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Fusion Gene Summary

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Fusion Gene ORF analysis

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Fusion Genomic Features

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Fusion Protein Features

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Fusion Gene Sequence

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Fusion Gene PPI analysis

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Related Drugs

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Fusion gene:AK4-WLS (FusionGDB2 ID:HG205TG79971)

Fusion Gene Summary for AK4-WLS

check button Fusion gene summary
Fusion gene informationFusion gene name: AK4-WLS
Fusion gene ID: hg205tg79971
HgeneTgene
Gene symbol

AK4

WLS

Gene ID

205

79971

Gene nameadenylate kinase 4Wnt ligand secretion mediator
SynonymsAK 4|AK3|AK3L1|AK3L2C1orf139|EVI|GPR177|MRP|mig-14
Cytomap('AK4')('WLS')

1p31.3

1p31.3

Type of geneprotein-codingprotein-coding
Descriptionadenylate kinase 4, mitochondrialATP-AMP transphosphorylaseGTP:AMP phosphotransferase AK4, mitochondrialadenylate kinase 3-like 1adenylate kinase isoenzyme 4, mitochondrialepididymis secretory sperm binding proteinmitochondrial adenylate kinase-3nuprotein wntless homologG protein-coupled receptor 177integral membrane protein GPR177protein evenness interrupted homologputative NF-kappa-B-activating protein 373putative NFkB activating protein 373wntless Wnt ligand secretion mediator
Modification date2020031520200313
UniProtAcc..
Ensembl transtripts involved in fusion geneENST00000470888, ENST00000327299, 
ENST00000395334, ENST00000545314, 
ENST00000546702, 
Fusion gene scores* DoF score2 X 2 X 2=813 X 8 X 7=728
# samples 313
** MAII scorelog2(3/8*10)=1.90689059560852log2(13/728*10)=-2.48542682717024
possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs).
DoF>8 and MAII<0
Context

PubMed: AK4 [Title/Abstract] AND WLS [Title/Abstract] AND fusion [Title/Abstract]

Most frequent breakpointAK4(65614235)-WLS(68624930), # samples:1
Anticipated loss of major functional domain due to fusion event.AK4-WLS seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF.
AK4-WLS seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF.
AK4-WLS seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF.
AK4-WLS seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF.
* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

check button Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez
PartnerGeneGO IDGO termPubMed ID
HgeneAK4

GO:0006165

nucleoside diphosphate phosphorylation

23416111

HgeneAK4

GO:0009142

nucleoside triphosphate biosynthetic process

23416111

HgeneAK4

GO:0046033

AMP metabolic process

19766732

HgeneAK4

GO:0046034

ATP metabolic process

19766732

HgeneAK4

GO:0046039

GTP metabolic process

19766732


check buttonFusion gene breakpoints across AK4 (5'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.
all structure
check buttonFusion gene breakpoints across WLS (3'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.
all structure

check button Fusion gene information
* All genome coordinats were lifted-over on hg19.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.
SourceDiseaseSampleHgeneHchrHbpHstrandTgeneTchrTbpTstrand
ChimerDB4SARCTCGA-DX-AATS-01AAK4chr1

65614235

+WLSchr1

68624930

-


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Fusion Gene ORF analysis for AK4-WLS

check button Open reading frame (ORF) analsis of fusion genes based on Ensembl gene isoform structure.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.
ORFHenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrand
3UTR-3CDSENST00000470888ENST00000262348AK4chr1

65614235

+WLSchr1

68624930

-
3UTR-3CDSENST00000470888ENST00000354777AK4chr1

65614235

+WLSchr1

68624930

-
3UTR-3CDSENST00000470888ENST00000370976AK4chr1

65614235

+WLSchr1

68624930

-
3UTR-3CDSENST00000470888ENST00000540432AK4chr1

65614235

+WLSchr1

68624930

-
3UTR-intronENST00000470888ENST00000370971AK4chr1

65614235

+WLSchr1

68624930

-
3UTR-intronENST00000470888ENST00000491811AK4chr1

65614235

+WLSchr1

68624930

-
5CDS-intronENST00000327299ENST00000370971AK4chr1

65614235

+WLSchr1

68624930

-
5CDS-intronENST00000327299ENST00000491811AK4chr1

65614235

+WLSchr1

68624930

-
5CDS-intronENST00000395334ENST00000370971AK4chr1

65614235

+WLSchr1

68624930

-
5CDS-intronENST00000395334ENST00000491811AK4chr1

65614235

+WLSchr1

68624930

-
5CDS-intronENST00000545314ENST00000370971AK4chr1

65614235

+WLSchr1

68624930

-
5CDS-intronENST00000545314ENST00000491811AK4chr1

65614235

+WLSchr1

68624930

-
In-frameENST00000327299ENST00000262348AK4chr1

65614235

+WLSchr1

68624930

-
In-frameENST00000327299ENST00000354777AK4chr1

65614235

+WLSchr1

68624930

-
In-frameENST00000327299ENST00000370976AK4chr1

65614235

+WLSchr1

68624930

-
In-frameENST00000327299ENST00000540432AK4chr1

65614235

+WLSchr1

68624930

-
In-frameENST00000395334ENST00000262348AK4chr1

65614235

+WLSchr1

68624930

-
In-frameENST00000395334ENST00000354777AK4chr1

65614235

+WLSchr1

68624930

-
In-frameENST00000395334ENST00000370976AK4chr1

65614235

+WLSchr1

68624930

-
In-frameENST00000395334ENST00000540432AK4chr1

65614235

+WLSchr1

68624930

-
In-frameENST00000545314ENST00000262348AK4chr1

65614235

+WLSchr1

68624930

-
In-frameENST00000545314ENST00000354777AK4chr1

65614235

+WLSchr1

68624930

-
In-frameENST00000545314ENST00000370976AK4chr1

65614235

+WLSchr1

68624930

-
In-frameENST00000545314ENST00000540432AK4chr1

65614235

+WLSchr1

68624930

-
intron-3CDSENST00000546702ENST00000262348AK4chr1

65614235

+WLSchr1

68624930

-
intron-3CDSENST00000546702ENST00000354777AK4chr1

65614235

+WLSchr1

68624930

-
intron-3CDSENST00000546702ENST00000370976AK4chr1

65614235

+WLSchr1

68624930

-
intron-3CDSENST00000546702ENST00000540432AK4chr1

65614235

+WLSchr1

68624930

-
intron-intronENST00000546702ENST00000370971AK4chr1

65614235

+WLSchr1

68624930

-
intron-intronENST00000546702ENST00000491811AK4chr1

65614235

+WLSchr1

68624930

-

check buttonORFfinder result based on the fusion transcript sequence of in-frame fusion genes.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandSeq length
(transcript)		BP loci
(transcript)		Predicted start
(transcript)		Predicted stop
(transcript)		Seq length
(amino acids)
ENST00000545314AK4chr165614235+ENST00000540432WLSchr168624930-18243922201650476
ENST00000545314AK4chr165614235+ENST00000354777WLSchr168624930-18103922201650476
ENST00000545314AK4chr165614235+ENST00000262348WLSchr168624930-25073922201638472
ENST00000545314AK4chr165614235+ENST00000370976WLSchr168624930-23733922201638472
ENST00000395334AK4chr165614235+ENST00000540432WLSchr168624930-19355033311761476
ENST00000395334AK4chr165614235+ENST00000354777WLSchr168624930-19215033311761476
ENST00000395334AK4chr165614235+ENST00000262348WLSchr168624930-26185033311749472
ENST00000395334AK4chr165614235+ENST00000370976WLSchr168624930-24845033311749472
ENST00000327299AK4chr165614235+ENST00000540432WLSchr168624930-178235071608533
ENST00000327299AK4chr165614235+ENST00000354777WLSchr168624930-176835071608533
ENST00000327299AK4chr165614235+ENST00000262348WLSchr168624930-246535071596529
ENST00000327299AK4chr165614235+ENST00000370976WLSchr168624930-233135071596529

check buttonDeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandNo-coding scoreCoding score
ENST00000545314ENST00000540432AK4chr165614235+WLSchr168624930-0.0026887180.99731135
ENST00000545314ENST00000354777AK4chr165614235+WLSchr168624930-0.0027620320.9972379
ENST00000545314ENST00000262348AK4chr165614235+WLSchr168624930-0.0012249260.99877506
ENST00000545314ENST00000370976AK4chr165614235+WLSchr168624930-0.0014422940.99855775
ENST00000395334ENST00000540432AK4chr165614235+WLSchr168624930-0.0038234590.9961765
ENST00000395334ENST00000354777AK4chr165614235+WLSchr168624930-0.0039965490.99600345
ENST00000395334ENST00000262348AK4chr165614235+WLSchr168624930-0.0016881380.9983119
ENST00000395334ENST00000370976AK4chr165614235+WLSchr168624930-0.002070260.9979297
ENST00000327299ENST00000540432AK4chr165614235+WLSchr168624930-0.0025268560.9974732
ENST00000327299ENST00000354777AK4chr165614235+WLSchr168624930-0.0026342080.9973658
ENST00000327299ENST00000262348AK4chr165614235+WLSchr168624930-0.0010311430.99896884
ENST00000327299ENST00000370976AK4chr165614235+WLSchr168624930-0.001171920.9988281

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Fusion Genomic Features for AK4-WLS


check buttonFusionAI prediction of the potential fusion gene breakpoint based on the pre-mature RNA sequence context (+/- 5kb of individual partner genes, total 20kb length sequence). FusionAI is a fusion gene breakpoint classifier based on convolutional neural network by comparing the fusion positive and negative sequence context of ~ 20K fusion gene data. From here, we can have the relative potentency of the 20K genomic sequence how individual sequnce will be likely used as the gene fusion breakpoints.
HgeneHchrHbpHstrandTgeneTchrTbpTstrand1-pp (fusion gene breakpoint)

check buttonDistribution of 44 human genomic features loci across 20kb length fusion breakpoint regions. We integrated a total of 44 different types of human genomic feature loci information across five big categories including virus integration sites, repeats, structural variants, chromatin states, and gene expression regulation. More details are in help page.
genomic feature

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Fusion Protein Features for AK4-WLS


check button Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chr1:65614235/chr1:68624930)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
4. Go down and press 'Search' tab twice.
4. Go down to have the hyperlink of the search result.
5. Click the hyperlink.
6. See the FGviewer result for your fusion gene.
FGviewer

check buttonMain function of each fusion partner protein. (from UniProt)
HgeneTgene
..
FUNCTION: Transcriptional activator which is required for calcium-dependent dendritic growth and branching in cortical neurons. Recruits CREB-binding protein (CREBBP) to nuclear bodies. Component of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating a calcium-dependent release of a repressor complex and a recruitment of an activator complex. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex. At the same time, there is increased recruitment of CREBBP to the promoter by a CREST-dependent mechanism, which leads to transcriptional activation. The CREST-BRG1 complex also binds to the NR2B promoter, and activity-dependent induction of NR2B expression involves a release of HDAC1 and recruitment of CREBBP (By similarity). {ECO:0000250}.FUNCTION: Transcriptional activator which is required for calcium-dependent dendritic growth and branching in cortical neurons. Recruits CREB-binding protein (CREBBP) to nuclear bodies. Component of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating a calcium-dependent release of a repressor complex and a recruitment of an activator complex. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex. At the same time, there is increased recruitment of CREBBP to the promoter by a CREST-dependent mechanism, which leads to transcriptional activation. The CREST-BRG1 complex also binds to the NR2B promoter, and activity-dependent induction of NR2B expression involves a release of HDAC1 and recruitment of CREBBP (By similarity). {ECO:0000250}.

