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Center for Computational Systems Medicine
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Fusion Gene Summary

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Fusion Gene ORF analysis

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Fusion Genomic Features

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Fusion Protein Features

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Fusion Gene Sequence

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Fusion Gene PPI analysis

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Related Drugs

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Related Diseases

Fusion gene:VPS53-ZZEF1 (FusionGDB2 ID:97823)

Fusion Gene Summary for VPS53-ZZEF1

check button Fusion gene summary
Fusion gene informationFusion gene name: VPS53-ZZEF1
Fusion gene ID: 97823
HgeneTgene
Gene symbol

VPS53

ZZEF1

Gene ID

55275

23140

Gene nameVPS53 subunit of GARP complexzinc finger ZZ-type and EF-hand domain containing 1
SynonymsHCCS1|PCH2E|hVps53L|pp13624ZZZ4
Cytomap

17p13.3

17p13.2

Type of geneprotein-codingprotein-coding
Descriptionvacuolar protein sorting-associated protein 53 homologVPS53, GARP complex subunitzinc finger ZZ-type and EF-hand domain-containing protein 1zinc finger, ZZ-type with EF hand domain 1
Modification date2020031320200313
UniProtAcc..
Ensembl transtripts involved in fusion geneENST00000437048, ENST00000574029, 
ENST00000576149, ENST00000446250, 
ENST00000291074, ENST00000571805, 
ENST00000401468, 		ENST00000381638, 
ENST00000574474, 
Fusion gene scores* DoF score18 X 15 X 14=378012 X 15 X 6=1080
# samples 2415
** MAII scorelog2(24/3780*10)=-3.97727992349992
possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs).
DoF>8 and MAII<0		log2(15/1080*10)=-2.84799690655495
possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs).
DoF>8 and MAII<0
Context

PubMed: VPS53 [Title/Abstract] AND ZZEF1 [Title/Abstract] AND fusion [Title/Abstract]

Most frequent breakpointVPS53(600658)-ZZEF1(4027345), # samples:3
Anticipated loss of major functional domain due to fusion event.
* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

check button Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez
PartnerGeneGO IDGO termPubMed ID

check buttonFusion gene breakpoints across VPS53 (5'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

check buttonFusion gene breakpoints across ZZEF1 (3'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

check button Fusion gene information from two resources (ChiTars 5.0 and ChimerDB 4.0)
* All genome coordinats were lifted-over on hg19.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.
SourceDiseaseSampleHgeneHchrHbpHstrandTgeneTchrTbpTstrand
ChimerDB4LUADTCGA-55-8087-01AVPS53chr17

600658

-ZZEF1chr17

4027345

-
ChimerDB4LUADTCGA-55-8087-01AVPS53chr17

600658

-ZZEF1chr17

4027345

-
ChimerDB4LUADTCGA-55-8087-01AVPS53chr17

600658

-ZZEF1chr17

4027345

-


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Fusion Gene ORF analysis for VPS53-ZZEF1

check button Open reading frame (ORF) analsis of fusion genes based on Ensembl gene isoform structure.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.
ORFHenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrand
In-frameENST00000437048ENST00000381638VPS53chr17

600658

-ZZEF1chr17

4027345

-
5CDS-5UTRENST00000437048ENST00000574474VPS53chr17

600658

-ZZEF1chr17

4027345

-
intron-3CDSENST00000574029ENST00000381638VPS53chr17

600658

-ZZEF1chr17

4027345

-
intron-5UTRENST00000574029ENST00000574474VPS53chr17

600658

-ZZEF1chr17

4027345

-
intron-3CDSENST00000576149ENST00000381638VPS53chr17

600658

-ZZEF1chr17

4027345

-
intron-5UTRENST00000576149ENST00000574474VPS53chr17

600658

-ZZEF1chr17

4027345

-
5UTR-3CDSENST00000446250ENST00000381638VPS53chr17

600658

-ZZEF1chr17

4027345

-
5UTR-5UTRENST00000446250ENST00000574474VPS53chr17

600658

-ZZEF1chr17

4027345

-
In-frameENST00000291074ENST00000381638VPS53chr17

600658

-ZZEF1chr17

4027345

-
5CDS-5UTRENST00000291074ENST00000574474VPS53chr17

600658

-ZZEF1chr17

4027345

-
In-frameENST00000571805ENST00000381638VPS53chr17

600658

-ZZEF1chr17

4027345

-
5CDS-5UTRENST00000571805ENST00000574474VPS53chr17

600658

-ZZEF1chr17

4027345

-
In-frameENST00000401468ENST00000381638VPS53chr17

600658

-ZZEF1chr17

4027345

-
5CDS-5UTRENST00000401468ENST00000574474VPS53chr17

600658

-ZZEF1chr17

4027345

-

check buttonORFfinder result based on the fusion transcript sequence of in-frame fusion genes.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandSeq length
(transcript)		BP loci
(transcript)		Predicted start
(transcript)		Predicted stop
(transcript)		Seq length
(amino acids)
ENST00000437048VPS53chr17600658-ENST00000381638ZZEF1chr174027345-1140943214789632938
ENST00000291074VPS53chr17600658-ENST00000381638ZZEF1chr174027345-1139641913489502938
ENST00000571805VPS53chr17600658-ENST00000381638ZZEF1chr174027345-1139942213789532938
ENST00000401468VPS53chr17600658-ENST00000381638ZZEF1chr174027345-11262285088162938

check buttonDeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandNo-coding scoreCoding score
ENST00000437048ENST00000381638VPS53chr17600658-ZZEF1chr174027345-0.0017801420.9982198
ENST00000291074ENST00000381638VPS53chr17600658-ZZEF1chr174027345-0.0017467720.99825317
ENST00000571805ENST00000381638VPS53chr17600658-ZZEF1chr174027345-0.0017503970.9982496
ENST00000401468ENST00000381638VPS53chr17600658-ZZEF1chr174027345-0.0015120210.99848795

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Fusion Genomic Features for VPS53-ZZEF1


check buttonFusionAI prediction of the potential fusion gene breakpoint based on the pre-mature RNA sequence context (+/- 5kb of individual partner genes, total 20kb length sequence). FusionAI is a fusion gene breakpoint classifier based on convolutional neural network by comparing the fusion positive and negative sequence context of ~ 20K fusion gene data. From here, we can have the relative potentency of the 20K genomic sequence how individual sequnce will be likely used as the gene fusion breakpoints.
HgeneHchrHbpHstrandTgeneTchrTbpTstrand1-pp (fusion gene breakpoint)

check buttonDistribution of 44 human genomic features loci across 20kb length fusion breakpoint regions. We integrated a total of 44 different types of human genomic feature loci information across five big categories including virus integration sites, repeats, structural variants, chromatin states, and gene expression regulation. More details are in help page.

check buttonDistribution of 44 human genomic features loci across 20kb length fusion breakpoint regions that are ovelapped with the top 1% feature importance score regions. More details are in help page.

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Fusion Protein Features for VPS53-ZZEF1


check button Go to

FGviewer for the breakpoints of chr17:600658-chr17:4027345

.
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
FGviewer

check buttonMain function of each fusion partner protein. (from UniProt)
HgeneTgene
..
FUNCTION: Might normally function as a transcriptional repressor. EWS-fusion-proteins (EFPS) may play a role in the tumorigenic process. They may disturb gene expression by mimicking, or interfering with the normal function of CTD-POLII within the transcription initiation complex. They may also contribute to an aberrant activation of the fusion protein target genes.FUNCTION: Might normally function as a transcriptional repressor. EWS-fusion-proteins (EFPS) may play a role in the tumorigenic process. They may disturb gene expression by mimicking, or interfering with the normal function of CTD-POLII within the transcription initiation complex. They may also contribute to an aberrant activation of the fusion protein target genes.

check buttonRetention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at

download page


* Minus value of BPloci means that the break pointn is located before the CDS.
- In-frame and retained protein feature among the 13 regional features.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note
TgeneZZEF1chr17:600658chr17:4027345ENST00000381638055226_405118.02962.0DomainDOC
TgeneZZEF1chr17:600658chr17:4027345ENST000003816380551777_1825118.02962.0Zinc fingerZZ-type 1
TgeneZZEF1chr17:600658chr17:4027345ENST000003816380551826_1872118.02962.0Zinc fingerZZ-type 2

- In-frame and not-retained protein feature among the 13 regional features.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note
HgeneVPS53chr17:600658chr17:4027345ENST00000291074-41897_13895.0671.0Coiled coilOntology_term=ECO:0000255
HgeneVPS53chr17:600658chr17:4027345ENST00000437048-42297_13895.0833.0Coiled coilOntology_term=ECO:0000255
HgeneVPS53chr17:600658chr17:4027345ENST00000571805-41997_13895.0700.0Coiled coilOntology_term=ECO:0000255
TgeneZZEF1chr17:600658chr17:4027345ENST00000381638055111_146118.02962.0DomainEF-hand


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Fusion Gene Sequence for VPS53-ZZEF1


