTGCCTGGTGATGAGGAGGGGATGGGTTTGTCTTGTCTTCTTTTTAATTTATGGA
CTAGTCTCATTACTCCGGAATTATGCTCTTGTACCTGTGTGGCTGGGTTTCTTAGTCGTTGGTTTGGTTTGGTTTTTTGAACTGGTATGT
GGGGTGGTTCACAGTTCTAATGTAAGCACTCTATTCTCCAAGTTGTGCTTTGTGGGGACAATCATTCTTTGAACATTAGAGAGGAAGGCA
GTTCAAGCTGTTGAAAAGACTATTGCTTATTTTTGTTTTTAAAGACCTACTTGACGTCATGTGGACAGTGCACGTGCCTTACGCTACATC
TTGTTTTCTAGGAAGAGGGGGATGCTGGGAAGGAATGGGTGCTTTGTGATGGATAAAAGGCATTAAATAAAACCACGTTTACATTTTGAA

>In-frame_ENST00000407086_ENST00000357410_TCGA-UL-AAZ6-01A_BCAS3_chr17_59118253_+_EYA2_chr20_45644820_length(amino acids)=1036AA_start in transcript=48_stop in transcript=3158
MNEAMATDSPRRPSRCTGGVVVRPQAVTEQSYMESVVTFLQDVVPQAYSGTPLTEEKEKIVWVRFENADLNDTSRNLEFHEIHSTGNEPP
LLIMIGYSDGMQVWSIPISGEAQELFSVRHGPIRAARILPAPQFGAQKCDNFAEKRPLLGVCKSIGSSGTSPPYCCVDLYSLRTGEMVKS
IQFKTPIYDLHCNKRILVVVLQEKIAAFDSCTFTKKFFVTSCYPCPGPNMNPIALGSRWLAYAENKLIRCHQSRGGACGDNIQSYTATVI
SAAKTLKSGLTMVGKVVTQLTGTLPSGVTEDDVAIHSNSRRSPLVPGIITVIDTETVGEGQVLVSEDSDSDGIVAHFPAHEKPVCCMAFN
TSGMLLVTTDTLGHDFHVFQILTHPWSSSQCAVHHLYTLHRGETEAKVQDICFSHDCRWVVVSTLRGTSHVFPINPYGGQPCVRTHMSPR
VVNRMSRFQKSAGLEEIEQELTSKQGGRCSPVPGLSSSPSGSPLHGKLNSQDSYNNFTNNNPGNPRLSPLPSLMVVMPLAQIKQPMTLGT
ITKRTGKVKPPPQISPSKSMGGEFCVAAIFGTSRSWFANNAGLKREKDQSKQVVVESLYIISCYGTLVEHMMEPRPLSTAPKISDDTPLE
MMTSPRASWTLVRTPQWNELQPPFNANHPLLLAADAVQYYQFLLAGRIKTEDSLNHSPGQSGFLSYGSSFSTSPTGQSPYTYQMHGTTGF
YQGGNGLGNAAGFGSVHQDYPSYPGFPQSQYPQYYGSSYNPPYVPASSICPSPLSTSTYVLQEASHNVPNQSSESLAGEYNTHNGPSTPA
KEGDTDRPHRASDGKLRGRSKRSSDPSPAGDNEIERVFVWDLDETIIIFHSLLTGTFASRYGKDTTTSVRIGLMMEEMIFNLADTHLFFN
DLEDCDQIHVDDVSSDDNGQDLSTYNFSADGFHSSAPGANLCLGSGVHGGVDWMRKLAFRYRRVKEMYNTYKNNVGGKESCFERIMQRFG

--------------------------------------------------------------
>In-frame_ENST00000407086_ENST00000327619_TCGA-UL-AAZ6-01A_BCAS3_chr17_59118253_+_EYA2_chr20_45644820_length(transcript)=4107nt_BP=2077nt
TGAGCCTAGAGCTGGAGACTAGCGTTAACCGGCGGGGCGGCCGGTTTTATGAATGAAGCTATGGCTACAGATTCCCCAAGAAGACCCAGT
CGTTGTACTGGTGGAGTTGTGGTTCGCCCCCAGGCTGTCACAGAGCAGTCCTACATGGAAAGTGTTGTGACTTTTCTGCAGGATGTTGTG
CCACAGGCTTACAGTGGAACACCTCTAACAGAAGAAAAGGAGAAAATAGTCTGGGTCAGATTTGAAAATGCAGATTTAAATGATACATCA
AGAAATCTGGAATTTCATGAAATACATAGTACTGGGAATGAACCGCCTTTGTTGATTATGATTGGCTACAGTGATGGAATGCAGGTCTGG
AGCATCCCTATCAGTGGTGAAGCACAAGAGCTCTTCTCTGTTCGACATGGCCCAATTCGAGCGGCTAGAATCTTGCCTGCTCCACAGTTT
GGTGCTCAAAAATGTGATAACTTTGCTGAAAAAAGACCCCTCCTTGGTGTTTGTAAGAGCATTGGATCTTCTGGCACAAGCCCACCGTAC
TGTTGTGTGGATCTGTATTCACTTCGTACTGGGGAGATGGTCAAGTCCATTCAATTTAAGACACCTATTTATGATCTCCATTGCAATAAA
CGGATCCTTGTCGTAGTCTTGCAGGAGAAAATTGCTGCCTTTGATAGCTGTACTTTCACGAAGAAATTCTTTGTTACAAGCTGCTATCCA
TGTCCAGGGCCAAACATGAATCCTATTGCTCTTGGGAGCCGCTGGCTTGCTTATGCAGAAAACAAGTTGATTCGATGTCATCAGTCCCGT
GGTGGAGCCTGTGGAGACAACATTCAGTCTTATACTGCCACAGTCATTAGTGCTGCTAAAACATTGAAAAGTGGCCTGACAATGGTAGGG
AAAGTGGTGACTCAGCTGACAGGCACACTGCCTTCAGGTGTGACAGAAGATGATGTTGCCATCCACAGTAATTCACGGCGGAGTCCTTTG
GTCCCAGGCATCATCACAGTTATTGACACCGAAACCGTTGGAGAGGGCCAGGTGCTTGTGAGTGAGGATTCTGACAGTGATGGCATTGTG
GCCCACTTCCCTGCCCATGAGAAGCCAGTGTGCTGCATGGCTTTTAATACAAGTGGAATGCTTCTAGTCACAACAGACACCCTTGGCCAT
GACTTTCATGTCTTCCAAATTCTGACTCATCCTTGGTCCTCATCACAATGTGCTGTCCACCATCTGTATACTCTTCACAGGGGAGAAACT
GAAGCCAAAGTACAGGACATCTGCTTCAGCCATGACTGTCGCTGGGTTGTGGTCAGTACTCTCCGGGGTACTTCCCACGTTTTCCCCATC
AACCCTTATGGTGGCCAGCCTTGTGTTCGTACACATATGTCACCACGAGTAGTGAATCGCATGAGCCGTTTCCAGAAAAGTGCTGGACTG
GAAGAGATTGAACAAGAACTGACGTCTAAGCAAGGAGGTCGCTGTAGCCCTGTTCCAGGTCTATCAAGCAGCCCTTCTGGGTCACCCTTG
CATGGGAAACTGAACAGCCAAGACTCCTATAACAATTTTACCAACAACAACCCTGGCAACCCTCGGCTCTCTCCTCTTCCCAGCTTGATG
GTAGTGATGCCTCTTGCACAAATCAAGCAGCCAATGACATTGGGGACCATCACCAAACGAACCGGCAAAGTTAAACCTCCTCCACAAATT
TCACCCAGCAAATCGATGGGCGGAGAATTTTGTGTGGCTGCTATCTTCGGAACATCCAGGTCATGGTTTGCAAATAATGCAGGTCTGAAA
AGAGAAAAAGATCAGTCCAAACAAGTTGTAGTTGAGTCCCTGTACATTATCAGTTGCTATGGCACCTTAGTGGAACACATGATGGAGCCG
CGACCCCTCAGCACTGCACCCAAGATTAGTGACGACACACCACTGGAAATGATGACATCGCCTCGAGCCAGCTGGACTCTGGTTAGAACC
CCTCAATGGAATGAATTGCAGCCACCGTTTAATGCAAACCACCCTCTGCTCCTCGCTGCAGATGCAGTACAGTATTATCAGTTCCTGCTT
GCTGGCCGCATCAAGACAGAAGACAGCTTGAACCATTCCCCTGGCCAGAGTGGATTCCTCAGCTATGGCTCCAGCTTCAGCACCTCACCC
ACTGGACAGAGCCCATACACCTACCAGATGCACGGCACAACAGGGTTCTATCAAGGAGGAAATGGACTGGGCAACGCAGCCGGTTTCGGG
AGTGTGCACCAGGACTATCCTTCCTACCCCGGCTTCCCCCAGAGCCAGTACCCCCAGTATTACGGCTCATCCTACAACCCTCCCTACGTC
CCGGCCAGCAGCATCTGCCCTTCGCCCCTCTCCACGTCCACCTACGTCCTCCAGGAGGCATCTCACAACGTCCCCAACCAGAGTTCCGAG
TCACTTGCTGGTGAATACAACACACACAATGGACCTTCCACACCAGCGAAAGAGGGAGACACAGACAGGCCGCACCGGGCCTCCGACGGG
AAGCTCCGAGGCCGGTCTAAGAGGAGCAGTGACCCGTCCCCGGCAGGGGACAATGAGATTGAGCGTGTGTTCGTGTGGGACTTGGATGAG
ACAATAATTATTTTTCACTCCTTACTCACGGGGACATTTGCATCCAGATACGGGAAGGACACCACGACGTCCGTGCGCATTGGCCTTATG
ATGGAAGAGATGATCTTCAACCTTGCAGATACACATCTGTTCTTCAATGACCTGGAGGATTGTGACCAGATCCACGTTGATGACGTCTCA
TCAGATGACAATGGCCAAGATTTAAGCACATACAACTTCTCCGCTGACGGCTTCCACAGTTCGGCCCCAGGAGCCAACCTGTGCCTGGGC
TCTGGCGTGCACGGCGGCGTGGACTGGATGAGGAAGCTGGCCTTCCGCTACCGGCGGGTGAAGGAGATGTACAATACCTACAAGAACAAC
GTTGGTGGGTTGATAGGCACTCCCAAAAGGGAGACCTGGCTACAGCTCCGAGCTGAGCTGGAAGCTCTCACAGACCTCTGGCTGACCCAC
TCCCTGAAGGCACTAAACCTCATCAACTCCCGGCCCAACTGTGTCAATGTGCTGGTCACCACCACTCAACTAATTCCTGCCCTGGCCAAA
GTCCTGCTATATGGCCTGGGGTCTGTGTTTCCTATTGAGAACATCTACAGTGCAACCAAGACAGGGAAGGAGAGCTGCTTCGAGAGGATA
ATGCAGAGATTCGGCAGAAAAGCTGTCTACGTGGTGATCGGTGATGGTGTGGAAGAGGAGCAAGGAGCGAAAAAGCACAACATGCCTTTC
TGGCGGATATCCTGCCACGCAGACCTGGAGGCACTGAGGCACGCCCTGGAGCTGGAGTATTTATAGCAGGATCAGCAGCATCTCCACCTG
CCATCTCACCCTCAGACCCCCTCGCCTTCCCCACCTCCCCACCGAGAACTCCAGAGACCCAGATGTTGGACACCAGGAAGGGGCCCCACA
GCCGAGACGACGTGTCCAGTGACCATCTCAGAAGCCGTCCATCAGTCCAAATGGGGGTTCTGAGAAGGAAAGTACCCAACATTGGCTTCG
GAGTATTTGACTTTGGGGAAAAGGGCTGGCTCGGAGTCTAGACTCTTCTGTAAGACTCACAGAACAAAAGCAAGGAATTGCTGATTTGGG
GGGTGCCTGGTGATGAGGAGGGGATGGGTTTGTCTTGTCTTCTTTTTAATTTATGGACTAGTCTCATTACTCCGGAATTATGCTCTTGTA
CCTGTGTGGCTGGGTTTCTTAGTCGTTGGTTTGGTTTGGTTTTTTGAACTGGTATGTGGGGTGGTTCACAGTTCTAATGTAAGCACTCTA
TTCTCCAAGTTGTGCTTTGTGGGGACAATCATTCTTTGAACATTAGAGAGGAAGGCAGTTCAAGCTGTTGAAAAGACTATTGCTTATTTT
TGTTTTTAAAGACCTACTTGACGTCATGTGGACAGTGCACGTGCCTTACGCTACATCTTGTTTTCTAGGAAGAGGGGGATGCTGGGAAGG

>In-frame_ENST00000407086_ENST00000327619_TCGA-UL-AAZ6-01A_BCAS3_chr17_59118253_+_EYA2_chr20_45644820_length(amino acids)=1115AA_start in transcript=48_stop in transcript=3395
MNEAMATDSPRRPSRCTGGVVVRPQAVTEQSYMESVVTFLQDVVPQAYSGTPLTEEKEKIVWVRFENADLNDTSRNLEFHEIHSTGNEPP
LLIMIGYSDGMQVWSIPISGEAQELFSVRHGPIRAARILPAPQFGAQKCDNFAEKRPLLGVCKSIGSSGTSPPYCCVDLYSLRTGEMVKS
IQFKTPIYDLHCNKRILVVVLQEKIAAFDSCTFTKKFFVTSCYPCPGPNMNPIALGSRWLAYAENKLIRCHQSRGGACGDNIQSYTATVI
SAAKTLKSGLTMVGKVVTQLTGTLPSGVTEDDVAIHSNSRRSPLVPGIITVIDTETVGEGQVLVSEDSDSDGIVAHFPAHEKPVCCMAFN
TSGMLLVTTDTLGHDFHVFQILTHPWSSSQCAVHHLYTLHRGETEAKVQDICFSHDCRWVVVSTLRGTSHVFPINPYGGQPCVRTHMSPR
VVNRMSRFQKSAGLEEIEQELTSKQGGRCSPVPGLSSSPSGSPLHGKLNSQDSYNNFTNNNPGNPRLSPLPSLMVVMPLAQIKQPMTLGT
ITKRTGKVKPPPQISPSKSMGGEFCVAAIFGTSRSWFANNAGLKREKDQSKQVVVESLYIISCYGTLVEHMMEPRPLSTAPKISDDTPLE
MMTSPRASWTLVRTPQWNELQPPFNANHPLLLAADAVQYYQFLLAGRIKTEDSLNHSPGQSGFLSYGSSFSTSPTGQSPYTYQMHGTTGF
YQGGNGLGNAAGFGSVHQDYPSYPGFPQSQYPQYYGSSYNPPYVPASSICPSPLSTSTYVLQEASHNVPNQSSESLAGEYNTHNGPSTPA
KEGDTDRPHRASDGKLRGRSKRSSDPSPAGDNEIERVFVWDLDETIIIFHSLLTGTFASRYGKDTTTSVRIGLMMEEMIFNLADTHLFFN
DLEDCDQIHVDDVSSDDNGQDLSTYNFSADGFHSSAPGANLCLGSGVHGGVDWMRKLAFRYRRVKEMYNTYKNNVGGLIGTPKRETWLQL
RAELEALTDLWLTHSLKALNLINSRPNCVNVLVTTTQLIPALAKVLLYGLGSVFPIENIYSATKTGKESCFERIMQRFGRKAVYVVIGDG

