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Center for Computational Systems Medicine
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Fusion Gene Summary

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Fusion Gene ORF analysis

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Fusion Genomic Features

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Fusion Protein Features

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Fusion Gene Sequence

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Fusion Gene PPI analysis

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Related Drugs

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Related Diseases

Fusion gene:RASSF3-DAZAP2 (FusionGDB2 ID:HG283349TG9802)

Fusion Gene Summary for RASSF3-DAZAP2

check button Fusion gene summary
Fusion gene informationFusion gene name: RASSF3-DAZAP2
Fusion gene ID: hg283349tg9802
HgeneTgene
Gene symbol

RASSF3

DAZAP2

Gene ID

283349

9802

Gene nameRas association domain family member 3DAZ associated protein 2
SynonymsRASSF5PRTB
Cytomap('RASSF3')('DAZAP2')

12q14.2

12q13.13

Type of geneprotein-codingprotein-coding
Descriptionras association domain-containing protein 3Ras association (RalGDS/AF-6) domain family 3Ras association (RalGDS/AF-6) domain family member 3DAZ-associated protein 2deleted in azoospermia associated protein 2proline-rich transcript in brainproline-rich transcript, brain-expressed protein
Modification date2020032720200313
UniProtAcc..
Ensembl transtripts involved in fusion geneENST00000540088, ENST00000336061, 
ENST00000542104, 
Fusion gene scores* DoF score18 X 7 X 12=15127 X 10 X 4=280
# samples 1810
** MAII scorelog2(18/1512*10)=-3.0703893278914
possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs).
DoF>8 and MAII<0
log2(10/280*10)=-1.48542682717024
possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs).
DoF>8 and MAII<0
Context

PubMed: RASSF3 [Title/Abstract] AND DAZAP2 [Title/Abstract] AND fusion [Title/Abstract]

Most frequent breakpointRASSF3(65004523)-DAZAP2(51634127), # samples:2
Anticipated loss of major functional domain due to fusion event.RASSF3-DAZAP2 seems lost the major protein functional domain in Hgene partner, which is a tumor suppressor due to the frame-shifted ORF.
RASSF3-DAZAP2 seems lost the major protein functional domain in Tgene partner, which is a essential gene due to the frame-shifted ORF.
* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

check button Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez
PartnerGeneGO IDGO termPubMed ID


check button Fusion gene information
* All genome coordinats were lifted-over on hg19.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.
SourceDiseaseSampleHgeneHchrHbpHstrandTgeneTchrTbpTstrand
ChimerDB4ACCTCGA-OR-A5LO-01ARASSF3chr12

65004523

+DAZAP2chr12

51634127

+
ChimerDB4ACCTCGA-OR-A5LORASSF3chr12

65004523

+DAZAP2chr12

51634126

+


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Fusion Gene ORF analysis for RASSF3-DAZAP2

check button Open reading frame (ORF) analsis of fusion genes based on Ensembl gene isoform structure.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.
ORFHenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrand
3UTR-3CDSENST00000540088ENST00000412716RASSF3chr12

