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 | Fusion Gene Summary |
| Fusion Gene ORF analysis |
| Fusion Genomic Features |
| Fusion Protein Features |
| Fusion Gene Sequence |
| Fusion Gene PPI analysis |
| Related Drugs |
| Related Diseases |
Fusion gene:USP32-EXOSC5 (FusionGDB2 ID:96698) |
Fusion Gene Summary for USP32-EXOSC5 |
 Fusion gene summary |
| Fusion gene information | Fusion gene name: USP32-EXOSC5 | Fusion gene ID: 96698 | Hgene | Tgene | Gene symbol | USP32 | EXOSC5 | Gene ID | 84669 | 56915 |
| Gene name | ubiquitin specific peptidase 32 | exosome component 5 | |
| Synonyms | NY-REN-60|USP10 | RRP41B|RRP46|Rrp46p|hRrp46p|p12B | |
| Cytomap | 17q23.1-q23.2 | 19q13.2 | |
| Type of gene | protein-coding | protein-coding | |
| Description | ubiquitin carboxyl-terminal hydrolase 32deubiquitinating enzyme 32renal carcinoma antigen NY-REN-60ubiquitin specific protease 32ubiquitin thioesterase 32ubiquitin thiolesterase 32ubiquitin-specific-processing protease 32 | exosome complex component RRP46chronic myelogenous leukemia tumor antigen 28exosome complex exonuclease RRP46exosome component Rrp46ribosomal RNA-processing protein 46 | |
| Modification date | 20200313 | 20200313 | |
| UniProtAcc | . | Q9NQT4 | |
| Ensembl transtripts involved in fusion gene | ENST00000300896, ENST00000592339, ENST00000393003, ENST00000586881, | ENST00000221233, ENST00000596905, | |
| Fusion gene scores | * DoF score | 36 X 25 X 11=9900 | 3 X 3 X 3=27 |
| # samples | 42 | 3 | |
| ** MAII score | log2(42/9900*10)=-4.55896729218821 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0 | log2(3/27*10)=0.15200309344505 effective Gene in Pan-Cancer Fusion Genes (eGinPCFGs). DoF>8 and MAII>0 | |
| Context | PubMed: USP32 [Title/Abstract] AND EXOSC5 [Title/Abstract] AND fusion [Title/Abstract] | ||
| Most frequent breakpoint | USP32(58329742)-EXOSC5(41898885), # samples:1 | ||
| Anticipated loss of major functional domain due to fusion event. | USP32-EXOSC5 seems lost the major protein functional domain in Hgene partner, which is a essential gene due to the frame-shifted ORF. USP32-EXOSC5 seems lost the major protein functional domain in Tgene partner, which is a cell metabolism gene due to the frame-shifted ORF. USP32-EXOSC5 seems lost the major protein functional domain in Tgene partner, which is a essential gene due to the frame-shifted ORF. USP32-EXOSC5 seems lost the major protein functional domain in Tgene partner, which is a epigenetic factor due to the frame-shifted ORF. | ||
| * DoF score (Degree of Frequency) = # partners X # break points X # cancer types ** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10) |
| Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez |
| Partner | Gene | GO ID | GO term | PubMed ID |
| Tgene | EXOSC5 | GO:0045006 | DNA deamination | 21255825 |
| Fusion gene breakpoints across USP32 (5'-gene) * Click on the image to open the UCSC genome browser with custom track showing this image in a new window. |
| Fusion gene breakpoints across EXOSC5 (3'-gene) * Click on the image to open the UCSC genome browser with custom track showing this image in a new window. |
| Fusion gene information from two resources (ChiTars 5.0 and ChimerDB 4.0) * All genome coordinats were lifted-over on hg19. * Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser. |
| Source | Disease | Sample | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand |
