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Fusion Gene Summary

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Fusion Gene ORF analysis

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Fusion Genomic Features

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Fusion Protein Features

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Fusion Gene Sequence

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Fusion Gene PPI analysis

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Related Drugs

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Related Diseases

Fusion gene:ACTR3-POLR1B (FusionGDB2 ID:HG10096TG84172)

Fusion Gene Summary for ACTR3-POLR1B

check button Fusion gene summary
Fusion gene informationFusion gene name: ACTR3-POLR1B
Fusion gene ID: hg10096tg84172
HgeneTgene
Gene symbol

ACTR3

POLR1B

Gene ID

10096

84172

Gene nameactin related protein 3RNA polymerase I subunit B
SynonymsARP3A135|RPA135|RPA2|Rpo1-2
Cytomap('ACTR3')('POLR1B')

2q14.1

2q14.1

Type of geneprotein-codingprotein-coding
Descriptionactin-related protein 3ARP3 actin related protein 3 homologactin-like protein 3DNA-directed RNA polymerase I subunit RPA2DNA-directed RNA polymerase I 135 kDa polypeptideDNA-directed RNA polymerase I 135kDa polypeptideRNA polymerase I subunit 2polymerase (RNA) I polypeptide B, 128kDapolymerase (RNA) I subunit B
Modification date2020032720200313
UniProtAcc..
Ensembl transtripts involved in fusion geneENST00000263238, ENST00000536059, 
ENST00000535589, 
Fusion gene scores* DoF score12 X 10 X 6=7203 X 7 X 3=63
# samples 134
** MAII scorelog2(13/720*10)=-2.46948528330122
possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs).
DoF>8 and MAII<0
log2(4/63*10)=-0.655351828612554
possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs).
DoF>8 and MAII<0
Context

PubMed: ACTR3 [Title/Abstract] AND POLR1B [Title/Abstract] AND fusion [Title/Abstract]

Most frequent breakpointACTR3(114647900)-POLR1B(113316870), # samples:2
Anticipated loss of major functional domain due to fusion event.ACTR3-POLR1B seems lost the major protein functional domain in Hgene partner, which is a essential gene due to the frame-shifted ORF.
ACTR3-POLR1B seems lost the major protein functional domain in Tgene partner, which is a cell metabolism gene due to the frame-shifted ORF.
ACTR3-POLR1B seems lost the major protein functional domain in Tgene partner, which is a essential gene due to the frame-shifted ORF.
* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

check button Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez
PartnerGeneGO IDGO termPubMed ID
HgeneACTR3

GO:0034314

Arp2/3 complex-mediated actin nucleation

11741539|17220302

HgeneACTR3

GO:0045944

positive regulation of transcription by RNA polymerase II

17220302



check button Fusion gene information from two resources (ChiTars 5.0 and ChimerDB 4.0)
* All genome coordinats were lifted-over on hg19.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.
SourceDiseaseSampleHgeneHchrHbpHstrandTgeneTchrTbpTstrand
ChimerDB4OVTCGA-61-2111-01AACTR3chr2

114647900

+POLR1Bchr2

113315487

+
ChimerDB4OVTCGA-61-2111-01AACTR3chr2

114647900

+POLR1Bchr2

113316870

+
ChimerDB4OVTCGA-61-2111ACTR3chr2

114647900

+POLR1Bchr2

113315486

+
ChimerDB4OVTCGA-61-2111ACTR3chr2

114647900

+POLR1Bchr2

113316869

+


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Fusion Gene ORF analysis for ACTR3-POLR1B

check button Open reading frame (ORF) analsis of fusion genes based on Ensembl gene isoform structure.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.
ORFHenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrand
5CDS-3UTRENST00000263238ENST00000498054ACTR3chr2

