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Center for Computational Systems Medicine
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Fusion Gene Summary

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Fusion Gene ORF analysis

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Fusion Genomic Features

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Fusion Protein Features

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Fusion Gene Sequence

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Fusion Gene PPI analysis

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Related Drugs

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Related Diseases

Fusion gene:DNAJB5-MYH9 (FusionGDB2 ID:HG25822TG4627)

Fusion Gene Summary for DNAJB5-MYH9

check button Fusion gene summary
Fusion gene informationFusion gene name: DNAJB5-MYH9
Fusion gene ID: hg25822tg4627
HgeneTgene
Gene symbol

DNAJB5

MYH9

Gene ID

25822

4627

Gene nameDnaJ heat shock protein family (Hsp40) member B5myosin heavy chain 9
SynonymsHsc40BDPLT6|DFNA17|EPSTS|FTNS|MATINS|MHA|NMHC-II-A|NMMHC-IIA|NMMHCA
Cytomap('DNAJB5')('MYH9')

9p13.3

22q12.3

Type of geneprotein-codingprotein-coding
DescriptiondnaJ homolog subfamily B member 5DnaJ (Hsp40) homolog, subfamily B, member 5heat shock cognate 40heat shock protein Hsp40-2heat shock protein Hsp40-3heat shock protein cognate 40myosin-9cellular myosin heavy chain, type Amyosin, heavy chain 9, non-musclenon-muscle myosin heavy chain 9non-muscle myosin heavy chain Anon-muscle myosin heavy chain IIanon-muscle myosin heavy polypeptide 9nonmuscle myosin heavy chain II-A
Modification date2020031320200315
UniProtAcc.

P35579

Ensembl transtripts involved in fusion geneENST00000453597, ENST00000312316, 
ENST00000335998, ENST00000454002, 
ENST00000541010, ENST00000545841, 
Fusion gene scores* DoF score1 X 1 X 1=144 X 46 X 15=30360
# samples 156
** MAII scorelog2(1/1*10)=3.32192809488736log2(56/30360*10)=-5.76060115335786
possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs).
DoF>8 and MAII<0
Context

PubMed: DNAJB5 [Title/Abstract] AND MYH9 [Title/Abstract] AND fusion [Title/Abstract]

Most frequent breakpointDNAJB5(34989828)-MYH9(36718566), # samples:1
Anticipated loss of major functional domain due to fusion event.DNAJB5-MYH9 seems lost the major protein functional domain in Tgene partner, which is a CGC due to the frame-shifted ORF.
DNAJB5-MYH9 seems lost the major protein functional domain in Tgene partner, which is a tumor suppressor due to the frame-shifted ORF.
* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

check button Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez
PartnerGeneGO IDGO termPubMed ID
TgeneMYH9

GO:0001525

angiogenesis

16403913

TgeneMYH9

GO:0001778

plasma membrane repair

27325790

TgeneMYH9

GO:0006509

membrane protein ectodomain proteolysis

16186248

TgeneMYH9

GO:0030048

actin filament-based movement

12237319|15845534

TgeneMYH9

GO:0031032

actomyosin structure organization

24072716



check button Fusion gene information
* All genome coordinats were lifted-over on hg19.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.
SourceDiseaseSampleHgeneHchrHbpHstrandTgeneTchrTbpTstrand
ChimerDB4STADTCGA-BR-A44T-01ADNAJB5chr9

34989828

+MYH9chr22

36718566

-


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Fusion Gene ORF analysis for DNAJB5-MYH9

check button Open reading frame (ORF) analsis of fusion genes based on Ensembl gene isoform structure.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.
ORFHenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrand
5CDS-intronENST00000453597ENST00000401701DNAJB5chr9

