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Fusion Gene Summary | |
Fusion Gene ORF analysis | |
Fusion Genomic Features | |
Fusion Protein Features | |
Fusion Gene Sequence | |
Fusion Gene PPI analysis | |
Related Drugs | |
Related Diseases |
Fusion gene:XKR4-KHDRBS3 (FusionGDB2 ID:98936) |
Fusion Gene Summary for XKR4-KHDRBS3 |
Fusion gene summary |
Fusion gene information | Fusion gene name: XKR4-KHDRBS3 | Fusion gene ID: 98936 | Hgene | Tgene | Gene symbol | XKR4 | KHDRBS3 | Gene ID | 114786 | 10656 |
Gene name | XK related 4 | KH RNA binding domain containing, signal transduction associated 3 | |
Synonyms | XRG4 | Etle|SALP|SLM-2|SLM2|T-STAR|TSTAR|etoile | |
Cytomap | 8q12.1 | 8q24.23 | |
Type of gene | protein-coding | protein-coding | |
Description | XK-related protein 4X Kell blood group precursor-related family, member 4X-linked Kx blood group related 4XK, Kell blood group complex subunit-related family, member 4 | KH domain-containing, RNA-binding, signal transduction-associated protein 3KH domain containing, RNA binding, signal transduction associated 3RNA-binding protein T-StarSam68-like phosphotyrosine protein, T-STARsam68-like mammalian protein 2 | |
Modification date | 20200313 | 20200313 | |
UniProtAcc | . | O75525 | |
Ensembl transtripts involved in fusion gene | ENST00000327381, ENST00000518261, | ENST00000355849, ENST00000520981, ENST00000522578, | |
Fusion gene scores | * DoF score | 5 X 6 X 3=90 | 11 X 10 X 10=1100 |
# samples | 5 | 14 | |
** MAII score | log2(5/90*10)=-0.84799690655495 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0 | log2(14/1100*10)=-2.97400479146706 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0 | |
Context | PubMed: XKR4 [Title/Abstract] AND KHDRBS3 [Title/Abstract] AND fusion [Title/Abstract] | ||
Most frequent breakpoint | XKR4(56015855)-KHDRBS3(136594119), # samples:1 | ||
Anticipated loss of major functional domain due to fusion event. |
* DoF score (Degree of Frequency) = # partners X # break points X # cancer types ** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10) |
Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez |
Partner | Gene | GO ID | GO term | PubMed ID |
Fusion gene breakpoints across XKR4 (5'-gene) * Click on the image to open the UCSC genome browser with custom track showing this image in a new window. |
Fusion gene breakpoints across KHDRBS3 (3'-gene) * Click on the image to open the UCSC genome browser with custom track showing this image in a new window. |
Fusion gene information from two resources (ChiTars 5.0 and ChimerDB 4.0) * All genome coordinats were lifted-over on hg19. * Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser. |
Source | Disease | Sample | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand |
ChiTaRS5.0 | N/A | BC106028 | XKR4 | chr8 | 56015855 | + | KHDRBS3 | chr8 | 136594119 | + |
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Fusion Gene ORF analysis for XKR4-KHDRBS3 |
Open reading frame (ORF) analsis of fusion genes based on Ensembl gene isoform structure. * Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser. |
ORF | Henst | Tenst | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand |
In-frame | ENST00000327381 | ENST00000355849 | XKR4 | chr8 | 56015855 | + | KHDRBS3 | chr8 | 136594119 | + |
