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Center for Computational Systems Medicine
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Fusion Gene Summary

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Fusion Gene ORF analysis

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Fusion Genomic Features

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Fusion Protein Features

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Fusion Gene Sequence

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Fusion Gene PPI analysis

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Related Drugs

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Related Diseases

Fusion gene:ARIH2-TRIO (FusionGDB2 ID:HG10425TG7204)

Fusion Gene Summary for ARIH2-TRIO

check button Fusion gene summary
Fusion gene informationFusion gene name: ARIH2-TRIO
Fusion gene ID: hg10425tg7204
HgeneTgene
Gene symbol

ARIH2

TRIO

Gene ID

10425

7204

Gene nameariadne RBR E3 ubiquitin protein ligase 2trio Rho guanine nucleotide exchange factor
SynonymsARI2|TRIAD1ARHGEF23|MEBAS|MRD44|tgat
Cytomap('ARIH2')('TRIO')

3p21.31

5p15.2

Type of geneprotein-codingprotein-coding
DescriptionE3 ubiquitin-protein ligase ARIH2RING-type E3 ubiquitin transferase ARIH2all-trans retinoic acid inducible RING fingerariadne homolog 2protein ariadne-2 homologtriple functional domain proteinPTPRF-interacting proteintriple functional domain (PTPRF interacting)
Modification date2020031320200313
UniProtAcc..
Ensembl transtripts involved in fusion geneENST00000490095, ENST00000356401, 
ENST00000449376, 
Fusion gene scores* DoF score10 X 9 X 7=6308 X 8 X 4=256
# samples 128
** MAII scorelog2(12/630*10)=-2.39231742277876
possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs).
DoF>8 and MAII<0
log2(8/256*10)=-1.67807190511264
possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs).
DoF>8 and MAII<0
Context

PubMed: ARIH2 [Title/Abstract] AND TRIO [Title/Abstract] AND fusion [Title/Abstract]

Most frequent breakpointARIH2(48956431)-TRIO(14286980), # samples:2
Anticipated loss of major functional domain due to fusion event.
* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

check button Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez
PartnerGeneGO IDGO termPubMed ID
HgeneARIH2

GO:0000209

protein polyubiquitination

16118314|19340006

HgeneARIH2

GO:0006511

ubiquitin-dependent protein catabolic process

16118314

HgeneARIH2

GO:0016567

protein ubiquitination

24076655

HgeneARIH2

GO:0048588

developmental cell growth

19340006

HgeneARIH2

GO:0070534

protein K63-linked ubiquitination

19340006

HgeneARIH2

GO:0070936

protein K48-linked ubiquitination

16118314|19340006

HgeneARIH2

GO:0071425

hematopoietic stem cell proliferation

16118314|19340006



check button Fusion gene information
* All genome coordinats were lifted-over on hg19.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.
SourceDiseaseSampleHgeneHchrHbpHstrandTgeneTchrTbpTstrand
ChimerDB4HNSCTCGA-BA-5153-01AARIH2chr3

48956431

-TRIOchr5

14286980

+
ChimerDB4HNSCTCGA-BA-5153-01AARIH2chr3

48956431

+TRIOchr5

14286980

+


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Fusion Gene ORF analysis for ARIH2-TRIO

check button Open reading frame (ORF) analsis of fusion genes based on Ensembl gene isoform structure.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.
ORFHenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrand
3UTR-3CDSENST00000490095ENST00000344204ARIH2chr3

48956431

+TRIOchr5

14286980

+
3UTR-3CDSENST00000490095ENST00000509967ARIH2chr3

48956431

+TRIOchr5

14286980

+
3UTR-3CDSENST00000490095ENST00000537187ARIH2chr3

48956431

+TRIOchr5

14286980

+
3UTR-intronENST00000490095ENST00000344135ARIH2chr3

48956431

+TRIOchr5

14286980

+
3UTR-intronENST00000490095ENST00000515710ARIH2chr3

48956431

+TRIOchr5

14286980

+
5UTR-3CDSENST00000356401ENST00000344204ARIH2chr3

48956431

+TRIOchr5

14286980

+
5UTR-3CDSENST00000356401ENST00000509967ARIH2chr3

48956431

+TRIOchr5

14286980

+
5UTR-3CDSENST00000356401ENST00000537187ARIH2chr3

48956431

+TRIOchr5

14286980

+
5UTR-3CDSENST00000449376ENST00000344204ARIH2chr3

48956431

+TRIOchr5

14286980

+
5UTR-3CDSENST00000449376ENST00000509967ARIH2chr3

48956431

+TRIOchr5

14286980

+
5UTR-3CDSENST00000449376ENST00000537187ARIH2chr3

48956431

+TRIOchr5

14286980

+
5UTR-intronENST00000356401ENST00000344135ARIH2chr3

48956431

+TRIOchr5

14286980

+
5UTR-intronENST00000356401ENST00000515710ARIH2chr3

48956431

+TRIOchr5

14286980

+
5UTR-intronENST00000449376ENST00000344135ARIH2chr3

48956431

+TRIOchr5

14286980

+
5UTR-intronENST00000449376ENST00000515710ARIH2chr3

48956431

+TRIOchr5

14286980

+

check buttonORFfinder result based on the fusion transcript sequence of in-frame fusion genes.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandSeq length
(transcript)
BP loci
(transcript)
Predicted start
(transcript)
Predicted stop
(transcript)
Seq length
(amino acids)

check buttonDeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandNo-coding scoreCoding score