114647900

+POLR1Bchr2

113315487

+
5CDS-3UTRENST00000263238ENST00000498054ACTR3chr2

114647900

+POLR1Bchr2

113316870

+
5CDS-3UTRENST00000263238ENST00000498054ACTR3chr2

114647900

+POLR1Bchr2

113315486

+
5CDS-3UTRENST00000263238ENST00000498054ACTR3chr2

114647900

+POLR1Bchr2

113316869

+
5CDS-intronENST00000263238ENST00000409894ACTR3chr2

114647900

+POLR1Bchr2

113315487

+
5CDS-intronENST00000263238ENST00000409894ACTR3chr2

114647900

+POLR1Bchr2

113316870

+
5CDS-intronENST00000263238ENST00000409894ACTR3chr2

114647900

+POLR1Bchr2

113315486

+
5CDS-intronENST00000263238ENST00000409894ACTR3chr2

114647900

+POLR1Bchr2

113316869

+
5CDS-intronENST00000263238ENST00000417433ACTR3chr2

114647900

+POLR1Bchr2

113315487

+
5CDS-intronENST00000263238ENST00000417433ACTR3chr2

114647900

+POLR1Bchr2

113316870

+
5CDS-intronENST00000263238ENST00000417433ACTR3chr2

114647900

+POLR1Bchr2

113315486

+
5CDS-intronENST00000263238ENST00000417433ACTR3chr2

114647900

+POLR1Bchr2

113316869

+
5CDS-intronENST00000263238ENST00000537335ACTR3chr2

114647900

+POLR1Bchr2

113315487

+
5CDS-intronENST00000263238ENST00000537335ACTR3chr2

114647900

+POLR1Bchr2

113316870

+
5CDS-intronENST00000263238ENST00000537335ACTR3chr2

114647900

+POLR1Bchr2

113315486

+
5CDS-intronENST00000263238ENST00000537335ACTR3chr2

114647900

+POLR1Bchr2

113316869

+
5UTR-3CDSENST00000536059ENST00000263331ACTR3chr2

114647900

+POLR1Bchr2

113315487

+
5UTR-3CDSENST00000536059ENST00000263331ACTR3chr2

114647900

+POLR1Bchr2

113316870

+
5UTR-3CDSENST00000536059ENST00000263331ACTR3chr2

114647900

+POLR1Bchr2

113315486

+
5UTR-3CDSENST00000536059ENST00000263331ACTR3chr2

114647900

+POLR1Bchr2

113316869

+
5UTR-3CDSENST00000536059ENST00000541869ACTR3chr2

114647900

+POLR1Bchr2

113315487

+
5UTR-3CDSENST00000536059ENST00000541869ACTR3chr2

114647900

+POLR1Bchr2

113316870

+
5UTR-3CDSENST00000536059ENST00000541869ACTR3chr2

114647900

+POLR1Bchr2

113315486

+
5UTR-3CDSENST00000536059ENST00000541869ACTR3chr2

114647900

+POLR1Bchr2

113316869

+
5UTR-3UTRENST00000536059ENST00000498054ACTR3chr2

114647900

+POLR1Bchr2

113315487

+
5UTR-3UTRENST00000536059ENST00000498054ACTR3chr2

114647900

+POLR1Bchr2

113316870

+
5UTR-3UTRENST00000536059ENST00000498054ACTR3chr2

114647900

+POLR1Bchr2

113315486

+
5UTR-3UTRENST00000536059ENST00000498054ACTR3chr2

114647900

+POLR1Bchr2

113316869

+
5UTR-intronENST00000536059ENST00000409894ACTR3chr2

114647900

+POLR1Bchr2

113315487

+
5UTR-intronENST00000536059ENST00000409894ACTR3chr2

114647900

+POLR1Bchr2

113316870

+
5UTR-intronENST00000536059ENST00000409894ACTR3chr2

114647900

+POLR1Bchr2

113315486

+
5UTR-intronENST00000536059ENST00000409894ACTR3chr2

114647900

+POLR1Bchr2

113316869

+
5UTR-intronENST00000536059ENST00000417433ACTR3chr2

114647900

+POLR1Bchr2

113315487

+
5UTR-intronENST00000536059ENST00000417433ACTR3chr2

114647900

+POLR1Bchr2

113316870

+
5UTR-intronENST00000536059ENST00000417433ACTR3chr2

114647900

+POLR1Bchr2

113315486

+
5UTR-intronENST00000536059ENST00000417433ACTR3chr2

114647900

+POLR1Bchr2

113316869

+
5UTR-intronENST00000536059ENST00000537335ACTR3chr2

114647900

+POLR1Bchr2

113315487

+
5UTR-intronENST00000536059ENST00000537335ACTR3chr2

114647900

+POLR1Bchr2

113316870

+
5UTR-intronENST00000536059ENST00000537335ACTR3chr2

114647900