check button For in-frame fusion transcripts, we provide the fusion transcript sequences and fusion amino acid sequences. To have fusion amino acid sequence, we ran ORFfinder and chose the longest ORF among the all predicted ones.
>In-frame_ENST00000437048_ENST00000381638_TCGA-55-8087-01A_VPS53_chr17_600658_-_ZZEF1_chr17_4027345_length(transcript)=11409nt_BP=432nt
GAAGTGCCGGAGCGGCTGACAGAGCGGCTGACGGAGCCGGGCTCACCAGGTCGCTGCCGCGAGGGAGTTGCTGTGCTGGGGCCTGGGTGG
CGGCTGGAGGCCTGAGTTGGGCTCGCGGCGGGGGTCGGCAGGGGGCCGGGTGGCGGAATGATGGAGGAGGAGGAACTGGAGTTCGTGGAG
GAGCTGGAAGCCGTGCTGCAGCTCACGCCCGAGGTGCAGCTGGCCATCGAGCAGGTGTTTCCAAGCCAGGACCCTCTAGATCGAGCAGAT
TTCAATGCTGTTGAGTATATCAATACCCTGTTCCCAACCGAGCAATCTCTGGCGAACATAGACGAAGTCGTGAACAAAATTAGGCTGAAA
ATAAGGAGACTGGATGACAATATTCGAACTGTTGTAAGAGGTCAGACGAACGTGGGGCAGGATGGACGGCAAGCCTTTGCCCAGTTTGAT
GCTGAGGGTGATGGGACAGTTGATGCCGAGAACATGTTGGAGGCCCTCAAGAATTCCAGTGGAGCTAATCTTCAGGGGGAGCTGAGCCAC
ATCATCAGACAACTACAGGCCTGCTCTCTGGTTCCAGGTTTCACAGACATATTTTCAGAGTCGAAGGAGGGCCTTGATATTCACTCGTCA
ATGATACTGCGCTTCCTGCACCGCAATCGGCTCTCCAGCGCGGTGATGCCCTACCCGATGCTGGAGCACTGCAATAACATGTGCACCATG
CGGTCTTCCGTCCTGAAGGAGTCTCTGGATCAGCTGGTACAAAAGGAAAAGGAAAGCCCTGGAGATCTAACTAGAAGTCCAGAGATGGAT
AAACTCAAGTCAGTAGCAAAGTGCTATGCTTATATAGAAACATCCTCCAACTCGGCAGACATTGACAAGATGACAAATGGAGAAACCTCA
TCCTACTGGCAGTCAGATGGCAGTGCCTGTTCACACTGGATTCGTTTAAAAATGAAGCCAGATGTTGTGCTTAGGCACCTGTCCATTGCA
GTGGCTGCCACTGACCAGAGCTACATGCCACAGCAGGTGACAGTAGCTGTAGGGAGGAATGCCAGCGATCTTCAGGAAGTCCGAGATGTG
CACATCCCCAGCAATGTCACTGGCTATGTGACGCTGCTGGAAAATGCCAACGTCAGTCAGCTCTATGTCCAGATTAACATAAAGCGTTGT
CTTAGCGATGGCTGCGACACTAGAATTCATGGTCTCAGGGCTGTTGGCTTTCAGAGAGTTAAGAAGTCTGGGGTCTCAGTCTCAGATGCT
TCTGCAATATGGTATTGGTCTCTGCTGACATCTCTGGTGACGGCTTCTATGGAGACAAATCCCGCCTTTGTCCAGACAGTGCTGCACAAT
ACTCAGAAGGCGCTGCGGCACATGCCTCCACTCTCTCTCTCACCAGGATCTACAGATTTCTCAACTTTCCTCTCCCCTAATGTGCTGGAA
GAAGTGGACAGTTTCCTCATAAGGATAACTAGCTGCTGTTCTACCCCAGAGGTAGAACTGACTCTTCTGGCTTTTGCTCTCGCAAGAGGA
AGTGTTGCCAAAGTCATGAGCTCTCTATGCACCATCACTGACCATCTGGACACGCAGTATGATGCCTCATCCCTCATCTTGTCCATGGCG
TCAGTCAGACAGAACCTGCTCCTCAAATATGGTAAACCTCTCCAGTTGACTCTTCAGGCATGTGATGTCAAAGGAAAAGAAGATAAGTCT
GGACCCGAAAACCTCCTTGTTGAACCGTGGACAAGGGATGGTTTTCTTACGGAAACTGGAAAAACCAGAGCCAGCACTATTTTTTCTACC
GGAACTGAATCTGCCTTCCAAGTTACACAGATAAGAATTATGGTTCGACGTGGTGGCATTGGTGCCCAGTGTGGGTTGGTGTTTGCCTAT
AACTCATCTTCAGATAAATTTTGTGCGGAAGAACACTTCAAAAGGTTTGAAAAATATGACAAATGGAAGCTTCAGGAGCTCAGGCAATTT
GTAAAAAGCAGGATTGGTTGCTCATCTGATGACCTTGGAGAGGATGATCCTATTGGCTGGTTTGAACTGGAAGAAGAATGGGATGAAGCA
GATGTGAAGCTGCAACAGTGCAGAGTTGCCAAATATTTGATGGTGAAGTTCCTCTGCACCCGTCAGGAGTCAGCAGAGCGCTTGGGAGTG
CAAGGCTTGACCATCAGTGGGTACCTCCGGCCTGCAAGAGCAGAAGCAGAACAGAGCGTCACCTGTGCACACTGCAGAAAGGACACAGAG
GAGAGTGTCTGTGGGGCCACGTTGCTCCTCAGGACCCTTCAGTTTATCCAGCAGCTCGCCCATGACCTGGTGCAGCAGAAGGAAAGTGGC
TTAAAATATAAATCTTTTCTGGACTTCGCGGGTCTTGATTTGCAGATCTTCTGGAATTTTTACAGTAAATTAAAGCAAAACCCGAGGGAA
GAATGCGTCTCTGCCCAAACCCTGCTTCTGCAGCTACTCCAGAGCTGCTTCTCTGTGCTGCAGGGAGATGTACTGGCTGCTTCTGAGGAG
GAAAAGGCTCCAATCCAAAGCCCTAAAGGAGTAGAGGCTGCCAAGGAGCTGTACACACACTTGTGTGATGTGGTGGACAAGGTGGATGGA
GACTCTGTGCCCATGGAGATACTAAAACAAGAAGTCAGGAATACCCTTCTCAATGGGGCTGCCATCTTCTTTCCTAATCGACAGACCCGA
CGGAACCATCTCTTCACCATGATGAATGTCACCGAGCAGGAGCACAAGCAGTCCCTGCAGCTCACTTTCCGTTCACTGTGCACGTATTTT
AGTGACAAGGATCCAGGCGGCCTTCTTCTTTTACCTGAGAAGAACGACCTGGCCAAGATGAACATCAGTGAAGTCCTGGCGGTCATGGAC
ACTCTCGTCTCTGTTGCTGCTCGAGAGTGCGAGCTGTTAATGCTCAGTGGGGCCCCAGGGGAGGTGGGCTCTGTGCTCTTCTCCCTGTTC
TGGTCCGTCCAAGGCAGCCTGCTATCCTGGTGCTACCTGCAGCTGAAGAGCACGGACTCTGGAGCCAAAGATCTTGCCGTGGACCTTATT
GAAAAATATGTGGGCCAGTTTCTGGCAAGCATGAGAGCGATTTTGGAATCCCTTTTCTCACAGTACAGTGGAAAAACCATAGTAGAAAGA
TTATGTAACTCAGTGTTTTCAATGGCAGCTCGTCAACTGGTTATCTTCCTGCTGGACTTCTGCACTTTAGACATCCCACACTGCGTGCTC
TTGAGAGAGTTCAGCGTCCTCACAGAACTCCTGAAGAAGCTCTGTAGTGGCCCCGAAGGAGGACTGAGGAAGCTGGATGTTGAGACCTGG
CAACAGGAACAGCCTGTGGTGTTACATACGTGGACGAAGGAATCTGCCCACAACTATGAAAATAATTGCCATGAGGTATCCGTCTTTGTT
AGCCCAGGGGCAACCTATTTTGAAGTGGAATTCGATGACAGGTGTGAAACTGAAAAAAGGTATGATTATCTGGAATTTACCGACGCTAGA
GGTCGGAAAACACGCTATGACACAAAAGTTGGCACTGATAAATGGCCCAAGAAAGTGACCTTCAAGGCCGGTCCTCGGTTGCAGTTCCTC
TTTCACTCTGACAGCAGTCACAACGAATGGGGCTACAAATTCACTGTCACTGCCTGTGGGCTGCCCGATGTTGCCGTGTCTTGGGGGCTG
GATTTACAGCTCCTCGTCTCCCGGCTGATGGGACGCCTGGCTTCCCAGTGCATGGCGCTCAAGTCTGTGCGCCAGTTAGGAAGTAACATG
GTAGTACCTCAGGCAAAAATGGCATTAGTCCTAAGCTCCCCACTGTGGAAACCTGTCTTCAGGCATCAGGTTTGTCCAGAGTTGGAATTA
GAAGCAAGCTGGCCCACTCACCCACACCGGAATAGTAAGGAGGTTAAGAACATTCCTGACGACCCCTGCCGCCATTTTCTTCTTGATTTT
GCCCAGTCAGAGCCTGCTCAGAACTTCTGTGGGCCATATTCAGAACTTTTCAAAGGATTCATACAGGCATGTAGAAAACAGGCCCCAAAG
ACAGATATAGTTGCTGGTTCCACTATTGATCAAGCTGTGAACGCCACCTTTGCTGCTCTGGTGTATCGCACTCCAGATTTATATGAGAAG
CTGCAAAAATATGTCAACAGTGGGGGCAAAATAGCCCTGAGTGAAGAGTTTGCCCAGGTTTATTCCTTGGCAGATGGGATTCGAATATGG
ATGTTAGAGATGAAGCAGAAGTCCCTGATGAGCCTGGGGAATGAAGCAGAAGAAAAACATAGTTCAGAAGCTACTGAGGTGAACCCTGAG
AGCCTGGCAAAAGAGTGCATTGAGAAGAGTCTTCTATTACTAAAATTTCTGCCCACGGGCATAAGTTCAAAAGAAAGCTGCGAAAAGTTG
GAGACTGCTGATGAAACCAGTCATCTCCAGCCACTCAACAAGCGTCAGAGGACAAGCTCTGTGGTGGAAGAGCATTTCCAAGCCTCAGTA
TCTCCCACTGAAGCCGCACCCCCTGCCACAGGAGACCAGAGTCCTGGCCTGGGCACCCAGCCAAAGCTGCCATCCAGCAGTGGCCTTCCT
GCTGCAGACGTGTCCCCTGCCACAGCTGAAGAGCCCTTGTCACCTTCCACACCCACCCGCCGGCCTCCCTTCACCCGAGGGCGACTCCGG
CTGCTCTCCTTTCGATCCATGGAGGAGGCCAGACTGGTGCCCACAGTGAAAGAGAAATACCCTGTGCTGAAGGACGTCATGGACTTCATT
AAGGATCAGTCGCTCTCGCACAGGAGTGTTGTGAAGGTTCTTTCCCTGAGGAAAGCCCAGGCCCAGAGCATCCTGGAAGTCCTGAAGATA
ACTCAGCACTGTGCAGAATCCCTTGGGCAGCCCCACTGCTTCCATCCACCTTTCATACTTTTCCTGTTGGAACTTCTGACCTGTCAGAAA
GATTTTACCAATTATTTTGGACACCTGGAAGGCTGTGGTGCTGATCTACACAAAGAAATTCGAGACACTTACTATCAACTTGTTCTGTTT
TTGGTCAAAGCAGTTAAAGGATTTAGTAGCCTAAATGACAGGTCCTTGCTCCCTGCCTTATCCTGTGTTCAGACAGCCCTGCTTCATCTT
TTGGATATGGGCTGGGAACCCAATGATCTCGCCTTCTTTGTTGATATTCAGTTACCAGATCTCCTCATGAAAATGTCACAGGAGAATATA
AGTGTCCATGACAGTGTGATCAGCCAATGGAGTGAAGAAGATGAGCTTGCTGATGCCAAGCAGAATTCAGAATGGATGGATGAGTGTCAG
GATGGCATGTTTGAGGCCTGGTATGAAAAAATAGCCCAGGAAGATCCAGAGAAGCAGAGGAAAATGCACATGTTCATTGCTCGCTACTGT
GACCTGTTAAATGTGGACATCTCTTGTGATGGGTGTGATGAGATTGCCCCCTGGCATCGATACCGCTGTCTGCAGTGCAGCGACATGGAT
CTCTGCAAAACTTGCTTCCTAGGTGGGGTGAAGCCTGAGGGCCACGGAGACGACCATGAAATGGTCAACATGGAGTTTACCTGTGACCAC
TGCCAGGGTTTGATCATAGGCCGGAGGATGAACTGCAATGTTTGCGATGACTTTGATCTTTGCTACGGATGCTATGCAGCGAAGAAATAC
TCCTACGGCCATTTGCCTACCCACAGCATCACGGCCCACCCAATGGTAACCATTCGGATCAGTGACCGGCAGAGGCTCATCCAGCCATAT
ATCCATAACTACTCCTGGCTGCTCTTTGCTGCCCTGGCTCTCTATAGCGCCCACCTGGCCAGTGCAGAGGATGTGGATGGGGAGAAGCTG
GACCCCCAGACGCGCAGCAGTGCCACCACCCTGCGGAGCCAGTGCATGCAGCTCGTCGGGGACTGTCTGATGAAGGCTCATCAGGGAAAA
GGCCTTAAAGCTCTAGCTTTGCTGGGTGTATTGCCAGATGGGGACTCGAGCCTAGAAGATCAGGCCCTACCAGTCACTGTGCCCACCGGA
GCGTCAGAGGAGCAGCTAGAGAAGAAAGCTGTCCAGGGTGCTGAGCTGTCAGAAGCAGGCAATGGAAAGAGAGCTGTTCATGAGGAAATC
AGACCTGTAGATTTCAAGCAGAGAAATAAGGCAGATAAAGGTGTATCATTATCGAAGGATCCTTCATGCCAGACCCAAATTTCAGATTCA
CCTGCAGATGCTAGCCCACCTACAGGACTTCCAGATGCTGAAGATTCAGAAGTGTCATCTCAGAAGCCCATAGAGGAAAAAGCAGTTACT
CCAAGCCCTGAGCAAGTGTTTGCTGAGTGTTCCCAGAAGAGGATTTTGGGATTACTAGCAGCCATGTTACCTCCCTTAAAGTCGGGCCCC
ACGGTTCCCCTGATAGACCTGGAGCACGTCCTTCCACTCATGTTTCAGGTTGTCATCTCAAACGCAGGCCACCTGAATGAAACCTACCAT
CTCACCCTGGGTCTTCTCGGCCAGTTAATTATCCGTCTTTTGCCAGCAGAGGTAGACGCCGCAGTGATCAAAGTCCTCTCAGCCAAACAC
AACCTGTTTGCTGCAGGGGACAGTTCCATTGTGCCAGATGGCTGGAAAACCACCCACCTGCTCTTTAGCCTGGGAGCTGTGTGTCTGGAC
AGCCGGGTGGGCTTGGACTGGGCGTGCTCCATGGCAGAGATCCTGCGGTCACTCAACAGTGCCCCACTGTGGCGTGATGTCATTGCCACC
TTCACAGACCACTGCATCAAGCAGCTGCCATTCCAGCTGAAGCACACCAACATCTTCACCCTGCTCGTGCTGGTTGGCTTCCCCCAGGTC
CTCTGTGTGGGAACCCGCTGCGTTTATATGGATAATGCCAATGAACCCCATAATGTGATCATCTTGAAGCACTTTACTGAGAAGAACAGG
GCTGTGATTGTTGATGTCAAAACTCGGAAGAGGAAAACAGTGAAGGACTACCAGCTGGTCCAGAAGGGAGGAGGACAAGAGTGTGGTGAC
TCTCGGGCCCAGCTGAGCCAGTACTCCCAGCACTTTGCCTTTATCGCCAGTCACCTTCTGCAAAGCAGCATGGACAGCCATTGTCCCGAG
GCAGTAGAAGCAACTTGGGTCCTGTCCCTGGCCCTGAAAGGATTGTATAAAACACTAAAGGCTCACGGTTTTGAGGAGATCCGTGCTACT
TTCCTTCAGACCGATTTGCTGAAGTTGCTGGTGAAAAAGTGCAGCAAAGGGACTGGCTTTAGTAAAACGTGGCTCCTCCGGGACCTGGAA
ATTTTGTCCATCATGCTGTACTCCTCAAAAAAGGAGATCAACGCTTTGGCTGAGCACGGAGACCTAGAGCTGGATGAGCGAGGGGACCGA
GAGGAAGAGGTGGAACGGCCAGTCAGCAGCCCTGGCGACCCAGAGCAGAAAAAGCTGGACCCCCTTGAGGGCCTGGATGAGCCCACCAGA
ATATGTTTCTTGATGGCTCATGATGCCCTCAATGCCCCTCTGCACATTCTCCGGGCCATATACGAACTGCAGATGAAAAAGACCGATTAT
TTCTTCCTGGAGGTTCAGAAGAGGTTTGATGGTGATGAGCTCACCACAGATGAAAGGATACGGTCCCTGGCTCAGCGGTGGCAGCCCAGT
AAGAGTCTGAGGCTGGAAGAACAGAGCGCCAAAGCTGTGGATACAGACATGATTATCCTGCCATGCTTGTCCCGGCCTGCACGCTGTGAC
CAAGCCACTGCTGAATCGAACCCTGTGACCCAGAAGCTGATCTCCAGCACAGAGAGCGAACTGCAGCAGAGCTATGCCAAGCAGCGCCGT
AGCAAGAGCGCCGCCCTCCTGCACAAGGAGCTGAACTGCAAGAGTAAGAGGGCTGTCCGGGACTACCTCTTCCGAGTGAACGAGGCCACA
GCTGTCCTGTACGCCCGCCACGTGCTTGCATCCCTGCTCGCCGAGTGGCCTAGCCACGTGCCAGTGAGCGAGGACATCCTGGAGCTCAGT
GGCCCTGCCCATATGACCTACATTTTGGATATGTTCATGCAGCTGGAAGAAAAGCATGAGTGGGAGAAGATCCTGCAGAAAGTGCTCCAG
GGCTGCCGAGAGGACATGCTGGGGACCATGGCCCTGGCTGCATGCCAGTTCATGGAGGAGCCAGGAATGGAGGTGCAAGTGAGGGAGTCG
AAACACCCGTATAACAACAACACCAACTTCGAGGATAAAGTTCACATTCCTGGTGCCATCTACCTCTCAATCAAATTCGACTCTCAGTGC
AACACAGAGGAGGGCTGTGACGAGTTAGCCATGTCCAGCAGCAGTGACTTCCAGCAAGACCGACACAGCTTCAGCGGGTCTCAGCAGAAG
TGGAAAGATTTTGAACTTCCAGGAGACACTCTGTATTACCGCTTCACCTCCGACATGAGCAACACCGAGTGGGGCTACAGATTCACCGTG
ACGGCCGGACACCTGGGGCGGTTCCAGACAGGATTCGAGATTTTGAAGCAGATGTTGTCAGAAGAAAGGGTCGTGCCTCATCTCCCATTG
GCAAAAATTTGGGAATGGCTGGTGGGCGTGGCCTGTCGCCAGACTGGCCATCAACGATTAAAAGCCATCCACTTACTTCTGAGGATTGTG
CGATGCTGCGGCCACAGTGACCTGTGTGACCTTGCGCTGTTGAAGCCCCTGTGGCAGCTCTTTACCCACATGGAGTACGGCCTGTTTGAG
GACGTGACGCAGCCCGGCATCCTCCTTCCCCTGCATCGTGCCCTCACTGAGCTCTTCTTCGTCACCGAGAACCGTGCCCAGGAGCTTGGC
GTGCTGCAGGATTACCTGCTGGCCCTAACCACGGACGACCACCTTCTCCGCTGTGCGGCACAGGCTCTGCAGAACATTGCTGCCATCAGC
CTGGCCATCAACTACCCAAACAAGGCCACCCGCCTCTGGAATGTGGAGTGTTAGCCCTTGGTGGGGCGTGCATGGGACTAGTTCATCTGC
CACAGGGATTTTAGAGCAGACATCTAACCTCATTCAGGAAAACTCCTGTAGCGCCAGTGCCCAGCTCTCCTTGAGCTGACCACTCCAGTT
AGGATGCCAAGCAGCCACGTCTCCAAGAGCTCCCGTGCGTAGGCTGGACACAAGCACAGGCTGTAGCATGGTGAAAATAAGCCAAGCAGT
GCAGAATGCCTCAGAAAGGGTGGGCAGGGGGCCCTTAAGAAGGTTCAGAGACCAGCCTTCTCCAGAGGCTGTCACTGCAGGAGCCGTGGG
CCTGGGAAGACTTGGAAGCGGCCTCTCTCAACTGGTTTCTGTCTCCGTGGAGCTGGAACTGCCTGCACTTGCCTTCAGAGGGAGGCACAG
TCCACCCAGATCCACCTTTCCAGCAAGACCCCCAGTGGCTGCCCAGCCTGGGAGCACCTCTTTGCTTTTCACACCAAACCAAAACTGGCG
AGAGCCCCTCCTAGCCACCAGTGATCCCCAAGCATCCAGTACAGAACCAGGCATCGAGCTAGCTCCCTGCACGGCCGCACCCTCCCAGAG
AACTCCTTGAGGAGAACAAGTGCCCTTGGGGACAGCCGGCAGGCGCCCCTGTACGTCTGCTCATGCACCAGGCAGCACAGCCGCAGTTCC
TCAGTTGTTGTTTTGACATATTTCAGTTTCCACCTCACGTTTTTAGAGCAGAACCACACTGTCTCCCTGGAGGGGCTCGAGGGCATGACC
GGGGACTGACCATTCTGTGAAAGGAGCAGAATGTGAGGAGCACGCGTGAGCTTATGTACCGTGAAGATGATCAGAGGATATCTTATTTTA
AGAGTAAAAACCCACATAATTTTATTTCTGCTTGATAGTCATGGTAGTCTGTCATACCCACCTCTGGGACTCTGCGTGGCTGTTTGGCTG
TCACTTGTAGCAATAACGACATTAGTTCTAGTCAGTGCTGTTTTACATTTTTCTTTTGATGGGTTTAGTCTTGCCCTGGAGTGCCGATGA
TGATTCTCCCTCCAGAGCCACGCTTGGGAACATGAAGCAAGTCTGGCGTGTGGGCTGCGTGCCGGCCTTAGTGGGACCCGTGGGGTTGGA
GCATGCCTTTAGGGGCAGTGTCTGGGCCGAAGCACGTCCCACCACACAGTGCCAGAGCCAGAGAAGGGGCCCCACCACCAAGGCCAAGCT
TGACCAGGTCAGCATTGCCATGGCCCAGTGTGCCCCGTGGCCTCTGAAGATCCCTCTGTGCAGGGTCTGCAGGGATCTGGATTGCAAGGG
CCCAAGTCTGCAGGTCTGGAAGCATCTTCCTATAAGAGCACTTTCGCCTTCTGGGTCAGGACTCCAAGGTGCAGCGGGCTTCACAGCCCT
ACAATTGGGTTCTCAGCTAAGCCCCAGAGTTCTGGTAGAACCATCCCGGGGCGGGTGGAGGGTGGGATTTAAGGGAGACGGGAACACATG
GGGCAGGTCCTGGAACTTGGTGGCCTGAGGACTGAGGCCATTGCCCTGGTGGAAAGGCCTGGCCTGGTTCCTGTGGCTTGGGACCTGAAT
AGGCAGGTGCTGCTGGCTCCGTAGAAACCCTTTTCCCATCTTTTGCTCTTTGCCAAACCTACCTTGCTTTGGGAGCTGCCTGCACCACCC
CAGAGAAGGCCCCACCTTCTTCATCCCTCAGACCCGAGGAGGCCTCCCAGTAAGGAGTTTCCCAAGAGGGGACTCACAGGAAACAAGTCT
TAGTGCTTGGGAGGGAGGCCCCGCTGCGTGCTCAGACTCACAGCCAACCTGGAAGGTAGACGAGATAGCGCCACCCACGCCCCTCCACAC
CCCAGACTCCGAGTAAAGCGGGCGGTAGGGCCGGAGTCACCTCCCCTATGGCAGTGGCCGCCGCTGTACTCCATCCTCCCGTCAGGAAGA
TCAGCTGTAAATAAACGCTGGGCTCCCCAGAGCACCTGTCCGCCCACTGCCCTTGCTGTTCTGGGATCTTCGCTGCAGTTCACGGGAAAC
AAGCCTGAGTCCGCTCGCACCCGCGGCTGCTCTCCCGGCTCGGCCCGGCCGCCTCTGTCTCCGGCCACCGGGTGGCGCTGCCGAGCCAGA
GCCGCCGCGTCCCGGCGCTTTCCAGGAGCCCCAGGCCCGGAGGAGGCGAAGCCCGCAGAGCAAAGGTGGAAACACGTGCCTACGCTGTAA
AGAAATCCTGTTCCAGAGCATACCTGTTGTACAAACAGACACTGTTCCTAACGAGAGGAGTGACGTATTTTCATCACCGTTTTTAATTTG
TTTTCTTACGGGTTTACGATTTTGAATTTTTCTTATTTGGTTGAAAGAATTTTGATTCTATCAGCCTGAGTGAGTTCAGCCTGTAAAAAG

