FusionGDB Logo

Home

Download

Statistics

Examples

Help

Contact
Center for Computational Systems Medicine
leaf

Fusion Gene Summary

leaf

Fusion Gene ORF analysis

leaf

Fusion Genomic Features

leaf

Fusion Protein Features

leaf

Fusion Gene Sequence

leaf

Fusion Gene PPI analysis

leaf

Related Drugs

leaf

Related Diseases

Fusion gene:UBE2K-FAM216B (FusionGDB2 ID:95491)

Fusion Gene Summary for UBE2K-FAM216B

check button Fusion gene summary
Fusion gene informationFusion gene name: UBE2K-FAM216B
Fusion gene ID: 95491
HgeneTgene
Gene symbol

UBE2K

FAM216B

Gene ID

3093

144809

Gene nameubiquitin conjugating enzyme E2 Kfamily with sequence similarity 216 member B
SynonymsE2-25K|HIP2|HYPG|LIG|UBC1C13orf30
Cytomap

4p14

13q14.11

Type of geneprotein-codingprotein-coding
Descriptionubiquitin-conjugating enzyme E2 KE2 ubiquitin-conjugating enzyme KE2(25K)HIP-2huntingtin-interacting protein 2ubiquitin carrier proteinubiquitin conjugating enzyme E2Kubiquitin-conjugating enzyme E2(25K)ubiquitin-conjugating enzyme E2-25 KDAubiquprotein FAM216B
Modification date2020031320200313
UniProtAcc..
Ensembl transtripts involved in fusion geneENST00000261427, ENST00000295963, 
ENST00000438068, ENST00000503368, 
ENST00000445950, 		ENST00000537894, 
ENST00000313851, 
Fusion gene scores* DoF score13 X 7 X 10=9101 X 2 X 1=2
# samples 172
** MAII scorelog2(17/910*10)=-2.42033179894836
possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs).
DoF>8 and MAII<0		log2(2/2*10)=3.32192809488736
Context

PubMed: UBE2K [Title/Abstract] AND FAM216B [Title/Abstract] AND fusion [Title/Abstract]

Most frequent breakpointUBE2K(39700010)-FAM216B(43360899), # samples:2
Anticipated loss of major functional domain due to fusion event.
* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

check button Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez
PartnerGeneGO IDGO termPubMed ID
HgeneUBE2K

GO:0010800

positive regulation of peptidyl-threonine phosphorylation

24882218

HgeneUBE2K

GO:0010994

free ubiquitin chain polymerization

24882218

HgeneUBE2K

GO:0070936

protein K48-linked ubiquitination

20061386


check buttonFusion gene breakpoints across UBE2K (5'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

check buttonFusion gene breakpoints across FAM216B (3'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

check button Fusion gene information from two resources (ChiTars 5.0 and ChimerDB 4.0)
* All genome coordinats were lifted-over on hg19.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.
SourceDiseaseSampleHgeneHchrHbpHstrandTgeneTchrTbpTstrand
ChimerDB4LUADTCGA-86-A4P7-01AUBE2Kchr4

39700010

+FAM216Bchr13

43360899

+
ChimerDB4LUADTCGA-86-A4P7UBE2Kchr4

39700010

+FAM216Bchr13

43360898

+
ChimerDB4LUADTCGA-86-A4P7-01AUBE2Kchr4

39700010

+FAM216Bchr13

43360899

+


Top

Fusion Gene ORF analysis for UBE2K-FAM216B

check button Open reading frame (ORF) analsis of fusion genes based on Ensembl gene isoform structure.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.
ORFHenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrand
In-frameENST00000261427ENST00000537894UBE2Kchr4

39700010

+FAM216Bchr13

43360899

+
In-frameENST00000261427ENST00000313851UBE2Kchr4

39700010

+FAM216Bchr13

43360899

+
In-frameENST00000295963ENST00000537894UBE2Kchr4

39700010

+FAM216Bchr13

43360899

+
In-frameENST00000295963ENST00000313851UBE2Kchr4

39700010

+FAM216Bchr13

43360899

+
intron-3CDSENST00000438068ENST00000537894UBE2Kchr4

39700010

+FAM216Bchr13

43360899

+
intron-3CDSENST00000438068ENST00000313851UBE2Kchr4

39700010

+FAM216Bchr13

43360899

+
In-frameENST00000503368ENST00000537894UBE2Kchr4

39700010

+FAM216Bchr13

43360899

+
In-frameENST00000503368ENST00000313851UBE2Kchr4

39700010

+FAM216Bchr13

43360899

+
In-frameENST00000445950ENST00000537894UBE2Kchr4

39700010

+FAM216Bchr13

43360899

+
In-frameENST00000445950ENST00000313851UBE2Kchr4

39700010

+FAM216Bchr13

43360899

+
In-frameENST00000261427ENST00000537894UBE2Kchr4

39700010

+FAM216Bchr13

43360898

+
In-frameENST00000261427ENST00000313851UBE2Kchr4

39700010

+FAM216Bchr13

43360898

+
In-frameENST00000295963ENST00000537894UBE2Kchr4

39700010

+FAM216Bchr13

43360898

+
In-frameENST00000295963ENST00000313851UBE2Kchr4

39700010

+FAM216Bchr13

43360898

+
intron-3CDSENST00000438068ENST00000537894UBE2Kchr4

39700010

+FAM216Bchr13

43360898

+
intron-3CDSENST00000438068ENST00000313851UBE2Kchr4

39700010

+FAM216Bchr13

43360898

+
In-frameENST00000503368ENST00000537894UBE2Kchr4

39700010

+FAM216Bchr13

43360898

+
In-frameENST00000503368ENST00000313851UBE2Kchr4

39700010

+FAM216Bchr13

43360898

+
In-frameENST00000445950ENST00000537894UBE2Kchr4

39700010

+FAM216Bchr13

43360898

+
In-frameENST00000445950ENST00000313851UBE2Kchr4

39700010

+FAM216Bchr13

43360898

+

check buttonORFfinder result based on the fusion transcript sequence of in-frame fusion genes.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandSeq length
(transcript)		BP loci
(transcript)		Predicted start
(transcript)		Predicted stop
(transcript)		Seq length
(amino acids)
ENST00000261427UBE2Kchr439700010+ENST00000537894FAM216Bchr1343360899+3425347284667127
ENST00000261427UBE2Kchr439700010+ENST00000313851FAM216Bchr1343360899+3425347284667127
ENST00000295963UBE2Kchr439700010+ENST00000537894FAM216Bchr1343360899+3360282219602127
ENST00000295963UBE2Kchr439700010+ENST00000313851FAM216Bchr1343360899+3360282219602127
ENST00000503368UBE2Kchr439700010+ENST00000537894FAM216Bchr1343360899+3306228165548127
ENST00000503368UBE2Kchr439700010+ENST00000313851FAM216Bchr1343360899+3306228165548127
ENST00000445950UBE2Kchr439700010+ENST00000537894FAM216Bchr1343360899+3293215152535127
ENST00000445950UBE2Kchr439700010+ENST00000313851FAM216Bchr1343360899+3293215152535127
ENST00000261427UBE2Kchr439700010+ENST00000537894FAM216Bchr1343360898+3425347284667127
ENST00000261427UBE2Kchr439700010+ENST00000313851FAM216Bchr1343360898+3425347284667127
ENST00000295963UBE2Kchr439700010+ENST00000537894FAM216Bchr1343360898+3360282219602127
ENST00000295963UBE2Kchr439700010+ENST00000313851FAM216Bchr1343360898+3360282219602127
ENST00000503368UBE2Kchr439700010+ENST00000537894FAM216Bchr1343360898+3306228165548127
ENST00000503368UBE2Kchr439700010+ENST00000313851FAM216Bchr1343360898+3306228165548127
ENST00000445950UBE2Kchr439700010+ENST00000537894FAM216Bchr1343360898+3293215152535127
ENST00000445950UBE2Kchr439700010+ENST00000313851FAM216Bchr1343360898+3293215152535127

check buttonDeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandNo-coding scoreCoding score
ENST00000261427ENST00000537894UBE2Kchr439700010+FAM216Bchr1343360899+0.0159069430.98409307
ENST00000261427ENST00000313851UBE2Kchr439700010+FAM216Bchr1343360899+0.0159069430.98409307
ENST00000295963ENST00000537894UBE2Kchr439700010+FAM216Bchr1343360899+0.016050160.9839498
ENST00000295963ENST00000313851UBE2Kchr439700010+FAM216Bchr1343360899+0.016050160.9839498
ENST00000503368ENST00000537894UBE2Kchr439700010+FAM216Bchr1343360899+0.0166171530.9833829
ENST00000503368ENST00000313851UBE2Kchr439700010+FAM216Bchr1343360899+0.0166171530.9833829
ENST00000445950ENST00000537894UBE2Kchr439700010+FAM216Bchr1343360899+0.016800580.98319936
ENST00000445950ENST00000313851UBE2Kchr439700010+FAM216Bchr1343360899+0.016800580.98319936
ENST00000261427ENST00000537894UBE2Kchr439700010+FAM216Bchr1343360898+0.0159069430.98409307
ENST00000261427ENST00000313851UBE2Kchr439700010+FAM216Bchr1343360898+0.0159069430.98409307
ENST00000295963ENST00000537894UBE2Kchr439700010+FAM216Bchr1343360898+0.016050160.9839498
ENST00000295963ENST00000313851UBE2Kchr439700010+FAM216Bchr1343360898+0.016050160.9839498
ENST00000503368ENST00000537894UBE2Kchr439700010+FAM216Bchr1343360898+0.0166171530.9833829
ENST00000503368ENST00000313851UBE2Kchr439700010+FAM216Bchr1343360898+0.0166171530.9833829
ENST00000445950ENST00000537894UBE2Kchr439700010+FAM216Bchr1343360898+0.016800580.98319936
ENST00000445950ENST00000313851UBE2Kchr439700010+FAM216Bchr1343360898+0.016800580.98319936

Top

Fusion Genomic Features for UBE2K-FAM216B


check buttonFusionAI prediction of the potential fusion gene breakpoint based on the pre-mature RNA sequence context (+/- 5kb of individual partner genes, total 20kb length sequence). FusionAI is a fusion gene breakpoint classifier based on convolutional neural network by comparing the fusion positive and negative sequence context of ~ 20K fusion gene data. From here, we can have the relative potentency of the 20K genomic sequence how individual sequnce will be likely used as the gene fusion breakpoints.
HgeneHchrHbpHstrandTgeneTchrTbpTstrand1-pp (fusion gene breakpoint)
UBE2Kchr439700010+FAM216Bchr1343360898+4.90E-141
UBE2Kchr439700010+FAM216Bchr1343360898+4.90E-141
UBE2Kchr439700010+FAM216Bchr1343360898+4.90E-141
UBE2Kchr439700010+FAM216Bchr1343360898+4.90E-141

check buttonDistribution of 44 human genomic features loci across 20kb length fusion breakpoint regions. We integrated a total of 44 different types of human genomic feature loci information across five big categories including virus integration sites, repeats, structural variants, chromatin states, and gene expression regulation. More details are in help page.

check buttonDistribution of 44 human genomic features loci across 20kb length fusion breakpoint regions that are ovelapped with the top 1% feature importance score regions. More details are in help page.

Top

Fusion Protein Features for UBE2K-FAM216B


check button Go to

FGviewer for the breakpoints of chr4:39700010-chr13:43360899

.
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
FGviewer

check buttonMain function of each fusion partner protein. (from UniProt)
HgeneTgene
..
FUNCTION: Might normally function as a transcriptional repressor. EWS-fusion-proteins (EFPS) may play a role in the tumorigenic process. They may disturb gene expression by mimicking, or interfering with the normal function of CTD-POLII within the transcription initiation complex. They may also contribute to an aberrant activation of the fusion protein target genes.FUNCTION: Might normally function as a transcriptional repressor. EWS-fusion-proteins (EFPS) may play a role in the tumorigenic process. They may disturb gene expression by mimicking, or interfering with the normal function of CTD-POLII within the transcription initiation complex. They may also contribute to an aberrant activation of the fusion protein target genes.

check buttonRetention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at

download page


* Minus value of BPloci means that the break pointn is located before the CDS.
- In-frame and retained protein feature among the 13 regional features.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note

- In-frame and not-retained protein feature among the 13 regional features.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note
HgeneUBE2Kchr4:39700010chr13:43360898ENST00000261427+17160_20021.0201.0DomainUBA
HgeneUBE2Kchr4:39700010chr13:43360898ENST00000261427+174_15421.0201.0DomainUBC core
HgeneUBE2Kchr4:39700010chr13:43360898ENST00000445950+16160_20021.0158.0DomainUBA
HgeneUBE2Kchr4:39700010chr13:43360898ENST00000445950+164_15421.0158.0DomainUBC core
HgeneUBE2Kchr4:39700010chr13:43360898ENST00000503368+15160_20021.0150.0DomainUBA
HgeneUBE2Kchr4:39700010chr13:43360898ENST00000503368+154_15421.0150.0DomainUBC core
HgeneUBE2Kchr4:39700010chr13:43360899ENST00000261427+17160_20021.0201.0DomainUBA
HgeneUBE2Kchr4:39700010chr13:43360899ENST00000261427+174_15421.0201.0DomainUBC core
HgeneUBE2Kchr4:39700010chr13:43360899ENST00000445950+16160_20021.0158.0DomainUBA
HgeneUBE2Kchr4:39700010chr13:43360899ENST00000445950+164_15421.0158.0DomainUBC core
HgeneUBE2Kchr4:39700010chr13:43360899ENST00000503368+15160_20021.0150.0DomainUBA
HgeneUBE2Kchr4:39700010chr13:43360899ENST00000503368+154_15421.0150.0DomainUBC core