+POLR1Bchr2

113315486

+
5UTR-intronENST00000536059ENST00000537335ACTR3chr2

114647900

+POLR1Bchr2

113316869

+
Frame-shiftENST00000263238ENST00000263331ACTR3chr2

114647900

+POLR1Bchr2

113315487

+
Frame-shiftENST00000263238ENST00000263331ACTR3chr2

114647900

+POLR1Bchr2

113316870

+
Frame-shiftENST00000263238ENST00000263331ACTR3chr2

114647900

+POLR1Bchr2

113315486

+
Frame-shiftENST00000263238ENST00000263331ACTR3chr2

114647900

+POLR1Bchr2

113316869

+
Frame-shiftENST00000263238ENST00000541869ACTR3chr2

114647900

+POLR1Bchr2

113315487

+
Frame-shiftENST00000263238ENST00000541869ACTR3chr2

114647900

+POLR1Bchr2

113316870

+
Frame-shiftENST00000263238ENST00000541869ACTR3chr2

114647900

+POLR1Bchr2

113315486

+
Frame-shiftENST00000263238ENST00000541869ACTR3chr2

114647900

+POLR1Bchr2

113316869

+
intron-3CDSENST00000535589ENST00000263331ACTR3chr2

114647900

+POLR1Bchr2

113315487

+
intron-3CDSENST00000535589ENST00000263331ACTR3chr2

114647900

+POLR1Bchr2

113316870

+
intron-3CDSENST00000535589ENST00000263331ACTR3chr2

114647900

+POLR1Bchr2

113315486

+
intron-3CDSENST00000535589ENST00000263331ACTR3chr2

114647900

+POLR1Bchr2

113316869

+
intron-3CDSENST00000535589ENST00000541869ACTR3chr2

114647900

+POLR1Bchr2

113315487

+
intron-3CDSENST00000535589ENST00000541869ACTR3chr2

114647900

+POLR1Bchr2

113316870

+
intron-3CDSENST00000535589ENST00000541869ACTR3chr2

114647900

+POLR1Bchr2

113315486

+
intron-3CDSENST00000535589ENST00000541869ACTR3chr2

114647900

+POLR1Bchr2

113316869

+
intron-3UTRENST00000535589ENST00000498054ACTR3chr2

114647900

+POLR1Bchr2

113315487

+
intron-3UTRENST00000535589ENST00000498054ACTR3chr2

114647900

+POLR1Bchr2

113316870

+
intron-3UTRENST00000535589ENST00000498054ACTR3chr2

114647900

+POLR1Bchr2

113315486

+
intron-3UTRENST00000535589ENST00000498054ACTR3chr2

114647900

+POLR1Bchr2

113316869

+
intron-intronENST00000535589ENST00000409894ACTR3chr2

114647900

+POLR1Bchr2

113315487

+
intron-intronENST00000535589ENST00000409894ACTR3chr2

114647900

+POLR1Bchr2

113316870

+
intron-intronENST00000535589ENST00000409894ACTR3chr2

114647900

+POLR1Bchr2

113315486

+
intron-intronENST00000535589ENST00000409894ACTR3chr2

114647900

+POLR1Bchr2

113316869

+
intron-intronENST00000535589ENST00000417433ACTR3chr2

114647900

+POLR1Bchr2

113315487

+
intron-intronENST00000535589ENST00000417433ACTR3chr2

114647900

+POLR1Bchr2

113316870

+
intron-intronENST00000535589ENST00000417433ACTR3chr2

114647900

+POLR1Bchr2

113315486

+
intron-intronENST00000535589ENST00000417433ACTR3chr2

114647900

+POLR1Bchr2

113316869

+
intron-intronENST00000535589ENST00000537335ACTR3chr2

114647900

+POLR1Bchr2

113315487

+
intron-intronENST00000535589ENST00000537335ACTR3chr2

114647900

+POLR1Bchr2

113316870

+
intron-intronENST00000535589ENST00000537335ACTR3chr2

114647900

+POLR1Bchr2

113315486

+
intron-intronENST00000535589ENST00000537335ACTR3chr2

114647900

+POLR1Bchr2

113316869

+

check buttonORFfinder result based on the fusion transcript sequence of in-frame fusion genes.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandSeq length
(transcript)
BP loci
(transcript)
Predicted start
(transcript)
Predicted stop
(transcript)
Seq length
(amino acids)

check buttonDeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandNo-coding scoreCoding score