--------------------------------------------------------------
>In-frame_ENST00000390652_ENST00000357410_TCGA-UL-AAZ6-01A_BCAS3_chr17_59118253_+_EYA2_chr20_45644820_length(transcript)=3898nt_BP=2105nt
ACTAGCGTTAACCGGCGGGGCGGCCGGTTTTATGAATGAAGCTATGGCTACAGATTCCCCAAGAAGACCCAGTCGTTGTACTGGTGGAGT
TGTGGTTCGCCCCCAGGCTGTCACAGAGCAGTCCTACATGGAAAGTGTTGTGACTTTTCTGCAGGATGTTGTGCCACAGGCTTACAGTGG
AACACCTCTAACAGAAGAAAAGGAGAAAATAGTCTGGGTCAGATTTGAAAATGCAGATTTAAATGATACATCAAGAAATCTGGAATTTCA
TGAAATACATAGTACTGGGAATGAACCGCCTTTGTTGATTATGATTGGCTACAGTGATGGAATGCAGGTCTGGAGCATCCCTATCAGTGG
TGAAGCACAAGAGCTCTTCTCTGTTCGACATGGCCCAATTCGAGCGGCTAGAATCTTGCCTGCTCCACAGTTTGGTGCTCAAAAATGTGA
TAACTTTGCTGAAAAAAGACCCCTCCTTGGTGTTTGTAAGAGCATTGGATCTTCTGGCACAAGCCCACCGTACTGTTGTGTGGATCTGTA
TTCACTTCGTACTGGGGAGATGGTCAAGTCCATTCAATTTAAGACACCTATTTATGATCTCCATTGCAATAAACGGATCCTTGTCGTAGT
CTTGCAGGAGAAAATTGCTGCCTTTGATAGCTGTACTTTCACGAAGAAATTCTTTGTTACAAGCTGCTATCCATGTCCAGGGCCAAACAT
GAATCCTATTGCTCTTGGGAGCCGCTGGCTTGCTTATGCAGAAAACAAGTTGATTCGATGTCATCAGTCCCGTGGTGGAGCCTGTGGAGA
CAACATTCAGTCTTATACTGCCACAGTCATTAGTGCTGCTAAAACATTGAAAAGTGGCCTGACAATGGTAGGGAAAGTGGTGACTCAGCT
GACAGGCACACTGCCTTCAGGTGTGACAGAAGATGATGTTGCCATCCACAGTAATTCACGGCGGAGTCCTTTGGTCCCAGGCATCATCAC
AGTTATTGACACCGAAACCGTTGGAGAGGGCCAGGTGCTTGTGAGTGAGGATTCTGACAGTGATGGCATTGTGGCCCACTTCCCTGCCCA
TGAGAAGCCAGTGTGCTGCATGGCTTTTAATACAAGTGGAATGCTTCTAGTCACAACAGACACCCTTGGCCATGACTTTCATGTCTTCCA
AATTCTGACTCATCCTTGGTCCTCATCACAATGTGCTGTCCACCATCTGTATACTCTTCACAGGGGAGAAACTGAAGCCAAAGTACAGGA
CATCTGCTTCAGCCATGACTGTCGCTGGGTTGTGGTCAGTACTCTCCGGGGTACTTCCCACGTTTTCCCCATCAACCCTTATGGTGGCCA
GCCTTGTGTTCGTACACATATGTCACCACGAGTAGTGAATCGCATGAGCCGTTTCCAGAAAAGTGCTGGACTGGAAGAGATTGAACAAGA
ACTGACGTCTAAGCAAGGAGGTCGCTGTAGCCCTGTTCCAGGTCTATCAAGCAGCCCTTCTGGGTCACCCTTGCATGGGAAACTGAACAG
CCAAGACTCCTATAACAATTTTACCAACAACAACCCTGGCAACCCTCGGCTCTCTCCTCTTCCCAGCTTGATGGTAGTGATGCCTCTTGC
ACAAATCAAGCAGCCAATGACATTGGGGACCATCACCAAACGAACCGGGCCTTATCTCTTTGGAGCGGGGTGTTTTTCCATAAAAGCCCC
ATGCAAAGTTAAACCTCCTCCACAAATTTCACCCAGCAAATCGATGGGCGGAGAATTTTGTGTGGCTGCTATCTTCGGAACATCCAGGTC
ATGGTTTGCAAATAATGCAGGTCTGAAAAGAGAAAAAGATCAGTCCAAACAAGTTGTAGTTGAGTCCCTGTACATTATCAGTTGCTATGG
CACCTTAGTGGAACACATGATGGAGCCGCGACCCCTCAGCACTGCACCCAAGATTAGTGACGACACACCACTGGAAATGATGACATCGCC
TCGAGCCAGCTGGACTCTGGTTAGAACCCCTCAATGGAATGAATTGCAGCCACCGTTTAATGCAAACCACCCTCTGCTCCTCGCTGCAGA
TGCAGTACAGTATTATCAGTTCCTGCTTGCTGGCCGCATCAAGACAGAAGACAGCTTGAACCATTCCCCTGGCCAGAGTGGATTCCTCAG
CTATGGCTCCAGCTTCAGCACCTCACCCACTGGACAGAGCCCATACACCTACCAGATGCACGGCACAACAGGGTTCTATCAAGGAGGAAA
TGGACTGGGCAACGCAGCCGGTTTCGGGAGTGTGCACCAGGACTATCCTTCCTACCCCGGCTTCCCCCAGAGCCAGTACCCCCAGTATTA
CGGCTCATCCTACAACCCTCCCTACGTCCCGGCCAGCAGCATCTGCCCTTCGCCCCTCTCCACGTCCACCTACGTCCTCCAGGAGGCATC
TCACAACGTCCCCAACCAGAGTTCCGAGTCACTTGCTGGTGAATACAACACACACAATGGACCTTCCACACCAGCGAAAGAGGGAGACAC
AGACAGGCCGCACCGGGCCTCCGACGGGAAGCTCCGAGGCCGGTCTAAGAGGAGCAGTGACCCGTCCCCGGCAGGGGACAATGAGATTGA
GCGTGTGTTCGTGTGGGACTTGGATGAGACAATAATTATTTTTCACTCCTTACTCACGGGGACATTTGCATCCAGATACGGGAAGGACAC
CACGACGTCCGTGCGCATTGGCCTTATGATGGAAGAGATGATCTTCAACCTTGCAGATACACATCTGTTCTTCAATGACCTGGAGGATTG
TGACCAGATCCACGTTGATGACGTCTCATCAGATGACAATGGCCAAGATTTAAGCACATACAACTTCTCCGCTGACGGCTTCCACAGTTC
GGCCCCAGGAGCCAACCTGTGCCTGGGCTCTGGCGTGCACGGCGGCGTGGACTGGATGAGGAAGCTGGCCTTCCGCTACCGGCGGGTGAA
GGAGATGTACAATACCTACAAGAACAACGTTGGTGGGAAGGAGAGCTGCTTCGAGAGGATAATGCAGAGATTCGGCAGAAAAGCTGTCTA
CGTGGTGATCGGTGATGGTGTGGAAGAGGAGCAAGGAGCGAAAAAGCACAACATGCCTTTCTGGCGGATATCCTGCCACGCAGACCTGGA
GGCACTGAGGCACGCCCTGGAGCTGGAGTATTTATAGCAGGATCAGCAGCATCTCCACCTGCCATCTCACCCTCAGACCCCCTCGCCTTC
CCCACCTCCCCACCGAGAACTCCAGAGACCCAGATGTTGGACACCAGGAAGGGGCCCCACAGCCGAGACGACGTGTCCAGTGACCATCTC
AGAAGCCGTCCATCAGTCCAAATGGGGGTTCTGAGAAGGAAAGTACCCAACATTGGCTTCGGAGTATTTGACTTTGGGGAAAAGGGCTGG
CTCGGAGTCTAGACTCTTCTGTAAGACTCACAGAACAAAAGCAAGGAATTGCTGATTTGGGGGGTGCCTGGTGATGAGGAGGGGATGGGT
TTGTCTTGTCTTCTTTTTAATTTATGGACTAGTCTCATTACTCCGGAATTATGCTCTTGTACCTGTGTGGCTGGGTTTCTTAGTCGTTGG
TTTGGTTTGGTTTTTTGAACTGGTATGTGGGGTGGTTCACAGTTCTAATGTAAGCACTCTATTCTCCAAGTTGTGCTTTGTGGGGACAAT
CATTCTTTGAACATTAGAGAGGAAGGCAGTTCAAGCTGTTGAAAAGACTATTGCTTATTTTTGTTTTTAAAGACCTACTTGACGTCATGT
GGACAGTGCACGTGCCTTACGCTACATCTTGTTTTCTAGGAAGAGGGGGATGCTGGGAAGGAATGGGTGCTTTGTGATGGATAAAAGGCA

>In-frame_ENST00000390652_ENST00000357410_TCGA-UL-AAZ6-01A_BCAS3_chr17_59118253_+_EYA2_chr20_45644820_length(amino acids)=1051AA_start in transcript=31_stop in transcript=3186
MNEAMATDSPRRPSRCTGGVVVRPQAVTEQSYMESVVTFLQDVVPQAYSGTPLTEEKEKIVWVRFENADLNDTSRNLEFHEIHSTGNEPP
LLIMIGYSDGMQVWSIPISGEAQELFSVRHGPIRAARILPAPQFGAQKCDNFAEKRPLLGVCKSIGSSGTSPPYCCVDLYSLRTGEMVKS
IQFKTPIYDLHCNKRILVVVLQEKIAAFDSCTFTKKFFVTSCYPCPGPNMNPIALGSRWLAYAENKLIRCHQSRGGACGDNIQSYTATVI
SAAKTLKSGLTMVGKVVTQLTGTLPSGVTEDDVAIHSNSRRSPLVPGIITVIDTETVGEGQVLVSEDSDSDGIVAHFPAHEKPVCCMAFN
TSGMLLVTTDTLGHDFHVFQILTHPWSSSQCAVHHLYTLHRGETEAKVQDICFSHDCRWVVVSTLRGTSHVFPINPYGGQPCVRTHMSPR
VVNRMSRFQKSAGLEEIEQELTSKQGGRCSPVPGLSSSPSGSPLHGKLNSQDSYNNFTNNNPGNPRLSPLPSLMVVMPLAQIKQPMTLGT
ITKRTGPYLFGAGCFSIKAPCKVKPPPQISPSKSMGGEFCVAAIFGTSRSWFANNAGLKREKDQSKQVVVESLYIISCYGTLVEHMMEPR
PLSTAPKISDDTPLEMMTSPRASWTLVRTPQWNELQPPFNANHPLLLAADAVQYYQFLLAGRIKTEDSLNHSPGQSGFLSYGSSFSTSPT
GQSPYTYQMHGTTGFYQGGNGLGNAAGFGSVHQDYPSYPGFPQSQYPQYYGSSYNPPYVPASSICPSPLSTSTYVLQEASHNVPNQSSES
LAGEYNTHNGPSTPAKEGDTDRPHRASDGKLRGRSKRSSDPSPAGDNEIERVFVWDLDETIIIFHSLLTGTFASRYGKDTTTSVRIGLMM
EEMIFNLADTHLFFNDLEDCDQIHVDDVSSDDNGQDLSTYNFSADGFHSSAPGANLCLGSGVHGGVDWMRKLAFRYRRVKEMYNTYKNNV