check buttonRetention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at

download page


* Minus value of BPloci means that the break pointn is located before the CDS.
- In-frame and retained protein feature among the 13 regional features.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note
HgeneAK4chr1:65614235chr1:68624930ENST00000327299+1515_2048224.0Nucleotide bindingNTP
HgeneAK4chr1:65614235chr1:68624930ENST00000395334+2615_2048224.0Nucleotide bindingNTP
HgeneAK4chr1:65614235chr1:68624930ENST00000545314+2615_2048224.0Nucleotide bindingNTP
TgeneWLSchr1:65614235chr1:68624930ENST00000262348112254_268126542.0Topological domainCytoplasmic
TgeneWLSchr1:65614235chr1:68624930ENST00000262348112290_303126542.0Topological domainLumenal
TgeneWLSchr1:65614235chr1:68624930ENST00000262348112325_331126542.0Topological domainCytoplasmic
TgeneWLSchr1:65614235chr1:68624930ENST00000262348112353_380126542.0Topological domainLumenal
TgeneWLSchr1:65614235chr1:68624930ENST00000262348112402_431126542.0Topological domainCytoplasmic
TgeneWLSchr1:65614235chr1:68624930ENST00000262348112453_471126542.0Topological domainLumenal
TgeneWLSchr1:65614235chr1:68624930ENST00000262348112493_541126542.0Topological domainCytoplasmic
TgeneWLSchr1:65614235chr1:68624930ENST00000354777112254_268124544.0Topological domainCytoplasmic
TgeneWLSchr1:65614235chr1:68624930ENST00000354777112290_303124544.0Topological domainLumenal
TgeneWLSchr1:65614235chr1:68624930ENST00000354777112325_331124544.0Topological domainCytoplasmic
TgeneWLSchr1:65614235chr1:68624930ENST00000354777112353_380124544.0Topological domainLumenal
TgeneWLSchr1:65614235chr1:68624930ENST00000354777112402_431124544.0Topological domainCytoplasmic
TgeneWLSchr1:65614235chr1:68624930ENST00000354777112453_471124544.0Topological domainLumenal
TgeneWLSchr1:65614235chr1:68624930ENST00000354777112493_541124544.0Topological domainCytoplasmic
TgeneWLSchr1:65614235chr1:68624930ENST00000370976011254_26835451.0Topological domainCytoplasmic
TgeneWLSchr1:65614235chr1:68624930ENST00000370976011290_30335451.0Topological domainLumenal
TgeneWLSchr1:65614235chr1:68624930ENST00000370976011325_33135451.0Topological domainCytoplasmic
TgeneWLSchr1:65614235chr1:68624930ENST00000370976011353_38035451.0Topological domainLumenal
TgeneWLSchr1:65614235chr1:68624930ENST0000037097601137_23235451.0Topological domainLumenal
TgeneWLSchr1:65614235chr1:68624930ENST00000370976011402_43135451.0Topological domainCytoplasmic
TgeneWLSchr1:65614235chr1:68624930ENST00000370976011453_47135451.0Topological domainLumenal
TgeneWLSchr1:65614235chr1:68624930ENST00000370976011493_54135451.0Topological domainCytoplasmic
TgeneWLSchr1:65614235chr1:68624930ENST00000262348112233_253126542.0TransmembraneHelical%3B Name%3D2
TgeneWLSchr1:65614235chr1:68624930ENST00000262348112269_289126542.0TransmembraneHelical%3B Name%3D3
TgeneWLSchr1:65614235chr1:68624930ENST00000262348112304_324126542.0TransmembraneHelical%3B Name%3D4
TgeneWLSchr1:65614235chr1:68624930ENST00000262348112332_352126542.0TransmembraneHelical%3B Name%3D5
TgeneWLSchr1:65614235chr1:68624930ENST00000262348112381_401126542.0TransmembraneHelical%3B Name%3D6
TgeneWLSchr1:65614235chr1:68624930ENST00000262348112432_452126542.0TransmembraneHelical%3B Name%3D7
TgeneWLSchr1:65614235chr1:68624930ENST00000262348112472_492126542.0TransmembraneHelical%3B Name%3D8
TgeneWLSchr1:65614235chr1:68624930ENST00000354777112233_253124544.0TransmembraneHelical%3B Name%3D2
TgeneWLSchr1:65614235chr1:68624930ENST00000354777112269_289124544.0TransmembraneHelical%3B Name%3D3
TgeneWLSchr1:65614235chr1:68624930ENST00000354777112304_324124544.0TransmembraneHelical%3B Name%3D4
TgeneWLSchr1:65614235chr1:68624930ENST00000354777112332_352124544.0TransmembraneHelical%3B Name%3D5
TgeneWLSchr1:65614235chr1:68624930ENST00000354777112381_401124544.0TransmembraneHelical%3B Name%3D6
TgeneWLSchr1:65614235chr1:68624930ENST00000354777112432_452124544.0TransmembraneHelical%3B Name%3D7
TgeneWLSchr1:65614235chr1:68624930ENST00000354777112472_492124544.0TransmembraneHelical%3B Name%3D8
TgeneWLSchr1:65614235chr1:68624930ENST00000370976011233_25335451.0TransmembraneHelical%3B Name%3D2
TgeneWLSchr1:65614235chr1:68624930ENST00000370976011269_28935451.0TransmembraneHelical%3B Name%3D3
TgeneWLSchr1:65614235chr1:68624930ENST00000370976011304_32435451.0TransmembraneHelical%3B Name%3D4
TgeneWLSchr1:65614235chr1:68624930ENST00000370976011332_35235451.0TransmembraneHelical%3B Name%3D5
TgeneWLSchr1:65614235chr1:68624930ENST00000370976011381_40135451.0TransmembraneHelical%3B Name%3D6
TgeneWLSchr1:65614235chr1:68624930ENST00000370976011432_45235451.0TransmembraneHelical%3B Name%3D7
TgeneWLSchr1:65614235chr1:68624930ENST00000370976011472_49235451.0TransmembraneHelical%3B Name%3D8

- In-frame and not-retained protein feature among the 13 regional features.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note
HgeneAK4chr1:65614235chr1:68624930ENST00000327299+15135_13648224.0Nucleotide bindingNTP
HgeneAK4chr1:65614235chr1:68624930ENST00000327299+1562_6448224.0Nucleotide bindingAMP
HgeneAK4chr1:65614235chr1:68624930ENST00000327299+1589_9248224.0Nucleotide bindingAMP
HgeneAK4chr1:65614235chr1:68624930ENST00000395334+26135_13648224.0Nucleotide bindingNTP
HgeneAK4chr1:65614235chr1:68624930ENST00000395334+2662_6448224.0Nucleotide bindingAMP
HgeneAK4chr1:65614235chr1:68624930ENST00000395334+2689_9248224.0Nucleotide bindingAMP
HgeneAK4chr1:65614235chr1:68624930ENST00000545314+26135_13648224.0Nucleotide bindingNTP
HgeneAK4chr1:65614235chr1:68624930ENST00000545314+2662_6448224.0Nucleotide bindingAMP
HgeneAK4chr1:65614235chr1:68624930ENST00000545314+2689_9248224.0Nucleotide bindingAMP
HgeneAK4chr1:65614235chr1:68624930ENST00000327299+15125_16248224.0RegionLID
HgeneAK4chr1:65614235chr1:68624930ENST00000327299+1535_6448224.0RegionNMP
HgeneAK4chr1:65614235chr1:68624930ENST00000395334+26125_16248224.0RegionLID
HgeneAK4chr1:65614235chr1:68624930ENST00000395334+2635_6448224.0RegionNMP
HgeneAK4chr1:65614235chr1:68624930ENST00000545314+26125_16248224.0RegionLID
HgeneAK4chr1:65614235chr1:68624930ENST00000545314+2635_6448224.0RegionNMP
TgeneWLSchr1:65614235chr1:68624930ENST000002623481121_15126542.0Topological domainCytoplasmic
TgeneWLSchr1:65614235chr1:68624930ENST0000026234811237_232126542.0Topological domainLumenal
TgeneWLSchr1:65614235chr1:68624930ENST000003547771121_15124544.0Topological domainCytoplasmic
TgeneWLSchr1:65614235chr1:68624930ENST0000035477711237_232124544.0Topological domainLumenal
TgeneWLSchr1:65614235chr1:68624930ENST000003709760111_1535451.0Topological domainCytoplasmic
TgeneWLSchr1:65614235chr1:68624930ENST0000026234811216_36126542.0TransmembraneHelical%3B Name%3D1
TgeneWLSchr1:65614235chr1:68624930ENST0000035477711216_36124544.0TransmembraneHelical%3B Name%3D1
TgeneWLSchr1:65614235chr1:68624930ENST0000037097601116_3635451.0TransmembraneHelical%3B Name%3D1


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Fusion Gene Sequence for AK4-WLS


check button For in-frame fusion transcripts, we provide the fusion transcript sequences and fusion amino acid sequences. To have fusion amino acid sequence, we ran ORFfinder and chose the longest ORF among the all predicted ones.
>3245_3245_1_AK4-WLS_AK4_chr1_65614235_ENST00000327299_WLS_chr1_68624930_ENST00000262348_length(transcript)=2465nt_BP=350nt
AGTCCGCCTGCTACTCGGTCCCGGCGCTGGGCTGAGGGGAGGGGTTGTCTTAAAAGTCTCTCCTTCCCCCTGTAGGGGCGGCCGGCGAGT
CCCAGTGAGAGCGGAGGGTGCCAGAGGTAGGGGGCCGAGAAACAAAGTTCCCGGGGCTTCCTCCGGGGCCGCGGTCGGGGCTGCGCGTTT
GACCGCCCCCCTCCTCGCGAAGGCAATGGCTTCCAAACTCCTGCGCGCGGTCATCCTCGGGCCGCCCGGCTCGGGCAAGGGCACCGTGTG
CCAGAGGATCGCCCAGAACTTTGGTCTCCAGCATCTCTCCAGCGGCCACTTCTTGCGGGAGAACATCAAGGCCAGCACCGGAGAAAATGC
AGAAGTCTCCATGGACGTTTCCCTGGCTTACCGTGATGACGCGTTTGCTGAGTGGACTGAAATGGCCCATGAAAGAGTACCACGGAAACT
CAAATGCACCTTCACATCTCCCAAGACTCCAGAGCATGAGGGCCGTTACTATGAATGTGATGTCCTTCCTTTCATGGAAATTGGGTCTGT
GGCCCATAAGTTTTACCTTTTAAACATCCGGCTGCCTGTGAATGAGAAGAAGAAAATCAATGTGGGAATTGGGGAGATAAAGGATATCCG
GTTGGTGGGGATCCACCAAAATGGAGGCTTCACCAAGGTGTGGTTTGCCATGAAGACCTTCCTTACGCCCAGCATCTTCATCATTATGGT
GTGGTATTGGAGGAGGATCACCATGATGTCCCGACCCCCAGTGCTTCTGGAAAAAGTCATCTTTGCCCTTGGGATTTCCATGACCTTTAT
CAATATCCCAGTGGAATGGTTTTCCATCGGGTTTGACTGGACCTGGATGCTGCTGTTTGGTGACATCCGACAGGGCATCTTCTATGCGAT
GCTTCTGTCCTTCTGGATCATCTTCTGTGGCGAGCACATGATGGATCAGCACGAGCGGAACCACATCGCAGGGTATTGGAAGCAAGTCGG
ACCCATTGCCGTTGGCTCCTTCTGCCTCTTCATATTTGACATGTGTGAGAGAGGGGTACAACTCACGAATCCCTTCTACAGTATCTGGAC
TACAGACATTGGAACAGAGCTGGCCATGGCCTTCATCATCGTGGCTGGAATCTGCCTCTGCCTCTACTTCCTGTTTCTATGCTTCATGGT
ATTTCAGGTGTTTCGGAACATCAGTGGGAAGCAGTCCAGCCTGCCAGCTATGAGCAAAGTCCGGCGGCTACACTATGAGGGGCTAATTTT
TAGGTTCAAGTTCCTCATGCTTATCACCTTGGCCTGCGCTGCCATGACTGTCATCTTCTTCATCGTTAGTCAGGTAACGGAAGGCCATTG
GAAATGGGGCGGCGTCACAGTCCAAGTGAACAGTGCCTTTTTCACAGGCATCTATGGGATGTGGAATCTGTATGTCTTTGCTCTGATGTT
CTTGTATGCACCATCCCATAAAAACTATGGAGAAGACCAGTCCAATGGCGATCTGGGTGTCCATAGTGGGGAAGAACTCCAGCTCACCAC
CACTATCACCCATGTGGACGGACCCACTGAGATCTACAAGTTGACCCGCAAGGAGGCCCAGGAGTAGGAGGCTGCAGCGCCCGGCTGGGA
CGGTCTCTCCATACCCCAGCCCCTCTAACTAGAGTGGGGAGCATGCCAGAGAGAGCTCAATGTACAAATGAATGCCTCATGGCTCTTAGC
TGTGGTTTCTTGGACCAGCGGCATGGACATTTGTCAGTTTGCCTTCTGACGGTAGCTTTTGGAGGAAGATTCCTGCAGCCACTAATGCAT
TGTGTATGATAACAAAAACTCTGGTATGACACATTTTCTGTGATCATTGTTAATTAGTGACATAGTAACATCTGTAGCAGCTGGTTAGTA
AACCTCATGTGGGGGTGGGGTGGGGGTGTATTCCTTGGGGGATGGTTTGGGCCGAATGGGGAGTGGAATATTTGACATTTTTCCTGTTTT
AAATTCTAGGATAGATTTTAACATCCTTTGCGGTCCCAGTCCAAGGTAGGCTGGTGTCATAGTCTTCTCACTCCTAATCCATGACCACTG
TTTTTTTCCTATTTATATCACCAGGTAGCCCACTGAGTTAATATTTAAGTTGTCAATAGATAAGTGTCCCTGTTTTGTGGCATAATATAA
CTGAATTTCATGAGAAGATTTATTCCACCAGGGGTATTTCAGCTTTGAAACCAAATCTGTGTATCTAATACTAACCAATCTGTTGGATGT
GGGTTTTAAAAAATGTTTGCTAAACTACCCAAGTAAGATTTACTGTATTAAATGGCCTTCGGGTCTGAAAAGCTTTTTTAACTTCTTGCT
TAAAATGCGTTTTATTTTGATAAGATACTTCAAATAGCCTCCAAAAGTGTAGATCCAATCATTTAAATAAACCTGTATGTATATGCATAT