| ChimerDB4 | KIRP | TCGA-IA-A40Y-01A | USP32 | chr17 | 58329742 | - | EXOSC5 | chr19 | 41898885 | - |
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Fusion Gene ORF analysis for USP32-EXOSC5 |
| Open reading frame (ORF) analsis of fusion genes based on Ensembl gene isoform structure. * Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser. |
| ORF | Henst | Tenst | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand |
| Frame-shift | ENST00000300896 | ENST00000221233 | USP32 | chr17 | 58329742 | - | EXOSC5 | chr19 | 41898885 | - |
| 5CDS-intron | ENST00000300896 | ENST00000596905 | USP32 | chr17 | 58329742 | - | EXOSC5 | chr19 | 41898885 | - |
| In-frame | ENST00000592339 | ENST00000221233 | USP32 | chr17 | 58329742 | - | EXOSC5 | chr19 | 41898885 | - |
| 5CDS-intron | ENST00000592339 | ENST00000596905 | USP32 | chr17 | 58329742 | - | EXOSC5 | chr19 | 41898885 | - |
| Frame-shift | ENST00000393003 | ENST00000221233 | USP32 | chr17 | 58329742 | - | EXOSC5 | chr19 | 41898885 | - |
| 5CDS-intron | ENST00000393003 | ENST00000596905 | USP32 | chr17 | 58329742 | - | EXOSC5 | chr19 | 41898885 | - |
| intron-3CDS | ENST00000586881 | ENST00000221233 | USP32 | chr17 | 58329742 | - | EXOSC5 | chr19 | 41898885 | - |
| intron-intron | ENST00000586881 | ENST00000596905 | USP32 | chr17 | 58329742 | - | EXOSC5 | chr19 | 41898885 | - |
| ORFfinder result based on the fusion transcript sequence of in-frame fusion genes. |
| Henst | Tenst | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand | Seq length (transcript) | BP loci (transcript) | Predicted start (transcript) | Predicted stop (transcript) | Seq length (amino acids) |
| ENST00000592339 | USP32 | chr17 | 58329742 | - | ENST00000221233 | EXOSC5 | chr19 | 41898885 | - | 1035 | 216 | 230 | 775 | 181 |
| DeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated. |
| Henst | Tenst | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand | No-coding score | Coding score |
| ENST00000592339 | ENST00000221233 | USP32 | chr17 | 58329742 | - | EXOSC5 | chr19 | 41898885 | - | 0.088185154 | 0.9118148 |
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Fusion Genomic Features for USP32-EXOSC5 |
| FusionAI prediction of the potential fusion gene breakpoint based on the pre-mature RNA sequence context (+/- 5kb of individual partner genes, total 20kb length sequence). FusionAI is a fusion gene breakpoint classifier based on convolutional neural network by comparing the fusion positive and negative sequence context of ~ 20K fusion gene data. From here, we can have the relative potentency of the 20K genomic sequence how individual sequnce will be likely used as the gene fusion breakpoints. |
| Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand | 1-p | p (fusion gene breakpoint) |
| Distribution of 44 human genomic features loci across 20kb length fusion breakpoint regions. We integrated a total of 44 different types of human genomic feature loci information across five big categories including virus integration sites, repeats, structural variants, chromatin states, and gene expression regulation. More details are in help page. |
| Distribution of 44 human genomic features loci across 20kb length fusion breakpoint regions that are ovelapped with the top 1% feature importance score regions. More details are in help page. |
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Fusion Protein Features for USP32-EXOSC5 |