--------------------------------------------------------------
>In-frame_ENST00000390652_ENST00000327619_TCGA-UL-AAZ6-01A_BCAS3_chr17_59118253_+_EYA2_chr20_45644820_length(transcript)=4135nt_BP=2105nt
ACTAGCGTTAACCGGCGGGGCGGCCGGTTTTATGAATGAAGCTATGGCTACAGATTCCCCAAGAAGACCCAGTCGTTGTACTGGTGGAGT
TGTGGTTCGCCCCCAGGCTGTCACAGAGCAGTCCTACATGGAAAGTGTTGTGACTTTTCTGCAGGATGTTGTGCCACAGGCTTACAGTGG
AACACCTCTAACAGAAGAAAAGGAGAAAATAGTCTGGGTCAGATTTGAAAATGCAGATTTAAATGATACATCAAGAAATCTGGAATTTCA
TGAAATACATAGTACTGGGAATGAACCGCCTTTGTTGATTATGATTGGCTACAGTGATGGAATGCAGGTCTGGAGCATCCCTATCAGTGG
TGAAGCACAAGAGCTCTTCTCTGTTCGACATGGCCCAATTCGAGCGGCTAGAATCTTGCCTGCTCCACAGTTTGGTGCTCAAAAATGTGA
TAACTTTGCTGAAAAAAGACCCCTCCTTGGTGTTTGTAAGAGCATTGGATCTTCTGGCACAAGCCCACCGTACTGTTGTGTGGATCTGTA
TTCACTTCGTACTGGGGAGATGGTCAAGTCCATTCAATTTAAGACACCTATTTATGATCTCCATTGCAATAAACGGATCCTTGTCGTAGT
CTTGCAGGAGAAAATTGCTGCCTTTGATAGCTGTACTTTCACGAAGAAATTCTTTGTTACAAGCTGCTATCCATGTCCAGGGCCAAACAT
GAATCCTATTGCTCTTGGGAGCCGCTGGCTTGCTTATGCAGAAAACAAGTTGATTCGATGTCATCAGTCCCGTGGTGGAGCCTGTGGAGA
CAACATTCAGTCTTATACTGCCACAGTCATTAGTGCTGCTAAAACATTGAAAAGTGGCCTGACAATGGTAGGGAAAGTGGTGACTCAGCT
GACAGGCACACTGCCTTCAGGTGTGACAGAAGATGATGTTGCCATCCACAGTAATTCACGGCGGAGTCCTTTGGTCCCAGGCATCATCAC
AGTTATTGACACCGAAACCGTTGGAGAGGGCCAGGTGCTTGTGAGTGAGGATTCTGACAGTGATGGCATTGTGGCCCACTTCCCTGCCCA
TGAGAAGCCAGTGTGCTGCATGGCTTTTAATACAAGTGGAATGCTTCTAGTCACAACAGACACCCTTGGCCATGACTTTCATGTCTTCCA
AATTCTGACTCATCCTTGGTCCTCATCACAATGTGCTGTCCACCATCTGTATACTCTTCACAGGGGAGAAACTGAAGCCAAAGTACAGGA
CATCTGCTTCAGCCATGACTGTCGCTGGGTTGTGGTCAGTACTCTCCGGGGTACTTCCCACGTTTTCCCCATCAACCCTTATGGTGGCCA
GCCTTGTGTTCGTACACATATGTCACCACGAGTAGTGAATCGCATGAGCCGTTTCCAGAAAAGTGCTGGACTGGAAGAGATTGAACAAGA
ACTGACGTCTAAGCAAGGAGGTCGCTGTAGCCCTGTTCCAGGTCTATCAAGCAGCCCTTCTGGGTCACCCTTGCATGGGAAACTGAACAG
CCAAGACTCCTATAACAATTTTACCAACAACAACCCTGGCAACCCTCGGCTCTCTCCTCTTCCCAGCTTGATGGTAGTGATGCCTCTTGC
ACAAATCAAGCAGCCAATGACATTGGGGACCATCACCAAACGAACCGGGCCTTATCTCTTTGGAGCGGGGTGTTTTTCCATAAAAGCCCC
ATGCAAAGTTAAACCTCCTCCACAAATTTCACCCAGCAAATCGATGGGCGGAGAATTTTGTGTGGCTGCTATCTTCGGAACATCCAGGTC
ATGGTTTGCAAATAATGCAGGTCTGAAAAGAGAAAAAGATCAGTCCAAACAAGTTGTAGTTGAGTCCCTGTACATTATCAGTTGCTATGG
CACCTTAGTGGAACACATGATGGAGCCGCGACCCCTCAGCACTGCACCCAAGATTAGTGACGACACACCACTGGAAATGATGACATCGCC
TCGAGCCAGCTGGACTCTGGTTAGAACCCCTCAATGGAATGAATTGCAGCCACCGTTTAATGCAAACCACCCTCTGCTCCTCGCTGCAGA
TGCAGTACAGTATTATCAGTTCCTGCTTGCTGGCCGCATCAAGACAGAAGACAGCTTGAACCATTCCCCTGGCCAGAGTGGATTCCTCAG
CTATGGCTCCAGCTTCAGCACCTCACCCACTGGACAGAGCCCATACACCTACCAGATGCACGGCACAACAGGGTTCTATCAAGGAGGAAA
TGGACTGGGCAACGCAGCCGGTTTCGGGAGTGTGCACCAGGACTATCCTTCCTACCCCGGCTTCCCCCAGAGCCAGTACCCCCAGTATTA
CGGCTCATCCTACAACCCTCCCTACGTCCCGGCCAGCAGCATCTGCCCTTCGCCCCTCTCCACGTCCACCTACGTCCTCCAGGAGGCATC
TCACAACGTCCCCAACCAGAGTTCCGAGTCACTTGCTGGTGAATACAACACACACAATGGACCTTCCACACCAGCGAAAGAGGGAGACAC
AGACAGGCCGCACCGGGCCTCCGACGGGAAGCTCCGAGGCCGGTCTAAGAGGAGCAGTGACCCGTCCCCGGCAGGGGACAATGAGATTGA
GCGTGTGTTCGTGTGGGACTTGGATGAGACAATAATTATTTTTCACTCCTTACTCACGGGGACATTTGCATCCAGATACGGGAAGGACAC
CACGACGTCCGTGCGCATTGGCCTTATGATGGAAGAGATGATCTTCAACCTTGCAGATACACATCTGTTCTTCAATGACCTGGAGGATTG
TGACCAGATCCACGTTGATGACGTCTCATCAGATGACAATGGCCAAGATTTAAGCACATACAACTTCTCCGCTGACGGCTTCCACAGTTC
GGCCCCAGGAGCCAACCTGTGCCTGGGCTCTGGCGTGCACGGCGGCGTGGACTGGATGAGGAAGCTGGCCTTCCGCTACCGGCGGGTGAA
GGAGATGTACAATACCTACAAGAACAACGTTGGTGGGTTGATAGGCACTCCCAAAAGGGAGACCTGGCTACAGCTCCGAGCTGAGCTGGA
AGCTCTCACAGACCTCTGGCTGACCCACTCCCTGAAGGCACTAAACCTCATCAACTCCCGGCCCAACTGTGTCAATGTGCTGGTCACCAC
CACTCAACTAATTCCTGCCCTGGCCAAAGTCCTGCTATATGGCCTGGGGTCTGTGTTTCCTATTGAGAACATCTACAGTGCAACCAAGAC
AGGGAAGGAGAGCTGCTTCGAGAGGATAATGCAGAGATTCGGCAGAAAAGCTGTCTACGTGGTGATCGGTGATGGTGTGGAAGAGGAGCA
AGGAGCGAAAAAGCACAACATGCCTTTCTGGCGGATATCCTGCCACGCAGACCTGGAGGCACTGAGGCACGCCCTGGAGCTGGAGTATTT
ATAGCAGGATCAGCAGCATCTCCACCTGCCATCTCACCCTCAGACCCCCTCGCCTTCCCCACCTCCCCACCGAGAACTCCAGAGACCCAG
ATGTTGGACACCAGGAAGGGGCCCCACAGCCGAGACGACGTGTCCAGTGACCATCTCAGAAGCCGTCCATCAGTCCAAATGGGGGTTCTG
AGAAGGAAAGTACCCAACATTGGCTTCGGAGTATTTGACTTTGGGGAAAAGGGCTGGCTCGGAGTCTAGACTCTTCTGTAAGACTCACAG
AACAAAAGCAAGGAATTGCTGATTTGGGGGGTGCCTGGTGATGAGGAGGGGATGGGTTTGTCTTGTCTTCTTTTTAATTTATGGACTAGT
CTCATTACTCCGGAATTATGCTCTTGTACCTGTGTGGCTGGGTTTCTTAGTCGTTGGTTTGGTTTGGTTTTTTGAACTGGTATGTGGGGT
GGTTCACAGTTCTAATGTAAGCACTCTATTCTCCAAGTTGTGCTTTGTGGGGACAATCATTCTTTGAACATTAGAGAGGAAGGCAGTTCA
AGCTGTTGAAAAGACTATTGCTTATTTTTGTTTTTAAAGACCTACTTGACGTCATGTGGACAGTGCACGTGCCTTACGCTACATCTTGTT

>In-frame_ENST00000390652_ENST00000327619_TCGA-UL-AAZ6-01A_BCAS3_chr17_59118253_+_EYA2_chr20_45644820_length(amino acids)=1130AA_start in transcript=31_stop in transcript=3423
MNEAMATDSPRRPSRCTGGVVVRPQAVTEQSYMESVVTFLQDVVPQAYSGTPLTEEKEKIVWVRFENADLNDTSRNLEFHEIHSTGNEPP
LLIMIGYSDGMQVWSIPISGEAQELFSVRHGPIRAARILPAPQFGAQKCDNFAEKRPLLGVCKSIGSSGTSPPYCCVDLYSLRTGEMVKS
IQFKTPIYDLHCNKRILVVVLQEKIAAFDSCTFTKKFFVTSCYPCPGPNMNPIALGSRWLAYAENKLIRCHQSRGGACGDNIQSYTATVI
SAAKTLKSGLTMVGKVVTQLTGTLPSGVTEDDVAIHSNSRRSPLVPGIITVIDTETVGEGQVLVSEDSDSDGIVAHFPAHEKPVCCMAFN
TSGMLLVTTDTLGHDFHVFQILTHPWSSSQCAVHHLYTLHRGETEAKVQDICFSHDCRWVVVSTLRGTSHVFPINPYGGQPCVRTHMSPR
VVNRMSRFQKSAGLEEIEQELTSKQGGRCSPVPGLSSSPSGSPLHGKLNSQDSYNNFTNNNPGNPRLSPLPSLMVVMPLAQIKQPMTLGT
ITKRTGPYLFGAGCFSIKAPCKVKPPPQISPSKSMGGEFCVAAIFGTSRSWFANNAGLKREKDQSKQVVVESLYIISCYGTLVEHMMEPR
PLSTAPKISDDTPLEMMTSPRASWTLVRTPQWNELQPPFNANHPLLLAADAVQYYQFLLAGRIKTEDSLNHSPGQSGFLSYGSSFSTSPT
GQSPYTYQMHGTTGFYQGGNGLGNAAGFGSVHQDYPSYPGFPQSQYPQYYGSSYNPPYVPASSICPSPLSTSTYVLQEASHNVPNQSSES
LAGEYNTHNGPSTPAKEGDTDRPHRASDGKLRGRSKRSSDPSPAGDNEIERVFVWDLDETIIIFHSLLTGTFASRYGKDTTTSVRIGLMM
EEMIFNLADTHLFFNDLEDCDQIHVDDVSSDDNGQDLSTYNFSADGFHSSAPGANLCLGSGVHGGVDWMRKLAFRYRRVKEMYNTYKNNV
GGLIGTPKRETWLQLRAELEALTDLWLTHSLKALNLINSRPNCVNVLVTTTQLIPALAKVLLYGLGSVFPIENIYSATKTGKESCFERIM

--------------------------------------------------------------
>In-frame_ENST00000408905_ENST00000357410_TCGA-UL-AAZ6-01A_BCAS3_chr17_59118253_+_EYA2_chr20_45644820_length(transcript)=3822nt_BP=2029nt
ATGAATGAAGCTATGGCTACAGATTCCCCAAGAAGACCCAGTCGTTGTACTGGTGGAGTTGTGGTTCGCCCCCAGGCTGTCACAGAGCAG
TCCTACATGGAAAGTGTTGTGACTTTTCTGCAGGATGTTGTGCCACAGGCTTACAGTGGAACACCTCTAACAGAAGAAAAGGAGAAAATA
GTCTGGGTCAGATTTGAAAATGCAGATTTAAATGATACATCAAGAAATCTGGAATTTCATGAAATACATAGTACTGGGAATGAACCGCCT
TTGTTGATTATGATTGGCTACAGTGATGGAATGCAGGTCTGGAGCATCCCTATCAGTGGTGAAGCACAAGAGCTCTTCTCTGTTCGACAT
GGCCCAATTCGAGCGGCTAGAATCTTGCCTGCTCCACAGTTTGGTGCTCAAAAATGTGATAACTTTGCTGAAAAAAGACCCCTCCTTGGT
GTTTGTAAGAGCATTGGATCTTCTGGCACAAGCCCACCGTACTGTTGTGTGGATCTGTATTCACTTCGTACTGGGGAGATGGTCAAGTCC
ATTCAATTTAAGACACCTATTTATGATCTCCATTGCAATAAACGGATCCTTGTCGTAGTCTTGCAGGAGAAAATTGCTGCCTTTGATAGC
TGTACTTTCACGAAGAAATTCTTTGTTACAAGCTGCTATCCATGTCCAGGGCCAAACATGAATCCTATTGCTCTTGGGAGCCGCTGGCTT
GCTTATGCAGAAAACAAGTTGATTCGATGTCATCAGTCCCGTGGTGGAGCCTGTGGAGACAACATTCAGTCTTATACTGCCACAGTCATT
AGTGCTGCTAAAACATTGAAAAGTGGCCTGACAATGGTAGGGAAAGTGGTGACTCAGCTGACAGGCACACTGCCTTCAGGTGTGACAGAA
GATGATGTTGCCATCCACAGTAATTCACGGCGGAGTCCTTTGGTCCCAGGCATCATCACAGTTATTGACACCGAAACCGTTGGAGAGGGC
CAGGTGCTTGTGAGTGAGGATTCTGACAGTGATGGCATTGTGGCCCACTTCCCTGCCCATGAGAAGCCAGTGTGCTGCATGGCTTTTAAT
ACAAGTGGAATGCTTCTAGTCACAACAGACACCCTTGGCCATGACTTTCATGTCTTCCAAATTCTGACTCATCCTTGGTCCTCATCACAA
TGTGCTGTCCACCATCTGTATACTCTTCACAGGGGAGAAACTGAAGCCAAAGTACAGGACATCTGCTTCAGCCATGACTGTCGCTGGGTT
GTGGTCAGTACTCTCCGGGGTACTTCCCACGTTTTCCCCATCAACCCTTATGGTGGCCAGCCTTGTGTTCGTACACATATGTCACCACGA
GTAGTGAATCGCATGAGCCGTTTCCAGAAAAGTGCTGGACTGGAAGAGATTGAACAAGAACTGACGTCTAAGCAAGGAGGTCGCTGTAGC
CCTGTTCCAGGTCTATCAAGCAGCCCTTCTGGGTCACCCTTGCATGGGAAACTGAACAGCCAAGACTCCTATAACAATTTTACCAACAAC
AACCCTGGCAACCCTCGGCTCTCTCCTCTTCCCAGCTTGATGGTAGTGATGCCTCTTGCACAAATCAAGCAGCCAATGACATTGGGGACC
ATCACCAAACGAACCGGCAAAGTTAAACCTCCTCCACAAATTTCACCCAGCAAATCGATGGGCGGAGAATTTTGTGTGGCTGCTATCTTC
GGAACATCCAGGTCATGGTTTGCAAATAATGCAGGTCTGAAAAGAGAAAAAGATCAGTCCAAACAAGTTGTAGTTGAGTCCCTGTACATT
ATCAGTTGCTATGGCACCTTAGTGGAACACATGATGGAGCCGCGACCCCTCAGCACTGCACCCAAGATTAGTGACGACACACCACTGGAA
ATGATGACATCGCCTCGAGCCAGCTGGACTCTGGTTAGAACCCCTCAATGGAATGAATTGCAGCCACCGTTTAATGCAAACCACCCTCTG
CTCCTCGCTGCAGATGCAGTACAGTATTATCAGTTCCTGCTTGCTGGCCGCATCAAGACAGAAGACAGCTTGAACCATTCCCCTGGCCAG
AGTGGATTCCTCAGCTATGGCTCCAGCTTCAGCACCTCACCCACTGGACAGAGCCCATACACCTACCAGATGCACGGCACAACAGGGTTC
TATCAAGGAGGAAATGGACTGGGCAACGCAGCCGGTTTCGGGAGTGTGCACCAGGACTATCCTTCCTACCCCGGCTTCCCCCAGAGCCAG
TACCCCCAGTATTACGGCTCATCCTACAACCCTCCCTACGTCCCGGCCAGCAGCATCTGCCCTTCGCCCCTCTCCACGTCCACCTACGTC
CTCCAGGAGGCATCTCACAACGTCCCCAACCAGAGTTCCGAGTCACTTGCTGGTGAATACAACACACACAATGGACCTTCCACACCAGCG
AAAGAGGGAGACACAGACAGGCCGCACCGGGCCTCCGACGGGAAGCTCCGAGGCCGGTCTAAGAGGAGCAGTGACCCGTCCCCGGCAGGG
GACAATGAGATTGAGCGTGTGTTCGTGTGGGACTTGGATGAGACAATAATTATTTTTCACTCCTTACTCACGGGGACATTTGCATCCAGA
TACGGGAAGGACACCACGACGTCCGTGCGCATTGGCCTTATGATGGAAGAGATGATCTTCAACCTTGCAGATACACATCTGTTCTTCAAT
GACCTGGAGGATTGTGACCAGATCCACGTTGATGACGTCTCATCAGATGACAATGGCCAAGATTTAAGCACATACAACTTCTCCGCTGAC
GGCTTCCACAGTTCGGCCCCAGGAGCCAACCTGTGCCTGGGCTCTGGCGTGCACGGCGGCGTGGACTGGATGAGGAAGCTGGCCTTCCGC
TACCGGCGGGTGAAGGAGATGTACAATACCTACAAGAACAACGTTGGTGGGAAGGAGAGCTGCTTCGAGAGGATAATGCAGAGATTCGGC
AGAAAAGCTGTCTACGTGGTGATCGGTGATGGTGTGGAAGAGGAGCAAGGAGCGAAAAAGCACAACATGCCTTTCTGGCGGATATCCTGC
CACGCAGACCTGGAGGCACTGAGGCACGCCCTGGAGCTGGAGTATTTATAGCAGGATCAGCAGCATCTCCACCTGCCATCTCACCCTCAG
ACCCCCTCGCCTTCCCCACCTCCCCACCGAGAACTCCAGAGACCCAGATGTTGGACACCAGGAAGGGGCCCCACAGCCGAGACGACGTGT
CCAGTGACCATCTCAGAAGCCGTCCATCAGTCCAAATGGGGGTTCTGAGAAGGAAAGTACCCAACATTGGCTTCGGAGTATTTGACTTTG
GGGAAAAGGGCTGGCTCGGAGTCTAGACTCTTCTGTAAGACTCACAGAACAAAAGCAAGGAATTGCTGATTTGGGGGGTGCCTGGTGATG
AGGAGGGGATGGGTTTGTCTTGTCTTCTTTTTAATTTATGGACTAGTCTCATTACTCCGGAATTATGCTCTTGTACCTGTGTGGCTGGGT
TTCTTAGTCGTTGGTTTGGTTTGGTTTTTTGAACTGGTATGTGGGGTGGTTCACAGTTCTAATGTAAGCACTCTATTCTCCAAGTTGTGC
TTTGTGGGGACAATCATTCTTTGAACATTAGAGAGGAAGGCAGTTCAAGCTGTTGAAAAGACTATTGCTTATTTTTGTTTTTAAAGACCT
ACTTGACGTCATGTGGACAGTGCACGTGCCTTACGCTACATCTTGTTTTCTAGGAAGAGGGGGATGCTGGGAAGGAATGGGTGCTTTGTG

