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Center for Computational Systems Medicine
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Fusion Gene Summary

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Fusion Gene ORF analysis

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Fusion Genomic Features

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Fusion Protein Features

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Fusion Gene Sequence

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Fusion Gene PPI analysis

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Related Drugs

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Related Diseases

Fusion gene:USP25-KIF3A (FusionGDB2 ID:96635)

Fusion Gene Summary for USP25-KIF3A

check button Fusion gene summary
Fusion gene informationFusion gene name: USP25-KIF3A
Fusion gene ID: 96635
HgeneTgene
Gene symbol

USP25

KIF3A

Gene ID

29761

11127

Gene nameubiquitin specific peptidase 25kinesin family member 3A
SynonymsUSP21FLA10|KLP-20
Cytomap

21q21.1

5q31.1

Type of geneprotein-codingprotein-coding
Descriptionubiquitin carboxyl-terminal hydrolase 25USP on chromosome 21deubiquitinating enzyme 25ubiquitin thioesterase 25ubiquitin thiolesterase 25ubiquitin-specific processing protease 25kinesin-like protein KIF3Akinesin family protein 3Amicrotubule plus end-directed kinesin motor 3A
Modification date2020031320200313
UniProtAcc.

Q9Y496

Ensembl transtripts involved in fusion geneENST00000351097, ENST00000285679, 
ENST00000285681, ENST00000400183, 
ENST00000547201, 		ENST00000378746, 
ENST00000378735, ENST00000403231, 
ENST00000487055, 
Fusion gene scores* DoF score13 X 10 X 9=11709 X 5 X 6=270
# samples 139
** MAII scorelog2(13/1170*10)=-3.16992500144231
possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs).
DoF>8 and MAII<0		log2(9/270*10)=-1.58496250072116
possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs).
DoF>8 and MAII<0
Context

PubMed: USP25 [Title/Abstract] AND KIF3A [Title/Abstract] AND fusion [Title/Abstract]

Most frequent breakpointUSP25(17138460)-KIF3A(132039311), # samples:1
Anticipated loss of major functional domain due to fusion event.
* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

check button Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez
PartnerGeneGO IDGO termPubMed ID
HgeneUSP25

GO:0016579

protein deubiquitination

22590560


check buttonFusion gene breakpoints across USP25 (5'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

check buttonFusion gene breakpoints across KIF3A (3'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

check button Fusion gene information from two resources (ChiTars 5.0 and ChimerDB 4.0)
* All genome coordinats were lifted-over on hg19.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.
SourceDiseaseSampleHgeneHchrHbpHstrandTgeneTchrTbpTstrand
ChimerDB4ESCATCGA-L5-A8NEUSP25chr21

17138460

+KIF3Achr5

132039311

-


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Fusion Gene ORF analysis for USP25-KIF3A

check button Open reading frame (ORF) analsis of fusion genes based on Ensembl gene isoform structure.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.
ORFHenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrand
In-frameENST00000351097ENST00000378746USP25chr21

17138460

+KIF3Achr5

132039311

-
In-frameENST00000351097ENST00000378735USP25chr21

17138460

+KIF3Achr5

132039311

-
In-frameENST00000351097ENST00000403231USP25chr21

17138460

+KIF3Achr5

132039311

-
5CDS-intronENST00000351097ENST00000487055USP25chr21

17138460

+KIF3Achr5

132039311

-
In-frameENST00000285679ENST00000378746USP25chr21

17138460

+KIF3Achr5

132039311

-
In-frameENST00000285679ENST00000378735USP25chr21

17138460

+KIF3Achr5

132039311

-
In-frameENST00000285679ENST00000403231USP25chr21

17138460

+KIF3Achr5

132039311

-
5CDS-intronENST00000285679ENST00000487055USP25chr21

17138460

+KIF3Achr5

132039311

-
In-frameENST00000285681ENST00000378746USP25chr21

17138460

+KIF3Achr5

132039311

-
In-frameENST00000285681ENST00000378735USP25chr21

17138460

+KIF3Achr5

132039311

-
In-frameENST00000285681ENST00000403231USP25chr21

17138460

+KIF3Achr5

132039311

-
5CDS-intronENST00000285681ENST00000487055USP25chr21

17138460

+KIF3Achr5

132039311

-
In-frameENST00000400183ENST00000378746USP25chr21

17138460

+KIF3Achr5

132039311

-
In-frameENST00000400183ENST00000378735USP25chr21

17138460

+KIF3Achr5

132039311

-
In-frameENST00000400183ENST00000403231USP25chr21

17138460

+KIF3Achr5

132039311

-
5CDS-intronENST00000400183ENST00000487055USP25chr21

17138460

+KIF3Achr5

132039311

-
intron-3CDSENST00000547201ENST00000378746USP25chr21

17138460

+KIF3Achr5

132039311

-
intron-3CDSENST00000547201ENST00000378735USP25chr21

17138460

+KIF3Achr5

132039311

-
intron-3CDSENST00000547201ENST00000403231USP25chr21

17138460

+KIF3Achr5

132039311

-
intron-intronENST00000547201ENST00000487055USP25chr21

17138460

+KIF3Achr5

132039311

-

check buttonORFfinder result based on the fusion transcript sequence of in-frame fusion genes.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandSeq length
(transcript)		BP loci
(transcript)		Predicted start
(transcript)		Predicted stop
(transcript)		Seq length
(amino acids)
ENST00000351097USP25chr2117138460+ENST00000378746KIF3Achr5132039311-55156371891508439
ENST00000351097USP25chr2117138460+ENST00000378735KIF3Achr5132039311-25136371891508439
ENST00000351097USP25chr2117138460+ENST00000403231KIF3Achr5132039311-15096371891508439
ENST00000285679USP25chr2117138460+ENST00000378746KIF3Achr5132039311-55156371891508439
ENST00000285679USP25chr2117138460+ENST00000378735KIF3Achr5132039311-25136371891508439
ENST00000285679USP25chr2117138460+ENST00000403231KIF3Achr5132039311-15096371891508439
ENST00000285681USP25chr2117138460+ENST00000378746KIF3Achr5132039311-55156371891508439
ENST00000285681USP25chr2117138460+ENST00000378735KIF3Achr5132039311-25136371891508439
ENST00000285681USP25chr2117138460+ENST00000403231KIF3Achr5132039311-15096371891508439
ENST00000400183USP25chr2117138460+ENST00000378746KIF3Achr5132039311-5363485371356439
ENST00000400183USP25chr2117138460+ENST00000378735KIF3Achr5132039311-2361485371356439
ENST00000400183USP25chr2117138460+ENST00000403231KIF3Achr5132039311-1357485371356440

check buttonDeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandNo-coding scoreCoding score
ENST00000351097ENST00000378746USP25chr2117138460+KIF3Achr5132039311-0.0001108270.99988914
ENST00000351097ENST00000378735USP25chr2117138460+KIF3Achr5132039311-0.0003076230.99969244
ENST00000351097ENST00000403231USP25chr2117138460+KIF3Achr5132039311-0.0018458010.9981542
ENST00000285679ENST00000378746USP25chr2117138460+KIF3Achr5132039311-0.0001108270.99988914
ENST00000285679ENST00000378735USP25chr2117138460+KIF3Achr5132039311-0.0003076230.99969244
ENST00000285679ENST00000403231USP25chr2117138460+KIF3Achr5132039311-0.0018458010.9981542
ENST00000285681ENST00000378746USP25chr2117138460+KIF3Achr5132039311-0.0001108270.99988914
ENST00000285681ENST00000378735USP25chr2117138460+KIF3Achr5132039311-0.0003076230.99969244
ENST00000285681ENST00000403231USP25chr2117138460+KIF3Achr5132039311-0.0018458010.9981542
ENST00000400183ENST00000378746USP25chr2117138460+KIF3Achr5132039311-0.0001071050.99989283
ENST00000400183ENST00000378735USP25chr2117138460+KIF3Achr5132039311-0.0002794950.9997205
ENST00000400183ENST00000403231USP25chr2117138460+KIF3Achr5132039311-0.0014952840.99850476

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Fusion Genomic Features for USP25-KIF3A


check buttonFusionAI prediction of the potential fusion gene breakpoint based on the pre-mature RNA sequence context (+/- 5kb of individual partner genes, total 20kb length sequence). FusionAI is a fusion gene breakpoint classifier based on convolutional neural network by comparing the fusion positive and negative sequence context of ~ 20K fusion gene data. From here, we can have the relative potentency of the 20K genomic sequence how individual sequnce will be likely used as the gene fusion breakpoints.
HgeneHchrHbpHstrandTgeneTchrTbpTstrand1-pp (fusion gene breakpoint)

check buttonDistribution of 44 human genomic features loci across 20kb length fusion breakpoint regions. We integrated a total of 44 different types of human genomic feature loci information across five big categories including virus integration sites, repeats, structural variants, chromatin states, and gene expression regulation. More details are in help page.

check buttonDistribution of 44 human genomic features loci across 20kb length fusion breakpoint regions that are ovelapped with the top 1% feature importance score regions. More details are in help page.

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Fusion Protein Features for USP25-KIF3A


check button Go to

FGviewer for the breakpoints of chr21:17138460-chr5:132039311

.
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
FGviewer

check buttonMain function of each fusion partner protein. (from UniProt)
HgeneTgene
.KIF3A

Q9Y496

FUNCTION: Might normally function as a transcriptional repressor. EWS-fusion-proteins (EFPS) may play a role in the tumorigenic process. They may disturb gene expression by mimicking, or interfering with the normal function of CTD-POLII within the transcription initiation complex. They may also contribute to an aberrant activation of the fusion protein target genes.FUNCTION: Microtubule-based anterograde translocator for membranous organelles. Plus end-directed microtubule sliding activity in vitro. Plays a role in primary cilia formation. Plays a role in centriole cohesion and subdistal appendage organization and function. Regulates the formation of the subdistal appendage via recruitment of DCTN1 to the centriole. Also required for ciliary basal feet formation and microtubule anchoring to mother centriole. {ECO:0000250|UniProtKB:P28741}.

check buttonRetention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at

download page


* Minus value of BPloci means that the break pointn is located before the CDS.
- In-frame and retained protein feature among the 13 regional features.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note
HgeneUSP25chr21:17138460chr5:132039311ENST00000285679+32414_5789.333333333333331056.0DomainNote=UBA-like
HgeneUSP25chr21:17138460chr5:132039311ENST00000285681+32514_5789.333333333333331088.0DomainNote=UBA-like
HgeneUSP25chr21:17138460chr5:132039311ENST00000400183+32614_5789.333333333333331126.0DomainNote=UBA-like
TgeneKIF3Achr21:17138460chr5:132039311ENST00000378746817697_699409.3333333333333700.0RegionNote=Globular

- In-frame and not-retained protein feature among the 13 regional features.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note
HgeneUSP25chr21:17138460chr5:132039311ENST00000285679+324541_57889.333333333333331056.0Coiled coilOntology_term=ECO:0000255
HgeneUSP25chr21:17138460chr5:132039311ENST00000285679+324684_71789.333333333333331056.0Coiled coilOntology_term=ECO:0000255
HgeneUSP25chr21:17138460chr5:132039311ENST00000285681+325541_57889.333333333333331088.0Coiled coilOntology_term=ECO:0000255
HgeneUSP25chr21:17138460chr5:132039311ENST00000285681+325684_71789.333333333333331088.0Coiled coilOntology_term=ECO:0000255
HgeneUSP25chr21:17138460chr5:132039311ENST00000400183+326541_57889.333333333333331126.0Coiled coilOntology_term=ECO:0000255
HgeneUSP25chr21:17138460chr5:132039311ENST00000400183+326684_71789.333333333333331126.0Coiled coilOntology_term=ECO:0000255
HgeneUSP25chr21:17138460chr5:132039311ENST00000285679+324123_14089.333333333333331056.0DomainUIM 2
HgeneUSP25chr21:17138460chr5:132039311ENST00000285679+324169_65789.333333333333331056.0DomainNote=USP
HgeneUSP25chr21:17138460chr5:132039311ENST00000285679+32497_11689.333333333333331056.0DomainUIM 1
HgeneUSP25chr21:17138460chr5:132039311ENST00000285681+325123_14089.333333333333331088.0DomainUIM 2
HgeneUSP25chr21:17138460chr5:132039311ENST00000285681+325169_65789.333333333333331088.0DomainNote=USP
HgeneUSP25chr21:17138460chr5:132039311ENST00000285681+32597_11689.333333333333331088.0DomainUIM 1
HgeneUSP25chr21:17138460chr5:132039311ENST00000400183+326123_14089.333333333333331126.0DomainUIM 2
HgeneUSP25chr21:17138460chr5:132039311ENST00000400183+326169_65789.333333333333331126.0DomainNote=USP
HgeneUSP25chr21:17138460chr5:132039311ENST00000400183+32697_11689.333333333333331126.0DomainUIM 1
HgeneUSP25chr21:17138460chr5:132039311ENST00000285679+32489_9589.333333333333331056.0MotifNote=Required for SUMO paralog-specific binding
HgeneUSP25chr21:17138460chr5:132039311ENST00000285681+32589_9589.333333333333331088.0MotifNote=Required for SUMO paralog-specific binding
HgeneUSP25chr21:17138460chr5:132039311ENST00000400183+32689_9589.333333333333331126.0MotifNote=Required for SUMO paralog-specific binding
TgeneKIF3Achr21:17138460chr5:132039311ENST00000378746817355_590409.3333333333333700.0Coiled coilOntology_term=ECO:0000255
TgeneKIF3Achr21:17138460chr5:132039311ENST0000037874681714_345409.3333333333333700.0DomainKinesin motor
TgeneKIF3Achr21:17138460chr5:132039311ENST00000378746817100_107409.3333333333333700.0Nucleotide bindingATP