Top

Fusion Gene Sequence for UBE2K-FAM216B


check button For in-frame fusion transcripts, we provide the fusion transcript sequences and fusion amino acid sequences. To have fusion amino acid sequence, we ran ORFfinder and chose the longest ORF among the all predicted ones.
>In-frame_ENST00000261427_ENST00000537894_TCGA-86-A4P7-01A_UBE2K_chr4_39700010_+_FAM216B_chr13_43360899_length(transcript)=3425nt_BP=347nt
ACGCGAGCTCGTGGCTCGGGCGTATGGGGGCTCCAACGGGAGCGGCTGCGCCGCCCGGCCGGGTCAACGTCGTGCGCATGCGCCGACCCG
GCGCCATTTTGGTGGCCGGGCGCGGAGGTGATTCCACACTGAGGCGAGCGCGGCGGCCGGGGTGGTAGTGGCAGTGTTCGTGTGCTCAGG
TCTGAATCGCCGAGGGAGGAGGCGGTGGAGGAAGAGGTGGCGGCGGTGGCGGTGGTCGTAGCGGTGGCGGAGGAGGCGGGTACGAATCAG
CTGCGGGCGGAGACATGGCCAACATCGCGGTGCAGCGAATCAAGCGGGAGTTCAAGGAGGTGCTGAAGAGCGAGGAGGCCCTCAACCAAG
GGCAACAGCGCTACTTTTACAGCATTATGAGGATTTACAACTCCAGGCCCCAGTGGGAGGCCCTGCAGACCCGCTACATTCACAGCCTTC
AGCACCAACAGCTGCTTGGCTATATTACTCAACGGGAAGCCTTGTCTTATGCTCTTGTACTTAGAGATTCAACCAAGAGAGCCTCAGCCA
AGGTAGCTCCTCAAAGAACCATTCCCCGGAAAACTTCAGCCATGACAAGAAGATGTCCATCAGTACTACCTGTATCTGTGGTTCTACCTA
GGGCCCAAAGTAAAAGGCGCCAAGTGCTCAGGAACTGAGACTTGGCAGCATTTTCAGAAACAGGAAGTTTGCCCATGTATCCCTGGTATC
TAGTGGGTGCTTTGTGCATATTTGTTGAATGACAGTGAATAATTTCTAATATAAACCCCAGACCTAAAAATAATCTCTGATTCATATAAA
TTTTGCCAGCAAGACCAAGAGGTTTAAGAGCAATGGAGCCGAGAGTAAGCAAGCCTTTCCACATAACATCCGTAGAGTAAAAGGACTTTT
AAAGCTTTCAAAGTAAAAGTTTACTTTGGATTAGAACCTCCTAGAGAAGTCCCCAAAACAGTGAAAGGGGACAAGTGACCAGAAAGGGCC
AGGTCCTCTGTCACTGGGTGACCATTTCTAGCAAACTATCATGTATACATTTTGAAAATATACAAAACAACACAGGATTAACACTGATCA
TAGAGGGCTTACAAAATCATATCATCCCAGCAACAATTGGCCTGTGCATTTCCTTCCTCTCCTCTTCCCCATCAGGCAATGGCATTTAAT
TTAGCCCCAAAGCTCTTCTAGTTCTCACCTGGCTTGATCACTTTGTGTCATCTTGAAACATGCACTTATCAGTCTACCACAGCTGAATTG
ATTTTATGGAGCTGTAACTGGCCTTGACATGGGTAAGAGAATTCATGCACACATCTTTCCCTGGCAGCTGAAGAAACACTCTTAAAAGAA
TATGAAATAACCCCGAAGACTTTATTTCATGTCCAACCTTTCTGTGGCCTCAAATGTTTTAAGCAAAGCACATAAAACTATACTAAGCAG
TGAGTCAGTTCATCATACAAAGGCATAGTATTGCATTAGTTATATTTAATGGTTATTTTCATCACCAGAGATTTTGTGTGTGTGTGTGTG
TGTGTGTGTGTGTGTGTTAAAAATCAGTCACCAGTGCTCATGTAAAGTCAAAACAAAAAAATGCTTCTTCCTCATCATTGTAAAATACGT
CACCCCATTCAAATCAGCTGATGCCTATGAAAGGAATTGGTTGAGGTGGAATATAAATTTTATTGACAAGGGATGTGCTAACATTGATCT
GAAAGGAATATGCTGCATTATTATCAGAAAAAAAATCATTATCTTCTCTTTGCAATCATGAACATATAAGATATAAAAATTGTCATGAGA
TTCTGAACACTAGTATTGAGGTCCAGCACTACTTCCAAGCTCCTATCTGACTTTTCTTTGGGAGGAATGGGAAGTGTGGTTGGCCCACTA
GTCTGATAAGAAGTCTTGGTGAAGCTAATACTGACCTTGCTGGGGTCATTCACTTAATCATAAAGAAGCTTCTTGACCTCTGCAAATTCT
GGCCCTGACATGTATTCTAAATACCTTTACAGTTCTAAGTGAGAAATTGTTACATAGGCTTAGAAGAAGAAAAGACTCCAGTGGGACTGG
GATGAGCTGCGTGGTAGCCCTCATAAAGCAGAGAGGTTGGTGGGAGACCTAACTTGGCCTATACTAGCCTTCCCATTGTTTTCACAATGG
GCCTTTTATCCCCTCTGAACTGCTTGCCTGCACTTTTATCTTAGACTTTTTTTTTTGATGAGCATAGGTATTTGCTGTGTAAGCCTTATC
TAGTGATGCAGAATATCAAGGAGGCCACTTACAATTCTAAGCTGCATCCCTGTATCAAAAATCTCAGAAGCACCTTGCATCTGATAGAGG
CTTGTAAGAGCAGATTGACAGGTGAATGTCACAGGTCCATAAGAAGGACAAATAGATCTAAGTTGGGCAAAAGATTCTATTTGTTGGTTC
TCCCTTCTAAGATGATAATATGCCCCTGCACTTGAGACCTTACTTGAAACTTAAAGAAATGTGATTGGTTATGGTGCTGGGAGAAAGGGG
TGCACCTGGGAGGACACTGAGAAAGATAAACAAAGGGGAAGGAAGAGAGGACAACCTGTAACTTTTAGAAAAGGCTACTACTTTTAACTG
TTGGAGAAAATGCTATTGCTTGCACTCACAATGTCAATATACATTATCAGAACTTAAGTCCTGGAGATCAGAGACTGTTTCACCTGAAAT
TGCTAGCACCTAGAATGGCCCCCGGTTGCGGTAGGCAATCAATAATACTCTTTGAATAATAATGAGGTTTTCAAAGATTCTGCAAGTCAG
ACTGGCCTCTGGCTTTGTGATATTATTCTAGAGTCCCAGTAACCCACAATTGTTGATGAGGAGTAGCAGTAGAATATCAAGGACAGGTGA
TGAGACAACAAGTCTATCCCGCAAAGGGCAGGGAATAGAGGGTACACTGGAAAGAATTTGAAAACAATAATAAAGCTGACTACAAGTTGG
TCTATTTTTTATTACCACCATGAGCCAGCAATTCTAAATAATATCAGTGATAAAGTATTCATCCCCCAAAATATTTTATTGAACTAGGTT
CTAAACAATTTCTGAGGTTACTGTTGAGTTTTAAATAATACATATATGTAAGCTTCAAAGTAGCACATTTGTACTGTTTTTCTTCAATAA
TCATTTTACTCCACATGGAAACTAATTCAGGGAATATCCTGCTATATAGTTGGCTCTCAGTACATATGGTGTCAGCTCATGTGTCTCCAG
CCCCTGTAGTACTATATATTATTGCATTTAAATGATGGATTTGAAATAAACAATGATTATACCATGATTGTAAAGACAAGAAACAGAACC
TGAAATACTTCAATTTTTGATTCTATGTGGCAGTTTAGAGTTTTTTAAAACAATTTTTATGTATAATTTATATGCAATAAATGCCCTCAA

>In-frame_ENST00000261427_ENST00000537894_TCGA-86-A4P7-01A_UBE2K_chr4_39700010_+_FAM216B_chr13_43360899_length(amino acids)=127AA_start in transcript=284_stop in transcript=667
MANIAVQRIKREFKEVLKSEEALNQGQQRYFYSIMRIYNSRPQWEALQTRYIHSLQHQQLLGYITQREALSYALVLRDSTKRASAKVAPQ

--------------------------------------------------------------
>In-frame_ENST00000261427_ENST00000313851_TCGA-86-A4P7-01A_UBE2K_chr4_39700010_+_FAM216B_chr13_43360899_length(transcript)=3425nt_BP=347nt
ACGCGAGCTCGTGGCTCGGGCGTATGGGGGCTCCAACGGGAGCGGCTGCGCCGCCCGGCCGGGTCAACGTCGTGCGCATGCGCCGACCCG
GCGCCATTTTGGTGGCCGGGCGCGGAGGTGATTCCACACTGAGGCGAGCGCGGCGGCCGGGGTGGTAGTGGCAGTGTTCGTGTGCTCAGG
TCTGAATCGCCGAGGGAGGAGGCGGTGGAGGAAGAGGTGGCGGCGGTGGCGGTGGTCGTAGCGGTGGCGGAGGAGGCGGGTACGAATCAG
CTGCGGGCGGAGACATGGCCAACATCGCGGTGCAGCGAATCAAGCGGGAGTTCAAGGAGGTGCTGAAGAGCGAGGAGGCCCTCAACCAAG
GGCAACAGCGCTACTTTTACAGCATTATGAGGATTTACAACTCCAGGCCCCAGTGGGAGGCCCTGCAGACCCGCTACATTCACAGCCTTC
AGCACCAACAGCTGCTTGGCTATATTACTCAACGGGAAGCCTTGTCTTATGCTCTTGTACTTAGAGATTCAACCAAGAGAGCCTCAGCCA
AGGTAGCTCCTCAAAGAACCATTCCCCGGAAAACTTCAGCCATGACAAGAAGATGTCCATCAGTACTACCTGTATCTGTGGTTCTACCTA
GGGCCCAAAGTAAAAGGCGCCAAGTGCTCAGGAACTGAGACTTGGCAGCATTTTCAGAAACAGGAAGTTTGCCCATGTATCCCTGGTATC
TAGTGGGTGCTTTGTGCATATTTGTTGAATGACAGTGAATAATTTCTAATATAAACCCCAGACCTAAAAATAATCTCTGATTCATATAAA
TTTTGCCAGCAAGACCAAGAGGTTTAAGAGCAATGGAGCCGAGAGTAAGCAAGCCTTTCCACATAACATCCGTAGAGTAAAAGGACTTTT
AAAGCTTTCAAAGTAAAAGTTTACTTTGGATTAGAACCTCCTAGAGAAGTCCCCAAAACAGTGAAAGGGGACAAGTGACCAGAAAGGGCC
AGGTCCTCTGTCACTGGGTGACCATTTCTAGCAAACTATCATGTATACATTTTGAAAATATACAAAACAACACAGGATTAACACTGATCA
TAGAGGGCTTACAAAATCATATCATCCCAGCAACAATTGGCCTGTGCATTTCCTTCCTCTCCTCTTCCCCATCAGGCAATGGCATTTAAT
TTAGCCCCAAAGCTCTTCTAGTTCTCACCTGGCTTGATCACTTTGTGTCATCTTGAAACATGCACTTATCAGTCTACCACAGCTGAATTG
ATTTTATGGAGCTGTAACTGGCCTTGACATGGGTAAGAGAATTCATGCACACATCTTTCCCTGGCAGCTGAAGAAACACTCTTAAAAGAA
TATGAAATAACCCCGAAGACTTTATTTCATGTCCAACCTTTCTGTGGCCTCAAATGTTTTAAGCAAAGCACATAAAACTATACTAAGCAG
TGAGTCAGTTCATCATACAAAGGCATAGTATTGCATTAGTTATATTTAATGGTTATTTTCATCACCAGAGATTTTGTGTGTGTGTGTGTG
TGTGTGTGTGTGTGTGTTAAAAATCAGTCACCAGTGCTCATGTAAAGTCAAAACAAAAAAATGCTTCTTCCTCATCATTGTAAAATACGT
CACCCCATTCAAATCAGCTGATGCCTATGAAAGGAATTGGTTGAGGTGGAATATAAATTTTATTGACAAGGGATGTGCTAACATTGATCT
GAAAGGAATATGCTGCATTATTATCAGAAAAAAAATCATTATCTTCTCTTTGCAATCATGAACATATAAGATATAAAAATTGTCATGAGA
TTCTGAACACTAGTATTGAGGTCCAGCACTACTTCCAAGCTCCTATCTGACTTTTCTTTGGGAGGAATGGGAAGTGTGGTTGGCCCACTA
GTCTGATAAGAAGTCTTGGTGAAGCTAATACTGACCTTGCTGGGGTCATTCACTTAATCATAAAGAAGCTTCTTGACCTCTGCAAATTCT
GGCCCTGACATGTATTCTAAATACCTTTACAGTTCTAAGTGAGAAATTGTTACATAGGCTTAGAAGAAGAAAAGACTCCAGTGGGACTGG
GATGAGCTGCGTGGTAGCCCTCATAAAGCAGAGAGGTTGGTGGGAGACCTAACTTGGCCTATACTAGCCTTCCCATTGTTTTCACAATGG
GCCTTTTATCCCCTCTGAACTGCTTGCCTGCACTTTTATCTTAGACTTTTTTTTTTGATGAGCATAGGTATTTGCTGTGTAAGCCTTATC
TAGTGATGCAGAATATCAAGGAGGCCACTTACAATTCTAAGCTGCATCCCTGTATCAAAAATCTCAGAAGCACCTTGCATCTGATAGAGG
CTTGTAAGAGCAGATTGACAGGTGAATGTCACAGGTCCATAAGAAGGACAAATAGATCTAAGTTGGGCAAAAGATTCTATTTGTTGGTTC
TCCCTTCTAAGATGATAATATGCCCCTGCACTTGAGACCTTACTTGAAACTTAAAGAAATGTGATTGGTTATGGTGCTGGGAGAAAGGGG
TGCACCTGGGAGGACACTGAGAAAGATAAACAAAGGGGAAGGAAGAGAGGACAACCTGTAACTTTTAGAAAAGGCTACTACTTTTAACTG
TTGGAGAAAATGCTATTGCTTGCACTCACAATGTCAATATACATTATCAGAACTTAAGTCCTGGAGATCAGAGACTGTTTCACCTGAAAT
TGCTAGCACCTAGAATGGCCCCCGGTTGCGGTAGGCAATCAATAATACTCTTTGAATAATAATGAGGTTTTCAAAGATTCTGCAAGTCAG
ACTGGCCTCTGGCTTTGTGATATTATTCTAGAGTCCCAGTAACCCACAATTGTTGATGAGGAGTAGCAGTAGAATATCAAGGACAGGTGA
TGAGACAACAAGTCTATCCCGCAAAGGGCAGGGAATAGAGGGTACACTGGAAAGAATTTGAAAACAATAATAAAGCTGACTACAAGTTGG
TCTATTTTTTATTACCACCATGAGCCAGCAATTCTAAATAATATCAGTGATAAAGTATTCATCCCCCAAAATATTTTATTGAACTAGGTT
CTAAACAATTTCTGAGGTTACTGTTGAGTTTTAAATAATACATATATGTAAGCTTCAAAGTAGCACATTTGTACTGTTTTTCTTCAATAA
TCATTTTACTCCACATGGAAACTAATTCAGGGAATATCCTGCTATATAGTTGGCTCTCAGTACATATGGTGTCAGCTCATGTGTCTCCAG
CCCCTGTAGTACTATATATTATTGCATTTAAATGATGGATTTGAAATAAACAATGATTATACCATGATTGTAAAGACAAGAAACAGAACC
TGAAATACTTCAATTTTTGATTCTATGTGGCAGTTTAGAGTTTTTTAAAACAATTTTTATGTATAATTTATATGCAATAAATGCCCTCAA

>In-frame_ENST00000261427_ENST00000313851_TCGA-86-A4P7-01A_UBE2K_chr4_39700010_+_FAM216B_chr13_43360899_length(amino acids)=127AA_start in transcript=284_stop in transcript=667
MANIAVQRIKREFKEVLKSEEALNQGQQRYFYSIMRIYNSRPQWEALQTRYIHSLQHQQLLGYITQREALSYALVLRDSTKRASAKVAPQ

--------------------------------------------------------------
>In-frame_ENST00000295963_ENST00000537894_TCGA-86-A4P7-01A_UBE2K_chr4_39700010_+_FAM216B_chr13_43360899_length(transcript)=3360nt_BP=282nt
AACGTCGTGCGCATGCGCCGACCCGGCGCCATTTTGGTGGCCGGGCGCGGAGGTGATTCCACACTGAGGCGAGCGCGGCGGCCGGGGTGG
TAGTGGCAGTGTTCGTGTGCTCAGGTCTGAATCGCCGAGGGAGGAGGCGGTGGAGGAAGAGGTGGCGGCGGTGGCGGTGGTCGTAGCGGT
GGCGGAGGAGGCGGGTACGAATCAGCTGCGGGCGGAGACATGGCCAACATCGCGGTGCAGCGAATCAAGCGGGAGTTCAAGGAGGTGCTG
AAGAGCGAGGAGGCCCTCAACCAAGGGCAACAGCGCTACTTTTACAGCATTATGAGGATTTACAACTCCAGGCCCCAGTGGGAGGCCCTG
CAGACCCGCTACATTCACAGCCTTCAGCACCAACAGCTGCTTGGCTATATTACTCAACGGGAAGCCTTGTCTTATGCTCTTGTACTTAGA
GATTCAACCAAGAGAGCCTCAGCCAAGGTAGCTCCTCAAAGAACCATTCCCCGGAAAACTTCAGCCATGACAAGAAGATGTCCATCAGTA
CTACCTGTATCTGTGGTTCTACCTAGGGCCCAAAGTAAAAGGCGCCAAGTGCTCAGGAACTGAGACTTGGCAGCATTTTCAGAAACAGGA
AGTTTGCCCATGTATCCCTGGTATCTAGTGGGTGCTTTGTGCATATTTGTTGAATGACAGTGAATAATTTCTAATATAAACCCCAGACCT
AAAAATAATCTCTGATTCATATAAATTTTGCCAGCAAGACCAAGAGGTTTAAGAGCAATGGAGCCGAGAGTAAGCAAGCCTTTCCACATA
ACATCCGTAGAGTAAAAGGACTTTTAAAGCTTTCAAAGTAAAAGTTTACTTTGGATTAGAACCTCCTAGAGAAGTCCCCAAAACAGTGAA
AGGGGACAAGTGACCAGAAAGGGCCAGGTCCTCTGTCACTGGGTGACCATTTCTAGCAAACTATCATGTATACATTTTGAAAATATACAA
AACAACACAGGATTAACACTGATCATAGAGGGCTTACAAAATCATATCATCCCAGCAACAATTGGCCTGTGCATTTCCTTCCTCTCCTCT
TCCCCATCAGGCAATGGCATTTAATTTAGCCCCAAAGCTCTTCTAGTTCTCACCTGGCTTGATCACTTTGTGTCATCTTGAAACATGCAC
TTATCAGTCTACCACAGCTGAATTGATTTTATGGAGCTGTAACTGGCCTTGACATGGGTAAGAGAATTCATGCACACATCTTTCCCTGGC
AGCTGAAGAAACACTCTTAAAAGAATATGAAATAACCCCGAAGACTTTATTTCATGTCCAACCTTTCTGTGGCCTCAAATGTTTTAAGCA
AAGCACATAAAACTATACTAAGCAGTGAGTCAGTTCATCATACAAAGGCATAGTATTGCATTAGTTATATTTAATGGTTATTTTCATCAC
CAGAGATTTTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTTAAAAATCAGTCACCAGTGCTCATGTAAAGTCAAAACAAAAAAATGCT
TCTTCCTCATCATTGTAAAATACGTCACCCCATTCAAATCAGCTGATGCCTATGAAAGGAATTGGTTGAGGTGGAATATAAATTTTATTG
ACAAGGGATGTGCTAACATTGATCTGAAAGGAATATGCTGCATTATTATCAGAAAAAAAATCATTATCTTCTCTTTGCAATCATGAACAT
ATAAGATATAAAAATTGTCATGAGATTCTGAACACTAGTATTGAGGTCCAGCACTACTTCCAAGCTCCTATCTGACTTTTCTTTGGGAGG
AATGGGAAGTGTGGTTGGCCCACTAGTCTGATAAGAAGTCTTGGTGAAGCTAATACTGACCTTGCTGGGGTCATTCACTTAATCATAAAG
AAGCTTCTTGACCTCTGCAAATTCTGGCCCTGACATGTATTCTAAATACCTTTACAGTTCTAAGTGAGAAATTGTTACATAGGCTTAGAA
GAAGAAAAGACTCCAGTGGGACTGGGATGAGCTGCGTGGTAGCCCTCATAAAGCAGAGAGGTTGGTGGGAGACCTAACTTGGCCTATACT
AGCCTTCCCATTGTTTTCACAATGGGCCTTTTATCCCCTCTGAACTGCTTGCCTGCACTTTTATCTTAGACTTTTTTTTTTGATGAGCAT
AGGTATTTGCTGTGTAAGCCTTATCTAGTGATGCAGAATATCAAGGAGGCCACTTACAATTCTAAGCTGCATCCCTGTATCAAAAATCTC
AGAAGCACCTTGCATCTGATAGAGGCTTGTAAGAGCAGATTGACAGGTGAATGTCACAGGTCCATAAGAAGGACAAATAGATCTAAGTTG
GGCAAAAGATTCTATTTGTTGGTTCTCCCTTCTAAGATGATAATATGCCCCTGCACTTGAGACCTTACTTGAAACTTAAAGAAATGTGAT
TGGTTATGGTGCTGGGAGAAAGGGGTGCACCTGGGAGGACACTGAGAAAGATAAACAAAGGGGAAGGAAGAGAGGACAACCTGTAACTTT
TAGAAAAGGCTACTACTTTTAACTGTTGGAGAAAATGCTATTGCTTGCACTCACAATGTCAATATACATTATCAGAACTTAAGTCCTGGA
GATCAGAGACTGTTTCACCTGAAATTGCTAGCACCTAGAATGGCCCCCGGTTGCGGTAGGCAATCAATAATACTCTTTGAATAATAATGA
GGTTTTCAAAGATTCTGCAAGTCAGACTGGCCTCTGGCTTTGTGATATTATTCTAGAGTCCCAGTAACCCACAATTGTTGATGAGGAGTA
GCAGTAGAATATCAAGGACAGGTGATGAGACAACAAGTCTATCCCGCAAAGGGCAGGGAATAGAGGGTACACTGGAAAGAATTTGAAAAC
AATAATAAAGCTGACTACAAGTTGGTCTATTTTTTATTACCACCATGAGCCAGCAATTCTAAATAATATCAGTGATAAAGTATTCATCCC
CCAAAATATTTTATTGAACTAGGTTCTAAACAATTTCTGAGGTTACTGTTGAGTTTTAAATAATACATATATGTAAGCTTCAAAGTAGCA
CATTTGTACTGTTTTTCTTCAATAATCATTTTACTCCACATGGAAACTAATTCAGGGAATATCCTGCTATATAGTTGGCTCTCAGTACAT
ATGGTGTCAGCTCATGTGTCTCCAGCCCCTGTAGTACTATATATTATTGCATTTAAATGATGGATTTGAAATAAACAATGATTATACCAT
GATTGTAAAGACAAGAAACAGAACCTGAAATACTTCAATTTTTGATTCTATGTGGCAGTTTAGAGTTTTTTAAAACAATTTTTATGTATA

