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Center for Computational Systems Medicine
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Fusion Gene Summary

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Fusion Gene ORF analysis

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Fusion Genomic Features

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Fusion Protein Features

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Fusion Gene Sequence

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Fusion Gene PPI analysis

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Related Drugs

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Related Diseases

Fusion gene:HERC4-RAB4A (FusionGDB2 ID:HG26091TG5867)

Fusion Gene Summary for HERC4-RAB4A

check button Fusion gene summary
Fusion gene informationFusion gene name: HERC4-RAB4A
Fusion gene ID: hg26091tg5867
HgeneTgene
Gene symbol

HERC4

RAB4A

Gene ID

26091

5867

Gene nameHECT and RLD domain containing E3 ubiquitin protein ligase 4RAB4A, member RAS oncogene family
Synonyms-HRES-1|HRES-1/RAB4|HRES1|RAB4
Cytomap('HERC4')('RAB4A')

10q21.3

1q42.13

Type of geneprotein-codingprotein-coding
Descriptionprobable E3 ubiquitin-protein ligase HERC4HECT domain and RCC1-like domain-containing protein 4HECT-type E3 ubiquitin transferase HERC4hect domain and RLD 4ras-related protein Rab-4AHTLV-1 related endogenous sequence
Modification date2020032720200313
UniProtAcc..
Ensembl transtripts involved in fusion geneENST00000395187, ENST00000277817, 
ENST00000373700, ENST00000395198, 
ENST00000412272, ENST00000480158, 
ENST00000492996, 
Fusion gene scores* DoF score12 X 13 X 6=9365 X 4 X 4=80
# samples 155
** MAII scorelog2(15/936*10)=-2.64154602908752
possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs).
DoF>8 and MAII<0
log2(5/80*10)=-0.678071905112638
possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs).
DoF>8 and MAII<0
Context

PubMed: HERC4 [Title/Abstract] AND RAB4A [Title/Abstract] AND fusion [Title/Abstract]

Most frequent breakpointHERC4(69773783)-RAB4A(229431595), # samples:1
Anticipated loss of major functional domain due to fusion event.HERC4-RAB4A seems lost the major protein functional domain in Hgene partner, which is a essential gene due to the frame-shifted ORF.
HERC4-RAB4A seems lost the major protein functional domain in Tgene partner, which is a essential gene due to the frame-shifted ORF.
* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

check button Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez
PartnerGeneGO IDGO termPubMed ID


check button Fusion gene information
* All genome coordinats were lifted-over on hg19.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.
SourceDiseaseSampleHgeneHchrHbpHstrandTgeneTchrTbpTstrand
ChimerDB4STADTCGA-IN-7808-01AHERC4chr10

69773783

-RAB4Achr1

229431595

+


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Fusion Gene ORF analysis for HERC4-RAB4A

check button Open reading frame (ORF) analsis of fusion genes based on Ensembl gene isoform structure.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.
ORFHenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrand
3UTR-3CDSENST00000395187ENST00000366690HERC4chr10

69773783

-RAB4Achr1

229431595

+
3UTR-3UTRENST00000395187ENST00000473894HERC4chr10

69773783

-RAB4Achr1

229431595

+
5CDS-3UTRENST00000277817ENST00000473894HERC4chr10

69773783

-RAB4Achr1

229431595

+
5CDS-3UTRENST00000373700ENST00000473894HERC4chr10

69773783

-RAB4Achr1

229431595

+
5CDS-3UTRENST00000395198ENST00000473894HERC4chr10

69773783

-RAB4Achr1

229431595

+
5CDS-3UTRENST00000412272ENST00000473894HERC4chr10

69773783

-RAB4Achr1

229431595

+
Frame-shiftENST00000277817ENST00000366690HERC4chr10

69773783

-RAB4Achr1

229431595

+
Frame-shiftENST00000373700ENST00000366690HERC4chr10

69773783

-RAB4Achr1

229431595

+
Frame-shiftENST00000395198ENST00000366690HERC4chr10

69773783

-RAB4Achr1

229431595

+
Frame-shiftENST00000412272ENST00000366690HERC4chr10

69773783

-RAB4Achr1

229431595

+
intron-3CDSENST00000480158ENST00000366690HERC4chr10

69773783

-RAB4Achr1

229431595

+
intron-3CDSENST00000492996ENST00000366690HERC4chr10

69773783

-RAB4Achr1

229431595

+
intron-3UTRENST00000480158ENST00000473894HERC4chr10

69773783

-RAB4Achr1

229431595

+
intron-3UTRENST00000492996ENST00000473894HERC4chr10

69773783

-RAB4Achr1

229431595

+

check buttonORFfinder result based on the fusion transcript sequence of in-frame fusion genes.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandSeq length
(transcript)
BP loci
(transcript)
Predicted start
(transcript)
Predicted stop
(transcript)
Seq length
(amino acids)

check buttonDeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandNo-coding scoreCoding score