>In-frame_ENST00000408905_ENST00000357410_TCGA-UL-AAZ6-01A_BCAS3_chr17_59118253_+_EYA2_chr20_45644820_length(amino acids)=1036AA_start in transcript=0_stop in transcript=3110
MNEAMATDSPRRPSRCTGGVVVRPQAVTEQSYMESVVTFLQDVVPQAYSGTPLTEEKEKIVWVRFENADLNDTSRNLEFHEIHSTGNEPP
LLIMIGYSDGMQVWSIPISGEAQELFSVRHGPIRAARILPAPQFGAQKCDNFAEKRPLLGVCKSIGSSGTSPPYCCVDLYSLRTGEMVKS
IQFKTPIYDLHCNKRILVVVLQEKIAAFDSCTFTKKFFVTSCYPCPGPNMNPIALGSRWLAYAENKLIRCHQSRGGACGDNIQSYTATVI
SAAKTLKSGLTMVGKVVTQLTGTLPSGVTEDDVAIHSNSRRSPLVPGIITVIDTETVGEGQVLVSEDSDSDGIVAHFPAHEKPVCCMAFN
TSGMLLVTTDTLGHDFHVFQILTHPWSSSQCAVHHLYTLHRGETEAKVQDICFSHDCRWVVVSTLRGTSHVFPINPYGGQPCVRTHMSPR
VVNRMSRFQKSAGLEEIEQELTSKQGGRCSPVPGLSSSPSGSPLHGKLNSQDSYNNFTNNNPGNPRLSPLPSLMVVMPLAQIKQPMTLGT
ITKRTGKVKPPPQISPSKSMGGEFCVAAIFGTSRSWFANNAGLKREKDQSKQVVVESLYIISCYGTLVEHMMEPRPLSTAPKISDDTPLE
MMTSPRASWTLVRTPQWNELQPPFNANHPLLLAADAVQYYQFLLAGRIKTEDSLNHSPGQSGFLSYGSSFSTSPTGQSPYTYQMHGTTGF
YQGGNGLGNAAGFGSVHQDYPSYPGFPQSQYPQYYGSSYNPPYVPASSICPSPLSTSTYVLQEASHNVPNQSSESLAGEYNTHNGPSTPA
KEGDTDRPHRASDGKLRGRSKRSSDPSPAGDNEIERVFVWDLDETIIIFHSLLTGTFASRYGKDTTTSVRIGLMMEEMIFNLADTHLFFN
DLEDCDQIHVDDVSSDDNGQDLSTYNFSADGFHSSAPGANLCLGSGVHGGVDWMRKLAFRYRRVKEMYNTYKNNVGGKESCFERIMQRFG

--------------------------------------------------------------
>In-frame_ENST00000408905_ENST00000327619_TCGA-UL-AAZ6-01A_BCAS3_chr17_59118253_+_EYA2_chr20_45644820_length(transcript)=4059nt_BP=2029nt
ATGAATGAAGCTATGGCTACAGATTCCCCAAGAAGACCCAGTCGTTGTACTGGTGGAGTTGTGGTTCGCCCCCAGGCTGTCACAGAGCAG
TCCTACATGGAAAGTGTTGTGACTTTTCTGCAGGATGTTGTGCCACAGGCTTACAGTGGAACACCTCTAACAGAAGAAAAGGAGAAAATA
GTCTGGGTCAGATTTGAAAATGCAGATTTAAATGATACATCAAGAAATCTGGAATTTCATGAAATACATAGTACTGGGAATGAACCGCCT
TTGTTGATTATGATTGGCTACAGTGATGGAATGCAGGTCTGGAGCATCCCTATCAGTGGTGAAGCACAAGAGCTCTTCTCTGTTCGACAT
GGCCCAATTCGAGCGGCTAGAATCTTGCCTGCTCCACAGTTTGGTGCTCAAAAATGTGATAACTTTGCTGAAAAAAGACCCCTCCTTGGT
GTTTGTAAGAGCATTGGATCTTCTGGCACAAGCCCACCGTACTGTTGTGTGGATCTGTATTCACTTCGTACTGGGGAGATGGTCAAGTCC
ATTCAATTTAAGACACCTATTTATGATCTCCATTGCAATAAACGGATCCTTGTCGTAGTCTTGCAGGAGAAAATTGCTGCCTTTGATAGC
TGTACTTTCACGAAGAAATTCTTTGTTACAAGCTGCTATCCATGTCCAGGGCCAAACATGAATCCTATTGCTCTTGGGAGCCGCTGGCTT
GCTTATGCAGAAAACAAGTTGATTCGATGTCATCAGTCCCGTGGTGGAGCCTGTGGAGACAACATTCAGTCTTATACTGCCACAGTCATT
AGTGCTGCTAAAACATTGAAAAGTGGCCTGACAATGGTAGGGAAAGTGGTGACTCAGCTGACAGGCACACTGCCTTCAGGTGTGACAGAA
GATGATGTTGCCATCCACAGTAATTCACGGCGGAGTCCTTTGGTCCCAGGCATCATCACAGTTATTGACACCGAAACCGTTGGAGAGGGC
CAGGTGCTTGTGAGTGAGGATTCTGACAGTGATGGCATTGTGGCCCACTTCCCTGCCCATGAGAAGCCAGTGTGCTGCATGGCTTTTAAT
ACAAGTGGAATGCTTCTAGTCACAACAGACACCCTTGGCCATGACTTTCATGTCTTCCAAATTCTGACTCATCCTTGGTCCTCATCACAA
TGTGCTGTCCACCATCTGTATACTCTTCACAGGGGAGAAACTGAAGCCAAAGTACAGGACATCTGCTTCAGCCATGACTGTCGCTGGGTT
GTGGTCAGTACTCTCCGGGGTACTTCCCACGTTTTCCCCATCAACCCTTATGGTGGCCAGCCTTGTGTTCGTACACATATGTCACCACGA
GTAGTGAATCGCATGAGCCGTTTCCAGAAAAGTGCTGGACTGGAAGAGATTGAACAAGAACTGACGTCTAAGCAAGGAGGTCGCTGTAGC
CCTGTTCCAGGTCTATCAAGCAGCCCTTCTGGGTCACCCTTGCATGGGAAACTGAACAGCCAAGACTCCTATAACAATTTTACCAACAAC
AACCCTGGCAACCCTCGGCTCTCTCCTCTTCCCAGCTTGATGGTAGTGATGCCTCTTGCACAAATCAAGCAGCCAATGACATTGGGGACC
ATCACCAAACGAACCGGCAAAGTTAAACCTCCTCCACAAATTTCACCCAGCAAATCGATGGGCGGAGAATTTTGTGTGGCTGCTATCTTC
GGAACATCCAGGTCATGGTTTGCAAATAATGCAGGTCTGAAAAGAGAAAAAGATCAGTCCAAACAAGTTGTAGTTGAGTCCCTGTACATT
ATCAGTTGCTATGGCACCTTAGTGGAACACATGATGGAGCCGCGACCCCTCAGCACTGCACCCAAGATTAGTGACGACACACCACTGGAA
ATGATGACATCGCCTCGAGCCAGCTGGACTCTGGTTAGAACCCCTCAATGGAATGAATTGCAGCCACCGTTTAATGCAAACCACCCTCTG
CTCCTCGCTGCAGATGCAGTACAGTATTATCAGTTCCTGCTTGCTGGCCGCATCAAGACAGAAGACAGCTTGAACCATTCCCCTGGCCAG
AGTGGATTCCTCAGCTATGGCTCCAGCTTCAGCACCTCACCCACTGGACAGAGCCCATACACCTACCAGATGCACGGCACAACAGGGTTC
TATCAAGGAGGAAATGGACTGGGCAACGCAGCCGGTTTCGGGAGTGTGCACCAGGACTATCCTTCCTACCCCGGCTTCCCCCAGAGCCAG
TACCCCCAGTATTACGGCTCATCCTACAACCCTCCCTACGTCCCGGCCAGCAGCATCTGCCCTTCGCCCCTCTCCACGTCCACCTACGTC
CTCCAGGAGGCATCTCACAACGTCCCCAACCAGAGTTCCGAGTCACTTGCTGGTGAATACAACACACACAATGGACCTTCCACACCAGCG
AAAGAGGGAGACACAGACAGGCCGCACCGGGCCTCCGACGGGAAGCTCCGAGGCCGGTCTAAGAGGAGCAGTGACCCGTCCCCGGCAGGG
GACAATGAGATTGAGCGTGTGTTCGTGTGGGACTTGGATGAGACAATAATTATTTTTCACTCCTTACTCACGGGGACATTTGCATCCAGA
TACGGGAAGGACACCACGACGTCCGTGCGCATTGGCCTTATGATGGAAGAGATGATCTTCAACCTTGCAGATACACATCTGTTCTTCAAT
GACCTGGAGGATTGTGACCAGATCCACGTTGATGACGTCTCATCAGATGACAATGGCCAAGATTTAAGCACATACAACTTCTCCGCTGAC
GGCTTCCACAGTTCGGCCCCAGGAGCCAACCTGTGCCTGGGCTCTGGCGTGCACGGCGGCGTGGACTGGATGAGGAAGCTGGCCTTCCGC
TACCGGCGGGTGAAGGAGATGTACAATACCTACAAGAACAACGTTGGTGGGTTGATAGGCACTCCCAAAAGGGAGACCTGGCTACAGCTC
CGAGCTGAGCTGGAAGCTCTCACAGACCTCTGGCTGACCCACTCCCTGAAGGCACTAAACCTCATCAACTCCCGGCCCAACTGTGTCAAT
GTGCTGGTCACCACCACTCAACTAATTCCTGCCCTGGCCAAAGTCCTGCTATATGGCCTGGGGTCTGTGTTTCCTATTGAGAACATCTAC
AGTGCAACCAAGACAGGGAAGGAGAGCTGCTTCGAGAGGATAATGCAGAGATTCGGCAGAAAAGCTGTCTACGTGGTGATCGGTGATGGT
GTGGAAGAGGAGCAAGGAGCGAAAAAGCACAACATGCCTTTCTGGCGGATATCCTGCCACGCAGACCTGGAGGCACTGAGGCACGCCCTG
GAGCTGGAGTATTTATAGCAGGATCAGCAGCATCTCCACCTGCCATCTCACCCTCAGACCCCCTCGCCTTCCCCACCTCCCCACCGAGAA
CTCCAGAGACCCAGATGTTGGACACCAGGAAGGGGCCCCACAGCCGAGACGACGTGTCCAGTGACCATCTCAGAAGCCGTCCATCAGTCC
AAATGGGGGTTCTGAGAAGGAAAGTACCCAACATTGGCTTCGGAGTATTTGACTTTGGGGAAAAGGGCTGGCTCGGAGTCTAGACTCTTC
TGTAAGACTCACAGAACAAAAGCAAGGAATTGCTGATTTGGGGGGTGCCTGGTGATGAGGAGGGGATGGGTTTGTCTTGTCTTCTTTTTA
ATTTATGGACTAGTCTCATTACTCCGGAATTATGCTCTTGTACCTGTGTGGCTGGGTTTCTTAGTCGTTGGTTTGGTTTGGTTTTTTGAA
CTGGTATGTGGGGTGGTTCACAGTTCTAATGTAAGCACTCTATTCTCCAAGTTGTGCTTTGTGGGGACAATCATTCTTTGAACATTAGAG
AGGAAGGCAGTTCAAGCTGTTGAAAAGACTATTGCTTATTTTTGTTTTTAAAGACCTACTTGACGTCATGTGGACAGTGCACGTGCCTTA
CGCTACATCTTGTTTTCTAGGAAGAGGGGGATGCTGGGAAGGAATGGGTGCTTTGTGATGGATAAAAGGCATTAAATAAAACCACGTTTA

>In-frame_ENST00000408905_ENST00000327619_TCGA-UL-AAZ6-01A_BCAS3_chr17_59118253_+_EYA2_chr20_45644820_length(amino acids)=1115AA_start in transcript=0_stop in transcript=3347
MNEAMATDSPRRPSRCTGGVVVRPQAVTEQSYMESVVTFLQDVVPQAYSGTPLTEEKEKIVWVRFENADLNDTSRNLEFHEIHSTGNEPP
LLIMIGYSDGMQVWSIPISGEAQELFSVRHGPIRAARILPAPQFGAQKCDNFAEKRPLLGVCKSIGSSGTSPPYCCVDLYSLRTGEMVKS
IQFKTPIYDLHCNKRILVVVLQEKIAAFDSCTFTKKFFVTSCYPCPGPNMNPIALGSRWLAYAENKLIRCHQSRGGACGDNIQSYTATVI
SAAKTLKSGLTMVGKVVTQLTGTLPSGVTEDDVAIHSNSRRSPLVPGIITVIDTETVGEGQVLVSEDSDSDGIVAHFPAHEKPVCCMAFN
TSGMLLVTTDTLGHDFHVFQILTHPWSSSQCAVHHLYTLHRGETEAKVQDICFSHDCRWVVVSTLRGTSHVFPINPYGGQPCVRTHMSPR
VVNRMSRFQKSAGLEEIEQELTSKQGGRCSPVPGLSSSPSGSPLHGKLNSQDSYNNFTNNNPGNPRLSPLPSLMVVMPLAQIKQPMTLGT
ITKRTGKVKPPPQISPSKSMGGEFCVAAIFGTSRSWFANNAGLKREKDQSKQVVVESLYIISCYGTLVEHMMEPRPLSTAPKISDDTPLE
MMTSPRASWTLVRTPQWNELQPPFNANHPLLLAADAVQYYQFLLAGRIKTEDSLNHSPGQSGFLSYGSSFSTSPTGQSPYTYQMHGTTGF
YQGGNGLGNAAGFGSVHQDYPSYPGFPQSQYPQYYGSSYNPPYVPASSICPSPLSTSTYVLQEASHNVPNQSSESLAGEYNTHNGPSTPA
KEGDTDRPHRASDGKLRGRSKRSSDPSPAGDNEIERVFVWDLDETIIIFHSLLTGTFASRYGKDTTTSVRIGLMMEEMIFNLADTHLFFN
DLEDCDQIHVDDVSSDDNGQDLSTYNFSADGFHSSAPGANLCLGSGVHGGVDWMRKLAFRYRRVKEMYNTYKNNVGGLIGTPKRETWLQL
RAELEALTDLWLTHSLKALNLINSRPNCVNVLVTTTQLIPALAKVLLYGLGSVFPIENIYSATKTGKESCFERIMQRFGRKAVYVVIGDG