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Fusion Gene Sequence for USP25-KIF3A


check button For in-frame fusion transcripts, we provide the fusion transcript sequences and fusion amino acid sequences. To have fusion amino acid sequence, we ran ORFfinder and chose the longest ORF among the all predicted ones.
>In-frame_ENST00000351097_ENST00000378746_TCGA-L5-A8NE_USP25_chr21_17138460_+_KIF3A_chr5_132039311_length(transcript)=5515nt_BP=637nt
AAGCAGCCCGCGGACCGGCAGCAAAGGAACGTGCGAACGCGTGACGCCGCCCGACTGGCTCGCGCTCTCCCGTGCCCCGGCGTCCTCCGC
CCGCTCATGGCCCGGGCCGCCGCGGACGAGCGGCGCTGAGGCGGGCCGCGTGGAGACGTGAGGCGGCCGCCGTGGCCCTCACAGTCGGCG
TTTCGCCGCCTGCCCGCGGTGCCCGCGCACGCCGGCCGCCATCGCCTTCGCGCCTGGCTGGCGGGGGCGCTGTCCTCCCAGGCCGTCCGC
GCCGCTCCCTGGAGCTCGGCGGAGCGCGGCAGCCAGGGCCGGCGGAGGCGCGAGGAGCCGGGCGCCACCGCCGCCGCCGCCGCCGCCGCC
GCGGGGGCCATGACCGTGGAGCAGAACGTGCTGCAGCAGAGCGCGGCGCAGAAGCACCAGCAGACGTTTTTGAATCAACTGAGAGAAATT
ACGGGGATTAATGACACCCAGATACTACAGCAAGCCTTGAAGGATAGTAATGGAAACTTGGAATTAGCAGTGGCTTTCCTTACTGCGAAG
AATGCTAAGACCCCTCAGCAGGAGGAGACAACTTACTACCAAACAGCACTTCCTGGCAATGATAGATACATCAGTGTGGGAAGCCAAGCA
GATACAAGAAAAAAGAAAGTCTCCCCAGACAAGATGATTGAAATGCAAGCAAAAATTGATGAGGAGAGAAAAGCACTTGAAACAAAGCTC
GACATGGAAGAAGAAGAAAGAAACAAGGCTAGAGCTGAATTAGAGAAACGGGAAAAAGATCTTCTTAAAGCCCAACAAGAGCATCAGTCT
TTGCTGGAAAAATTATCTGCCCTGGAAAAGAAGGTAATTGTTGGTGGGGTTGACTTGTTGGCCAAAGCTGAGGAACAAGAGAAACTTCTT
GAAGAATCTAACATGGAACTGGAAGAAAGGAGGAAAAGAGCAGAGCAACTTCGCAGAGAACTTGAGGAAAAAGAGCAAGAACGCTTGGAT
ATTGAAGAAAAATATACCAGTTTGCAAGAGGAAGCACAGGGAAAGACCAAGAAGTTAAAGAAAGTTTGGACTATGCTGATGGCTGCAAAG
TCAGAGATGGCTGATCTCCAACAAGAACATCAGAGGGAAATTGAAGGCCTACTGGAGAACATTCGGCAACTTAGCCGGGAGCTTCGACTT
CAGATGCTTATTATTGATAACTTTATACCTCGGGATTATCAGGAAATGATTGAAAACTATGTCCATTGGAATGAAGACATAGGAGAATGG
CAGCTAAAATGTGTTGCTTATACAGGAAATAACATGAGGAAGCAAACCCCAGTACCTGATAAAAAGGAGAAAGATCCCTTTGAGGTGGAC
CTTTCTCACGTGTATCTTGCCTATACTGAGGAGAGTCTGCGTCAGTCTTTGATGAAACTAGAAAGACCACGAACTTCAAAGGGGAAAGCA
AGGCCAAAGACAGGGAGAAGAAAGCGTTCTGCAAAGCCTGAAACTGTAATTGACTCTTTACTGCAGTAAATGTTACAGACTTAAAGTCAC
AATAAAAATTAGTGATATTCTCATGCCTGGACAAAATCTTTAATTAAAACAGTGAAGACTTCTCTATAATTTCAATTAATGGAAGTTGTA
GATCAATGAATAAGACTGGAAATAATAATTTGCTTAAGAACTTTTAGTCTACATATATTAATATAACATTAAAATTGTACAACTGGTGAT
TGTTCAAATTGTTGTACTACTACTCTTCAATTGTACTGTGCAGGGAAGTTGGGGGAACAGTTCATACTATAGAGAGTTACAGTTTAGATG
TATGTGTAAATCGATTAGCTATTTATCCAACTACTGTATATTTAGGTAACTAGAATTTCAAAATAGAAAAAAAAAAACTGTGTCCTGTTT
TGCACATAGAAAGAAGCAGATCAGATTGTCCTATGTTGCGCTGTTATATATGAATGTTTGGACTGTACATCTAAAGAATGATTCCGTCCT
AACACCCACCCAGCCTGTTTGAACACATTCTAATTGTCTAACATTTGTTGCATTTTAAGTATGAGATGACTGATCTTATGAATGTTTTGT
AGAAAATGTTATAATTTAACAGTCTTTGCGAGTATACATGTTTTTTAACAAGTTGAAAATGTTTCATCTGAACCTTTCCTAACTTTTTTT
TGCCAGTTTGAGTTCAAATCAAATACACCTTAAGTTCTGGTTTCATGTTCTCTTGAAACATTGAATTGTACGTAAATATAAAGGATCTAC
TGTTGCGGAAAATTAAGAAGCTAAATACTGCTCAGTTATCTTTGCCTGCGTGTATATTTTTAAAAATTCACTCTGAAGTGTTATGGATGC
TTTTCTTTGAGTTCTAAATTTTGGTGGGAATTACTTTATGTCTCCCAGTTAAGAAGAGTCTCCATTTTCCAGAAAATACAGCTAAGCAAG
AAAAATATCTTTTAAATACTTTGCCTTTAAAACACTTTTTTATTTTGTTTTTCTATTTTAAAAATAAAATATTATTTTCTGAATTTCTTC
TATTGGGTAATCCAGTTAGACCATATTCCTCTACAAATAAAAACTATGTTGATTTCATAGTAAAGTAAGTTGTAATAGAGATAATTATTT
TTTTCTGTTGTTAGCTGCTTATGAAACTTTTATTGTATTTTTTTAAAGTTATGCTACTCCTGGCCGGGCATGGTGGCTCACGCCTGTAAT
CCCAGCACTTTGGGAGGCCGAGGCACGTGGATCACTTGATGTCAGGAGTTCGAGACCAGCCTGGCTAACATGGTGAAACCCTGTCTCCAG
TACAAATACAATAATTAGCTGGGTGTGGTGGCGCACACCTGTAATCCCAGCTACTTGGGAGGCTGAGGCAGGAGAATCACTTGAACCTGG
GAGGCGGAGGTTGCAGTGAGCTGAGATGGCATCACTGCACTCCAGCCTGGGTGACAGGGTGACACTCCACCTCAAAAAATAAAAATAAAA
TAAAAATAAAGTTATGCTACTCCTAAACCACTTAATATATGTTTTGTGGTTTTCAAAATTATGTAATCAGCCCTGAAACACATTGGTTGA
AAATATTTTGAGACTTTATAAGTTAGTATTTATTAAATTAATATTATTTTAATAAGTTTTGTTAAATCCTAGTTTAAATGACAAAGCTGT
GTATAGAGTAGGGGTGAGTGAAGGTGGAGTACTCTTGAGGTGGCGATAAAAATGTACCCATAAATTTAAAGTCCATTCTGTAATTGGAAT
ACTGAATGTCCTGTGTGTGGTGAACATTTTCTAGGTGATTAGAATTGCATTTCACTGGCTGCATTTGTTTGTGGTATGTAAGATAGCTAG
AAATTTATTACTTAACTCTGTGGGGCATTGTTTAGATACTGTGAAGAGTTGAGGTAAATATGTGTCTTGTAAAAGCTTTGTGTGATCAGA
ATATTTTGGTAATTATAAAATGTTTTTGATGATACACATTTAACTCTGTTAGTAATTACTCTAAAGAAATCTTAATTTTACCAGTTTAAA
AAAAACGCTTCTTTTTAAAAGTTCATGTTTCTAACCTTTAACAATTCAGTTAATAGGAAAGGAGTAACTTTTAAATCAAGCTTTAAAACA
TGGAAATTTAAGCCAGTATGTTAGATATTTTTTCTATCCCACCCTACCATGATAATAAAAATAAATCTATAAAAAGTCAAAATAAAAAGC
CGATCTATAAAAAGTCAATTGAGAAGTTCTAAAAGCAGGCTACTATAGCAGGGGTGTTTGTTTTTTGTTTGGAGACGGAGTCTGGCTCTA
TTACCTAGGCTGGAGTGCAGTGGGACAATCTCGGCTCACTGTAACCTCTGTCTCTTCAGCCTCCTGAGTTGCTGGAACTACAGCTGTATG
CCATCATGCTCGGCTAATTTTTGTATTTTTTTGTAGAGATGGGGTTTTGCCATGTTGCCCGGGCCTGTCTTGAACTCCTAGAGCTTAAGT
GATCCACCCGCCTCAGGCTCCCGAAGTGCTGGGATTACAGGCATGATTATAGGCCTGAGCCACCACACCCCGCCTACTATAGCAGTTTTT
TAAAAAAAGCATTATGTTAAAACTGATTATACCAGATGGACATTAAATATCCATCTCAGAAATACCAAAATTTGATACATAAGCCTGGCC
TGAATATCTTTTTTTTTTTTTTGAGACGGAGTCTTACTCTGTCGCCCAGGCTGGAGTGCAGTGGCGCGATCTCGGCTCACTGCAACCTCT
GCCTCCTGGGTTCAAGGGATTCTCCTGCCTCAGCCTCTTGAGTAGCTGGGATTACAGGTTTGCGCCACCACACCCAGCTAATTTTTGTAT
TTTTTTTTTAGTAGAGACCGGGTTTCACCATGTTGGCCAGGCTGGTCTCACCTGACTTTGTGATCCGCCCGCCTCAGCCTCCCAAAGTGC
TGGGATTACAGGTGTGAGCCACCACGCCTGGCCTCAAGCCTGGTCTGAATATCTATATGCAAATATGGGACACTGACTTCAGAGCTATTC
ATTTAGCTCCAGTCATAGCAATTCTGTAGTTTCCTCACATTTACTGTGTCTTTGTCCACAAATGAAATATGTTGTAAGGTCAGTGTTAAT
TTCGTGAGACGATATAGATGAACACACTTGCTCATTTTTTGAAATAAATATGCAGATTTAAAATGAGTGGCACATATATATAAATAGGTT
ATTGAATGGGTATTCTCTTTTCCCTTTAATTTAAAAAGTGATATTTATTTATTAAAAGGAGCATTGGTAGAATTATTGGTTTATAGTATC
TTCTTTAATAATCCTTACAATTACATATACTAATAGGATGTTAAAATTATCATCGTGTTTCCTATTTGTGTTTAGATTCTTATAAAGCAT
GAGATGAGTAATCAAGGGAAGGGTCGCATCTCCATGTTTGCCCCATGAATAGAATCCATTTGACAAGCCATTTCATTTCCTTTCTGGAGA
AAGGGAGTTGTTTTTTTTTTCTTTTTACAGCAAAAATTTAAGGTTTTATTGGGGGAGGGGGTGGTGCTACTTCCTCTGTAACATGTTTTT
AAAACTATGATTATGGTTTTTGGTTGTTTTTTGTTTTGTTTGTTTCCTTTTAGTCTGTTTTTAATATCCTTCCCCTTAACTCTTAAGGAA
AGTCTTCTTTCCTACAGGGCTGTGAATTAAGGAATGGGTAGCGTTTACATTTAAGTACACCAATGTCTGCCCAATTTCCTAATGTATACA
TTTGTTTTATTTTAAATTATTTTTTGTTCTTAAAAATACTAGAAGAAAACATAGATGTTTCTAGTGCCTTTGAACTTATACTGTAGTCTG
ACTAATTTAATGAATAACTTCAGTAGCATTACTATAATACTGTATGCTGGCCAAAATTACAAATTACAGTTTGTTGCATCAGTTTCAAAA
GCCATGCTTTCATTGTATTTTATGTATAAATTGTATTTGTTCAAGTTGTATATATTGGTATTGTATGTATACATGCAGCATGGTTAAATA

>In-frame_ENST00000351097_ENST00000378746_TCGA-L5-A8NE_USP25_chr21_17138460_+_KIF3A_chr5_132039311_length(amino acids)=439AA_start in transcript=189_stop in transcript=1508
MPAVPAHAGRHRLRAWLAGALSSQAVRAAPWSSAERGSQGRRRREEPGATAAAAAAAAGAMTVEQNVLQQSAAQKHQQTFLNQLREITGI
NDTQILQQALKDSNGNLELAVAFLTAKNAKTPQQEETTYYQTALPGNDRYISVGSQADTRKKKVSPDKMIEMQAKIDEERKALETKLDME
EEERNKARAELEKREKDLLKAQQEHQSLLEKLSALEKKVIVGGVDLLAKAEEQEKLLEESNMELEERRKRAEQLRRELEEKEQERLDIEE
KYTSLQEEAQGKTKKLKKVWTMLMAAKSEMADLQQEHQREIEGLLENIRQLSRELRLQMLIIDNFIPRDYQEMIENYVHWNEDIGEWQLK