>In-frame_ENST00000437048_ENST00000381638_TCGA-55-8087-01A_VPS53_chr17_600658_-_ZZEF1_chr17_4027345_length(amino acids)=2938AA_start in transcript=147_stop in transcript=8963
MMEEEELEFVEELEAVLQLTPEVQLAIEQVFPSQDPLDRADFNAVEYINTLFPTEQSLANIDEVVNKIRLKIRRLDDNIRTVVRGQTNVG
QDGRQAFAQFDAEGDGTVDAENMLEALKNSSGANLQGELSHIIRQLQACSLVPGFTDIFSESKEGLDIHSSMILRFLHRNRLSSAVMPYP
MLEHCNNMCTMRSSVLKESLDQLVQKEKESPGDLTRSPEMDKLKSVAKCYAYIETSSNSADIDKMTNGETSSYWQSDGSACSHWIRLKMK
PDVVLRHLSIAVAATDQSYMPQQVTVAVGRNASDLQEVRDVHIPSNVTGYVTLLENANVSQLYVQINIKRCLSDGCDTRIHGLRAVGFQR
VKKSGVSVSDASAIWYWSLLTSLVTASMETNPAFVQTVLHNTQKALRHMPPLSLSPGSTDFSTFLSPNVLEEVDSFLIRITSCCSTPEVE
LTLLAFALARGSVAKVMSSLCTITDHLDTQYDASSLILSMASVRQNLLLKYGKPLQLTLQACDVKGKEDKSGPENLLVEPWTRDGFLTET
GKTRASTIFSTGTESAFQVTQIRIMVRRGGIGAQCGLVFAYNSSSDKFCAEEHFKRFEKYDKWKLQELRQFVKSRIGCSSDDLGEDDPIG
WFELEEEWDEADVKLQQCRVAKYLMVKFLCTRQESAERLGVQGLTISGYLRPARAEAEQSVTCAHCRKDTEESVCGATLLLRTLQFIQQL
AHDLVQQKESGLKYKSFLDFAGLDLQIFWNFYSKLKQNPREECVSAQTLLLQLLQSCFSVLQGDVLAASEEEKAPIQSPKGVEAAKELYT
HLCDVVDKVDGDSVPMEILKQEVRNTLLNGAAIFFPNRQTRRNHLFTMMNVTEQEHKQSLQLTFRSLCTYFSDKDPGGLLLLPEKNDLAK
MNISEVLAVMDTLVSVAARECELLMLSGAPGEVGSVLFSLFWSVQGSLLSWCYLQLKSTDSGAKDLAVDLIEKYVGQFLASMRAILESLF
SQYSGKTIVERLCNSVFSMAARQLVIFLLDFCTLDIPHCVLLREFSVLTELLKKLCSGPEGGLRKLDVETWQQEQPVVLHTWTKESAHNY
ENNCHEVSVFVSPGATYFEVEFDDRCETEKRYDYLEFTDARGRKTRYDTKVGTDKWPKKVTFKAGPRLQFLFHSDSSHNEWGYKFTVTAC
GLPDVAVSWGLDLQLLVSRLMGRLASQCMALKSVRQLGSNMVVPQAKMALVLSSPLWKPVFRHQVCPELELEASWPTHPHRNSKEVKNIP
DDPCRHFLLDFAQSEPAQNFCGPYSELFKGFIQACRKQAPKTDIVAGSTIDQAVNATFAALVYRTPDLYEKLQKYVNSGGKIALSEEFAQ
VYSLADGIRIWMLEMKQKSLMSLGNEAEEKHSSEATEVNPESLAKECIEKSLLLLKFLPTGISSKESCEKLETADETSHLQPLNKRQRTS
SVVEEHFQASVSPTEAAPPATGDQSPGLGTQPKLPSSSGLPAADVSPATAEEPLSPSTPTRRPPFTRGRLRLLSFRSMEEARLVPTVKEK
YPVLKDVMDFIKDQSLSHRSVVKVLSLRKAQAQSILEVLKITQHCAESLGQPHCFHPPFILFLLELLTCQKDFTNYFGHLEGCGADLHKE
IRDTYYQLVLFLVKAVKGFSSLNDRSLLPALSCVQTALLHLLDMGWEPNDLAFFVDIQLPDLLMKMSQENISVHDSVISQWSEEDELADA
KQNSEWMDECQDGMFEAWYEKIAQEDPEKQRKMHMFIARYCDLLNVDISCDGCDEIAPWHRYRCLQCSDMDLCKTCFLGGVKPEGHGDDH
EMVNMEFTCDHCQGLIIGRRMNCNVCDDFDLCYGCYAAKKYSYGHLPTHSITAHPMVTIRISDRQRLIQPYIHNYSWLLFAALALYSAHL
ASAEDVDGEKLDPQTRSSATTLRSQCMQLVGDCLMKAHQGKGLKALALLGVLPDGDSSLEDQALPVTVPTGASEEQLEKKAVQGAELSEA
GNGKRAVHEEIRPVDFKQRNKADKGVSLSKDPSCQTQISDSPADASPPTGLPDAEDSEVSSQKPIEEKAVTPSPEQVFAECSQKRILGLL
AAMLPPLKSGPTVPLIDLEHVLPLMFQVVISNAGHLNETYHLTLGLLGQLIIRLLPAEVDAAVIKVLSAKHNLFAAGDSSIVPDGWKTTH
LLFSLGAVCLDSRVGLDWACSMAEILRSLNSAPLWRDVIATFTDHCIKQLPFQLKHTNIFTLLVLVGFPQVLCVGTRCVYMDNANEPHNV
IILKHFTEKNRAVIVDVKTRKRKTVKDYQLVQKGGGQECGDSRAQLSQYSQHFAFIASHLLQSSMDSHCPEAVEATWVLSLALKGLYKTL
KAHGFEEIRATFLQTDLLKLLVKKCSKGTGFSKTWLLRDLEILSIMLYSSKKEINALAEHGDLELDERGDREEEVERPVSSPGDPEQKKL
DPLEGLDEPTRICFLMAHDALNAPLHILRAIYELQMKKTDYFFLEVQKRFDGDELTTDERIRSLAQRWQPSKSLRLEEQSAKAVDTDMII
LPCLSRPARCDQATAESNPVTQKLISSTESELQQSYAKQRRSKSAALLHKELNCKSKRAVRDYLFRVNEATAVLYARHVLASLLAEWPSH
VPVSEDILELSGPAHMTYILDMFMQLEEKHEWEKILQKVLQGCREDMLGTMALAACQFMEEPGMEVQVRESKHPYNNNTNFEDKVHIPGA
IYLSIKFDSQCNTEEGCDELAMSSSSDFQQDRHSFSGSQQKWKDFELPGDTLYYRFTSDMSNTEWGYRFTVTAGHLGRFQTGFEILKQML
SEERVVPHLPLAKIWEWLVGVACRQTGHQRLKAIHLLLRIVRCCGHSDLCDLALLKPLWQLFTHMEYGLFEDVTQPGILLPLHRALTELF

