Fusion Gene Studies
in Kim Lab

FusionBase FusionGDB FusionGDB2 FusionPDB FusionNeoAntigen FusionAI FusionNW FGviewer Publication Contact
FusionGDB Logo

Home

Download

Statistics

Examples

Help

Contact

Center for Computational Systems Medicine
leaf

Fusion Gene Summary

leaf

Fusion Gene ORF analysis

leaf

Fusion Genomic Features

leaf

Fusion Protein Features

leaf

Fusion Gene Sequence

leaf

Fusion Gene PPI analysis

leaf

Related Drugs

leaf

Related Diseases

Fusion gene:AAMP-MARCH4 (FusionGDB2 ID:HG14TG57574)

Fusion Gene Summary for AAMP-MARCH4

check button Fusion gene summary
Fusion gene informationFusion gene name: AAMP-MARCH4
Fusion gene ID: hg14tg57574
HgeneTgene
Gene symbol

AAMP

MARCH4

Gene ID

14

57574

Gene nameangio associated migratory cell proteinmembrane associated ring-CH-type finger 4
Synonyms-MARCH-IV|MARCH4|RNF174
Cytomap('AAMP')('MARCH4')

2q35

2q35

Type of geneprotein-codingprotein-coding
Descriptionangio-associated migratory cell proteinE3 ubiquitin-protein ligase MARCH4RING finger protein 174RING-type E3 ubiquitin transferase MARCH4membrane associated ring finger 4membrane-associated RING finger protein 4membrane-associated RING-CH protein IVmembrane-associated ring finger (C3HC4)
Modification date2020032020200313
UniProtAcc

Q13685

.
Ensembl transtripts involved in fusion geneENST00000248450, ENST00000444053, 
ENST00000420660, 
Fusion gene scores* DoF score2 X 1 X 2=44 X 3 X 4=48
# samples 24
** MAII scorelog2(2/4*10)=2.32192809488736log2(4/48*10)=-0.263034405833794
possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs).
DoF>8 and MAII<0
Context

PubMed: AAMP [Title/Abstract] AND MARCH4 [Title/Abstract] AND fusion [Title/Abstract]

Most frequent breakpointAAMP(219134105)-MARCH4(217124402), # samples:3
Anticipated loss of major functional domain due to fusion event.AAMP-MARCH4 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF.
AAMP-MARCH4 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF.
AAMP-MARCH4 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF.
AAMP-MARCH4 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF.
AAMP-MARCH4 seems lost the major protein functional domain in Hgene partner, which is a essential gene due to the frame-shifted ORF.
* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

check button Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez
PartnerGeneGO IDGO termPubMed ID

check buttonFusion gene breakpoints across AAMP (5'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.
all structure
check buttonFusion gene breakpoints across MARCH4 (3'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.
all structure

check button Fusion gene information
* All genome coordinats were lifted-over on hg19.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.
SourceDiseaseSampleHgeneHchrHbpHstrandTgeneTchrTbpTstrand
ChimerDB4LUSCTCGA-21-1083-01AAAMPchr2

219134105

-MARCH4chr2

217124402

-
ChimerDB4LUSCTCGA-21-1083AAMPchr2

219134105

-MARCH4chr2

217124402

-


Top

Fusion Gene ORF analysis for AAMP-MARCH4

check button Open reading frame (ORF) analsis of fusion genes based on Ensembl gene isoform structure.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.
ORFHenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrand
Frame-shiftENST00000248450ENST00000273067AAMPchr2

219134105

-MARCH4chr2

217124402

-
Frame-shiftENST00000444053ENST00000273067AAMPchr2

219134105

-MARCH4chr2

217124402

-
In-frameENST00000420660ENST00000273067AAMPchr2

219134105

-MARCH4chr2

217124402

-

check buttonORFfinder result based on the fusion transcript sequence of in-frame fusion genes.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandSeq length
(transcript)
BP loci
(transcript)
Predicted start
(transcript)
Predicted stop
(transcript)
Seq length
(amino acids)
ENST00000420660AAMPchr2219134105-ENST00000273067MARCH4chr2217124402-2174359142726194

check buttonDeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandNo-coding scoreCoding score
ENST00000420660ENST00000273067AAMPchr2219134105-MARCH4chr2217124402-0.0055157940.99448425

Top

Fusion Genomic Features for AAMP-MARCH4


check buttonFusionAI prediction of the potential fusion gene breakpoint based on the pre-mature RNA sequence context (+/- 5kb of individual partner genes, total 20kb length sequence). FusionAI is a fusion gene breakpoint classifier based on convolutional neural network by comparing the fusion positive and negative sequence context of ~ 20K fusion gene data. From here, we can have the relative potentency of the 20K genomic sequence how individual sequnce will be likely used as the gene fusion breakpoints.
HgeneHchrHbpHstrandTgeneTchrTbpTstrand1-pp (fusion gene breakpoint)

check buttonDistribution of 44 human genomic features loci across 20kb length fusion breakpoint regions. We integrated a total of 44 different types of human genomic feature loci information across five big categories including virus integration sites, repeats, structural variants, chromatin states, and gene expression regulation. More details are in help page.
genomic feature