>In-frame_ENST00000295963_ENST00000537894_TCGA-86-A4P7-01A_UBE2K_chr4_39700010_+_FAM216B_chr13_43360899_length(amino acids)=127AA_start in transcript=219_stop in transcript=602
MANIAVQRIKREFKEVLKSEEALNQGQQRYFYSIMRIYNSRPQWEALQTRYIHSLQHQQLLGYITQREALSYALVLRDSTKRASAKVAPQ

--------------------------------------------------------------
>In-frame_ENST00000295963_ENST00000313851_TCGA-86-A4P7-01A_UBE2K_chr4_39700010_+_FAM216B_chr13_43360899_length(transcript)=3360nt_BP=282nt
AACGTCGTGCGCATGCGCCGACCCGGCGCCATTTTGGTGGCCGGGCGCGGAGGTGATTCCACACTGAGGCGAGCGCGGCGGCCGGGGTGG
TAGTGGCAGTGTTCGTGTGCTCAGGTCTGAATCGCCGAGGGAGGAGGCGGTGGAGGAAGAGGTGGCGGCGGTGGCGGTGGTCGTAGCGGT
GGCGGAGGAGGCGGGTACGAATCAGCTGCGGGCGGAGACATGGCCAACATCGCGGTGCAGCGAATCAAGCGGGAGTTCAAGGAGGTGCTG
AAGAGCGAGGAGGCCCTCAACCAAGGGCAACAGCGCTACTTTTACAGCATTATGAGGATTTACAACTCCAGGCCCCAGTGGGAGGCCCTG
CAGACCCGCTACATTCACAGCCTTCAGCACCAACAGCTGCTTGGCTATATTACTCAACGGGAAGCCTTGTCTTATGCTCTTGTACTTAGA
GATTCAACCAAGAGAGCCTCAGCCAAGGTAGCTCCTCAAAGAACCATTCCCCGGAAAACTTCAGCCATGACAAGAAGATGTCCATCAGTA
CTACCTGTATCTGTGGTTCTACCTAGGGCCCAAAGTAAAAGGCGCCAAGTGCTCAGGAACTGAGACTTGGCAGCATTTTCAGAAACAGGA
AGTTTGCCCATGTATCCCTGGTATCTAGTGGGTGCTTTGTGCATATTTGTTGAATGACAGTGAATAATTTCTAATATAAACCCCAGACCT
AAAAATAATCTCTGATTCATATAAATTTTGCCAGCAAGACCAAGAGGTTTAAGAGCAATGGAGCCGAGAGTAAGCAAGCCTTTCCACATA
ACATCCGTAGAGTAAAAGGACTTTTAAAGCTTTCAAAGTAAAAGTTTACTTTGGATTAGAACCTCCTAGAGAAGTCCCCAAAACAGTGAA
AGGGGACAAGTGACCAGAAAGGGCCAGGTCCTCTGTCACTGGGTGACCATTTCTAGCAAACTATCATGTATACATTTTGAAAATATACAA
AACAACACAGGATTAACACTGATCATAGAGGGCTTACAAAATCATATCATCCCAGCAACAATTGGCCTGTGCATTTCCTTCCTCTCCTCT
TCCCCATCAGGCAATGGCATTTAATTTAGCCCCAAAGCTCTTCTAGTTCTCACCTGGCTTGATCACTTTGTGTCATCTTGAAACATGCAC
TTATCAGTCTACCACAGCTGAATTGATTTTATGGAGCTGTAACTGGCCTTGACATGGGTAAGAGAATTCATGCACACATCTTTCCCTGGC
AGCTGAAGAAACACTCTTAAAAGAATATGAAATAACCCCGAAGACTTTATTTCATGTCCAACCTTTCTGTGGCCTCAAATGTTTTAAGCA
AAGCACATAAAACTATACTAAGCAGTGAGTCAGTTCATCATACAAAGGCATAGTATTGCATTAGTTATATTTAATGGTTATTTTCATCAC
CAGAGATTTTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTTAAAAATCAGTCACCAGTGCTCATGTAAAGTCAAAACAAAAAAATGCT
TCTTCCTCATCATTGTAAAATACGTCACCCCATTCAAATCAGCTGATGCCTATGAAAGGAATTGGTTGAGGTGGAATATAAATTTTATTG
ACAAGGGATGTGCTAACATTGATCTGAAAGGAATATGCTGCATTATTATCAGAAAAAAAATCATTATCTTCTCTTTGCAATCATGAACAT
ATAAGATATAAAAATTGTCATGAGATTCTGAACACTAGTATTGAGGTCCAGCACTACTTCCAAGCTCCTATCTGACTTTTCTTTGGGAGG
AATGGGAAGTGTGGTTGGCCCACTAGTCTGATAAGAAGTCTTGGTGAAGCTAATACTGACCTTGCTGGGGTCATTCACTTAATCATAAAG
AAGCTTCTTGACCTCTGCAAATTCTGGCCCTGACATGTATTCTAAATACCTTTACAGTTCTAAGTGAGAAATTGTTACATAGGCTTAGAA
GAAGAAAAGACTCCAGTGGGACTGGGATGAGCTGCGTGGTAGCCCTCATAAAGCAGAGAGGTTGGTGGGAGACCTAACTTGGCCTATACT
AGCCTTCCCATTGTTTTCACAATGGGCCTTTTATCCCCTCTGAACTGCTTGCCTGCACTTTTATCTTAGACTTTTTTTTTTGATGAGCAT
AGGTATTTGCTGTGTAAGCCTTATCTAGTGATGCAGAATATCAAGGAGGCCACTTACAATTCTAAGCTGCATCCCTGTATCAAAAATCTC
AGAAGCACCTTGCATCTGATAGAGGCTTGTAAGAGCAGATTGACAGGTGAATGTCACAGGTCCATAAGAAGGACAAATAGATCTAAGTTG
GGCAAAAGATTCTATTTGTTGGTTCTCCCTTCTAAGATGATAATATGCCCCTGCACTTGAGACCTTACTTGAAACTTAAAGAAATGTGAT
TGGTTATGGTGCTGGGAGAAAGGGGTGCACCTGGGAGGACACTGAGAAAGATAAACAAAGGGGAAGGAAGAGAGGACAACCTGTAACTTT
TAGAAAAGGCTACTACTTTTAACTGTTGGAGAAAATGCTATTGCTTGCACTCACAATGTCAATATACATTATCAGAACTTAAGTCCTGGA
GATCAGAGACTGTTTCACCTGAAATTGCTAGCACCTAGAATGGCCCCCGGTTGCGGTAGGCAATCAATAATACTCTTTGAATAATAATGA
GGTTTTCAAAGATTCTGCAAGTCAGACTGGCCTCTGGCTTTGTGATATTATTCTAGAGTCCCAGTAACCCACAATTGTTGATGAGGAGTA
GCAGTAGAATATCAAGGACAGGTGATGAGACAACAAGTCTATCCCGCAAAGGGCAGGGAATAGAGGGTACACTGGAAAGAATTTGAAAAC
AATAATAAAGCTGACTACAAGTTGGTCTATTTTTTATTACCACCATGAGCCAGCAATTCTAAATAATATCAGTGATAAAGTATTCATCCC
CCAAAATATTTTATTGAACTAGGTTCTAAACAATTTCTGAGGTTACTGTTGAGTTTTAAATAATACATATATGTAAGCTTCAAAGTAGCA
CATTTGTACTGTTTTTCTTCAATAATCATTTTACTCCACATGGAAACTAATTCAGGGAATATCCTGCTATATAGTTGGCTCTCAGTACAT
ATGGTGTCAGCTCATGTGTCTCCAGCCCCTGTAGTACTATATATTATTGCATTTAAATGATGGATTTGAAATAAACAATGATTATACCAT
GATTGTAAAGACAAGAAACAGAACCTGAAATACTTCAATTTTTGATTCTATGTGGCAGTTTAGAGTTTTTTAAAACAATTTTTATGTATA

>In-frame_ENST00000295963_ENST00000313851_TCGA-86-A4P7-01A_UBE2K_chr4_39700010_+_FAM216B_chr13_43360899_length(amino acids)=127AA_start in transcript=219_stop in transcript=602
MANIAVQRIKREFKEVLKSEEALNQGQQRYFYSIMRIYNSRPQWEALQTRYIHSLQHQQLLGYITQREALSYALVLRDSTKRASAKVAPQ

--------------------------------------------------------------
>In-frame_ENST00000503368_ENST00000537894_TCGA-86-A4P7-01A_UBE2K_chr4_39700010_+_FAM216B_chr13_43360899_length(transcript)=3306nt_BP=228nt
GATTCCACACTGAGGCGAGCGCGGCGGCCGGGGTGGTAGTGGCAGTGTTCGTGTGCTCAGGTCTGAATCGCCGAGGGAGGAGGCGGTGGA
GGAAGAGGTGGCGGCGGTGGCGGTGGTCGTAGCGGTGGCGGAGGAGGCGGGTACGAATCAGCTGCGGGCGGAGACATGGCCAACATCGCG
GTGCAGCGAATCAAGCGGGAGTTCAAGGAGGTGCTGAAGAGCGAGGAGGCCCTCAACCAAGGGCAACAGCGCTACTTTTACAGCATTATG
AGGATTTACAACTCCAGGCCCCAGTGGGAGGCCCTGCAGACCCGCTACATTCACAGCCTTCAGCACCAACAGCTGCTTGGCTATATTACT
CAACGGGAAGCCTTGTCTTATGCTCTTGTACTTAGAGATTCAACCAAGAGAGCCTCAGCCAAGGTAGCTCCTCAAAGAACCATTCCCCGG
AAAACTTCAGCCATGACAAGAAGATGTCCATCAGTACTACCTGTATCTGTGGTTCTACCTAGGGCCCAAAGTAAAAGGCGCCAAGTGCTC
AGGAACTGAGACTTGGCAGCATTTTCAGAAACAGGAAGTTTGCCCATGTATCCCTGGTATCTAGTGGGTGCTTTGTGCATATTTGTTGAA
TGACAGTGAATAATTTCTAATATAAACCCCAGACCTAAAAATAATCTCTGATTCATATAAATTTTGCCAGCAAGACCAAGAGGTTTAAGA
GCAATGGAGCCGAGAGTAAGCAAGCCTTTCCACATAACATCCGTAGAGTAAAAGGACTTTTAAAGCTTTCAAAGTAAAAGTTTACTTTGG
ATTAGAACCTCCTAGAGAAGTCCCCAAAACAGTGAAAGGGGACAAGTGACCAGAAAGGGCCAGGTCCTCTGTCACTGGGTGACCATTTCT
AGCAAACTATCATGTATACATTTTGAAAATATACAAAACAACACAGGATTAACACTGATCATAGAGGGCTTACAAAATCATATCATCCCA
GCAACAATTGGCCTGTGCATTTCCTTCCTCTCCTCTTCCCCATCAGGCAATGGCATTTAATTTAGCCCCAAAGCTCTTCTAGTTCTCACC
TGGCTTGATCACTTTGTGTCATCTTGAAACATGCACTTATCAGTCTACCACAGCTGAATTGATTTTATGGAGCTGTAACTGGCCTTGACA
TGGGTAAGAGAATTCATGCACACATCTTTCCCTGGCAGCTGAAGAAACACTCTTAAAAGAATATGAAATAACCCCGAAGACTTTATTTCA
TGTCCAACCTTTCTGTGGCCTCAAATGTTTTAAGCAAAGCACATAAAACTATACTAAGCAGTGAGTCAGTTCATCATACAAAGGCATAGT
ATTGCATTAGTTATATTTAATGGTTATTTTCATCACCAGAGATTTTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTTAAAAATCAGTC
ACCAGTGCTCATGTAAAGTCAAAACAAAAAAATGCTTCTTCCTCATCATTGTAAAATACGTCACCCCATTCAAATCAGCTGATGCCTATG
AAAGGAATTGGTTGAGGTGGAATATAAATTTTATTGACAAGGGATGTGCTAACATTGATCTGAAAGGAATATGCTGCATTATTATCAGAA
AAAAAATCATTATCTTCTCTTTGCAATCATGAACATATAAGATATAAAAATTGTCATGAGATTCTGAACACTAGTATTGAGGTCCAGCAC
TACTTCCAAGCTCCTATCTGACTTTTCTTTGGGAGGAATGGGAAGTGTGGTTGGCCCACTAGTCTGATAAGAAGTCTTGGTGAAGCTAAT
ACTGACCTTGCTGGGGTCATTCACTTAATCATAAAGAAGCTTCTTGACCTCTGCAAATTCTGGCCCTGACATGTATTCTAAATACCTTTA
CAGTTCTAAGTGAGAAATTGTTACATAGGCTTAGAAGAAGAAAAGACTCCAGTGGGACTGGGATGAGCTGCGTGGTAGCCCTCATAAAGC
AGAGAGGTTGGTGGGAGACCTAACTTGGCCTATACTAGCCTTCCCATTGTTTTCACAATGGGCCTTTTATCCCCTCTGAACTGCTTGCCT
GCACTTTTATCTTAGACTTTTTTTTTTGATGAGCATAGGTATTTGCTGTGTAAGCCTTATCTAGTGATGCAGAATATCAAGGAGGCCACT
TACAATTCTAAGCTGCATCCCTGTATCAAAAATCTCAGAAGCACCTTGCATCTGATAGAGGCTTGTAAGAGCAGATTGACAGGTGAATGT
CACAGGTCCATAAGAAGGACAAATAGATCTAAGTTGGGCAAAAGATTCTATTTGTTGGTTCTCCCTTCTAAGATGATAATATGCCCCTGC
ACTTGAGACCTTACTTGAAACTTAAAGAAATGTGATTGGTTATGGTGCTGGGAGAAAGGGGTGCACCTGGGAGGACACTGAGAAAGATAA
ACAAAGGGGAAGGAAGAGAGGACAACCTGTAACTTTTAGAAAAGGCTACTACTTTTAACTGTTGGAGAAAATGCTATTGCTTGCACTCAC
AATGTCAATATACATTATCAGAACTTAAGTCCTGGAGATCAGAGACTGTTTCACCTGAAATTGCTAGCACCTAGAATGGCCCCCGGTTGC
GGTAGGCAATCAATAATACTCTTTGAATAATAATGAGGTTTTCAAAGATTCTGCAAGTCAGACTGGCCTCTGGCTTTGTGATATTATTCT
AGAGTCCCAGTAACCCACAATTGTTGATGAGGAGTAGCAGTAGAATATCAAGGACAGGTGATGAGACAACAAGTCTATCCCGCAAAGGGC
AGGGAATAGAGGGTACACTGGAAAGAATTTGAAAACAATAATAAAGCTGACTACAAGTTGGTCTATTTTTTATTACCACCATGAGCCAGC
AATTCTAAATAATATCAGTGATAAAGTATTCATCCCCCAAAATATTTTATTGAACTAGGTTCTAAACAATTTCTGAGGTTACTGTTGAGT
TTTAAATAATACATATATGTAAGCTTCAAAGTAGCACATTTGTACTGTTTTTCTTCAATAATCATTTTACTCCACATGGAAACTAATTCA
GGGAATATCCTGCTATATAGTTGGCTCTCAGTACATATGGTGTCAGCTCATGTGTCTCCAGCCCCTGTAGTACTATATATTATTGCATTT
AAATGATGGATTTGAAATAAACAATGATTATACCATGATTGTAAAGACAAGAAACAGAACCTGAAATACTTCAATTTTTGATTCTATGTG