--------------------------------------------------------------
>In-frame_ENST00000291074_ENST00000381638_TCGA-55-8087-01A_VPS53_chr17_600658_-_ZZEF1_chr17_4027345_length(transcript)=11396nt_BP=419nt
GGCTGACAGAGCGGCTGACGGAGCCGGGCTCACCAGGTCGCTGCCGCGAGGGAGTTGCTGTGCTGGGGCCTGGGTGGCGGCTGGAGGCCT
GAGTTGGGCTCGCGGCGGGGGTCGGCAGGGGGCCGGGTGGCGGAATGATGGAGGAGGAGGAACTGGAGTTCGTGGAGGAGCTGGAAGCCG
TGCTGCAGCTCACGCCCGAGGTGCAGCTGGCCATCGAGCAGGTGTTTCCAAGCCAGGACCCTCTAGATCGAGCAGATTTCAATGCTGTTG
AGTATATCAATACCCTGTTCCCAACCGAGCAATCTCTGGCGAACATAGACGAAGTCGTGAACAAAATTAGGCTGAAAATAAGGAGACTGG
ATGACAATATTCGAACTGTTGTAAGAGGTCAGACGAACGTGGGGCAGGATGGACGGCAAGCCTTTGCCCAGTTTGATGCTGAGGGTGATG
GGACAGTTGATGCCGAGAACATGTTGGAGGCCCTCAAGAATTCCAGTGGAGCTAATCTTCAGGGGGAGCTGAGCCACATCATCAGACAAC
TACAGGCCTGCTCTCTGGTTCCAGGTTTCACAGACATATTTTCAGAGTCGAAGGAGGGCCTTGATATTCACTCGTCAATGATACTGCGCT
TCCTGCACCGCAATCGGCTCTCCAGCGCGGTGATGCCCTACCCGATGCTGGAGCACTGCAATAACATGTGCACCATGCGGTCTTCCGTCC
TGAAGGAGTCTCTGGATCAGCTGGTACAAAAGGAAAAGGAAAGCCCTGGAGATCTAACTAGAAGTCCAGAGATGGATAAACTCAAGTCAG
TAGCAAAGTGCTATGCTTATATAGAAACATCCTCCAACTCGGCAGACATTGACAAGATGACAAATGGAGAAACCTCATCCTACTGGCAGT
CAGATGGCAGTGCCTGTTCACACTGGATTCGTTTAAAAATGAAGCCAGATGTTGTGCTTAGGCACCTGTCCATTGCAGTGGCTGCCACTG
ACCAGAGCTACATGCCACAGCAGGTGACAGTAGCTGTAGGGAGGAATGCCAGCGATCTTCAGGAAGTCCGAGATGTGCACATCCCCAGCA
ATGTCACTGGCTATGTGACGCTGCTGGAAAATGCCAACGTCAGTCAGCTCTATGTCCAGATTAACATAAAGCGTTGTCTTAGCGATGGCT
GCGACACTAGAATTCATGGTCTCAGGGCTGTTGGCTTTCAGAGAGTTAAGAAGTCTGGGGTCTCAGTCTCAGATGCTTCTGCAATATGGT
ATTGGTCTCTGCTGACATCTCTGGTGACGGCTTCTATGGAGACAAATCCCGCCTTTGTCCAGACAGTGCTGCACAATACTCAGAAGGCGC
TGCGGCACATGCCTCCACTCTCTCTCTCACCAGGATCTACAGATTTCTCAACTTTCCTCTCCCCTAATGTGCTGGAAGAAGTGGACAGTT
TCCTCATAAGGATAACTAGCTGCTGTTCTACCCCAGAGGTAGAACTGACTCTTCTGGCTTTTGCTCTCGCAAGAGGAAGTGTTGCCAAAG
TCATGAGCTCTCTATGCACCATCACTGACCATCTGGACACGCAGTATGATGCCTCATCCCTCATCTTGTCCATGGCGTCAGTCAGACAGA
ACCTGCTCCTCAAATATGGTAAACCTCTCCAGTTGACTCTTCAGGCATGTGATGTCAAAGGAAAAGAAGATAAGTCTGGACCCGAAAACC
TCCTTGTTGAACCGTGGACAAGGGATGGTTTTCTTACGGAAACTGGAAAAACCAGAGCCAGCACTATTTTTTCTACCGGAACTGAATCTG
CCTTCCAAGTTACACAGATAAGAATTATGGTTCGACGTGGTGGCATTGGTGCCCAGTGTGGGTTGGTGTTTGCCTATAACTCATCTTCAG
ATAAATTTTGTGCGGAAGAACACTTCAAAAGGTTTGAAAAATATGACAAATGGAAGCTTCAGGAGCTCAGGCAATTTGTAAAAAGCAGGA
TTGGTTGCTCATCTGATGACCTTGGAGAGGATGATCCTATTGGCTGGTTTGAACTGGAAGAAGAATGGGATGAAGCAGATGTGAAGCTGC
AACAGTGCAGAGTTGCCAAATATTTGATGGTGAAGTTCCTCTGCACCCGTCAGGAGTCAGCAGAGCGCTTGGGAGTGCAAGGCTTGACCA
TCAGTGGGTACCTCCGGCCTGCAAGAGCAGAAGCAGAACAGAGCGTCACCTGTGCACACTGCAGAAAGGACACAGAGGAGAGTGTCTGTG
GGGCCACGTTGCTCCTCAGGACCCTTCAGTTTATCCAGCAGCTCGCCCATGACCTGGTGCAGCAGAAGGAAAGTGGCTTAAAATATAAAT
CTTTTCTGGACTTCGCGGGTCTTGATTTGCAGATCTTCTGGAATTTTTACAGTAAATTAAAGCAAAACCCGAGGGAAGAATGCGTCTCTG
CCCAAACCCTGCTTCTGCAGCTACTCCAGAGCTGCTTCTCTGTGCTGCAGGGAGATGTACTGGCTGCTTCTGAGGAGGAAAAGGCTCCAA
TCCAAAGCCCTAAAGGAGTAGAGGCTGCCAAGGAGCTGTACACACACTTGTGTGATGTGGTGGACAAGGTGGATGGAGACTCTGTGCCCA
TGGAGATACTAAAACAAGAAGTCAGGAATACCCTTCTCAATGGGGCTGCCATCTTCTTTCCTAATCGACAGACCCGACGGAACCATCTCT
TCACCATGATGAATGTCACCGAGCAGGAGCACAAGCAGTCCCTGCAGCTCACTTTCCGTTCACTGTGCACGTATTTTAGTGACAAGGATC
CAGGCGGCCTTCTTCTTTTACCTGAGAAGAACGACCTGGCCAAGATGAACATCAGTGAAGTCCTGGCGGTCATGGACACTCTCGTCTCTG
TTGCTGCTCGAGAGTGCGAGCTGTTAATGCTCAGTGGGGCCCCAGGGGAGGTGGGCTCTGTGCTCTTCTCCCTGTTCTGGTCCGTCCAAG
GCAGCCTGCTATCCTGGTGCTACCTGCAGCTGAAGAGCACGGACTCTGGAGCCAAAGATCTTGCCGTGGACCTTATTGAAAAATATGTGG
GCCAGTTTCTGGCAAGCATGAGAGCGATTTTGGAATCCCTTTTCTCACAGTACAGTGGAAAAACCATAGTAGAAAGATTATGTAACTCAG
TGTTTTCAATGGCAGCTCGTCAACTGGTTATCTTCCTGCTGGACTTCTGCACTTTAGACATCCCACACTGCGTGCTCTTGAGAGAGTTCA
GCGTCCTCACAGAACTCCTGAAGAAGCTCTGTAGTGGCCCCGAAGGAGGACTGAGGAAGCTGGATGTTGAGACCTGGCAACAGGAACAGC
CTGTGGTGTTACATACGTGGACGAAGGAATCTGCCCACAACTATGAAAATAATTGCCATGAGGTATCCGTCTTTGTTAGCCCAGGGGCAA
CCTATTTTGAAGTGGAATTCGATGACAGGTGTGAAACTGAAAAAAGGTATGATTATCTGGAATTTACCGACGCTAGAGGTCGGAAAACAC
GCTATGACACAAAAGTTGGCACTGATAAATGGCCCAAGAAAGTGACCTTCAAGGCCGGTCCTCGGTTGCAGTTCCTCTTTCACTCTGACA
GCAGTCACAACGAATGGGGCTACAAATTCACTGTCACTGCCTGTGGGCTGCCCGATGTTGCCGTGTCTTGGGGGCTGGATTTACAGCTCC
TCGTCTCCCGGCTGATGGGACGCCTGGCTTCCCAGTGCATGGCGCTCAAGTCTGTGCGCCAGTTAGGAAGTAACATGGTAGTACCTCAGG
CAAAAATGGCATTAGTCCTAAGCTCCCCACTGTGGAAACCTGTCTTCAGGCATCAGGTTTGTCCAGAGTTGGAATTAGAAGCAAGCTGGC
CCACTCACCCACACCGGAATAGTAAGGAGGTTAAGAACATTCCTGACGACCCCTGCCGCCATTTTCTTCTTGATTTTGCCCAGTCAGAGC
CTGCTCAGAACTTCTGTGGGCCATATTCAGAACTTTTCAAAGGATTCATACAGGCATGTAGAAAACAGGCCCCAAAGACAGATATAGTTG
CTGGTTCCACTATTGATCAAGCTGTGAACGCCACCTTTGCTGCTCTGGTGTATCGCACTCCAGATTTATATGAGAAGCTGCAAAAATATG
TCAACAGTGGGGGCAAAATAGCCCTGAGTGAAGAGTTTGCCCAGGTTTATTCCTTGGCAGATGGGATTCGAATATGGATGTTAGAGATGA
AGCAGAAGTCCCTGATGAGCCTGGGGAATGAAGCAGAAGAAAAACATAGTTCAGAAGCTACTGAGGTGAACCCTGAGAGCCTGGCAAAAG
AGTGCATTGAGAAGAGTCTTCTATTACTAAAATTTCTGCCCACGGGCATAAGTTCAAAAGAAAGCTGCGAAAAGTTGGAGACTGCTGATG
AAACCAGTCATCTCCAGCCACTCAACAAGCGTCAGAGGACAAGCTCTGTGGTGGAAGAGCATTTCCAAGCCTCAGTATCTCCCACTGAAG
CCGCACCCCCTGCCACAGGAGACCAGAGTCCTGGCCTGGGCACCCAGCCAAAGCTGCCATCCAGCAGTGGCCTTCCTGCTGCAGACGTGT
CCCCTGCCACAGCTGAAGAGCCCTTGTCACCTTCCACACCCACCCGCCGGCCTCCCTTCACCCGAGGGCGACTCCGGCTGCTCTCCTTTC
GATCCATGGAGGAGGCCAGACTGGTGCCCACAGTGAAAGAGAAATACCCTGTGCTGAAGGACGTCATGGACTTCATTAAGGATCAGTCGC
TCTCGCACAGGAGTGTTGTGAAGGTTCTTTCCCTGAGGAAAGCCCAGGCCCAGAGCATCCTGGAAGTCCTGAAGATAACTCAGCACTGTG
CAGAATCCCTTGGGCAGCCCCACTGCTTCCATCCACCTTTCATACTTTTCCTGTTGGAACTTCTGACCTGTCAGAAAGATTTTACCAATT
ATTTTGGACACCTGGAAGGCTGTGGTGCTGATCTACACAAAGAAATTCGAGACACTTACTATCAACTTGTTCTGTTTTTGGTCAAAGCAG
TTAAAGGATTTAGTAGCCTAAATGACAGGTCCTTGCTCCCTGCCTTATCCTGTGTTCAGACAGCCCTGCTTCATCTTTTGGATATGGGCT
GGGAACCCAATGATCTCGCCTTCTTTGTTGATATTCAGTTACCAGATCTCCTCATGAAAATGTCACAGGAGAATATAAGTGTCCATGACA
GTGTGATCAGCCAATGGAGTGAAGAAGATGAGCTTGCTGATGCCAAGCAGAATTCAGAATGGATGGATGAGTGTCAGGATGGCATGTTTG
AGGCCTGGTATGAAAAAATAGCCCAGGAAGATCCAGAGAAGCAGAGGAAAATGCACATGTTCATTGCTCGCTACTGTGACCTGTTAAATG
TGGACATCTCTTGTGATGGGTGTGATGAGATTGCCCCCTGGCATCGATACCGCTGTCTGCAGTGCAGCGACATGGATCTCTGCAAAACTT
GCTTCCTAGGTGGGGTGAAGCCTGAGGGCCACGGAGACGACCATGAAATGGTCAACATGGAGTTTACCTGTGACCACTGCCAGGGTTTGA
TCATAGGCCGGAGGATGAACTGCAATGTTTGCGATGACTTTGATCTTTGCTACGGATGCTATGCAGCGAAGAAATACTCCTACGGCCATT
TGCCTACCCACAGCATCACGGCCCACCCAATGGTAACCATTCGGATCAGTGACCGGCAGAGGCTCATCCAGCCATATATCCATAACTACT
CCTGGCTGCTCTTTGCTGCCCTGGCTCTCTATAGCGCCCACCTGGCCAGTGCAGAGGATGTGGATGGGGAGAAGCTGGACCCCCAGACGC
GCAGCAGTGCCACCACCCTGCGGAGCCAGTGCATGCAGCTCGTCGGGGACTGTCTGATGAAGGCTCATCAGGGAAAAGGCCTTAAAGCTC
TAGCTTTGCTGGGTGTATTGCCAGATGGGGACTCGAGCCTAGAAGATCAGGCCCTACCAGTCACTGTGCCCACCGGAGCGTCAGAGGAGC
AGCTAGAGAAGAAAGCTGTCCAGGGTGCTGAGCTGTCAGAAGCAGGCAATGGAAAGAGAGCTGTTCATGAGGAAATCAGACCTGTAGATT
TCAAGCAGAGAAATAAGGCAGATAAAGGTGTATCATTATCGAAGGATCCTTCATGCCAGACCCAAATTTCAGATTCACCTGCAGATGCTA
GCCCACCTACAGGACTTCCAGATGCTGAAGATTCAGAAGTGTCATCTCAGAAGCCCATAGAGGAAAAAGCAGTTACTCCAAGCCCTGAGC
AAGTGTTTGCTGAGTGTTCCCAGAAGAGGATTTTGGGATTACTAGCAGCCATGTTACCTCCCTTAAAGTCGGGCCCCACGGTTCCCCTGA
TAGACCTGGAGCACGTCCTTCCACTCATGTTTCAGGTTGTCATCTCAAACGCAGGCCACCTGAATGAAACCTACCATCTCACCCTGGGTC
TTCTCGGCCAGTTAATTATCCGTCTTTTGCCAGCAGAGGTAGACGCCGCAGTGATCAAAGTCCTCTCAGCCAAACACAACCTGTTTGCTG
CAGGGGACAGTTCCATTGTGCCAGATGGCTGGAAAACCACCCACCTGCTCTTTAGCCTGGGAGCTGTGTGTCTGGACAGCCGGGTGGGCT
TGGACTGGGCGTGCTCCATGGCAGAGATCCTGCGGTCACTCAACAGTGCCCCACTGTGGCGTGATGTCATTGCCACCTTCACAGACCACT
GCATCAAGCAGCTGCCATTCCAGCTGAAGCACACCAACATCTTCACCCTGCTCGTGCTGGTTGGCTTCCCCCAGGTCCTCTGTGTGGGAA
CCCGCTGCGTTTATATGGATAATGCCAATGAACCCCATAATGTGATCATCTTGAAGCACTTTACTGAGAAGAACAGGGCTGTGATTGTTG
ATGTCAAAACTCGGAAGAGGAAAACAGTGAAGGACTACCAGCTGGTCCAGAAGGGAGGAGGACAAGAGTGTGGTGACTCTCGGGCCCAGC
TGAGCCAGTACTCCCAGCACTTTGCCTTTATCGCCAGTCACCTTCTGCAAAGCAGCATGGACAGCCATTGTCCCGAGGCAGTAGAAGCAA
CTTGGGTCCTGTCCCTGGCCCTGAAAGGATTGTATAAAACACTAAAGGCTCACGGTTTTGAGGAGATCCGTGCTACTTTCCTTCAGACCG
ATTTGCTGAAGTTGCTGGTGAAAAAGTGCAGCAAAGGGACTGGCTTTAGTAAAACGTGGCTCCTCCGGGACCTGGAAATTTTGTCCATCA
TGCTGTACTCCTCAAAAAAGGAGATCAACGCTTTGGCTGAGCACGGAGACCTAGAGCTGGATGAGCGAGGGGACCGAGAGGAAGAGGTGG
AACGGCCAGTCAGCAGCCCTGGCGACCCAGAGCAGAAAAAGCTGGACCCCCTTGAGGGCCTGGATGAGCCCACCAGAATATGTTTCTTGA
TGGCTCATGATGCCCTCAATGCCCCTCTGCACATTCTCCGGGCCATATACGAACTGCAGATGAAAAAGACCGATTATTTCTTCCTGGAGG
TTCAGAAGAGGTTTGATGGTGATGAGCTCACCACAGATGAAAGGATACGGTCCCTGGCTCAGCGGTGGCAGCCCAGTAAGAGTCTGAGGC
TGGAAGAACAGAGCGCCAAAGCTGTGGATACAGACATGATTATCCTGCCATGCTTGTCCCGGCCTGCACGCTGTGACCAAGCCACTGCTG
AATCGAACCCTGTGACCCAGAAGCTGATCTCCAGCACAGAGAGCGAACTGCAGCAGAGCTATGCCAAGCAGCGCCGTAGCAAGAGCGCCG
CCCTCCTGCACAAGGAGCTGAACTGCAAGAGTAAGAGGGCTGTCCGGGACTACCTCTTCCGAGTGAACGAGGCCACAGCTGTCCTGTACG
CCCGCCACGTGCTTGCATCCCTGCTCGCCGAGTGGCCTAGCCACGTGCCAGTGAGCGAGGACATCCTGGAGCTCAGTGGCCCTGCCCATA
TGACCTACATTTTGGATATGTTCATGCAGCTGGAAGAAAAGCATGAGTGGGAGAAGATCCTGCAGAAAGTGCTCCAGGGCTGCCGAGAGG
ACATGCTGGGGACCATGGCCCTGGCTGCATGCCAGTTCATGGAGGAGCCAGGAATGGAGGTGCAAGTGAGGGAGTCGAAACACCCGTATA
ACAACAACACCAACTTCGAGGATAAAGTTCACATTCCTGGTGCCATCTACCTCTCAATCAAATTCGACTCTCAGTGCAACACAGAGGAGG
GCTGTGACGAGTTAGCCATGTCCAGCAGCAGTGACTTCCAGCAAGACCGACACAGCTTCAGCGGGTCTCAGCAGAAGTGGAAAGATTTTG
AACTTCCAGGAGACACTCTGTATTACCGCTTCACCTCCGACATGAGCAACACCGAGTGGGGCTACAGATTCACCGTGACGGCCGGACACC
TGGGGCGGTTCCAGACAGGATTCGAGATTTTGAAGCAGATGTTGTCAGAAGAAAGGGTCGTGCCTCATCTCCCATTGGCAAAAATTTGGG
AATGGCTGGTGGGCGTGGCCTGTCGCCAGACTGGCCATCAACGATTAAAAGCCATCCACTTACTTCTGAGGATTGTGCGATGCTGCGGCC
ACAGTGACCTGTGTGACCTTGCGCTGTTGAAGCCCCTGTGGCAGCTCTTTACCCACATGGAGTACGGCCTGTTTGAGGACGTGACGCAGC
CCGGCATCCTCCTTCCCCTGCATCGTGCCCTCACTGAGCTCTTCTTCGTCACCGAGAACCGTGCCCAGGAGCTTGGCGTGCTGCAGGATT
ACCTGCTGGCCCTAACCACGGACGACCACCTTCTCCGCTGTGCGGCACAGGCTCTGCAGAACATTGCTGCCATCAGCCTGGCCATCAACT
ACCCAAACAAGGCCACCCGCCTCTGGAATGTGGAGTGTTAGCCCTTGGTGGGGCGTGCATGGGACTAGTTCATCTGCCACAGGGATTTTA
GAGCAGACATCTAACCTCATTCAGGAAAACTCCTGTAGCGCCAGTGCCCAGCTCTCCTTGAGCTGACCACTCCAGTTAGGATGCCAAGCA
GCCACGTCTCCAAGAGCTCCCGTGCGTAGGCTGGACACAAGCACAGGCTGTAGCATGGTGAAAATAAGCCAAGCAGTGCAGAATGCCTCA
GAAAGGGTGGGCAGGGGGCCCTTAAGAAGGTTCAGAGACCAGCCTTCTCCAGAGGCTGTCACTGCAGGAGCCGTGGGCCTGGGAAGACTT
GGAAGCGGCCTCTCTCAACTGGTTTCTGTCTCCGTGGAGCTGGAACTGCCTGCACTTGCCTTCAGAGGGAGGCACAGTCCACCCAGATCC
ACCTTTCCAGCAAGACCCCCAGTGGCTGCCCAGCCTGGGAGCACCTCTTTGCTTTTCACACCAAACCAAAACTGGCGAGAGCCCCTCCTA
GCCACCAGTGATCCCCAAGCATCCAGTACAGAACCAGGCATCGAGCTAGCTCCCTGCACGGCCGCACCCTCCCAGAGAACTCCTTGAGGA
GAACAAGTGCCCTTGGGGACAGCCGGCAGGCGCCCCTGTACGTCTGCTCATGCACCAGGCAGCACAGCCGCAGTTCCTCAGTTGTTGTTT
TGACATATTTCAGTTTCCACCTCACGTTTTTAGAGCAGAACCACACTGTCTCCCTGGAGGGGCTCGAGGGCATGACCGGGGACTGACCAT
TCTGTGAAAGGAGCAGAATGTGAGGAGCACGCGTGAGCTTATGTACCGTGAAGATGATCAGAGGATATCTTATTTTAAGAGTAAAAACCC
ACATAATTTTATTTCTGCTTGATAGTCATGGTAGTCTGTCATACCCACCTCTGGGACTCTGCGTGGCTGTTTGGCTGTCACTTGTAGCAA
TAACGACATTAGTTCTAGTCAGTGCTGTTTTACATTTTTCTTTTGATGGGTTTAGTCTTGCCCTGGAGTGCCGATGATGATTCTCCCTCC
AGAGCCACGCTTGGGAACATGAAGCAAGTCTGGCGTGTGGGCTGCGTGCCGGCCTTAGTGGGACCCGTGGGGTTGGAGCATGCCTTTAGG
GGCAGTGTCTGGGCCGAAGCACGTCCCACCACACAGTGCCAGAGCCAGAGAAGGGGCCCCACCACCAAGGCCAAGCTTGACCAGGTCAGC
ATTGCCATGGCCCAGTGTGCCCCGTGGCCTCTGAAGATCCCTCTGTGCAGGGTCTGCAGGGATCTGGATTGCAAGGGCCCAAGTCTGCAG
GTCTGGAAGCATCTTCCTATAAGAGCACTTTCGCCTTCTGGGTCAGGACTCCAAGGTGCAGCGGGCTTCACAGCCCTACAATTGGGTTCT
CAGCTAAGCCCCAGAGTTCTGGTAGAACCATCCCGGGGCGGGTGGAGGGTGGGATTTAAGGGAGACGGGAACACATGGGGCAGGTCCTGG
AACTTGGTGGCCTGAGGACTGAGGCCATTGCCCTGGTGGAAAGGCCTGGCCTGGTTCCTGTGGCTTGGGACCTGAATAGGCAGGTGCTGC
TGGCTCCGTAGAAACCCTTTTCCCATCTTTTGCTCTTTGCCAAACCTACCTTGCTTTGGGAGCTGCCTGCACCACCCCAGAGAAGGCCCC
ACCTTCTTCATCCCTCAGACCCGAGGAGGCCTCCCAGTAAGGAGTTTCCCAAGAGGGGACTCACAGGAAACAAGTCTTAGTGCTTGGGAG
GGAGGCCCCGCTGCGTGCTCAGACTCACAGCCAACCTGGAAGGTAGACGAGATAGCGCCACCCACGCCCCTCCACACCCCAGACTCCGAG
TAAAGCGGGCGGTAGGGCCGGAGTCACCTCCCCTATGGCAGTGGCCGCCGCTGTACTCCATCCTCCCGTCAGGAAGATCAGCTGTAAATA
AACGCTGGGCTCCCCAGAGCACCTGTCCGCCCACTGCCCTTGCTGTTCTGGGATCTTCGCTGCAGTTCACGGGAAACAAGCCTGAGTCCG
CTCGCACCCGCGGCTGCTCTCCCGGCTCGGCCCGGCCGCCTCTGTCTCCGGCCACCGGGTGGCGCTGCCGAGCCAGAGCCGCCGCGTCCC
GGCGCTTTCCAGGAGCCCCAGGCCCGGAGGAGGCGAAGCCCGCAGAGCAAAGGTGGAAACACGTGCCTACGCTGTAAAGAAATCCTGTTC
CAGAGCATACCTGTTGTACAAACAGACACTGTTCCTAACGAGAGGAGTGACGTATTTTCATCACCGTTTTTAATTTGTTTTCTTACGGGT
TTACGATTTTGAATTTTTCTTATTTGGTTGAAAGAATTTTGATTCTATCAGCCTGAGTGAGTTCAGCCTGTAAAAAGGATGTTAAGCTGT