Top

Fusion Protein Features for AAMP-MARCH4


check button Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chr2:219134105/chr2:217124402)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
4. Go down and press 'Search' tab twice.
4. Go down to have the hyperlink of the search result.
5. Click the hyperlink.
6. See the FGviewer result for your fusion gene.
FGviewer

check buttonMain function of each fusion partner protein. (from UniProt)
HgeneTgene
AAMP

Q13685

.
FUNCTION: Plays a role in angiogenesis and cell migration. In smooth muscle cell migration, may act through the RhoA pathway. {ECO:0000269|PubMed:10329261, ECO:0000269|PubMed:18634987}.FUNCTION: Transcriptional activator which is required for calcium-dependent dendritic growth and branching in cortical neurons. Recruits CREB-binding protein (CREBBP) to nuclear bodies. Component of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating a calcium-dependent release of a repressor complex and a recruitment of an activator complex. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex. At the same time, there is increased recruitment of CREBBP to the promoter by a CREST-dependent mechanism, which leads to transcriptional activation. The CREST-BRG1 complex also binds to the NR2B promoter, and activity-dependent induction of NR2B expression involves a release of HDAC1 and recruitment of CREBBP (By similarity). {ECO:0000250}.

check buttonRetention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at

download page


* Minus value of BPloci means that the break pointn is located before the CDS.
- In-frame and retained protein feature among the 13 regional features.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note
HgeneAAMPchr2:219134105chr2:217124402ENST00000248450-21153_5991435.0Compositional biasNote=Poly-Glu

- In-frame and not-retained protein feature among the 13 regional features.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note
HgeneAAMPchr2:219134105chr2:217124402ENST00000248450-211132_17191435.0RepeatNote=WD 2
HgeneAAMPchr2:219134105chr2:217124402ENST00000248450-211173_21291435.0RepeatNote=WD 3
HgeneAAMPchr2:219134105chr2:217124402ENST00000248450-211214_25491435.0RepeatNote=WD 4
HgeneAAMPchr2:219134105chr2:217124402ENST00000248450-211258_29991435.0RepeatNote=WD 5
HgeneAAMPchr2:219134105chr2:217124402ENST00000248450-211315_35491435.0RepeatNote=WD 6
HgeneAAMPchr2:219134105chr2:217124402ENST00000248450-211356_39591435.0RepeatNote=WD 7
HgeneAAMPchr2:219134105chr2:217124402ENST00000248450-211398_43391435.0RepeatNote=WD 8
HgeneAAMPchr2:219134105chr2:217124402ENST00000248450-21189_12991435.0RepeatNote=WD 1
TgeneMARCH4chr2:219134105chr2:217124402ENST000002730672457_127288411.0Compositional biasNote=Pro-rich
TgeneMARCH4chr2:219134105chr2:217124402ENST0000027306724238_258288411.0TransmembraneHelical
TgeneMARCH4chr2:219134105chr2:217124402ENST0000027306724272_292288411.0TransmembraneHelical
TgeneMARCH4chr2:219134105chr2:217124402ENST0000027306724155_215288411.0Zinc fingerRING-CH-type