5CDS-intron | ENST00000327381 | ENST00000520981 | XKR4 | chr8 | 56015855 | + | KHDRBS3 | chr8 | 136594119 | + |
5CDS-intron | ENST00000327381 | ENST00000522578 | XKR4 | chr8 | 56015855 | + | KHDRBS3 | chr8 | 136594119 | + |
intron-3CDS | ENST00000518261 | ENST00000355849 | XKR4 | chr8 | 56015855 | + | KHDRBS3 | chr8 | 136594119 | + |
intron-intron | ENST00000518261 | ENST00000520981 | XKR4 | chr8 | 56015855 | + | KHDRBS3 | chr8 | 136594119 | + |
intron-intron | ENST00000518261 | ENST00000522578 | XKR4 | chr8 | 56015855 | + | KHDRBS3 | chr8 | 136594119 | + |
intron-intron | ENST00000327381 | ENST00000355849 | XKR4 | chr8 | 56178409 | + | KHDRBS3 | chr8 | 136663300 | + |
intron-intron | ENST00000327381 | ENST00000520981 | XKR4 | chr8 | 56178409 | + | KHDRBS3 | chr8 | 136663300 | + |
intron-intron | ENST00000327381 | ENST00000522578 | XKR4 | chr8 | 56178409 | + | KHDRBS3 | chr8 | 136663300 | + |
intron-intron | ENST00000518261 | ENST00000355849 | XKR4 | chr8 | 56178409 | + | KHDRBS3 | chr8 | 136663300 | + |
intron-intron | ENST00000518261 | ENST00000520981 | XKR4 | chr8 | 56178409 | + | KHDRBS3 | chr8 | 136663300 | + |
intron-intron | ENST00000518261 | ENST00000522578 | XKR4 | chr8 | 56178409 | + | KHDRBS3 | chr8 | 136663300 | + |
ORFfinder result based on the fusion transcript sequence of in-frame fusion genes. |
Henst | Tenst | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand | Seq length (transcript) | BP loci (transcript) | Predicted start (transcript) | Predicted stop (transcript) | Seq length (amino acids) |
ENST00000327381 | XKR4 | chr8 | 56015855 | + | ENST00000355849 | KHDRBS3 | chr8 | 136594119 | + | 1862 | 906 | 100 | 1335 | 411 |
DeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated. |
Henst | Tenst | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand | No-coding score | Coding score |
ENST00000327381 | ENST00000355849 | XKR4 | chr8 | 56015855 | + | KHDRBS3 | chr8 | 136594119 | + | 0.010786226 | 0.98921376 |
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Fusion Genomic Features for XKR4-KHDRBS3 |
FusionAI prediction of the potential fusion gene breakpoint based on the pre-mature RNA sequence context (+/- 5kb of individual partner genes, total 20kb length sequence). FusionAI is a fusion gene breakpoint classifier based on convolutional neural network by comparing the fusion positive and negative sequence context of ~ 20K fusion gene data. From here, we can have the relative potentency of the 20K genomic sequence how individual sequnce will be likely used as the gene fusion breakpoints. |
Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand | 1-p | p (fusion gene breakpoint) |
XKR4 | chr8 | 56015854 | + | KHDRBS3 | chr8 | 136594120 | + | 0.000933025 | 0.999067 |
XKR4 | chr8 | 56015854 | + | KHDRBS3 | chr8 | 136594120 | + | 0.000933025 | 0.999067 |
Distribution of 44 human genomic features loci across 20kb length fusion breakpoint regions. We integrated a total of 44 different types of human genomic feature loci information across five big categories including virus integration sites, repeats, structural variants, chromatin states, and gene expression regulation. More details are in help page. |