>In-frame_ENST00000291074_ENST00000381638_TCGA-55-8087-01A_VPS53_chr17_600658_-_ZZEF1_chr17_4027345_length(amino acids)=2938AA_start in transcript=134_stop in transcript=8950
MMEEEELEFVEELEAVLQLTPEVQLAIEQVFPSQDPLDRADFNAVEYINTLFPTEQSLANIDEVVNKIRLKIRRLDDNIRTVVRGQTNVG
QDGRQAFAQFDAEGDGTVDAENMLEALKNSSGANLQGELSHIIRQLQACSLVPGFTDIFSESKEGLDIHSSMILRFLHRNRLSSAVMPYP
MLEHCNNMCTMRSSVLKESLDQLVQKEKESPGDLTRSPEMDKLKSVAKCYAYIETSSNSADIDKMTNGETSSYWQSDGSACSHWIRLKMK
PDVVLRHLSIAVAATDQSYMPQQVTVAVGRNASDLQEVRDVHIPSNVTGYVTLLENANVSQLYVQINIKRCLSDGCDTRIHGLRAVGFQR
VKKSGVSVSDASAIWYWSLLTSLVTASMETNPAFVQTVLHNTQKALRHMPPLSLSPGSTDFSTFLSPNVLEEVDSFLIRITSCCSTPEVE
LTLLAFALARGSVAKVMSSLCTITDHLDTQYDASSLILSMASVRQNLLLKYGKPLQLTLQACDVKGKEDKSGPENLLVEPWTRDGFLTET
GKTRASTIFSTGTESAFQVTQIRIMVRRGGIGAQCGLVFAYNSSSDKFCAEEHFKRFEKYDKWKLQELRQFVKSRIGCSSDDLGEDDPIG
WFELEEEWDEADVKLQQCRVAKYLMVKFLCTRQESAERLGVQGLTISGYLRPARAEAEQSVTCAHCRKDTEESVCGATLLLRTLQFIQQL
AHDLVQQKESGLKYKSFLDFAGLDLQIFWNFYSKLKQNPREECVSAQTLLLQLLQSCFSVLQGDVLAASEEEKAPIQSPKGVEAAKELYT
HLCDVVDKVDGDSVPMEILKQEVRNTLLNGAAIFFPNRQTRRNHLFTMMNVTEQEHKQSLQLTFRSLCTYFSDKDPGGLLLLPEKNDLAK
MNISEVLAVMDTLVSVAARECELLMLSGAPGEVGSVLFSLFWSVQGSLLSWCYLQLKSTDSGAKDLAVDLIEKYVGQFLASMRAILESLF
SQYSGKTIVERLCNSVFSMAARQLVIFLLDFCTLDIPHCVLLREFSVLTELLKKLCSGPEGGLRKLDVETWQQEQPVVLHTWTKESAHNY
ENNCHEVSVFVSPGATYFEVEFDDRCETEKRYDYLEFTDARGRKTRYDTKVGTDKWPKKVTFKAGPRLQFLFHSDSSHNEWGYKFTVTAC
GLPDVAVSWGLDLQLLVSRLMGRLASQCMALKSVRQLGSNMVVPQAKMALVLSSPLWKPVFRHQVCPELELEASWPTHPHRNSKEVKNIP
DDPCRHFLLDFAQSEPAQNFCGPYSELFKGFIQACRKQAPKTDIVAGSTIDQAVNATFAALVYRTPDLYEKLQKYVNSGGKIALSEEFAQ
VYSLADGIRIWMLEMKQKSLMSLGNEAEEKHSSEATEVNPESLAKECIEKSLLLLKFLPTGISSKESCEKLETADETSHLQPLNKRQRTS
SVVEEHFQASVSPTEAAPPATGDQSPGLGTQPKLPSSSGLPAADVSPATAEEPLSPSTPTRRPPFTRGRLRLLSFRSMEEARLVPTVKEK
YPVLKDVMDFIKDQSLSHRSVVKVLSLRKAQAQSILEVLKITQHCAESLGQPHCFHPPFILFLLELLTCQKDFTNYFGHLEGCGADLHKE
IRDTYYQLVLFLVKAVKGFSSLNDRSLLPALSCVQTALLHLLDMGWEPNDLAFFVDIQLPDLLMKMSQENISVHDSVISQWSEEDELADA
KQNSEWMDECQDGMFEAWYEKIAQEDPEKQRKMHMFIARYCDLLNVDISCDGCDEIAPWHRYRCLQCSDMDLCKTCFLGGVKPEGHGDDH
EMVNMEFTCDHCQGLIIGRRMNCNVCDDFDLCYGCYAAKKYSYGHLPTHSITAHPMVTIRISDRQRLIQPYIHNYSWLLFAALALYSAHL
ASAEDVDGEKLDPQTRSSATTLRSQCMQLVGDCLMKAHQGKGLKALALLGVLPDGDSSLEDQALPVTVPTGASEEQLEKKAVQGAELSEA
GNGKRAVHEEIRPVDFKQRNKADKGVSLSKDPSCQTQISDSPADASPPTGLPDAEDSEVSSQKPIEEKAVTPSPEQVFAECSQKRILGLL
AAMLPPLKSGPTVPLIDLEHVLPLMFQVVISNAGHLNETYHLTLGLLGQLIIRLLPAEVDAAVIKVLSAKHNLFAAGDSSIVPDGWKTTH
LLFSLGAVCLDSRVGLDWACSMAEILRSLNSAPLWRDVIATFTDHCIKQLPFQLKHTNIFTLLVLVGFPQVLCVGTRCVYMDNANEPHNV
IILKHFTEKNRAVIVDVKTRKRKTVKDYQLVQKGGGQECGDSRAQLSQYSQHFAFIASHLLQSSMDSHCPEAVEATWVLSLALKGLYKTL
KAHGFEEIRATFLQTDLLKLLVKKCSKGTGFSKTWLLRDLEILSIMLYSSKKEINALAEHGDLELDERGDREEEVERPVSSPGDPEQKKL
DPLEGLDEPTRICFLMAHDALNAPLHILRAIYELQMKKTDYFFLEVQKRFDGDELTTDERIRSLAQRWQPSKSLRLEEQSAKAVDTDMII
LPCLSRPARCDQATAESNPVTQKLISSTESELQQSYAKQRRSKSAALLHKELNCKSKRAVRDYLFRVNEATAVLYARHVLASLLAEWPSH
VPVSEDILELSGPAHMTYILDMFMQLEEKHEWEKILQKVLQGCREDMLGTMALAACQFMEEPGMEVQVRESKHPYNNNTNFEDKVHIPGA
IYLSIKFDSQCNTEEGCDELAMSSSSDFQQDRHSFSGSQQKWKDFELPGDTLYYRFTSDMSNTEWGYRFTVTAGHLGRFQTGFEILKQML
SEERVVPHLPLAKIWEWLVGVACRQTGHQRLKAIHLLLRIVRCCGHSDLCDLALLKPLWQLFTHMEYGLFEDVTQPGILLPLHRALTELF

--------------------------------------------------------------
>In-frame_ENST00000571805_ENST00000381638_TCGA-55-8087-01A_VPS53_chr17_600658_-_ZZEF1_chr17_4027345_length(transcript)=11399nt_BP=422nt
AGCGGCTGACAGAGCGGCTGACGGAGCCGGGCTCACCAGGTCGCTGCCGCGAGGGAGTTGCTGTGCTGGGGCCTGGGTGGCGGCTGGAGG
CCTGAGTTGGGCTCGCGGCGGGGGTCGGCAGGGGGCCGGGTGGCGGAATGATGGAGGAGGAGGAACTGGAGTTCGTGGAGGAGCTGGAAG
CCGTGCTGCAGCTCACGCCCGAGGTGCAGCTGGCCATCGAGCAGGTGTTTCCAAGCCAGGACCCTCTAGATCGAGCAGATTTCAATGCTG
TTGAGTATATCAATACCCTGTTCCCAACCGAGCAATCTCTGGCGAACATAGACGAAGTCGTGAACAAAATTAGGCTGAAAATAAGGAGAC
TGGATGACAATATTCGAACTGTTGTAAGAGGTCAGACGAACGTGGGGCAGGATGGACGGCAAGCCTTTGCCCAGTTTGATGCTGAGGGTG
ATGGGACAGTTGATGCCGAGAACATGTTGGAGGCCCTCAAGAATTCCAGTGGAGCTAATCTTCAGGGGGAGCTGAGCCACATCATCAGAC
AACTACAGGCCTGCTCTCTGGTTCCAGGTTTCACAGACATATTTTCAGAGTCGAAGGAGGGCCTTGATATTCACTCGTCAATGATACTGC
GCTTCCTGCACCGCAATCGGCTCTCCAGCGCGGTGATGCCCTACCCGATGCTGGAGCACTGCAATAACATGTGCACCATGCGGTCTTCCG
TCCTGAAGGAGTCTCTGGATCAGCTGGTACAAAAGGAAAAGGAAAGCCCTGGAGATCTAACTAGAAGTCCAGAGATGGATAAACTCAAGT
CAGTAGCAAAGTGCTATGCTTATATAGAAACATCCTCCAACTCGGCAGACATTGACAAGATGACAAATGGAGAAACCTCATCCTACTGGC
AGTCAGATGGCAGTGCCTGTTCACACTGGATTCGTTTAAAAATGAAGCCAGATGTTGTGCTTAGGCACCTGTCCATTGCAGTGGCTGCCA
CTGACCAGAGCTACATGCCACAGCAGGTGACAGTAGCTGTAGGGAGGAATGCCAGCGATCTTCAGGAAGTCCGAGATGTGCACATCCCCA
GCAATGTCACTGGCTATGTGACGCTGCTGGAAAATGCCAACGTCAGTCAGCTCTATGTCCAGATTAACATAAAGCGTTGTCTTAGCGATG
GCTGCGACACTAGAATTCATGGTCTCAGGGCTGTTGGCTTTCAGAGAGTTAAGAAGTCTGGGGTCTCAGTCTCAGATGCTTCTGCAATAT
GGTATTGGTCTCTGCTGACATCTCTGGTGACGGCTTCTATGGAGACAAATCCCGCCTTTGTCCAGACAGTGCTGCACAATACTCAGAAGG
CGCTGCGGCACATGCCTCCACTCTCTCTCTCACCAGGATCTACAGATTTCTCAACTTTCCTCTCCCCTAATGTGCTGGAAGAAGTGGACA
GTTTCCTCATAAGGATAACTAGCTGCTGTTCTACCCCAGAGGTAGAACTGACTCTTCTGGCTTTTGCTCTCGCAAGAGGAAGTGTTGCCA
AAGTCATGAGCTCTCTATGCACCATCACTGACCATCTGGACACGCAGTATGATGCCTCATCCCTCATCTTGTCCATGGCGTCAGTCAGAC
AGAACCTGCTCCTCAAATATGGTAAACCTCTCCAGTTGACTCTTCAGGCATGTGATGTCAAAGGAAAAGAAGATAAGTCTGGACCCGAAA
ACCTCCTTGTTGAACCGTGGACAAGGGATGGTTTTCTTACGGAAACTGGAAAAACCAGAGCCAGCACTATTTTTTCTACCGGAACTGAAT
CTGCCTTCCAAGTTACACAGATAAGAATTATGGTTCGACGTGGTGGCATTGGTGCCCAGTGTGGGTTGGTGTTTGCCTATAACTCATCTT
CAGATAAATTTTGTGCGGAAGAACACTTCAAAAGGTTTGAAAAATATGACAAATGGAAGCTTCAGGAGCTCAGGCAATTTGTAAAAAGCA
GGATTGGTTGCTCATCTGATGACCTTGGAGAGGATGATCCTATTGGCTGGTTTGAACTGGAAGAAGAATGGGATGAAGCAGATGTGAAGC
TGCAACAGTGCAGAGTTGCCAAATATTTGATGGTGAAGTTCCTCTGCACCCGTCAGGAGTCAGCAGAGCGCTTGGGAGTGCAAGGCTTGA
CCATCAGTGGGTACCTCCGGCCTGCAAGAGCAGAAGCAGAACAGAGCGTCACCTGTGCACACTGCAGAAAGGACACAGAGGAGAGTGTCT
GTGGGGCCACGTTGCTCCTCAGGACCCTTCAGTTTATCCAGCAGCTCGCCCATGACCTGGTGCAGCAGAAGGAAAGTGGCTTAAAATATA
AATCTTTTCTGGACTTCGCGGGTCTTGATTTGCAGATCTTCTGGAATTTTTACAGTAAATTAAAGCAAAACCCGAGGGAAGAATGCGTCT
CTGCCCAAACCCTGCTTCTGCAGCTACTCCAGAGCTGCTTCTCTGTGCTGCAGGGAGATGTACTGGCTGCTTCTGAGGAGGAAAAGGCTC
CAATCCAAAGCCCTAAAGGAGTAGAGGCTGCCAAGGAGCTGTACACACACTTGTGTGATGTGGTGGACAAGGTGGATGGAGACTCTGTGC
CCATGGAGATACTAAAACAAGAAGTCAGGAATACCCTTCTCAATGGGGCTGCCATCTTCTTTCCTAATCGACAGACCCGACGGAACCATC
TCTTCACCATGATGAATGTCACCGAGCAGGAGCACAAGCAGTCCCTGCAGCTCACTTTCCGTTCACTGTGCACGTATTTTAGTGACAAGG
ATCCAGGCGGCCTTCTTCTTTTACCTGAGAAGAACGACCTGGCCAAGATGAACATCAGTGAAGTCCTGGCGGTCATGGACACTCTCGTCT
CTGTTGCTGCTCGAGAGTGCGAGCTGTTAATGCTCAGTGGGGCCCCAGGGGAGGTGGGCTCTGTGCTCTTCTCCCTGTTCTGGTCCGTCC
AAGGCAGCCTGCTATCCTGGTGCTACCTGCAGCTGAAGAGCACGGACTCTGGAGCCAAAGATCTTGCCGTGGACCTTATTGAAAAATATG
TGGGCCAGTTTCTGGCAAGCATGAGAGCGATTTTGGAATCCCTTTTCTCACAGTACAGTGGAAAAACCATAGTAGAAAGATTATGTAACT
CAGTGTTTTCAATGGCAGCTCGTCAACTGGTTATCTTCCTGCTGGACTTCTGCACTTTAGACATCCCACACTGCGTGCTCTTGAGAGAGT
TCAGCGTCCTCACAGAACTCCTGAAGAAGCTCTGTAGTGGCCCCGAAGGAGGACTGAGGAAGCTGGATGTTGAGACCTGGCAACAGGAAC
AGCCTGTGGTGTTACATACGTGGACGAAGGAATCTGCCCACAACTATGAAAATAATTGCCATGAGGTATCCGTCTTTGTTAGCCCAGGGG
CAACCTATTTTGAAGTGGAATTCGATGACAGGTGTGAAACTGAAAAAAGGTATGATTATCTGGAATTTACCGACGCTAGAGGTCGGAAAA
CACGCTATGACACAAAAGTTGGCACTGATAAATGGCCCAAGAAAGTGACCTTCAAGGCCGGTCCTCGGTTGCAGTTCCTCTTTCACTCTG
ACAGCAGTCACAACGAATGGGGCTACAAATTCACTGTCACTGCCTGTGGGCTGCCCGATGTTGCCGTGTCTTGGGGGCTGGATTTACAGC
TCCTCGTCTCCCGGCTGATGGGACGCCTGGCTTCCCAGTGCATGGCGCTCAAGTCTGTGCGCCAGTTAGGAAGTAACATGGTAGTACCTC
AGGCAAAAATGGCATTAGTCCTAAGCTCCCCACTGTGGAAACCTGTCTTCAGGCATCAGGTTTGTCCAGAGTTGGAATTAGAAGCAAGCT
GGCCCACTCACCCACACCGGAATAGTAAGGAGGTTAAGAACATTCCTGACGACCCCTGCCGCCATTTTCTTCTTGATTTTGCCCAGTCAG
AGCCTGCTCAGAACTTCTGTGGGCCATATTCAGAACTTTTCAAAGGATTCATACAGGCATGTAGAAAACAGGCCCCAAAGACAGATATAG
TTGCTGGTTCCACTATTGATCAAGCTGTGAACGCCACCTTTGCTGCTCTGGTGTATCGCACTCCAGATTTATATGAGAAGCTGCAAAAAT
ATGTCAACAGTGGGGGCAAAATAGCCCTGAGTGAAGAGTTTGCCCAGGTTTATTCCTTGGCAGATGGGATTCGAATATGGATGTTAGAGA
TGAAGCAGAAGTCCCTGATGAGCCTGGGGAATGAAGCAGAAGAAAAACATAGTTCAGAAGCTACTGAGGTGAACCCTGAGAGCCTGGCAA
AAGAGTGCATTGAGAAGAGTCTTCTATTACTAAAATTTCTGCCCACGGGCATAAGTTCAAAAGAAAGCTGCGAAAAGTTGGAGACTGCTG
ATGAAACCAGTCATCTCCAGCCACTCAACAAGCGTCAGAGGACAAGCTCTGTGGTGGAAGAGCATTTCCAAGCCTCAGTATCTCCCACTG
AAGCCGCACCCCCTGCCACAGGAGACCAGAGTCCTGGCCTGGGCACCCAGCCAAAGCTGCCATCCAGCAGTGGCCTTCCTGCTGCAGACG
TGTCCCCTGCCACAGCTGAAGAGCCCTTGTCACCTTCCACACCCACCCGCCGGCCTCCCTTCACCCGAGGGCGACTCCGGCTGCTCTCCT
TTCGATCCATGGAGGAGGCCAGACTGGTGCCCACAGTGAAAGAGAAATACCCTGTGCTGAAGGACGTCATGGACTTCATTAAGGATCAGT
CGCTCTCGCACAGGAGTGTTGTGAAGGTTCTTTCCCTGAGGAAAGCCCAGGCCCAGAGCATCCTGGAAGTCCTGAAGATAACTCAGCACT
GTGCAGAATCCCTTGGGCAGCCCCACTGCTTCCATCCACCTTTCATACTTTTCCTGTTGGAACTTCTGACCTGTCAGAAAGATTTTACCA
ATTATTTTGGACACCTGGAAGGCTGTGGTGCTGATCTACACAAAGAAATTCGAGACACTTACTATCAACTTGTTCTGTTTTTGGTCAAAG
CAGTTAAAGGATTTAGTAGCCTAAATGACAGGTCCTTGCTCCCTGCCTTATCCTGTGTTCAGACAGCCCTGCTTCATCTTTTGGATATGG
GCTGGGAACCCAATGATCTCGCCTTCTTTGTTGATATTCAGTTACCAGATCTCCTCATGAAAATGTCACAGGAGAATATAAGTGTCCATG
ACAGTGTGATCAGCCAATGGAGTGAAGAAGATGAGCTTGCTGATGCCAAGCAGAATTCAGAATGGATGGATGAGTGTCAGGATGGCATGT
TTGAGGCCTGGTATGAAAAAATAGCCCAGGAAGATCCAGAGAAGCAGAGGAAAATGCACATGTTCATTGCTCGCTACTGTGACCTGTTAA
ATGTGGACATCTCTTGTGATGGGTGTGATGAGATTGCCCCCTGGCATCGATACCGCTGTCTGCAGTGCAGCGACATGGATCTCTGCAAAA
CTTGCTTCCTAGGTGGGGTGAAGCCTGAGGGCCACGGAGACGACCATGAAATGGTCAACATGGAGTTTACCTGTGACCACTGCCAGGGTT
TGATCATAGGCCGGAGGATGAACTGCAATGTTTGCGATGACTTTGATCTTTGCTACGGATGCTATGCAGCGAAGAAATACTCCTACGGCC
ATTTGCCTACCCACAGCATCACGGCCCACCCAATGGTAACCATTCGGATCAGTGACCGGCAGAGGCTCATCCAGCCATATATCCATAACT
ACTCCTGGCTGCTCTTTGCTGCCCTGGCTCTCTATAGCGCCCACCTGGCCAGTGCAGAGGATGTGGATGGGGAGAAGCTGGACCCCCAGA
CGCGCAGCAGTGCCACCACCCTGCGGAGCCAGTGCATGCAGCTCGTCGGGGACTGTCTGATGAAGGCTCATCAGGGAAAAGGCCTTAAAG
CTCTAGCTTTGCTGGGTGTATTGCCAGATGGGGACTCGAGCCTAGAAGATCAGGCCCTACCAGTCACTGTGCCCACCGGAGCGTCAGAGG
AGCAGCTAGAGAAGAAAGCTGTCCAGGGTGCTGAGCTGTCAGAAGCAGGCAATGGAAAGAGAGCTGTTCATGAGGAAATCAGACCTGTAG
ATTTCAAGCAGAGAAATAAGGCAGATAAAGGTGTATCATTATCGAAGGATCCTTCATGCCAGACCCAAATTTCAGATTCACCTGCAGATG
CTAGCCCACCTACAGGACTTCCAGATGCTGAAGATTCAGAAGTGTCATCTCAGAAGCCCATAGAGGAAAAAGCAGTTACTCCAAGCCCTG
AGCAAGTGTTTGCTGAGTGTTCCCAGAAGAGGATTTTGGGATTACTAGCAGCCATGTTACCTCCCTTAAAGTCGGGCCCCACGGTTCCCC
TGATAGACCTGGAGCACGTCCTTCCACTCATGTTTCAGGTTGTCATCTCAAACGCAGGCCACCTGAATGAAACCTACCATCTCACCCTGG
GTCTTCTCGGCCAGTTAATTATCCGTCTTTTGCCAGCAGAGGTAGACGCCGCAGTGATCAAAGTCCTCTCAGCCAAACACAACCTGTTTG
CTGCAGGGGACAGTTCCATTGTGCCAGATGGCTGGAAAACCACCCACCTGCTCTTTAGCCTGGGAGCTGTGTGTCTGGACAGCCGGGTGG
GCTTGGACTGGGCGTGCTCCATGGCAGAGATCCTGCGGTCACTCAACAGTGCCCCACTGTGGCGTGATGTCATTGCCACCTTCACAGACC
ACTGCATCAAGCAGCTGCCATTCCAGCTGAAGCACACCAACATCTTCACCCTGCTCGTGCTGGTTGGCTTCCCCCAGGTCCTCTGTGTGG
GAACCCGCTGCGTTTATATGGATAATGCCAATGAACCCCATAATGTGATCATCTTGAAGCACTTTACTGAGAAGAACAGGGCTGTGATTG
TTGATGTCAAAACTCGGAAGAGGAAAACAGTGAAGGACTACCAGCTGGTCCAGAAGGGAGGAGGACAAGAGTGTGGTGACTCTCGGGCCC
AGCTGAGCCAGTACTCCCAGCACTTTGCCTTTATCGCCAGTCACCTTCTGCAAAGCAGCATGGACAGCCATTGTCCCGAGGCAGTAGAAG
CAACTTGGGTCCTGTCCCTGGCCCTGAAAGGATTGTATAAAACACTAAAGGCTCACGGTTTTGAGGAGATCCGTGCTACTTTCCTTCAGA
CCGATTTGCTGAAGTTGCTGGTGAAAAAGTGCAGCAAAGGGACTGGCTTTAGTAAAACGTGGCTCCTCCGGGACCTGGAAATTTTGTCCA
TCATGCTGTACTCCTCAAAAAAGGAGATCAACGCTTTGGCTGAGCACGGAGACCTAGAGCTGGATGAGCGAGGGGACCGAGAGGAAGAGG
TGGAACGGCCAGTCAGCAGCCCTGGCGACCCAGAGCAGAAAAAGCTGGACCCCCTTGAGGGCCTGGATGAGCCCACCAGAATATGTTTCT
TGATGGCTCATGATGCCCTCAATGCCCCTCTGCACATTCTCCGGGCCATATACGAACTGCAGATGAAAAAGACCGATTATTTCTTCCTGG
AGGTTCAGAAGAGGTTTGATGGTGATGAGCTCACCACAGATGAAAGGATACGGTCCCTGGCTCAGCGGTGGCAGCCCAGTAAGAGTCTGA
GGCTGGAAGAACAGAGCGCCAAAGCTGTGGATACAGACATGATTATCCTGCCATGCTTGTCCCGGCCTGCACGCTGTGACCAAGCCACTG
CTGAATCGAACCCTGTGACCCAGAAGCTGATCTCCAGCACAGAGAGCGAACTGCAGCAGAGCTATGCCAAGCAGCGCCGTAGCAAGAGCG
CCGCCCTCCTGCACAAGGAGCTGAACTGCAAGAGTAAGAGGGCTGTCCGGGACTACCTCTTCCGAGTGAACGAGGCCACAGCTGTCCTGT
ACGCCCGCCACGTGCTTGCATCCCTGCTCGCCGAGTGGCCTAGCCACGTGCCAGTGAGCGAGGACATCCTGGAGCTCAGTGGCCCTGCCC
ATATGACCTACATTTTGGATATGTTCATGCAGCTGGAAGAAAAGCATGAGTGGGAGAAGATCCTGCAGAAAGTGCTCCAGGGCTGCCGAG
AGGACATGCTGGGGACCATGGCCCTGGCTGCATGCCAGTTCATGGAGGAGCCAGGAATGGAGGTGCAAGTGAGGGAGTCGAAACACCCGT
ATAACAACAACACCAACTTCGAGGATAAAGTTCACATTCCTGGTGCCATCTACCTCTCAATCAAATTCGACTCTCAGTGCAACACAGAGG
AGGGCTGTGACGAGTTAGCCATGTCCAGCAGCAGTGACTTCCAGCAAGACCGACACAGCTTCAGCGGGTCTCAGCAGAAGTGGAAAGATT
TTGAACTTCCAGGAGACACTCTGTATTACCGCTTCACCTCCGACATGAGCAACACCGAGTGGGGCTACAGATTCACCGTGACGGCCGGAC
ACCTGGGGCGGTTCCAGACAGGATTCGAGATTTTGAAGCAGATGTTGTCAGAAGAAAGGGTCGTGCCTCATCTCCCATTGGCAAAAATTT
GGGAATGGCTGGTGGGCGTGGCCTGTCGCCAGACTGGCCATCAACGATTAAAAGCCATCCACTTACTTCTGAGGATTGTGCGATGCTGCG
GCCACAGTGACCTGTGTGACCTTGCGCTGTTGAAGCCCCTGTGGCAGCTCTTTACCCACATGGAGTACGGCCTGTTTGAGGACGTGACGC
AGCCCGGCATCCTCCTTCCCCTGCATCGTGCCCTCACTGAGCTCTTCTTCGTCACCGAGAACCGTGCCCAGGAGCTTGGCGTGCTGCAGG
ATTACCTGCTGGCCCTAACCACGGACGACCACCTTCTCCGCTGTGCGGCACAGGCTCTGCAGAACATTGCTGCCATCAGCCTGGCCATCA
ACTACCCAAACAAGGCCACCCGCCTCTGGAATGTGGAGTGTTAGCCCTTGGTGGGGCGTGCATGGGACTAGTTCATCTGCCACAGGGATT
TTAGAGCAGACATCTAACCTCATTCAGGAAAACTCCTGTAGCGCCAGTGCCCAGCTCTCCTTGAGCTGACCACTCCAGTTAGGATGCCAA
GCAGCCACGTCTCCAAGAGCTCCCGTGCGTAGGCTGGACACAAGCACAGGCTGTAGCATGGTGAAAATAAGCCAAGCAGTGCAGAATGCC
TCAGAAAGGGTGGGCAGGGGGCCCTTAAGAAGGTTCAGAGACCAGCCTTCTCCAGAGGCTGTCACTGCAGGAGCCGTGGGCCTGGGAAGA
CTTGGAAGCGGCCTCTCTCAACTGGTTTCTGTCTCCGTGGAGCTGGAACTGCCTGCACTTGCCTTCAGAGGGAGGCACAGTCCACCCAGA
TCCACCTTTCCAGCAAGACCCCCAGTGGCTGCCCAGCCTGGGAGCACCTCTTTGCTTTTCACACCAAACCAAAACTGGCGAGAGCCCCTC
CTAGCCACCAGTGATCCCCAAGCATCCAGTACAGAACCAGGCATCGAGCTAGCTCCCTGCACGGCCGCACCCTCCCAGAGAACTCCTTGA
GGAGAACAAGTGCCCTTGGGGACAGCCGGCAGGCGCCCCTGTACGTCTGCTCATGCACCAGGCAGCACAGCCGCAGTTCCTCAGTTGTTG
TTTTGACATATTTCAGTTTCCACCTCACGTTTTTAGAGCAGAACCACACTGTCTCCCTGGAGGGGCTCGAGGGCATGACCGGGGACTGAC
CATTCTGTGAAAGGAGCAGAATGTGAGGAGCACGCGTGAGCTTATGTACCGTGAAGATGATCAGAGGATATCTTATTTTAAGAGTAAAAA
CCCACATAATTTTATTTCTGCTTGATAGTCATGGTAGTCTGTCATACCCACCTCTGGGACTCTGCGTGGCTGTTTGGCTGTCACTTGTAG
CAATAACGACATTAGTTCTAGTCAGTGCTGTTTTACATTTTTCTTTTGATGGGTTTAGTCTTGCCCTGGAGTGCCGATGATGATTCTCCC
TCCAGAGCCACGCTTGGGAACATGAAGCAAGTCTGGCGTGTGGGCTGCGTGCCGGCCTTAGTGGGACCCGTGGGGTTGGAGCATGCCTTT
AGGGGCAGTGTCTGGGCCGAAGCACGTCCCACCACACAGTGCCAGAGCCAGAGAAGGGGCCCCACCACCAAGGCCAAGCTTGACCAGGTC
AGCATTGCCATGGCCCAGTGTGCCCCGTGGCCTCTGAAGATCCCTCTGTGCAGGGTCTGCAGGGATCTGGATTGCAAGGGCCCAAGTCTG
CAGGTCTGGAAGCATCTTCCTATAAGAGCACTTTCGCCTTCTGGGTCAGGACTCCAAGGTGCAGCGGGCTTCACAGCCCTACAATTGGGT
TCTCAGCTAAGCCCCAGAGTTCTGGTAGAACCATCCCGGGGCGGGTGGAGGGTGGGATTTAAGGGAGACGGGAACACATGGGGCAGGTCC
TGGAACTTGGTGGCCTGAGGACTGAGGCCATTGCCCTGGTGGAAAGGCCTGGCCTGGTTCCTGTGGCTTGGGACCTGAATAGGCAGGTGC
TGCTGGCTCCGTAGAAACCCTTTTCCCATCTTTTGCTCTTTGCCAAACCTACCTTGCTTTGGGAGCTGCCTGCACCACCCCAGAGAAGGC
CCCACCTTCTTCATCCCTCAGACCCGAGGAGGCCTCCCAGTAAGGAGTTTCCCAAGAGGGGACTCACAGGAAACAAGTCTTAGTGCTTGG
GAGGGAGGCCCCGCTGCGTGCTCAGACTCACAGCCAACCTGGAAGGTAGACGAGATAGCGCCACCCACGCCCCTCCACACCCCAGACTCC
GAGTAAAGCGGGCGGTAGGGCCGGAGTCACCTCCCCTATGGCAGTGGCCGCCGCTGTACTCCATCCTCCCGTCAGGAAGATCAGCTGTAA
ATAAACGCTGGGCTCCCCAGAGCACCTGTCCGCCCACTGCCCTTGCTGTTCTGGGATCTTCGCTGCAGTTCACGGGAAACAAGCCTGAGT
CCGCTCGCACCCGCGGCTGCTCTCCCGGCTCGGCCCGGCCGCCTCTGTCTCCGGCCACCGGGTGGCGCTGCCGAGCCAGAGCCGCCGCGT
CCCGGCGCTTTCCAGGAGCCCCAGGCCCGGAGGAGGCGAAGCCCGCAGAGCAAAGGTGGAAACACGTGCCTACGCTGTAAAGAAATCCTG
TTCCAGAGCATACCTGTTGTACAAACAGACACTGTTCCTAACGAGAGGAGTGACGTATTTTCATCACCGTTTTTAATTTGTTTTCTTACG
GGTTTACGATTTTGAATTTTTCTTATTTGGTTGAAAGAATTTTGATTCTATCAGCCTGAGTGAGTTCAGCCTGTAAAAAGGATGTTAAGC