>3245_3245_1_AK4-WLS_AK4_chr1_65614235_ENST00000327299_WLS_chr1_68624930_ENST00000262348_length(amino acids)=529AA_BP=108
MLLGPGAGLRGGVVLKVSPSPCRGGRRVPVRAEGARGRGPRNKVPGASSGAAVGAARLTAPLLAKAMASKLLRAVILGPPGSGKGTVCQR
IAQNFGLQHLSSGHFLRENIKASTGENAEVSMDVSLAYRDDAFAEWTEMAHERVPRKLKCTFTSPKTPEHEGRYYECDVLPFMEIGSVAH
KFYLLNIRLPVNEKKKINVGIGEIKDIRLVGIHQNGGFTKVWFAMKTFLTPSIFIIMVWYWRRITMMSRPPVLLEKVIFALGISMTFINI
PVEWFSIGFDWTWMLLFGDIRQGIFYAMLLSFWIIFCGEHMMDQHERNHIAGYWKQVGPIAVGSFCLFIFDMCERGVQLTNPFYSIWTTD
IGTELAMAFIIVAGICLCLYFLFLCFMVFQVFRNISGKQSSLPAMSKVRRLHYEGLIFRFKFLMLITLACAAMTVIFFIVSQVTEGHWKW

--------------------------------------------------------------
>3245_3245_2_AK4-WLS_AK4_chr1_65614235_ENST00000327299_WLS_chr1_68624930_ENST00000354777_length(transcript)=1768nt_BP=350nt
AGTCCGCCTGCTACTCGGTCCCGGCGCTGGGCTGAGGGGAGGGGTTGTCTTAAAAGTCTCTCCTTCCCCCTGTAGGGGCGGCCGGCGAGT
CCCAGTGAGAGCGGAGGGTGCCAGAGGTAGGGGGCCGAGAAACAAAGTTCCCGGGGCTTCCTCCGGGGCCGCGGTCGGGGCTGCGCGTTT
GACCGCCCCCCTCCTCGCGAAGGCAATGGCTTCCAAACTCCTGCGCGCGGTCATCCTCGGGCCGCCCGGCTCGGGCAAGGGCACCGTGTG
CCAGAGGATCGCCCAGAACTTTGGTCTCCAGCATCTCTCCAGCGGCCACTTCTTGCGGGAGAACATCAAGGCCAGCACCGGAGAAAATGC
AGAAGTCTCCATGGACGTTTCCCTGGCTTACCGTGATGACGCGTTTGCTGAGTGGACTGAAATGGCCCATGAAAGAGTACCACGGAAACT
CAAATGCACCTTCACATCTCCCAAGACTCCAGAGCATGAGGGCCGTTACTATGAATGTGATGTCCTTCCTTTCATGGAAATTGGGTCTGT
GGCCCATAAGTTTTACCTTTTAAACATCCGGCTGCCTGTGAATGAGAAGAAGAAAATCAATGTGGGAATTGGGGAGATAAAGGATATCCG
GTTGGTGGGGATCCACCAAAATGGAGGCTTCACCAAGGTGTGGTTTGCCATGAAGACCTTCCTTACGCCCAGCATCTTCATCATTATGGT
GTGGTATTGGAGGAGGATCACCATGATGTCCCGACCCCCAGTGCTTCTGGAAAAAGTCATCTTTGCCCTTGGGATTTCCATGACCTTTAT
CAATATCCCAGTGGAATGGTTTTCCATCGGGTTTGACTGGACCTGGATGCTGCTGTTTGGTGACATCCGACAGGGCATCTTCTATGCGAT
GCTTCTGTCCTTCTGGATCATCTTCTGTGGCGAGCACATGATGGATCAGCACGAGCGGAACCACATCGCAGGGTATTGGAAGCAAGTCGG
ACCCATTGCCGTTGGCTCCTTCTGCCTCTTCATATTTGACATGTGTGAGAGAGGGGTACAACTCACGAATCCCTTCTACAGTATCTGGAC
TACAGACATTGGAACAGAGCTGGCCATGGCCTTCATCATCGTGGCTGGAATCTGCCTCTGCCTCTACTTCCTGTTTCTATGCTTCATGGT
ATTTCAGGTGTTTCGGAACATCAGTGGGAAGCAGTCCAGCCTGCCAGCTATGAGCAAAGTCCGGCGGCTACACTATGAGGGGCTAATTTT
TAGGTTCAAGTTCCTCATGCTTATCACCTTGGCCTGCGCTGCCATGACTGTCATCTTCTTCATCGTTAGTCAGGTAACGGAAGGCCATTG
GAAATGGGGCGGCGTCACAGTCCAAGTGAACAGTGCCTTTTTCACAGGCATCTATGGGATGTGGAATCTGTATGTCTTTGCTCTGATGTT
CTTGTATGCACCATCCCATAAAAACTATGGAGAAGACCAGTCCAATGGAATGCAACTCCCATGTAAATCGAGGGAAGATTGTGCTTTGTT
TGTTTCGGAACTTTATCAAGAATTGTTCAGCGCTTCGAAATATTCCTTCATCAATGACAACGCAGCTTCTGGTATTTGAGTCAACAAGGC
AACACATGTTTATCAGCTTTGCATTTGCAGTTGTCACAGTCACATTGATTGTACTTGTATACGCACACAAATACACTCATTTAGCCTTTA

>3245_3245_2_AK4-WLS_AK4_chr1_65614235_ENST00000327299_WLS_chr1_68624930_ENST00000354777_length(amino acids)=533AA_BP=108
MLLGPGAGLRGGVVLKVSPSPCRGGRRVPVRAEGARGRGPRNKVPGASSGAAVGAARLTAPLLAKAMASKLLRAVILGPPGSGKGTVCQR
IAQNFGLQHLSSGHFLRENIKASTGENAEVSMDVSLAYRDDAFAEWTEMAHERVPRKLKCTFTSPKTPEHEGRYYECDVLPFMEIGSVAH
KFYLLNIRLPVNEKKKINVGIGEIKDIRLVGIHQNGGFTKVWFAMKTFLTPSIFIIMVWYWRRITMMSRPPVLLEKVIFALGISMTFINI
PVEWFSIGFDWTWMLLFGDIRQGIFYAMLLSFWIIFCGEHMMDQHERNHIAGYWKQVGPIAVGSFCLFIFDMCERGVQLTNPFYSIWTTD
IGTELAMAFIIVAGICLCLYFLFLCFMVFQVFRNISGKQSSLPAMSKVRRLHYEGLIFRFKFLMLITLACAAMTVIFFIVSQVTEGHWKW

--------------------------------------------------------------
>3245_3245_3_AK4-WLS_AK4_chr1_65614235_ENST00000327299_WLS_chr1_68624930_ENST00000370976_length(transcript)=2331nt_BP=350nt
AGTCCGCCTGCTACTCGGTCCCGGCGCTGGGCTGAGGGGAGGGGTTGTCTTAAAAGTCTCTCCTTCCCCCTGTAGGGGCGGCCGGCGAGT
CCCAGTGAGAGCGGAGGGTGCCAGAGGTAGGGGGCCGAGAAACAAAGTTCCCGGGGCTTCCTCCGGGGCCGCGGTCGGGGCTGCGCGTTT
GACCGCCCCCCTCCTCGCGAAGGCAATGGCTTCCAAACTCCTGCGCGCGGTCATCCTCGGGCCGCCCGGCTCGGGCAAGGGCACCGTGTG
CCAGAGGATCGCCCAGAACTTTGGTCTCCAGCATCTCTCCAGCGGCCACTTCTTGCGGGAGAACATCAAGGCCAGCACCGGAGAAAATGC
AGAAGTCTCCATGGACGTTTCCCTGGCTTACCGTGATGACGCGTTTGCTGAGTGGACTGAAATGGCCCATGAAAGAGTACCACGGAAACT
CAAATGCACCTTCACATCTCCCAAGACTCCAGAGCATGAGGGCCGTTACTATGAATGTGATGTCCTTCCTTTCATGGAAATTGGGTCTGT
GGCCCATAAGTTTTACCTTTTAAACATCCGGCTGCCTGTGAATGAGAAGAAGAAAATCAATGTGGGAATTGGGGAGATAAAGGATATCCG
GTTGGTGGGGATCCACCAAAATGGAGGCTTCACCAAGGTGTGGTTTGCCATGAAGACCTTCCTTACGCCCAGCATCTTCATCATTATGGT
GTGGTATTGGAGGAGGATCACCATGATGTCCCGACCCCCAGTGCTTCTGGAAAAAGTCATCTTTGCCCTTGGGATTTCCATGACCTTTAT
CAATATCCCAGTGGAATGGTTTTCCATCGGGTTTGACTGGACCTGGATGCTGCTGTTTGGTGACATCCGACAGGGCATCTTCTATGCGAT
GCTTCTGTCCTTCTGGATCATCTTCTGTGGCGAGCACATGATGGATCAGCACGAGCGGAACCACATCGCAGGGTATTGGAAGCAAGTCGG
ACCCATTGCCGTTGGCTCCTTCTGCCTCTTCATATTTGACATGTGTGAGAGAGGGGTACAACTCACGAATCCCTTCTACAGTATCTGGAC
TACAGACATTGGAACAGAGCTGGCCATGGCCTTCATCATCGTGGCTGGAATCTGCCTCTGCCTCTACTTCCTGTTTCTATGCTTCATGGT
ATTTCAGGTGTTTCGGAACATCAGTGGGAAGCAGTCCAGCCTGCCAGCTATGAGCAAAGTCCGGCGGCTACACTATGAGGGGCTAATTTT
TAGGTTCAAGTTCCTCATGCTTATCACCTTGGCCTGCGCTGCCATGACTGTCATCTTCTTCATCGTTAGTCAGGTAACGGAAGGCCATTG
GAAATGGGGCGGCGTCACAGTCCAAGTGAACAGTGCCTTTTTCACAGGCATCTATGGGATGTGGAATCTGTATGTCTTTGCTCTGATGTT
CTTGTATGCACCATCCCATAAAAACTATGGAGAAGACCAGTCCAATGGCGATCTGGGTGTCCATAGTGGGGAAGAACTCCAGCTCACCAC
CACTATCACCCATGTGGACGGACCCACTGAGATCTACAAGTTGACCCGCAAGGAGGCCCAGGAGTAGGAGGCTGCAGCGCCCGGCTGGGA
CGGTCTCTCCATACCCCAGCCCCTCTAACTAGAGTGGGGAGCATGCCAGAGAGAGCTCAATGTACAAATGAATGCCTCATGGCTCTTAGC
TGTGGTTTCTTGGACCAGCGGCATGGACATTTGTCAGTTTGCCTTCTGACGGTAGCTTTTGGAGGAAGATTCCTGCAGCCACTAATGCAT
TGTGTATGATAACAAAAACTCTGGTATGACACATTTTCTGTGATCATTGTTAATTAGTGACATAGTAACATCTGTAGCAGCTGGTTAGTA
AACCTCATGTGGGGGTGGGGTGGGGGTGTATTCCTTGGGGGATGGTTTGGGCCGAATGGGGAGTGGAATATTTGACATTTTTCCTGTTTT
AAATTCTAGGATAGATTTTAACATCCTTTGCGGTCCCAGTCCAAGGTAGGCTGGTGTCATAGTCTTCTCACTCCTAATCCATGACCACTG
TTTTTTTCCTATTTATATCACCAGGTAGCCCACTGAGTTAATATTTAAGTTGTCAATAGATAAGTGTCCCTGTTTTGTGGCATAATATAA
CTGAATTTCATGAGAAGATTTATTCCACCAGGGGTATTTCAGCTTTGAAACCAAATCTGTGTATCTAATACTAACCAATCTGTTGGATGT