--------------------------------------------------------------
>In-frame_ENST00000351097_ENST00000378735_TCGA-L5-A8NE_USP25_chr21_17138460_+_KIF3A_chr5_132039311_length(transcript)=2513nt_BP=637nt
AAGCAGCCCGCGGACCGGCAGCAAAGGAACGTGCGAACGCGTGACGCCGCCCGACTGGCTCGCGCTCTCCCGTGCCCCGGCGTCCTCCGC
CCGCTCATGGCCCGGGCCGCCGCGGACGAGCGGCGCTGAGGCGGGCCGCGTGGAGACGTGAGGCGGCCGCCGTGGCCCTCACAGTCGGCG
TTTCGCCGCCTGCCCGCGGTGCCCGCGCACGCCGGCCGCCATCGCCTTCGCGCCTGGCTGGCGGGGGCGCTGTCCTCCCAGGCCGTCCGC
GCCGCTCCCTGGAGCTCGGCGGAGCGCGGCAGCCAGGGCCGGCGGAGGCGCGAGGAGCCGGGCGCCACCGCCGCCGCCGCCGCCGCCGCC
GCGGGGGCCATGACCGTGGAGCAGAACGTGCTGCAGCAGAGCGCGGCGCAGAAGCACCAGCAGACGTTTTTGAATCAACTGAGAGAAATT
ACGGGGATTAATGACACCCAGATACTACAGCAAGCCTTGAAGGATAGTAATGGAAACTTGGAATTAGCAGTGGCTTTCCTTACTGCGAAG
AATGCTAAGACCCCTCAGCAGGAGGAGACAACTTACTACCAAACAGCACTTCCTGGCAATGATAGATACATCAGTGTGGGAAGCCAAGCA
GATACAAGAAAAAAGAAAGTCTCCCCAGACAAGATGATTGAAATGCAAGCAAAAATTGATGAGGAGAGAAAAGCACTTGAAACAAAGCTC
GACATGGAAGAAGAAGAAAGAAACAAGGCTAGAGCTGAATTAGAGAAACGGGAAAAAGATCTTCTTAAAGCCCAACAAGAGCATCAGTCT
TTGCTGGAAAAATTATCTGCCCTGGAAAAGAAGGTAATTGTTGGTGGGGTTGACTTGTTGGCCAAAGCTGAGGAACAAGAGAAACTTCTT
GAAGAATCTAACATGGAACTGGAAGAAAGGAGGAAAAGAGCAGAGCAACTTCGCAGAGAACTTGAGGAAAAAGAGCAAGAACGCTTGGAT
ATTGAAGAAAAATATACCAGTTTGCAAGAGGAAGCACAGGGAAAGACCAAGAAGTTAAAGAAAGTTTGGACTATGCTGATGGCTGCAAAG
TCAGAGATGGCTGATCTCCAACAAGAACATCAGAGGGAAATTGAAGGCCTACTGGAGAACATTCGGCAACTTAGCCGGGAGCTTCGACTT
CAGATGCTTATTATTGATAACTTTATACCTCGGGATTATCAGGAAATGATTGAAAACTATGTCCATTGGAATGAAGACATAGGAGAATGG
CAGCTAAAATGTGTTGCTTATACAGGAAATAACATGAGGAAGCAAACCCCAGTACCTGATAAAAAGGAGAAAGATCCCTTTGAGGTGGAC
CTTTCTCACGTGTATCTTGCCTATACTGAGGAGAGTCTGCGTCAGTCTTTGATGAAACTAGAAAGACCACGAACTTCAAAGGGGAAAGCA
AGGCCAAAGACAGGGAGAAGAAAGCGTTCTGCAAAGCCTGAAACTGTAATTGACTCTTTACTGCAGTAAATGTTACAGACTTAAAGTCAC
AATAAAAATTAGTGATATTCTCATGCCTGGACAAAATCTTTAATTAAAACAGTGAAGACTTCTCTATAATTTCAATTAATGGAAGTTGTA
GATCAATGAATAAGACTGGAAATAATAATTTGCTTAAGAACTTTTAGTCTACATATATTAATATAACATTAAAATTGTACAACTGGTGAT
TGTTCAAATTGTTGTACTACTACTCTTCAATTGTACTGTGCAGGGAAGTTGGGGGAACAGTTCATACTATAGAGAGTTACAGTTTAGATG
TATGTGTAAATCGATTAGCTATTTATCCAACTACTGTATATTTAGGTAACTAGAATTTCAAAATAGAAAAAAAAAAACTGTGTCCTGTTT
TGCACATAGAAAGAAGCAGATCAGATTGTCCTATGTTGCGCTGTTATATATGAATGTTTGGACTGTACATCTAAAGAATGATTCCGTCCT
AACACCCACCCAGCCTGTTTGAACACATTCTAATTGTCTAACATTTGTTGCATTTTAAGTATGAGATGACTGATCTTATGAATGTTTTGT
AGAAAATGTTATAATTTAACAGTCTTTGCGAGTATACATGTTTTTTAACAAGTTGAAAATGTTTCATCTGAACCTTTCCTAACTTTTTTT
TGCCAGTTTGAGTTCAAATCAAATACACCTTAAGTTCTGGTTTCATGTTCTCTTGAAACATTGAATTGTACGTAAATATAAAGGATCTAC
TGTTGCGGAAAATTAAGAAGCTAAATACTGCTCAGTTATCTTTGCCTGCGTGTATATTTTTAAAAATTCACTCTGAAGTGTTATGGATGC
TTTTCTTTGAGTTCTAAATTTTGGTGGGAATTACTTTATGTCTCCCAGTTAAGAAGAGTCTCCATTTTCCAGAAAATACAGCTAAGCAAG

>In-frame_ENST00000351097_ENST00000378735_TCGA-L5-A8NE_USP25_chr21_17138460_+_KIF3A_chr5_132039311_length(amino acids)=439AA_start in transcript=189_stop in transcript=1508
MPAVPAHAGRHRLRAWLAGALSSQAVRAAPWSSAERGSQGRRRREEPGATAAAAAAAAGAMTVEQNVLQQSAAQKHQQTFLNQLREITGI
NDTQILQQALKDSNGNLELAVAFLTAKNAKTPQQEETTYYQTALPGNDRYISVGSQADTRKKKVSPDKMIEMQAKIDEERKALETKLDME
EEERNKARAELEKREKDLLKAQQEHQSLLEKLSALEKKVIVGGVDLLAKAEEQEKLLEESNMELEERRKRAEQLRRELEEKEQERLDIEE
KYTSLQEEAQGKTKKLKKVWTMLMAAKSEMADLQQEHQREIEGLLENIRQLSRELRLQMLIIDNFIPRDYQEMIENYVHWNEDIGEWQLK

--------------------------------------------------------------
>In-frame_ENST00000351097_ENST00000403231_TCGA-L5-A8NE_USP25_chr21_17138460_+_KIF3A_chr5_132039311_length(transcript)=1509nt_BP=637nt
AAGCAGCCCGCGGACCGGCAGCAAAGGAACGTGCGAACGCGTGACGCCGCCCGACTGGCTCGCGCTCTCCCGTGCCCCGGCGTCCTCCGC
CCGCTCATGGCCCGGGCCGCCGCGGACGAGCGGCGCTGAGGCGGGCCGCGTGGAGACGTGAGGCGGCCGCCGTGGCCCTCACAGTCGGCG
TTTCGCCGCCTGCCCGCGGTGCCCGCGCACGCCGGCCGCCATCGCCTTCGCGCCTGGCTGGCGGGGGCGCTGTCCTCCCAGGCCGTCCGC
GCCGCTCCCTGGAGCTCGGCGGAGCGCGGCAGCCAGGGCCGGCGGAGGCGCGAGGAGCCGGGCGCCACCGCCGCCGCCGCCGCCGCCGCC
GCGGGGGCCATGACCGTGGAGCAGAACGTGCTGCAGCAGAGCGCGGCGCAGAAGCACCAGCAGACGTTTTTGAATCAACTGAGAGAAATT
ACGGGGATTAATGACACCCAGATACTACAGCAAGCCTTGAAGGATAGTAATGGAAACTTGGAATTAGCAGTGGCTTTCCTTACTGCGAAG
AATGCTAAGACCCCTCAGCAGGAGGAGACAACTTACTACCAAACAGCACTTCCTGGCAATGATAGATACATCAGTGTGGGAAGCCAAGCA
GATACAAGAAAAAAGAAAGTCTCCCCAGACAAGATGATTGAAATGCAAGCAAAAATTGATGAGGAGAGAAAAGCACTTGAAACAAAGCTC
GACATGGAAGAAGAAGAAAGAAACAAGGCTAGAGCTGAATTAGAGAAACGGGAAAAAGATCTTCTTAAAGCCCAACAAGAGCATCAGTCT
TTGCTGGAAAAATTATCTGCCCTGGAAAAGAAGGTAATTGTTGGTGGGGTTGACTTGTTGGCCAAAGCTGAGGAACAAGAGAAACTTCTT
GAAGAATCTAACATGGAACTGGAAGAAAGGAGGAAAAGAGCAGAGCAACTTCGCAGAGAACTTGAGGAAAAAGAGCAAGAACGCTTGGAT
ATTGAAGAAAAATATACCAGTTTGCAAGAGGAAGCACAGGGAAAGACCAAGAAGTTAAAGAAAGTTTGGACTATGCTGATGGCTGCAAAG
TCAGAGATGGCTGATCTCCAACAAGAACATCAGAGGGAAATTGAAGGCCTACTGGAGAACATTCGGCAACTTAGCCGGGAGCTTCGACTT
CAGATGCTTATTATTGATAACTTTATACCTCGGGATTATCAGGAAATGATTGAAAACTATGTCCATTGGAATGAAGACATAGGAGAATGG
CAGCTAAAATGTGTTGCTTATACAGGAAATAACATGAGGAAGCAAACCCCAGTACCTGATAAAAAGGAGAAAGATCCCTTTGAGGTGGAC
CTTTCTCACGTGTATCTTGCCTATACTGAGGAGAGTCTGCGTCAGTCTTTGATGAAACTAGAAAGACCACGAACTTCAAAGGGGAAAGCA

>In-frame_ENST00000351097_ENST00000403231_TCGA-L5-A8NE_USP25_chr21_17138460_+_KIF3A_chr5_132039311_length(amino acids)=439AA_start in transcript=189_stop in transcript=1508
MPAVPAHAGRHRLRAWLAGALSSQAVRAAPWSSAERGSQGRRRREEPGATAAAAAAAAGAMTVEQNVLQQSAAQKHQQTFLNQLREITGI
NDTQILQQALKDSNGNLELAVAFLTAKNAKTPQQEETTYYQTALPGNDRYISVGSQADTRKKKVSPDKMIEMQAKIDEERKALETKLDME
EEERNKARAELEKREKDLLKAQQEHQSLLEKLSALEKKVIVGGVDLLAKAEEQEKLLEESNMELEERRKRAEQLRRELEEKEQERLDIEE
KYTSLQEEAQGKTKKLKKVWTMLMAAKSEMADLQQEHQREIEGLLENIRQLSRELRLQMLIIDNFIPRDYQEMIENYVHWNEDIGEWQLK