>In-frame_ENST00000571805_ENST00000381638_TCGA-55-8087-01A_VPS53_chr17_600658_-_ZZEF1_chr17_4027345_length(amino acids)=2938AA_start in transcript=137_stop in transcript=8953
MMEEEELEFVEELEAVLQLTPEVQLAIEQVFPSQDPLDRADFNAVEYINTLFPTEQSLANIDEVVNKIRLKIRRLDDNIRTVVRGQTNVG
QDGRQAFAQFDAEGDGTVDAENMLEALKNSSGANLQGELSHIIRQLQACSLVPGFTDIFSESKEGLDIHSSMILRFLHRNRLSSAVMPYP
MLEHCNNMCTMRSSVLKESLDQLVQKEKESPGDLTRSPEMDKLKSVAKCYAYIETSSNSADIDKMTNGETSSYWQSDGSACSHWIRLKMK
PDVVLRHLSIAVAATDQSYMPQQVTVAVGRNASDLQEVRDVHIPSNVTGYVTLLENANVSQLYVQINIKRCLSDGCDTRIHGLRAVGFQR
VKKSGVSVSDASAIWYWSLLTSLVTASMETNPAFVQTVLHNTQKALRHMPPLSLSPGSTDFSTFLSPNVLEEVDSFLIRITSCCSTPEVE
LTLLAFALARGSVAKVMSSLCTITDHLDTQYDASSLILSMASVRQNLLLKYGKPLQLTLQACDVKGKEDKSGPENLLVEPWTRDGFLTET
GKTRASTIFSTGTESAFQVTQIRIMVRRGGIGAQCGLVFAYNSSSDKFCAEEHFKRFEKYDKWKLQELRQFVKSRIGCSSDDLGEDDPIG
WFELEEEWDEADVKLQQCRVAKYLMVKFLCTRQESAERLGVQGLTISGYLRPARAEAEQSVTCAHCRKDTEESVCGATLLLRTLQFIQQL
AHDLVQQKESGLKYKSFLDFAGLDLQIFWNFYSKLKQNPREECVSAQTLLLQLLQSCFSVLQGDVLAASEEEKAPIQSPKGVEAAKELYT
HLCDVVDKVDGDSVPMEILKQEVRNTLLNGAAIFFPNRQTRRNHLFTMMNVTEQEHKQSLQLTFRSLCTYFSDKDPGGLLLLPEKNDLAK
MNISEVLAVMDTLVSVAARECELLMLSGAPGEVGSVLFSLFWSVQGSLLSWCYLQLKSTDSGAKDLAVDLIEKYVGQFLASMRAILESLF
SQYSGKTIVERLCNSVFSMAARQLVIFLLDFCTLDIPHCVLLREFSVLTELLKKLCSGPEGGLRKLDVETWQQEQPVVLHTWTKESAHNY
ENNCHEVSVFVSPGATYFEVEFDDRCETEKRYDYLEFTDARGRKTRYDTKVGTDKWPKKVTFKAGPRLQFLFHSDSSHNEWGYKFTVTAC
GLPDVAVSWGLDLQLLVSRLMGRLASQCMALKSVRQLGSNMVVPQAKMALVLSSPLWKPVFRHQVCPELELEASWPTHPHRNSKEVKNIP
DDPCRHFLLDFAQSEPAQNFCGPYSELFKGFIQACRKQAPKTDIVAGSTIDQAVNATFAALVYRTPDLYEKLQKYVNSGGKIALSEEFAQ
VYSLADGIRIWMLEMKQKSLMSLGNEAEEKHSSEATEVNPESLAKECIEKSLLLLKFLPTGISSKESCEKLETADETSHLQPLNKRQRTS
SVVEEHFQASVSPTEAAPPATGDQSPGLGTQPKLPSSSGLPAADVSPATAEEPLSPSTPTRRPPFTRGRLRLLSFRSMEEARLVPTVKEK
YPVLKDVMDFIKDQSLSHRSVVKVLSLRKAQAQSILEVLKITQHCAESLGQPHCFHPPFILFLLELLTCQKDFTNYFGHLEGCGADLHKE
IRDTYYQLVLFLVKAVKGFSSLNDRSLLPALSCVQTALLHLLDMGWEPNDLAFFVDIQLPDLLMKMSQENISVHDSVISQWSEEDELADA
KQNSEWMDECQDGMFEAWYEKIAQEDPEKQRKMHMFIARYCDLLNVDISCDGCDEIAPWHRYRCLQCSDMDLCKTCFLGGVKPEGHGDDH
EMVNMEFTCDHCQGLIIGRRMNCNVCDDFDLCYGCYAAKKYSYGHLPTHSITAHPMVTIRISDRQRLIQPYIHNYSWLLFAALALYSAHL
ASAEDVDGEKLDPQTRSSATTLRSQCMQLVGDCLMKAHQGKGLKALALLGVLPDGDSSLEDQALPVTVPTGASEEQLEKKAVQGAELSEA
GNGKRAVHEEIRPVDFKQRNKADKGVSLSKDPSCQTQISDSPADASPPTGLPDAEDSEVSSQKPIEEKAVTPSPEQVFAECSQKRILGLL
AAMLPPLKSGPTVPLIDLEHVLPLMFQVVISNAGHLNETYHLTLGLLGQLIIRLLPAEVDAAVIKVLSAKHNLFAAGDSSIVPDGWKTTH
LLFSLGAVCLDSRVGLDWACSMAEILRSLNSAPLWRDVIATFTDHCIKQLPFQLKHTNIFTLLVLVGFPQVLCVGTRCVYMDNANEPHNV
IILKHFTEKNRAVIVDVKTRKRKTVKDYQLVQKGGGQECGDSRAQLSQYSQHFAFIASHLLQSSMDSHCPEAVEATWVLSLALKGLYKTL
KAHGFEEIRATFLQTDLLKLLVKKCSKGTGFSKTWLLRDLEILSIMLYSSKKEINALAEHGDLELDERGDREEEVERPVSSPGDPEQKKL
DPLEGLDEPTRICFLMAHDALNAPLHILRAIYELQMKKTDYFFLEVQKRFDGDELTTDERIRSLAQRWQPSKSLRLEEQSAKAVDTDMII
LPCLSRPARCDQATAESNPVTQKLISSTESELQQSYAKQRRSKSAALLHKELNCKSKRAVRDYLFRVNEATAVLYARHVLASLLAEWPSH
VPVSEDILELSGPAHMTYILDMFMQLEEKHEWEKILQKVLQGCREDMLGTMALAACQFMEEPGMEVQVRESKHPYNNNTNFEDKVHIPGA
IYLSIKFDSQCNTEEGCDELAMSSSSDFQQDRHSFSGSQQKWKDFELPGDTLYYRFTSDMSNTEWGYRFTVTAGHLGRFQTGFEILKQML
SEERVVPHLPLAKIWEWLVGVACRQTGHQRLKAIHLLLRIVRCCGHSDLCDLALLKPLWQLFTHMEYGLFEDVTQPGILLPLHRALTELF