| Go to FGviewer for the breakpoints of chr17:58329742-chr19:41898885 - FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels. |
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| Main function of each fusion partner protein. (from UniProt) |
| Hgene | Tgene |
| . | EXOSC5 |
| FUNCTION: Might normally function as a transcriptional repressor. EWS-fusion-proteins (EFPS) may play a role in the tumorigenic process. They may disturb gene expression by mimicking, or interfering with the normal function of CTD-POLII within the transcription initiation complex. They may also contribute to an aberrant activation of the fusion protein target genes. | FUNCTION: Non-catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity and participates in a multitude of cellular RNA processing and degradation events. In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snRNA and snoRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts, such as antisense RNA species and promoter-upstream transcripts (PROMPTs), and of mRNAs with processing defects, thereby limiting or excluding their export to the cytoplasm. The RNA exosome may be involved in Ig class switch recombination (CSR) and/or Ig variable region somatic hypermutation (SHM) by targeting AICDA deamination activity to transcribed dsDNA substrates. In the cytoplasm, the RNA exosome complex is involved in general mRNA turnover and specifically degrades inherently unstable mRNAs containing AU-rich elements (AREs) within their 3' untranslated regions, and in RNA surveillance pathways, preventing translation of aberrant mRNAs. It seems to be involved in degradation of histone mRNA. The catalytic inactive RNA exosome core complex of 9 subunits (Exo-9) is proposed to play a pivotal role in the binding and presentation of RNA for ribonucleolysis, and to serve as a scaffold for the association with catalytic subunits and accessory proteins or complexes. {ECO:0000269|PubMed:11782436, ECO:0000269|PubMed:21255825}. |
| Retention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at * Minus value of BPloci means that the break pointn is located before the CDS. |
| - In-frame and retained protein feature among the 13 regional features. |
| Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Protein feature | Protein feature note |
| Hgene | USP32 | chr17:58329742 | chr19:41898885 | ENST00000300896 | - | 11 | 34 | 241_252 | 378.6666666666667 | 1605.0 | Calcium binding | 1 |
| Hgene | USP32 | chr17:58329742 | chr19:41898885 | ENST00000300896 | - | 11 | 34 | 277_288 | 378.6666666666667 | 1605.0 | Calcium binding | 2 |
| Hgene | USP32 | chr17:58329742 | chr19:41898885 | ENST00000300896 | - | 11 | 34 | 228_263 | 378.6666666666667 | 1605.0 | Domain | EF-hand 2 |
| Hgene | USP32 | chr17:58329742 | chr19:41898885 | ENST00000300896 | - | 11 | 34 | 264_299 | 378.6666666666667 | 1605.0 | Domain | EF-hand 3 |
| Hgene | USP32 | chr17:58329742 | chr19:41898885 | ENST00000300896 | - | 11 | 34 | 91_126 | 378.6666666666667 | 1605.0 | Domain | EF-hand 1 |
| - In-frame and not-retained protein feature among the 13 regional features. |
| Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Protein feature | Protein feature note |
| Hgene | USP32 | chr17:58329742 | chr19:41898885 | ENST00000300896 | - | 11 | 34 | 369_585 | 378.6666666666667 | 1605.0 | Domain | DUSP |
| Hgene | USP32 | chr17:58329742 | chr19:41898885 | ENST00000300896 | - | 11 | 34 | 734_1567 | 378.6666666666667 | 1605.0 | Domain | Note=USP |
