|
||||||
|
Fusion gene:BRD4_ZC3H15 (FusionGDB2 ID:HG23476TG55854) |
Fusion Gene Summary for BRD4_ZC3H15 |
 Fusion gene summary |
| Fusion gene information | Fusion gene name: BRD4_ZC3H15 | Fusion gene ID: hg23476tg55854 | Hgene | Tgene | Gene symbol | BRD4 | ZC3H15 | Gene ID | 23476 | 55854 |
| Gene name | bromodomain containing 4 | zinc finger CCCH-type containing 15 | |
| Synonyms | CAP|CDLS6|FSHRG4|HUNK1|HUNKI|MCAP | DFRP1|HT010|LEREPO4|MSTP012 | |
| Cytomap | 19p13.12 | 2q32.1 | |
| Type of gene | protein-coding | protein-coding | |
| Description | bromodomain-containing protein 4chromosome-associated proteinfemale sterile homeotic related gene 4mitotic chromosome-associated protein | zinc finger CCCH domain-containing protein 15DRG family-regulatory protein 1likely ortholog of mouse immediate early response erythropoietin 4 | |
| Modification date | 20240416 | 20240305 | |
| UniProtAcc | O60885 | . | |
| Ensembl transtripts involved in fusion gene | ENST00000360016, ENST00000263377, ENST00000371835, ENST00000602230, | ||
| Fusion gene scores | * DoF score | * DoF score (Degree of Frequency) = # partners X # break points X # disease types 45 X 81 X 27=98415 | * DoF score (Degree of Frequency) = # partners X # break points X # disease types 3 X 10 X 6=180 |
| # samples | 117 | 9 | |
| ** MAII score | ** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10) log2(117/98415*10)=-6.394297786907 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0 | ** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10) log2(9/180*10)=-1 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0 | |
| Context | PubMed: BRD4 [Title/Abstract] AND ZC3H15 [Title/Abstract] AND fusion [Title/Abstract] | ||
| Most frequent breakpoint | BRD4(15443100)-ZC3H15(187367222), # samples:1 | ||
Top |
Fusion Gene Breakpoints for BRD4_ZC3H15 |
| RNA-seq based exon junction arranged fusion gene breakpoints from 8 resources (TCGA, CCLE, cBioPortal, GenBank, ChimerDB, ChimerKB, ChildHoodFusions, and GTEx). For the expressed sample information, go to Fusion Gene Sample section. |
| Hgene | Hchr | Hbp | Tgene | Tchr | Tbp |
| BRD4 | chr19 | 15443100 | ZC3H15 | chr2 | 15443100 |
| BRD4 | chr19 | 15443101 | ZC3H15 | chr2 | 187367223 |
| BRD4 | chr19 | 15332290 | ZC3H15 | chr2 | 186502496 |
| DNA-seq based exon junction arranged fusion gene breakpoints from dbVar. For the expressed sample information, go to Fusion Gene Sample section. |
| Hgene | Hchr | Hbp | Tgene | Tchr | Tbp | SV type |
Top |
Tumorigenic MoA (Mechanism of Action) Scenarios of Fusion Genes for BRD4_ZC3H15 |
| To generate these tumorigenic scenario annotations, we implemented a deduction-first, retrieval-later computational framework. The pipeline first applies rule-guided reasoning across ten core mechanistic categories (M1–M10) derived from fusion gene biology to infer candidate mechanisms, tumorigenic scenarios, targeting points, and targeting backgrounds. To ensure empirical accountability, a governed Python workflow retrieves literature candidates via NCBI E-utilities and Europe PMC using tiered searches. Using JSON Schema-constrained LLM evidence judges (GPT-5.6 Luna and Terra), retrieved articles are evaluated for specificity and confidence without de novo PMID generation. This produces two distinct versions: a strict version restricted to high- or medium-confidence fusion-specific evidence, and an extended version incorporating broader gene-, pathway-, and contextual evidence. |
| Constitutively Active Kinases, Catalytic Domain Dysregulation, & Transmembrane Ligand Fusions | Aberrant Chimeric Transcription Factor / Fusion Transcription Factor Activity | Epigenetic Reprogramming / Histone Modifier Dysregulation | Chromatin Remodeling Dysregulation | Condensate-Driven Transcriptional Rewiring / LLP | Promoter / Enhancer Hijacking | Dominant-Negative Antagonism | Cell Cycle / Checkpoint Bypass / RNA Processing Dysregulation | Subcellular Mislocalization / Spatial Dysregulation | Nuclear Body / Sub-organellar Architecture Disruption & Differentiation Blockade |  |  |  |  |  |  |  |  |  |  |
* Strict version: Restricted to high- or medium-confidence fusion-specific evidence.