>In-frame_ENST00000503368_ENST00000537894_TCGA-86-A4P7-01A_UBE2K_chr4_39700010_+_FAM216B_chr13_43360899_length(amino acids)=127AA_start in transcript=165_stop in transcript=548
MANIAVQRIKREFKEVLKSEEALNQGQQRYFYSIMRIYNSRPQWEALQTRYIHSLQHQQLLGYITQREALSYALVLRDSTKRASAKVAPQ

--------------------------------------------------------------
>In-frame_ENST00000503368_ENST00000313851_TCGA-86-A4P7-01A_UBE2K_chr4_39700010_+_FAM216B_chr13_43360899_length(transcript)=3306nt_BP=228nt
GATTCCACACTGAGGCGAGCGCGGCGGCCGGGGTGGTAGTGGCAGTGTTCGTGTGCTCAGGTCTGAATCGCCGAGGGAGGAGGCGGTGGA
GGAAGAGGTGGCGGCGGTGGCGGTGGTCGTAGCGGTGGCGGAGGAGGCGGGTACGAATCAGCTGCGGGCGGAGACATGGCCAACATCGCG
GTGCAGCGAATCAAGCGGGAGTTCAAGGAGGTGCTGAAGAGCGAGGAGGCCCTCAACCAAGGGCAACAGCGCTACTTTTACAGCATTATG
AGGATTTACAACTCCAGGCCCCAGTGGGAGGCCCTGCAGACCCGCTACATTCACAGCCTTCAGCACCAACAGCTGCTTGGCTATATTACT
CAACGGGAAGCCTTGTCTTATGCTCTTGTACTTAGAGATTCAACCAAGAGAGCCTCAGCCAAGGTAGCTCCTCAAAGAACCATTCCCCGG
AAAACTTCAGCCATGACAAGAAGATGTCCATCAGTACTACCTGTATCTGTGGTTCTACCTAGGGCCCAAAGTAAAAGGCGCCAAGTGCTC
AGGAACTGAGACTTGGCAGCATTTTCAGAAACAGGAAGTTTGCCCATGTATCCCTGGTATCTAGTGGGTGCTTTGTGCATATTTGTTGAA
TGACAGTGAATAATTTCTAATATAAACCCCAGACCTAAAAATAATCTCTGATTCATATAAATTTTGCCAGCAAGACCAAGAGGTTTAAGA
GCAATGGAGCCGAGAGTAAGCAAGCCTTTCCACATAACATCCGTAGAGTAAAAGGACTTTTAAAGCTTTCAAAGTAAAAGTTTACTTTGG
ATTAGAACCTCCTAGAGAAGTCCCCAAAACAGTGAAAGGGGACAAGTGACCAGAAAGGGCCAGGTCCTCTGTCACTGGGTGACCATTTCT
AGCAAACTATCATGTATACATTTTGAAAATATACAAAACAACACAGGATTAACACTGATCATAGAGGGCTTACAAAATCATATCATCCCA
GCAACAATTGGCCTGTGCATTTCCTTCCTCTCCTCTTCCCCATCAGGCAATGGCATTTAATTTAGCCCCAAAGCTCTTCTAGTTCTCACC
TGGCTTGATCACTTTGTGTCATCTTGAAACATGCACTTATCAGTCTACCACAGCTGAATTGATTTTATGGAGCTGTAACTGGCCTTGACA
TGGGTAAGAGAATTCATGCACACATCTTTCCCTGGCAGCTGAAGAAACACTCTTAAAAGAATATGAAATAACCCCGAAGACTTTATTTCA
TGTCCAACCTTTCTGTGGCCTCAAATGTTTTAAGCAAAGCACATAAAACTATACTAAGCAGTGAGTCAGTTCATCATACAAAGGCATAGT
ATTGCATTAGTTATATTTAATGGTTATTTTCATCACCAGAGATTTTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTTAAAAATCAGTC
ACCAGTGCTCATGTAAAGTCAAAACAAAAAAATGCTTCTTCCTCATCATTGTAAAATACGTCACCCCATTCAAATCAGCTGATGCCTATG
AAAGGAATTGGTTGAGGTGGAATATAAATTTTATTGACAAGGGATGTGCTAACATTGATCTGAAAGGAATATGCTGCATTATTATCAGAA
AAAAAATCATTATCTTCTCTTTGCAATCATGAACATATAAGATATAAAAATTGTCATGAGATTCTGAACACTAGTATTGAGGTCCAGCAC
TACTTCCAAGCTCCTATCTGACTTTTCTTTGGGAGGAATGGGAAGTGTGGTTGGCCCACTAGTCTGATAAGAAGTCTTGGTGAAGCTAAT
ACTGACCTTGCTGGGGTCATTCACTTAATCATAAAGAAGCTTCTTGACCTCTGCAAATTCTGGCCCTGACATGTATTCTAAATACCTTTA
CAGTTCTAAGTGAGAAATTGTTACATAGGCTTAGAAGAAGAAAAGACTCCAGTGGGACTGGGATGAGCTGCGTGGTAGCCCTCATAAAGC
AGAGAGGTTGGTGGGAGACCTAACTTGGCCTATACTAGCCTTCCCATTGTTTTCACAATGGGCCTTTTATCCCCTCTGAACTGCTTGCCT
GCACTTTTATCTTAGACTTTTTTTTTTGATGAGCATAGGTATTTGCTGTGTAAGCCTTATCTAGTGATGCAGAATATCAAGGAGGCCACT
TACAATTCTAAGCTGCATCCCTGTATCAAAAATCTCAGAAGCACCTTGCATCTGATAGAGGCTTGTAAGAGCAGATTGACAGGTGAATGT
CACAGGTCCATAAGAAGGACAAATAGATCTAAGTTGGGCAAAAGATTCTATTTGTTGGTTCTCCCTTCTAAGATGATAATATGCCCCTGC
ACTTGAGACCTTACTTGAAACTTAAAGAAATGTGATTGGTTATGGTGCTGGGAGAAAGGGGTGCACCTGGGAGGACACTGAGAAAGATAA
ACAAAGGGGAAGGAAGAGAGGACAACCTGTAACTTTTAGAAAAGGCTACTACTTTTAACTGTTGGAGAAAATGCTATTGCTTGCACTCAC
AATGTCAATATACATTATCAGAACTTAAGTCCTGGAGATCAGAGACTGTTTCACCTGAAATTGCTAGCACCTAGAATGGCCCCCGGTTGC
GGTAGGCAATCAATAATACTCTTTGAATAATAATGAGGTTTTCAAAGATTCTGCAAGTCAGACTGGCCTCTGGCTTTGTGATATTATTCT
AGAGTCCCAGTAACCCACAATTGTTGATGAGGAGTAGCAGTAGAATATCAAGGACAGGTGATGAGACAACAAGTCTATCCCGCAAAGGGC
AGGGAATAGAGGGTACACTGGAAAGAATTTGAAAACAATAATAAAGCTGACTACAAGTTGGTCTATTTTTTATTACCACCATGAGCCAGC
AATTCTAAATAATATCAGTGATAAAGTATTCATCCCCCAAAATATTTTATTGAACTAGGTTCTAAACAATTTCTGAGGTTACTGTTGAGT
TTTAAATAATACATATATGTAAGCTTCAAAGTAGCACATTTGTACTGTTTTTCTTCAATAATCATTTTACTCCACATGGAAACTAATTCA
GGGAATATCCTGCTATATAGTTGGCTCTCAGTACATATGGTGTCAGCTCATGTGTCTCCAGCCCCTGTAGTACTATATATTATTGCATTT
AAATGATGGATTTGAAATAAACAATGATTATACCATGATTGTAAAGACAAGAAACAGAACCTGAAATACTTCAATTTTTGATTCTATGTG

>In-frame_ENST00000503368_ENST00000313851_TCGA-86-A4P7-01A_UBE2K_chr4_39700010_+_FAM216B_chr13_43360899_length(amino acids)=127AA_start in transcript=165_stop in transcript=548
MANIAVQRIKREFKEVLKSEEALNQGQQRYFYSIMRIYNSRPQWEALQTRYIHSLQHQQLLGYITQREALSYALVLRDSTKRASAKVAPQ

--------------------------------------------------------------
>In-frame_ENST00000445950_ENST00000537894_TCGA-86-A4P7-01A_UBE2K_chr4_39700010_+_FAM216B_chr13_43360899_length(transcript)=3293nt_BP=215nt
GGCGAGCGCGGCGGCCGGGGTGGTAGTGGCAGTGTTCGTGTGCTCAGGTCTGAATCGCCGAGGGAGGAGGCGGTGGAGGAAGAGGTGGCG
GCGGTGGCGGTGGTCGTAGCGGTGGCGGAGGAGGCGGGTACGAATCAGCTGCGGGCGGAGACATGGCCAACATCGCGGTGCAGCGAATCA
AGCGGGAGTTCAAGGAGGTGCTGAAGAGCGAGGAGGCCCTCAACCAAGGGCAACAGCGCTACTTTTACAGCATTATGAGGATTTACAACT
CCAGGCCCCAGTGGGAGGCCCTGCAGACCCGCTACATTCACAGCCTTCAGCACCAACAGCTGCTTGGCTATATTACTCAACGGGAAGCCT
TGTCTTATGCTCTTGTACTTAGAGATTCAACCAAGAGAGCCTCAGCCAAGGTAGCTCCTCAAAGAACCATTCCCCGGAAAACTTCAGCCA
TGACAAGAAGATGTCCATCAGTACTACCTGTATCTGTGGTTCTACCTAGGGCCCAAAGTAAAAGGCGCCAAGTGCTCAGGAACTGAGACT
TGGCAGCATTTTCAGAAACAGGAAGTTTGCCCATGTATCCCTGGTATCTAGTGGGTGCTTTGTGCATATTTGTTGAATGACAGTGAATAA
TTTCTAATATAAACCCCAGACCTAAAAATAATCTCTGATTCATATAAATTTTGCCAGCAAGACCAAGAGGTTTAAGAGCAATGGAGCCGA
GAGTAAGCAAGCCTTTCCACATAACATCCGTAGAGTAAAAGGACTTTTAAAGCTTTCAAAGTAAAAGTTTACTTTGGATTAGAACCTCCT
AGAGAAGTCCCCAAAACAGTGAAAGGGGACAAGTGACCAGAAAGGGCCAGGTCCTCTGTCACTGGGTGACCATTTCTAGCAAACTATCAT
GTATACATTTTGAAAATATACAAAACAACACAGGATTAACACTGATCATAGAGGGCTTACAAAATCATATCATCCCAGCAACAATTGGCC
TGTGCATTTCCTTCCTCTCCTCTTCCCCATCAGGCAATGGCATTTAATTTAGCCCCAAAGCTCTTCTAGTTCTCACCTGGCTTGATCACT
TTGTGTCATCTTGAAACATGCACTTATCAGTCTACCACAGCTGAATTGATTTTATGGAGCTGTAACTGGCCTTGACATGGGTAAGAGAAT
TCATGCACACATCTTTCCCTGGCAGCTGAAGAAACACTCTTAAAAGAATATGAAATAACCCCGAAGACTTTATTTCATGTCCAACCTTTC
TGTGGCCTCAAATGTTTTAAGCAAAGCACATAAAACTATACTAAGCAGTGAGTCAGTTCATCATACAAAGGCATAGTATTGCATTAGTTA
TATTTAATGGTTATTTTCATCACCAGAGATTTTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTTAAAAATCAGTCACCAGTGCTCATG
TAAAGTCAAAACAAAAAAATGCTTCTTCCTCATCATTGTAAAATACGTCACCCCATTCAAATCAGCTGATGCCTATGAAAGGAATTGGTT
GAGGTGGAATATAAATTTTATTGACAAGGGATGTGCTAACATTGATCTGAAAGGAATATGCTGCATTATTATCAGAAAAAAAATCATTAT
CTTCTCTTTGCAATCATGAACATATAAGATATAAAAATTGTCATGAGATTCTGAACACTAGTATTGAGGTCCAGCACTACTTCCAAGCTC
CTATCTGACTTTTCTTTGGGAGGAATGGGAAGTGTGGTTGGCCCACTAGTCTGATAAGAAGTCTTGGTGAAGCTAATACTGACCTTGCTG
GGGTCATTCACTTAATCATAAAGAAGCTTCTTGACCTCTGCAAATTCTGGCCCTGACATGTATTCTAAATACCTTTACAGTTCTAAGTGA
GAAATTGTTACATAGGCTTAGAAGAAGAAAAGACTCCAGTGGGACTGGGATGAGCTGCGTGGTAGCCCTCATAAAGCAGAGAGGTTGGTG
GGAGACCTAACTTGGCCTATACTAGCCTTCCCATTGTTTTCACAATGGGCCTTTTATCCCCTCTGAACTGCTTGCCTGCACTTTTATCTT
AGACTTTTTTTTTTGATGAGCATAGGTATTTGCTGTGTAAGCCTTATCTAGTGATGCAGAATATCAAGGAGGCCACTTACAATTCTAAGC
TGCATCCCTGTATCAAAAATCTCAGAAGCACCTTGCATCTGATAGAGGCTTGTAAGAGCAGATTGACAGGTGAATGTCACAGGTCCATAA
GAAGGACAAATAGATCTAAGTTGGGCAAAAGATTCTATTTGTTGGTTCTCCCTTCTAAGATGATAATATGCCCCTGCACTTGAGACCTTA
CTTGAAACTTAAAGAAATGTGATTGGTTATGGTGCTGGGAGAAAGGGGTGCACCTGGGAGGACACTGAGAAAGATAAACAAAGGGGAAGG
AAGAGAGGACAACCTGTAACTTTTAGAAAAGGCTACTACTTTTAACTGTTGGAGAAAATGCTATTGCTTGCACTCACAATGTCAATATAC
ATTATCAGAACTTAAGTCCTGGAGATCAGAGACTGTTTCACCTGAAATTGCTAGCACCTAGAATGGCCCCCGGTTGCGGTAGGCAATCAA
TAATACTCTTTGAATAATAATGAGGTTTTCAAAGATTCTGCAAGTCAGACTGGCCTCTGGCTTTGTGATATTATTCTAGAGTCCCAGTAA
CCCACAATTGTTGATGAGGAGTAGCAGTAGAATATCAAGGACAGGTGATGAGACAACAAGTCTATCCCGCAAAGGGCAGGGAATAGAGGG
TACACTGGAAAGAATTTGAAAACAATAATAAAGCTGACTACAAGTTGGTCTATTTTTTATTACCACCATGAGCCAGCAATTCTAAATAAT
ATCAGTGATAAAGTATTCATCCCCCAAAATATTTTATTGAACTAGGTTCTAAACAATTTCTGAGGTTACTGTTGAGTTTTAAATAATACA
TATATGTAAGCTTCAAAGTAGCACATTTGTACTGTTTTTCTTCAATAATCATTTTACTCCACATGGAAACTAATTCAGGGAATATCCTGC
TATATAGTTGGCTCTCAGTACATATGGTGTCAGCTCATGTGTCTCCAGCCCCTGTAGTACTATATATTATTGCATTTAAATGATGGATTT
GAAATAAACAATGATTATACCATGATTGTAAAGACAAGAAACAGAACCTGAAATACTTCAATTTTTGATTCTATGTGGCAGTTTAGAGTT

>In-frame_ENST00000445950_ENST00000537894_TCGA-86-A4P7-01A_UBE2K_chr4_39700010_+_FAM216B_chr13_43360899_length(amino acids)=127AA_start in transcript=152_stop in transcript=535
MANIAVQRIKREFKEVLKSEEALNQGQQRYFYSIMRIYNSRPQWEALQTRYIHSLQHQQLLGYITQREALSYALVLRDSTKRASAKVAPQ