--------------------------------------------------------------
>In-frame_ENST00000588462_ENST00000357410_TCGA-UL-AAZ6-01A_BCAS3_chr17_59118253_+_EYA2_chr20_45644820_length(transcript)=3867nt_BP=2074nt
ATGAATGAAGCTATGGCTACAGATTCCCCAAGAAGACCCAGTCGTTGTACTGGTGGAGTTGTGGTTCGCCCCCAGGCTGTCACAGAGCAG
TCCTACATGGAAAGTGTTGTGACTTTTCTGCAGGATGTTGTGCCACAGGCTTACAGTGGAACACCTCTAACAGAAGAAAAGGAGAAAATA
GTCTGGGTCAGATTTGAAAATGCAGATTTAAATGATACATCAAGAAATCTGGAATTTCATGAAATACATAGTACTGGGAATGAACCGCCT
TTGTTGATTATGATTGGCTACAGTGATGGAATGCAGGTCTGGAGCATCCCTATCAGTGGTGAAGCACAAGAGCTCTTCTCTGTTCGACAT
GGCCCAATTCGAGCGGCTAGAATCTTGCCTGCTCCACAGTTTGGTGCTCAAAAATGTGATAACTTTGCTGAAAAAAGACCCCTCCTTGGT
GTTTGTAAGAGCATTGGATCTTCTGGCACAAGCCCACCGTACTGTTGTGTGGATCTGTATTCACTTCGTACTGGGGAGATGGTCAAGTCC
ATTCAATTTAAGACACCTATTTATGATCTCCATTGCAATAAACGGATCCTTGTCGTAGTCTTGCAGGAGAAAATTGCTGCCTTTGATAGC
TGTACTTTCACGAAGAAATTCTTTGTTACAAGCTGCTATCCATGTCCAGGGCCAAACATGAATCCTATTGCTCTTGGGAGCCGCTGGCTT
GCTTATGCAGAAAACAAGTTGATTCGATGTCATCAGTCCCGTGGTGGAGCCTGTGGAGACAACATTCAGTCTTATACTGCCACAGTCATT
AGTGCTGCTAAAACATTGAAAAGTGGCCTGACAATGGTAGGGAAAGTGGTGACTCAGCTGACAGGCACACTGCCTTCAGGTGTGACAGAA
GATGATGTTGCCATCCACAGTAATTCACGGCGGAGTCCTTTGGTCCCAGGCATCATCACAGTTATTGACACCGAAACCGTTGGAGAGGGC
CAGGTGCTTGTGAGTGAGGATTCTGACAGTGATGGCATTGTGGCCCACTTCCCTGCCCATGAGAAGCCAGTGTGCTGCATGGCTTTTAAT
ACAAGTGGAATGCTTCTAGTCACAACAGACACCCTTGGCCATGACTTTCATGTCTTCCAAATTCTGACTCATCCTTGGTCCTCATCACAA
TGTGCTGTCCACCATCTGTATACTCTTCACAGGGGAGAAACTGAAGCCAAAGTACAGGACATCTGCTTCAGCCATGACTGTCGCTGGGTT
GTGGTCAGTACTCTCCGGGGTACTTCCCACGTTTTCCCCATCAACCCTTATGGTGGCCAGCCTTGTGTTCGTACACATATGTCACCACGA
GTAGTGAATCGCATGAGCCGTTTCCAGAAAAGTGCTGGACTGGAAGAGATTGAACAAGAACTGACGTCTAAGCAAGGAGGTCGCTGTAGC
CCTGTTCCAGGTCTATCAAGCAGCCCTTCTGGGTCACCCTTGCATGGGAAACTGAACAGCCAAGACTCCTATAACAATTTTACCAACAAC
AACCCTGGCAACCCTCGGCTCTCTCCTCTTCCCAGCTTGATGGTAGTGATGCCTCTTGCACAAATCAAGCAGCCAATGACATTGGGGACC
ATCACCAAACGAACCGGGCCTTATCTCTTTGGAGCGGGGTGTTTTTCCATAAAAGCCCCATGCAAAGTTAAACCTCCTCCACAAATTTCA
CCCAGCAAATCGATGGGCGGAGAATTTTGTGTGGCTGCTATCTTCGGAACATCCAGGTCATGGTTTGCAAATAATGCAGGTCTGAAAAGA
GAAAAAGATCAGTCCAAACAAGTTGTAGTTGAGTCCCTGTACATTATCAGTTGCTATGGCACCTTAGTGGAACACATGATGGAGCCGCGA
CCCCTCAGCACTGCACCCAAGATTAGTGACGACACACCACTGGAAATGATGACATCGCCTCGAGCCAGCTGGACTCTGGTTAGAACCCCT
CAATGGAATGAATTGCAGCCACCGTTTAATGCAAACCACCCTCTGCTCCTCGCTGCAGATGCAGTACAGTATTATCAGTTCCTGCTTGCT
GGCCGCATCAAGACAGAAGACAGCTTGAACCATTCCCCTGGCCAGAGTGGATTCCTCAGCTATGGCTCCAGCTTCAGCACCTCACCCACT
GGACAGAGCCCATACACCTACCAGATGCACGGCACAACAGGGTTCTATCAAGGAGGAAATGGACTGGGCAACGCAGCCGGTTTCGGGAGT
GTGCACCAGGACTATCCTTCCTACCCCGGCTTCCCCCAGAGCCAGTACCCCCAGTATTACGGCTCATCCTACAACCCTCCCTACGTCCCG
GCCAGCAGCATCTGCCCTTCGCCCCTCTCCACGTCCACCTACGTCCTCCAGGAGGCATCTCACAACGTCCCCAACCAGAGTTCCGAGTCA
CTTGCTGGTGAATACAACACACACAATGGACCTTCCACACCAGCGAAAGAGGGAGACACAGACAGGCCGCACCGGGCCTCCGACGGGAAG
CTCCGAGGCCGGTCTAAGAGGAGCAGTGACCCGTCCCCGGCAGGGGACAATGAGATTGAGCGTGTGTTCGTGTGGGACTTGGATGAGACA
ATAATTATTTTTCACTCCTTACTCACGGGGACATTTGCATCCAGATACGGGAAGGACACCACGACGTCCGTGCGCATTGGCCTTATGATG
GAAGAGATGATCTTCAACCTTGCAGATACACATCTGTTCTTCAATGACCTGGAGGATTGTGACCAGATCCACGTTGATGACGTCTCATCA
GATGACAATGGCCAAGATTTAAGCACATACAACTTCTCCGCTGACGGCTTCCACAGTTCGGCCCCAGGAGCCAACCTGTGCCTGGGCTCT
GGCGTGCACGGCGGCGTGGACTGGATGAGGAAGCTGGCCTTCCGCTACCGGCGGGTGAAGGAGATGTACAATACCTACAAGAACAACGTT
GGTGGGAAGGAGAGCTGCTTCGAGAGGATAATGCAGAGATTCGGCAGAAAAGCTGTCTACGTGGTGATCGGTGATGGTGTGGAAGAGGAG
CAAGGAGCGAAAAAGCACAACATGCCTTTCTGGCGGATATCCTGCCACGCAGACCTGGAGGCACTGAGGCACGCCCTGGAGCTGGAGTAT
TTATAGCAGGATCAGCAGCATCTCCACCTGCCATCTCACCCTCAGACCCCCTCGCCTTCCCCACCTCCCCACCGAGAACTCCAGAGACCC
AGATGTTGGACACCAGGAAGGGGCCCCACAGCCGAGACGACGTGTCCAGTGACCATCTCAGAAGCCGTCCATCAGTCCAAATGGGGGTTC
TGAGAAGGAAAGTACCCAACATTGGCTTCGGAGTATTTGACTTTGGGGAAAAGGGCTGGCTCGGAGTCTAGACTCTTCTGTAAGACTCAC
AGAACAAAAGCAAGGAATTGCTGATTTGGGGGGTGCCTGGTGATGAGGAGGGGATGGGTTTGTCTTGTCTTCTTTTTAATTTATGGACTA
GTCTCATTACTCCGGAATTATGCTCTTGTACCTGTGTGGCTGGGTTTCTTAGTCGTTGGTTTGGTTTGGTTTTTTGAACTGGTATGTGGG
GTGGTTCACAGTTCTAATGTAAGCACTCTATTCTCCAAGTTGTGCTTTGTGGGGACAATCATTCTTTGAACATTAGAGAGGAAGGCAGTT
CAAGCTGTTGAAAAGACTATTGCTTATTTTTGTTTTTAAAGACCTACTTGACGTCATGTGGACAGTGCACGTGCCTTACGCTACATCTTG

>In-frame_ENST00000588462_ENST00000357410_TCGA-UL-AAZ6-01A_BCAS3_chr17_59118253_+_EYA2_chr20_45644820_length(amino acids)=1051AA_start in transcript=0_stop in transcript=3155
MNEAMATDSPRRPSRCTGGVVVRPQAVTEQSYMESVVTFLQDVVPQAYSGTPLTEEKEKIVWVRFENADLNDTSRNLEFHEIHSTGNEPP
LLIMIGYSDGMQVWSIPISGEAQELFSVRHGPIRAARILPAPQFGAQKCDNFAEKRPLLGVCKSIGSSGTSPPYCCVDLYSLRTGEMVKS
IQFKTPIYDLHCNKRILVVVLQEKIAAFDSCTFTKKFFVTSCYPCPGPNMNPIALGSRWLAYAENKLIRCHQSRGGACGDNIQSYTATVI
SAAKTLKSGLTMVGKVVTQLTGTLPSGVTEDDVAIHSNSRRSPLVPGIITVIDTETVGEGQVLVSEDSDSDGIVAHFPAHEKPVCCMAFN
TSGMLLVTTDTLGHDFHVFQILTHPWSSSQCAVHHLYTLHRGETEAKVQDICFSHDCRWVVVSTLRGTSHVFPINPYGGQPCVRTHMSPR
VVNRMSRFQKSAGLEEIEQELTSKQGGRCSPVPGLSSSPSGSPLHGKLNSQDSYNNFTNNNPGNPRLSPLPSLMVVMPLAQIKQPMTLGT
ITKRTGPYLFGAGCFSIKAPCKVKPPPQISPSKSMGGEFCVAAIFGTSRSWFANNAGLKREKDQSKQVVVESLYIISCYGTLVEHMMEPR
PLSTAPKISDDTPLEMMTSPRASWTLVRTPQWNELQPPFNANHPLLLAADAVQYYQFLLAGRIKTEDSLNHSPGQSGFLSYGSSFSTSPT
GQSPYTYQMHGTTGFYQGGNGLGNAAGFGSVHQDYPSYPGFPQSQYPQYYGSSYNPPYVPASSICPSPLSTSTYVLQEASHNVPNQSSES
LAGEYNTHNGPSTPAKEGDTDRPHRASDGKLRGRSKRSSDPSPAGDNEIERVFVWDLDETIIIFHSLLTGTFASRYGKDTTTSVRIGLMM
EEMIFNLADTHLFFNDLEDCDQIHVDDVSSDDNGQDLSTYNFSADGFHSSAPGANLCLGSGVHGGVDWMRKLAFRYRRVKEMYNTYKNNV

--------------------------------------------------------------
>In-frame_ENST00000588462_ENST00000327619_TCGA-UL-AAZ6-01A_BCAS3_chr17_59118253_+_EYA2_chr20_45644820_length(transcript)=4104nt_BP=2074nt
ATGAATGAAGCTATGGCTACAGATTCCCCAAGAAGACCCAGTCGTTGTACTGGTGGAGTTGTGGTTCGCCCCCAGGCTGTCACAGAGCAG
TCCTACATGGAAAGTGTTGTGACTTTTCTGCAGGATGTTGTGCCACAGGCTTACAGTGGAACACCTCTAACAGAAGAAAAGGAGAAAATA
GTCTGGGTCAGATTTGAAAATGCAGATTTAAATGATACATCAAGAAATCTGGAATTTCATGAAATACATAGTACTGGGAATGAACCGCCT
TTGTTGATTATGATTGGCTACAGTGATGGAATGCAGGTCTGGAGCATCCCTATCAGTGGTGAAGCACAAGAGCTCTTCTCTGTTCGACAT
GGCCCAATTCGAGCGGCTAGAATCTTGCCTGCTCCACAGTTTGGTGCTCAAAAATGTGATAACTTTGCTGAAAAAAGACCCCTCCTTGGT
GTTTGTAAGAGCATTGGATCTTCTGGCACAAGCCCACCGTACTGTTGTGTGGATCTGTATTCACTTCGTACTGGGGAGATGGTCAAGTCC
ATTCAATTTAAGACACCTATTTATGATCTCCATTGCAATAAACGGATCCTTGTCGTAGTCTTGCAGGAGAAAATTGCTGCCTTTGATAGC
TGTACTTTCACGAAGAAATTCTTTGTTACAAGCTGCTATCCATGTCCAGGGCCAAACATGAATCCTATTGCTCTTGGGAGCCGCTGGCTT
GCTTATGCAGAAAACAAGTTGATTCGATGTCATCAGTCCCGTGGTGGAGCCTGTGGAGACAACATTCAGTCTTATACTGCCACAGTCATT
AGTGCTGCTAAAACATTGAAAAGTGGCCTGACAATGGTAGGGAAAGTGGTGACTCAGCTGACAGGCACACTGCCTTCAGGTGTGACAGAA
GATGATGTTGCCATCCACAGTAATTCACGGCGGAGTCCTTTGGTCCCAGGCATCATCACAGTTATTGACACCGAAACCGTTGGAGAGGGC
CAGGTGCTTGTGAGTGAGGATTCTGACAGTGATGGCATTGTGGCCCACTTCCCTGCCCATGAGAAGCCAGTGTGCTGCATGGCTTTTAAT
ACAAGTGGAATGCTTCTAGTCACAACAGACACCCTTGGCCATGACTTTCATGTCTTCCAAATTCTGACTCATCCTTGGTCCTCATCACAA
TGTGCTGTCCACCATCTGTATACTCTTCACAGGGGAGAAACTGAAGCCAAAGTACAGGACATCTGCTTCAGCCATGACTGTCGCTGGGTT
GTGGTCAGTACTCTCCGGGGTACTTCCCACGTTTTCCCCATCAACCCTTATGGTGGCCAGCCTTGTGTTCGTACACATATGTCACCACGA
GTAGTGAATCGCATGAGCCGTTTCCAGAAAAGTGCTGGACTGGAAGAGATTGAACAAGAACTGACGTCTAAGCAAGGAGGTCGCTGTAGC
CCTGTTCCAGGTCTATCAAGCAGCCCTTCTGGGTCACCCTTGCATGGGAAACTGAACAGCCAAGACTCCTATAACAATTTTACCAACAAC
AACCCTGGCAACCCTCGGCTCTCTCCTCTTCCCAGCTTGATGGTAGTGATGCCTCTTGCACAAATCAAGCAGCCAATGACATTGGGGACC
ATCACCAAACGAACCGGGCCTTATCTCTTTGGAGCGGGGTGTTTTTCCATAAAAGCCCCATGCAAAGTTAAACCTCCTCCACAAATTTCA
CCCAGCAAATCGATGGGCGGAGAATTTTGTGTGGCTGCTATCTTCGGAACATCCAGGTCATGGTTTGCAAATAATGCAGGTCTGAAAAGA
GAAAAAGATCAGTCCAAACAAGTTGTAGTTGAGTCCCTGTACATTATCAGTTGCTATGGCACCTTAGTGGAACACATGATGGAGCCGCGA
CCCCTCAGCACTGCACCCAAGATTAGTGACGACACACCACTGGAAATGATGACATCGCCTCGAGCCAGCTGGACTCTGGTTAGAACCCCT
CAATGGAATGAATTGCAGCCACCGTTTAATGCAAACCACCCTCTGCTCCTCGCTGCAGATGCAGTACAGTATTATCAGTTCCTGCTTGCT
GGCCGCATCAAGACAGAAGACAGCTTGAACCATTCCCCTGGCCAGAGTGGATTCCTCAGCTATGGCTCCAGCTTCAGCACCTCACCCACT
GGACAGAGCCCATACACCTACCAGATGCACGGCACAACAGGGTTCTATCAAGGAGGAAATGGACTGGGCAACGCAGCCGGTTTCGGGAGT
GTGCACCAGGACTATCCTTCCTACCCCGGCTTCCCCCAGAGCCAGTACCCCCAGTATTACGGCTCATCCTACAACCCTCCCTACGTCCCG
GCCAGCAGCATCTGCCCTTCGCCCCTCTCCACGTCCACCTACGTCCTCCAGGAGGCATCTCACAACGTCCCCAACCAGAGTTCCGAGTCA
CTTGCTGGTGAATACAACACACACAATGGACCTTCCACACCAGCGAAAGAGGGAGACACAGACAGGCCGCACCGGGCCTCCGACGGGAAG
CTCCGAGGCCGGTCTAAGAGGAGCAGTGACCCGTCCCCGGCAGGGGACAATGAGATTGAGCGTGTGTTCGTGTGGGACTTGGATGAGACA
ATAATTATTTTTCACTCCTTACTCACGGGGACATTTGCATCCAGATACGGGAAGGACACCACGACGTCCGTGCGCATTGGCCTTATGATG
GAAGAGATGATCTTCAACCTTGCAGATACACATCTGTTCTTCAATGACCTGGAGGATTGTGACCAGATCCACGTTGATGACGTCTCATCA
GATGACAATGGCCAAGATTTAAGCACATACAACTTCTCCGCTGACGGCTTCCACAGTTCGGCCCCAGGAGCCAACCTGTGCCTGGGCTCT
GGCGTGCACGGCGGCGTGGACTGGATGAGGAAGCTGGCCTTCCGCTACCGGCGGGTGAAGGAGATGTACAATACCTACAAGAACAACGTT
GGTGGGTTGATAGGCACTCCCAAAAGGGAGACCTGGCTACAGCTCCGAGCTGAGCTGGAAGCTCTCACAGACCTCTGGCTGACCCACTCC
CTGAAGGCACTAAACCTCATCAACTCCCGGCCCAACTGTGTCAATGTGCTGGTCACCACCACTCAACTAATTCCTGCCCTGGCCAAAGTC
CTGCTATATGGCCTGGGGTCTGTGTTTCCTATTGAGAACATCTACAGTGCAACCAAGACAGGGAAGGAGAGCTGCTTCGAGAGGATAATG
CAGAGATTCGGCAGAAAAGCTGTCTACGTGGTGATCGGTGATGGTGTGGAAGAGGAGCAAGGAGCGAAAAAGCACAACATGCCTTTCTGG
CGGATATCCTGCCACGCAGACCTGGAGGCACTGAGGCACGCCCTGGAGCTGGAGTATTTATAGCAGGATCAGCAGCATCTCCACCTGCCA
TCTCACCCTCAGACCCCCTCGCCTTCCCCACCTCCCCACCGAGAACTCCAGAGACCCAGATGTTGGACACCAGGAAGGGGCCCCACAGCC
GAGACGACGTGTCCAGTGACCATCTCAGAAGCCGTCCATCAGTCCAAATGGGGGTTCTGAGAAGGAAAGTACCCAACATTGGCTTCGGAG
TATTTGACTTTGGGGAAAAGGGCTGGCTCGGAGTCTAGACTCTTCTGTAAGACTCACAGAACAAAAGCAAGGAATTGCTGATTTGGGGGG
TGCCTGGTGATGAGGAGGGGATGGGTTTGTCTTGTCTTCTTTTTAATTTATGGACTAGTCTCATTACTCCGGAATTATGCTCTTGTACCT
GTGTGGCTGGGTTTCTTAGTCGTTGGTTTGGTTTGGTTTTTTGAACTGGTATGTGGGGTGGTTCACAGTTCTAATGTAAGCACTCTATTC
TCCAAGTTGTGCTTTGTGGGGACAATCATTCTTTGAACATTAGAGAGGAAGGCAGTTCAAGCTGTTGAAAAGACTATTGCTTATTTTTGT
TTTTAAAGACCTACTTGACGTCATGTGGACAGTGCACGTGCCTTACGCTACATCTTGTTTTCTAGGAAGAGGGGGATGCTGGGAAGGAAT