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Fusion Genomic Features for ACTR3-POLR1B


check buttonFusionAI prediction of the potential fusion gene breakpoint based on the pre-mature RNA sequence context (+/- 5kb of individual partner genes, total 20kb length sequence). FusionAI is a fusion gene breakpoint classifier based on convolutional neural network by comparing the fusion positive and negative sequence context of ~ 20K fusion gene data. From here, we can have the relative potentency of the 20K genomic sequence how individual sequnce will be likely used as the gene fusion breakpoints.
HgeneHchrHbpHstrandTgeneTchrTbpTstrand1-pp (fusion gene breakpoint)
ACTR3chr2114647900+POLR1Bchr2113316869+4.06E-111
ACTR3chr2114647900+POLR1Bchr2113315486+7.56E-101
ACTR3chr2114647900+POLR1Bchr2113316869+4.06E-111
ACTR3chr2114647900+POLR1Bchr2113315486+7.56E-101
ACTR3chr2114647900+POLR1Bchr2113316869+4.06E-111
ACTR3chr2114647900+POLR1Bchr2113315486+7.56E-101
ACTR3chr2114647900+POLR1Bchr2113316869+4.06E-111
ACTR3chr2114647900+POLR1Bchr2113315486+7.56E-101


check buttonDistribution of 44 human genomic features loci across 20kb length fusion breakpoint regions that are ovelapped with the top 1% feature importance score regions. More details are in help page.
genomic feature of top 1%

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Fusion Protein Features for ACTR3-POLR1B


check button Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/:114647900/:113316870)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
4. Go down and press 'Search' tab twice.
4. Go down to have the hyperlink of the search result.
5. Click the hyperlink.
6. See the FGviewer result for your fusion gene.
FGviewer

check buttonMain function of each fusion partner protein. (from UniProt)
HgeneTgene
..
FUNCTION: Transcriptional activator which is required for calcium-dependent dendritic growth and branching in cortical neurons. Recruits CREB-binding protein (CREBBP) to nuclear bodies. Component of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating a calcium-dependent release of a repressor complex and a recruitment of an activator complex. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex. At the same time, there is increased recruitment of CREBBP to the promoter by a CREST-dependent mechanism, which leads to transcriptional activation. The CREST-BRG1 complex also binds to the NR2B promoter, and activity-dependent induction of NR2B expression involves a release of HDAC1 and recruitment of CREBBP (By similarity). {ECO:0000250}.FUNCTION: Transcriptional activator which is required for calcium-dependent dendritic growth and branching in cortical neurons. Recruits CREB-binding protein (CREBBP) to nuclear bodies. Component of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating a calcium-dependent release of a repressor complex and a recruitment of an activator complex. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex. At the same time, there is increased recruitment of CREBBP to the promoter by a CREST-dependent mechanism, which leads to transcriptional activation. The CREST-BRG1 complex also binds to the NR2B promoter, and activity-dependent induction of NR2B expression involves a release of HDAC1 and recruitment of CREBBP (By similarity). {ECO:0000250}.

check buttonRetention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at

download page


* Minus value of BPloci means that the break pointn is located before the CDS.
- In-frame and retained protein feature among the 13 regional features.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note

- In-frame and not-retained protein feature among the 13 regional features.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note


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Fusion Gene Sequence for ACTR3-POLR1B


check button For in-frame fusion transcripts, we provide the fusion transcript sequences and fusion amino acid sequences. To have fusion amino acid sequence, we ran ORFfinder and chose the longest ORF among the all predicted ones.

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Fusion Gene PPI Analysis for ACTR3-POLR1B


check button Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in

ChiPPI page.


check button Protein-protein interactors with each fusion partner protein in wild-type (BIOGRID-3.4.160)
HgeneHgene's interactorsTgeneTgene's interactors


check button - Retained PPIs in in-frame fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenStill interaction with


check button - Lost PPIs in in-frame fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenInteraction lost with


check button - Retained PPIs, but lost function due to frame-shift fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenInteraction lost with


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Related Drugs for ACTR3-POLR1B


check button Drugs targeting genes involved in this fusion gene.
(DrugBank Version 5.1.8 2021-05-08)
PartnerGeneUniProtAccDrugBank IDDrug nameDrug activityDrug typeDrug status

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Related Diseases for ACTR3-POLR1B


check button Diseases associated with fusion partners.
(DisGeNet 4.0)
PartnerGeneDisease IDDisease name# pubmedsSource
HgeneACTR3C0019193Hepatitis, Toxic1CTD_human
HgeneACTR3C0860207Drug-Induced Liver Disease1CTD_human
HgeneACTR3C1262760Hepatitis, Drug-Induced1CTD_human
HgeneACTR3C3658290Drug-Induced Acute Liver Injury1CTD_human
HgeneACTR3C4277682Chemical and Drug Induced Liver Injury1CTD_human
HgeneACTR3C4279912Chemically-Induced Liver Toxicity1CTD_human