>3245_3245_3_AK4-WLS_AK4_chr1_65614235_ENST00000327299_WLS_chr1_68624930_ENST00000370976_length(amino acids)=529AA_BP=108
MLLGPGAGLRGGVVLKVSPSPCRGGRRVPVRAEGARGRGPRNKVPGASSGAAVGAARLTAPLLAKAMASKLLRAVILGPPGSGKGTVCQR
IAQNFGLQHLSSGHFLRENIKASTGENAEVSMDVSLAYRDDAFAEWTEMAHERVPRKLKCTFTSPKTPEHEGRYYECDVLPFMEIGSVAH
KFYLLNIRLPVNEKKKINVGIGEIKDIRLVGIHQNGGFTKVWFAMKTFLTPSIFIIMVWYWRRITMMSRPPVLLEKVIFALGISMTFINI
PVEWFSIGFDWTWMLLFGDIRQGIFYAMLLSFWIIFCGEHMMDQHERNHIAGYWKQVGPIAVGSFCLFIFDMCERGVQLTNPFYSIWTTD
IGTELAMAFIIVAGICLCLYFLFLCFMVFQVFRNISGKQSSLPAMSKVRRLHYEGLIFRFKFLMLITLACAAMTVIFFIVSQVTEGHWKW

--------------------------------------------------------------
>3245_3245_4_AK4-WLS_AK4_chr1_65614235_ENST00000327299_WLS_chr1_68624930_ENST00000540432_length(transcript)=1782nt_BP=350nt
AGTCCGCCTGCTACTCGGTCCCGGCGCTGGGCTGAGGGGAGGGGTTGTCTTAAAAGTCTCTCCTTCCCCCTGTAGGGGCGGCCGGCGAGT
CCCAGTGAGAGCGGAGGGTGCCAGAGGTAGGGGGCCGAGAAACAAAGTTCCCGGGGCTTCCTCCGGGGCCGCGGTCGGGGCTGCGCGTTT
GACCGCCCCCCTCCTCGCGAAGGCAATGGCTTCCAAACTCCTGCGCGCGGTCATCCTCGGGCCGCCCGGCTCGGGCAAGGGCACCGTGTG
CCAGAGGATCGCCCAGAACTTTGGTCTCCAGCATCTCTCCAGCGGCCACTTCTTGCGGGAGAACATCAAGGCCAGCACCGGAGAAAATGC
AGAAGTCTCCATGGACGTTTCCCTGGCTTACCGTGATGACGCGTTTGCTGAGTGGACTGAAATGGCCCATGAAAGAGTACCACGGAAACT
CAAATGCACCTTCACATCTCCCAAGACTCCAGAGCATGAGGGCCGTTACTATGAATGTGATGTCCTTCCTTTCATGGAAATTGGGTCTGT
GGCCCATAAGTTTTACCTTTTAAACATCCGGCTGCCTGTGAATGAGAAGAAGAAAATCAATGTGGGAATTGGGGAGATAAAGGATATCCG
GTTGGTGGGGATCCACCAAAATGGAGGCTTCACCAAGGTGTGGTTTGCCATGAAGACCTTCCTTACGCCCAGCATCTTCATCATTATGGT
GTGGTATTGGAGGAGGATCACCATGATGTCCCGACCCCCAGTGCTTCTGGAAAAAGTCATCTTTGCCCTTGGGATTTCCATGACCTTTAT
CAATATCCCAGTGGAATGGTTTTCCATCGGGTTTGACTGGACCTGGATGCTGCTGTTTGGTGACATCCGACAGGGCATCTTCTATGCGAT
GCTTCTGTCCTTCTGGATCATCTTCTGTGGCGAGCACATGATGGATCAGCACGAGCGGAACCACATCGCAGGGTATTGGAAGCAAGTCGG
ACCCATTGCCGTTGGCTCCTTCTGCCTCTTCATATTTGACATGTGTGAGAGAGGGGTACAACTCACGAATCCCTTCTACAGTATCTGGAC
TACAGACATTGGAACAGAGCTGGCCATGGCCTTCATCATCGTGGCTGGAATCTGCCTCTGCCTCTACTTCCTGTTTCTATGCTTCATGGT
ATTTCAGGTGTTTCGGAACATCAGTGGGAAGCAGTCCAGCCTGCCAGCTATGAGCAAAGTCCGGCGGCTACACTATGAGGGGCTAATTTT
TAGGTTCAAGTTCCTCATGCTTATCACCTTGGCCTGCGCTGCCATGACTGTCATCTTCTTCATCGTTAGTCAGGTAACGGAAGGCCATTG
GAAATGGGGCGGCGTCACAGTCCAAGTGAACAGTGCCTTTTTCACAGGCATCTATGGGATGTGGAATCTGTATGTCTTTGCTCTGATGTT
CTTGTATGCACCATCCCATAAAAACTATGGAGAAGACCAGTCCAATGGAATGCAACTCCCATGTAAATCGAGGGAAGATTGTGCTTTGTT
TGTTTCGGAACTTTATCAAGAATTGTTCAGCGCTTCGAAATATTCCTTCATCAATGACAACGCAGCTTCTGGTATTTGAGTCAACAAGGC
AACACATGTTTATCAGCTTTGCATTTGCAGTTGTCACAGTCACATTGATTGTACTTGTATACGCACACAAATACACTCATTTAGCCTTTA

>3245_3245_4_AK4-WLS_AK4_chr1_65614235_ENST00000327299_WLS_chr1_68624930_ENST00000540432_length(amino acids)=533AA_BP=108
MLLGPGAGLRGGVVLKVSPSPCRGGRRVPVRAEGARGRGPRNKVPGASSGAAVGAARLTAPLLAKAMASKLLRAVILGPPGSGKGTVCQR
IAQNFGLQHLSSGHFLRENIKASTGENAEVSMDVSLAYRDDAFAEWTEMAHERVPRKLKCTFTSPKTPEHEGRYYECDVLPFMEIGSVAH
KFYLLNIRLPVNEKKKINVGIGEIKDIRLVGIHQNGGFTKVWFAMKTFLTPSIFIIMVWYWRRITMMSRPPVLLEKVIFALGISMTFINI
PVEWFSIGFDWTWMLLFGDIRQGIFYAMLLSFWIIFCGEHMMDQHERNHIAGYWKQVGPIAVGSFCLFIFDMCERGVQLTNPFYSIWTTD
IGTELAMAFIIVAGICLCLYFLFLCFMVFQVFRNISGKQSSLPAMSKVRRLHYEGLIFRFKFLMLITLACAAMTVIFFIVSQVTEGHWKW

--------------------------------------------------------------
>3245_3245_5_AK4-WLS_AK4_chr1_65614235_ENST00000395334_WLS_chr1_68624930_ENST00000262348_length(transcript)=2618nt_BP=503nt
AGCCGCCGGGGAGGGGACGAGACGCAGACCACACGTTGCCCACGTGCGAGGACCGGGCAGGGGCTCAGCCCCTAGGCCGCCCGGTGCGTG
GGGCGGCGGGGGCTGGCTTTGAGTCACCCCCGCTTGTGCCAGCTTCCCCGGCTCGGCGGAGACTGGCTTGGGGATGCCTGGCCGCCCGGC
CCCCTTCTCGGACGCGGCGTGGGGAGCGAGCTGGAGTTAAGGATGAAGGGGCGGCCGGCGAGTCCCAGTGAGAGCGGAGGGTGCCAGAGG
TAGGGGGCCGAGAAACAAAGTTCCCGGGGCTTCCTCCGGGGCCGCGGTCGGGGCTGCGCGTTTGACCGCCCCCCTCCTCGCGAAGGCAAT
GGCTTCCAAACTCCTGCGCGCGGTCATCCTCGGGCCGCCCGGCTCGGGCAAGGGCACCGTGTGCCAGAGGATCGCCCAGAACTTTGGTCT
CCAGCATCTCTCCAGCGGCCACTTCTTGCGGGAGAACATCAAGGCCAGCACCGGAGAAAATGCAGAAGTCTCCATGGACGTTTCCCTGGC
TTACCGTGATGACGCGTTTGCTGAGTGGACTGAAATGGCCCATGAAAGAGTACCACGGAAACTCAAATGCACCTTCACATCTCCCAAGAC
TCCAGAGCATGAGGGCCGTTACTATGAATGTGATGTCCTTCCTTTCATGGAAATTGGGTCTGTGGCCCATAAGTTTTACCTTTTAAACAT
CCGGCTGCCTGTGAATGAGAAGAAGAAAATCAATGTGGGAATTGGGGAGATAAAGGATATCCGGTTGGTGGGGATCCACCAAAATGGAGG
CTTCACCAAGGTGTGGTTTGCCATGAAGACCTTCCTTACGCCCAGCATCTTCATCATTATGGTGTGGTATTGGAGGAGGATCACCATGAT
GTCCCGACCCCCAGTGCTTCTGGAAAAAGTCATCTTTGCCCTTGGGATTTCCATGACCTTTATCAATATCCCAGTGGAATGGTTTTCCAT
CGGGTTTGACTGGACCTGGATGCTGCTGTTTGGTGACATCCGACAGGGCATCTTCTATGCGATGCTTCTGTCCTTCTGGATCATCTTCTG
TGGCGAGCACATGATGGATCAGCACGAGCGGAACCACATCGCAGGGTATTGGAAGCAAGTCGGACCCATTGCCGTTGGCTCCTTCTGCCT
CTTCATATTTGACATGTGTGAGAGAGGGGTACAACTCACGAATCCCTTCTACAGTATCTGGACTACAGACATTGGAACAGAGCTGGCCAT
GGCCTTCATCATCGTGGCTGGAATCTGCCTCTGCCTCTACTTCCTGTTTCTATGCTTCATGGTATTTCAGGTGTTTCGGAACATCAGTGG
GAAGCAGTCCAGCCTGCCAGCTATGAGCAAAGTCCGGCGGCTACACTATGAGGGGCTAATTTTTAGGTTCAAGTTCCTCATGCTTATCAC
CTTGGCCTGCGCTGCCATGACTGTCATCTTCTTCATCGTTAGTCAGGTAACGGAAGGCCATTGGAAATGGGGCGGCGTCACAGTCCAAGT
GAACAGTGCCTTTTTCACAGGCATCTATGGGATGTGGAATCTGTATGTCTTTGCTCTGATGTTCTTGTATGCACCATCCCATAAAAACTA
TGGAGAAGACCAGTCCAATGGCGATCTGGGTGTCCATAGTGGGGAAGAACTCCAGCTCACCACCACTATCACCCATGTGGACGGACCCAC
TGAGATCTACAAGTTGACCCGCAAGGAGGCCCAGGAGTAGGAGGCTGCAGCGCCCGGCTGGGACGGTCTCTCCATACCCCAGCCCCTCTA
ACTAGAGTGGGGAGCATGCCAGAGAGAGCTCAATGTACAAATGAATGCCTCATGGCTCTTAGCTGTGGTTTCTTGGACCAGCGGCATGGA
CATTTGTCAGTTTGCCTTCTGACGGTAGCTTTTGGAGGAAGATTCCTGCAGCCACTAATGCATTGTGTATGATAACAAAAACTCTGGTAT
GACACATTTTCTGTGATCATTGTTAATTAGTGACATAGTAACATCTGTAGCAGCTGGTTAGTAAACCTCATGTGGGGGTGGGGTGGGGGT
GTATTCCTTGGGGGATGGTTTGGGCCGAATGGGGAGTGGAATATTTGACATTTTTCCTGTTTTAAATTCTAGGATAGATTTTAACATCCT
TTGCGGTCCCAGTCCAAGGTAGGCTGGTGTCATAGTCTTCTCACTCCTAATCCATGACCACTGTTTTTTTCCTATTTATATCACCAGGTA
GCCCACTGAGTTAATATTTAAGTTGTCAATAGATAAGTGTCCCTGTTTTGTGGCATAATATAACTGAATTTCATGAGAAGATTTATTCCA
CCAGGGGTATTTCAGCTTTGAAACCAAATCTGTGTATCTAATACTAACCAATCTGTTGGATGTGGGTTTTAAAAAATGTTTGCTAAACTA
CCCAAGTAAGATTTACTGTATTAAATGGCCTTCGGGTCTGAAAAGCTTTTTTAACTTCTTGCTTAAAATGCGTTTTATTTTGATAAGATA
CTTCAAATAGCCTCCAAAAGTGTAGATCCAATCATTTAAATAAACCTGTATGTATATGCATATGTGTACATGCATATCCCCTCCTGCTAA