Distribution of 44 human genomic features loci across 20kb length fusion breakpoint regions that are ovelapped with the top 1% feature importance score regions. More details are in help page. |
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Fusion Protein Features for XKR4-KHDRBS3 |
Go to FGviewer for the breakpoints of chr8:56015855-chr8:136594119 - FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels. |
Main function of each fusion partner protein. (from UniProt) |
Hgene | Tgene |
. | KHDRBS3 |
FUNCTION: Might normally function as a transcriptional repressor. EWS-fusion-proteins (EFPS) may play a role in the tumorigenic process. They may disturb gene expression by mimicking, or interfering with the normal function of CTD-POLII within the transcription initiation complex. They may also contribute to an aberrant activation of the fusion protein target genes. | FUNCTION: RNA-binding protein that plays a role in the regulation of alternative splicing and influences mRNA splice site selection and exon inclusion. Binds preferentially to the 5'-[AU]UAAA-3' motif in vitro. Binds optimally to RNA containing 5'-[AU]UAA-3' as a bipartite motif spaced by more than 15 nucleotides. Binds poly(A). RNA-binding abilities are down-regulated by tyrosine kinase PTK6 (PubMed:10564820, PubMed:19561594, PubMed:26758068). Involved in splice site selection of vascular endothelial growth factor (PubMed:15901763). In vitro regulates CD44 alternative splicing by direct binding to purine-rich exonic enhancer (By similarity). Can regulate alternative splicing of neurexins NRXN1-3 in the laminin G-like domain 6 containing the evolutionary conserved neurexin alternative spliced segment 4 (AS4) involved in neurexin selective targeting to postsynaptic partners such as neuroligins and LRRTM family members (PubMed:26758068). Targeted, cell-type specific splicing regulation of NRXN1 at AS4 is involved in neuronal glutamatergic synapse function and plasticity (By similarity). May regulate expression of KHDRBS2/SLIM-1 in defined brain neuron populations by modifying its alternative splicing (By similarity). Can bind FABP9 mRNA (By similarity). May play a role as a negative regulator of cell growth. Inhibits cell proliferation. {ECO:0000250|UniProtKB:Q9JLP1, ECO:0000250|UniProtKB:Q9R226, ECO:0000269|PubMed:10564820, ECO:0000269|PubMed:15901763, ECO:0000269|PubMed:19561594, ECO:0000269|PubMed:26758068}.; FUNCTION: (Microbial infection) Involved in post-transcriptional regulation of HIV-1 gene expression. {ECO:0000269|PubMed:11741900}. |
Retention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at * Minus value of BPloci means that the break pointn is located before the CDS. |
- In-frame and retained protein feature among the 13 regional features. |
Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Protein feature | Protein feature note |
Hgene | XKR4 | chr8:56015855 | chr8:136594119 | ENST00000327381 | + | 1 | 3 | 114_134 | 268.6666666666667 | 651.0 | Transmembrane | Helical |
Hgene | XKR4 | chr8:56015855 | chr8:136594119 | ENST00000327381 | + | 1 | 3 | 144_164 | 268.6666666666667 | 651.0 | Transmembrane | Helical |
Hgene | XKR4 | chr8:56015855 | chr8:136594119 | ENST00000327381 | + | 1 | 3 | 248_268 | 268.6666666666667 | 651.0 | Transmembrane | Helical |