--------------------------------------------------------------
>In-frame_ENST00000401468_ENST00000381638_TCGA-55-8087-01A_VPS53_chr17_600658_-_ZZEF1_chr17_4027345_length(transcript)=11262nt_BP=285nt
ATGATGGAGGAGGAGGAACTGGAGTTCGTGGAGGAGCTGGAAGCCGTGCTGCAGCTCACGCCCGAGGTGCAGCTGGCCATCGAGCAGGTG
TTTCCAAGCCAGGACCCTCTAGATCGAGCAGATTTCAATGCTGTTGAGTATATCAATACCCTGTTCCCAACCGAGCAATCTCTGGCGAAC
ATAGACGAAGTCGTGAACAAAATTAGGCTGAAAATAAGGAGACTGGATGACAATATTCGAACTGTTGTAAGAGGTCAGACGAACGTGGGG
CAGGATGGACGGCAAGCCTTTGCCCAGTTTGATGCTGAGGGTGATGGGACAGTTGATGCCGAGAACATGTTGGAGGCCCTCAAGAATTCC
AGTGGAGCTAATCTTCAGGGGGAGCTGAGCCACATCATCAGACAACTACAGGCCTGCTCTCTGGTTCCAGGTTTCACAGACATATTTTCA
GAGTCGAAGGAGGGCCTTGATATTCACTCGTCAATGATACTGCGCTTCCTGCACCGCAATCGGCTCTCCAGCGCGGTGATGCCCTACCCG
ATGCTGGAGCACTGCAATAACATGTGCACCATGCGGTCTTCCGTCCTGAAGGAGTCTCTGGATCAGCTGGTACAAAAGGAAAAGGAAAGC
CCTGGAGATCTAACTAGAAGTCCAGAGATGGATAAACTCAAGTCAGTAGCAAAGTGCTATGCTTATATAGAAACATCCTCCAACTCGGCA
GACATTGACAAGATGACAAATGGAGAAACCTCATCCTACTGGCAGTCAGATGGCAGTGCCTGTTCACACTGGATTCGTTTAAAAATGAAG
CCAGATGTTGTGCTTAGGCACCTGTCCATTGCAGTGGCTGCCACTGACCAGAGCTACATGCCACAGCAGGTGACAGTAGCTGTAGGGAGG
AATGCCAGCGATCTTCAGGAAGTCCGAGATGTGCACATCCCCAGCAATGTCACTGGCTATGTGACGCTGCTGGAAAATGCCAACGTCAGT
CAGCTCTATGTCCAGATTAACATAAAGCGTTGTCTTAGCGATGGCTGCGACACTAGAATTCATGGTCTCAGGGCTGTTGGCTTTCAGAGA
GTTAAGAAGTCTGGGGTCTCAGTCTCAGATGCTTCTGCAATATGGTATTGGTCTCTGCTGACATCTCTGGTGACGGCTTCTATGGAGACA
AATCCCGCCTTTGTCCAGACAGTGCTGCACAATACTCAGAAGGCGCTGCGGCACATGCCTCCACTCTCTCTCTCACCAGGATCTACAGAT
TTCTCAACTTTCCTCTCCCCTAATGTGCTGGAAGAAGTGGACAGTTTCCTCATAAGGATAACTAGCTGCTGTTCTACCCCAGAGGTAGAA
CTGACTCTTCTGGCTTTTGCTCTCGCAAGAGGAAGTGTTGCCAAAGTCATGAGCTCTCTATGCACCATCACTGACCATCTGGACACGCAG
TATGATGCCTCATCCCTCATCTTGTCCATGGCGTCAGTCAGACAGAACCTGCTCCTCAAATATGGTAAACCTCTCCAGTTGACTCTTCAG
GCATGTGATGTCAAAGGAAAAGAAGATAAGTCTGGACCCGAAAACCTCCTTGTTGAACCGTGGACAAGGGATGGTTTTCTTACGGAAACT
GGAAAAACCAGAGCCAGCACTATTTTTTCTACCGGAACTGAATCTGCCTTCCAAGTTACACAGATAAGAATTATGGTTCGACGTGGTGGC
ATTGGTGCCCAGTGTGGGTTGGTGTTTGCCTATAACTCATCTTCAGATAAATTTTGTGCGGAAGAACACTTCAAAAGGTTTGAAAAATAT
GACAAATGGAAGCTTCAGGAGCTCAGGCAATTTGTAAAAAGCAGGATTGGTTGCTCATCTGATGACCTTGGAGAGGATGATCCTATTGGC
TGGTTTGAACTGGAAGAAGAATGGGATGAAGCAGATGTGAAGCTGCAACAGTGCAGAGTTGCCAAATATTTGATGGTGAAGTTCCTCTGC
ACCCGTCAGGAGTCAGCAGAGCGCTTGGGAGTGCAAGGCTTGACCATCAGTGGGTACCTCCGGCCTGCAAGAGCAGAAGCAGAACAGAGC
GTCACCTGTGCACACTGCAGAAAGGACACAGAGGAGAGTGTCTGTGGGGCCACGTTGCTCCTCAGGACCCTTCAGTTTATCCAGCAGCTC
GCCCATGACCTGGTGCAGCAGAAGGAAAGTGGCTTAAAATATAAATCTTTTCTGGACTTCGCGGGTCTTGATTTGCAGATCTTCTGGAAT
TTTTACAGTAAATTAAAGCAAAACCCGAGGGAAGAATGCGTCTCTGCCCAAACCCTGCTTCTGCAGCTACTCCAGAGCTGCTTCTCTGTG
CTGCAGGGAGATGTACTGGCTGCTTCTGAGGAGGAAAAGGCTCCAATCCAAAGCCCTAAAGGAGTAGAGGCTGCCAAGGAGCTGTACACA
CACTTGTGTGATGTGGTGGACAAGGTGGATGGAGACTCTGTGCCCATGGAGATACTAAAACAAGAAGTCAGGAATACCCTTCTCAATGGG
GCTGCCATCTTCTTTCCTAATCGACAGACCCGACGGAACCATCTCTTCACCATGATGAATGTCACCGAGCAGGAGCACAAGCAGTCCCTG
CAGCTCACTTTCCGTTCACTGTGCACGTATTTTAGTGACAAGGATCCAGGCGGCCTTCTTCTTTTACCTGAGAAGAACGACCTGGCCAAG
ATGAACATCAGTGAAGTCCTGGCGGTCATGGACACTCTCGTCTCTGTTGCTGCTCGAGAGTGCGAGCTGTTAATGCTCAGTGGGGCCCCA
GGGGAGGTGGGCTCTGTGCTCTTCTCCCTGTTCTGGTCCGTCCAAGGCAGCCTGCTATCCTGGTGCTACCTGCAGCTGAAGAGCACGGAC
TCTGGAGCCAAAGATCTTGCCGTGGACCTTATTGAAAAATATGTGGGCCAGTTTCTGGCAAGCATGAGAGCGATTTTGGAATCCCTTTTC
TCACAGTACAGTGGAAAAACCATAGTAGAAAGATTATGTAACTCAGTGTTTTCAATGGCAGCTCGTCAACTGGTTATCTTCCTGCTGGAC
TTCTGCACTTTAGACATCCCACACTGCGTGCTCTTGAGAGAGTTCAGCGTCCTCACAGAACTCCTGAAGAAGCTCTGTAGTGGCCCCGAA
GGAGGACTGAGGAAGCTGGATGTTGAGACCTGGCAACAGGAACAGCCTGTGGTGTTACATACGTGGACGAAGGAATCTGCCCACAACTAT
GAAAATAATTGCCATGAGGTATCCGTCTTTGTTAGCCCAGGGGCAACCTATTTTGAAGTGGAATTCGATGACAGGTGTGAAACTGAAAAA
AGGTATGATTATCTGGAATTTACCGACGCTAGAGGTCGGAAAACACGCTATGACACAAAAGTTGGCACTGATAAATGGCCCAAGAAAGTG
ACCTTCAAGGCCGGTCCTCGGTTGCAGTTCCTCTTTCACTCTGACAGCAGTCACAACGAATGGGGCTACAAATTCACTGTCACTGCCTGT
GGGCTGCCCGATGTTGCCGTGTCTTGGGGGCTGGATTTACAGCTCCTCGTCTCCCGGCTGATGGGACGCCTGGCTTCCCAGTGCATGGCG
CTCAAGTCTGTGCGCCAGTTAGGAAGTAACATGGTAGTACCTCAGGCAAAAATGGCATTAGTCCTAAGCTCCCCACTGTGGAAACCTGTC
TTCAGGCATCAGGTTTGTCCAGAGTTGGAATTAGAAGCAAGCTGGCCCACTCACCCACACCGGAATAGTAAGGAGGTTAAGAACATTCCT
GACGACCCCTGCCGCCATTTTCTTCTTGATTTTGCCCAGTCAGAGCCTGCTCAGAACTTCTGTGGGCCATATTCAGAACTTTTCAAAGGA
TTCATACAGGCATGTAGAAAACAGGCCCCAAAGACAGATATAGTTGCTGGTTCCACTATTGATCAAGCTGTGAACGCCACCTTTGCTGCT
CTGGTGTATCGCACTCCAGATTTATATGAGAAGCTGCAAAAATATGTCAACAGTGGGGGCAAAATAGCCCTGAGTGAAGAGTTTGCCCAG
GTTTATTCCTTGGCAGATGGGATTCGAATATGGATGTTAGAGATGAAGCAGAAGTCCCTGATGAGCCTGGGGAATGAAGCAGAAGAAAAA
CATAGTTCAGAAGCTACTGAGGTGAACCCTGAGAGCCTGGCAAAAGAGTGCATTGAGAAGAGTCTTCTATTACTAAAATTTCTGCCCACG
GGCATAAGTTCAAAAGAAAGCTGCGAAAAGTTGGAGACTGCTGATGAAACCAGTCATCTCCAGCCACTCAACAAGCGTCAGAGGACAAGC
TCTGTGGTGGAAGAGCATTTCCAAGCCTCAGTATCTCCCACTGAAGCCGCACCCCCTGCCACAGGAGACCAGAGTCCTGGCCTGGGCACC
CAGCCAAAGCTGCCATCCAGCAGTGGCCTTCCTGCTGCAGACGTGTCCCCTGCCACAGCTGAAGAGCCCTTGTCACCTTCCACACCCACC
CGCCGGCCTCCCTTCACCCGAGGGCGACTCCGGCTGCTCTCCTTTCGATCCATGGAGGAGGCCAGACTGGTGCCCACAGTGAAAGAGAAA
TACCCTGTGCTGAAGGACGTCATGGACTTCATTAAGGATCAGTCGCTCTCGCACAGGAGTGTTGTGAAGGTTCTTTCCCTGAGGAAAGCC
CAGGCCCAGAGCATCCTGGAAGTCCTGAAGATAACTCAGCACTGTGCAGAATCCCTTGGGCAGCCCCACTGCTTCCATCCACCTTTCATA
CTTTTCCTGTTGGAACTTCTGACCTGTCAGAAAGATTTTACCAATTATTTTGGACACCTGGAAGGCTGTGGTGCTGATCTACACAAAGAA
ATTCGAGACACTTACTATCAACTTGTTCTGTTTTTGGTCAAAGCAGTTAAAGGATTTAGTAGCCTAAATGACAGGTCCTTGCTCCCTGCC
TTATCCTGTGTTCAGACAGCCCTGCTTCATCTTTTGGATATGGGCTGGGAACCCAATGATCTCGCCTTCTTTGTTGATATTCAGTTACCA
GATCTCCTCATGAAAATGTCACAGGAGAATATAAGTGTCCATGACAGTGTGATCAGCCAATGGAGTGAAGAAGATGAGCTTGCTGATGCC
AAGCAGAATTCAGAATGGATGGATGAGTGTCAGGATGGCATGTTTGAGGCCTGGTATGAAAAAATAGCCCAGGAAGATCCAGAGAAGCAG
AGGAAAATGCACATGTTCATTGCTCGCTACTGTGACCTGTTAAATGTGGACATCTCTTGTGATGGGTGTGATGAGATTGCCCCCTGGCAT
CGATACCGCTGTCTGCAGTGCAGCGACATGGATCTCTGCAAAACTTGCTTCCTAGGTGGGGTGAAGCCTGAGGGCCACGGAGACGACCAT
GAAATGGTCAACATGGAGTTTACCTGTGACCACTGCCAGGGTTTGATCATAGGCCGGAGGATGAACTGCAATGTTTGCGATGACTTTGAT
CTTTGCTACGGATGCTATGCAGCGAAGAAATACTCCTACGGCCATTTGCCTACCCACAGCATCACGGCCCACCCAATGGTAACCATTCGG
ATCAGTGACCGGCAGAGGCTCATCCAGCCATATATCCATAACTACTCCTGGCTGCTCTTTGCTGCCCTGGCTCTCTATAGCGCCCACCTG
GCCAGTGCAGAGGATGTGGATGGGGAGAAGCTGGACCCCCAGACGCGCAGCAGTGCCACCACCCTGCGGAGCCAGTGCATGCAGCTCGTC
GGGGACTGTCTGATGAAGGCTCATCAGGGAAAAGGCCTTAAAGCTCTAGCTTTGCTGGGTGTATTGCCAGATGGGGACTCGAGCCTAGAA
GATCAGGCCCTACCAGTCACTGTGCCCACCGGAGCGTCAGAGGAGCAGCTAGAGAAGAAAGCTGTCCAGGGTGCTGAGCTGTCAGAAGCA
GGCAATGGAAAGAGAGCTGTTCATGAGGAAATCAGACCTGTAGATTTCAAGCAGAGAAATAAGGCAGATAAAGGTGTATCATTATCGAAG
GATCCTTCATGCCAGACCCAAATTTCAGATTCACCTGCAGATGCTAGCCCACCTACAGGACTTCCAGATGCTGAAGATTCAGAAGTGTCA
TCTCAGAAGCCCATAGAGGAAAAAGCAGTTACTCCAAGCCCTGAGCAAGTGTTTGCTGAGTGTTCCCAGAAGAGGATTTTGGGATTACTA
GCAGCCATGTTACCTCCCTTAAAGTCGGGCCCCACGGTTCCCCTGATAGACCTGGAGCACGTCCTTCCACTCATGTTTCAGGTTGTCATC
TCAAACGCAGGCCACCTGAATGAAACCTACCATCTCACCCTGGGTCTTCTCGGCCAGTTAATTATCCGTCTTTTGCCAGCAGAGGTAGAC
GCCGCAGTGATCAAAGTCCTCTCAGCCAAACACAACCTGTTTGCTGCAGGGGACAGTTCCATTGTGCCAGATGGCTGGAAAACCACCCAC
CTGCTCTTTAGCCTGGGAGCTGTGTGTCTGGACAGCCGGGTGGGCTTGGACTGGGCGTGCTCCATGGCAGAGATCCTGCGGTCACTCAAC
AGTGCCCCACTGTGGCGTGATGTCATTGCCACCTTCACAGACCACTGCATCAAGCAGCTGCCATTCCAGCTGAAGCACACCAACATCTTC
ACCCTGCTCGTGCTGGTTGGCTTCCCCCAGGTCCTCTGTGTGGGAACCCGCTGCGTTTATATGGATAATGCCAATGAACCCCATAATGTG
ATCATCTTGAAGCACTTTACTGAGAAGAACAGGGCTGTGATTGTTGATGTCAAAACTCGGAAGAGGAAAACAGTGAAGGACTACCAGCTG
GTCCAGAAGGGAGGAGGACAAGAGTGTGGTGACTCTCGGGCCCAGCTGAGCCAGTACTCCCAGCACTTTGCCTTTATCGCCAGTCACCTT
CTGCAAAGCAGCATGGACAGCCATTGTCCCGAGGCAGTAGAAGCAACTTGGGTCCTGTCCCTGGCCCTGAAAGGATTGTATAAAACACTA
AAGGCTCACGGTTTTGAGGAGATCCGTGCTACTTTCCTTCAGACCGATTTGCTGAAGTTGCTGGTGAAAAAGTGCAGCAAAGGGACTGGC
TTTAGTAAAACGTGGCTCCTCCGGGACCTGGAAATTTTGTCCATCATGCTGTACTCCTCAAAAAAGGAGATCAACGCTTTGGCTGAGCAC
GGAGACCTAGAGCTGGATGAGCGAGGGGACCGAGAGGAAGAGGTGGAACGGCCAGTCAGCAGCCCTGGCGACCCAGAGCAGAAAAAGCTG
GACCCCCTTGAGGGCCTGGATGAGCCCACCAGAATATGTTTCTTGATGGCTCATGATGCCCTCAATGCCCCTCTGCACATTCTCCGGGCC
ATATACGAACTGCAGATGAAAAAGACCGATTATTTCTTCCTGGAGGTTCAGAAGAGGTTTGATGGTGATGAGCTCACCACAGATGAAAGG
ATACGGTCCCTGGCTCAGCGGTGGCAGCCCAGTAAGAGTCTGAGGCTGGAAGAACAGAGCGCCAAAGCTGTGGATACAGACATGATTATC
CTGCCATGCTTGTCCCGGCCTGCACGCTGTGACCAAGCCACTGCTGAATCGAACCCTGTGACCCAGAAGCTGATCTCCAGCACAGAGAGC
GAACTGCAGCAGAGCTATGCCAAGCAGCGCCGTAGCAAGAGCGCCGCCCTCCTGCACAAGGAGCTGAACTGCAAGAGTAAGAGGGCTGTC
CGGGACTACCTCTTCCGAGTGAACGAGGCCACAGCTGTCCTGTACGCCCGCCACGTGCTTGCATCCCTGCTCGCCGAGTGGCCTAGCCAC
GTGCCAGTGAGCGAGGACATCCTGGAGCTCAGTGGCCCTGCCCATATGACCTACATTTTGGATATGTTCATGCAGCTGGAAGAAAAGCAT
GAGTGGGAGAAGATCCTGCAGAAAGTGCTCCAGGGCTGCCGAGAGGACATGCTGGGGACCATGGCCCTGGCTGCATGCCAGTTCATGGAG
GAGCCAGGAATGGAGGTGCAAGTGAGGGAGTCGAAACACCCGTATAACAACAACACCAACTTCGAGGATAAAGTTCACATTCCTGGTGCC
ATCTACCTCTCAATCAAATTCGACTCTCAGTGCAACACAGAGGAGGGCTGTGACGAGTTAGCCATGTCCAGCAGCAGTGACTTCCAGCAA
GACCGACACAGCTTCAGCGGGTCTCAGCAGAAGTGGAAAGATTTTGAACTTCCAGGAGACACTCTGTATTACCGCTTCACCTCCGACATG
AGCAACACCGAGTGGGGCTACAGATTCACCGTGACGGCCGGACACCTGGGGCGGTTCCAGACAGGATTCGAGATTTTGAAGCAGATGTTG
TCAGAAGAAAGGGTCGTGCCTCATCTCCCATTGGCAAAAATTTGGGAATGGCTGGTGGGCGTGGCCTGTCGCCAGACTGGCCATCAACGA
TTAAAAGCCATCCACTTACTTCTGAGGATTGTGCGATGCTGCGGCCACAGTGACCTGTGTGACCTTGCGCTGTTGAAGCCCCTGTGGCAG
CTCTTTACCCACATGGAGTACGGCCTGTTTGAGGACGTGACGCAGCCCGGCATCCTCCTTCCCCTGCATCGTGCCCTCACTGAGCTCTTC
TTCGTCACCGAGAACCGTGCCCAGGAGCTTGGCGTGCTGCAGGATTACCTGCTGGCCCTAACCACGGACGACCACCTTCTCCGCTGTGCG
GCACAGGCTCTGCAGAACATTGCTGCCATCAGCCTGGCCATCAACTACCCAAACAAGGCCACCCGCCTCTGGAATGTGGAGTGTTAGCCC
TTGGTGGGGCGTGCATGGGACTAGTTCATCTGCCACAGGGATTTTAGAGCAGACATCTAACCTCATTCAGGAAAACTCCTGTAGCGCCAG
TGCCCAGCTCTCCTTGAGCTGACCACTCCAGTTAGGATGCCAAGCAGCCACGTCTCCAAGAGCTCCCGTGCGTAGGCTGGACACAAGCAC
AGGCTGTAGCATGGTGAAAATAAGCCAAGCAGTGCAGAATGCCTCAGAAAGGGTGGGCAGGGGGCCCTTAAGAAGGTTCAGAGACCAGCC
TTCTCCAGAGGCTGTCACTGCAGGAGCCGTGGGCCTGGGAAGACTTGGAAGCGGCCTCTCTCAACTGGTTTCTGTCTCCGTGGAGCTGGA
ACTGCCTGCACTTGCCTTCAGAGGGAGGCACAGTCCACCCAGATCCACCTTTCCAGCAAGACCCCCAGTGGCTGCCCAGCCTGGGAGCAC
CTCTTTGCTTTTCACACCAAACCAAAACTGGCGAGAGCCCCTCCTAGCCACCAGTGATCCCCAAGCATCCAGTACAGAACCAGGCATCGA
GCTAGCTCCCTGCACGGCCGCACCCTCCCAGAGAACTCCTTGAGGAGAACAAGTGCCCTTGGGGACAGCCGGCAGGCGCCCCTGTACGTC
TGCTCATGCACCAGGCAGCACAGCCGCAGTTCCTCAGTTGTTGTTTTGACATATTTCAGTTTCCACCTCACGTTTTTAGAGCAGAACCAC
ACTGTCTCCCTGGAGGGGCTCGAGGGCATGACCGGGGACTGACCATTCTGTGAAAGGAGCAGAATGTGAGGAGCACGCGTGAGCTTATGT
ACCGTGAAGATGATCAGAGGATATCTTATTTTAAGAGTAAAAACCCACATAATTTTATTTCTGCTTGATAGTCATGGTAGTCTGTCATAC
CCACCTCTGGGACTCTGCGTGGCTGTTTGGCTGTCACTTGTAGCAATAACGACATTAGTTCTAGTCAGTGCTGTTTTACATTTTTCTTTT
GATGGGTTTAGTCTTGCCCTGGAGTGCCGATGATGATTCTCCCTCCAGAGCCACGCTTGGGAACATGAAGCAAGTCTGGCGTGTGGGCTG
CGTGCCGGCCTTAGTGGGACCCGTGGGGTTGGAGCATGCCTTTAGGGGCAGTGTCTGGGCCGAAGCACGTCCCACCACACAGTGCCAGAG
CCAGAGAAGGGGCCCCACCACCAAGGCCAAGCTTGACCAGGTCAGCATTGCCATGGCCCAGTGTGCCCCGTGGCCTCTGAAGATCCCTCT
GTGCAGGGTCTGCAGGGATCTGGATTGCAAGGGCCCAAGTCTGCAGGTCTGGAAGCATCTTCCTATAAGAGCACTTTCGCCTTCTGGGTC
AGGACTCCAAGGTGCAGCGGGCTTCACAGCCCTACAATTGGGTTCTCAGCTAAGCCCCAGAGTTCTGGTAGAACCATCCCGGGGCGGGTG
GAGGGTGGGATTTAAGGGAGACGGGAACACATGGGGCAGGTCCTGGAACTTGGTGGCCTGAGGACTGAGGCCATTGCCCTGGTGGAAAGG
CCTGGCCTGGTTCCTGTGGCTTGGGACCTGAATAGGCAGGTGCTGCTGGCTCCGTAGAAACCCTTTTCCCATCTTTTGCTCTTTGCCAAA
CCTACCTTGCTTTGGGAGCTGCCTGCACCACCCCAGAGAAGGCCCCACCTTCTTCATCCCTCAGACCCGAGGAGGCCTCCCAGTAAGGAG
TTTCCCAAGAGGGGACTCACAGGAAACAAGTCTTAGTGCTTGGGAGGGAGGCCCCGCTGCGTGCTCAGACTCACAGCCAACCTGGAAGGT
AGACGAGATAGCGCCACCCACGCCCCTCCACACCCCAGACTCCGAGTAAAGCGGGCGGTAGGGCCGGAGTCACCTCCCCTATGGCAGTGG
CCGCCGCTGTACTCCATCCTCCCGTCAGGAAGATCAGCTGTAAATAAACGCTGGGCTCCCCAGAGCACCTGTCCGCCCACTGCCCTTGCT
GTTCTGGGATCTTCGCTGCAGTTCACGGGAAACAAGCCTGAGTCCGCTCGCACCCGCGGCTGCTCTCCCGGCTCGGCCCGGCCGCCTCTG
TCTCCGGCCACCGGGTGGCGCTGCCGAGCCAGAGCCGCCGCGTCCCGGCGCTTTCCAGGAGCCCCAGGCCCGGAGGAGGCGAAGCCCGCA
GAGCAAAGGTGGAAACACGTGCCTACGCTGTAAAGAAATCCTGTTCCAGAGCATACCTGTTGTACAAACAGACACTGTTCCTAACGAGAG
GAGTGACGTATTTTCATCACCGTTTTTAATTTGTTTTCTTACGGGTTTACGATTTTGAATTTTTCTTATTTGGTTGAAAGAATTTTGATT
CTATCAGCCTGAGTGAGTTCAGCCTGTAAAAAGGATGTTAAGCTGTGGGTAAAATATGCAAACGAAAAGAAATATATTGTACAAATTCTA