--------------------------------------------------------------
>In-frame_ENST00000285679_ENST00000378746_TCGA-L5-A8NE_USP25_chr21_17138460_+_KIF3A_chr5_132039311_length(transcript)=5515nt_BP=637nt
AAGCAGCCCGCGGACCGGCAGCAAAGGAACGTGCGAACGCGTGACGCCGCCCGACTGGCTCGCGCTCTCCCGTGCCCCGGCGTCCTCCGC
CCGCTCATGGCCCGGGCCGCCGCGGACGAGCGGCGCTGAGGCGGGCCGCGTGGAGACGTGAGGCGGCCGCCGTGGCCCTCACAGTCGGCG
TTTCGCCGCCTGCCCGCGGTGCCCGCGCACGCCGGCCGCCATCGCCTTCGCGCCTGGCTGGCGGGGGCGCTGTCCTCCCAGGCCGTCCGC
GCCGCTCCCTGGAGCTCGGCGGAGCGCGGCAGCCAGGGCCGGCGGAGGCGCGAGGAGCCGGGCGCCACCGCCGCCGCCGCCGCCGCCGCC
GCGGGGGCCATGACCGTGGAGCAGAACGTGCTGCAGCAGAGCGCGGCGCAGAAGCACCAGCAGACGTTTTTGAATCAACTGAGAGAAATT
ACGGGGATTAATGACACCCAGATACTACAGCAAGCCTTGAAGGATAGTAATGGAAACTTGGAATTAGCAGTGGCTTTCCTTACTGCGAAG
AATGCTAAGACCCCTCAGCAGGAGGAGACAACTTACTACCAAACAGCACTTCCTGGCAATGATAGATACATCAGTGTGGGAAGCCAAGCA
GATACAAGAAAAAAGAAAGTCTCCCCAGACAAGATGATTGAAATGCAAGCAAAAATTGATGAGGAGAGAAAAGCACTTGAAACAAAGCTC
GACATGGAAGAAGAAGAAAGAAACAAGGCTAGAGCTGAATTAGAGAAACGGGAAAAAGATCTTCTTAAAGCCCAACAAGAGCATCAGTCT
TTGCTGGAAAAATTATCTGCCCTGGAAAAGAAGGTAATTGTTGGTGGGGTTGACTTGTTGGCCAAAGCTGAGGAACAAGAGAAACTTCTT
GAAGAATCTAACATGGAACTGGAAGAAAGGAGGAAAAGAGCAGAGCAACTTCGCAGAGAACTTGAGGAAAAAGAGCAAGAACGCTTGGAT
ATTGAAGAAAAATATACCAGTTTGCAAGAGGAAGCACAGGGAAAGACCAAGAAGTTAAAGAAAGTTTGGACTATGCTGATGGCTGCAAAG
TCAGAGATGGCTGATCTCCAACAAGAACATCAGAGGGAAATTGAAGGCCTACTGGAGAACATTCGGCAACTTAGCCGGGAGCTTCGACTT
CAGATGCTTATTATTGATAACTTTATACCTCGGGATTATCAGGAAATGATTGAAAACTATGTCCATTGGAATGAAGACATAGGAGAATGG
CAGCTAAAATGTGTTGCTTATACAGGAAATAACATGAGGAAGCAAACCCCAGTACCTGATAAAAAGGAGAAAGATCCCTTTGAGGTGGAC
CTTTCTCACGTGTATCTTGCCTATACTGAGGAGAGTCTGCGTCAGTCTTTGATGAAACTAGAAAGACCACGAACTTCAAAGGGGAAAGCA
AGGCCAAAGACAGGGAGAAGAAAGCGTTCTGCAAAGCCTGAAACTGTAATTGACTCTTTACTGCAGTAAATGTTACAGACTTAAAGTCAC
AATAAAAATTAGTGATATTCTCATGCCTGGACAAAATCTTTAATTAAAACAGTGAAGACTTCTCTATAATTTCAATTAATGGAAGTTGTA
GATCAATGAATAAGACTGGAAATAATAATTTGCTTAAGAACTTTTAGTCTACATATATTAATATAACATTAAAATTGTACAACTGGTGAT
TGTTCAAATTGTTGTACTACTACTCTTCAATTGTACTGTGCAGGGAAGTTGGGGGAACAGTTCATACTATAGAGAGTTACAGTTTAGATG
TATGTGTAAATCGATTAGCTATTTATCCAACTACTGTATATTTAGGTAACTAGAATTTCAAAATAGAAAAAAAAAAACTGTGTCCTGTTT
TGCACATAGAAAGAAGCAGATCAGATTGTCCTATGTTGCGCTGTTATATATGAATGTTTGGACTGTACATCTAAAGAATGATTCCGTCCT
AACACCCACCCAGCCTGTTTGAACACATTCTAATTGTCTAACATTTGTTGCATTTTAAGTATGAGATGACTGATCTTATGAATGTTTTGT
AGAAAATGTTATAATTTAACAGTCTTTGCGAGTATACATGTTTTTTAACAAGTTGAAAATGTTTCATCTGAACCTTTCCTAACTTTTTTT
TGCCAGTTTGAGTTCAAATCAAATACACCTTAAGTTCTGGTTTCATGTTCTCTTGAAACATTGAATTGTACGTAAATATAAAGGATCTAC
TGTTGCGGAAAATTAAGAAGCTAAATACTGCTCAGTTATCTTTGCCTGCGTGTATATTTTTAAAAATTCACTCTGAAGTGTTATGGATGC
TTTTCTTTGAGTTCTAAATTTTGGTGGGAATTACTTTATGTCTCCCAGTTAAGAAGAGTCTCCATTTTCCAGAAAATACAGCTAAGCAAG
AAAAATATCTTTTAAATACTTTGCCTTTAAAACACTTTTTTATTTTGTTTTTCTATTTTAAAAATAAAATATTATTTTCTGAATTTCTTC
TATTGGGTAATCCAGTTAGACCATATTCCTCTACAAATAAAAACTATGTTGATTTCATAGTAAAGTAAGTTGTAATAGAGATAATTATTT
TTTTCTGTTGTTAGCTGCTTATGAAACTTTTATTGTATTTTTTTAAAGTTATGCTACTCCTGGCCGGGCATGGTGGCTCACGCCTGTAAT
CCCAGCACTTTGGGAGGCCGAGGCACGTGGATCACTTGATGTCAGGAGTTCGAGACCAGCCTGGCTAACATGGTGAAACCCTGTCTCCAG
TACAAATACAATAATTAGCTGGGTGTGGTGGCGCACACCTGTAATCCCAGCTACTTGGGAGGCTGAGGCAGGAGAATCACTTGAACCTGG
GAGGCGGAGGTTGCAGTGAGCTGAGATGGCATCACTGCACTCCAGCCTGGGTGACAGGGTGACACTCCACCTCAAAAAATAAAAATAAAA
TAAAAATAAAGTTATGCTACTCCTAAACCACTTAATATATGTTTTGTGGTTTTCAAAATTATGTAATCAGCCCTGAAACACATTGGTTGA
AAATATTTTGAGACTTTATAAGTTAGTATTTATTAAATTAATATTATTTTAATAAGTTTTGTTAAATCCTAGTTTAAATGACAAAGCTGT
GTATAGAGTAGGGGTGAGTGAAGGTGGAGTACTCTTGAGGTGGCGATAAAAATGTACCCATAAATTTAAAGTCCATTCTGTAATTGGAAT
ACTGAATGTCCTGTGTGTGGTGAACATTTTCTAGGTGATTAGAATTGCATTTCACTGGCTGCATTTGTTTGTGGTATGTAAGATAGCTAG
AAATTTATTACTTAACTCTGTGGGGCATTGTTTAGATACTGTGAAGAGTTGAGGTAAATATGTGTCTTGTAAAAGCTTTGTGTGATCAGA
ATATTTTGGTAATTATAAAATGTTTTTGATGATACACATTTAACTCTGTTAGTAATTACTCTAAAGAAATCTTAATTTTACCAGTTTAAA
AAAAACGCTTCTTTTTAAAAGTTCATGTTTCTAACCTTTAACAATTCAGTTAATAGGAAAGGAGTAACTTTTAAATCAAGCTTTAAAACA
TGGAAATTTAAGCCAGTATGTTAGATATTTTTTCTATCCCACCCTACCATGATAATAAAAATAAATCTATAAAAAGTCAAAATAAAAAGC
CGATCTATAAAAAGTCAATTGAGAAGTTCTAAAAGCAGGCTACTATAGCAGGGGTGTTTGTTTTTTGTTTGGAGACGGAGTCTGGCTCTA
TTACCTAGGCTGGAGTGCAGTGGGACAATCTCGGCTCACTGTAACCTCTGTCTCTTCAGCCTCCTGAGTTGCTGGAACTACAGCTGTATG
CCATCATGCTCGGCTAATTTTTGTATTTTTTTGTAGAGATGGGGTTTTGCCATGTTGCCCGGGCCTGTCTTGAACTCCTAGAGCTTAAGT
GATCCACCCGCCTCAGGCTCCCGAAGTGCTGGGATTACAGGCATGATTATAGGCCTGAGCCACCACACCCCGCCTACTATAGCAGTTTTT
TAAAAAAAGCATTATGTTAAAACTGATTATACCAGATGGACATTAAATATCCATCTCAGAAATACCAAAATTTGATACATAAGCCTGGCC
TGAATATCTTTTTTTTTTTTTTGAGACGGAGTCTTACTCTGTCGCCCAGGCTGGAGTGCAGTGGCGCGATCTCGGCTCACTGCAACCTCT
GCCTCCTGGGTTCAAGGGATTCTCCTGCCTCAGCCTCTTGAGTAGCTGGGATTACAGGTTTGCGCCACCACACCCAGCTAATTTTTGTAT
TTTTTTTTTAGTAGAGACCGGGTTTCACCATGTTGGCCAGGCTGGTCTCACCTGACTTTGTGATCCGCCCGCCTCAGCCTCCCAAAGTGC
TGGGATTACAGGTGTGAGCCACCACGCCTGGCCTCAAGCCTGGTCTGAATATCTATATGCAAATATGGGACACTGACTTCAGAGCTATTC
ATTTAGCTCCAGTCATAGCAATTCTGTAGTTTCCTCACATTTACTGTGTCTTTGTCCACAAATGAAATATGTTGTAAGGTCAGTGTTAAT
TTCGTGAGACGATATAGATGAACACACTTGCTCATTTTTTGAAATAAATATGCAGATTTAAAATGAGTGGCACATATATATAAATAGGTT
ATTGAATGGGTATTCTCTTTTCCCTTTAATTTAAAAAGTGATATTTATTTATTAAAAGGAGCATTGGTAGAATTATTGGTTTATAGTATC
TTCTTTAATAATCCTTACAATTACATATACTAATAGGATGTTAAAATTATCATCGTGTTTCCTATTTGTGTTTAGATTCTTATAAAGCAT
GAGATGAGTAATCAAGGGAAGGGTCGCATCTCCATGTTTGCCCCATGAATAGAATCCATTTGACAAGCCATTTCATTTCCTTTCTGGAGA
AAGGGAGTTGTTTTTTTTTTCTTTTTACAGCAAAAATTTAAGGTTTTATTGGGGGAGGGGGTGGTGCTACTTCCTCTGTAACATGTTTTT
AAAACTATGATTATGGTTTTTGGTTGTTTTTTGTTTTGTTTGTTTCCTTTTAGTCTGTTTTTAATATCCTTCCCCTTAACTCTTAAGGAA
AGTCTTCTTTCCTACAGGGCTGTGAATTAAGGAATGGGTAGCGTTTACATTTAAGTACACCAATGTCTGCCCAATTTCCTAATGTATACA
TTTGTTTTATTTTAAATTATTTTTTGTTCTTAAAAATACTAGAAGAAAACATAGATGTTTCTAGTGCCTTTGAACTTATACTGTAGTCTG
ACTAATTTAATGAATAACTTCAGTAGCATTACTATAATACTGTATGCTGGCCAAAATTACAAATTACAGTTTGTTGCATCAGTTTCAAAA
GCCATGCTTTCATTGTATTTTATGTATAAATTGTATTTGTTCAAGTTGTATATATTGGTATTGTATGTATACATGCAGCATGGTTAAATA

>In-frame_ENST00000285679_ENST00000378746_TCGA-L5-A8NE_USP25_chr21_17138460_+_KIF3A_chr5_132039311_length(amino acids)=439AA_start in transcript=189_stop in transcript=1508
MPAVPAHAGRHRLRAWLAGALSSQAVRAAPWSSAERGSQGRRRREEPGATAAAAAAAAGAMTVEQNVLQQSAAQKHQQTFLNQLREITGI
NDTQILQQALKDSNGNLELAVAFLTAKNAKTPQQEETTYYQTALPGNDRYISVGSQADTRKKKVSPDKMIEMQAKIDEERKALETKLDME
EEERNKARAELEKREKDLLKAQQEHQSLLEKLSALEKKVIVGGVDLLAKAEEQEKLLEESNMELEERRKRAEQLRRELEEKEQERLDIEE
KYTSLQEEAQGKTKKLKKVWTMLMAAKSEMADLQQEHQREIEGLLENIRQLSRELRLQMLIIDNFIPRDYQEMIENYVHWNEDIGEWQLK

--------------------------------------------------------------
>In-frame_ENST00000285679_ENST00000378735_TCGA-L5-A8NE_USP25_chr21_17138460_+_KIF3A_chr5_132039311_length(transcript)=2513nt_BP=637nt
AAGCAGCCCGCGGACCGGCAGCAAAGGAACGTGCGAACGCGTGACGCCGCCCGACTGGCTCGCGCTCTCCCGTGCCCCGGCGTCCTCCGC
CCGCTCATGGCCCGGGCCGCCGCGGACGAGCGGCGCTGAGGCGGGCCGCGTGGAGACGTGAGGCGGCCGCCGTGGCCCTCACAGTCGGCG
TTTCGCCGCCTGCCCGCGGTGCCCGCGCACGCCGGCCGCCATCGCCTTCGCGCCTGGCTGGCGGGGGCGCTGTCCTCCCAGGCCGTCCGC
GCCGCTCCCTGGAGCTCGGCGGAGCGCGGCAGCCAGGGCCGGCGGAGGCGCGAGGAGCCGGGCGCCACCGCCGCCGCCGCCGCCGCCGCC
GCGGGGGCCATGACCGTGGAGCAGAACGTGCTGCAGCAGAGCGCGGCGCAGAAGCACCAGCAGACGTTTTTGAATCAACTGAGAGAAATT
ACGGGGATTAATGACACCCAGATACTACAGCAAGCCTTGAAGGATAGTAATGGAAACTTGGAATTAGCAGTGGCTTTCCTTACTGCGAAG
AATGCTAAGACCCCTCAGCAGGAGGAGACAACTTACTACCAAACAGCACTTCCTGGCAATGATAGATACATCAGTGTGGGAAGCCAAGCA
GATACAAGAAAAAAGAAAGTCTCCCCAGACAAGATGATTGAAATGCAAGCAAAAATTGATGAGGAGAGAAAAGCACTTGAAACAAAGCTC
GACATGGAAGAAGAAGAAAGAAACAAGGCTAGAGCTGAATTAGAGAAACGGGAAAAAGATCTTCTTAAAGCCCAACAAGAGCATCAGTCT
TTGCTGGAAAAATTATCTGCCCTGGAAAAGAAGGTAATTGTTGGTGGGGTTGACTTGTTGGCCAAAGCTGAGGAACAAGAGAAACTTCTT
GAAGAATCTAACATGGAACTGGAAGAAAGGAGGAAAAGAGCAGAGCAACTTCGCAGAGAACTTGAGGAAAAAGAGCAAGAACGCTTGGAT
ATTGAAGAAAAATATACCAGTTTGCAAGAGGAAGCACAGGGAAAGACCAAGAAGTTAAAGAAAGTTTGGACTATGCTGATGGCTGCAAAG
TCAGAGATGGCTGATCTCCAACAAGAACATCAGAGGGAAATTGAAGGCCTACTGGAGAACATTCGGCAACTTAGCCGGGAGCTTCGACTT
CAGATGCTTATTATTGATAACTTTATACCTCGGGATTATCAGGAAATGATTGAAAACTATGTCCATTGGAATGAAGACATAGGAGAATGG
CAGCTAAAATGTGTTGCTTATACAGGAAATAACATGAGGAAGCAAACCCCAGTACCTGATAAAAAGGAGAAAGATCCCTTTGAGGTGGAC
CTTTCTCACGTGTATCTTGCCTATACTGAGGAGAGTCTGCGTCAGTCTTTGATGAAACTAGAAAGACCACGAACTTCAAAGGGGAAAGCA
AGGCCAAAGACAGGGAGAAGAAAGCGTTCTGCAAAGCCTGAAACTGTAATTGACTCTTTACTGCAGTAAATGTTACAGACTTAAAGTCAC
AATAAAAATTAGTGATATTCTCATGCCTGGACAAAATCTTTAATTAAAACAGTGAAGACTTCTCTATAATTTCAATTAATGGAAGTTGTA
GATCAATGAATAAGACTGGAAATAATAATTTGCTTAAGAACTTTTAGTCTACATATATTAATATAACATTAAAATTGTACAACTGGTGAT
TGTTCAAATTGTTGTACTACTACTCTTCAATTGTACTGTGCAGGGAAGTTGGGGGAACAGTTCATACTATAGAGAGTTACAGTTTAGATG
TATGTGTAAATCGATTAGCTATTTATCCAACTACTGTATATTTAGGTAACTAGAATTTCAAAATAGAAAAAAAAAAACTGTGTCCTGTTT
TGCACATAGAAAGAAGCAGATCAGATTGTCCTATGTTGCGCTGTTATATATGAATGTTTGGACTGTACATCTAAAGAATGATTCCGTCCT
AACACCCACCCAGCCTGTTTGAACACATTCTAATTGTCTAACATTTGTTGCATTTTAAGTATGAGATGACTGATCTTATGAATGTTTTGT
AGAAAATGTTATAATTTAACAGTCTTTGCGAGTATACATGTTTTTTAACAAGTTGAAAATGTTTCATCTGAACCTTTCCTAACTTTTTTT
TGCCAGTTTGAGTTCAAATCAAATACACCTTAAGTTCTGGTTTCATGTTCTCTTGAAACATTGAATTGTACGTAAATATAAAGGATCTAC
TGTTGCGGAAAATTAAGAAGCTAAATACTGCTCAGTTATCTTTGCCTGCGTGTATATTTTTAAAAATTCACTCTGAAGTGTTATGGATGC
TTTTCTTTGAGTTCTAAATTTTGGTGGGAATTACTTTATGTCTCCCAGTTAAGAAGAGTCTCCATTTTCCAGAAAATACAGCTAAGCAAG