--------------------------------------------------------------
>In-frame_ENST00000445950_ENST00000313851_TCGA-86-A4P7-01A_UBE2K_chr4_39700010_+_FAM216B_chr13_43360899_length(transcript)=3293nt_BP=215nt
GGCGAGCGCGGCGGCCGGGGTGGTAGTGGCAGTGTTCGTGTGCTCAGGTCTGAATCGCCGAGGGAGGAGGCGGTGGAGGAAGAGGTGGCG
GCGGTGGCGGTGGTCGTAGCGGTGGCGGAGGAGGCGGGTACGAATCAGCTGCGGGCGGAGACATGGCCAACATCGCGGTGCAGCGAATCA
AGCGGGAGTTCAAGGAGGTGCTGAAGAGCGAGGAGGCCCTCAACCAAGGGCAACAGCGCTACTTTTACAGCATTATGAGGATTTACAACT
CCAGGCCCCAGTGGGAGGCCCTGCAGACCCGCTACATTCACAGCCTTCAGCACCAACAGCTGCTTGGCTATATTACTCAACGGGAAGCCT
TGTCTTATGCTCTTGTACTTAGAGATTCAACCAAGAGAGCCTCAGCCAAGGTAGCTCCTCAAAGAACCATTCCCCGGAAAACTTCAGCCA
TGACAAGAAGATGTCCATCAGTACTACCTGTATCTGTGGTTCTACCTAGGGCCCAAAGTAAAAGGCGCCAAGTGCTCAGGAACTGAGACT
TGGCAGCATTTTCAGAAACAGGAAGTTTGCCCATGTATCCCTGGTATCTAGTGGGTGCTTTGTGCATATTTGTTGAATGACAGTGAATAA
TTTCTAATATAAACCCCAGACCTAAAAATAATCTCTGATTCATATAAATTTTGCCAGCAAGACCAAGAGGTTTAAGAGCAATGGAGCCGA
GAGTAAGCAAGCCTTTCCACATAACATCCGTAGAGTAAAAGGACTTTTAAAGCTTTCAAAGTAAAAGTTTACTTTGGATTAGAACCTCCT
AGAGAAGTCCCCAAAACAGTGAAAGGGGACAAGTGACCAGAAAGGGCCAGGTCCTCTGTCACTGGGTGACCATTTCTAGCAAACTATCAT
GTATACATTTTGAAAATATACAAAACAACACAGGATTAACACTGATCATAGAGGGCTTACAAAATCATATCATCCCAGCAACAATTGGCC
TGTGCATTTCCTTCCTCTCCTCTTCCCCATCAGGCAATGGCATTTAATTTAGCCCCAAAGCTCTTCTAGTTCTCACCTGGCTTGATCACT
TTGTGTCATCTTGAAACATGCACTTATCAGTCTACCACAGCTGAATTGATTTTATGGAGCTGTAACTGGCCTTGACATGGGTAAGAGAAT
TCATGCACACATCTTTCCCTGGCAGCTGAAGAAACACTCTTAAAAGAATATGAAATAACCCCGAAGACTTTATTTCATGTCCAACCTTTC
TGTGGCCTCAAATGTTTTAAGCAAAGCACATAAAACTATACTAAGCAGTGAGTCAGTTCATCATACAAAGGCATAGTATTGCATTAGTTA
TATTTAATGGTTATTTTCATCACCAGAGATTTTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTTAAAAATCAGTCACCAGTGCTCATG
TAAAGTCAAAACAAAAAAATGCTTCTTCCTCATCATTGTAAAATACGTCACCCCATTCAAATCAGCTGATGCCTATGAAAGGAATTGGTT
GAGGTGGAATATAAATTTTATTGACAAGGGATGTGCTAACATTGATCTGAAAGGAATATGCTGCATTATTATCAGAAAAAAAATCATTAT
CTTCTCTTTGCAATCATGAACATATAAGATATAAAAATTGTCATGAGATTCTGAACACTAGTATTGAGGTCCAGCACTACTTCCAAGCTC
CTATCTGACTTTTCTTTGGGAGGAATGGGAAGTGTGGTTGGCCCACTAGTCTGATAAGAAGTCTTGGTGAAGCTAATACTGACCTTGCTG
GGGTCATTCACTTAATCATAAAGAAGCTTCTTGACCTCTGCAAATTCTGGCCCTGACATGTATTCTAAATACCTTTACAGTTCTAAGTGA
GAAATTGTTACATAGGCTTAGAAGAAGAAAAGACTCCAGTGGGACTGGGATGAGCTGCGTGGTAGCCCTCATAAAGCAGAGAGGTTGGTG
GGAGACCTAACTTGGCCTATACTAGCCTTCCCATTGTTTTCACAATGGGCCTTTTATCCCCTCTGAACTGCTTGCCTGCACTTTTATCTT
AGACTTTTTTTTTTGATGAGCATAGGTATTTGCTGTGTAAGCCTTATCTAGTGATGCAGAATATCAAGGAGGCCACTTACAATTCTAAGC
TGCATCCCTGTATCAAAAATCTCAGAAGCACCTTGCATCTGATAGAGGCTTGTAAGAGCAGATTGACAGGTGAATGTCACAGGTCCATAA
GAAGGACAAATAGATCTAAGTTGGGCAAAAGATTCTATTTGTTGGTTCTCCCTTCTAAGATGATAATATGCCCCTGCACTTGAGACCTTA
CTTGAAACTTAAAGAAATGTGATTGGTTATGGTGCTGGGAGAAAGGGGTGCACCTGGGAGGACACTGAGAAAGATAAACAAAGGGGAAGG
AAGAGAGGACAACCTGTAACTTTTAGAAAAGGCTACTACTTTTAACTGTTGGAGAAAATGCTATTGCTTGCACTCACAATGTCAATATAC
ATTATCAGAACTTAAGTCCTGGAGATCAGAGACTGTTTCACCTGAAATTGCTAGCACCTAGAATGGCCCCCGGTTGCGGTAGGCAATCAA
TAATACTCTTTGAATAATAATGAGGTTTTCAAAGATTCTGCAAGTCAGACTGGCCTCTGGCTTTGTGATATTATTCTAGAGTCCCAGTAA
CCCACAATTGTTGATGAGGAGTAGCAGTAGAATATCAAGGACAGGTGATGAGACAACAAGTCTATCCCGCAAAGGGCAGGGAATAGAGGG
TACACTGGAAAGAATTTGAAAACAATAATAAAGCTGACTACAAGTTGGTCTATTTTTTATTACCACCATGAGCCAGCAATTCTAAATAAT
ATCAGTGATAAAGTATTCATCCCCCAAAATATTTTATTGAACTAGGTTCTAAACAATTTCTGAGGTTACTGTTGAGTTTTAAATAATACA
TATATGTAAGCTTCAAAGTAGCACATTTGTACTGTTTTTCTTCAATAATCATTTTACTCCACATGGAAACTAATTCAGGGAATATCCTGC
TATATAGTTGGCTCTCAGTACATATGGTGTCAGCTCATGTGTCTCCAGCCCCTGTAGTACTATATATTATTGCATTTAAATGATGGATTT
GAAATAAACAATGATTATACCATGATTGTAAAGACAAGAAACAGAACCTGAAATACTTCAATTTTTGATTCTATGTGGCAGTTTAGAGTT

>In-frame_ENST00000445950_ENST00000313851_TCGA-86-A4P7-01A_UBE2K_chr4_39700010_+_FAM216B_chr13_43360899_length(amino acids)=127AA_start in transcript=152_stop in transcript=535
MANIAVQRIKREFKEVLKSEEALNQGQQRYFYSIMRIYNSRPQWEALQTRYIHSLQHQQLLGYITQREALSYALVLRDSTKRASAKVAPQ

--------------------------------------------------------------
>In-frame_ENST00000261427_ENST00000537894_TCGA-86-A4P7_UBE2K_chr4_39700010_+_FAM216B_chr13_43360898_length(transcript)=3425nt_BP=347nt
ACGCGAGCTCGTGGCTCGGGCGTATGGGGGCTCCAACGGGAGCGGCTGCGCCGCCCGGCCGGGTCAACGTCGTGCGCATGCGCCGACCCG
GCGCCATTTTGGTGGCCGGGCGCGGAGGTGATTCCACACTGAGGCGAGCGCGGCGGCCGGGGTGGTAGTGGCAGTGTTCGTGTGCTCAGG
TCTGAATCGCCGAGGGAGGAGGCGGTGGAGGAAGAGGTGGCGGCGGTGGCGGTGGTCGTAGCGGTGGCGGAGGAGGCGGGTACGAATCAG
CTGCGGGCGGAGACATGGCCAACATCGCGGTGCAGCGAATCAAGCGGGAGTTCAAGGAGGTGCTGAAGAGCGAGGAGGCCCTCAACCAAG
GGCAACAGCGCTACTTTTACAGCATTATGAGGATTTACAACTCCAGGCCCCAGTGGGAGGCCCTGCAGACCCGCTACATTCACAGCCTTC
AGCACCAACAGCTGCTTGGCTATATTACTCAACGGGAAGCCTTGTCTTATGCTCTTGTACTTAGAGATTCAACCAAGAGAGCCTCAGCCA
AGGTAGCTCCTCAAAGAACCATTCCCCGGAAAACTTCAGCCATGACAAGAAGATGTCCATCAGTACTACCTGTATCTGTGGTTCTACCTA
GGGCCCAAAGTAAAAGGCGCCAAGTGCTCAGGAACTGAGACTTGGCAGCATTTTCAGAAACAGGAAGTTTGCCCATGTATCCCTGGTATC
TAGTGGGTGCTTTGTGCATATTTGTTGAATGACAGTGAATAATTTCTAATATAAACCCCAGACCTAAAAATAATCTCTGATTCATATAAA
TTTTGCCAGCAAGACCAAGAGGTTTAAGAGCAATGGAGCCGAGAGTAAGCAAGCCTTTCCACATAACATCCGTAGAGTAAAAGGACTTTT
AAAGCTTTCAAAGTAAAAGTTTACTTTGGATTAGAACCTCCTAGAGAAGTCCCCAAAACAGTGAAAGGGGACAAGTGACCAGAAAGGGCC
AGGTCCTCTGTCACTGGGTGACCATTTCTAGCAAACTATCATGTATACATTTTGAAAATATACAAAACAACACAGGATTAACACTGATCA
TAGAGGGCTTACAAAATCATATCATCCCAGCAACAATTGGCCTGTGCATTTCCTTCCTCTCCTCTTCCCCATCAGGCAATGGCATTTAAT
TTAGCCCCAAAGCTCTTCTAGTTCTCACCTGGCTTGATCACTTTGTGTCATCTTGAAACATGCACTTATCAGTCTACCACAGCTGAATTG
ATTTTATGGAGCTGTAACTGGCCTTGACATGGGTAAGAGAATTCATGCACACATCTTTCCCTGGCAGCTGAAGAAACACTCTTAAAAGAA
TATGAAATAACCCCGAAGACTTTATTTCATGTCCAACCTTTCTGTGGCCTCAAATGTTTTAAGCAAAGCACATAAAACTATACTAAGCAG
TGAGTCAGTTCATCATACAAAGGCATAGTATTGCATTAGTTATATTTAATGGTTATTTTCATCACCAGAGATTTTGTGTGTGTGTGTGTG
TGTGTGTGTGTGTGTGTTAAAAATCAGTCACCAGTGCTCATGTAAAGTCAAAACAAAAAAATGCTTCTTCCTCATCATTGTAAAATACGT
CACCCCATTCAAATCAGCTGATGCCTATGAAAGGAATTGGTTGAGGTGGAATATAAATTTTATTGACAAGGGATGTGCTAACATTGATCT
GAAAGGAATATGCTGCATTATTATCAGAAAAAAAATCATTATCTTCTCTTTGCAATCATGAACATATAAGATATAAAAATTGTCATGAGA
TTCTGAACACTAGTATTGAGGTCCAGCACTACTTCCAAGCTCCTATCTGACTTTTCTTTGGGAGGAATGGGAAGTGTGGTTGGCCCACTA
GTCTGATAAGAAGTCTTGGTGAAGCTAATACTGACCTTGCTGGGGTCATTCACTTAATCATAAAGAAGCTTCTTGACCTCTGCAAATTCT
GGCCCTGACATGTATTCTAAATACCTTTACAGTTCTAAGTGAGAAATTGTTACATAGGCTTAGAAGAAGAAAAGACTCCAGTGGGACTGG
GATGAGCTGCGTGGTAGCCCTCATAAAGCAGAGAGGTTGGTGGGAGACCTAACTTGGCCTATACTAGCCTTCCCATTGTTTTCACAATGG
GCCTTTTATCCCCTCTGAACTGCTTGCCTGCACTTTTATCTTAGACTTTTTTTTTTGATGAGCATAGGTATTTGCTGTGTAAGCCTTATC
TAGTGATGCAGAATATCAAGGAGGCCACTTACAATTCTAAGCTGCATCCCTGTATCAAAAATCTCAGAAGCACCTTGCATCTGATAGAGG
CTTGTAAGAGCAGATTGACAGGTGAATGTCACAGGTCCATAAGAAGGACAAATAGATCTAAGTTGGGCAAAAGATTCTATTTGTTGGTTC
TCCCTTCTAAGATGATAATATGCCCCTGCACTTGAGACCTTACTTGAAACTTAAAGAAATGTGATTGGTTATGGTGCTGGGAGAAAGGGG
TGCACCTGGGAGGACACTGAGAAAGATAAACAAAGGGGAAGGAAGAGAGGACAACCTGTAACTTTTAGAAAAGGCTACTACTTTTAACTG
TTGGAGAAAATGCTATTGCTTGCACTCACAATGTCAATATACATTATCAGAACTTAAGTCCTGGAGATCAGAGACTGTTTCACCTGAAAT
TGCTAGCACCTAGAATGGCCCCCGGTTGCGGTAGGCAATCAATAATACTCTTTGAATAATAATGAGGTTTTCAAAGATTCTGCAAGTCAG
ACTGGCCTCTGGCTTTGTGATATTATTCTAGAGTCCCAGTAACCCACAATTGTTGATGAGGAGTAGCAGTAGAATATCAAGGACAGGTGA
TGAGACAACAAGTCTATCCCGCAAAGGGCAGGGAATAGAGGGTACACTGGAAAGAATTTGAAAACAATAATAAAGCTGACTACAAGTTGG
TCTATTTTTTATTACCACCATGAGCCAGCAATTCTAAATAATATCAGTGATAAAGTATTCATCCCCCAAAATATTTTATTGAACTAGGTT
CTAAACAATTTCTGAGGTTACTGTTGAGTTTTAAATAATACATATATGTAAGCTTCAAAGTAGCACATTTGTACTGTTTTTCTTCAATAA
TCATTTTACTCCACATGGAAACTAATTCAGGGAATATCCTGCTATATAGTTGGCTCTCAGTACATATGGTGTCAGCTCATGTGTCTCCAG
CCCCTGTAGTACTATATATTATTGCATTTAAATGATGGATTTGAAATAAACAATGATTATACCATGATTGTAAAGACAAGAAACAGAACC
TGAAATACTTCAATTTTTGATTCTATGTGGCAGTTTAGAGTTTTTTAAAACAATTTTTATGTATAATTTATATGCAATAAATGCCCTCAA

>In-frame_ENST00000261427_ENST00000537894_TCGA-86-A4P7_UBE2K_chr4_39700010_+_FAM216B_chr13_43360898_length(amino acids)=127AA_start in transcript=284_stop in transcript=667
MANIAVQRIKREFKEVLKSEEALNQGQQRYFYSIMRIYNSRPQWEALQTRYIHSLQHQQLLGYITQREALSYALVLRDSTKRASAKVAPQ