>3245_3245_5_AK4-WLS_AK4_chr1_65614235_ENST00000395334_WLS_chr1_68624930_ENST00000262348_length(amino acids)=472AA_BP=51
MTAPLLAKAMASKLLRAVILGPPGSGKGTVCQRIAQNFGLQHLSSGHFLRENIKASTGENAEVSMDVSLAYRDDAFAEWTEMAHERVPRK
LKCTFTSPKTPEHEGRYYECDVLPFMEIGSVAHKFYLLNIRLPVNEKKKINVGIGEIKDIRLVGIHQNGGFTKVWFAMKTFLTPSIFIIM
VWYWRRITMMSRPPVLLEKVIFALGISMTFINIPVEWFSIGFDWTWMLLFGDIRQGIFYAMLLSFWIIFCGEHMMDQHERNHIAGYWKQV
GPIAVGSFCLFIFDMCERGVQLTNPFYSIWTTDIGTELAMAFIIVAGICLCLYFLFLCFMVFQVFRNISGKQSSLPAMSKVRRLHYEGLI
FRFKFLMLITLACAAMTVIFFIVSQVTEGHWKWGGVTVQVNSAFFTGIYGMWNLYVFALMFLYAPSHKNYGEDQSNGDLGVHSGEELQLT

--------------------------------------------------------------
>3245_3245_6_AK4-WLS_AK4_chr1_65614235_ENST00000395334_WLS_chr1_68624930_ENST00000354777_length(transcript)=1921nt_BP=503nt
AGCCGCCGGGGAGGGGACGAGACGCAGACCACACGTTGCCCACGTGCGAGGACCGGGCAGGGGCTCAGCCCCTAGGCCGCCCGGTGCGTG
GGGCGGCGGGGGCTGGCTTTGAGTCACCCCCGCTTGTGCCAGCTTCCCCGGCTCGGCGGAGACTGGCTTGGGGATGCCTGGCCGCCCGGC
CCCCTTCTCGGACGCGGCGTGGGGAGCGAGCTGGAGTTAAGGATGAAGGGGCGGCCGGCGAGTCCCAGTGAGAGCGGAGGGTGCCAGAGG
TAGGGGGCCGAGAAACAAAGTTCCCGGGGCTTCCTCCGGGGCCGCGGTCGGGGCTGCGCGTTTGACCGCCCCCCTCCTCGCGAAGGCAAT
GGCTTCCAAACTCCTGCGCGCGGTCATCCTCGGGCCGCCCGGCTCGGGCAAGGGCACCGTGTGCCAGAGGATCGCCCAGAACTTTGGTCT
CCAGCATCTCTCCAGCGGCCACTTCTTGCGGGAGAACATCAAGGCCAGCACCGGAGAAAATGCAGAAGTCTCCATGGACGTTTCCCTGGC
TTACCGTGATGACGCGTTTGCTGAGTGGACTGAAATGGCCCATGAAAGAGTACCACGGAAACTCAAATGCACCTTCACATCTCCCAAGAC
TCCAGAGCATGAGGGCCGTTACTATGAATGTGATGTCCTTCCTTTCATGGAAATTGGGTCTGTGGCCCATAAGTTTTACCTTTTAAACAT
CCGGCTGCCTGTGAATGAGAAGAAGAAAATCAATGTGGGAATTGGGGAGATAAAGGATATCCGGTTGGTGGGGATCCACCAAAATGGAGG
CTTCACCAAGGTGTGGTTTGCCATGAAGACCTTCCTTACGCCCAGCATCTTCATCATTATGGTGTGGTATTGGAGGAGGATCACCATGAT
GTCCCGACCCCCAGTGCTTCTGGAAAAAGTCATCTTTGCCCTTGGGATTTCCATGACCTTTATCAATATCCCAGTGGAATGGTTTTCCAT
CGGGTTTGACTGGACCTGGATGCTGCTGTTTGGTGACATCCGACAGGGCATCTTCTATGCGATGCTTCTGTCCTTCTGGATCATCTTCTG
TGGCGAGCACATGATGGATCAGCACGAGCGGAACCACATCGCAGGGTATTGGAAGCAAGTCGGACCCATTGCCGTTGGCTCCTTCTGCCT
CTTCATATTTGACATGTGTGAGAGAGGGGTACAACTCACGAATCCCTTCTACAGTATCTGGACTACAGACATTGGAACAGAGCTGGCCAT
GGCCTTCATCATCGTGGCTGGAATCTGCCTCTGCCTCTACTTCCTGTTTCTATGCTTCATGGTATTTCAGGTGTTTCGGAACATCAGTGG
GAAGCAGTCCAGCCTGCCAGCTATGAGCAAAGTCCGGCGGCTACACTATGAGGGGCTAATTTTTAGGTTCAAGTTCCTCATGCTTATCAC
CTTGGCCTGCGCTGCCATGACTGTCATCTTCTTCATCGTTAGTCAGGTAACGGAAGGCCATTGGAAATGGGGCGGCGTCACAGTCCAAGT
GAACAGTGCCTTTTTCACAGGCATCTATGGGATGTGGAATCTGTATGTCTTTGCTCTGATGTTCTTGTATGCACCATCCCATAAAAACTA
TGGAGAAGACCAGTCCAATGGAATGCAACTCCCATGTAAATCGAGGGAAGATTGTGCTTTGTTTGTTTCGGAACTTTATCAAGAATTGTT
CAGCGCTTCGAAATATTCCTTCATCAATGACAACGCAGCTTCTGGTATTTGAGTCAACAAGGCAACACATGTTTATCAGCTTTGCATTTG
CAGTTGTCACAGTCACATTGATTGTACTTGTATACGCACACAAATACACTCATTTAGCCTTTATCTCAAAATGTTAAATATAAGGAAAAA

>3245_3245_6_AK4-WLS_AK4_chr1_65614235_ENST00000395334_WLS_chr1_68624930_ENST00000354777_length(amino acids)=476AA_BP=51
MTAPLLAKAMASKLLRAVILGPPGSGKGTVCQRIAQNFGLQHLSSGHFLRENIKASTGENAEVSMDVSLAYRDDAFAEWTEMAHERVPRK
LKCTFTSPKTPEHEGRYYECDVLPFMEIGSVAHKFYLLNIRLPVNEKKKINVGIGEIKDIRLVGIHQNGGFTKVWFAMKTFLTPSIFIIM
VWYWRRITMMSRPPVLLEKVIFALGISMTFINIPVEWFSIGFDWTWMLLFGDIRQGIFYAMLLSFWIIFCGEHMMDQHERNHIAGYWKQV
GPIAVGSFCLFIFDMCERGVQLTNPFYSIWTTDIGTELAMAFIIVAGICLCLYFLFLCFMVFQVFRNISGKQSSLPAMSKVRRLHYEGLI
FRFKFLMLITLACAAMTVIFFIVSQVTEGHWKWGGVTVQVNSAFFTGIYGMWNLYVFALMFLYAPSHKNYGEDQSNGMQLPCKSREDCAL