>In-frame_ENST00000285679_ENST00000378735_TCGA-L5-A8NE_USP25_chr21_17138460_+_KIF3A_chr5_132039311_length(amino acids)=439AA_start in transcript=189_stop in transcript=1508
MPAVPAHAGRHRLRAWLAGALSSQAVRAAPWSSAERGSQGRRRREEPGATAAAAAAAAGAMTVEQNVLQQSAAQKHQQTFLNQLREITGI
NDTQILQQALKDSNGNLELAVAFLTAKNAKTPQQEETTYYQTALPGNDRYISVGSQADTRKKKVSPDKMIEMQAKIDEERKALETKLDME
EEERNKARAELEKREKDLLKAQQEHQSLLEKLSALEKKVIVGGVDLLAKAEEQEKLLEESNMELEERRKRAEQLRRELEEKEQERLDIEE
KYTSLQEEAQGKTKKLKKVWTMLMAAKSEMADLQQEHQREIEGLLENIRQLSRELRLQMLIIDNFIPRDYQEMIENYVHWNEDIGEWQLK

--------------------------------------------------------------
>In-frame_ENST00000285679_ENST00000403231_TCGA-L5-A8NE_USP25_chr21_17138460_+_KIF3A_chr5_132039311_length(transcript)=1509nt_BP=637nt
AAGCAGCCCGCGGACCGGCAGCAAAGGAACGTGCGAACGCGTGACGCCGCCCGACTGGCTCGCGCTCTCCCGTGCCCCGGCGTCCTCCGC
CCGCTCATGGCCCGGGCCGCCGCGGACGAGCGGCGCTGAGGCGGGCCGCGTGGAGACGTGAGGCGGCCGCCGTGGCCCTCACAGTCGGCG
TTTCGCCGCCTGCCCGCGGTGCCCGCGCACGCCGGCCGCCATCGCCTTCGCGCCTGGCTGGCGGGGGCGCTGTCCTCCCAGGCCGTCCGC
GCCGCTCCCTGGAGCTCGGCGGAGCGCGGCAGCCAGGGCCGGCGGAGGCGCGAGGAGCCGGGCGCCACCGCCGCCGCCGCCGCCGCCGCC
GCGGGGGCCATGACCGTGGAGCAGAACGTGCTGCAGCAGAGCGCGGCGCAGAAGCACCAGCAGACGTTTTTGAATCAACTGAGAGAAATT
ACGGGGATTAATGACACCCAGATACTACAGCAAGCCTTGAAGGATAGTAATGGAAACTTGGAATTAGCAGTGGCTTTCCTTACTGCGAAG
AATGCTAAGACCCCTCAGCAGGAGGAGACAACTTACTACCAAACAGCACTTCCTGGCAATGATAGATACATCAGTGTGGGAAGCCAAGCA
GATACAAGAAAAAAGAAAGTCTCCCCAGACAAGATGATTGAAATGCAAGCAAAAATTGATGAGGAGAGAAAAGCACTTGAAACAAAGCTC
GACATGGAAGAAGAAGAAAGAAACAAGGCTAGAGCTGAATTAGAGAAACGGGAAAAAGATCTTCTTAAAGCCCAACAAGAGCATCAGTCT
TTGCTGGAAAAATTATCTGCCCTGGAAAAGAAGGTAATTGTTGGTGGGGTTGACTTGTTGGCCAAAGCTGAGGAACAAGAGAAACTTCTT
GAAGAATCTAACATGGAACTGGAAGAAAGGAGGAAAAGAGCAGAGCAACTTCGCAGAGAACTTGAGGAAAAAGAGCAAGAACGCTTGGAT
ATTGAAGAAAAATATACCAGTTTGCAAGAGGAAGCACAGGGAAAGACCAAGAAGTTAAAGAAAGTTTGGACTATGCTGATGGCTGCAAAG
TCAGAGATGGCTGATCTCCAACAAGAACATCAGAGGGAAATTGAAGGCCTACTGGAGAACATTCGGCAACTTAGCCGGGAGCTTCGACTT
CAGATGCTTATTATTGATAACTTTATACCTCGGGATTATCAGGAAATGATTGAAAACTATGTCCATTGGAATGAAGACATAGGAGAATGG
CAGCTAAAATGTGTTGCTTATACAGGAAATAACATGAGGAAGCAAACCCCAGTACCTGATAAAAAGGAGAAAGATCCCTTTGAGGTGGAC
CTTTCTCACGTGTATCTTGCCTATACTGAGGAGAGTCTGCGTCAGTCTTTGATGAAACTAGAAAGACCACGAACTTCAAAGGGGAAAGCA

>In-frame_ENST00000285679_ENST00000403231_TCGA-L5-A8NE_USP25_chr21_17138460_+_KIF3A_chr5_132039311_length(amino acids)=439AA_start in transcript=189_stop in transcript=1508
MPAVPAHAGRHRLRAWLAGALSSQAVRAAPWSSAERGSQGRRRREEPGATAAAAAAAAGAMTVEQNVLQQSAAQKHQQTFLNQLREITGI
NDTQILQQALKDSNGNLELAVAFLTAKNAKTPQQEETTYYQTALPGNDRYISVGSQADTRKKKVSPDKMIEMQAKIDEERKALETKLDME
EEERNKARAELEKREKDLLKAQQEHQSLLEKLSALEKKVIVGGVDLLAKAEEQEKLLEESNMELEERRKRAEQLRRELEEKEQERLDIEE
KYTSLQEEAQGKTKKLKKVWTMLMAAKSEMADLQQEHQREIEGLLENIRQLSRELRLQMLIIDNFIPRDYQEMIENYVHWNEDIGEWQLK

--------------------------------------------------------------
>In-frame_ENST00000285681_ENST00000378746_TCGA-L5-A8NE_USP25_chr21_17138460_+_KIF3A_chr5_132039311_length(transcript)=5515nt_BP=637nt
AAGCAGCCCGCGGACCGGCAGCAAAGGAACGTGCGAACGCGTGACGCCGCCCGACTGGCTCGCGCTCTCCCGTGCCCCGGCGTCCTCCGC
CCGCTCATGGCCCGGGCCGCCGCGGACGAGCGGCGCTGAGGCGGGCCGCGTGGAGACGTGAGGCGGCCGCCGTGGCCCTCACAGTCGGCG
TTTCGCCGCCTGCCCGCGGTGCCCGCGCACGCCGGCCGCCATCGCCTTCGCGCCTGGCTGGCGGGGGCGCTGTCCTCCCAGGCCGTCCGC
GCCGCTCCCTGGAGCTCGGCGGAGCGCGGCAGCCAGGGCCGGCGGAGGCGCGAGGAGCCGGGCGCCACCGCCGCCGCCGCCGCCGCCGCC
GCGGGGGCCATGACCGTGGAGCAGAACGTGCTGCAGCAGAGCGCGGCGCAGAAGCACCAGCAGACGTTTTTGAATCAACTGAGAGAAATT
ACGGGGATTAATGACACCCAGATACTACAGCAAGCCTTGAAGGATAGTAATGGAAACTTGGAATTAGCAGTGGCTTTCCTTACTGCGAAG
AATGCTAAGACCCCTCAGCAGGAGGAGACAACTTACTACCAAACAGCACTTCCTGGCAATGATAGATACATCAGTGTGGGAAGCCAAGCA
GATACAAGAAAAAAGAAAGTCTCCCCAGACAAGATGATTGAAATGCAAGCAAAAATTGATGAGGAGAGAAAAGCACTTGAAACAAAGCTC
GACATGGAAGAAGAAGAAAGAAACAAGGCTAGAGCTGAATTAGAGAAACGGGAAAAAGATCTTCTTAAAGCCCAACAAGAGCATCAGTCT
TTGCTGGAAAAATTATCTGCCCTGGAAAAGAAGGTAATTGTTGGTGGGGTTGACTTGTTGGCCAAAGCTGAGGAACAAGAGAAACTTCTT
GAAGAATCTAACATGGAACTGGAAGAAAGGAGGAAAAGAGCAGAGCAACTTCGCAGAGAACTTGAGGAAAAAGAGCAAGAACGCTTGGAT
ATTGAAGAAAAATATACCAGTTTGCAAGAGGAAGCACAGGGAAAGACCAAGAAGTTAAAGAAAGTTTGGACTATGCTGATGGCTGCAAAG
TCAGAGATGGCTGATCTCCAACAAGAACATCAGAGGGAAATTGAAGGCCTACTGGAGAACATTCGGCAACTTAGCCGGGAGCTTCGACTT
CAGATGCTTATTATTGATAACTTTATACCTCGGGATTATCAGGAAATGATTGAAAACTATGTCCATTGGAATGAAGACATAGGAGAATGG
CAGCTAAAATGTGTTGCTTATACAGGAAATAACATGAGGAAGCAAACCCCAGTACCTGATAAAAAGGAGAAAGATCCCTTTGAGGTGGAC
CTTTCTCACGTGTATCTTGCCTATACTGAGGAGAGTCTGCGTCAGTCTTTGATGAAACTAGAAAGACCACGAACTTCAAAGGGGAAAGCA
AGGCCAAAGACAGGGAGAAGAAAGCGTTCTGCAAAGCCTGAAACTGTAATTGACTCTTTACTGCAGTAAATGTTACAGACTTAAAGTCAC
AATAAAAATTAGTGATATTCTCATGCCTGGACAAAATCTTTAATTAAAACAGTGAAGACTTCTCTATAATTTCAATTAATGGAAGTTGTA
GATCAATGAATAAGACTGGAAATAATAATTTGCTTAAGAACTTTTAGTCTACATATATTAATATAACATTAAAATTGTACAACTGGTGAT
TGTTCAAATTGTTGTACTACTACTCTTCAATTGTACTGTGCAGGGAAGTTGGGGGAACAGTTCATACTATAGAGAGTTACAGTTTAGATG
TATGTGTAAATCGATTAGCTATTTATCCAACTACTGTATATTTAGGTAACTAGAATTTCAAAATAGAAAAAAAAAAACTGTGTCCTGTTT
TGCACATAGAAAGAAGCAGATCAGATTGTCCTATGTTGCGCTGTTATATATGAATGTTTGGACTGTACATCTAAAGAATGATTCCGTCCT
AACACCCACCCAGCCTGTTTGAACACATTCTAATTGTCTAACATTTGTTGCATTTTAAGTATGAGATGACTGATCTTATGAATGTTTTGT
AGAAAATGTTATAATTTAACAGTCTTTGCGAGTATACATGTTTTTTAACAAGTTGAAAATGTTTCATCTGAACCTTTCCTAACTTTTTTT
TGCCAGTTTGAGTTCAAATCAAATACACCTTAAGTTCTGGTTTCATGTTCTCTTGAAACATTGAATTGTACGTAAATATAAAGGATCTAC
TGTTGCGGAAAATTAAGAAGCTAAATACTGCTCAGTTATCTTTGCCTGCGTGTATATTTTTAAAAATTCACTCTGAAGTGTTATGGATGC
TTTTCTTTGAGTTCTAAATTTTGGTGGGAATTACTTTATGTCTCCCAGTTAAGAAGAGTCTCCATTTTCCAGAAAATACAGCTAAGCAAG
AAAAATATCTTTTAAATACTTTGCCTTTAAAACACTTTTTTATTTTGTTTTTCTATTTTAAAAATAAAATATTATTTTCTGAATTTCTTC
TATTGGGTAATCCAGTTAGACCATATTCCTCTACAAATAAAAACTATGTTGATTTCATAGTAAAGTAAGTTGTAATAGAGATAATTATTT
TTTTCTGTTGTTAGCTGCTTATGAAACTTTTATTGTATTTTTTTAAAGTTATGCTACTCCTGGCCGGGCATGGTGGCTCACGCCTGTAAT
CCCAGCACTTTGGGAGGCCGAGGCACGTGGATCACTTGATGTCAGGAGTTCGAGACCAGCCTGGCTAACATGGTGAAACCCTGTCTCCAG
TACAAATACAATAATTAGCTGGGTGTGGTGGCGCACACCTGTAATCCCAGCTACTTGGGAGGCTGAGGCAGGAGAATCACTTGAACCTGG
GAGGCGGAGGTTGCAGTGAGCTGAGATGGCATCACTGCACTCCAGCCTGGGTGACAGGGTGACACTCCACCTCAAAAAATAAAAATAAAA
TAAAAATAAAGTTATGCTACTCCTAAACCACTTAATATATGTTTTGTGGTTTTCAAAATTATGTAATCAGCCCTGAAACACATTGGTTGA
AAATATTTTGAGACTTTATAAGTTAGTATTTATTAAATTAATATTATTTTAATAAGTTTTGTTAAATCCTAGTTTAAATGACAAAGCTGT
GTATAGAGTAGGGGTGAGTGAAGGTGGAGTACTCTTGAGGTGGCGATAAAAATGTACCCATAAATTTAAAGTCCATTCTGTAATTGGAAT
ACTGAATGTCCTGTGTGTGGTGAACATTTTCTAGGTGATTAGAATTGCATTTCACTGGCTGCATTTGTTTGTGGTATGTAAGATAGCTAG
AAATTTATTACTTAACTCTGTGGGGCATTGTTTAGATACTGTGAAGAGTTGAGGTAAATATGTGTCTTGTAAAAGCTTTGTGTGATCAGA
ATATTTTGGTAATTATAAAATGTTTTTGATGATACACATTTAACTCTGTTAGTAATTACTCTAAAGAAATCTTAATTTTACCAGTTTAAA
AAAAACGCTTCTTTTTAAAAGTTCATGTTTCTAACCTTTAACAATTCAGTTAATAGGAAAGGAGTAACTTTTAAATCAAGCTTTAAAACA
TGGAAATTTAAGCCAGTATGTTAGATATTTTTTCTATCCCACCCTACCATGATAATAAAAATAAATCTATAAAAAGTCAAAATAAAAAGC
CGATCTATAAAAAGTCAATTGAGAAGTTCTAAAAGCAGGCTACTATAGCAGGGGTGTTTGTTTTTTGTTTGGAGACGGAGTCTGGCTCTA
TTACCTAGGCTGGAGTGCAGTGGGACAATCTCGGCTCACTGTAACCTCTGTCTCTTCAGCCTCCTGAGTTGCTGGAACTACAGCTGTATG
CCATCATGCTCGGCTAATTTTTGTATTTTTTTGTAGAGATGGGGTTTTGCCATGTTGCCCGGGCCTGTCTTGAACTCCTAGAGCTTAAGT
GATCCACCCGCCTCAGGCTCCCGAAGTGCTGGGATTACAGGCATGATTATAGGCCTGAGCCACCACACCCCGCCTACTATAGCAGTTTTT
TAAAAAAAGCATTATGTTAAAACTGATTATACCAGATGGACATTAAATATCCATCTCAGAAATACCAAAATTTGATACATAAGCCTGGCC
TGAATATCTTTTTTTTTTTTTTGAGACGGAGTCTTACTCTGTCGCCCAGGCTGGAGTGCAGTGGCGCGATCTCGGCTCACTGCAACCTCT
GCCTCCTGGGTTCAAGGGATTCTCCTGCCTCAGCCTCTTGAGTAGCTGGGATTACAGGTTTGCGCCACCACACCCAGCTAATTTTTGTAT
TTTTTTTTTAGTAGAGACCGGGTTTCACCATGTTGGCCAGGCTGGTCTCACCTGACTTTGTGATCCGCCCGCCTCAGCCTCCCAAAGTGC
TGGGATTACAGGTGTGAGCCACCACGCCTGGCCTCAAGCCTGGTCTGAATATCTATATGCAAATATGGGACACTGACTTCAGAGCTATTC
ATTTAGCTCCAGTCATAGCAATTCTGTAGTTTCCTCACATTTACTGTGTCTTTGTCCACAAATGAAATATGTTGTAAGGTCAGTGTTAAT
TTCGTGAGACGATATAGATGAACACACTTGCTCATTTTTTGAAATAAATATGCAGATTTAAAATGAGTGGCACATATATATAAATAGGTT
ATTGAATGGGTATTCTCTTTTCCCTTTAATTTAAAAAGTGATATTTATTTATTAAAAGGAGCATTGGTAGAATTATTGGTTTATAGTATC
TTCTTTAATAATCCTTACAATTACATATACTAATAGGATGTTAAAATTATCATCGTGTTTCCTATTTGTGTTTAGATTCTTATAAAGCAT
GAGATGAGTAATCAAGGGAAGGGTCGCATCTCCATGTTTGCCCCATGAATAGAATCCATTTGACAAGCCATTTCATTTCCTTTCTGGAGA
AAGGGAGTTGTTTTTTTTTTCTTTTTACAGCAAAAATTTAAGGTTTTATTGGGGGAGGGGGTGGTGCTACTTCCTCTGTAACATGTTTTT
AAAACTATGATTATGGTTTTTGGTTGTTTTTTGTTTTGTTTGTTTCCTTTTAGTCTGTTTTTAATATCCTTCCCCTTAACTCTTAAGGAA
AGTCTTCTTTCCTACAGGGCTGTGAATTAAGGAATGGGTAGCGTTTACATTTAAGTACACCAATGTCTGCCCAATTTCCTAATGTATACA
TTTGTTTTATTTTAAATTATTTTTTGTTCTTAAAAATACTAGAAGAAAACATAGATGTTTCTAGTGCCTTTGAACTTATACTGTAGTCTG
ACTAATTTAATGAATAACTTCAGTAGCATTACTATAATACTGTATGCTGGCCAAAATTACAAATTACAGTTTGTTGCATCAGTTTCAAAA
GCCATGCTTTCATTGTATTTTATGTATAAATTGTATTTGTTCAAGTTGTATATATTGGTATTGTATGTATACATGCAGCATGGTTAAATA