Top

Fusion Gene Sequence for AAMP-MARCH4


check button For in-frame fusion transcripts, we provide the fusion transcript sequences and fusion amino acid sequences. To have fusion amino acid sequence, we ran ORFfinder and chose the longest ORF among the all predicted ones.
>78_78_1_AAMP-MARCH4_AAMP_chr2_219134105_ENST00000420660_MARCH4_chr2_217124402_ENST00000273067_length(transcript)=2174nt_BP=359nt
TCCCCTTGGCCCTCCCTTACCGCACGGTCTCTGGCCAGCCGTGGCCTCCCGTCCTGTGTCCCGCGCGCCCCCGCGGACCCCGGTGTGTCT
GACTCCACCTTCCCTTCTCGCCACATGACCCGCCCCAGCCTCAGGCCTCCTCATGCTTCTCTCCGTACCTCTTACCGCCTCCCGAGCCCC
ATCTTCTAACCCTCTCCCCAAAGCAGATGACCTGGCCCAGGAGATGGAAGATGTGGACTTTGAGGAAGAAGAGGAGGAAGAGGGCAACGA
AGAGGGCTGGGTTCTAGAACCCCAGGAAGGGGTGGTCGGCAGCATGGAGGGCCCCGACGATAGCGAGGTCACCTTTGCATTGCACTCAGG
TCTCATCATCCATGAAGGACCCTCGGTGTACCGCATCTTTAAACGGTGGCAGGCTGTCAACCAGCAGTGGAAAGTGCTGAACTATGACAA
GACAAAAGACCTGGAGGATCAAAAGGCAGGAGGCAGGACCAACCCCCGGACCTCCTCATCCACCCAGGCCAATATCCCCTCCTCGGAAGA
GGAGACCGCAGGCACCCCTGCCCCTGAGCAGGGCCCTGCCCAGGCTGCCGGCCACCCCTCAGGCCCTCTGTCCCATCACCACTGTGCTTA
TACCATCCTGCACATCCTGAGTCACTTGAGACCTCATGAACAGCGAAGTCCCCCAGGCAGCAGCCGAGAGCTGGTCATGAGAGTCACGAC
AGTGTGAGAGCAGAGGCCCGGAAGGAAGGCCATGACCACCACTGAGGGCCCGGAGCAGGGTGGGGAGGTGCAGTGGCACCCCCAGAGCCA
ACAGAGGGAGCAGGCAGAGGGTGGGGGACCTGGCGGGAGCCCTGGGGTAGTGTCAGAGCGGGAGTGAGGCTGGTGCAGGAGCAGTTCTGC
TATTTCCAATCAGTCAATGCCACTCTCCACAACAACAATGAAAACCAACACCAACTCAACAACAAAGTGCAATACAGGCTGAACCTGGCC
CAACAGAAAAACCCTGCCCCAATGCACCTGCAGGCAAGGTACCCGAAGAAGCAGAGGCTGAGGGCAGGCAAAGCCTGTGTGACTGTGGCA
GTGCCGGAGGCCAAGGGGGCCAAGAGGAAAAGCATCTGTGGTCTGCTCTGCTCTCACCCTGTTTGGTTTTGTTTCTCCTGGGGCTGTGTT
CTGCAGGCAGCCAGAAAAGGAGGAGGCACGGGTGAGCTGGCAGGGACACACTGCCTTTGGGGCTCCTGGGCTCATTTGGATGGGCAAGAT
TCGCTGACAAATGGCTGTGGGGATGGTGGGGTGGATGGTCAGGGAGGGATGCTCAGGGAGGGATATGCTGGTGTGAGCAGCCAGAGGGAG
AGTGTGTCTCCTTCCTGAAGGAACTTCCAAATGGAACTCCCGATTTCAGGTGGGCTAAAAGAGGGCTTAGGTTTGGAAAAGGGTGTCCTT
CTGTGCCCTTGTTAATTTATTTTATAGTGATTTGGTTCAAAGATGTTTACAGGACACACACACACACACACACACACACACACACACACA
CCCCTAGAGAAAAGTACAGATTTCCAGTGGATATTTCAAGCACAGTTCTGCTGCTGTGGCTTCAGCTTTGGAAGCTGTCAATCCCGGAGC
AACTTTCCCAACTACCCAACCCCACCATGGCCAGGACATGTGCAATGCCAGCCCCTTCTTGTCTTGGCACATGCACAGACCCAGTCCCCT
CACGGTAGGGCACCCCTGACCTACGGGCTTCCAAGAGAGCAGCTGCAGTGGTTGGGAGGAGCTTGACCAGTGTGCCCCAAGGAGTGGAGT
AGAGCCCAATCTAAGTATTCCTTGCTGCTTGGAACCCTCCCTGTTTGGAACCCTCCCCAAAGAGGCAGTCAGGCTGATGCTCAGTGCTTT
GTGCTCCCTGCTCCTTCCCGCGTAGCCAGGTGGGCCCAAGGGTGCCTGGCAGGGAGCACTACCCCTGGACCCCTCCTGCTCGCTCTGGGG
ACCCTGCCAGGGAAGGCCACTGGGTGTTCACCTGCAAAGTTTCTGGTTGTCACTGCACAGTGGTCGCGTCATCCATGGGTATTAAAAGGA
CACTGTCAAGTACTTTTTTAAACTAGTTTTTAGGGTTTTTTAAAACTCTCTGTTGTTTGTAATATTCTCTTAAAAGCTTGAAAATAAAAC

>78_78_1_AAMP-MARCH4_AAMP_chr2_219134105_ENST00000420660_MARCH4_chr2_217124402_ENST00000273067_length(amino acids)=194AA_BP=71
MLLSVPLTASRAPSSNPLPKADDLAQEMEDVDFEEEEEEEGNEEGWVLEPQEGVVGSMEGPDDSEVTFALHSGLIIHEGPSVYRIFKRWQ
AVNQQWKVLNYDKTKDLEDQKAGGRTNPRTSSSTQANIPSSEEETAGTPAPEQGPAQAAGHPSGPLSHHHCAYTILHILSHLRPHEQRSP

--------------------------------------------------------------

Top

Fusion Gene PPI Analysis for AAMP-MARCH4


check button Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in

ChiPPI page.


check button Protein-protein interactors with each fusion partner protein in wild-type (BIOGRID-3.4.160)
HgeneHgene's interactorsTgeneTgene's interactors


check button - Retained PPIs in in-frame fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenStill interaction with


check button - Lost PPIs in in-frame fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenInteraction lost with


check button - Retained PPIs, but lost function due to frame-shift fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenInteraction lost with


Top

Related Drugs for AAMP-MARCH4


check button Drugs targeting genes involved in this fusion gene.
(DrugBank Version 5.1.8 2021-05-08)
PartnerGeneUniProtAccDrugBank IDDrug nameDrug activityDrug typeDrug status

Top

Related Diseases for AAMP-MARCH4


check button Diseases associated with fusion partners.
(DisGeNet 4.0)
PartnerGeneDisease IDDisease name# pubmedsSource