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Fusion Gene Sequence for USP32-EXOSC5 |
| For in-frame fusion transcripts, we provide the fusion transcript sequences and fusion amino acid sequences. To have fusion amino acid sequence, we ran ORFfinder and chose the longest ORF among the all predicted ones. |
| >In-frame_ENST00000592339_ENST00000221233_TCGA-IA-A40Y-01A_USP32_chr17_58329742_-_EXOSC5_chr19_41898885_length(transcript)=1035nt_BP=216nt GTTCCTTTCCTCACAGCAACAGATACCACACTACAGTGGGACAATTTAGACACTCCTGTTAACCTAACGTATGGGTCATCTTACTCTGGA AGACTATCAGATCTGGAGTGTGAAAAATGTTCTTGCCAATGAGTTTTTGAACCTCCTTTTCCAGGTGTGTCACATAGTTCTGGGGTTAAG ACCAGCTACTCCGGAAGAAGAAGGACAAATTATTAGGTGACACCTCTGTCCTGGCGGGTGTGTACGGGCCGGCCGAGGTGAAGGTCAGCA AAGAGATTTTCAACAAGGCCACACTCGAAGTGATCCTGAGGCCGAAGATTGGGCTGCCTGGTGTTGCAGAGAAGAGCCGGGAGCGGCTGA TCAGGAACACGTGCGAGGCGGTGGTGCTGGGCACGTTGCACCCCCGCACCTCCATCACCGTGGTGCTGCAGGTTGTCAGCGATGCCGGCT CTCTCCTGGCCTGTTGTCTGAATGCCGCCTGCATGGCATTGGTGGATGCAGGTGTGCCCATGCGGGCTCTCTTCTGTGGGGTCGCCTGCG CCCTGGACTCTGATGGGACCCTCGTGCTGGATCCTACATCCAAGCAAGAAAAGGAGGCCCGGGCAGTCCTGACCTTTGCCCTGGACAGCG TGGAACGGAAGCTGCTGATGTCCAGCACCAAGGGGCTCTACTCAGACACTGAGCTCCAGCAGTGCCTGGCTGCGGCCCAGGCCGCTTCGC AACACGTCTTCCGTTTCTACCGGGAATCGCTGCAGAGGCGTTACTCCAAGAGCTGAGGCAAGCTGGGGCAAGGGGCCGCTCCCATTGCCT CCACCCACTCACCCCCTACAGCCTGAAGCAAACCAGCAGCCCAGCCTTGCCTCTCTGACCCATGGGCTCCTTGAGCCTGCAGCTCTGTAA CCACAGGGCTCCTGTGGGGAGGCCTTGGCCTGTGACAGCCCCCAGGCCTGGGGGCACAGATCCCCCCAGCAAGGATAACATTCAAAGGAG >In-frame_ENST00000592339_ENST00000221233_TCGA-IA-A40Y-01A_USP32_chr17_58329742_-_EXOSC5_chr19_41898885_length(amino acids)=181AA_start in transcript=230_stop in transcript=775 MAGVYGPAEVKVSKEIFNKATLEVILRPKIGLPGVAEKSRERLIRNTCEAVVLGTLHPRTSITVVLQVVSDAGSLLACCLNAACMALVDA GVPMRALFCGVACALDSDGTLVLDPTSKQEKEARAVLTFALDSVERKLLMSSTKGLYSDTELQQCLAAAQAASQHVFRFYRESLQRRYSK -------------------------------------------------------------- |
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Fusion Gene PPI Analysis for USP32-EXOSC5 |
| Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in |
| Protein-protein interactors with each fusion partner protein in wild-type (BIOGRID-3.4.160) |
| Hgene | Hgene's interactors | Tgene | Tgene's interactors |
| - Retained PPIs in in-frame fusion. |
| Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Still interaction with |
| - Lost PPIs in in-frame fusion. |
| Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Interaction lost with |
| - Retained PPIs, but lost function due to frame-shift fusion. |
| Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Interaction lost with |
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Related Drugs for USP32-EXOSC5 |
| Drugs targeting genes involved in this fusion gene. (DrugBank Version 5.1.8 2021-05-08) |
| Partner | Gene | UniProtAcc | DrugBank ID | Drug name | Drug activity | Drug type | Drug status |
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Related Diseases for USP32-EXOSC5 |
| Diseases associated with fusion partners. (DisGeNet 4.0) |
| Partner | Gene | Disease ID | Disease name | # pubmeds | Source |
| Hgene | USP32 | C4721610 | Carcinoma, Ovarian Epithelial | 1 | CTD_human |
| Tgene | EXOSC5 | C0024623 | Malignant neoplasm of stomach | 1 | CTD_human |
| Tgene | EXOSC5 | C0038356 | Stomach Neoplasms | 1 | CTD_human |
| Tgene | EXOSC5 | C0235874 | Disease Exacerbation | 1 | CTD_human |
| Tgene | EXOSC5 | C1708349 | Hereditary Diffuse Gastric Cancer | 1 | CTD_human |
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