>In-frame_ENST00000285681_ENST00000378746_TCGA-L5-A8NE_USP25_chr21_17138460_+_KIF3A_chr5_132039311_length(amino acids)=439AA_start in transcript=189_stop in transcript=1508
MPAVPAHAGRHRLRAWLAGALSSQAVRAAPWSSAERGSQGRRRREEPGATAAAAAAAAGAMTVEQNVLQQSAAQKHQQTFLNQLREITGI
NDTQILQQALKDSNGNLELAVAFLTAKNAKTPQQEETTYYQTALPGNDRYISVGSQADTRKKKVSPDKMIEMQAKIDEERKALETKLDME
EEERNKARAELEKREKDLLKAQQEHQSLLEKLSALEKKVIVGGVDLLAKAEEQEKLLEESNMELEERRKRAEQLRRELEEKEQERLDIEE
KYTSLQEEAQGKTKKLKKVWTMLMAAKSEMADLQQEHQREIEGLLENIRQLSRELRLQMLIIDNFIPRDYQEMIENYVHWNEDIGEWQLK

--------------------------------------------------------------
>In-frame_ENST00000285681_ENST00000378735_TCGA-L5-A8NE_USP25_chr21_17138460_+_KIF3A_chr5_132039311_length(transcript)=2513nt_BP=637nt
AAGCAGCCCGCGGACCGGCAGCAAAGGAACGTGCGAACGCGTGACGCCGCCCGACTGGCTCGCGCTCTCCCGTGCCCCGGCGTCCTCCGC
CCGCTCATGGCCCGGGCCGCCGCGGACGAGCGGCGCTGAGGCGGGCCGCGTGGAGACGTGAGGCGGCCGCCGTGGCCCTCACAGTCGGCG
TTTCGCCGCCTGCCCGCGGTGCCCGCGCACGCCGGCCGCCATCGCCTTCGCGCCTGGCTGGCGGGGGCGCTGTCCTCCCAGGCCGTCCGC
GCCGCTCCCTGGAGCTCGGCGGAGCGCGGCAGCCAGGGCCGGCGGAGGCGCGAGGAGCCGGGCGCCACCGCCGCCGCCGCCGCCGCCGCC
GCGGGGGCCATGACCGTGGAGCAGAACGTGCTGCAGCAGAGCGCGGCGCAGAAGCACCAGCAGACGTTTTTGAATCAACTGAGAGAAATT
ACGGGGATTAATGACACCCAGATACTACAGCAAGCCTTGAAGGATAGTAATGGAAACTTGGAATTAGCAGTGGCTTTCCTTACTGCGAAG
AATGCTAAGACCCCTCAGCAGGAGGAGACAACTTACTACCAAACAGCACTTCCTGGCAATGATAGATACATCAGTGTGGGAAGCCAAGCA
GATACAAGAAAAAAGAAAGTCTCCCCAGACAAGATGATTGAAATGCAAGCAAAAATTGATGAGGAGAGAAAAGCACTTGAAACAAAGCTC
GACATGGAAGAAGAAGAAAGAAACAAGGCTAGAGCTGAATTAGAGAAACGGGAAAAAGATCTTCTTAAAGCCCAACAAGAGCATCAGTCT
TTGCTGGAAAAATTATCTGCCCTGGAAAAGAAGGTAATTGTTGGTGGGGTTGACTTGTTGGCCAAAGCTGAGGAACAAGAGAAACTTCTT
GAAGAATCTAACATGGAACTGGAAGAAAGGAGGAAAAGAGCAGAGCAACTTCGCAGAGAACTTGAGGAAAAAGAGCAAGAACGCTTGGAT
ATTGAAGAAAAATATACCAGTTTGCAAGAGGAAGCACAGGGAAAGACCAAGAAGTTAAAGAAAGTTTGGACTATGCTGATGGCTGCAAAG
TCAGAGATGGCTGATCTCCAACAAGAACATCAGAGGGAAATTGAAGGCCTACTGGAGAACATTCGGCAACTTAGCCGGGAGCTTCGACTT
CAGATGCTTATTATTGATAACTTTATACCTCGGGATTATCAGGAAATGATTGAAAACTATGTCCATTGGAATGAAGACATAGGAGAATGG
CAGCTAAAATGTGTTGCTTATACAGGAAATAACATGAGGAAGCAAACCCCAGTACCTGATAAAAAGGAGAAAGATCCCTTTGAGGTGGAC
CTTTCTCACGTGTATCTTGCCTATACTGAGGAGAGTCTGCGTCAGTCTTTGATGAAACTAGAAAGACCACGAACTTCAAAGGGGAAAGCA
AGGCCAAAGACAGGGAGAAGAAAGCGTTCTGCAAAGCCTGAAACTGTAATTGACTCTTTACTGCAGTAAATGTTACAGACTTAAAGTCAC
AATAAAAATTAGTGATATTCTCATGCCTGGACAAAATCTTTAATTAAAACAGTGAAGACTTCTCTATAATTTCAATTAATGGAAGTTGTA
GATCAATGAATAAGACTGGAAATAATAATTTGCTTAAGAACTTTTAGTCTACATATATTAATATAACATTAAAATTGTACAACTGGTGAT
TGTTCAAATTGTTGTACTACTACTCTTCAATTGTACTGTGCAGGGAAGTTGGGGGAACAGTTCATACTATAGAGAGTTACAGTTTAGATG
TATGTGTAAATCGATTAGCTATTTATCCAACTACTGTATATTTAGGTAACTAGAATTTCAAAATAGAAAAAAAAAAACTGTGTCCTGTTT
TGCACATAGAAAGAAGCAGATCAGATTGTCCTATGTTGCGCTGTTATATATGAATGTTTGGACTGTACATCTAAAGAATGATTCCGTCCT
AACACCCACCCAGCCTGTTTGAACACATTCTAATTGTCTAACATTTGTTGCATTTTAAGTATGAGATGACTGATCTTATGAATGTTTTGT
AGAAAATGTTATAATTTAACAGTCTTTGCGAGTATACATGTTTTTTAACAAGTTGAAAATGTTTCATCTGAACCTTTCCTAACTTTTTTT
TGCCAGTTTGAGTTCAAATCAAATACACCTTAAGTTCTGGTTTCATGTTCTCTTGAAACATTGAATTGTACGTAAATATAAAGGATCTAC
TGTTGCGGAAAATTAAGAAGCTAAATACTGCTCAGTTATCTTTGCCTGCGTGTATATTTTTAAAAATTCACTCTGAAGTGTTATGGATGC
TTTTCTTTGAGTTCTAAATTTTGGTGGGAATTACTTTATGTCTCCCAGTTAAGAAGAGTCTCCATTTTCCAGAAAATACAGCTAAGCAAG

>In-frame_ENST00000285681_ENST00000378735_TCGA-L5-A8NE_USP25_chr21_17138460_+_KIF3A_chr5_132039311_length(amino acids)=439AA_start in transcript=189_stop in transcript=1508
MPAVPAHAGRHRLRAWLAGALSSQAVRAAPWSSAERGSQGRRRREEPGATAAAAAAAAGAMTVEQNVLQQSAAQKHQQTFLNQLREITGI
NDTQILQQALKDSNGNLELAVAFLTAKNAKTPQQEETTYYQTALPGNDRYISVGSQADTRKKKVSPDKMIEMQAKIDEERKALETKLDME
EEERNKARAELEKREKDLLKAQQEHQSLLEKLSALEKKVIVGGVDLLAKAEEQEKLLEESNMELEERRKRAEQLRRELEEKEQERLDIEE
KYTSLQEEAQGKTKKLKKVWTMLMAAKSEMADLQQEHQREIEGLLENIRQLSRELRLQMLIIDNFIPRDYQEMIENYVHWNEDIGEWQLK