--------------------------------------------------------------
>3245_3245_7_AK4-WLS_AK4_chr1_65614235_ENST00000395334_WLS_chr1_68624930_ENST00000370976_length(transcript)=2484nt_BP=503nt
AGCCGCCGGGGAGGGGACGAGACGCAGACCACACGTTGCCCACGTGCGAGGACCGGGCAGGGGCTCAGCCCCTAGGCCGCCCGGTGCGTG
GGGCGGCGGGGGCTGGCTTTGAGTCACCCCCGCTTGTGCCAGCTTCCCCGGCTCGGCGGAGACTGGCTTGGGGATGCCTGGCCGCCCGGC
CCCCTTCTCGGACGCGGCGTGGGGAGCGAGCTGGAGTTAAGGATGAAGGGGCGGCCGGCGAGTCCCAGTGAGAGCGGAGGGTGCCAGAGG
TAGGGGGCCGAGAAACAAAGTTCCCGGGGCTTCCTCCGGGGCCGCGGTCGGGGCTGCGCGTTTGACCGCCCCCCTCCTCGCGAAGGCAAT
GGCTTCCAAACTCCTGCGCGCGGTCATCCTCGGGCCGCCCGGCTCGGGCAAGGGCACCGTGTGCCAGAGGATCGCCCAGAACTTTGGTCT
CCAGCATCTCTCCAGCGGCCACTTCTTGCGGGAGAACATCAAGGCCAGCACCGGAGAAAATGCAGAAGTCTCCATGGACGTTTCCCTGGC
TTACCGTGATGACGCGTTTGCTGAGTGGACTGAAATGGCCCATGAAAGAGTACCACGGAAACTCAAATGCACCTTCACATCTCCCAAGAC
TCCAGAGCATGAGGGCCGTTACTATGAATGTGATGTCCTTCCTTTCATGGAAATTGGGTCTGTGGCCCATAAGTTTTACCTTTTAAACAT
CCGGCTGCCTGTGAATGAGAAGAAGAAAATCAATGTGGGAATTGGGGAGATAAAGGATATCCGGTTGGTGGGGATCCACCAAAATGGAGG
CTTCACCAAGGTGTGGTTTGCCATGAAGACCTTCCTTACGCCCAGCATCTTCATCATTATGGTGTGGTATTGGAGGAGGATCACCATGAT
GTCCCGACCCCCAGTGCTTCTGGAAAAAGTCATCTTTGCCCTTGGGATTTCCATGACCTTTATCAATATCCCAGTGGAATGGTTTTCCAT
CGGGTTTGACTGGACCTGGATGCTGCTGTTTGGTGACATCCGACAGGGCATCTTCTATGCGATGCTTCTGTCCTTCTGGATCATCTTCTG
TGGCGAGCACATGATGGATCAGCACGAGCGGAACCACATCGCAGGGTATTGGAAGCAAGTCGGACCCATTGCCGTTGGCTCCTTCTGCCT
CTTCATATTTGACATGTGTGAGAGAGGGGTACAACTCACGAATCCCTTCTACAGTATCTGGACTACAGACATTGGAACAGAGCTGGCCAT
GGCCTTCATCATCGTGGCTGGAATCTGCCTCTGCCTCTACTTCCTGTTTCTATGCTTCATGGTATTTCAGGTGTTTCGGAACATCAGTGG
GAAGCAGTCCAGCCTGCCAGCTATGAGCAAAGTCCGGCGGCTACACTATGAGGGGCTAATTTTTAGGTTCAAGTTCCTCATGCTTATCAC
CTTGGCCTGCGCTGCCATGACTGTCATCTTCTTCATCGTTAGTCAGGTAACGGAAGGCCATTGGAAATGGGGCGGCGTCACAGTCCAAGT
GAACAGTGCCTTTTTCACAGGCATCTATGGGATGTGGAATCTGTATGTCTTTGCTCTGATGTTCTTGTATGCACCATCCCATAAAAACTA
TGGAGAAGACCAGTCCAATGGCGATCTGGGTGTCCATAGTGGGGAAGAACTCCAGCTCACCACCACTATCACCCATGTGGACGGACCCAC
TGAGATCTACAAGTTGACCCGCAAGGAGGCCCAGGAGTAGGAGGCTGCAGCGCCCGGCTGGGACGGTCTCTCCATACCCCAGCCCCTCTA
ACTAGAGTGGGGAGCATGCCAGAGAGAGCTCAATGTACAAATGAATGCCTCATGGCTCTTAGCTGTGGTTTCTTGGACCAGCGGCATGGA
CATTTGTCAGTTTGCCTTCTGACGGTAGCTTTTGGAGGAAGATTCCTGCAGCCACTAATGCATTGTGTATGATAACAAAAACTCTGGTAT
GACACATTTTCTGTGATCATTGTTAATTAGTGACATAGTAACATCTGTAGCAGCTGGTTAGTAAACCTCATGTGGGGGTGGGGTGGGGGT
GTATTCCTTGGGGGATGGTTTGGGCCGAATGGGGAGTGGAATATTTGACATTTTTCCTGTTTTAAATTCTAGGATAGATTTTAACATCCT
TTGCGGTCCCAGTCCAAGGTAGGCTGGTGTCATAGTCTTCTCACTCCTAATCCATGACCACTGTTTTTTTCCTATTTATATCACCAGGTA
GCCCACTGAGTTAATATTTAAGTTGTCAATAGATAAGTGTCCCTGTTTTGTGGCATAATATAACTGAATTTCATGAGAAGATTTATTCCA
CCAGGGGTATTTCAGCTTTGAAACCAAATCTGTGTATCTAATACTAACCAATCTGTTGGATGTGGGTTTTAAAAAATGTTTGCTAAACTA

>3245_3245_7_AK4-WLS_AK4_chr1_65614235_ENST00000395334_WLS_chr1_68624930_ENST00000370976_length(amino acids)=472AA_BP=51
MTAPLLAKAMASKLLRAVILGPPGSGKGTVCQRIAQNFGLQHLSSGHFLRENIKASTGENAEVSMDVSLAYRDDAFAEWTEMAHERVPRK
LKCTFTSPKTPEHEGRYYECDVLPFMEIGSVAHKFYLLNIRLPVNEKKKINVGIGEIKDIRLVGIHQNGGFTKVWFAMKTFLTPSIFIIM
VWYWRRITMMSRPPVLLEKVIFALGISMTFINIPVEWFSIGFDWTWMLLFGDIRQGIFYAMLLSFWIIFCGEHMMDQHERNHIAGYWKQV
GPIAVGSFCLFIFDMCERGVQLTNPFYSIWTTDIGTELAMAFIIVAGICLCLYFLFLCFMVFQVFRNISGKQSSLPAMSKVRRLHYEGLI
FRFKFLMLITLACAAMTVIFFIVSQVTEGHWKWGGVTVQVNSAFFTGIYGMWNLYVFALMFLYAPSHKNYGEDQSNGDLGVHSGEELQLT

--------------------------------------------------------------
>3245_3245_8_AK4-WLS_AK4_chr1_65614235_ENST00000395334_WLS_chr1_68624930_ENST00000540432_length(transcript)=1935nt_BP=503nt
AGCCGCCGGGGAGGGGACGAGACGCAGACCACACGTTGCCCACGTGCGAGGACCGGGCAGGGGCTCAGCCCCTAGGCCGCCCGGTGCGTG
GGGCGGCGGGGGCTGGCTTTGAGTCACCCCCGCTTGTGCCAGCTTCCCCGGCTCGGCGGAGACTGGCTTGGGGATGCCTGGCCGCCCGGC
CCCCTTCTCGGACGCGGCGTGGGGAGCGAGCTGGAGTTAAGGATGAAGGGGCGGCCGGCGAGTCCCAGTGAGAGCGGAGGGTGCCAGAGG
TAGGGGGCCGAGAAACAAAGTTCCCGGGGCTTCCTCCGGGGCCGCGGTCGGGGCTGCGCGTTTGACCGCCCCCCTCCTCGCGAAGGCAAT
GGCTTCCAAACTCCTGCGCGCGGTCATCCTCGGGCCGCCCGGCTCGGGCAAGGGCACCGTGTGCCAGAGGATCGCCCAGAACTTTGGTCT
CCAGCATCTCTCCAGCGGCCACTTCTTGCGGGAGAACATCAAGGCCAGCACCGGAGAAAATGCAGAAGTCTCCATGGACGTTTCCCTGGC
TTACCGTGATGACGCGTTTGCTGAGTGGACTGAAATGGCCCATGAAAGAGTACCACGGAAACTCAAATGCACCTTCACATCTCCCAAGAC
TCCAGAGCATGAGGGCCGTTACTATGAATGTGATGTCCTTCCTTTCATGGAAATTGGGTCTGTGGCCCATAAGTTTTACCTTTTAAACAT
CCGGCTGCCTGTGAATGAGAAGAAGAAAATCAATGTGGGAATTGGGGAGATAAAGGATATCCGGTTGGTGGGGATCCACCAAAATGGAGG
CTTCACCAAGGTGTGGTTTGCCATGAAGACCTTCCTTACGCCCAGCATCTTCATCATTATGGTGTGGTATTGGAGGAGGATCACCATGAT
GTCCCGACCCCCAGTGCTTCTGGAAAAAGTCATCTTTGCCCTTGGGATTTCCATGACCTTTATCAATATCCCAGTGGAATGGTTTTCCAT
CGGGTTTGACTGGACCTGGATGCTGCTGTTTGGTGACATCCGACAGGGCATCTTCTATGCGATGCTTCTGTCCTTCTGGATCATCTTCTG
TGGCGAGCACATGATGGATCAGCACGAGCGGAACCACATCGCAGGGTATTGGAAGCAAGTCGGACCCATTGCCGTTGGCTCCTTCTGCCT
CTTCATATTTGACATGTGTGAGAGAGGGGTACAACTCACGAATCCCTTCTACAGTATCTGGACTACAGACATTGGAACAGAGCTGGCCAT
GGCCTTCATCATCGTGGCTGGAATCTGCCTCTGCCTCTACTTCCTGTTTCTATGCTTCATGGTATTTCAGGTGTTTCGGAACATCAGTGG
GAAGCAGTCCAGCCTGCCAGCTATGAGCAAAGTCCGGCGGCTACACTATGAGGGGCTAATTTTTAGGTTCAAGTTCCTCATGCTTATCAC
CTTGGCCTGCGCTGCCATGACTGTCATCTTCTTCATCGTTAGTCAGGTAACGGAAGGCCATTGGAAATGGGGCGGCGTCACAGTCCAAGT
GAACAGTGCCTTTTTCACAGGCATCTATGGGATGTGGAATCTGTATGTCTTTGCTCTGATGTTCTTGTATGCACCATCCCATAAAAACTA
TGGAGAAGACCAGTCCAATGGAATGCAACTCCCATGTAAATCGAGGGAAGATTGTGCTTTGTTTGTTTCGGAACTTTATCAAGAATTGTT
CAGCGCTTCGAAATATTCCTTCATCAATGACAACGCAGCTTCTGGTATTTGAGTCAACAAGGCAACACATGTTTATCAGCTTTGCATTTG
CAGTTGTCACAGTCACATTGATTGTACTTGTATACGCACACAAATACACTCATTTAGCCTTTATCTCAAAATGTTAAATATAAGGAAAAA

>3245_3245_8_AK4-WLS_AK4_chr1_65614235_ENST00000395334_WLS_chr1_68624930_ENST00000540432_length(amino acids)=476AA_BP=51
MTAPLLAKAMASKLLRAVILGPPGSGKGTVCQRIAQNFGLQHLSSGHFLRENIKASTGENAEVSMDVSLAYRDDAFAEWTEMAHERVPRK
LKCTFTSPKTPEHEGRYYECDVLPFMEIGSVAHKFYLLNIRLPVNEKKKINVGIGEIKDIRLVGIHQNGGFTKVWFAMKTFLTPSIFIIM
VWYWRRITMMSRPPVLLEKVIFALGISMTFINIPVEWFSIGFDWTWMLLFGDIRQGIFYAMLLSFWIIFCGEHMMDQHERNHIAGYWKQV
GPIAVGSFCLFIFDMCERGVQLTNPFYSIWTTDIGTELAMAFIIVAGICLCLYFLFLCFMVFQVFRNISGKQSSLPAMSKVRRLHYEGLI
FRFKFLMLITLACAAMTVIFFIVSQVTEGHWKWGGVTVQVNSAFFTGIYGMWNLYVFALMFLYAPSHKNYGEDQSNGMQLPCKSREDCAL