| Fusion Gene Name | Mechanism Category | Mechanism PubMed | Tumorigenic Scenarios | Tumorigenic Scenario PubMed | Targeting Points | Targeting PubMed | Mechanism Background | Mechanism Background PubMed |
| BRD4-ZC3H15 | Epigenetic Reprogramming / Histone Modifier Dysregulation M3 | BRD4 super-enhancer reader domain couples to ZC3H15 (LSm-5), linking transcriptional elongation to RNA processing and survival. | BET bromodomain inhibitors (JQ1, birabresib); CDK9 inhibitors | Hyperproliferative epithelial carcinomas |
* Extended version: Includes all strict-level fusion evidence plus broader gene-, pathway-, and low-confidence contextual evidence.
| Fusion Gene Name | Mechanism Category | Mechanism PubMed | Tumorigenic Scenarios | Tumorigenic Scenario PubMed | Targeting Points | Targeting PubMed | Mechanism Background | Mechanism Background PubMed |
| BRD4-ZC3H15 | Epigenetic Reprogramming / Histone Modifier Dysregulation | BRD4 super-enhancer reader domain couples to ZC3H15 (LSm-5), linking transcriptional elongation to RNA processing and survival. | Evidence level: Limited indirect gene evidence; Confidence: Low; PMID: 35027542; Title: ZC3H15 promotes glioblastoma progression through regulating EGFR stability.; PMID: 35064102; Title: ZC3H15 promotes gastric cancer progression by targeting the FBXW7/c-Myc pathway. | BET bromodomain inhibitors (JQ1, birabresib); CDK9 inhibitors | Hyperproliferative epithelial carcinomas | Evidence level: Limited indirect gene evidence; Confidence: Low; PMID: 35064102; Title: ZC3H15 promotes gastric cancer progression by targeting the FBXW7/c-Myc pathway. |
| Main function of each fusion partner protein. (from UniProt) |
| Hgene | Tgene |
| BRD4 | . |
| Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez |
| Partner | Gene | GO ID | GO term | PubMed ID |
| Hgene | BRD4 | GO:0032968 | positive regulation of transcription elongation by RNA polymerase II | 19103749 |
| Hgene | BRD4 | GO:0043123 | positive regulation of canonical NF-kappaB signal transduction | 19103749 |
| Hgene | BRD4 | GO:0045944 | positive regulation of transcription by RNA polymerase II | 23086925|23317504|24360279 |
| Hgene | BRD4 | GO:0050727 | regulation of inflammatory response | 19103749 |
| Tgene | ZC3H15 | GO:0043547 | positive regulation of GTPase activity | 23711155 |
Top |
Fusion Genomic Features for BRD4_ZC3H15 |
| FusionAI prediction of the potential fusion gene breakpoint based on the pre-mature RNA sequence context (+/- 5kb of individual partner genes, total 20kb length sequence) of In-frame fusion genes. FusionAI is a fusion gene breakpoint classifier based on convolutional neural network by comparing the fusion positive and negative sequence context of ~ 20K fusion gene data. From here, we can have the relative potentency of the 20K genomic sequence how individual sequnce will be likely used as the gene fusion breakpoints. |
| Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand | 1-p | p (fusion gene breakpoint) |
| BRD4 | chr19 | 15443100 | - | ZC3H15 | chr2 | 187367222 | + | 2.66e-12 | 1.00e+00 |
| FusionAI prediction of the potential fusion gene breakpoint based on the pre-mature RNA sequence context (+/- 5kb of individual partner genes, total 20kb length sequence) of 5UTR-3CSD fusion genes (N-truncated cases). |
| Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand | 1-p | p (fusion gene breakpoint) |
| BRD4 | chr19 | 15443100 | - | ZC3H15 | chr2 | 187367222 | + | 2.66e-12 | 1.00e+00 |
| FusionAI prediction of the potential fusion gene breakpoint based on the pre-mature RNA sequence context (+/- 5kb of individual partner genes, total 20kb length sequence) of 5CDS-3UTR fusion genes (C-truncated cases). |
| Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand | 1-p | p (fusion gene breakpoint) |
| Distribution of six genomic regulatory feature tracks across a ±5 kb window centered on the fusion breakpoints. We input the breakpoint sequences into AlphaGenome and obtained predicted genome tracks at single-base-pair resolution for each modality by running a single forward pass over the reference sequence. Specifically, for each breakpoint, AlphaGenome processed and returned predicted track data across diverse modalities, which were then averaged across all tracks within each output type and visualized across the ±5 kb window. The left panel shows the 5'-gene breakpoint ±5 kb area, and the right panel shows the 3'-gene breakpoint area, with tracks grouped by category: chromatin accessibility (DNase-seq, ATAC-seq), active transcription (RNA-seq, CAGE), and chromatin binding (ChIP-Histone, ChIP-TF). |
Top |
Fusion Gene ORF Annotations for BRD4_ZC3H15 |
| Open reading frame (ORF) analsis of fusion genes based on Ensembl gene isoform structure. * Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser. |
| ORF | Henst | Tenst | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand |
| 5UTR-3CDS | ENST00000360016 | ENST00000337859 | BRD4 | chr19 | 15443100 | - | ZC3H15 | chr2 | 187367222 | + |
| 5UTR-3UTR | ENST00000360016 | ENST00000468120 | BRD4 | chr19 | 15443100 | - | ZC3H15 | chr2 | 187367222 | + |
| ORFfinder Result Based On The Fusion Transcript Sequences of the In-frame Fusion Genes. |
| Henst | Tenst | Hgene | Hchr | Hbp | Tgene | Tchr | Tbp | Seq length (transcript) | Seq length (peptide) |
| ORFfinder Result Based On The Fusion Transcript Sequences of the 5UTR-3CDS Fusion Genes for N-Truncated Protein Search. |
| Henst | Tenst | Hgene | Hchr | Hbp | Tgene | Tchr | Tbp | Seq length (transcript) | Seq length (peptide) |
| ENST00000360016 | ENST00000337859 | BRD4 | chr19 | 15443100 | ZC3H15 | chr2 | 187367222 | 0 | 884 |
| ORFfinder Result Based On The Fusion Transcript Sequences of the 5CDS-3UTR Fusion Genes for C-Truncated Protein Search. |
| Henst | Tenst | Hgene | Hchr | Hbp | Tgene | Tchr | Tbp | Seq length (transcript) | Seq length (peptide) |
| DeepORF Prediction of The Coding Potential Based on The Fusion Transcript Sequence of In-frame Fusion Genes. DeepORF is a Coding Potential Classifier Based on Convolutional Neural Network by Comparing the Real Ribo-seq Data. If the No-coding Score < 0.5 and Coding Score > 0.5, Then The In-frame Fusion Transcript is Predicted as Being Likely Translated. |
| Henst | Tenst | Hgene | Hchr | Hbp | Tgene | Tchr | Tbp | No-coding score | Coding score |
| DeepORF Prediction of The Coding Potential Based on The Fusion Transcript Sequence of 5UTR-3CDS Fusion Genes (Potential N-Truncated Proteins). |
| Henst | Tenst | Hgene | Hchr | Hbp | Tgene | Tchr | Tbp | No-coding score | Coding score |