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Fusion Genomic Features for ARIH2-TRIO


check buttonFusionAI prediction of the potential fusion gene breakpoint based on the pre-mature RNA sequence context (+/- 5kb of individual partner genes, total 20kb length sequence). FusionAI is a fusion gene breakpoint classifier based on convolutional neural network by comparing the fusion positive and negative sequence context of ~ 20K fusion gene data. From here, we can have the relative potentency of the 20K genomic sequence how individual sequnce will be likely used as the gene fusion breakpoints.
HgeneHchrHbpHstrandTgeneTchrTbpTstrand1-pp (fusion gene breakpoint)
ARIH2chr348956431+TRIOchr514286979+8.84E-121
ARIH2chr348956431+TRIOchr514286979+8.84E-121


check buttonDistribution of 44 human genomic features loci across 20kb length fusion breakpoint regions that are ovelapped with the top 1% feature importance score regions. More details are in help page.
genomic feature of top 1%

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Fusion Protein Features for ARIH2-TRIO


check button Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/:48956431/:14286980)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
4. Go down and press 'Search' tab twice.
4. Go down to have the hyperlink of the search result.
5. Click the hyperlink.
6. See the FGviewer result for your fusion gene.
FGviewer

check buttonMain function of each fusion partner protein. (from UniProt)
HgeneTgene
..
FUNCTION: Transcriptional activator which is required for calcium-dependent dendritic growth and branching in cortical neurons. Recruits CREB-binding protein (CREBBP) to nuclear bodies. Component of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating a calcium-dependent release of a repressor complex and a recruitment of an activator complex. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex. At the same time, there is increased recruitment of CREBBP to the promoter by a CREST-dependent mechanism, which leads to transcriptional activation. The CREST-BRG1 complex also binds to the NR2B promoter, and activity-dependent induction of NR2B expression involves a release of HDAC1 and recruitment of CREBBP (By similarity). {ECO:0000250}.FUNCTION: Transcriptional activator which is required for calcium-dependent dendritic growth and branching in cortical neurons. Recruits CREB-binding protein (CREBBP) to nuclear bodies. Component of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating a calcium-dependent release of a repressor complex and a recruitment of an activator complex. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex. At the same time, there is increased recruitment of CREBBP to the promoter by a CREST-dependent mechanism, which leads to transcriptional activation. The CREST-BRG1 complex also binds to the NR2B promoter, and activity-dependent induction of NR2B expression involves a release of HDAC1 and recruitment of CREBBP (By similarity). {ECO:0000250}.

check buttonRetention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at

download page


* Minus value of BPloci means that the break pointn is located before the CDS.
- In-frame and retained protein feature among the 13 regional features.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note

- In-frame and not-retained protein feature among the 13 regional features.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note


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Fusion Gene Sequence for ARIH2-TRIO


check button For in-frame fusion transcripts, we provide the fusion transcript sequences and fusion amino acid sequences. To have fusion amino acid sequence, we ran ORFfinder and chose the longest ORF among the all predicted ones.

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Fusion Gene PPI Analysis for ARIH2-TRIO


check button Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in

ChiPPI page.


check button Protein-protein interactors with each fusion partner protein in wild-type (BIOGRID-3.4.160)
HgeneHgene's interactorsTgeneTgene's interactors


check button - Retained PPIs in in-frame fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenStill interaction with


check button - Lost PPIs in in-frame fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenInteraction lost with


check button - Retained PPIs, but lost function due to frame-shift fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenInteraction lost with


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Related Drugs for ARIH2-TRIO


check button Drugs targeting genes involved in this fusion gene.
(DrugBank Version 5.1.8 2021-05-08)
PartnerGeneUniProtAccDrugBank IDDrug nameDrug activityDrug typeDrug status

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Related Diseases for ARIH2-TRIO


check button Diseases associated with fusion partners.
(DisGeNet 4.0)
PartnerGeneDisease IDDisease name# pubmedsSource
TgeneC4310740MENTAL RETARDATION, AUTOSOMAL DOMINANT 442CTD_human;ORPHANET;UNIPROT
TgeneC0023467Leukemia, Myelocytic, Acute1CTD_human
TgeneC0023903Liver neoplasms1CTD_human
TgeneC0026998Acute Myeloid Leukemia, M11CTD_human
TgeneC0032927Precancerous Conditions1CTD_human
TgeneC0282313Condition, Preneoplastic1CTD_human
TgeneC0345904Malignant neoplasm of liver1CTD_human
TgeneC1535926Neurodevelopmental Disorders1CTD_human
TgeneC1879321Acute Myeloid Leukemia (AML-M2)1CTD_human