--------------------------------------------------------------
>3245_3245_9_AK4-WLS_AK4_chr1_65614235_ENST00000545314_WLS_chr1_68624930_ENST00000262348_length(transcript)=2507nt_BP=392nt
TTACGTAAGGCGCGAGTCCCGCGCGGCCGGCCGGGGCTCCGCGACTGGGCGGGCGGAGGCGGCGCCGCCCTGCAGGATCCGGGCTGGAAG
CCTCTCGGAGTGCTGCCTCCAGCCAAGGGGCGGCCGGCGAGTCCCAGTGAGAGCGGAGGGTGCCAGAGGTAGGGGGCCGAGAAACAAAGT
TCCCGGGGCTTCCTCCGGGGCCGCGGTCGGGGCTGCGCGTTTGACCGCCCCCCTCCTCGCGAAGGCAATGGCTTCCAAACTCCTGCGCGC
GGTCATCCTCGGGCCGCCCGGCTCGGGCAAGGGCACCGTGTGCCAGAGGATCGCCCAGAACTTTGGTCTCCAGCATCTCTCCAGCGGCCA
CTTCTTGCGGGAGAACATCAAGGCCAGCACCGGAGAAAATGCAGAAGTCTCCATGGACGTTTCCCTGGCTTACCGTGATGACGCGTTTGC
TGAGTGGACTGAAATGGCCCATGAAAGAGTACCACGGAAACTCAAATGCACCTTCACATCTCCCAAGACTCCAGAGCATGAGGGCCGTTA
CTATGAATGTGATGTCCTTCCTTTCATGGAAATTGGGTCTGTGGCCCATAAGTTTTACCTTTTAAACATCCGGCTGCCTGTGAATGAGAA
GAAGAAAATCAATGTGGGAATTGGGGAGATAAAGGATATCCGGTTGGTGGGGATCCACCAAAATGGAGGCTTCACCAAGGTGTGGTTTGC
CATGAAGACCTTCCTTACGCCCAGCATCTTCATCATTATGGTGTGGTATTGGAGGAGGATCACCATGATGTCCCGACCCCCAGTGCTTCT
GGAAAAAGTCATCTTTGCCCTTGGGATTTCCATGACCTTTATCAATATCCCAGTGGAATGGTTTTCCATCGGGTTTGACTGGACCTGGAT
GCTGCTGTTTGGTGACATCCGACAGGGCATCTTCTATGCGATGCTTCTGTCCTTCTGGATCATCTTCTGTGGCGAGCACATGATGGATCA
GCACGAGCGGAACCACATCGCAGGGTATTGGAAGCAAGTCGGACCCATTGCCGTTGGCTCCTTCTGCCTCTTCATATTTGACATGTGTGA
GAGAGGGGTACAACTCACGAATCCCTTCTACAGTATCTGGACTACAGACATTGGAACAGAGCTGGCCATGGCCTTCATCATCGTGGCTGG
AATCTGCCTCTGCCTCTACTTCCTGTTTCTATGCTTCATGGTATTTCAGGTGTTTCGGAACATCAGTGGGAAGCAGTCCAGCCTGCCAGC
TATGAGCAAAGTCCGGCGGCTACACTATGAGGGGCTAATTTTTAGGTTCAAGTTCCTCATGCTTATCACCTTGGCCTGCGCTGCCATGAC
TGTCATCTTCTTCATCGTTAGTCAGGTAACGGAAGGCCATTGGAAATGGGGCGGCGTCACAGTCCAAGTGAACAGTGCCTTTTTCACAGG
CATCTATGGGATGTGGAATCTGTATGTCTTTGCTCTGATGTTCTTGTATGCACCATCCCATAAAAACTATGGAGAAGACCAGTCCAATGG
CGATCTGGGTGTCCATAGTGGGGAAGAACTCCAGCTCACCACCACTATCACCCATGTGGACGGACCCACTGAGATCTACAAGTTGACCCG
CAAGGAGGCCCAGGAGTAGGAGGCTGCAGCGCCCGGCTGGGACGGTCTCTCCATACCCCAGCCCCTCTAACTAGAGTGGGGAGCATGCCA
GAGAGAGCTCAATGTACAAATGAATGCCTCATGGCTCTTAGCTGTGGTTTCTTGGACCAGCGGCATGGACATTTGTCAGTTTGCCTTCTG
ACGGTAGCTTTTGGAGGAAGATTCCTGCAGCCACTAATGCATTGTGTATGATAACAAAAACTCTGGTATGACACATTTTCTGTGATCATT
GTTAATTAGTGACATAGTAACATCTGTAGCAGCTGGTTAGTAAACCTCATGTGGGGGTGGGGTGGGGGTGTATTCCTTGGGGGATGGTTT
GGGCCGAATGGGGAGTGGAATATTTGACATTTTTCCTGTTTTAAATTCTAGGATAGATTTTAACATCCTTTGCGGTCCCAGTCCAAGGTA
GGCTGGTGTCATAGTCTTCTCACTCCTAATCCATGACCACTGTTTTTTTCCTATTTATATCACCAGGTAGCCCACTGAGTTAATATTTAA
GTTGTCAATAGATAAGTGTCCCTGTTTTGTGGCATAATATAACTGAATTTCATGAGAAGATTTATTCCACCAGGGGTATTTCAGCTTTGA
AACCAAATCTGTGTATCTAATACTAACCAATCTGTTGGATGTGGGTTTTAAAAAATGTTTGCTAAACTACCCAAGTAAGATTTACTGTAT
TAAATGGCCTTCGGGTCTGAAAAGCTTTTTTAACTTCTTGCTTAAAATGCGTTTTATTTTGATAAGATACTTCAAATAGCCTCCAAAAGT

>3245_3245_9_AK4-WLS_AK4_chr1_65614235_ENST00000545314_WLS_chr1_68624930_ENST00000262348_length(amino acids)=472AA_BP=51
MTAPLLAKAMASKLLRAVILGPPGSGKGTVCQRIAQNFGLQHLSSGHFLRENIKASTGENAEVSMDVSLAYRDDAFAEWTEMAHERVPRK
LKCTFTSPKTPEHEGRYYECDVLPFMEIGSVAHKFYLLNIRLPVNEKKKINVGIGEIKDIRLVGIHQNGGFTKVWFAMKTFLTPSIFIIM
VWYWRRITMMSRPPVLLEKVIFALGISMTFINIPVEWFSIGFDWTWMLLFGDIRQGIFYAMLLSFWIIFCGEHMMDQHERNHIAGYWKQV
GPIAVGSFCLFIFDMCERGVQLTNPFYSIWTTDIGTELAMAFIIVAGICLCLYFLFLCFMVFQVFRNISGKQSSLPAMSKVRRLHYEGLI
FRFKFLMLITLACAAMTVIFFIVSQVTEGHWKWGGVTVQVNSAFFTGIYGMWNLYVFALMFLYAPSHKNYGEDQSNGDLGVHSGEELQLT

--------------------------------------------------------------
>3245_3245_10_AK4-WLS_AK4_chr1_65614235_ENST00000545314_WLS_chr1_68624930_ENST00000354777_length(transcript)=1810nt_BP=392nt
TTACGTAAGGCGCGAGTCCCGCGCGGCCGGCCGGGGCTCCGCGACTGGGCGGGCGGAGGCGGCGCCGCCCTGCAGGATCCGGGCTGGAAG
CCTCTCGGAGTGCTGCCTCCAGCCAAGGGGCGGCCGGCGAGTCCCAGTGAGAGCGGAGGGTGCCAGAGGTAGGGGGCCGAGAAACAAAGT
TCCCGGGGCTTCCTCCGGGGCCGCGGTCGGGGCTGCGCGTTTGACCGCCCCCCTCCTCGCGAAGGCAATGGCTTCCAAACTCCTGCGCGC
GGTCATCCTCGGGCCGCCCGGCTCGGGCAAGGGCACCGTGTGCCAGAGGATCGCCCAGAACTTTGGTCTCCAGCATCTCTCCAGCGGCCA
CTTCTTGCGGGAGAACATCAAGGCCAGCACCGGAGAAAATGCAGAAGTCTCCATGGACGTTTCCCTGGCTTACCGTGATGACGCGTTTGC
TGAGTGGACTGAAATGGCCCATGAAAGAGTACCACGGAAACTCAAATGCACCTTCACATCTCCCAAGACTCCAGAGCATGAGGGCCGTTA
CTATGAATGTGATGTCCTTCCTTTCATGGAAATTGGGTCTGTGGCCCATAAGTTTTACCTTTTAAACATCCGGCTGCCTGTGAATGAGAA
GAAGAAAATCAATGTGGGAATTGGGGAGATAAAGGATATCCGGTTGGTGGGGATCCACCAAAATGGAGGCTTCACCAAGGTGTGGTTTGC
CATGAAGACCTTCCTTACGCCCAGCATCTTCATCATTATGGTGTGGTATTGGAGGAGGATCACCATGATGTCCCGACCCCCAGTGCTTCT
GGAAAAAGTCATCTTTGCCCTTGGGATTTCCATGACCTTTATCAATATCCCAGTGGAATGGTTTTCCATCGGGTTTGACTGGACCTGGAT
GCTGCTGTTTGGTGACATCCGACAGGGCATCTTCTATGCGATGCTTCTGTCCTTCTGGATCATCTTCTGTGGCGAGCACATGATGGATCA
GCACGAGCGGAACCACATCGCAGGGTATTGGAAGCAAGTCGGACCCATTGCCGTTGGCTCCTTCTGCCTCTTCATATTTGACATGTGTGA
GAGAGGGGTACAACTCACGAATCCCTTCTACAGTATCTGGACTACAGACATTGGAACAGAGCTGGCCATGGCCTTCATCATCGTGGCTGG
AATCTGCCTCTGCCTCTACTTCCTGTTTCTATGCTTCATGGTATTTCAGGTGTTTCGGAACATCAGTGGGAAGCAGTCCAGCCTGCCAGC
TATGAGCAAAGTCCGGCGGCTACACTATGAGGGGCTAATTTTTAGGTTCAAGTTCCTCATGCTTATCACCTTGGCCTGCGCTGCCATGAC
TGTCATCTTCTTCATCGTTAGTCAGGTAACGGAAGGCCATTGGAAATGGGGCGGCGTCACAGTCCAAGTGAACAGTGCCTTTTTCACAGG
CATCTATGGGATGTGGAATCTGTATGTCTTTGCTCTGATGTTCTTGTATGCACCATCCCATAAAAACTATGGAGAAGACCAGTCCAATGG
AATGCAACTCCCATGTAAATCGAGGGAAGATTGTGCTTTGTTTGTTTCGGAACTTTATCAAGAATTGTTCAGCGCTTCGAAATATTCCTT
CATCAATGACAACGCAGCTTCTGGTATTTGAGTCAACAAGGCAACACATGTTTATCAGCTTTGCATTTGCAGTTGTCACAGTCACATTGA
TTGTACTTGTATACGCACACAAATACACTCATTTAGCCTTTATCTCAAAATGTTAAATATAAGGAAAAAAGCGTCAACAATAAATATTCT

>3245_3245_10_AK4-WLS_AK4_chr1_65614235_ENST00000545314_WLS_chr1_68624930_ENST00000354777_length(amino acids)=476AA_BP=51
MTAPLLAKAMASKLLRAVILGPPGSGKGTVCQRIAQNFGLQHLSSGHFLRENIKASTGENAEVSMDVSLAYRDDAFAEWTEMAHERVPRK
LKCTFTSPKTPEHEGRYYECDVLPFMEIGSVAHKFYLLNIRLPVNEKKKINVGIGEIKDIRLVGIHQNGGFTKVWFAMKTFLTPSIFIIM
VWYWRRITMMSRPPVLLEKVIFALGISMTFINIPVEWFSIGFDWTWMLLFGDIRQGIFYAMLLSFWIIFCGEHMMDQHERNHIAGYWKQV
GPIAVGSFCLFIFDMCERGVQLTNPFYSIWTTDIGTELAMAFIIVAGICLCLYFLFLCFMVFQVFRNISGKQSSLPAMSKVRRLHYEGLI
FRFKFLMLITLACAAMTVIFFIVSQVTEGHWKWGGVTVQVNSAFFTGIYGMWNLYVFALMFLYAPSHKNYGEDQSNGMQLPCKSREDCAL