>In-frame_ENST00000588462_ENST00000327619_TCGA-UL-AAZ6-01A_BCAS3_chr17_59118253_+_EYA2_chr20_45644820_length(amino acids)=1130AA_start in transcript=0_stop in transcript=3392
MNEAMATDSPRRPSRCTGGVVVRPQAVTEQSYMESVVTFLQDVVPQAYSGTPLTEEKEKIVWVRFENADLNDTSRNLEFHEIHSTGNEPP
LLIMIGYSDGMQVWSIPISGEAQELFSVRHGPIRAARILPAPQFGAQKCDNFAEKRPLLGVCKSIGSSGTSPPYCCVDLYSLRTGEMVKS
IQFKTPIYDLHCNKRILVVVLQEKIAAFDSCTFTKKFFVTSCYPCPGPNMNPIALGSRWLAYAENKLIRCHQSRGGACGDNIQSYTATVI
SAAKTLKSGLTMVGKVVTQLTGTLPSGVTEDDVAIHSNSRRSPLVPGIITVIDTETVGEGQVLVSEDSDSDGIVAHFPAHEKPVCCMAFN
TSGMLLVTTDTLGHDFHVFQILTHPWSSSQCAVHHLYTLHRGETEAKVQDICFSHDCRWVVVSTLRGTSHVFPINPYGGQPCVRTHMSPR
VVNRMSRFQKSAGLEEIEQELTSKQGGRCSPVPGLSSSPSGSPLHGKLNSQDSYNNFTNNNPGNPRLSPLPSLMVVMPLAQIKQPMTLGT
ITKRTGPYLFGAGCFSIKAPCKVKPPPQISPSKSMGGEFCVAAIFGTSRSWFANNAGLKREKDQSKQVVVESLYIISCYGTLVEHMMEPR
PLSTAPKISDDTPLEMMTSPRASWTLVRTPQWNELQPPFNANHPLLLAADAVQYYQFLLAGRIKTEDSLNHSPGQSGFLSYGSSFSTSPT
GQSPYTYQMHGTTGFYQGGNGLGNAAGFGSVHQDYPSYPGFPQSQYPQYYGSSYNPPYVPASSICPSPLSTSTYVLQEASHNVPNQSSES
LAGEYNTHNGPSTPAKEGDTDRPHRASDGKLRGRSKRSSDPSPAGDNEIERVFVWDLDETIIIFHSLLTGTFASRYGKDTTTSVRIGLMM
EEMIFNLADTHLFFNDLEDCDQIHVDDVSSDDNGQDLSTYNFSADGFHSSAPGANLCLGSGVHGGVDWMRKLAFRYRRVKEMYNTYKNNV
GGLIGTPKRETWLQLRAELEALTDLWLTHSLKALNLINSRPNCVNVLVTTTQLIPALAKVLLYGLGSVFPIENIYSATKTGKESCFERIM

--------------------------------------------------------------
>In-frame_ENST00000585744_ENST00000357410_TCGA-UL-AAZ6-01A_BCAS3_chr17_59118253_+_EYA2_chr20_45644820_length(transcript)=3283nt_BP=1490nt
GCCGCCTTGCAGTTTGATCTCAGACTGCTGTGCTGGCAATCAGCGAGACTCCGTGGGCGTAGGACCCTCCGAGCCAGGCTGCTATCCATG
TCCAGGGCCAAACATGAATCCTATTGCTCTTGGGAGCCGCTGGCTTGCTTATGCAGAAAACAAGTTGATTCGATGTCATCAGTCCCGTGG
TGGAGCCTGTGGAGACAACATTCAGTCTTATACTGCCACAGTCATTAGTGCTGCTAAAACATTGAAAAGTGGCCTGACAATGGTAGGGAA
AGTGGTGACTCAGCTGACAGGCACACTGCCTTCAGGTGTGACAGAAGATGATGTTGCCATCCACAGTAATTCACGGCGGAGTCCTTTGGT
CCCAGGCATCATCACAGTTATTGACACCGAAACCGTTGGAGAGGGCCAGGTGCTTGTGAGTGAGGATTCTGACAGTGATGGCATTGTGGC
CCACTTCCCTGCCCATGAGAAGCCAGTGTGCTGCATGGCTTTTAATACAAGTGGAATGCTTCTAGTCACAACAGACACCCTTGGCCATGA
CTTTCATGTCTTCCAAATTCTGACTCATCCTTGGTCCTCATCACAATGTGCTGTCCACCATCTGTATACTCTTCACAGGGGAGAAACTGA
AGCCAAAGTACAGGACATCTGCTTCAGCCATGACTGTCGCTGGGTTGTGGTCAGTACTCTCCGGGGTACTTCCCACGTTTTCCCCATCAA
CCCTTATGGTGGCCAGCCTTGTGTTCGTACACATATGTCACCACGAGTAGTGAATCGCATGAGCCGTTTCCAGAAAAGTGCTGGACTGGA
AGAGATTGAACAAGAACTGACGTCTAAGCAAGGAGGTCGCTGTAGCCCTGTTCCAGGTCTATCAAGCAGCCCTTCTGGGTCACCCTTGCA
TGGGAAACTGAACAGCCAAGACTCCTATAACAATTTTACCAACAACAACCCTGGCAACCCTCGGCTCTCTCCTCTTCCCAGCTTGATGGT
AGTGATGCCTCTTGCACAAATCAAGCAGCCAATGACATTGGGGACCATCACCAAACGAACCGGGCCTTATCTCTTTGGAGCGGGGTGTTT
TTCCATAAAAGCCCCATGCAAAGTTAAACCTCCTCCACAAATTTCACCCAGCAAATCGATGGGCGGAGAATTTTGTGTGGCTGCTATCTT
CGGAACATCCAGGTCATGGTTTGCAAATAATGCAGGTCTGAAAAGAGAAAAAGATCAGTCCAAACAAGTTGTAGTTGAGTCCCTGTACAT
TATCAGTTGCTATGGCACCTTAGTGGAACACATGATGGAGCCGCGACCCCTCAGCACTGCACCCAAGATTAGTGACGACACACCACTGGA
AATGATGACATCGCCTCGAGCCAGCTGGACTCTGGTTAGAACCCCTCAATGGAATGAATTGCAGCCACCGTTTAATGCAAACCACCCTCT
GCTCCTCGCTGCAGATGCAGTACAGTATTATCAGTTCCTGCTTGCTGGCCGCATCAAGACAGAAGACAGCTTGAACCATTCCCCTGGCCA
GAGTGGATTCCTCAGCTATGGCTCCAGCTTCAGCACCTCACCCACTGGACAGAGCCCATACACCTACCAGATGCACGGCACAACAGGGTT
CTATCAAGGAGGAAATGGACTGGGCAACGCAGCCGGTTTCGGGAGTGTGCACCAGGACTATCCTTCCTACCCCGGCTTCCCCCAGAGCCA
GTACCCCCAGTATTACGGCTCATCCTACAACCCTCCCTACGTCCCGGCCAGCAGCATCTGCCCTTCGCCCCTCTCCACGTCCACCTACGT
CCTCCAGGAGGCATCTCACAACGTCCCCAACCAGAGTTCCGAGTCACTTGCTGGTGAATACAACACACACAATGGACCTTCCACACCAGC
GAAAGAGGGAGACACAGACAGGCCGCACCGGGCCTCCGACGGGAAGCTCCGAGGCCGGTCTAAGAGGAGCAGTGACCCGTCCCCGGCAGG
GGACAATGAGATTGAGCGTGTGTTCGTGTGGGACTTGGATGAGACAATAATTATTTTTCACTCCTTACTCACGGGGACATTTGCATCCAG
ATACGGGAAGGACACCACGACGTCCGTGCGCATTGGCCTTATGATGGAAGAGATGATCTTCAACCTTGCAGATACACATCTGTTCTTCAA
TGACCTGGAGGATTGTGACCAGATCCACGTTGATGACGTCTCATCAGATGACAATGGCCAAGATTTAAGCACATACAACTTCTCCGCTGA
CGGCTTCCACAGTTCGGCCCCAGGAGCCAACCTGTGCCTGGGCTCTGGCGTGCACGGCGGCGTGGACTGGATGAGGAAGCTGGCCTTCCG
CTACCGGCGGGTGAAGGAGATGTACAATACCTACAAGAACAACGTTGGTGGGAAGGAGAGCTGCTTCGAGAGGATAATGCAGAGATTCGG
CAGAAAAGCTGTCTACGTGGTGATCGGTGATGGTGTGGAAGAGGAGCAAGGAGCGAAAAAGCACAACATGCCTTTCTGGCGGATATCCTG
CCACGCAGACCTGGAGGCACTGAGGCACGCCCTGGAGCTGGAGTATTTATAGCAGGATCAGCAGCATCTCCACCTGCCATCTCACCCTCA
GACCCCCTCGCCTTCCCCACCTCCCCACCGAGAACTCCAGAGACCCAGATGTTGGACACCAGGAAGGGGCCCCACAGCCGAGACGACGTG
TCCAGTGACCATCTCAGAAGCCGTCCATCAGTCCAAATGGGGGTTCTGAGAAGGAAAGTACCCAACATTGGCTTCGGAGTATTTGACTTT
GGGGAAAAGGGCTGGCTCGGAGTCTAGACTCTTCTGTAAGACTCACAGAACAAAAGCAAGGAATTGCTGATTTGGGGGGTGCCTGGTGAT
GAGGAGGGGATGGGTTTGTCTTGTCTTCTTTTTAATTTATGGACTAGTCTCATTACTCCGGAATTATGCTCTTGTACCTGTGTGGCTGGG
TTTCTTAGTCGTTGGTTTGGTTTGGTTTTTTGAACTGGTATGTGGGGTGGTTCACAGTTCTAATGTAAGCACTCTATTCTCCAAGTTGTG
CTTTGTGGGGACAATCATTCTTTGAACATTAGAGAGGAAGGCAGTTCAAGCTGTTGAAAAGACTATTGCTTATTTTTGTTTTTAAAGACC
TACTTGACGTCATGTGGACAGTGCACGTGCCTTACGCTACATCTTGTTTTCTAGGAAGAGGGGGATGCTGGGAAGGAATGGGTGCTTTGT

>In-frame_ENST00000585744_ENST00000357410_TCGA-UL-AAZ6-01A_BCAS3_chr17_59118253_+_EYA2_chr20_45644820_length(amino acids)=852AA_start in transcript=13_stop in transcript=2571
MISDCCAGNQRDSVGVGPSEPGCYPCPGPNMNPIALGSRWLAYAENKLIRCHQSRGGACGDNIQSYTATVISAAKTLKSGLTMVGKVVTQ
LTGTLPSGVTEDDVAIHSNSRRSPLVPGIITVIDTETVGEGQVLVSEDSDSDGIVAHFPAHEKPVCCMAFNTSGMLLVTTDTLGHDFHVF
QILTHPWSSSQCAVHHLYTLHRGETEAKVQDICFSHDCRWVVVSTLRGTSHVFPINPYGGQPCVRTHMSPRVVNRMSRFQKSAGLEEIEQ
ELTSKQGGRCSPVPGLSSSPSGSPLHGKLNSQDSYNNFTNNNPGNPRLSPLPSLMVVMPLAQIKQPMTLGTITKRTGPYLFGAGCFSIKA
PCKVKPPPQISPSKSMGGEFCVAAIFGTSRSWFANNAGLKREKDQSKQVVVESLYIISCYGTLVEHMMEPRPLSTAPKISDDTPLEMMTS
PRASWTLVRTPQWNELQPPFNANHPLLLAADAVQYYQFLLAGRIKTEDSLNHSPGQSGFLSYGSSFSTSPTGQSPYTYQMHGTTGFYQGG
NGLGNAAGFGSVHQDYPSYPGFPQSQYPQYYGSSYNPPYVPASSICPSPLSTSTYVLQEASHNVPNQSSESLAGEYNTHNGPSTPAKEGD
TDRPHRASDGKLRGRSKRSSDPSPAGDNEIERVFVWDLDETIIIFHSLLTGTFASRYGKDTTTSVRIGLMMEEMIFNLADTHLFFNDLED
CDQIHVDDVSSDDNGQDLSTYNFSADGFHSSAPGANLCLGSGVHGGVDWMRKLAFRYRRVKEMYNTYKNNVGGKESCFERIMQRFGRKAV