65004523

+DAZAP2chr12

51634127

+
3UTR-3CDSENST00000540088ENST00000412716RASSF3chr12

65004523

+DAZAP2chr12

51634126

+
3UTR-3CDSENST00000540088ENST00000425012RASSF3chr12

65004523

+DAZAP2chr12

51634127

+
3UTR-3CDSENST00000540088ENST00000425012RASSF3chr12

65004523

+DAZAP2chr12

51634126

+
3UTR-3CDSENST00000540088ENST00000439799RASSF3chr12

65004523

+DAZAP2chr12

51634127

+
3UTR-3CDSENST00000540088ENST00000439799RASSF3chr12

65004523

+DAZAP2chr12

51634126

+
3UTR-3CDSENST00000540088ENST00000449723RASSF3chr12

65004523

+DAZAP2chr12

51634127

+
3UTR-3CDSENST00000540088ENST00000449723RASSF3chr12

65004523

+DAZAP2chr12

51634126

+
3UTR-3CDSENST00000540088ENST00000549555RASSF3chr12

65004523

+DAZAP2chr12

51634127

+
3UTR-3CDSENST00000540088ENST00000549555RASSF3chr12

65004523

+DAZAP2chr12

51634126

+
3UTR-3CDSENST00000540088ENST00000549732RASSF3chr12

65004523

+DAZAP2chr12

51634127

+
3UTR-3CDSENST00000540088ENST00000549732RASSF3chr12

65004523

+DAZAP2chr12

51634126

+
3UTR-3CDSENST00000540088ENST00000604900RASSF3chr12

65004523

+DAZAP2chr12

51634127

+
3UTR-3CDSENST00000540088ENST00000604900RASSF3chr12

65004523

+DAZAP2chr12

51634126

+
3UTR-3UTRENST00000540088ENST00000551534RASSF3chr12

65004523

+DAZAP2chr12

51634127

+
3UTR-3UTRENST00000540088ENST00000551534RASSF3chr12

65004523

+DAZAP2chr12

51634126

+
3UTR-5UTRENST00000540088ENST00000551313RASSF3chr12

65004523

+DAZAP2chr12

51634127

+
3UTR-5UTRENST00000540088ENST00000551313RASSF3chr12

65004523

+DAZAP2chr12

51634126

+
5CDS-3UTRENST00000336061ENST00000551534RASSF3chr12

65004523

+DAZAP2chr12

51634127

+
5CDS-3UTRENST00000336061ENST00000551534RASSF3chr12

65004523

+DAZAP2chr12

51634126

+
5CDS-3UTRENST00000542104ENST00000551534RASSF3chr12

65004523

+DAZAP2chr12

51634127

+
5CDS-3UTRENST00000542104ENST00000551534RASSF3chr12

65004523

+DAZAP2chr12

51634126

+
5CDS-5UTRENST00000336061ENST00000551313RASSF3chr12

65004523

+DAZAP2chr12

51634127

+
5CDS-5UTRENST00000336061ENST00000551313RASSF3chr12

65004523

+DAZAP2chr12

51634126

+
5CDS-5UTRENST00000542104ENST00000551313RASSF3chr12

65004523

+DAZAP2chr12

51634127

+
5CDS-5UTRENST00000542104ENST00000551313RASSF3chr12

65004523

+DAZAP2chr12

51634126

+
Frame-shiftENST00000336061ENST00000412716RASSF3chr12

65004523

+DAZAP2chr12

51634127

+
Frame-shiftENST00000336061ENST00000412716RASSF3chr12

65004523

+DAZAP2chr12

51634126

+
Frame-shiftENST00000336061ENST00000425012RASSF3chr12

65004523

+DAZAP2chr12

51634127

+
Frame-shiftENST00000336061ENST00000425012RASSF3chr12

65004523

+DAZAP2chr12

51634126

+
Frame-shiftENST00000336061ENST00000439799RASSF3chr12

65004523

+DAZAP2chr12

51634127

+
Frame-shiftENST00000336061ENST00000439799RASSF3chr12

65004523

+DAZAP2chr12

51634126

+
Frame-shiftENST00000336061ENST00000449723RASSF3chr12

65004523

+DAZAP2chr12

51634127

+
Frame-shiftENST00000336061ENST00000449723RASSF3chr12

65004523

+DAZAP2chr12

51634126

+
Frame-shiftENST00000336061ENST00000549555RASSF3chr12

65004523

+DAZAP2chr12

51634127

+
Frame-shiftENST00000336061ENST00000549555RASSF3chr12

65004523

+DAZAP2chr12

51634126

+
Frame-shiftENST00000336061ENST00000549732RASSF3chr12

65004523

+DAZAP2chr12

51634127

+
Frame-shiftENST00000336061ENST00000549732RASSF3chr12

65004523

+DAZAP2chr12

51634126

+
Frame-shiftENST00000336061ENST00000604900RASSF3chr12

65004523

+DAZAP2chr12

51634127

+
Frame-shiftENST00000336061ENST00000604900RASSF3chr12

65004523

+DAZAP2chr12

51634126

+
Frame-shiftENST00000542104ENST00000412716RASSF3chr12

65004523

+DAZAP2chr12

51634127

+
Frame-shiftENST00000542104ENST00000412716RASSF3chr12

65004523

+DAZAP2chr12

51634126

+
Frame-shiftENST00000542104ENST00000425012RASSF3chr12

65004523

+DAZAP2chr12

51634127

+
Frame-shiftENST00000542104ENST00000425012RASSF3chr12

65004523

+DAZAP2chr12

51634126

+
Frame-shiftENST00000542104ENST00000439799RASSF3chr12

65004523

+DAZAP2chr12

51634127

+
Frame-shiftENST00000542104ENST00000439799RASSF3chr12

65004523

+DAZAP2chr12

51634126

+
Frame-shiftENST00000542104ENST00000449723RASSF3chr12

65004523

+DAZAP2chr12

51634127

+
Frame-shiftENST00000542104ENST00000449723RASSF3chr12

65004523

+DAZAP2chr12

51634126

+
Frame-shiftENST00000542104ENST00000549555RASSF3chr12

65004523

+DAZAP2chr12

51634127

+
Frame-shiftENST00000542104ENST00000549555RASSF3chr12

65004523

+DAZAP2chr12

51634126

+
Frame-shiftENST00000542104ENST00000549732RASSF3chr12

65004523

+DAZAP2chr12

51634127

+
Frame-shiftENST00000542104ENST00000549732RASSF3chr12

65004523

+DAZAP2chr12

51634126

+
Frame-shiftENST00000542104ENST00000604900RASSF3chr12

65004523

+DAZAP2chr12

51634127

+
Frame-shiftENST00000542104ENST00000604900RASSF3chr12

65004523

+DAZAP2chr12

51634126

+

check buttonORFfinder result based on the fusion transcript sequence of in-frame fusion genes.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandSeq length
(transcript)
BP loci
(transcript)
Predicted start
(transcript)
Predicted stop
(transcript)
Seq length
(amino acids)

check buttonDeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandNo-coding scoreCoding score