34989828

+MYH9chr22

36718566

-
5CDS-intronENST00000453597ENST00000475726DNAJB5chr9

34989828

+MYH9chr22

36718566

-
5UTR-3CDSENST00000312316ENST00000216181DNAJB5chr9

34989828

+MYH9chr22

36718566

-
5UTR-3CDSENST00000335998ENST00000216181DNAJB5chr9

34989828

+MYH9chr22

36718566

-
5UTR-intronENST00000312316ENST00000401701DNAJB5chr9

34989828

+MYH9chr22

36718566

-
5UTR-intronENST00000312316ENST00000475726DNAJB5chr9

34989828

+MYH9chr22

36718566

-
5UTR-intronENST00000335998ENST00000401701DNAJB5chr9

34989828

+MYH9chr22

36718566

-
5UTR-intronENST00000335998ENST00000475726DNAJB5chr9

34989828

+MYH9chr22

36718566

-
Frame-shiftENST00000453597ENST00000216181DNAJB5chr9

34989828

+MYH9chr22

36718566

-
intron-3CDSENST00000454002ENST00000216181DNAJB5chr9

34989828

+MYH9chr22

36718566

-
intron-3CDSENST00000541010ENST00000216181DNAJB5chr9

34989828

+MYH9chr22

36718566

-
intron-3CDSENST00000545841ENST00000216181DNAJB5chr9

34989828

+MYH9chr22

36718566

-
intron-intronENST00000454002ENST00000401701DNAJB5chr9

34989828

+MYH9chr22

36718566

-
intron-intronENST00000454002ENST00000475726DNAJB5chr9

34989828

+MYH9chr22

36718566

-
intron-intronENST00000541010ENST00000401701DNAJB5chr9

34989828

+MYH9chr22

36718566

-
intron-intronENST00000541010ENST00000475726DNAJB5chr9

34989828

+MYH9chr22

36718566

-
intron-intronENST00000545841ENST00000401701DNAJB5chr9

34989828

+MYH9chr22

36718566

-
intron-intronENST00000545841ENST00000475726DNAJB5chr9

34989828

+MYH9chr22

36718566

-

check buttonORFfinder result based on the fusion transcript sequence of in-frame fusion genes.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandSeq length
(transcript)		BP loci
(transcript)		Predicted start
(transcript)		Predicted stop
(transcript)		Seq length
(amino acids)

check buttonDeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandNo-coding scoreCoding score

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Fusion Genomic Features for DNAJB5-MYH9


check buttonFusionAI prediction of the potential fusion gene breakpoint based on the pre-mature RNA sequence context (+/- 5kb of individual partner genes, total 20kb length sequence). FusionAI is a fusion gene breakpoint classifier based on convolutional neural network by comparing the fusion positive and negative sequence context of ~ 20K fusion gene data. From here, we can have the relative potentency of the 20K genomic sequence how individual sequnce will be likely used as the gene fusion breakpoints.
HgeneHchrHbpHstrandTgeneTchrTbpTstrand1-pp (fusion gene breakpoint)


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Fusion Protein Features for DNAJB5-MYH9


check button Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/:34989828/:36718566)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
4. Go down and press 'Search' tab twice.
4. Go down to have the hyperlink of the search result.
5. Click the hyperlink.
6. See the FGviewer result for your fusion gene.
FGviewer

check buttonMain function of each fusion partner protein. (from UniProt)
HgeneTgene
.MYH9

P35579

FUNCTION: Transcriptional activator which is required for calcium-dependent dendritic growth and branching in cortical neurons. Recruits CREB-binding protein (CREBBP) to nuclear bodies. Component of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating a calcium-dependent release of a repressor complex and a recruitment of an activator complex. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex. At the same time, there is increased recruitment of CREBBP to the promoter by a CREST-dependent mechanism, which leads to transcriptional activation. The CREST-BRG1 complex also binds to the NR2B promoter, and activity-dependent induction of NR2B expression involves a release of HDAC1 and recruitment of CREBBP (By similarity). {ECO:0000250}.FUNCTION: Cellular myosin that appears to play a role in cytokinesis, cell shape, and specialized functions such as secretion and capping. Promotes also cell motility together with S100A4 (PubMed:16707441). During cell spreading, plays an important role in cytoskeleton reorganization, focal contacts formation (in the margins but not the central part of spreading cells), and lamellipodial retraction; this function is mechanically antagonized by MYH10 (PubMed:20052411). {ECO:0000250|UniProtKB:Q8VDD5, ECO:0000269|PubMed:16707441, ECO:0000269|PubMed:20052411}.

check buttonRetention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at

download page


* Minus value of BPloci means that the break pointn is located before the CDS.
- In-frame and retained protein feature among the 13 regional features.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note

- In-frame and not-retained protein feature among the 13 regional features.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note


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Fusion Gene Sequence for DNAJB5-MYH9


check button For in-frame fusion transcripts, we provide the fusion transcript sequences and fusion amino acid sequences. To have fusion amino acid sequence, we ran ORFfinder and chose the longest ORF among the all predicted ones.