--------------------------------------------------------------
>In-frame_ENST00000261427_ENST00000313851_TCGA-86-A4P7_UBE2K_chr4_39700010_+_FAM216B_chr13_43360898_length(transcript)=3425nt_BP=347nt
ACGCGAGCTCGTGGCTCGGGCGTATGGGGGCTCCAACGGGAGCGGCTGCGCCGCCCGGCCGGGTCAACGTCGTGCGCATGCGCCGACCCG
GCGCCATTTTGGTGGCCGGGCGCGGAGGTGATTCCACACTGAGGCGAGCGCGGCGGCCGGGGTGGTAGTGGCAGTGTTCGTGTGCTCAGG
TCTGAATCGCCGAGGGAGGAGGCGGTGGAGGAAGAGGTGGCGGCGGTGGCGGTGGTCGTAGCGGTGGCGGAGGAGGCGGGTACGAATCAG
CTGCGGGCGGAGACATGGCCAACATCGCGGTGCAGCGAATCAAGCGGGAGTTCAAGGAGGTGCTGAAGAGCGAGGAGGCCCTCAACCAAG
GGCAACAGCGCTACTTTTACAGCATTATGAGGATTTACAACTCCAGGCCCCAGTGGGAGGCCCTGCAGACCCGCTACATTCACAGCCTTC
AGCACCAACAGCTGCTTGGCTATATTACTCAACGGGAAGCCTTGTCTTATGCTCTTGTACTTAGAGATTCAACCAAGAGAGCCTCAGCCA
AGGTAGCTCCTCAAAGAACCATTCCCCGGAAAACTTCAGCCATGACAAGAAGATGTCCATCAGTACTACCTGTATCTGTGGTTCTACCTA
GGGCCCAAAGTAAAAGGCGCCAAGTGCTCAGGAACTGAGACTTGGCAGCATTTTCAGAAACAGGAAGTTTGCCCATGTATCCCTGGTATC
TAGTGGGTGCTTTGTGCATATTTGTTGAATGACAGTGAATAATTTCTAATATAAACCCCAGACCTAAAAATAATCTCTGATTCATATAAA
TTTTGCCAGCAAGACCAAGAGGTTTAAGAGCAATGGAGCCGAGAGTAAGCAAGCCTTTCCACATAACATCCGTAGAGTAAAAGGACTTTT
AAAGCTTTCAAAGTAAAAGTTTACTTTGGATTAGAACCTCCTAGAGAAGTCCCCAAAACAGTGAAAGGGGACAAGTGACCAGAAAGGGCC
AGGTCCTCTGTCACTGGGTGACCATTTCTAGCAAACTATCATGTATACATTTTGAAAATATACAAAACAACACAGGATTAACACTGATCA
TAGAGGGCTTACAAAATCATATCATCCCAGCAACAATTGGCCTGTGCATTTCCTTCCTCTCCTCTTCCCCATCAGGCAATGGCATTTAAT
TTAGCCCCAAAGCTCTTCTAGTTCTCACCTGGCTTGATCACTTTGTGTCATCTTGAAACATGCACTTATCAGTCTACCACAGCTGAATTG
ATTTTATGGAGCTGTAACTGGCCTTGACATGGGTAAGAGAATTCATGCACACATCTTTCCCTGGCAGCTGAAGAAACACTCTTAAAAGAA
TATGAAATAACCCCGAAGACTTTATTTCATGTCCAACCTTTCTGTGGCCTCAAATGTTTTAAGCAAAGCACATAAAACTATACTAAGCAG
TGAGTCAGTTCATCATACAAAGGCATAGTATTGCATTAGTTATATTTAATGGTTATTTTCATCACCAGAGATTTTGTGTGTGTGTGTGTG
TGTGTGTGTGTGTGTGTTAAAAATCAGTCACCAGTGCTCATGTAAAGTCAAAACAAAAAAATGCTTCTTCCTCATCATTGTAAAATACGT
CACCCCATTCAAATCAGCTGATGCCTATGAAAGGAATTGGTTGAGGTGGAATATAAATTTTATTGACAAGGGATGTGCTAACATTGATCT
GAAAGGAATATGCTGCATTATTATCAGAAAAAAAATCATTATCTTCTCTTTGCAATCATGAACATATAAGATATAAAAATTGTCATGAGA
TTCTGAACACTAGTATTGAGGTCCAGCACTACTTCCAAGCTCCTATCTGACTTTTCTTTGGGAGGAATGGGAAGTGTGGTTGGCCCACTA
GTCTGATAAGAAGTCTTGGTGAAGCTAATACTGACCTTGCTGGGGTCATTCACTTAATCATAAAGAAGCTTCTTGACCTCTGCAAATTCT
GGCCCTGACATGTATTCTAAATACCTTTACAGTTCTAAGTGAGAAATTGTTACATAGGCTTAGAAGAAGAAAAGACTCCAGTGGGACTGG
GATGAGCTGCGTGGTAGCCCTCATAAAGCAGAGAGGTTGGTGGGAGACCTAACTTGGCCTATACTAGCCTTCCCATTGTTTTCACAATGG
GCCTTTTATCCCCTCTGAACTGCTTGCCTGCACTTTTATCTTAGACTTTTTTTTTTGATGAGCATAGGTATTTGCTGTGTAAGCCTTATC
TAGTGATGCAGAATATCAAGGAGGCCACTTACAATTCTAAGCTGCATCCCTGTATCAAAAATCTCAGAAGCACCTTGCATCTGATAGAGG
CTTGTAAGAGCAGATTGACAGGTGAATGTCACAGGTCCATAAGAAGGACAAATAGATCTAAGTTGGGCAAAAGATTCTATTTGTTGGTTC
TCCCTTCTAAGATGATAATATGCCCCTGCACTTGAGACCTTACTTGAAACTTAAAGAAATGTGATTGGTTATGGTGCTGGGAGAAAGGGG
TGCACCTGGGAGGACACTGAGAAAGATAAACAAAGGGGAAGGAAGAGAGGACAACCTGTAACTTTTAGAAAAGGCTACTACTTTTAACTG
TTGGAGAAAATGCTATTGCTTGCACTCACAATGTCAATATACATTATCAGAACTTAAGTCCTGGAGATCAGAGACTGTTTCACCTGAAAT
TGCTAGCACCTAGAATGGCCCCCGGTTGCGGTAGGCAATCAATAATACTCTTTGAATAATAATGAGGTTTTCAAAGATTCTGCAAGTCAG
ACTGGCCTCTGGCTTTGTGATATTATTCTAGAGTCCCAGTAACCCACAATTGTTGATGAGGAGTAGCAGTAGAATATCAAGGACAGGTGA
TGAGACAACAAGTCTATCCCGCAAAGGGCAGGGAATAGAGGGTACACTGGAAAGAATTTGAAAACAATAATAAAGCTGACTACAAGTTGG
TCTATTTTTTATTACCACCATGAGCCAGCAATTCTAAATAATATCAGTGATAAAGTATTCATCCCCCAAAATATTTTATTGAACTAGGTT
CTAAACAATTTCTGAGGTTACTGTTGAGTTTTAAATAATACATATATGTAAGCTTCAAAGTAGCACATTTGTACTGTTTTTCTTCAATAA
TCATTTTACTCCACATGGAAACTAATTCAGGGAATATCCTGCTATATAGTTGGCTCTCAGTACATATGGTGTCAGCTCATGTGTCTCCAG
CCCCTGTAGTACTATATATTATTGCATTTAAATGATGGATTTGAAATAAACAATGATTATACCATGATTGTAAAGACAAGAAACAGAACC
TGAAATACTTCAATTTTTGATTCTATGTGGCAGTTTAGAGTTTTTTAAAACAATTTTTATGTATAATTTATATGCAATAAATGCCCTCAA

>In-frame_ENST00000261427_ENST00000313851_TCGA-86-A4P7_UBE2K_chr4_39700010_+_FAM216B_chr13_43360898_length(amino acids)=127AA_start in transcript=284_stop in transcript=667
MANIAVQRIKREFKEVLKSEEALNQGQQRYFYSIMRIYNSRPQWEALQTRYIHSLQHQQLLGYITQREALSYALVLRDSTKRASAKVAPQ

--------------------------------------------------------------
>In-frame_ENST00000295963_ENST00000537894_TCGA-86-A4P7_UBE2K_chr4_39700010_+_FAM216B_chr13_43360898_length(transcript)=3360nt_BP=282nt
AACGTCGTGCGCATGCGCCGACCCGGCGCCATTTTGGTGGCCGGGCGCGGAGGTGATTCCACACTGAGGCGAGCGCGGCGGCCGGGGTGG
TAGTGGCAGTGTTCGTGTGCTCAGGTCTGAATCGCCGAGGGAGGAGGCGGTGGAGGAAGAGGTGGCGGCGGTGGCGGTGGTCGTAGCGGT
GGCGGAGGAGGCGGGTACGAATCAGCTGCGGGCGGAGACATGGCCAACATCGCGGTGCAGCGAATCAAGCGGGAGTTCAAGGAGGTGCTG
AAGAGCGAGGAGGCCCTCAACCAAGGGCAACAGCGCTACTTTTACAGCATTATGAGGATTTACAACTCCAGGCCCCAGTGGGAGGCCCTG
CAGACCCGCTACATTCACAGCCTTCAGCACCAACAGCTGCTTGGCTATATTACTCAACGGGAAGCCTTGTCTTATGCTCTTGTACTTAGA
GATTCAACCAAGAGAGCCTCAGCCAAGGTAGCTCCTCAAAGAACCATTCCCCGGAAAACTTCAGCCATGACAAGAAGATGTCCATCAGTA
CTACCTGTATCTGTGGTTCTACCTAGGGCCCAAAGTAAAAGGCGCCAAGTGCTCAGGAACTGAGACTTGGCAGCATTTTCAGAAACAGGA
AGTTTGCCCATGTATCCCTGGTATCTAGTGGGTGCTTTGTGCATATTTGTTGAATGACAGTGAATAATTTCTAATATAAACCCCAGACCT
AAAAATAATCTCTGATTCATATAAATTTTGCCAGCAAGACCAAGAGGTTTAAGAGCAATGGAGCCGAGAGTAAGCAAGCCTTTCCACATA
ACATCCGTAGAGTAAAAGGACTTTTAAAGCTTTCAAAGTAAAAGTTTACTTTGGATTAGAACCTCCTAGAGAAGTCCCCAAAACAGTGAA
AGGGGACAAGTGACCAGAAAGGGCCAGGTCCTCTGTCACTGGGTGACCATTTCTAGCAAACTATCATGTATACATTTTGAAAATATACAA
AACAACACAGGATTAACACTGATCATAGAGGGCTTACAAAATCATATCATCCCAGCAACAATTGGCCTGTGCATTTCCTTCCTCTCCTCT
TCCCCATCAGGCAATGGCATTTAATTTAGCCCCAAAGCTCTTCTAGTTCTCACCTGGCTTGATCACTTTGTGTCATCTTGAAACATGCAC
TTATCAGTCTACCACAGCTGAATTGATTTTATGGAGCTGTAACTGGCCTTGACATGGGTAAGAGAATTCATGCACACATCTTTCCCTGGC
AGCTGAAGAAACACTCTTAAAAGAATATGAAATAACCCCGAAGACTTTATTTCATGTCCAACCTTTCTGTGGCCTCAAATGTTTTAAGCA
AAGCACATAAAACTATACTAAGCAGTGAGTCAGTTCATCATACAAAGGCATAGTATTGCATTAGTTATATTTAATGGTTATTTTCATCAC
CAGAGATTTTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTTAAAAATCAGTCACCAGTGCTCATGTAAAGTCAAAACAAAAAAATGCT
TCTTCCTCATCATTGTAAAATACGTCACCCCATTCAAATCAGCTGATGCCTATGAAAGGAATTGGTTGAGGTGGAATATAAATTTTATTG
ACAAGGGATGTGCTAACATTGATCTGAAAGGAATATGCTGCATTATTATCAGAAAAAAAATCATTATCTTCTCTTTGCAATCATGAACAT
ATAAGATATAAAAATTGTCATGAGATTCTGAACACTAGTATTGAGGTCCAGCACTACTTCCAAGCTCCTATCTGACTTTTCTTTGGGAGG
AATGGGAAGTGTGGTTGGCCCACTAGTCTGATAAGAAGTCTTGGTGAAGCTAATACTGACCTTGCTGGGGTCATTCACTTAATCATAAAG
AAGCTTCTTGACCTCTGCAAATTCTGGCCCTGACATGTATTCTAAATACCTTTACAGTTCTAAGTGAGAAATTGTTACATAGGCTTAGAA
GAAGAAAAGACTCCAGTGGGACTGGGATGAGCTGCGTGGTAGCCCTCATAAAGCAGAGAGGTTGGTGGGAGACCTAACTTGGCCTATACT
AGCCTTCCCATTGTTTTCACAATGGGCCTTTTATCCCCTCTGAACTGCTTGCCTGCACTTTTATCTTAGACTTTTTTTTTTGATGAGCAT
AGGTATTTGCTGTGTAAGCCTTATCTAGTGATGCAGAATATCAAGGAGGCCACTTACAATTCTAAGCTGCATCCCTGTATCAAAAATCTC
AGAAGCACCTTGCATCTGATAGAGGCTTGTAAGAGCAGATTGACAGGTGAATGTCACAGGTCCATAAGAAGGACAAATAGATCTAAGTTG
GGCAAAAGATTCTATTTGTTGGTTCTCCCTTCTAAGATGATAATATGCCCCTGCACTTGAGACCTTACTTGAAACTTAAAGAAATGTGAT
TGGTTATGGTGCTGGGAGAAAGGGGTGCACCTGGGAGGACACTGAGAAAGATAAACAAAGGGGAAGGAAGAGAGGACAACCTGTAACTTT
TAGAAAAGGCTACTACTTTTAACTGTTGGAGAAAATGCTATTGCTTGCACTCACAATGTCAATATACATTATCAGAACTTAAGTCCTGGA
GATCAGAGACTGTTTCACCTGAAATTGCTAGCACCTAGAATGGCCCCCGGTTGCGGTAGGCAATCAATAATACTCTTTGAATAATAATGA
GGTTTTCAAAGATTCTGCAAGTCAGACTGGCCTCTGGCTTTGTGATATTATTCTAGAGTCCCAGTAACCCACAATTGTTGATGAGGAGTA
GCAGTAGAATATCAAGGACAGGTGATGAGACAACAAGTCTATCCCGCAAAGGGCAGGGAATAGAGGGTACACTGGAAAGAATTTGAAAAC
AATAATAAAGCTGACTACAAGTTGGTCTATTTTTTATTACCACCATGAGCCAGCAATTCTAAATAATATCAGTGATAAAGTATTCATCCC
CCAAAATATTTTATTGAACTAGGTTCTAAACAATTTCTGAGGTTACTGTTGAGTTTTAAATAATACATATATGTAAGCTTCAAAGTAGCA
CATTTGTACTGTTTTTCTTCAATAATCATTTTACTCCACATGGAAACTAATTCAGGGAATATCCTGCTATATAGTTGGCTCTCAGTACAT
ATGGTGTCAGCTCATGTGTCTCCAGCCCCTGTAGTACTATATATTATTGCATTTAAATGATGGATTTGAAATAAACAATGATTATACCAT
GATTGTAAAGACAAGAAACAGAACCTGAAATACTTCAATTTTTGATTCTATGTGGCAGTTTAGAGTTTTTTAAAACAATTTTTATGTATA

>In-frame_ENST00000295963_ENST00000537894_TCGA-86-A4P7_UBE2K_chr4_39700010_+_FAM216B_chr13_43360898_length(amino acids)=127AA_start in transcript=219_stop in transcript=602
MANIAVQRIKREFKEVLKSEEALNQGQQRYFYSIMRIYNSRPQWEALQTRYIHSLQHQQLLGYITQREALSYALVLRDSTKRASAKVAPQ

--------------------------------------------------------------
>In-frame_ENST00000295963_ENST00000313851_TCGA-86-A4P7_UBE2K_chr4_39700010_+_FAM216B_chr13_43360898_length(transcript)=3360nt_BP=282nt
AACGTCGTGCGCATGCGCCGACCCGGCGCCATTTTGGTGGCCGGGCGCGGAGGTGATTCCACACTGAGGCGAGCGCGGCGGCCGGGGTGG
TAGTGGCAGTGTTCGTGTGCTCAGGTCTGAATCGCCGAGGGAGGAGGCGGTGGAGGAAGAGGTGGCGGCGGTGGCGGTGGTCGTAGCGGT
GGCGGAGGAGGCGGGTACGAATCAGCTGCGGGCGGAGACATGGCCAACATCGCGGTGCAGCGAATCAAGCGGGAGTTCAAGGAGGTGCTG
AAGAGCGAGGAGGCCCTCAACCAAGGGCAACAGCGCTACTTTTACAGCATTATGAGGATTTACAACTCCAGGCCCCAGTGGGAGGCCCTG
CAGACCCGCTACATTCACAGCCTTCAGCACCAACAGCTGCTTGGCTATATTACTCAACGGGAAGCCTTGTCTTATGCTCTTGTACTTAGA
GATTCAACCAAGAGAGCCTCAGCCAAGGTAGCTCCTCAAAGAACCATTCCCCGGAAAACTTCAGCCATGACAAGAAGATGTCCATCAGTA
CTACCTGTATCTGTGGTTCTACCTAGGGCCCAAAGTAAAAGGCGCCAAGTGCTCAGGAACTGAGACTTGGCAGCATTTTCAGAAACAGGA
AGTTTGCCCATGTATCCCTGGTATCTAGTGGGTGCTTTGTGCATATTTGTTGAATGACAGTGAATAATTTCTAATATAAACCCCAGACCT
AAAAATAATCTCTGATTCATATAAATTTTGCCAGCAAGACCAAGAGGTTTAAGAGCAATGGAGCCGAGAGTAAGCAAGCCTTTCCACATA
ACATCCGTAGAGTAAAAGGACTTTTAAAGCTTTCAAAGTAAAAGTTTACTTTGGATTAGAACCTCCTAGAGAAGTCCCCAAAACAGTGAA
AGGGGACAAGTGACCAGAAAGGGCCAGGTCCTCTGTCACTGGGTGACCATTTCTAGCAAACTATCATGTATACATTTTGAAAATATACAA
AACAACACAGGATTAACACTGATCATAGAGGGCTTACAAAATCATATCATCCCAGCAACAATTGGCCTGTGCATTTCCTTCCTCTCCTCT
TCCCCATCAGGCAATGGCATTTAATTTAGCCCCAAAGCTCTTCTAGTTCTCACCTGGCTTGATCACTTTGTGTCATCTTGAAACATGCAC
TTATCAGTCTACCACAGCTGAATTGATTTTATGGAGCTGTAACTGGCCTTGACATGGGTAAGAGAATTCATGCACACATCTTTCCCTGGC
AGCTGAAGAAACACTCTTAAAAGAATATGAAATAACCCCGAAGACTTTATTTCATGTCCAACCTTTCTGTGGCCTCAAATGTTTTAAGCA
AAGCACATAAAACTATACTAAGCAGTGAGTCAGTTCATCATACAAAGGCATAGTATTGCATTAGTTATATTTAATGGTTATTTTCATCAC
CAGAGATTTTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTTAAAAATCAGTCACCAGTGCTCATGTAAAGTCAAAACAAAAAAATGCT
TCTTCCTCATCATTGTAAAATACGTCACCCCATTCAAATCAGCTGATGCCTATGAAAGGAATTGGTTGAGGTGGAATATAAATTTTATTG
ACAAGGGATGTGCTAACATTGATCTGAAAGGAATATGCTGCATTATTATCAGAAAAAAAATCATTATCTTCTCTTTGCAATCATGAACAT
ATAAGATATAAAAATTGTCATGAGATTCTGAACACTAGTATTGAGGTCCAGCACTACTTCCAAGCTCCTATCTGACTTTTCTTTGGGAGG
AATGGGAAGTGTGGTTGGCCCACTAGTCTGATAAGAAGTCTTGGTGAAGCTAATACTGACCTTGCTGGGGTCATTCACTTAATCATAAAG
AAGCTTCTTGACCTCTGCAAATTCTGGCCCTGACATGTATTCTAAATACCTTTACAGTTCTAAGTGAGAAATTGTTACATAGGCTTAGAA
GAAGAAAAGACTCCAGTGGGACTGGGATGAGCTGCGTGGTAGCCCTCATAAAGCAGAGAGGTTGGTGGGAGACCTAACTTGGCCTATACT
AGCCTTCCCATTGTTTTCACAATGGGCCTTTTATCCCCTCTGAACTGCTTGCCTGCACTTTTATCTTAGACTTTTTTTTTTGATGAGCAT
AGGTATTTGCTGTGTAAGCCTTATCTAGTGATGCAGAATATCAAGGAGGCCACTTACAATTCTAAGCTGCATCCCTGTATCAAAAATCTC
AGAAGCACCTTGCATCTGATAGAGGCTTGTAAGAGCAGATTGACAGGTGAATGTCACAGGTCCATAAGAAGGACAAATAGATCTAAGTTG
GGCAAAAGATTCTATTTGTTGGTTCTCCCTTCTAAGATGATAATATGCCCCTGCACTTGAGACCTTACTTGAAACTTAAAGAAATGTGAT
TGGTTATGGTGCTGGGAGAAAGGGGTGCACCTGGGAGGACACTGAGAAAGATAAACAAAGGGGAAGGAAGAGAGGACAACCTGTAACTTT
TAGAAAAGGCTACTACTTTTAACTGTTGGAGAAAATGCTATTGCTTGCACTCACAATGTCAATATACATTATCAGAACTTAAGTCCTGGA
GATCAGAGACTGTTTCACCTGAAATTGCTAGCACCTAGAATGGCCCCCGGTTGCGGTAGGCAATCAATAATACTCTTTGAATAATAATGA
GGTTTTCAAAGATTCTGCAAGTCAGACTGGCCTCTGGCTTTGTGATATTATTCTAGAGTCCCAGTAACCCACAATTGTTGATGAGGAGTA
GCAGTAGAATATCAAGGACAGGTGATGAGACAACAAGTCTATCCCGCAAAGGGCAGGGAATAGAGGGTACACTGGAAAGAATTTGAAAAC
AATAATAAAGCTGACTACAAGTTGGTCTATTTTTTATTACCACCATGAGCCAGCAATTCTAAATAATATCAGTGATAAAGTATTCATCCC
CCAAAATATTTTATTGAACTAGGTTCTAAACAATTTCTGAGGTTACTGTTGAGTTTTAAATAATACATATATGTAAGCTTCAAAGTAGCA
CATTTGTACTGTTTTTCTTCAATAATCATTTTACTCCACATGGAAACTAATTCAGGGAATATCCTGCTATATAGTTGGCTCTCAGTACAT
ATGGTGTCAGCTCATGTGTCTCCAGCCCCTGTAGTACTATATATTATTGCATTTAAATGATGGATTTGAAATAAACAATGATTATACCAT
GATTGTAAAGACAAGAAACAGAACCTGAAATACTTCAATTTTTGATTCTATGTGGCAGTTTAGAGTTTTTTAAAACAATTTTTATGTATA