| ENST00000360016 | ENST00000337859 | BRD4 | chr19 | 15443100 | ZC3H15 | chr2 | 187367222 | 0.00e+00 | 1.51e-03 |
| DeepORF Prediction of The Coding Potential Based on The Fusion Transcript Sequence of 5CDS-3UTR Fusion Genes (Potential C-Truncated Proteins). |
| Henst | Tenst | Hgene | Hchr | Hbp | Tgene | Tchr | Tbp | No-coding score | Coding score |
Top |
Fusion Protein Retained/Non-Retained Functional Features for BRD4_ZC3H15 |
| Protein Level Annotation from FGviewer * Retention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at download page. Minus value of BPloci means that the break pointn is located before the CDS. |
 |
| - In-frame and retained protein feature among the 13 regional features (visualization across fusion protein length). |
| No matching images found for ${hg}_${tg}. |
| - In-frame and retained protein feature among the 13 regional features (texts). |
| Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Protein feature | Protein feature note |
| - In-frame and not-retained protein feature among the 13 regional features. |
| Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Protein feature | Protein feature note |
| - Retained PPIs in in-frame fusion. |
| Partner | Hgene | Hbp | Tgene | Tbp | ENST | UniProt | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Still interaction with |
| - Lost PPIs in in-frame fusion. |
| Partner | Hgene | Hbp | Tgene | Tbp | ENST | UniProt | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Interaction lost with |
Top |
Fusion Transcript Sequence for BRD4_ZC3H15 |
| In-frame Fusion Transcript Sequences. |
| N-Truncated Transcript (5UTR-3CDS) Sequences |
>BRD4_ZC3H15_ENST00000360016_ENST00000337859_15443100_187367222 length=1469nt Breakpoint=0nt ATACTATGGATAATTGGGATGAGAAAAAGCTGGAAGAAGTAGTGAACAAGAAGCACGGTGAGGCGGAAAAGAAAAAACCAAAAACTCAAATAGTGTGCAAGCATTTCCTGGAAGCTATTGAAAACAACAAGTATGGCTGGTTTTGGGTAT GCCCTGGAGGGGGTGATATTTGCATGTATCGTCATGCACTTCCTCCTGGATTTGTGTTGAAAAAAGATAAAAAGAAAGAAGAGAAAGAAGATGAAATTTCATTAGAAGATCTAATTGAGAGAGAGCGTTCTGCCCTAGGTCCAAATGTTA CCAAAATCACTCTAGAATCTTTTCTTGCCTGGAAGAAAAGGAAAAGACAAGAAAAGATTGATAAACTTGAACAAGATATGGAAAGAAGGAAAGCTGACTTCAAAGCAGGGAAAGCACTAGTGATCAGTGGTCGTGAAGTGTTTGAATTTC GTCCTGAACTGGTCAATGATGATGATGAGGAAGCAGATGATACCCGCTACACCCAGGGAACAGGTGGTGATGAGGTTGATGATTCAGTGAGTGTAAATGACATAGATTTAAGCCTGTACATCCCAAGAGATGTAGATGAAACAGGTATTA CTGTAGCCAGTCTTGAAAGATTCAGCACATATACTTCAGATAAAGATGAAAACAAATTAAGTGAAGCTTCTGGAGGTAGGGCTGAAAATGGTGAAAGAAGTGACTTGGAAGAGGACAACGAGAGGGAGGGAACGGAAAATGGAGCCATTG ATGCTGTTCCTGTTGATGAAAATCTTTTCACTGGAGAGGATTTGGATGAACTAGAAGAAGAATTAAATACACTTGATTTAGAAGAATGACACCAAACACATCGCTGAAAAAATTAAGTCAGCTCAGCACGAGTTGAAATTGACTACATTA ATTTCTTTCCACCTAGAATCAACAGGATGTTTATTTCCTATGCTGATTCTGGAGGAGTTAACCTCCTGCAAAAAAGGCATCTTGTCCCTACATCTTCTCTTCTGACTTTGGCTACATCTCATAGTAAGTTCAGAGTAGTTCATGATAAAT TGAAAATATAATGGTCATTGCAGAAAATGATTGATGTTGTAACTGTCCACCCAAGTAAGAAGTGTATCTGCCTTTCCATCTTTTGGTTTTCATTTGGGCATGTGCTATTACCAGAAACAACAAACTTATATTTAAAATACCCTTCATTTG ACACAGTTTTTAATGAGTGATTTAATTTCCTCTGTATTTGTATGTTTAGAAGACTGCCTAAAACATGAGCACTGTACTTCATAAAGGAAACTGCGTATGCAGATTCAGTATTGTGTATCTTTGGACAATTAGATGGACATTTAAAATGGA ACTTCTTTTATCTGACAGGATCAGCTACAATGCCCTGTGTTAAATTGTTTAAAAGTTTCCCTTTTCTTTTTTGCCAATAAAGTTGTAAATAAAGACCATCATACATTAAAATCCAAATA |
| C-Truncated Transcript (5CDS-3UTR) Sequences |
Top |
Fusion Protein Sequence for BRD4_ZC3H15 |