--------------------------------------------------------------
>In-frame_ENST00000585744_ENST00000327619_TCGA-UL-AAZ6-01A_BCAS3_chr17_59118253_+_EYA2_chr20_45644820_length(transcript)=3520nt_BP=1490nt
GCCGCCTTGCAGTTTGATCTCAGACTGCTGTGCTGGCAATCAGCGAGACTCCGTGGGCGTAGGACCCTCCGAGCCAGGCTGCTATCCATG
TCCAGGGCCAAACATGAATCCTATTGCTCTTGGGAGCCGCTGGCTTGCTTATGCAGAAAACAAGTTGATTCGATGTCATCAGTCCCGTGG
TGGAGCCTGTGGAGACAACATTCAGTCTTATACTGCCACAGTCATTAGTGCTGCTAAAACATTGAAAAGTGGCCTGACAATGGTAGGGAA
AGTGGTGACTCAGCTGACAGGCACACTGCCTTCAGGTGTGACAGAAGATGATGTTGCCATCCACAGTAATTCACGGCGGAGTCCTTTGGT
CCCAGGCATCATCACAGTTATTGACACCGAAACCGTTGGAGAGGGCCAGGTGCTTGTGAGTGAGGATTCTGACAGTGATGGCATTGTGGC
CCACTTCCCTGCCCATGAGAAGCCAGTGTGCTGCATGGCTTTTAATACAAGTGGAATGCTTCTAGTCACAACAGACACCCTTGGCCATGA
CTTTCATGTCTTCCAAATTCTGACTCATCCTTGGTCCTCATCACAATGTGCTGTCCACCATCTGTATACTCTTCACAGGGGAGAAACTGA
AGCCAAAGTACAGGACATCTGCTTCAGCCATGACTGTCGCTGGGTTGTGGTCAGTACTCTCCGGGGTACTTCCCACGTTTTCCCCATCAA
CCCTTATGGTGGCCAGCCTTGTGTTCGTACACATATGTCACCACGAGTAGTGAATCGCATGAGCCGTTTCCAGAAAAGTGCTGGACTGGA
AGAGATTGAACAAGAACTGACGTCTAAGCAAGGAGGTCGCTGTAGCCCTGTTCCAGGTCTATCAAGCAGCCCTTCTGGGTCACCCTTGCA
TGGGAAACTGAACAGCCAAGACTCCTATAACAATTTTACCAACAACAACCCTGGCAACCCTCGGCTCTCTCCTCTTCCCAGCTTGATGGT
AGTGATGCCTCTTGCACAAATCAAGCAGCCAATGACATTGGGGACCATCACCAAACGAACCGGGCCTTATCTCTTTGGAGCGGGGTGTTT
TTCCATAAAAGCCCCATGCAAAGTTAAACCTCCTCCACAAATTTCACCCAGCAAATCGATGGGCGGAGAATTTTGTGTGGCTGCTATCTT
CGGAACATCCAGGTCATGGTTTGCAAATAATGCAGGTCTGAAAAGAGAAAAAGATCAGTCCAAACAAGTTGTAGTTGAGTCCCTGTACAT
TATCAGTTGCTATGGCACCTTAGTGGAACACATGATGGAGCCGCGACCCCTCAGCACTGCACCCAAGATTAGTGACGACACACCACTGGA
AATGATGACATCGCCTCGAGCCAGCTGGACTCTGGTTAGAACCCCTCAATGGAATGAATTGCAGCCACCGTTTAATGCAAACCACCCTCT
GCTCCTCGCTGCAGATGCAGTACAGTATTATCAGTTCCTGCTTGCTGGCCGCATCAAGACAGAAGACAGCTTGAACCATTCCCCTGGCCA
GAGTGGATTCCTCAGCTATGGCTCCAGCTTCAGCACCTCACCCACTGGACAGAGCCCATACACCTACCAGATGCACGGCACAACAGGGTT
CTATCAAGGAGGAAATGGACTGGGCAACGCAGCCGGTTTCGGGAGTGTGCACCAGGACTATCCTTCCTACCCCGGCTTCCCCCAGAGCCA
GTACCCCCAGTATTACGGCTCATCCTACAACCCTCCCTACGTCCCGGCCAGCAGCATCTGCCCTTCGCCCCTCTCCACGTCCACCTACGT
CCTCCAGGAGGCATCTCACAACGTCCCCAACCAGAGTTCCGAGTCACTTGCTGGTGAATACAACACACACAATGGACCTTCCACACCAGC
GAAAGAGGGAGACACAGACAGGCCGCACCGGGCCTCCGACGGGAAGCTCCGAGGCCGGTCTAAGAGGAGCAGTGACCCGTCCCCGGCAGG
GGACAATGAGATTGAGCGTGTGTTCGTGTGGGACTTGGATGAGACAATAATTATTTTTCACTCCTTACTCACGGGGACATTTGCATCCAG
ATACGGGAAGGACACCACGACGTCCGTGCGCATTGGCCTTATGATGGAAGAGATGATCTTCAACCTTGCAGATACACATCTGTTCTTCAA
TGACCTGGAGGATTGTGACCAGATCCACGTTGATGACGTCTCATCAGATGACAATGGCCAAGATTTAAGCACATACAACTTCTCCGCTGA
CGGCTTCCACAGTTCGGCCCCAGGAGCCAACCTGTGCCTGGGCTCTGGCGTGCACGGCGGCGTGGACTGGATGAGGAAGCTGGCCTTCCG
CTACCGGCGGGTGAAGGAGATGTACAATACCTACAAGAACAACGTTGGTGGGTTGATAGGCACTCCCAAAAGGGAGACCTGGCTACAGCT
CCGAGCTGAGCTGGAAGCTCTCACAGACCTCTGGCTGACCCACTCCCTGAAGGCACTAAACCTCATCAACTCCCGGCCCAACTGTGTCAA
TGTGCTGGTCACCACCACTCAACTAATTCCTGCCCTGGCCAAAGTCCTGCTATATGGCCTGGGGTCTGTGTTTCCTATTGAGAACATCTA
CAGTGCAACCAAGACAGGGAAGGAGAGCTGCTTCGAGAGGATAATGCAGAGATTCGGCAGAAAAGCTGTCTACGTGGTGATCGGTGATGG
TGTGGAAGAGGAGCAAGGAGCGAAAAAGCACAACATGCCTTTCTGGCGGATATCCTGCCACGCAGACCTGGAGGCACTGAGGCACGCCCT
GGAGCTGGAGTATTTATAGCAGGATCAGCAGCATCTCCACCTGCCATCTCACCCTCAGACCCCCTCGCCTTCCCCACCTCCCCACCGAGA
ACTCCAGAGACCCAGATGTTGGACACCAGGAAGGGGCCCCACAGCCGAGACGACGTGTCCAGTGACCATCTCAGAAGCCGTCCATCAGTC
CAAATGGGGGTTCTGAGAAGGAAAGTACCCAACATTGGCTTCGGAGTATTTGACTTTGGGGAAAAGGGCTGGCTCGGAGTCTAGACTCTT
CTGTAAGACTCACAGAACAAAAGCAAGGAATTGCTGATTTGGGGGGTGCCTGGTGATGAGGAGGGGATGGGTTTGTCTTGTCTTCTTTTT
AATTTATGGACTAGTCTCATTACTCCGGAATTATGCTCTTGTACCTGTGTGGCTGGGTTTCTTAGTCGTTGGTTTGGTTTGGTTTTTTGA
ACTGGTATGTGGGGTGGTTCACAGTTCTAATGTAAGCACTCTATTCTCCAAGTTGTGCTTTGTGGGGACAATCATTCTTTGAACATTAGA
GAGGAAGGCAGTTCAAGCTGTTGAAAAGACTATTGCTTATTTTTGTTTTTAAAGACCTACTTGACGTCATGTGGACAGTGCACGTGCCTT
ACGCTACATCTTGTTTTCTAGGAAGAGGGGGATGCTGGGAAGGAATGGGTGCTTTGTGATGGATAAAAGGCATTAAATAAAACCACGTTT

>In-frame_ENST00000585744_ENST00000327619_TCGA-UL-AAZ6-01A_BCAS3_chr17_59118253_+_EYA2_chr20_45644820_length(amino acids)=931AA_start in transcript=13_stop in transcript=2808
MISDCCAGNQRDSVGVGPSEPGCYPCPGPNMNPIALGSRWLAYAENKLIRCHQSRGGACGDNIQSYTATVISAAKTLKSGLTMVGKVVTQ
LTGTLPSGVTEDDVAIHSNSRRSPLVPGIITVIDTETVGEGQVLVSEDSDSDGIVAHFPAHEKPVCCMAFNTSGMLLVTTDTLGHDFHVF
QILTHPWSSSQCAVHHLYTLHRGETEAKVQDICFSHDCRWVVVSTLRGTSHVFPINPYGGQPCVRTHMSPRVVNRMSRFQKSAGLEEIEQ
ELTSKQGGRCSPVPGLSSSPSGSPLHGKLNSQDSYNNFTNNNPGNPRLSPLPSLMVVMPLAQIKQPMTLGTITKRTGPYLFGAGCFSIKA
PCKVKPPPQISPSKSMGGEFCVAAIFGTSRSWFANNAGLKREKDQSKQVVVESLYIISCYGTLVEHMMEPRPLSTAPKISDDTPLEMMTS
PRASWTLVRTPQWNELQPPFNANHPLLLAADAVQYYQFLLAGRIKTEDSLNHSPGQSGFLSYGSSFSTSPTGQSPYTYQMHGTTGFYQGG
NGLGNAAGFGSVHQDYPSYPGFPQSQYPQYYGSSYNPPYVPASSICPSPLSTSTYVLQEASHNVPNQSSESLAGEYNTHNGPSTPAKEGD
TDRPHRASDGKLRGRSKRSSDPSPAGDNEIERVFVWDLDETIIIFHSLLTGTFASRYGKDTTTSVRIGLMMEEMIFNLADTHLFFNDLED
CDQIHVDDVSSDDNGQDLSTYNFSADGFHSSAPGANLCLGSGVHGGVDWMRKLAFRYRRVKEMYNTYKNNVGGLIGTPKRETWLQLRAEL
EALTDLWLTHSLKALNLINSRPNCVNVLVTTTQLIPALAKVLLYGLGSVFPIENIYSATKTGKESCFERIMQRFGRKAVYVVIGDGVEEE

--------------------------------------------------------------
>In-frame_ENST00000588874_ENST00000357410_TCGA-UL-AAZ6-01A_BCAS3_chr17_59118253_+_EYA2_chr20_45644820_length(transcript)=3323nt_BP=1530nt
GCCGCCTTGCAGTTTGATCTCAGACTGCTGTGCTGGCAATCAGCGAGACTCCGTGGGCGTAGGACCCTCCGAGCCAGGTGTGGGATATAG
TCTCGTGGTGCGCCGTTTTTTAAGCCGGTCTGAAAAGCGCAATATTCGGGTGGGAGTGACCCGATTTTCCAGGCTGCTATCCATGTCCAG
GGCCAAACATGAATCCTATTGCTCTTGGGAGCCGCTGGCTTGCTTATGCAGAAAACAAGTTGATTCGATGTCATCAGTCCCGTGGTGGAG
CCTGTGGAGACAACATTCAGTCTTATACTGCCACAGTCATTAGTGCTGCTAAAACATTGAAAAGTGGCCTGACAATGGTAGGGAAAGTGG
TGACTCAGCTGACAGGCACACTGCCTTCAGGTGTGACAGAAGATGATGTTGCCATCCACAGTAATTCACGGCGGAGTCCTTTGGTCCCAG
GCATCATCACAGTTATTGACACCGAAACCGTTGGAGAGGGCCAGGTGCTTGTGAGTGAGGATTCTGACAGTGATGGCATTGTGGCCCACT
TCCCTGCCCATGAGAAGCCAGTGTGCTGCATGGCTTTTAATACAAGTGGAATGCTTCTAGTCACAACAGACACCCTTGGCCATGACTTTC
ATGTCTTCCAAATTCTGACTCATCCTTGGTCCTCATCACAATGTGCTGTCCACCATCTGTATACTCTTCACAGGGGAGAAACTGAAGCCA
AAGTACAGGACATCTGCTTCAGCCATGACTGTCGCTGGGTTGTGGTCAGTACTCTCCGGGGTACTTCCCACGTTTTCCCCATCAACCCTT
ATGGTGGCCAGCCTTGTGTTCGTACACATATGTCACCACGAGTAGTGAATCGCATGAGCCGTTTCCAGAAAAGTGCTGGACTGGAAGAGA
TTGAACAAGAACTGACGTCTAAGCAAGGAGGTCGCTGTAGCCCTGTTCCAGGTCTATCAAGCAGCCCTTCTGGGTCACCCTTGCATGGGA
AACTGAACAGCCAAGACTCCTATAACAATTTTACCAACAACAACCCTGGCAACCCTCGGCTCTCTCCTCTTCCCAGCTTGATGGTAGTGA
TGCCTCTTGCACAAATCAAGCAGCCAATGACATTGGGGACCATCACCAAACGAACCGGCAAAGTTAAACCTCCTCCACAAATTTCACCCA
GCAAATCGATGGGCGGAGAATTTTGTGTGGCTGCTATCTTCGGAACATCCAGGTCATGGTTTGCAAATAATGCAGGTCTGAAAAGAGAAA
AAGATCAGTCCAAACAAGTTGTAGTTGAGTCCCTGTACATTATCAGTTGCTATGGCACCTTAGTGGAACACATGATGGAGCCGCGACCCC
TCAGCACTGCACCCAAGATTAGTGACGACACACCACTGGAAATGATGACATCGCCTCGAGCCAGCTGGACTCTGGTTAGAACCCCTCAAT
GGAATGAATTGCAGCCACCGTTTAATGCAAACCACCCTCTGCTCCTCGCTGCAGATGCAGTACAGTATTATCAGTTCCTGCTTGCTGGCC
GCATCAAGACAGAAGACAGCTTGAACCATTCCCCTGGCCAGAGTGGATTCCTCAGCTATGGCTCCAGCTTCAGCACCTCACCCACTGGAC
AGAGCCCATACACCTACCAGATGCACGGCACAACAGGGTTCTATCAAGGAGGAAATGGACTGGGCAACGCAGCCGGTTTCGGGAGTGTGC
ACCAGGACTATCCTTCCTACCCCGGCTTCCCCCAGAGCCAGTACCCCCAGTATTACGGCTCATCCTACAACCCTCCCTACGTCCCGGCCA
GCAGCATCTGCCCTTCGCCCCTCTCCACGTCCACCTACGTCCTCCAGGAGGCATCTCACAACGTCCCCAACCAGAGTTCCGAGTCACTTG
CTGGTGAATACAACACACACAATGGACCTTCCACACCAGCGAAAGAGGGAGACACAGACAGGCCGCACCGGGCCTCCGACGGGAAGCTCC
GAGGCCGGTCTAAGAGGAGCAGTGACCCGTCCCCGGCAGGGGACAATGAGATTGAGCGTGTGTTCGTGTGGGACTTGGATGAGACAATAA
TTATTTTTCACTCCTTACTCACGGGGACATTTGCATCCAGATACGGGAAGGACACCACGACGTCCGTGCGCATTGGCCTTATGATGGAAG
AGATGATCTTCAACCTTGCAGATACACATCTGTTCTTCAATGACCTGGAGGATTGTGACCAGATCCACGTTGATGACGTCTCATCAGATG
ACAATGGCCAAGATTTAAGCACATACAACTTCTCCGCTGACGGCTTCCACAGTTCGGCCCCAGGAGCCAACCTGTGCCTGGGCTCTGGCG
TGCACGGCGGCGTGGACTGGATGAGGAAGCTGGCCTTCCGCTACCGGCGGGTGAAGGAGATGTACAATACCTACAAGAACAACGTTGGTG
GGAAGGAGAGCTGCTTCGAGAGGATAATGCAGAGATTCGGCAGAAAAGCTGTCTACGTGGTGATCGGTGATGGTGTGGAAGAGGAGCAAG
GAGCGAAAAAGCACAACATGCCTTTCTGGCGGATATCCTGCCACGCAGACCTGGAGGCACTGAGGCACGCCCTGGAGCTGGAGTATTTAT
AGCAGGATCAGCAGCATCTCCACCTGCCATCTCACCCTCAGACCCCCTCGCCTTCCCCACCTCCCCACCGAGAACTCCAGAGACCCAGAT
GTTGGACACCAGGAAGGGGCCCCACAGCCGAGACGACGTGTCCAGTGACCATCTCAGAAGCCGTCCATCAGTCCAAATGGGGGTTCTGAG
AAGGAAAGTACCCAACATTGGCTTCGGAGTATTTGACTTTGGGGAAAAGGGCTGGCTCGGAGTCTAGACTCTTCTGTAAGACTCACAGAA
CAAAAGCAAGGAATTGCTGATTTGGGGGGTGCCTGGTGATGAGGAGGGGATGGGTTTGTCTTGTCTTCTTTTTAATTTATGGACTAGTCT
CATTACTCCGGAATTATGCTCTTGTACCTGTGTGGCTGGGTTTCTTAGTCGTTGGTTTGGTTTGGTTTTTTGAACTGGTATGTGGGGTGG
TTCACAGTTCTAATGTAAGCACTCTATTCTCCAAGTTGTGCTTTGTGGGGACAATCATTCTTTGAACATTAGAGAGGAAGGCAGTTCAAG
CTGTTGAAAAGACTATTGCTTATTTTTGTTTTTAAAGACCTACTTGACGTCATGTGGACAGTGCACGTGCCTTACGCTACATCTTGTTTT