>In-frame_ENST00000295963_ENST00000313851_TCGA-86-A4P7_UBE2K_chr4_39700010_+_FAM216B_chr13_43360898_length(amino acids)=127AA_start in transcript=219_stop in transcript=602
MANIAVQRIKREFKEVLKSEEALNQGQQRYFYSIMRIYNSRPQWEALQTRYIHSLQHQQLLGYITQREALSYALVLRDSTKRASAKVAPQ

--------------------------------------------------------------
>In-frame_ENST00000503368_ENST00000537894_TCGA-86-A4P7_UBE2K_chr4_39700010_+_FAM216B_chr13_43360898_length(transcript)=3306nt_BP=228nt
GATTCCACACTGAGGCGAGCGCGGCGGCCGGGGTGGTAGTGGCAGTGTTCGTGTGCTCAGGTCTGAATCGCCGAGGGAGGAGGCGGTGGA
GGAAGAGGTGGCGGCGGTGGCGGTGGTCGTAGCGGTGGCGGAGGAGGCGGGTACGAATCAGCTGCGGGCGGAGACATGGCCAACATCGCG
GTGCAGCGAATCAAGCGGGAGTTCAAGGAGGTGCTGAAGAGCGAGGAGGCCCTCAACCAAGGGCAACAGCGCTACTTTTACAGCATTATG
AGGATTTACAACTCCAGGCCCCAGTGGGAGGCCCTGCAGACCCGCTACATTCACAGCCTTCAGCACCAACAGCTGCTTGGCTATATTACT
CAACGGGAAGCCTTGTCTTATGCTCTTGTACTTAGAGATTCAACCAAGAGAGCCTCAGCCAAGGTAGCTCCTCAAAGAACCATTCCCCGG
AAAACTTCAGCCATGACAAGAAGATGTCCATCAGTACTACCTGTATCTGTGGTTCTACCTAGGGCCCAAAGTAAAAGGCGCCAAGTGCTC
AGGAACTGAGACTTGGCAGCATTTTCAGAAACAGGAAGTTTGCCCATGTATCCCTGGTATCTAGTGGGTGCTTTGTGCATATTTGTTGAA
TGACAGTGAATAATTTCTAATATAAACCCCAGACCTAAAAATAATCTCTGATTCATATAAATTTTGCCAGCAAGACCAAGAGGTTTAAGA
GCAATGGAGCCGAGAGTAAGCAAGCCTTTCCACATAACATCCGTAGAGTAAAAGGACTTTTAAAGCTTTCAAAGTAAAAGTTTACTTTGG
ATTAGAACCTCCTAGAGAAGTCCCCAAAACAGTGAAAGGGGACAAGTGACCAGAAAGGGCCAGGTCCTCTGTCACTGGGTGACCATTTCT
AGCAAACTATCATGTATACATTTTGAAAATATACAAAACAACACAGGATTAACACTGATCATAGAGGGCTTACAAAATCATATCATCCCA
GCAACAATTGGCCTGTGCATTTCCTTCCTCTCCTCTTCCCCATCAGGCAATGGCATTTAATTTAGCCCCAAAGCTCTTCTAGTTCTCACC
TGGCTTGATCACTTTGTGTCATCTTGAAACATGCACTTATCAGTCTACCACAGCTGAATTGATTTTATGGAGCTGTAACTGGCCTTGACA
TGGGTAAGAGAATTCATGCACACATCTTTCCCTGGCAGCTGAAGAAACACTCTTAAAAGAATATGAAATAACCCCGAAGACTTTATTTCA
TGTCCAACCTTTCTGTGGCCTCAAATGTTTTAAGCAAAGCACATAAAACTATACTAAGCAGTGAGTCAGTTCATCATACAAAGGCATAGT
ATTGCATTAGTTATATTTAATGGTTATTTTCATCACCAGAGATTTTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTTAAAAATCAGTC
ACCAGTGCTCATGTAAAGTCAAAACAAAAAAATGCTTCTTCCTCATCATTGTAAAATACGTCACCCCATTCAAATCAGCTGATGCCTATG
AAAGGAATTGGTTGAGGTGGAATATAAATTTTATTGACAAGGGATGTGCTAACATTGATCTGAAAGGAATATGCTGCATTATTATCAGAA
AAAAAATCATTATCTTCTCTTTGCAATCATGAACATATAAGATATAAAAATTGTCATGAGATTCTGAACACTAGTATTGAGGTCCAGCAC
TACTTCCAAGCTCCTATCTGACTTTTCTTTGGGAGGAATGGGAAGTGTGGTTGGCCCACTAGTCTGATAAGAAGTCTTGGTGAAGCTAAT
ACTGACCTTGCTGGGGTCATTCACTTAATCATAAAGAAGCTTCTTGACCTCTGCAAATTCTGGCCCTGACATGTATTCTAAATACCTTTA
CAGTTCTAAGTGAGAAATTGTTACATAGGCTTAGAAGAAGAAAAGACTCCAGTGGGACTGGGATGAGCTGCGTGGTAGCCCTCATAAAGC
AGAGAGGTTGGTGGGAGACCTAACTTGGCCTATACTAGCCTTCCCATTGTTTTCACAATGGGCCTTTTATCCCCTCTGAACTGCTTGCCT
GCACTTTTATCTTAGACTTTTTTTTTTGATGAGCATAGGTATTTGCTGTGTAAGCCTTATCTAGTGATGCAGAATATCAAGGAGGCCACT
TACAATTCTAAGCTGCATCCCTGTATCAAAAATCTCAGAAGCACCTTGCATCTGATAGAGGCTTGTAAGAGCAGATTGACAGGTGAATGT
CACAGGTCCATAAGAAGGACAAATAGATCTAAGTTGGGCAAAAGATTCTATTTGTTGGTTCTCCCTTCTAAGATGATAATATGCCCCTGC
ACTTGAGACCTTACTTGAAACTTAAAGAAATGTGATTGGTTATGGTGCTGGGAGAAAGGGGTGCACCTGGGAGGACACTGAGAAAGATAA
ACAAAGGGGAAGGAAGAGAGGACAACCTGTAACTTTTAGAAAAGGCTACTACTTTTAACTGTTGGAGAAAATGCTATTGCTTGCACTCAC
AATGTCAATATACATTATCAGAACTTAAGTCCTGGAGATCAGAGACTGTTTCACCTGAAATTGCTAGCACCTAGAATGGCCCCCGGTTGC
GGTAGGCAATCAATAATACTCTTTGAATAATAATGAGGTTTTCAAAGATTCTGCAAGTCAGACTGGCCTCTGGCTTTGTGATATTATTCT
AGAGTCCCAGTAACCCACAATTGTTGATGAGGAGTAGCAGTAGAATATCAAGGACAGGTGATGAGACAACAAGTCTATCCCGCAAAGGGC
AGGGAATAGAGGGTACACTGGAAAGAATTTGAAAACAATAATAAAGCTGACTACAAGTTGGTCTATTTTTTATTACCACCATGAGCCAGC
AATTCTAAATAATATCAGTGATAAAGTATTCATCCCCCAAAATATTTTATTGAACTAGGTTCTAAACAATTTCTGAGGTTACTGTTGAGT
TTTAAATAATACATATATGTAAGCTTCAAAGTAGCACATTTGTACTGTTTTTCTTCAATAATCATTTTACTCCACATGGAAACTAATTCA
GGGAATATCCTGCTATATAGTTGGCTCTCAGTACATATGGTGTCAGCTCATGTGTCTCCAGCCCCTGTAGTACTATATATTATTGCATTT
AAATGATGGATTTGAAATAAACAATGATTATACCATGATTGTAAAGACAAGAAACAGAACCTGAAATACTTCAATTTTTGATTCTATGTG

>In-frame_ENST00000503368_ENST00000537894_TCGA-86-A4P7_UBE2K_chr4_39700010_+_FAM216B_chr13_43360898_length(amino acids)=127AA_start in transcript=165_stop in transcript=548
MANIAVQRIKREFKEVLKSEEALNQGQQRYFYSIMRIYNSRPQWEALQTRYIHSLQHQQLLGYITQREALSYALVLRDSTKRASAKVAPQ

--------------------------------------------------------------
>In-frame_ENST00000503368_ENST00000313851_TCGA-86-A4P7_UBE2K_chr4_39700010_+_FAM216B_chr13_43360898_length(transcript)=3306nt_BP=228nt
GATTCCACACTGAGGCGAGCGCGGCGGCCGGGGTGGTAGTGGCAGTGTTCGTGTGCTCAGGTCTGAATCGCCGAGGGAGGAGGCGGTGGA
GGAAGAGGTGGCGGCGGTGGCGGTGGTCGTAGCGGTGGCGGAGGAGGCGGGTACGAATCAGCTGCGGGCGGAGACATGGCCAACATCGCG
GTGCAGCGAATCAAGCGGGAGTTCAAGGAGGTGCTGAAGAGCGAGGAGGCCCTCAACCAAGGGCAACAGCGCTACTTTTACAGCATTATG
AGGATTTACAACTCCAGGCCCCAGTGGGAGGCCCTGCAGACCCGCTACATTCACAGCCTTCAGCACCAACAGCTGCTTGGCTATATTACT
CAACGGGAAGCCTTGTCTTATGCTCTTGTACTTAGAGATTCAACCAAGAGAGCCTCAGCCAAGGTAGCTCCTCAAAGAACCATTCCCCGG
AAAACTTCAGCCATGACAAGAAGATGTCCATCAGTACTACCTGTATCTGTGGTTCTACCTAGGGCCCAAAGTAAAAGGCGCCAAGTGCTC
AGGAACTGAGACTTGGCAGCATTTTCAGAAACAGGAAGTTTGCCCATGTATCCCTGGTATCTAGTGGGTGCTTTGTGCATATTTGTTGAA
TGACAGTGAATAATTTCTAATATAAACCCCAGACCTAAAAATAATCTCTGATTCATATAAATTTTGCCAGCAAGACCAAGAGGTTTAAGA
GCAATGGAGCCGAGAGTAAGCAAGCCTTTCCACATAACATCCGTAGAGTAAAAGGACTTTTAAAGCTTTCAAAGTAAAAGTTTACTTTGG
ATTAGAACCTCCTAGAGAAGTCCCCAAAACAGTGAAAGGGGACAAGTGACCAGAAAGGGCCAGGTCCTCTGTCACTGGGTGACCATTTCT
AGCAAACTATCATGTATACATTTTGAAAATATACAAAACAACACAGGATTAACACTGATCATAGAGGGCTTACAAAATCATATCATCCCA
GCAACAATTGGCCTGTGCATTTCCTTCCTCTCCTCTTCCCCATCAGGCAATGGCATTTAATTTAGCCCCAAAGCTCTTCTAGTTCTCACC
TGGCTTGATCACTTTGTGTCATCTTGAAACATGCACTTATCAGTCTACCACAGCTGAATTGATTTTATGGAGCTGTAACTGGCCTTGACA
TGGGTAAGAGAATTCATGCACACATCTTTCCCTGGCAGCTGAAGAAACACTCTTAAAAGAATATGAAATAACCCCGAAGACTTTATTTCA
TGTCCAACCTTTCTGTGGCCTCAAATGTTTTAAGCAAAGCACATAAAACTATACTAAGCAGTGAGTCAGTTCATCATACAAAGGCATAGT
ATTGCATTAGTTATATTTAATGGTTATTTTCATCACCAGAGATTTTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTTAAAAATCAGTC
ACCAGTGCTCATGTAAAGTCAAAACAAAAAAATGCTTCTTCCTCATCATTGTAAAATACGTCACCCCATTCAAATCAGCTGATGCCTATG
AAAGGAATTGGTTGAGGTGGAATATAAATTTTATTGACAAGGGATGTGCTAACATTGATCTGAAAGGAATATGCTGCATTATTATCAGAA
AAAAAATCATTATCTTCTCTTTGCAATCATGAACATATAAGATATAAAAATTGTCATGAGATTCTGAACACTAGTATTGAGGTCCAGCAC
TACTTCCAAGCTCCTATCTGACTTTTCTTTGGGAGGAATGGGAAGTGTGGTTGGCCCACTAGTCTGATAAGAAGTCTTGGTGAAGCTAAT
ACTGACCTTGCTGGGGTCATTCACTTAATCATAAAGAAGCTTCTTGACCTCTGCAAATTCTGGCCCTGACATGTATTCTAAATACCTTTA
CAGTTCTAAGTGAGAAATTGTTACATAGGCTTAGAAGAAGAAAAGACTCCAGTGGGACTGGGATGAGCTGCGTGGTAGCCCTCATAAAGC
AGAGAGGTTGGTGGGAGACCTAACTTGGCCTATACTAGCCTTCCCATTGTTTTCACAATGGGCCTTTTATCCCCTCTGAACTGCTTGCCT
GCACTTTTATCTTAGACTTTTTTTTTTGATGAGCATAGGTATTTGCTGTGTAAGCCTTATCTAGTGATGCAGAATATCAAGGAGGCCACT
TACAATTCTAAGCTGCATCCCTGTATCAAAAATCTCAGAAGCACCTTGCATCTGATAGAGGCTTGTAAGAGCAGATTGACAGGTGAATGT
CACAGGTCCATAAGAAGGACAAATAGATCTAAGTTGGGCAAAAGATTCTATTTGTTGGTTCTCCCTTCTAAGATGATAATATGCCCCTGC
ACTTGAGACCTTACTTGAAACTTAAAGAAATGTGATTGGTTATGGTGCTGGGAGAAAGGGGTGCACCTGGGAGGACACTGAGAAAGATAA
ACAAAGGGGAAGGAAGAGAGGACAACCTGTAACTTTTAGAAAAGGCTACTACTTTTAACTGTTGGAGAAAATGCTATTGCTTGCACTCAC
AATGTCAATATACATTATCAGAACTTAAGTCCTGGAGATCAGAGACTGTTTCACCTGAAATTGCTAGCACCTAGAATGGCCCCCGGTTGC
GGTAGGCAATCAATAATACTCTTTGAATAATAATGAGGTTTTCAAAGATTCTGCAAGTCAGACTGGCCTCTGGCTTTGTGATATTATTCT
AGAGTCCCAGTAACCCACAATTGTTGATGAGGAGTAGCAGTAGAATATCAAGGACAGGTGATGAGACAACAAGTCTATCCCGCAAAGGGC
AGGGAATAGAGGGTACACTGGAAAGAATTTGAAAACAATAATAAAGCTGACTACAAGTTGGTCTATTTTTTATTACCACCATGAGCCAGC
AATTCTAAATAATATCAGTGATAAAGTATTCATCCCCCAAAATATTTTATTGAACTAGGTTCTAAACAATTTCTGAGGTTACTGTTGAGT
TTTAAATAATACATATATGTAAGCTTCAAAGTAGCACATTTGTACTGTTTTTCTTCAATAATCATTTTACTCCACATGGAAACTAATTCA
GGGAATATCCTGCTATATAGTTGGCTCTCAGTACATATGGTGTCAGCTCATGTGTCTCCAGCCCCTGTAGTACTATATATTATTGCATTT
AAATGATGGATTTGAAATAAACAATGATTATACCATGATTGTAAAGACAAGAAACAGAACCTGAAATACTTCAATTTTTGATTCTATGTG