--------------------------------------------------------------
>3245_3245_11_AK4-WLS_AK4_chr1_65614235_ENST00000545314_WLS_chr1_68624930_ENST00000370976_length(transcript)=2373nt_BP=392nt
TTACGTAAGGCGCGAGTCCCGCGCGGCCGGCCGGGGCTCCGCGACTGGGCGGGCGGAGGCGGCGCCGCCCTGCAGGATCCGGGCTGGAAG
CCTCTCGGAGTGCTGCCTCCAGCCAAGGGGCGGCCGGCGAGTCCCAGTGAGAGCGGAGGGTGCCAGAGGTAGGGGGCCGAGAAACAAAGT
TCCCGGGGCTTCCTCCGGGGCCGCGGTCGGGGCTGCGCGTTTGACCGCCCCCCTCCTCGCGAAGGCAATGGCTTCCAAACTCCTGCGCGC
GGTCATCCTCGGGCCGCCCGGCTCGGGCAAGGGCACCGTGTGCCAGAGGATCGCCCAGAACTTTGGTCTCCAGCATCTCTCCAGCGGCCA
CTTCTTGCGGGAGAACATCAAGGCCAGCACCGGAGAAAATGCAGAAGTCTCCATGGACGTTTCCCTGGCTTACCGTGATGACGCGTTTGC
TGAGTGGACTGAAATGGCCCATGAAAGAGTACCACGGAAACTCAAATGCACCTTCACATCTCCCAAGACTCCAGAGCATGAGGGCCGTTA
CTATGAATGTGATGTCCTTCCTTTCATGGAAATTGGGTCTGTGGCCCATAAGTTTTACCTTTTAAACATCCGGCTGCCTGTGAATGAGAA
GAAGAAAATCAATGTGGGAATTGGGGAGATAAAGGATATCCGGTTGGTGGGGATCCACCAAAATGGAGGCTTCACCAAGGTGTGGTTTGC
CATGAAGACCTTCCTTACGCCCAGCATCTTCATCATTATGGTGTGGTATTGGAGGAGGATCACCATGATGTCCCGACCCCCAGTGCTTCT
GGAAAAAGTCATCTTTGCCCTTGGGATTTCCATGACCTTTATCAATATCCCAGTGGAATGGTTTTCCATCGGGTTTGACTGGACCTGGAT
GCTGCTGTTTGGTGACATCCGACAGGGCATCTTCTATGCGATGCTTCTGTCCTTCTGGATCATCTTCTGTGGCGAGCACATGATGGATCA
GCACGAGCGGAACCACATCGCAGGGTATTGGAAGCAAGTCGGACCCATTGCCGTTGGCTCCTTCTGCCTCTTCATATTTGACATGTGTGA
GAGAGGGGTACAACTCACGAATCCCTTCTACAGTATCTGGACTACAGACATTGGAACAGAGCTGGCCATGGCCTTCATCATCGTGGCTGG
AATCTGCCTCTGCCTCTACTTCCTGTTTCTATGCTTCATGGTATTTCAGGTGTTTCGGAACATCAGTGGGAAGCAGTCCAGCCTGCCAGC
TATGAGCAAAGTCCGGCGGCTACACTATGAGGGGCTAATTTTTAGGTTCAAGTTCCTCATGCTTATCACCTTGGCCTGCGCTGCCATGAC
TGTCATCTTCTTCATCGTTAGTCAGGTAACGGAAGGCCATTGGAAATGGGGCGGCGTCACAGTCCAAGTGAACAGTGCCTTTTTCACAGG
CATCTATGGGATGTGGAATCTGTATGTCTTTGCTCTGATGTTCTTGTATGCACCATCCCATAAAAACTATGGAGAAGACCAGTCCAATGG
CGATCTGGGTGTCCATAGTGGGGAAGAACTCCAGCTCACCACCACTATCACCCATGTGGACGGACCCACTGAGATCTACAAGTTGACCCG
CAAGGAGGCCCAGGAGTAGGAGGCTGCAGCGCCCGGCTGGGACGGTCTCTCCATACCCCAGCCCCTCTAACTAGAGTGGGGAGCATGCCA
GAGAGAGCTCAATGTACAAATGAATGCCTCATGGCTCTTAGCTGTGGTTTCTTGGACCAGCGGCATGGACATTTGTCAGTTTGCCTTCTG
ACGGTAGCTTTTGGAGGAAGATTCCTGCAGCCACTAATGCATTGTGTATGATAACAAAAACTCTGGTATGACACATTTTCTGTGATCATT
GTTAATTAGTGACATAGTAACATCTGTAGCAGCTGGTTAGTAAACCTCATGTGGGGGTGGGGTGGGGGTGTATTCCTTGGGGGATGGTTT
GGGCCGAATGGGGAGTGGAATATTTGACATTTTTCCTGTTTTAAATTCTAGGATAGATTTTAACATCCTTTGCGGTCCCAGTCCAAGGTA
GGCTGGTGTCATAGTCTTCTCACTCCTAATCCATGACCACTGTTTTTTTCCTATTTATATCACCAGGTAGCCCACTGAGTTAATATTTAA
GTTGTCAATAGATAAGTGTCCCTGTTTTGTGGCATAATATAACTGAATTTCATGAGAAGATTTATTCCACCAGGGGTATTTCAGCTTTGA
AACCAAATCTGTGTATCTAATACTAACCAATCTGTTGGATGTGGGTTTTAAAAAATGTTTGCTAAACTACCCAAGTAAGATTTACTGTAT

>3245_3245_11_AK4-WLS_AK4_chr1_65614235_ENST00000545314_WLS_chr1_68624930_ENST00000370976_length(amino acids)=472AA_BP=51
MTAPLLAKAMASKLLRAVILGPPGSGKGTVCQRIAQNFGLQHLSSGHFLRENIKASTGENAEVSMDVSLAYRDDAFAEWTEMAHERVPRK
LKCTFTSPKTPEHEGRYYECDVLPFMEIGSVAHKFYLLNIRLPVNEKKKINVGIGEIKDIRLVGIHQNGGFTKVWFAMKTFLTPSIFIIM
VWYWRRITMMSRPPVLLEKVIFALGISMTFINIPVEWFSIGFDWTWMLLFGDIRQGIFYAMLLSFWIIFCGEHMMDQHERNHIAGYWKQV
GPIAVGSFCLFIFDMCERGVQLTNPFYSIWTTDIGTELAMAFIIVAGICLCLYFLFLCFMVFQVFRNISGKQSSLPAMSKVRRLHYEGLI
FRFKFLMLITLACAAMTVIFFIVSQVTEGHWKWGGVTVQVNSAFFTGIYGMWNLYVFALMFLYAPSHKNYGEDQSNGDLGVHSGEELQLT

--------------------------------------------------------------
>3245_3245_12_AK4-WLS_AK4_chr1_65614235_ENST00000545314_WLS_chr1_68624930_ENST00000540432_length(transcript)=1824nt_BP=392nt
TTACGTAAGGCGCGAGTCCCGCGCGGCCGGCCGGGGCTCCGCGACTGGGCGGGCGGAGGCGGCGCCGCCCTGCAGGATCCGGGCTGGAAG
CCTCTCGGAGTGCTGCCTCCAGCCAAGGGGCGGCCGGCGAGTCCCAGTGAGAGCGGAGGGTGCCAGAGGTAGGGGGCCGAGAAACAAAGT
TCCCGGGGCTTCCTCCGGGGCCGCGGTCGGGGCTGCGCGTTTGACCGCCCCCCTCCTCGCGAAGGCAATGGCTTCCAAACTCCTGCGCGC
GGTCATCCTCGGGCCGCCCGGCTCGGGCAAGGGCACCGTGTGCCAGAGGATCGCCCAGAACTTTGGTCTCCAGCATCTCTCCAGCGGCCA
CTTCTTGCGGGAGAACATCAAGGCCAGCACCGGAGAAAATGCAGAAGTCTCCATGGACGTTTCCCTGGCTTACCGTGATGACGCGTTTGC
TGAGTGGACTGAAATGGCCCATGAAAGAGTACCACGGAAACTCAAATGCACCTTCACATCTCCCAAGACTCCAGAGCATGAGGGCCGTTA
CTATGAATGTGATGTCCTTCCTTTCATGGAAATTGGGTCTGTGGCCCATAAGTTTTACCTTTTAAACATCCGGCTGCCTGTGAATGAGAA
GAAGAAAATCAATGTGGGAATTGGGGAGATAAAGGATATCCGGTTGGTGGGGATCCACCAAAATGGAGGCTTCACCAAGGTGTGGTTTGC
CATGAAGACCTTCCTTACGCCCAGCATCTTCATCATTATGGTGTGGTATTGGAGGAGGATCACCATGATGTCCCGACCCCCAGTGCTTCT
GGAAAAAGTCATCTTTGCCCTTGGGATTTCCATGACCTTTATCAATATCCCAGTGGAATGGTTTTCCATCGGGTTTGACTGGACCTGGAT
GCTGCTGTTTGGTGACATCCGACAGGGCATCTTCTATGCGATGCTTCTGTCCTTCTGGATCATCTTCTGTGGCGAGCACATGATGGATCA
GCACGAGCGGAACCACATCGCAGGGTATTGGAAGCAAGTCGGACCCATTGCCGTTGGCTCCTTCTGCCTCTTCATATTTGACATGTGTGA
GAGAGGGGTACAACTCACGAATCCCTTCTACAGTATCTGGACTACAGACATTGGAACAGAGCTGGCCATGGCCTTCATCATCGTGGCTGG
AATCTGCCTCTGCCTCTACTTCCTGTTTCTATGCTTCATGGTATTTCAGGTGTTTCGGAACATCAGTGGGAAGCAGTCCAGCCTGCCAGC
TATGAGCAAAGTCCGGCGGCTACACTATGAGGGGCTAATTTTTAGGTTCAAGTTCCTCATGCTTATCACCTTGGCCTGCGCTGCCATGAC
TGTCATCTTCTTCATCGTTAGTCAGGTAACGGAAGGCCATTGGAAATGGGGCGGCGTCACAGTCCAAGTGAACAGTGCCTTTTTCACAGG
CATCTATGGGATGTGGAATCTGTATGTCTTTGCTCTGATGTTCTTGTATGCACCATCCCATAAAAACTATGGAGAAGACCAGTCCAATGG
AATGCAACTCCCATGTAAATCGAGGGAAGATTGTGCTTTGTTTGTTTCGGAACTTTATCAAGAATTGTTCAGCGCTTCGAAATATTCCTT
CATCAATGACAACGCAGCTTCTGGTATTTGAGTCAACAAGGCAACACATGTTTATCAGCTTTGCATTTGCAGTTGTCACAGTCACATTGA
TTGTACTTGTATACGCACACAAATACACTCATTTAGCCTTTATCTCAAAATGTTAAATATAAGGAAAAAAGCGTCAACAATAAATATTCT

>3245_3245_12_AK4-WLS_AK4_chr1_65614235_ENST00000545314_WLS_chr1_68624930_ENST00000540432_length(amino acids)=476AA_BP=51
MTAPLLAKAMASKLLRAVILGPPGSGKGTVCQRIAQNFGLQHLSSGHFLRENIKASTGENAEVSMDVSLAYRDDAFAEWTEMAHERVPRK
LKCTFTSPKTPEHEGRYYECDVLPFMEIGSVAHKFYLLNIRLPVNEKKKINVGIGEIKDIRLVGIHQNGGFTKVWFAMKTFLTPSIFIIM
VWYWRRITMMSRPPVLLEKVIFALGISMTFINIPVEWFSIGFDWTWMLLFGDIRQGIFYAMLLSFWIIFCGEHMMDQHERNHIAGYWKQV
GPIAVGSFCLFIFDMCERGVQLTNPFYSIWTTDIGTELAMAFIIVAGICLCLYFLFLCFMVFQVFRNISGKQSSLPAMSKVRRLHYEGLI
FRFKFLMLITLACAAMTVIFFIVSQVTEGHWKWGGVTVQVNSAFFTGIYGMWNLYVFALMFLYAPSHKNYGEDQSNGMQLPCKSREDCAL

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Fusion Gene PPI Analysis for AK4-WLS


check button Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in

ChiPPI page.


check button Protein-protein interactors with each fusion partner protein in wild-type (BIOGRID-3.4.160)
HgeneHgene's interactorsTgeneTgene's interactors


check button - Retained PPIs in in-frame fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenStill interaction with
TgeneWLSchr1:65614235chr1:68624930ENST00000370976011101_23235.333333333333336451.0Wnt proteins


check button - Lost PPIs in in-frame fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenInteraction lost with
TgeneWLSchr1:65614235chr1:68624930ENST00000262348112101_232126.33333333333333542.0Wnt proteins
TgeneWLSchr1:65614235chr1:68624930ENST00000354777112101_232124.33333333333333544.0Wnt proteins


check button - Retained PPIs, but lost function due to frame-shift fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenInteraction lost with


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Related Drugs for AK4-WLS


check button Drugs targeting genes involved in this fusion gene.
(DrugBank Version 5.1.8 2021-05-08)
PartnerGeneUniProtAccDrugBank IDDrug nameDrug activityDrug typeDrug status

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Related Diseases for AK4-WLS


check button Diseases associated with fusion partners.
(DisGeNet 4.0)
PartnerGeneDisease IDDisease name# pubmedsSource
HgeneAK4C0029408Degenerative polyarthritis1CTD_human
HgeneAK4C0086743Osteoarthrosis Deformans1CTD_human
TgeneC0023893Liver Cirrhosis, Experimental1CTD_human