>In-frame_ENST00000588874_ENST00000357410_TCGA-UL-AAZ6-01A_BCAS3_chr17_59118253_+_EYA2_chr20_45644820_length(amino acids)=807AA_start in transcript=188_stop in transcript=2611
MNPIALGSRWLAYAENKLIRCHQSRGGACGDNIQSYTATVISAAKTLKSGLTMVGKVVTQLTGTLPSGVTEDDVAIHSNSRRSPLVPGII
TVIDTETVGEGQVLVSEDSDSDGIVAHFPAHEKPVCCMAFNTSGMLLVTTDTLGHDFHVFQILTHPWSSSQCAVHHLYTLHRGETEAKVQ
DICFSHDCRWVVVSTLRGTSHVFPINPYGGQPCVRTHMSPRVVNRMSRFQKSAGLEEIEQELTSKQGGRCSPVPGLSSSPSGSPLHGKLN
SQDSYNNFTNNNPGNPRLSPLPSLMVVMPLAQIKQPMTLGTITKRTGKVKPPPQISPSKSMGGEFCVAAIFGTSRSWFANNAGLKREKDQ
SKQVVVESLYIISCYGTLVEHMMEPRPLSTAPKISDDTPLEMMTSPRASWTLVRTPQWNELQPPFNANHPLLLAADAVQYYQFLLAGRIK
TEDSLNHSPGQSGFLSYGSSFSTSPTGQSPYTYQMHGTTGFYQGGNGLGNAAGFGSVHQDYPSYPGFPQSQYPQYYGSSYNPPYVPASSI
CPSPLSTSTYVLQEASHNVPNQSSESLAGEYNTHNGPSTPAKEGDTDRPHRASDGKLRGRSKRSSDPSPAGDNEIERVFVWDLDETIIIF
HSLLTGTFASRYGKDTTTSVRIGLMMEEMIFNLADTHLFFNDLEDCDQIHVDDVSSDDNGQDLSTYNFSADGFHSSAPGANLCLGSGVHG

--------------------------------------------------------------
>In-frame_ENST00000588874_ENST00000327619_TCGA-UL-AAZ6-01A_BCAS3_chr17_59118253_+_EYA2_chr20_45644820_length(transcript)=3560nt_BP=1530nt
GCCGCCTTGCAGTTTGATCTCAGACTGCTGTGCTGGCAATCAGCGAGACTCCGTGGGCGTAGGACCCTCCGAGCCAGGTGTGGGATATAG
TCTCGTGGTGCGCCGTTTTTTAAGCCGGTCTGAAAAGCGCAATATTCGGGTGGGAGTGACCCGATTTTCCAGGCTGCTATCCATGTCCAG
GGCCAAACATGAATCCTATTGCTCTTGGGAGCCGCTGGCTTGCTTATGCAGAAAACAAGTTGATTCGATGTCATCAGTCCCGTGGTGGAG
CCTGTGGAGACAACATTCAGTCTTATACTGCCACAGTCATTAGTGCTGCTAAAACATTGAAAAGTGGCCTGACAATGGTAGGGAAAGTGG
TGACTCAGCTGACAGGCACACTGCCTTCAGGTGTGACAGAAGATGATGTTGCCATCCACAGTAATTCACGGCGGAGTCCTTTGGTCCCAG
GCATCATCACAGTTATTGACACCGAAACCGTTGGAGAGGGCCAGGTGCTTGTGAGTGAGGATTCTGACAGTGATGGCATTGTGGCCCACT
TCCCTGCCCATGAGAAGCCAGTGTGCTGCATGGCTTTTAATACAAGTGGAATGCTTCTAGTCACAACAGACACCCTTGGCCATGACTTTC
ATGTCTTCCAAATTCTGACTCATCCTTGGTCCTCATCACAATGTGCTGTCCACCATCTGTATACTCTTCACAGGGGAGAAACTGAAGCCA
AAGTACAGGACATCTGCTTCAGCCATGACTGTCGCTGGGTTGTGGTCAGTACTCTCCGGGGTACTTCCCACGTTTTCCCCATCAACCCTT
ATGGTGGCCAGCCTTGTGTTCGTACACATATGTCACCACGAGTAGTGAATCGCATGAGCCGTTTCCAGAAAAGTGCTGGACTGGAAGAGA
TTGAACAAGAACTGACGTCTAAGCAAGGAGGTCGCTGTAGCCCTGTTCCAGGTCTATCAAGCAGCCCTTCTGGGTCACCCTTGCATGGGA
AACTGAACAGCCAAGACTCCTATAACAATTTTACCAACAACAACCCTGGCAACCCTCGGCTCTCTCCTCTTCCCAGCTTGATGGTAGTGA
TGCCTCTTGCACAAATCAAGCAGCCAATGACATTGGGGACCATCACCAAACGAACCGGCAAAGTTAAACCTCCTCCACAAATTTCACCCA
GCAAATCGATGGGCGGAGAATTTTGTGTGGCTGCTATCTTCGGAACATCCAGGTCATGGTTTGCAAATAATGCAGGTCTGAAAAGAGAAA
AAGATCAGTCCAAACAAGTTGTAGTTGAGTCCCTGTACATTATCAGTTGCTATGGCACCTTAGTGGAACACATGATGGAGCCGCGACCCC
TCAGCACTGCACCCAAGATTAGTGACGACACACCACTGGAAATGATGACATCGCCTCGAGCCAGCTGGACTCTGGTTAGAACCCCTCAAT
GGAATGAATTGCAGCCACCGTTTAATGCAAACCACCCTCTGCTCCTCGCTGCAGATGCAGTACAGTATTATCAGTTCCTGCTTGCTGGCC
GCATCAAGACAGAAGACAGCTTGAACCATTCCCCTGGCCAGAGTGGATTCCTCAGCTATGGCTCCAGCTTCAGCACCTCACCCACTGGAC
AGAGCCCATACACCTACCAGATGCACGGCACAACAGGGTTCTATCAAGGAGGAAATGGACTGGGCAACGCAGCCGGTTTCGGGAGTGTGC
ACCAGGACTATCCTTCCTACCCCGGCTTCCCCCAGAGCCAGTACCCCCAGTATTACGGCTCATCCTACAACCCTCCCTACGTCCCGGCCA
GCAGCATCTGCCCTTCGCCCCTCTCCACGTCCACCTACGTCCTCCAGGAGGCATCTCACAACGTCCCCAACCAGAGTTCCGAGTCACTTG
CTGGTGAATACAACACACACAATGGACCTTCCACACCAGCGAAAGAGGGAGACACAGACAGGCCGCACCGGGCCTCCGACGGGAAGCTCC
GAGGCCGGTCTAAGAGGAGCAGTGACCCGTCCCCGGCAGGGGACAATGAGATTGAGCGTGTGTTCGTGTGGGACTTGGATGAGACAATAA
TTATTTTTCACTCCTTACTCACGGGGACATTTGCATCCAGATACGGGAAGGACACCACGACGTCCGTGCGCATTGGCCTTATGATGGAAG
AGATGATCTTCAACCTTGCAGATACACATCTGTTCTTCAATGACCTGGAGGATTGTGACCAGATCCACGTTGATGACGTCTCATCAGATG
ACAATGGCCAAGATTTAAGCACATACAACTTCTCCGCTGACGGCTTCCACAGTTCGGCCCCAGGAGCCAACCTGTGCCTGGGCTCTGGCG
TGCACGGCGGCGTGGACTGGATGAGGAAGCTGGCCTTCCGCTACCGGCGGGTGAAGGAGATGTACAATACCTACAAGAACAACGTTGGTG
GGTTGATAGGCACTCCCAAAAGGGAGACCTGGCTACAGCTCCGAGCTGAGCTGGAAGCTCTCACAGACCTCTGGCTGACCCACTCCCTGA
AGGCACTAAACCTCATCAACTCCCGGCCCAACTGTGTCAATGTGCTGGTCACCACCACTCAACTAATTCCTGCCCTGGCCAAAGTCCTGC
TATATGGCCTGGGGTCTGTGTTTCCTATTGAGAACATCTACAGTGCAACCAAGACAGGGAAGGAGAGCTGCTTCGAGAGGATAATGCAGA
GATTCGGCAGAAAAGCTGTCTACGTGGTGATCGGTGATGGTGTGGAAGAGGAGCAAGGAGCGAAAAAGCACAACATGCCTTTCTGGCGGA
TATCCTGCCACGCAGACCTGGAGGCACTGAGGCACGCCCTGGAGCTGGAGTATTTATAGCAGGATCAGCAGCATCTCCACCTGCCATCTC
ACCCTCAGACCCCCTCGCCTTCCCCACCTCCCCACCGAGAACTCCAGAGACCCAGATGTTGGACACCAGGAAGGGGCCCCACAGCCGAGA
CGACGTGTCCAGTGACCATCTCAGAAGCCGTCCATCAGTCCAAATGGGGGTTCTGAGAAGGAAAGTACCCAACATTGGCTTCGGAGTATT
TGACTTTGGGGAAAAGGGCTGGCTCGGAGTCTAGACTCTTCTGTAAGACTCACAGAACAAAAGCAAGGAATTGCTGATTTGGGGGGTGCC
TGGTGATGAGGAGGGGATGGGTTTGTCTTGTCTTCTTTTTAATTTATGGACTAGTCTCATTACTCCGGAATTATGCTCTTGTACCTGTGT
GGCTGGGTTTCTTAGTCGTTGGTTTGGTTTGGTTTTTTGAACTGGTATGTGGGGTGGTTCACAGTTCTAATGTAAGCACTCTATTCTCCA
AGTTGTGCTTTGTGGGGACAATCATTCTTTGAACATTAGAGAGGAAGGCAGTTCAAGCTGTTGAAAAGACTATTGCTTATTTTTGTTTTT
AAAGACCTACTTGACGTCATGTGGACAGTGCACGTGCCTTACGCTACATCTTGTTTTCTAGGAAGAGGGGGATGCTGGGAAGGAATGGGT

>In-frame_ENST00000588874_ENST00000327619_TCGA-UL-AAZ6-01A_BCAS3_chr17_59118253_+_EYA2_chr20_45644820_length(amino acids)=886AA_start in transcript=188_stop in transcript=2848
MNPIALGSRWLAYAENKLIRCHQSRGGACGDNIQSYTATVISAAKTLKSGLTMVGKVVTQLTGTLPSGVTEDDVAIHSNSRRSPLVPGII
TVIDTETVGEGQVLVSEDSDSDGIVAHFPAHEKPVCCMAFNTSGMLLVTTDTLGHDFHVFQILTHPWSSSQCAVHHLYTLHRGETEAKVQ
DICFSHDCRWVVVSTLRGTSHVFPINPYGGQPCVRTHMSPRVVNRMSRFQKSAGLEEIEQELTSKQGGRCSPVPGLSSSPSGSPLHGKLN
SQDSYNNFTNNNPGNPRLSPLPSLMVVMPLAQIKQPMTLGTITKRTGKVKPPPQISPSKSMGGEFCVAAIFGTSRSWFANNAGLKREKDQ
SKQVVVESLYIISCYGTLVEHMMEPRPLSTAPKISDDTPLEMMTSPRASWTLVRTPQWNELQPPFNANHPLLLAADAVQYYQFLLAGRIK
TEDSLNHSPGQSGFLSYGSSFSTSPTGQSPYTYQMHGTTGFYQGGNGLGNAAGFGSVHQDYPSYPGFPQSQYPQYYGSSYNPPYVPASSI
CPSPLSTSTYVLQEASHNVPNQSSESLAGEYNTHNGPSTPAKEGDTDRPHRASDGKLRGRSKRSSDPSPAGDNEIERVFVWDLDETIIIF
HSLLTGTFASRYGKDTTTSVRIGLMMEEMIFNLADTHLFFNDLEDCDQIHVDDVSSDDNGQDLSTYNFSADGFHSSAPGANLCLGSGVHG
GVDWMRKLAFRYRRVKEMYNTYKNNVGGLIGTPKRETWLQLRAELEALTDLWLTHSLKALNLINSRPNCVNVLVTTTQLIPALAKVLLYG

--------------------------------------------------------------

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Fusion Gene PPI Analysis for BCAS3-EYA2


check button Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in

ChiPPI page.


check button Protein-protein interactors with each fusion partner protein in wild-type (BIOGRID-3.4.160)
HgeneHgene's interactorsTgeneTgene's interactors


check button - Retained PPIs in in-frame fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenStill interaction with


check button - Lost PPIs in in-frame fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenInteraction lost with


check button - Retained PPIs, but lost function due to frame-shift fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenInteraction lost with


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Related Drugs for BCAS3-EYA2


check button Drugs targeting genes involved in this fusion gene.
(DrugBank Version 5.1.8 2021-05-08)
PartnerGeneUniProtAccDrugBank IDDrug nameDrug activityDrug typeDrug status

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Related Diseases for BCAS3-EYA2


check button Diseases associated with fusion partners.
(DisGeNet 4.0)
PartnerGeneDisease IDDisease name# pubmedsSource