>In-frame_ENST00000503368_ENST00000313851_TCGA-86-A4P7_UBE2K_chr4_39700010_+_FAM216B_chr13_43360898_length(amino acids)=127AA_start in transcript=165_stop in transcript=548
MANIAVQRIKREFKEVLKSEEALNQGQQRYFYSIMRIYNSRPQWEALQTRYIHSLQHQQLLGYITQREALSYALVLRDSTKRASAKVAPQ

--------------------------------------------------------------
>In-frame_ENST00000445950_ENST00000537894_TCGA-86-A4P7_UBE2K_chr4_39700010_+_FAM216B_chr13_43360898_length(transcript)=3293nt_BP=215nt
GGCGAGCGCGGCGGCCGGGGTGGTAGTGGCAGTGTTCGTGTGCTCAGGTCTGAATCGCCGAGGGAGGAGGCGGTGGAGGAAGAGGTGGCG
GCGGTGGCGGTGGTCGTAGCGGTGGCGGAGGAGGCGGGTACGAATCAGCTGCGGGCGGAGACATGGCCAACATCGCGGTGCAGCGAATCA
AGCGGGAGTTCAAGGAGGTGCTGAAGAGCGAGGAGGCCCTCAACCAAGGGCAACAGCGCTACTTTTACAGCATTATGAGGATTTACAACT
CCAGGCCCCAGTGGGAGGCCCTGCAGACCCGCTACATTCACAGCCTTCAGCACCAACAGCTGCTTGGCTATATTACTCAACGGGAAGCCT
TGTCTTATGCTCTTGTACTTAGAGATTCAACCAAGAGAGCCTCAGCCAAGGTAGCTCCTCAAAGAACCATTCCCCGGAAAACTTCAGCCA
TGACAAGAAGATGTCCATCAGTACTACCTGTATCTGTGGTTCTACCTAGGGCCCAAAGTAAAAGGCGCCAAGTGCTCAGGAACTGAGACT
TGGCAGCATTTTCAGAAACAGGAAGTTTGCCCATGTATCCCTGGTATCTAGTGGGTGCTTTGTGCATATTTGTTGAATGACAGTGAATAA
TTTCTAATATAAACCCCAGACCTAAAAATAATCTCTGATTCATATAAATTTTGCCAGCAAGACCAAGAGGTTTAAGAGCAATGGAGCCGA
GAGTAAGCAAGCCTTTCCACATAACATCCGTAGAGTAAAAGGACTTTTAAAGCTTTCAAAGTAAAAGTTTACTTTGGATTAGAACCTCCT
AGAGAAGTCCCCAAAACAGTGAAAGGGGACAAGTGACCAGAAAGGGCCAGGTCCTCTGTCACTGGGTGACCATTTCTAGCAAACTATCAT
GTATACATTTTGAAAATATACAAAACAACACAGGATTAACACTGATCATAGAGGGCTTACAAAATCATATCATCCCAGCAACAATTGGCC
TGTGCATTTCCTTCCTCTCCTCTTCCCCATCAGGCAATGGCATTTAATTTAGCCCCAAAGCTCTTCTAGTTCTCACCTGGCTTGATCACT
TTGTGTCATCTTGAAACATGCACTTATCAGTCTACCACAGCTGAATTGATTTTATGGAGCTGTAACTGGCCTTGACATGGGTAAGAGAAT
TCATGCACACATCTTTCCCTGGCAGCTGAAGAAACACTCTTAAAAGAATATGAAATAACCCCGAAGACTTTATTTCATGTCCAACCTTTC
TGTGGCCTCAAATGTTTTAAGCAAAGCACATAAAACTATACTAAGCAGTGAGTCAGTTCATCATACAAAGGCATAGTATTGCATTAGTTA
TATTTAATGGTTATTTTCATCACCAGAGATTTTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTTAAAAATCAGTCACCAGTGCTCATG
TAAAGTCAAAACAAAAAAATGCTTCTTCCTCATCATTGTAAAATACGTCACCCCATTCAAATCAGCTGATGCCTATGAAAGGAATTGGTT
GAGGTGGAATATAAATTTTATTGACAAGGGATGTGCTAACATTGATCTGAAAGGAATATGCTGCATTATTATCAGAAAAAAAATCATTAT
CTTCTCTTTGCAATCATGAACATATAAGATATAAAAATTGTCATGAGATTCTGAACACTAGTATTGAGGTCCAGCACTACTTCCAAGCTC
CTATCTGACTTTTCTTTGGGAGGAATGGGAAGTGTGGTTGGCCCACTAGTCTGATAAGAAGTCTTGGTGAAGCTAATACTGACCTTGCTG
GGGTCATTCACTTAATCATAAAGAAGCTTCTTGACCTCTGCAAATTCTGGCCCTGACATGTATTCTAAATACCTTTACAGTTCTAAGTGA
GAAATTGTTACATAGGCTTAGAAGAAGAAAAGACTCCAGTGGGACTGGGATGAGCTGCGTGGTAGCCCTCATAAAGCAGAGAGGTTGGTG
GGAGACCTAACTTGGCCTATACTAGCCTTCCCATTGTTTTCACAATGGGCCTTTTATCCCCTCTGAACTGCTTGCCTGCACTTTTATCTT
AGACTTTTTTTTTTGATGAGCATAGGTATTTGCTGTGTAAGCCTTATCTAGTGATGCAGAATATCAAGGAGGCCACTTACAATTCTAAGC
TGCATCCCTGTATCAAAAATCTCAGAAGCACCTTGCATCTGATAGAGGCTTGTAAGAGCAGATTGACAGGTGAATGTCACAGGTCCATAA
GAAGGACAAATAGATCTAAGTTGGGCAAAAGATTCTATTTGTTGGTTCTCCCTTCTAAGATGATAATATGCCCCTGCACTTGAGACCTTA
CTTGAAACTTAAAGAAATGTGATTGGTTATGGTGCTGGGAGAAAGGGGTGCACCTGGGAGGACACTGAGAAAGATAAACAAAGGGGAAGG
AAGAGAGGACAACCTGTAACTTTTAGAAAAGGCTACTACTTTTAACTGTTGGAGAAAATGCTATTGCTTGCACTCACAATGTCAATATAC
ATTATCAGAACTTAAGTCCTGGAGATCAGAGACTGTTTCACCTGAAATTGCTAGCACCTAGAATGGCCCCCGGTTGCGGTAGGCAATCAA
TAATACTCTTTGAATAATAATGAGGTTTTCAAAGATTCTGCAAGTCAGACTGGCCTCTGGCTTTGTGATATTATTCTAGAGTCCCAGTAA
CCCACAATTGTTGATGAGGAGTAGCAGTAGAATATCAAGGACAGGTGATGAGACAACAAGTCTATCCCGCAAAGGGCAGGGAATAGAGGG
TACACTGGAAAGAATTTGAAAACAATAATAAAGCTGACTACAAGTTGGTCTATTTTTTATTACCACCATGAGCCAGCAATTCTAAATAAT
ATCAGTGATAAAGTATTCATCCCCCAAAATATTTTATTGAACTAGGTTCTAAACAATTTCTGAGGTTACTGTTGAGTTTTAAATAATACA
TATATGTAAGCTTCAAAGTAGCACATTTGTACTGTTTTTCTTCAATAATCATTTTACTCCACATGGAAACTAATTCAGGGAATATCCTGC
TATATAGTTGGCTCTCAGTACATATGGTGTCAGCTCATGTGTCTCCAGCCCCTGTAGTACTATATATTATTGCATTTAAATGATGGATTT
GAAATAAACAATGATTATACCATGATTGTAAAGACAAGAAACAGAACCTGAAATACTTCAATTTTTGATTCTATGTGGCAGTTTAGAGTT

>In-frame_ENST00000445950_ENST00000537894_TCGA-86-A4P7_UBE2K_chr4_39700010_+_FAM216B_chr13_43360898_length(amino acids)=127AA_start in transcript=152_stop in transcript=535
MANIAVQRIKREFKEVLKSEEALNQGQQRYFYSIMRIYNSRPQWEALQTRYIHSLQHQQLLGYITQREALSYALVLRDSTKRASAKVAPQ

--------------------------------------------------------------
>In-frame_ENST00000445950_ENST00000313851_TCGA-86-A4P7_UBE2K_chr4_39700010_+_FAM216B_chr13_43360898_length(transcript)=3293nt_BP=215nt
GGCGAGCGCGGCGGCCGGGGTGGTAGTGGCAGTGTTCGTGTGCTCAGGTCTGAATCGCCGAGGGAGGAGGCGGTGGAGGAAGAGGTGGCG
GCGGTGGCGGTGGTCGTAGCGGTGGCGGAGGAGGCGGGTACGAATCAGCTGCGGGCGGAGACATGGCCAACATCGCGGTGCAGCGAATCA
AGCGGGAGTTCAAGGAGGTGCTGAAGAGCGAGGAGGCCCTCAACCAAGGGCAACAGCGCTACTTTTACAGCATTATGAGGATTTACAACT
CCAGGCCCCAGTGGGAGGCCCTGCAGACCCGCTACATTCACAGCCTTCAGCACCAACAGCTGCTTGGCTATATTACTCAACGGGAAGCCT
TGTCTTATGCTCTTGTACTTAGAGATTCAACCAAGAGAGCCTCAGCCAAGGTAGCTCCTCAAAGAACCATTCCCCGGAAAACTTCAGCCA
TGACAAGAAGATGTCCATCAGTACTACCTGTATCTGTGGTTCTACCTAGGGCCCAAAGTAAAAGGCGCCAAGTGCTCAGGAACTGAGACT
TGGCAGCATTTTCAGAAACAGGAAGTTTGCCCATGTATCCCTGGTATCTAGTGGGTGCTTTGTGCATATTTGTTGAATGACAGTGAATAA
TTTCTAATATAAACCCCAGACCTAAAAATAATCTCTGATTCATATAAATTTTGCCAGCAAGACCAAGAGGTTTAAGAGCAATGGAGCCGA
GAGTAAGCAAGCCTTTCCACATAACATCCGTAGAGTAAAAGGACTTTTAAAGCTTTCAAAGTAAAAGTTTACTTTGGATTAGAACCTCCT
AGAGAAGTCCCCAAAACAGTGAAAGGGGACAAGTGACCAGAAAGGGCCAGGTCCTCTGTCACTGGGTGACCATTTCTAGCAAACTATCAT
GTATACATTTTGAAAATATACAAAACAACACAGGATTAACACTGATCATAGAGGGCTTACAAAATCATATCATCCCAGCAACAATTGGCC
TGTGCATTTCCTTCCTCTCCTCTTCCCCATCAGGCAATGGCATTTAATTTAGCCCCAAAGCTCTTCTAGTTCTCACCTGGCTTGATCACT
TTGTGTCATCTTGAAACATGCACTTATCAGTCTACCACAGCTGAATTGATTTTATGGAGCTGTAACTGGCCTTGACATGGGTAAGAGAAT
TCATGCACACATCTTTCCCTGGCAGCTGAAGAAACACTCTTAAAAGAATATGAAATAACCCCGAAGACTTTATTTCATGTCCAACCTTTC
TGTGGCCTCAAATGTTTTAAGCAAAGCACATAAAACTATACTAAGCAGTGAGTCAGTTCATCATACAAAGGCATAGTATTGCATTAGTTA
TATTTAATGGTTATTTTCATCACCAGAGATTTTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTTAAAAATCAGTCACCAGTGCTCATG
TAAAGTCAAAACAAAAAAATGCTTCTTCCTCATCATTGTAAAATACGTCACCCCATTCAAATCAGCTGATGCCTATGAAAGGAATTGGTT
GAGGTGGAATATAAATTTTATTGACAAGGGATGTGCTAACATTGATCTGAAAGGAATATGCTGCATTATTATCAGAAAAAAAATCATTAT
CTTCTCTTTGCAATCATGAACATATAAGATATAAAAATTGTCATGAGATTCTGAACACTAGTATTGAGGTCCAGCACTACTTCCAAGCTC
CTATCTGACTTTTCTTTGGGAGGAATGGGAAGTGTGGTTGGCCCACTAGTCTGATAAGAAGTCTTGGTGAAGCTAATACTGACCTTGCTG
GGGTCATTCACTTAATCATAAAGAAGCTTCTTGACCTCTGCAAATTCTGGCCCTGACATGTATTCTAAATACCTTTACAGTTCTAAGTGA
GAAATTGTTACATAGGCTTAGAAGAAGAAAAGACTCCAGTGGGACTGGGATGAGCTGCGTGGTAGCCCTCATAAAGCAGAGAGGTTGGTG
GGAGACCTAACTTGGCCTATACTAGCCTTCCCATTGTTTTCACAATGGGCCTTTTATCCCCTCTGAACTGCTTGCCTGCACTTTTATCTT
AGACTTTTTTTTTTGATGAGCATAGGTATTTGCTGTGTAAGCCTTATCTAGTGATGCAGAATATCAAGGAGGCCACTTACAATTCTAAGC
TGCATCCCTGTATCAAAAATCTCAGAAGCACCTTGCATCTGATAGAGGCTTGTAAGAGCAGATTGACAGGTGAATGTCACAGGTCCATAA
GAAGGACAAATAGATCTAAGTTGGGCAAAAGATTCTATTTGTTGGTTCTCCCTTCTAAGATGATAATATGCCCCTGCACTTGAGACCTTA
CTTGAAACTTAAAGAAATGTGATTGGTTATGGTGCTGGGAGAAAGGGGTGCACCTGGGAGGACACTGAGAAAGATAAACAAAGGGGAAGG
AAGAGAGGACAACCTGTAACTTTTAGAAAAGGCTACTACTTTTAACTGTTGGAGAAAATGCTATTGCTTGCACTCACAATGTCAATATAC
ATTATCAGAACTTAAGTCCTGGAGATCAGAGACTGTTTCACCTGAAATTGCTAGCACCTAGAATGGCCCCCGGTTGCGGTAGGCAATCAA
TAATACTCTTTGAATAATAATGAGGTTTTCAAAGATTCTGCAAGTCAGACTGGCCTCTGGCTTTGTGATATTATTCTAGAGTCCCAGTAA
CCCACAATTGTTGATGAGGAGTAGCAGTAGAATATCAAGGACAGGTGATGAGACAACAAGTCTATCCCGCAAAGGGCAGGGAATAGAGGG
TACACTGGAAAGAATTTGAAAACAATAATAAAGCTGACTACAAGTTGGTCTATTTTTTATTACCACCATGAGCCAGCAATTCTAAATAAT
ATCAGTGATAAAGTATTCATCCCCCAAAATATTTTATTGAACTAGGTTCTAAACAATTTCTGAGGTTACTGTTGAGTTTTAAATAATACA
TATATGTAAGCTTCAAAGTAGCACATTTGTACTGTTTTTCTTCAATAATCATTTTACTCCACATGGAAACTAATTCAGGGAATATCCTGC
TATATAGTTGGCTCTCAGTACATATGGTGTCAGCTCATGTGTCTCCAGCCCCTGTAGTACTATATATTATTGCATTTAAATGATGGATTT
GAAATAAACAATGATTATACCATGATTGTAAAGACAAGAAACAGAACCTGAAATACTTCAATTTTTGATTCTATGTGGCAGTTTAGAGTT

>In-frame_ENST00000445950_ENST00000313851_TCGA-86-A4P7_UBE2K_chr4_39700010_+_FAM216B_chr13_43360898_length(amino acids)=127AA_start in transcript=152_stop in transcript=535
MANIAVQRIKREFKEVLKSEEALNQGQQRYFYSIMRIYNSRPQWEALQTRYIHSLQHQQLLGYITQREALSYALVLRDSTKRASAKVAPQ

--------------------------------------------------------------

Top

Fusion Gene PPI Analysis for UBE2K-FAM216B


check button Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in

ChiPPI page.


check button Protein-protein interactors with each fusion partner protein in wild-type (BIOGRID-3.4.160)
HgeneHgene's interactorsTgeneTgene's interactors


check button - Retained PPIs in in-frame fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenStill interaction with


check button - Lost PPIs in in-frame fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenInteraction lost with


check button - Retained PPIs, but lost function due to frame-shift fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenInteraction lost with


Top

Related Drugs for UBE2K-FAM216B


check button Drugs targeting genes involved in this fusion gene.
(DrugBank Version 5.1.8 2021-05-08)
PartnerGeneUniProtAccDrugBank IDDrug nameDrug activityDrug typeDrug status

Top

Related Diseases for UBE2K-FAM216B


check button Diseases associated with fusion partners.
(DisGeNet 4.0)
PartnerGeneDisease IDDisease name# pubmedsSource