--------------------------------------------------------------
>In-frame_ENST00000285681_ENST00000403231_TCGA-L5-A8NE_USP25_chr21_17138460_+_KIF3A_chr5_132039311_length(transcript)=1509nt_BP=637nt
AAGCAGCCCGCGGACCGGCAGCAAAGGAACGTGCGAACGCGTGACGCCGCCCGACTGGCTCGCGCTCTCCCGTGCCCCGGCGTCCTCCGC
CCGCTCATGGCCCGGGCCGCCGCGGACGAGCGGCGCTGAGGCGGGCCGCGTGGAGACGTGAGGCGGCCGCCGTGGCCCTCACAGTCGGCG
TTTCGCCGCCTGCCCGCGGTGCCCGCGCACGCCGGCCGCCATCGCCTTCGCGCCTGGCTGGCGGGGGCGCTGTCCTCCCAGGCCGTCCGC
GCCGCTCCCTGGAGCTCGGCGGAGCGCGGCAGCCAGGGCCGGCGGAGGCGCGAGGAGCCGGGCGCCACCGCCGCCGCCGCCGCCGCCGCC
GCGGGGGCCATGACCGTGGAGCAGAACGTGCTGCAGCAGAGCGCGGCGCAGAAGCACCAGCAGACGTTTTTGAATCAACTGAGAGAAATT
ACGGGGATTAATGACACCCAGATACTACAGCAAGCCTTGAAGGATAGTAATGGAAACTTGGAATTAGCAGTGGCTTTCCTTACTGCGAAG
AATGCTAAGACCCCTCAGCAGGAGGAGACAACTTACTACCAAACAGCACTTCCTGGCAATGATAGATACATCAGTGTGGGAAGCCAAGCA
GATACAAGAAAAAAGAAAGTCTCCCCAGACAAGATGATTGAAATGCAAGCAAAAATTGATGAGGAGAGAAAAGCACTTGAAACAAAGCTC
GACATGGAAGAAGAAGAAAGAAACAAGGCTAGAGCTGAATTAGAGAAACGGGAAAAAGATCTTCTTAAAGCCCAACAAGAGCATCAGTCT
TTGCTGGAAAAATTATCTGCCCTGGAAAAGAAGGTAATTGTTGGTGGGGTTGACTTGTTGGCCAAAGCTGAGGAACAAGAGAAACTTCTT
GAAGAATCTAACATGGAACTGGAAGAAAGGAGGAAAAGAGCAGAGCAACTTCGCAGAGAACTTGAGGAAAAAGAGCAAGAACGCTTGGAT
ATTGAAGAAAAATATACCAGTTTGCAAGAGGAAGCACAGGGAAAGACCAAGAAGTTAAAGAAAGTTTGGACTATGCTGATGGCTGCAAAG
TCAGAGATGGCTGATCTCCAACAAGAACATCAGAGGGAAATTGAAGGCCTACTGGAGAACATTCGGCAACTTAGCCGGGAGCTTCGACTT
CAGATGCTTATTATTGATAACTTTATACCTCGGGATTATCAGGAAATGATTGAAAACTATGTCCATTGGAATGAAGACATAGGAGAATGG
CAGCTAAAATGTGTTGCTTATACAGGAAATAACATGAGGAAGCAAACCCCAGTACCTGATAAAAAGGAGAAAGATCCCTTTGAGGTGGAC
CTTTCTCACGTGTATCTTGCCTATACTGAGGAGAGTCTGCGTCAGTCTTTGATGAAACTAGAAAGACCACGAACTTCAAAGGGGAAAGCA

>In-frame_ENST00000285681_ENST00000403231_TCGA-L5-A8NE_USP25_chr21_17138460_+_KIF3A_chr5_132039311_length(amino acids)=439AA_start in transcript=189_stop in transcript=1508
MPAVPAHAGRHRLRAWLAGALSSQAVRAAPWSSAERGSQGRRRREEPGATAAAAAAAAGAMTVEQNVLQQSAAQKHQQTFLNQLREITGI
NDTQILQQALKDSNGNLELAVAFLTAKNAKTPQQEETTYYQTALPGNDRYISVGSQADTRKKKVSPDKMIEMQAKIDEERKALETKLDME
EEERNKARAELEKREKDLLKAQQEHQSLLEKLSALEKKVIVGGVDLLAKAEEQEKLLEESNMELEERRKRAEQLRRELEEKEQERLDIEE
KYTSLQEEAQGKTKKLKKVWTMLMAAKSEMADLQQEHQREIEGLLENIRQLSRELRLQMLIIDNFIPRDYQEMIENYVHWNEDIGEWQLK

--------------------------------------------------------------
>In-frame_ENST00000400183_ENST00000378746_TCGA-L5-A8NE_USP25_chr21_17138460_+_KIF3A_chr5_132039311_length(transcript)=5363nt_BP=485nt
GCGGCCGCCGTGGCCCTCACAGTCGGCGTTTCGCCGCCTGCCCGCGGTGCCCGCGCACGCCGGCCGCCATCGCCTTCGCGCCTGGCTGGC
GGGGGCGCTGTCCTCCCAGGCCGTCCGCGCCGCTCCCTGGAGCTCGGCGGAGCGCGGCAGCCAGGGCCGGCGGAGGCGCGAGGAGCCGGG
CGCCACCGCCGCCGCCGCCGCCGCCGCCGCGGGGGCCATGACCGTGGAGCAGAACGTGCTGCAGCAGAGCGCGGCGCAGAAGCACCAGCA
GACGTTTTTGAATCAACTGAGAGAAATTACGGGGATTAATGACACCCAGATACTACAGCAAGCCTTGAAGGATAGTAATGGAAACTTGGA
ATTAGCAGTGGCTTTCCTTACTGCGAAGAATGCTAAGACCCCTCAGCAGGAGGAGACAACTTACTACCAAACAGCACTTCCTGGCAATGA
TAGATACATCAGTGTGGGAAGCCAAGCAGATACAAGAAAAAAGAAAGTCTCCCCAGACAAGATGATTGAAATGCAAGCAAAAATTGATGA
GGAGAGAAAAGCACTTGAAACAAAGCTCGACATGGAAGAAGAAGAAAGAAACAAGGCTAGAGCTGAATTAGAGAAACGGGAAAAAGATCT
TCTTAAAGCCCAACAAGAGCATCAGTCTTTGCTGGAAAAATTATCTGCCCTGGAAAAGAAGGTAATTGTTGGTGGGGTTGACTTGTTGGC
CAAAGCTGAGGAACAAGAGAAACTTCTTGAAGAATCTAACATGGAACTGGAAGAAAGGAGGAAAAGAGCAGAGCAACTTCGCAGAGAACT
TGAGGAAAAAGAGCAAGAACGCTTGGATATTGAAGAAAAATATACCAGTTTGCAAGAGGAAGCACAGGGAAAGACCAAGAAGTTAAAGAA
AGTTTGGACTATGCTGATGGCTGCAAAGTCAGAGATGGCTGATCTCCAACAAGAACATCAGAGGGAAATTGAAGGCCTACTGGAGAACAT
TCGGCAACTTAGCCGGGAGCTTCGACTTCAGATGCTTATTATTGATAACTTTATACCTCGGGATTATCAGGAAATGATTGAAAACTATGT
CCATTGGAATGAAGACATAGGAGAATGGCAGCTAAAATGTGTTGCTTATACAGGAAATAACATGAGGAAGCAAACCCCAGTACCTGATAA
AAAGGAGAAAGATCCCTTTGAGGTGGACCTTTCTCACGTGTATCTTGCCTATACTGAGGAGAGTCTGCGTCAGTCTTTGATGAAACTAGA
AAGACCACGAACTTCAAAGGGGAAAGCAAGGCCAAAGACAGGGAGAAGAAAGCGTTCTGCAAAGCCTGAAACTGTAATTGACTCTTTACT
GCAGTAAATGTTACAGACTTAAAGTCACAATAAAAATTAGTGATATTCTCATGCCTGGACAAAATCTTTAATTAAAACAGTGAAGACTTC
TCTATAATTTCAATTAATGGAAGTTGTAGATCAATGAATAAGACTGGAAATAATAATTTGCTTAAGAACTTTTAGTCTACATATATTAAT
ATAACATTAAAATTGTACAACTGGTGATTGTTCAAATTGTTGTACTACTACTCTTCAATTGTACTGTGCAGGGAAGTTGGGGGAACAGTT
CATACTATAGAGAGTTACAGTTTAGATGTATGTGTAAATCGATTAGCTATTTATCCAACTACTGTATATTTAGGTAACTAGAATTTCAAA
ATAGAAAAAAAAAAACTGTGTCCTGTTTTGCACATAGAAAGAAGCAGATCAGATTGTCCTATGTTGCGCTGTTATATATGAATGTTTGGA
CTGTACATCTAAAGAATGATTCCGTCCTAACACCCACCCAGCCTGTTTGAACACATTCTAATTGTCTAACATTTGTTGCATTTTAAGTAT
GAGATGACTGATCTTATGAATGTTTTGTAGAAAATGTTATAATTTAACAGTCTTTGCGAGTATACATGTTTTTTAACAAGTTGAAAATGT
TTCATCTGAACCTTTCCTAACTTTTTTTTGCCAGTTTGAGTTCAAATCAAATACACCTTAAGTTCTGGTTTCATGTTCTCTTGAAACATT
GAATTGTACGTAAATATAAAGGATCTACTGTTGCGGAAAATTAAGAAGCTAAATACTGCTCAGTTATCTTTGCCTGCGTGTATATTTTTA
AAAATTCACTCTGAAGTGTTATGGATGCTTTTCTTTGAGTTCTAAATTTTGGTGGGAATTACTTTATGTCTCCCAGTTAAGAAGAGTCTC
CATTTTCCAGAAAATACAGCTAAGCAAGAAAAATATCTTTTAAATACTTTGCCTTTAAAACACTTTTTTATTTTGTTTTTCTATTTTAAA
AATAAAATATTATTTTCTGAATTTCTTCTATTGGGTAATCCAGTTAGACCATATTCCTCTACAAATAAAAACTATGTTGATTTCATAGTA
AAGTAAGTTGTAATAGAGATAATTATTTTTTTCTGTTGTTAGCTGCTTATGAAACTTTTATTGTATTTTTTTAAAGTTATGCTACTCCTG
GCCGGGCATGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGCCGAGGCACGTGGATCACTTGATGTCAGGAGTTCGAGACCAGCCT
GGCTAACATGGTGAAACCCTGTCTCCAGTACAAATACAATAATTAGCTGGGTGTGGTGGCGCACACCTGTAATCCCAGCTACTTGGGAGG
CTGAGGCAGGAGAATCACTTGAACCTGGGAGGCGGAGGTTGCAGTGAGCTGAGATGGCATCACTGCACTCCAGCCTGGGTGACAGGGTGA
CACTCCACCTCAAAAAATAAAAATAAAATAAAAATAAAGTTATGCTACTCCTAAACCACTTAATATATGTTTTGTGGTTTTCAAAATTAT
GTAATCAGCCCTGAAACACATTGGTTGAAAATATTTTGAGACTTTATAAGTTAGTATTTATTAAATTAATATTATTTTAATAAGTTTTGT
TAAATCCTAGTTTAAATGACAAAGCTGTGTATAGAGTAGGGGTGAGTGAAGGTGGAGTACTCTTGAGGTGGCGATAAAAATGTACCCATA
AATTTAAAGTCCATTCTGTAATTGGAATACTGAATGTCCTGTGTGTGGTGAACATTTTCTAGGTGATTAGAATTGCATTTCACTGGCTGC
ATTTGTTTGTGGTATGTAAGATAGCTAGAAATTTATTACTTAACTCTGTGGGGCATTGTTTAGATACTGTGAAGAGTTGAGGTAAATATG
TGTCTTGTAAAAGCTTTGTGTGATCAGAATATTTTGGTAATTATAAAATGTTTTTGATGATACACATTTAACTCTGTTAGTAATTACTCT
AAAGAAATCTTAATTTTACCAGTTTAAAAAAAACGCTTCTTTTTAAAAGTTCATGTTTCTAACCTTTAACAATTCAGTTAATAGGAAAGG
AGTAACTTTTAAATCAAGCTTTAAAACATGGAAATTTAAGCCAGTATGTTAGATATTTTTTCTATCCCACCCTACCATGATAATAAAAAT
AAATCTATAAAAAGTCAAAATAAAAAGCCGATCTATAAAAAGTCAATTGAGAAGTTCTAAAAGCAGGCTACTATAGCAGGGGTGTTTGTT
TTTTGTTTGGAGACGGAGTCTGGCTCTATTACCTAGGCTGGAGTGCAGTGGGACAATCTCGGCTCACTGTAACCTCTGTCTCTTCAGCCT
CCTGAGTTGCTGGAACTACAGCTGTATGCCATCATGCTCGGCTAATTTTTGTATTTTTTTGTAGAGATGGGGTTTTGCCATGTTGCCCGG
GCCTGTCTTGAACTCCTAGAGCTTAAGTGATCCACCCGCCTCAGGCTCCCGAAGTGCTGGGATTACAGGCATGATTATAGGCCTGAGCCA
CCACACCCCGCCTACTATAGCAGTTTTTTAAAAAAAGCATTATGTTAAAACTGATTATACCAGATGGACATTAAATATCCATCTCAGAAA
TACCAAAATTTGATACATAAGCCTGGCCTGAATATCTTTTTTTTTTTTTTGAGACGGAGTCTTACTCTGTCGCCCAGGCTGGAGTGCAGT
GGCGCGATCTCGGCTCACTGCAACCTCTGCCTCCTGGGTTCAAGGGATTCTCCTGCCTCAGCCTCTTGAGTAGCTGGGATTACAGGTTTG
CGCCACCACACCCAGCTAATTTTTGTATTTTTTTTTTAGTAGAGACCGGGTTTCACCATGTTGGCCAGGCTGGTCTCACCTGACTTTGTG
ATCCGCCCGCCTCAGCCTCCCAAAGTGCTGGGATTACAGGTGTGAGCCACCACGCCTGGCCTCAAGCCTGGTCTGAATATCTATATGCAA
ATATGGGACACTGACTTCAGAGCTATTCATTTAGCTCCAGTCATAGCAATTCTGTAGTTTCCTCACATTTACTGTGTCTTTGTCCACAAA
TGAAATATGTTGTAAGGTCAGTGTTAATTTCGTGAGACGATATAGATGAACACACTTGCTCATTTTTTGAAATAAATATGCAGATTTAAA
ATGAGTGGCACATATATATAAATAGGTTATTGAATGGGTATTCTCTTTTCCCTTTAATTTAAAAAGTGATATTTATTTATTAAAAGGAGC
ATTGGTAGAATTATTGGTTTATAGTATCTTCTTTAATAATCCTTACAATTACATATACTAATAGGATGTTAAAATTATCATCGTGTTTCC
TATTTGTGTTTAGATTCTTATAAAGCATGAGATGAGTAATCAAGGGAAGGGTCGCATCTCCATGTTTGCCCCATGAATAGAATCCATTTG
ACAAGCCATTTCATTTCCTTTCTGGAGAAAGGGAGTTGTTTTTTTTTTCTTTTTACAGCAAAAATTTAAGGTTTTATTGGGGGAGGGGGT
GGTGCTACTTCCTCTGTAACATGTTTTTAAAACTATGATTATGGTTTTTGGTTGTTTTTTGTTTTGTTTGTTTCCTTTTAGTCTGTTTTT
AATATCCTTCCCCTTAACTCTTAAGGAAAGTCTTCTTTCCTACAGGGCTGTGAATTAAGGAATGGGTAGCGTTTACATTTAAGTACACCA
ATGTCTGCCCAATTTCCTAATGTATACATTTGTTTTATTTTAAATTATTTTTTGTTCTTAAAAATACTAGAAGAAAACATAGATGTTTCT
AGTGCCTTTGAACTTATACTGTAGTCTGACTAATTTAATGAATAACTTCAGTAGCATTACTATAATACTGTATGCTGGCCAAAATTACAA
ATTACAGTTTGTTGCATCAGTTTCAAAAGCCATGCTTTCATTGTATTTTATGTATAAATTGTATTTGTTCAAGTTGTATATATTGGTATT