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Fusion Gene PPI Analysis for DNAJB5-MYH9


check button Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in

ChiPPI page.


check button Protein-protein interactors with each fusion partner protein in wild-type (BIOGRID-3.4.160)
HgeneHgene's interactorsTgeneTgene's interactors


check button - Retained PPIs in in-frame fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenStill interaction with


check button - Lost PPIs in in-frame fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenInteraction lost with


check button - Retained PPIs, but lost function due to frame-shift fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenInteraction lost with


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Related Drugs for DNAJB5-MYH9


check button Drugs targeting genes involved in this fusion gene.
(DrugBank Version 5.1.8 2021-05-08)
PartnerGeneUniProtAccDrugBank IDDrug nameDrug activityDrug typeDrug status

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Related Diseases for DNAJB5-MYH9


check button Diseases associated with fusion partners.
(DisGeNet 4.0)
PartnerGeneDisease IDDisease name# pubmedsSource
TgeneC0340978May-Hegglin anomaly25CLINGEN;GENOMICS_ENGLAND;UNIPROT
TgeneC1854520SEBASTIAN SYNDROME14CLINGEN;CTD_human;GENOMICS_ENGLAND;ORPHANET
TgeneC0398641Epstein syndrome (disorder)11CLINGEN
TgeneC0403445Fechtner syndrome (disorder)11CLINGEN
TgeneC0477317Other primary thrombocytopenia11CLINGEN
TgeneC1842035Giant Platelet Syndrome with Thrombocytopenia11CLINGEN
TgeneC1863659DEAFNESS, AUTOSOMAL DOMINANT 176CTD_human;GENOMICS_ENGLAND;UNIPROT
TgeneC0022661Kidney Failure, Chronic2CTD_human
TgeneC0006142Malignant neoplasm of breast1CTD_human;UNIPROT
TgeneC0017668Focal glomerulosclerosis1CTD_human
TgeneC0018784Sensorineural Hearing Loss (disorder)1GENOMICS_ENGLAND
TgeneC0018965Hematuria1GENOMICS_ENGLAND
TgeneC0020544Renal hypertension1CTD_human
TgeneC0027626Neoplasm Invasiveness1CTD_human
TgeneC0027706Hereditary nephritis1CTD_human
TgeneC0033687Proteinuria1GENOMICS_ENGLAND
TgeneC0035078Kidney Failure1GENOMICS_ENGLAND
TgeneC0086432Hyalinosis, Segmental Glomerular1CTD_human
TgeneC0086543Cataract1GENOMICS_ENGLAND
TgeneC0206692Carcinoma, Lobular1CTD_human
TgeneC0410005Nodular fasciitis1ORPHANET
TgeneC0678222Breast Carcinoma1CTD_human
TgeneC1257931Mammary Neoplasms, Human1CTD_human
TgeneC1458155Mammary Neoplasms1CTD_human
TgeneC1567741Alport Syndrome1CTD_human
TgeneC1567742Alport Syndrome, X-Linked1CTD_human
TgeneC1567743Alport Syndrome, Autosomal Dominant1CTD_human
TgeneC1567744Alport Syndrome, Autosomal Recessive1CTD_human
TgeneC1834478MACROTHROMBOCYTOPENIA AND PROGRESSIVE SENSORINEURAL DEAFNESS1CTD_human
TgeneC2931861Hemorrhagic hereditary nephritis1CTD_human
TgeneC4280711Leukocyte inclusion bodies1GENOMICS_ENGLAND
TgeneC4704874Mammary Carcinoma, Human1CTD_human