Tgene | KHDRBS3 | chr8:56015855 | chr8:136594119 | ENST00000355849 | 4 | 9 | 250_261 | 203.66666666666666 | 347.0 | Compositional bias | Note=Pro-rich | |
Tgene | KHDRBS3 | chr8:56015855 | chr8:136594119 | ENST00000355849 | 4 | 9 | 266_316 | 203.66666666666666 | 347.0 | Compositional bias | Note=Tyr-rich |
- In-frame and not-retained protein feature among the 13 regional features. |
Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Protein feature | Protein feature note |
Hgene | XKR4 | chr8:56015855 | chr8:136594119 | ENST00000327381 | + | 1 | 3 | 306_326 | 268.6666666666667 | 651.0 | Transmembrane | Helical |
Hgene | XKR4 | chr8:56015855 | chr8:136594119 | ENST00000327381 | + | 1 | 3 | 331_351 | 268.6666666666667 | 651.0 | Transmembrane | Helical |
Hgene | XKR4 | chr8:56015855 | chr8:136594119 | ENST00000327381 | + | 1 | 3 | 365_385 | 268.6666666666667 | 651.0 | Transmembrane | Helical |
Hgene | XKR4 | chr8:56015855 | chr8:136594119 | ENST00000327381 | + | 1 | 3 | 396_418 | 268.6666666666667 | 651.0 | Transmembrane | Helical |
Hgene | XKR4 | chr8:56015855 | chr8:136594119 | ENST00000327381 | + | 1 | 3 | 428_448 | 268.6666666666667 | 651.0 | Transmembrane | Helical |
Hgene | XKR4 | chr8:56015855 | chr8:136594119 | ENST00000327381 | + | 1 | 3 | 457_477 | 268.6666666666667 | 651.0 | Transmembrane | Helical |
Hgene | XKR4 | chr8:56015855 | chr8:136594119 | ENST00000327381 | + | 1 | 3 | 487_507 | 268.6666666666667 | 651.0 | Transmembrane | Helical |
Tgene | KHDRBS3 | chr8:56015855 | chr8:136594119 | ENST00000355849 | 4 | 9 | 61_127 | 203.66666666666666 | 347.0 | Domain | Note=KH | |
Tgene | KHDRBS3 | chr8:56015855 | chr8:136594119 | ENST00000355849 | 4 | 9 | 1_160 | 203.66666666666666 | 347.0 | Region | Involved in homodimerization |
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Fusion Gene Sequence for XKR4-KHDRBS3 |
For in-frame fusion transcripts, we provide the fusion transcript sequences and fusion amino acid sequences. To have fusion amino acid sequence, we ran ORFfinder and chose the longest ORF among the all predicted ones. |
>In-frame_ENST00000327381_ENST00000355849_BC106028_XKR4_chr8_56015855_+_KHDRBS3_chr8_136594119_length(transcript)=1862nt_BP=906nt CTGGGAGGGAAGCCGGGGCGAGGCGAGGAGGTGGCGGGAGGAGGAGACAGCGGGGAAAGGTGTCAGATAAAGGAGGGCTCTCCTCCGGTG TGGAGGCATCATGGCCGCTAAATCAGACGGGAGGCTGAAAATGAAGAAAAGCAGCGACGTGGCGTTCACCCCGCTGCAGAACTCGGACCA CTCGGGCTCGGTGCAGGGATTGGCTCCAGGCTTGCCGTCGGGGTCGGGAGCCGAGGACGAGGAGGCGGCCGGGGGCGGCTGCTGCCCGGA CGGCGGCGGCTGCTCGCGCTGCTGCTGCTGCTGCGCCGGGAGTGGCGGCTCCGCGGGCTCGGGCGGCTCCGGCGGCGTCGCCGGCCCGGG CGGCGGCGGGGCGGGCTCGGCTGCGCTGTGCCTGCGCCTGGGCAGGGAGCAGCGGCGCTACTCACTGTGGGACTGCCTCTGGATCCTGGC CGCCGTGGCCGTGTACTTCGCGGACGTGGGCACAGACGTCTGGCTCGCCGTGGACTACTACCTGCGCGGCCAGCGCTGGTGGTTCGGGCT CACGCTCTTCTTCGTGGTGCTCGGCTCTCTGTCGGTGCAAGTGTTCAGCTTCCGCTGGTTTGTGCACGATTTCAGCACCGAGGACAGCGC CACGGCCGCTGCTGCCTCCAGCTGCCCGCAGCCTGGAGCCGATTGCAAGACGGTGGTCGGCGGTGGGTCTGCAGCCGGGGAAGGCGAGGC TCGTCCTTCCACGCCGCAAAGGCAAGCATCTAACGCCAGCAAGAGCAACATCGCCGCGGCCAACAGCGGCAGCAACAGCAGCGGGGCTAC CCGGGCCAGTGGCAAGCACAGGTCTGCGTCCTGCTCCTTCTGCATCTGGCTCCTGCAGTCACTCATCCACATCTTGCAGCTCGGGCAAAT CTGGAGGGGAAGGGGAGGAGTTACAGCCCGGCCAGTTGGAGTTGTAGTACCACGAGGGACGCCAACTCCCAGAGGAGTCCTGTCCACCCG