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Fusion Genomic Features for RASSF3-DAZAP2


check buttonFusionAI prediction of the potential fusion gene breakpoint based on the pre-mature RNA sequence context (+/- 5kb of individual partner genes, total 20kb length sequence). FusionAI is a fusion gene breakpoint classifier based on convolutional neural network by comparing the fusion positive and negative sequence context of ~ 20K fusion gene data. From here, we can have the relative potentency of the 20K genomic sequence how individual sequnce will be likely used as the gene fusion breakpoints.
HgeneHchrHbpHstrandTgeneTchrTbpTstrand1-pp (fusion gene breakpoint)
RASSF3chr1265004523+DAZAP2chr1251634126+3.87E-111
RASSF3chr1265004523+DAZAP2chr1251634126+3.87E-111
RASSF3chr1265004523+DAZAP2chr1251634126+3.87E-111
RASSF3chr1265004523+DAZAP2chr1251634126+3.87E-111


check buttonDistribution of 44 human genomic features loci across 20kb length fusion breakpoint regions that are ovelapped with the top 1% feature importance score regions. More details are in help page.
genomic feature of top 1%

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Fusion Protein Features for RASSF3-DAZAP2


check button Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/:65004523/:51634127)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
4. Go down and press 'Search' tab twice.
4. Go down to have the hyperlink of the search result.
5. Click the hyperlink.
6. See the FGviewer result for your fusion gene.
FGviewer

check buttonMain function of each fusion partner protein. (from UniProt)
HgeneTgene
..
FUNCTION: Transcriptional activator which is required for calcium-dependent dendritic growth and branching in cortical neurons. Recruits CREB-binding protein (CREBBP) to nuclear bodies. Component of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating a calcium-dependent release of a repressor complex and a recruitment of an activator complex. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex. At the same time, there is increased recruitment of CREBBP to the promoter by a CREST-dependent mechanism, which leads to transcriptional activation. The CREST-BRG1 complex also binds to the NR2B promoter, and activity-dependent induction of NR2B expression involves a release of HDAC1 and recruitment of CREBBP (By similarity). {ECO:0000250}.FUNCTION: Transcriptional activator which is required for calcium-dependent dendritic growth and branching in cortical neurons. Recruits CREB-binding protein (CREBBP) to nuclear bodies. Component of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating a calcium-dependent release of a repressor complex and a recruitment of an activator complex. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex. At the same time, there is increased recruitment of CREBBP to the promoter by a CREST-dependent mechanism, which leads to transcriptional activation. The CREST-BRG1 complex also binds to the NR2B promoter, and activity-dependent induction of NR2B expression involves a release of HDAC1 and recruitment of CREBBP (By similarity). {ECO:0000250}.

check buttonRetention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at

download page


* Minus value of BPloci means that the break pointn is located before the CDS.
- In-frame and retained protein feature among the 13 regional features.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note

- In-frame and not-retained protein feature among the 13 regional features.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note


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Fusion Gene Sequence for RASSF3-DAZAP2


check button For in-frame fusion transcripts, we provide the fusion transcript sequences and fusion amino acid sequences. To have fusion amino acid sequence, we ran ORFfinder and chose the longest ORF among the all predicted ones.

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Fusion Gene PPI Analysis for RASSF3-DAZAP2


check button Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in

ChiPPI page.


check button Protein-protein interactors with each fusion partner protein in wild-type (BIOGRID-3.4.160)
HgeneHgene's interactorsTgeneTgene's interactors


check button - Retained PPIs in in-frame fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenStill interaction with


check button - Lost PPIs in in-frame fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenInteraction lost with


check button - Retained PPIs, but lost function due to frame-shift fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenInteraction lost with


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Related Drugs for RASSF3-DAZAP2


check button Drugs targeting genes involved in this fusion gene.
(DrugBank Version 5.1.8 2021-05-08)
PartnerGeneUniProtAccDrugBank IDDrug nameDrug activityDrug typeDrug status

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Related Diseases for RASSF3-DAZAP2


check button Diseases associated with fusion partners.
(DisGeNet 4.0)
PartnerGeneDisease IDDisease name# pubmedsSource
HgeneRASSF3C2931456Prostate cancer, familial1CTD_human
HgeneRASSF3C4722327PROSTATE CANCER, HEREDITARY, 11CTD_human