| In-frame Fusion Protein Sequences. |
| N-Truncated Protein (5UTR-3CDS) Sequences |
>BRD4_ZC3H15_ENST00000360016_ENST00000337859_15443100_187367222 length=884nt MPMEAIEKMASLCMRDPDEDEEEGTDEDDLEADDDLLAELNEVLGEEQKASETPPPVAQPKPEAPHPGLETTLQERLALYQTAIESARQAGDSAKMRRYDRGLKTLENLLASIRKGNAIDEADIPPPVAIGKGPASTPTYSPAPTQPAPR IASAPEPRVTLEGPSATAPASSPGLAKPQMPPGPCSPGPLAQLQSRQRDYKLAALHAKQQGDTTAAARHFRVAKSFDAVLEALSRGEPVDLSCLPPPPDQLPPDPPSPPSQPPTPATAPSTTEVPPPPRTLLEALEQRMERYQVAAAQAK SKGDQRKARMHERIVKQYQDAIRAHKAGRAVDVAELPVPPGFPPIQGLEATKPTQQSLVGVLETAMKLANQDEGPEDEEDEVPKKQNSPVAPTAQPKAPPSRTPQSGSAPTAKAPPKATSTRAQQQLAFLEGRKKQLLQAALRAKQKNDV EGAKMHLRQAKGLEPMLEASRNGLPVDITKVPPAPVNKDDFALVQRPGPGLSQEAARRYGELTKLIRQQHEMCLNHSNQFTQLGNITETTKFEKLAEDCKRSMDILKQAFVRGLPTPTARFEQRTFSVIKIFPDLSSNDMLLFIVKGINL PTPPGLSPGDLDVFVRFDFPYPNVEEAQKDKTSVIKNTDSPEFKEQFKLCINRSHRGFRRAIQTKGIKFEVVHKGGLFKTDRVLGTAQLKLDALEIACEVREILEVLDGRRPTGGRLEVMVRIREPLTAQQLETTTERWLVIDPVPAAVP TQVAGPKGKAPPVPAPARESGNRSARPLHSLSVLAFDQERLERKILALRQARRPVPPEVAQQYQDIMQRSQWQRAQLEQGGVGIRREYAAQLERQLQFYTEAARRLGNDGSRDAAKEALYRRNLVESELQRLRR |
| C-Truncated Protein (5CDS-3UTR) Sequences |
Top |
Personalized Fusion Protein Sequence for BRD4_ZC3H15 |
| TCGA Kinase/DNA-binding Domain Mutated Fusion Protein Sequences |
| Num | Gene Group | Domain Loci | Fusion Protein ID | Fusion Gene Name | Partner | Mutated Residue in WT Protein | Seq. Length | Mutated Residue in Fusion Protein |
| CCLE Kinase/DNA-binding Domain Mutated Fusion Protein Sequences |
| Num | Gene Group | Domain Loci | Fusion Protein ID | Fusion Gene Name | Partner | Mutated Residue in WT Protein | Seq. Length | Mutated Residue in Fusion Protein |
| TCGA All Mutated Fusion Protein Sequences |
| Fusion Protein ID | Sample ID | Mutated Partner | AAchange in WT | Seq. Length | AAchange in Fusion |
| CCLE All Mutated Fusion Protein Sequences |
| Fusion Protein ID | Sample ID | Mutated Partner | AAchange in WT | Seq. Length | AAchange in Fusion |
Top |
Fusion Gene Exprssed Samples for BRD4_ZC3H15 |
| RNA-seq based fusion gene expressed samples. |
| Source | Study | Disease | Sample | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand |
| ChimerDB | STAD | TCGA-HU-A4GH-01A | BRD4 | chr19 | 15442647 | - | ZC3H15 | chr2 | 0 | + | |
| ChimerDB | STAD | TCGA-HU-A4GH-01A | BRD4 | chr19 | 15443100 | - | ZC3H15 | chr2 | 15443100 | + | |
| WashU | STAD | TCGA-HU-A4GH-01A | BRD4 | chr19 | 15443101 | - | ZC3H15 | chr2 | 187367223 | + | |
| cBioPortal | STAD_TCGA_PAN_CAN_ATLAS_2018 | STAD | TCGA-HU-A4GH-01 | BRD4 | chr19 | 15332290 | ZC3H15 | chr2 | 186502496 |
| DNA-seq based fusion gene expressed samples. |
| Source | Study | Disease | Sample | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand | SV type |
Top |
Related Drugs for BRD4_ZC3H15 |
| PubMed Abstract Search With ['A-B' AND 'drug'], ['A::B' AND 'drug'] * For more details on the Studied, Reported, Approved Drugs targeting this fusion gene, Go to FusionPub. |
| PMID | Fusion Gene Name | Drug | Study Title |
| Drugs targeting genes involved in this fusion gene. (DrugBank Version 5.1.8 2021-05-08) |
| Partner | Gene | UniProtAcc | DrugBank ID | Drug name | Drug activity | Drug type | Drug status |
Copyright 2021-Present -The University of Texas Health Science Center at Houston (UTHealth)
Web File Viewing | Emergency Information |Campus Carry|Site Policies