>In-frame_ENST00000401468_ENST00000381638_TCGA-55-8087-01A_VPS53_chr17_600658_-_ZZEF1_chr17_4027345_length(amino acids)=2938AA_start in transcript=0_stop in transcript=8816
MMEEEELEFVEELEAVLQLTPEVQLAIEQVFPSQDPLDRADFNAVEYINTLFPTEQSLANIDEVVNKIRLKIRRLDDNIRTVVRGQTNVG
QDGRQAFAQFDAEGDGTVDAENMLEALKNSSGANLQGELSHIIRQLQACSLVPGFTDIFSESKEGLDIHSSMILRFLHRNRLSSAVMPYP
MLEHCNNMCTMRSSVLKESLDQLVQKEKESPGDLTRSPEMDKLKSVAKCYAYIETSSNSADIDKMTNGETSSYWQSDGSACSHWIRLKMK
PDVVLRHLSIAVAATDQSYMPQQVTVAVGRNASDLQEVRDVHIPSNVTGYVTLLENANVSQLYVQINIKRCLSDGCDTRIHGLRAVGFQR
VKKSGVSVSDASAIWYWSLLTSLVTASMETNPAFVQTVLHNTQKALRHMPPLSLSPGSTDFSTFLSPNVLEEVDSFLIRITSCCSTPEVE
LTLLAFALARGSVAKVMSSLCTITDHLDTQYDASSLILSMASVRQNLLLKYGKPLQLTLQACDVKGKEDKSGPENLLVEPWTRDGFLTET
GKTRASTIFSTGTESAFQVTQIRIMVRRGGIGAQCGLVFAYNSSSDKFCAEEHFKRFEKYDKWKLQELRQFVKSRIGCSSDDLGEDDPIG
WFELEEEWDEADVKLQQCRVAKYLMVKFLCTRQESAERLGVQGLTISGYLRPARAEAEQSVTCAHCRKDTEESVCGATLLLRTLQFIQQL
AHDLVQQKESGLKYKSFLDFAGLDLQIFWNFYSKLKQNPREECVSAQTLLLQLLQSCFSVLQGDVLAASEEEKAPIQSPKGVEAAKELYT
HLCDVVDKVDGDSVPMEILKQEVRNTLLNGAAIFFPNRQTRRNHLFTMMNVTEQEHKQSLQLTFRSLCTYFSDKDPGGLLLLPEKNDLAK
MNISEVLAVMDTLVSVAARECELLMLSGAPGEVGSVLFSLFWSVQGSLLSWCYLQLKSTDSGAKDLAVDLIEKYVGQFLASMRAILESLF
SQYSGKTIVERLCNSVFSMAARQLVIFLLDFCTLDIPHCVLLREFSVLTELLKKLCSGPEGGLRKLDVETWQQEQPVVLHTWTKESAHNY
ENNCHEVSVFVSPGATYFEVEFDDRCETEKRYDYLEFTDARGRKTRYDTKVGTDKWPKKVTFKAGPRLQFLFHSDSSHNEWGYKFTVTAC
GLPDVAVSWGLDLQLLVSRLMGRLASQCMALKSVRQLGSNMVVPQAKMALVLSSPLWKPVFRHQVCPELELEASWPTHPHRNSKEVKNIP
DDPCRHFLLDFAQSEPAQNFCGPYSELFKGFIQACRKQAPKTDIVAGSTIDQAVNATFAALVYRTPDLYEKLQKYVNSGGKIALSEEFAQ
VYSLADGIRIWMLEMKQKSLMSLGNEAEEKHSSEATEVNPESLAKECIEKSLLLLKFLPTGISSKESCEKLETADETSHLQPLNKRQRTS
SVVEEHFQASVSPTEAAPPATGDQSPGLGTQPKLPSSSGLPAADVSPATAEEPLSPSTPTRRPPFTRGRLRLLSFRSMEEARLVPTVKEK
YPVLKDVMDFIKDQSLSHRSVVKVLSLRKAQAQSILEVLKITQHCAESLGQPHCFHPPFILFLLELLTCQKDFTNYFGHLEGCGADLHKE
IRDTYYQLVLFLVKAVKGFSSLNDRSLLPALSCVQTALLHLLDMGWEPNDLAFFVDIQLPDLLMKMSQENISVHDSVISQWSEEDELADA
KQNSEWMDECQDGMFEAWYEKIAQEDPEKQRKMHMFIARYCDLLNVDISCDGCDEIAPWHRYRCLQCSDMDLCKTCFLGGVKPEGHGDDH
EMVNMEFTCDHCQGLIIGRRMNCNVCDDFDLCYGCYAAKKYSYGHLPTHSITAHPMVTIRISDRQRLIQPYIHNYSWLLFAALALYSAHL
ASAEDVDGEKLDPQTRSSATTLRSQCMQLVGDCLMKAHQGKGLKALALLGVLPDGDSSLEDQALPVTVPTGASEEQLEKKAVQGAELSEA
GNGKRAVHEEIRPVDFKQRNKADKGVSLSKDPSCQTQISDSPADASPPTGLPDAEDSEVSSQKPIEEKAVTPSPEQVFAECSQKRILGLL
AAMLPPLKSGPTVPLIDLEHVLPLMFQVVISNAGHLNETYHLTLGLLGQLIIRLLPAEVDAAVIKVLSAKHNLFAAGDSSIVPDGWKTTH
LLFSLGAVCLDSRVGLDWACSMAEILRSLNSAPLWRDVIATFTDHCIKQLPFQLKHTNIFTLLVLVGFPQVLCVGTRCVYMDNANEPHNV
IILKHFTEKNRAVIVDVKTRKRKTVKDYQLVQKGGGQECGDSRAQLSQYSQHFAFIASHLLQSSMDSHCPEAVEATWVLSLALKGLYKTL
KAHGFEEIRATFLQTDLLKLLVKKCSKGTGFSKTWLLRDLEILSIMLYSSKKEINALAEHGDLELDERGDREEEVERPVSSPGDPEQKKL
DPLEGLDEPTRICFLMAHDALNAPLHILRAIYELQMKKTDYFFLEVQKRFDGDELTTDERIRSLAQRWQPSKSLRLEEQSAKAVDTDMII
LPCLSRPARCDQATAESNPVTQKLISSTESELQQSYAKQRRSKSAALLHKELNCKSKRAVRDYLFRVNEATAVLYARHVLASLLAEWPSH
VPVSEDILELSGPAHMTYILDMFMQLEEKHEWEKILQKVLQGCREDMLGTMALAACQFMEEPGMEVQVRESKHPYNNNTNFEDKVHIPGA
IYLSIKFDSQCNTEEGCDELAMSSSSDFQQDRHSFSGSQQKWKDFELPGDTLYYRFTSDMSNTEWGYRFTVTAGHLGRFQTGFEILKQML
SEERVVPHLPLAKIWEWLVGVACRQTGHQRLKAIHLLLRIVRCCGHSDLCDLALLKPLWQLFTHMEYGLFEDVTQPGILLPLHRALTELF

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Fusion Gene PPI Analysis for VPS53-ZZEF1


check button Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in

ChiPPI page.


check button Protein-protein interactors with each fusion partner protein in wild-type (BIOGRID-3.4.160)
HgeneHgene's interactorsTgeneTgene's interactors


check button - Retained PPIs in in-frame fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenStill interaction with


check button - Lost PPIs in in-frame fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenInteraction lost with


check button - Retained PPIs, but lost function due to frame-shift fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenInteraction lost with


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Related Drugs for VPS53-ZZEF1


check button Drugs targeting genes involved in this fusion gene.
(DrugBank Version 5.1.8 2021-05-08)
PartnerGeneUniProtAccDrugBank IDDrug nameDrug activityDrug typeDrug status

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Related Diseases for VPS53-ZZEF1


check button Diseases associated with fusion partners.
(DisGeNet 4.0)
PartnerGeneDisease IDDisease name# pubmedsSource
HgeneVPS53C4014488PONTOCEREBELLAR HYPOPLASIA, TYPE 2E3CTD_human;GENOMICS_ENGLAND;UNIPROT
HgeneVPS53C0266468Congenital pontocerebellar hypoplasia1GENOMICS_ENGLAND
HgeneVPS53C0424605Developmental delay (disorder)1GENOMICS_ENGLAND
HgeneVPS53C0557874Global developmental delay1GENOMICS_ENGLAND
HgeneVPS53C3714756Intellectual Disability1GENOMICS_ENGLAND