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Fusion Genomic Features for HERC4-RAB4A


check buttonFusionAI prediction of the potential fusion gene breakpoint based on the pre-mature RNA sequence context (+/- 5kb of individual partner genes, total 20kb length sequence). FusionAI is a fusion gene breakpoint classifier based on convolutional neural network by comparing the fusion positive and negative sequence context of ~ 20K fusion gene data. From here, we can have the relative potentency of the 20K genomic sequence how individual sequnce will be likely used as the gene fusion breakpoints.
HgeneHchrHbpHstrandTgeneTchrTbpTstrand1-pp (fusion gene breakpoint)
HERC4chr1069773782-RAB4Achr1229431594+0.039474840.96052516
HERC4chr1069773782-RAB4Achr1229431594+0.039474840.96052516


check buttonDistribution of 44 human genomic features loci across 20kb length fusion breakpoint regions that are ovelapped with the top 1% feature importance score regions. More details are in help page.
genomic feature of top 1%

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Fusion Protein Features for HERC4-RAB4A


check button Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/:69773783/:229431595)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
4. Go down and press 'Search' tab twice.
4. Go down to have the hyperlink of the search result.
5. Click the hyperlink.
6. See the FGviewer result for your fusion gene.
FGviewer

check buttonMain function of each fusion partner protein. (from UniProt)
HgeneTgene
..
FUNCTION: Transcriptional activator which is required for calcium-dependent dendritic growth and branching in cortical neurons. Recruits CREB-binding protein (CREBBP) to nuclear bodies. Component of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating a calcium-dependent release of a repressor complex and a recruitment of an activator complex. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex. At the same time, there is increased recruitment of CREBBP to the promoter by a CREST-dependent mechanism, which leads to transcriptional activation. The CREST-BRG1 complex also binds to the NR2B promoter, and activity-dependent induction of NR2B expression involves a release of HDAC1 and recruitment of CREBBP (By similarity). {ECO:0000250}.FUNCTION: Transcriptional activator which is required for calcium-dependent dendritic growth and branching in cortical neurons. Recruits CREB-binding protein (CREBBP) to nuclear bodies. Component of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating a calcium-dependent release of a repressor complex and a recruitment of an activator complex. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex. At the same time, there is increased recruitment of CREBBP to the promoter by a CREST-dependent mechanism, which leads to transcriptional activation. The CREST-BRG1 complex also binds to the NR2B promoter, and activity-dependent induction of NR2B expression involves a release of HDAC1 and recruitment of CREBBP (By similarity). {ECO:0000250}.

check buttonRetention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at

download page


* Minus value of BPloci means that the break pointn is located before the CDS.
- In-frame and retained protein feature among the 13 regional features.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note

- In-frame and not-retained protein feature among the 13 regional features.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note


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Fusion Gene Sequence for HERC4-RAB4A


check button For in-frame fusion transcripts, we provide the fusion transcript sequences and fusion amino acid sequences. To have fusion amino acid sequence, we ran ORFfinder and chose the longest ORF among the all predicted ones.

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Fusion Gene PPI Analysis for HERC4-RAB4A


check button Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in

ChiPPI page.


check button Protein-protein interactors with each fusion partner protein in wild-type (BIOGRID-3.4.160)
HgeneHgene's interactorsTgeneTgene's interactors


check button - Retained PPIs in in-frame fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenStill interaction with


check button - Lost PPIs in in-frame fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenInteraction lost with


check button - Retained PPIs, but lost function due to frame-shift fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenInteraction lost with


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Related Drugs for HERC4-RAB4A


check button Drugs targeting genes involved in this fusion gene.
(DrugBank Version 5.1.8 2021-05-08)
PartnerGeneUniProtAccDrugBank IDDrug nameDrug activityDrug typeDrug status

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Related Diseases for HERC4-RAB4A


check button Diseases associated with fusion partners.
(DisGeNet 4.0)
PartnerGeneDisease IDDisease name# pubmedsSource