AGGGCCAGTGAGTCGGGGAAGAGGACTTCTCACTCCCAGAGCAAGAGGAGTCCCCCCAACTGGGTACAGACCTCCACCGCCACCCCCGAC ACAAGAGACTTATGGAGAATATGACTATGATGATGGATATGGCACTGCTTATGATGAACAGAGTTATGATTCCTATGATAACAGCTATAG CACCCCAGCCCAAAGTGGTGCTGATTACTATGATTACGGACATGGACTCAGTGAGGAGACTTATGATTCCTACGGGCAAGAAGAGTGGAC TAACTCAAGACACAAGGCACCTTCAGCGAGGACAGCAAAGGGCGTCTACAGAGACCAGCCATATGGCAGATACTGATTGTACTGTCTGAT GTTGTGAAATAGCCAATCTCCACCAGTCCTGTATACTGTTCAAAGTAATTTTTTTCTATGAACAATCCCTTTTTAAATAAATCAGAATGC TTAAAATCTGAATGGATGGAACTTAAAACTACTTTGTTGAAACATCAACCTGGGCAGAAAAAAAAAAAAAAAGACATGTAAAATTTTGTT ATTTCCAGTCTGTATATGAAAAAATAGGTCATCAAAAGGAAAAAAAATAACTTTGATTAACTAGTGTTAAACAAAAAATAGGTTTACTAA ATATGTTAATCTATTCTTTTAACATAAGCCTCACCTTTCATTTTAAAGGTTTCCATAGAATTTAGTTATTTTATCTTTCAGCCATATGCT AGTTTTTTTTTCTCTTGCCAACATGCGTAAAAAGGGAAGCCAATTACAAGTGCAAATAATGTGGTATTCTTTTGTAACTCAAGTCTTGAA >In-frame_ENST00000327381_ENST00000355849_BC106028_XKR4_chr8_56015855_+_KHDRBS3_chr8_136594119_length(amino acids)=411AA_start in transcript=100_stop in transcript=1335 MAAKSDGRLKMKKSSDVAFTPLQNSDHSGSVQGLAPGLPSGSGAEDEEAAGGGCCPDGGGCSRCCCCCAGSGGSAGSGGSGGVAGPGGGG AGSAALCLRLGREQRRYSLWDCLWILAAVAVYFADVGTDVWLAVDYYLRGQRWWFGLTLFFVVLGSLSVQVFSFRWFVHDFSTEDSATAA AASSCPQPGADCKTVVGGGSAAGEGEARPSTPQRQASNASKSNIAAANSGSNSSGATRASGKHRSASCSFCIWLLQSLIHILQLGQIWRG RGGVTARPVGVVVPRGTPTPRGVLSTRGPVSRGRGLLTPRARGVPPTGYRPPPPPPTQETYGEYDYDDGYGTAYDEQSYDSYDNSYSTPA -------------------------------------------------------------- |
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Fusion Gene PPI Analysis for XKR4-KHDRBS3 |
Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in |
Protein-protein interactors with each fusion partner protein in wild-type (BIOGRID-3.4.160) |
Hgene | Hgene's interactors | Tgene | Tgene's interactors |
- Retained PPIs in in-frame fusion. |
Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Still interaction with |
- Lost PPIs in in-frame fusion. |
Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Interaction lost with |
- Retained PPIs, but lost function due to frame-shift fusion. |
Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Interaction lost with |
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Related Drugs for XKR4-KHDRBS3 |
Drugs targeting genes involved in this fusion gene. (DrugBank Version 5.1.8 2021-05-08) |
Partner | Gene | UniProtAcc | DrugBank ID | Drug name | Drug activity | Drug type | Drug status |
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Related Diseases for XKR4-KHDRBS3 |
Diseases associated with fusion partners. (DisGeNet 4.0) |
Partner | Gene | Disease ID | Disease name | # pubmeds | Source |
Hgene | XKR4 | C0013146 | Drug abuse | 1 | CTD_human |
Hgene | XKR4 | C0013170 | Drug habituation | 1 | CTD_human |
Hgene | XKR4 | C0013222 | Drug Use Disorders | 1 | CTD_human |
Hgene | XKR4 | C0029231 | Organic Mental Disorders, Substance-Induced | 1 | CTD_human |
Hgene | XKR4 | C0038580 | Substance Dependence | 1 | CTD_human |
Hgene | XKR4 | C0038586 | Substance Use Disorders | 1 | CTD_human |
Hgene | XKR4 | C0236969 | Substance-Related Disorders | 1 | CTD_human |
Hgene | XKR4 | C0740858 | Substance abuse problem | 1 | CTD_human |
Hgene | XKR4 | C1510472 | Drug Dependence | 1 | CTD_human |
Hgene | XKR4 | C4316881 | Prescription Drug Abuse | 1 | CTD_human |