>In-frame_ENST00000400183_ENST00000378746_TCGA-L5-A8NE_USP25_chr21_17138460_+_KIF3A_chr5_132039311_length(amino acids)=439AA_start in transcript=37_stop in transcript=1356
MPAVPAHAGRHRLRAWLAGALSSQAVRAAPWSSAERGSQGRRRREEPGATAAAAAAAAGAMTVEQNVLQQSAAQKHQQTFLNQLREITGI
NDTQILQQALKDSNGNLELAVAFLTAKNAKTPQQEETTYYQTALPGNDRYISVGSQADTRKKKVSPDKMIEMQAKIDEERKALETKLDME
EEERNKARAELEKREKDLLKAQQEHQSLLEKLSALEKKVIVGGVDLLAKAEEQEKLLEESNMELEERRKRAEQLRRELEEKEQERLDIEE
KYTSLQEEAQGKTKKLKKVWTMLMAAKSEMADLQQEHQREIEGLLENIRQLSRELRLQMLIIDNFIPRDYQEMIENYVHWNEDIGEWQLK

--------------------------------------------------------------
>In-frame_ENST00000400183_ENST00000378735_TCGA-L5-A8NE_USP25_chr21_17138460_+_KIF3A_chr5_132039311_length(transcript)=2361nt_BP=485nt
GCGGCCGCCGTGGCCCTCACAGTCGGCGTTTCGCCGCCTGCCCGCGGTGCCCGCGCACGCCGGCCGCCATCGCCTTCGCGCCTGGCTGGC
GGGGGCGCTGTCCTCCCAGGCCGTCCGCGCCGCTCCCTGGAGCTCGGCGGAGCGCGGCAGCCAGGGCCGGCGGAGGCGCGAGGAGCCGGG
CGCCACCGCCGCCGCCGCCGCCGCCGCCGCGGGGGCCATGACCGTGGAGCAGAACGTGCTGCAGCAGAGCGCGGCGCAGAAGCACCAGCA
GACGTTTTTGAATCAACTGAGAGAAATTACGGGGATTAATGACACCCAGATACTACAGCAAGCCTTGAAGGATAGTAATGGAAACTTGGA
ATTAGCAGTGGCTTTCCTTACTGCGAAGAATGCTAAGACCCCTCAGCAGGAGGAGACAACTTACTACCAAACAGCACTTCCTGGCAATGA
TAGATACATCAGTGTGGGAAGCCAAGCAGATACAAGAAAAAAGAAAGTCTCCCCAGACAAGATGATTGAAATGCAAGCAAAAATTGATGA
GGAGAGAAAAGCACTTGAAACAAAGCTCGACATGGAAGAAGAAGAAAGAAACAAGGCTAGAGCTGAATTAGAGAAACGGGAAAAAGATCT
TCTTAAAGCCCAACAAGAGCATCAGTCTTTGCTGGAAAAATTATCTGCCCTGGAAAAGAAGGTAATTGTTGGTGGGGTTGACTTGTTGGC
CAAAGCTGAGGAACAAGAGAAACTTCTTGAAGAATCTAACATGGAACTGGAAGAAAGGAGGAAAAGAGCAGAGCAACTTCGCAGAGAACT
TGAGGAAAAAGAGCAAGAACGCTTGGATATTGAAGAAAAATATACCAGTTTGCAAGAGGAAGCACAGGGAAAGACCAAGAAGTTAAAGAA
AGTTTGGACTATGCTGATGGCTGCAAAGTCAGAGATGGCTGATCTCCAACAAGAACATCAGAGGGAAATTGAAGGCCTACTGGAGAACAT
TCGGCAACTTAGCCGGGAGCTTCGACTTCAGATGCTTATTATTGATAACTTTATACCTCGGGATTATCAGGAAATGATTGAAAACTATGT
CCATTGGAATGAAGACATAGGAGAATGGCAGCTAAAATGTGTTGCTTATACAGGAAATAACATGAGGAAGCAAACCCCAGTACCTGATAA
AAAGGAGAAAGATCCCTTTGAGGTGGACCTTTCTCACGTGTATCTTGCCTATACTGAGGAGAGTCTGCGTCAGTCTTTGATGAAACTAGA
AAGACCACGAACTTCAAAGGGGAAAGCAAGGCCAAAGACAGGGAGAAGAAAGCGTTCTGCAAAGCCTGAAACTGTAATTGACTCTTTACT
GCAGTAAATGTTACAGACTTAAAGTCACAATAAAAATTAGTGATATTCTCATGCCTGGACAAAATCTTTAATTAAAACAGTGAAGACTTC
TCTATAATTTCAATTAATGGAAGTTGTAGATCAATGAATAAGACTGGAAATAATAATTTGCTTAAGAACTTTTAGTCTACATATATTAAT
ATAACATTAAAATTGTACAACTGGTGATTGTTCAAATTGTTGTACTACTACTCTTCAATTGTACTGTGCAGGGAAGTTGGGGGAACAGTT
CATACTATAGAGAGTTACAGTTTAGATGTATGTGTAAATCGATTAGCTATTTATCCAACTACTGTATATTTAGGTAACTAGAATTTCAAA
ATAGAAAAAAAAAAACTGTGTCCTGTTTTGCACATAGAAAGAAGCAGATCAGATTGTCCTATGTTGCGCTGTTATATATGAATGTTTGGA
CTGTACATCTAAAGAATGATTCCGTCCTAACACCCACCCAGCCTGTTTGAACACATTCTAATTGTCTAACATTTGTTGCATTTTAAGTAT
GAGATGACTGATCTTATGAATGTTTTGTAGAAAATGTTATAATTTAACAGTCTTTGCGAGTATACATGTTTTTTAACAAGTTGAAAATGT
TTCATCTGAACCTTTCCTAACTTTTTTTTGCCAGTTTGAGTTCAAATCAAATACACCTTAAGTTCTGGTTTCATGTTCTCTTGAAACATT
GAATTGTACGTAAATATAAAGGATCTACTGTTGCGGAAAATTAAGAAGCTAAATACTGCTCAGTTATCTTTGCCTGCGTGTATATTTTTA
AAAATTCACTCTGAAGTGTTATGGATGCTTTTCTTTGAGTTCTAAATTTTGGTGGGAATTACTTTATGTCTCCCAGTTAAGAAGAGTCTC
CATTTTCCAGAAAATACAGCTAAGCAAGAAAAATATCTTTTAAATACTTTGCCTTTAAAACACTTTTTTATTTTGTTTTTCTATTTTAAA

>In-frame_ENST00000400183_ENST00000378735_TCGA-L5-A8NE_USP25_chr21_17138460_+_KIF3A_chr5_132039311_length(amino acids)=439AA_start in transcript=37_stop in transcript=1356
MPAVPAHAGRHRLRAWLAGALSSQAVRAAPWSSAERGSQGRRRREEPGATAAAAAAAAGAMTVEQNVLQQSAAQKHQQTFLNQLREITGI
NDTQILQQALKDSNGNLELAVAFLTAKNAKTPQQEETTYYQTALPGNDRYISVGSQADTRKKKVSPDKMIEMQAKIDEERKALETKLDME
EEERNKARAELEKREKDLLKAQQEHQSLLEKLSALEKKVIVGGVDLLAKAEEQEKLLEESNMELEERRKRAEQLRRELEEKEQERLDIEE
KYTSLQEEAQGKTKKLKKVWTMLMAAKSEMADLQQEHQREIEGLLENIRQLSRELRLQMLIIDNFIPRDYQEMIENYVHWNEDIGEWQLK

--------------------------------------------------------------
>In-frame_ENST00000400183_ENST00000403231_TCGA-L5-A8NE_USP25_chr21_17138460_+_KIF3A_chr5_132039311_length(transcript)=1357nt_BP=485nt
GCGGCCGCCGTGGCCCTCACAGTCGGCGTTTCGCCGCCTGCCCGCGGTGCCCGCGCACGCCGGCCGCCATCGCCTTCGCGCCTGGCTGGC
GGGGGCGCTGTCCTCCCAGGCCGTCCGCGCCGCTCCCTGGAGCTCGGCGGAGCGCGGCAGCCAGGGCCGGCGGAGGCGCGAGGAGCCGGG
CGCCACCGCCGCCGCCGCCGCCGCCGCCGCGGGGGCCATGACCGTGGAGCAGAACGTGCTGCAGCAGAGCGCGGCGCAGAAGCACCAGCA
GACGTTTTTGAATCAACTGAGAGAAATTACGGGGATTAATGACACCCAGATACTACAGCAAGCCTTGAAGGATAGTAATGGAAACTTGGA
ATTAGCAGTGGCTTTCCTTACTGCGAAGAATGCTAAGACCCCTCAGCAGGAGGAGACAACTTACTACCAAACAGCACTTCCTGGCAATGA
TAGATACATCAGTGTGGGAAGCCAAGCAGATACAAGAAAAAAGAAAGTCTCCCCAGACAAGATGATTGAAATGCAAGCAAAAATTGATGA
GGAGAGAAAAGCACTTGAAACAAAGCTCGACATGGAAGAAGAAGAAAGAAACAAGGCTAGAGCTGAATTAGAGAAACGGGAAAAAGATCT
TCTTAAAGCCCAACAAGAGCATCAGTCTTTGCTGGAAAAATTATCTGCCCTGGAAAAGAAGGTAATTGTTGGTGGGGTTGACTTGTTGGC
CAAAGCTGAGGAACAAGAGAAACTTCTTGAAGAATCTAACATGGAACTGGAAGAAAGGAGGAAAAGAGCAGAGCAACTTCGCAGAGAACT
TGAGGAAAAAGAGCAAGAACGCTTGGATATTGAAGAAAAATATACCAGTTTGCAAGAGGAAGCACAGGGAAAGACCAAGAAGTTAAAGAA
AGTTTGGACTATGCTGATGGCTGCAAAGTCAGAGATGGCTGATCTCCAACAAGAACATCAGAGGGAAATTGAAGGCCTACTGGAGAACAT
TCGGCAACTTAGCCGGGAGCTTCGACTTCAGATGCTTATTATTGATAACTTTATACCTCGGGATTATCAGGAAATGATTGAAAACTATGT
CCATTGGAATGAAGACATAGGAGAATGGCAGCTAAAATGTGTTGCTTATACAGGAAATAACATGAGGAAGCAAACCCCAGTACCTGATAA
AAAGGAGAAAGATCCCTTTGAGGTGGACCTTTCTCACGTGTATCTTGCCTATACTGAGGAGAGTCTGCGTCAGTCTTTGATGAAACTAGA
AAGACCACGAACTTCAAAGGGGAAAGCAAGGCCAAAGACAGGGAGAAGAAAGCGTTCTGCAAAGCCTGAAACTGTAATTGACTCTTTACT

>In-frame_ENST00000400183_ENST00000403231_TCGA-L5-A8NE_USP25_chr21_17138460_+_KIF3A_chr5_132039311_length(amino acids)=440AA_start in transcript=37_stop in transcript=1356
MPAVPAHAGRHRLRAWLAGALSSQAVRAAPWSSAERGSQGRRRREEPGATAAAAAAAAGAMTVEQNVLQQSAAQKHQQTFLNQLREITGI
NDTQILQQALKDSNGNLELAVAFLTAKNAKTPQQEETTYYQTALPGNDRYISVGSQADTRKKKVSPDKMIEMQAKIDEERKALETKLDME
EEERNKARAELEKREKDLLKAQQEHQSLLEKLSALEKKVIVGGVDLLAKAEEQEKLLEESNMELEERRKRAEQLRRELEEKEQERLDIEE
KYTSLQEEAQGKTKKLKKVWTMLMAAKSEMADLQQEHQREIEGLLENIRQLSRELRLQMLIIDNFIPRDYQEMIENYVHWNEDIGEWQLK

--------------------------------------------------------------

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Fusion Gene PPI Analysis for USP25-KIF3A


check button Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in

ChiPPI page.


check button Protein-protein interactors with each fusion partner protein in wild-type (BIOGRID-3.4.160)
HgeneHgene's interactorsTgeneTgene's interactors


check button - Retained PPIs in in-frame fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenStill interaction with


check button - Lost PPIs in in-frame fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenInteraction lost with


check button - Retained PPIs, but lost function due to frame-shift fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenInteraction lost with


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Related Drugs for USP25-KIF3A


check button Drugs targeting genes involved in this fusion gene.
(DrugBank Version 5.1.8 2021-05-08)
PartnerGeneUniProtAccDrugBank IDDrug nameDrug activityDrug typeDrug status

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Related Diseases for USP25-KIF3A


check button Diseases associated with fusion partners.
(DisGeNet 4.0)
PartnerGeneDisease IDDisease name# pubmedsSource
TgeneKIF3AC0004096Asthma1CTD_human
TgeneKIF3AC0005941Bone Diseases, Developmental1CTD_human
TgeneKIF3AC0152427Polydactyly1CTD_human
TgeneKIF3AC0238198Gastrointestinal Stromal Tumors1CTD_human
TgeneKIF3AC0376634Craniofacial Abnormalities1CTD_human
TgeneKIF3AC3179349Gastrointestinal Stromal Sarcoma1CTD_human