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Center for Computational Systems Medicine
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Fusion Gene Summary

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Fusion Gene ORF analysis

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Fusion Genomic Features

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Fusion Protein Features

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Fusion Gene Sequence

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Fusion Gene PPI analysis

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Related Drugs

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Related Diseases

Fusion gene:GSTA4-NADSYN1 (FusionGDB2 ID:HG2941TG55191)

Fusion Gene Summary for GSTA4-NADSYN1

check button Fusion gene summary
Fusion gene informationFusion gene name: GSTA4-NADSYN1
Fusion gene ID: hg2941tg55191
HgeneTgene
Gene symbol

GSTA4

NADSYN1

Gene ID

2941

55191

Gene nameglutathione S-transferase alpha 4NAD synthetase 1
SynonymsGSTA4-4|GTA4-
Cytomap('GSTA4')('NADSYN1')

6p12.2

11q13.4

Type of geneprotein-codingprotein-coding
Descriptionglutathione S-transferase A4GST class-alpha member 4S-(hydroxyalkyl)glutathione lyase A4glutathione S-alkyltransferase A4glutathione S-aralkyltransferase A4glutathione S-aryltransferase A4glutathione S-transferase A4-4glutathione transferase A4-4glutamine-dependent NAD(+) synthetaseNAD(+) synthaseNAD(+) synthetaseglutamine-dependent NAD synthetase
Modification date2020032020200313
UniProtAcc

O15217

Q6IA69

Ensembl transtripts involved in fusion geneENST00000370959, ENST00000370960, 
ENST00000541324, ENST00000486559, 
Fusion gene scores* DoF score3 X 3 X 3=2715 X 16 X 7=1680
# samples 318
** MAII scorelog2(3/27*10)=0.15200309344505
effective Gene in Pan-Cancer Fusion Genes (eGinPCFGs).
DoF>8 and MAII>0
log2(18/1680*10)=-3.22239242133645
possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs).
DoF>8 and MAII<0
Context

PubMed: GSTA4 [Title/Abstract] AND NADSYN1 [Title/Abstract] AND fusion [Title/Abstract]

Most frequent breakpointGSTA4(52847372)-NADSYN1(71208529), # samples:3
Anticipated loss of major functional domain due to fusion event.GSTA4-NADSYN1 seems lost the major protein functional domain in Hgene partner, which is a cell metabolism gene due to the frame-shifted ORF.
GSTA4-NADSYN1 seems lost the major protein functional domain in Tgene partner, which is a cell metabolism gene due to the frame-shifted ORF.
* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

check button Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez
PartnerGeneGO IDGO termPubMed ID
HgeneGSTA4

GO:0006749

glutathione metabolic process

10329152

HgeneGSTA4

GO:0006805

xenobiotic metabolic process

10329152



check button Fusion gene information
* All genome coordinats were lifted-over on hg19.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.
SourceDiseaseSampleHgeneHchrHbpHstrandTgeneTchrTbpTstrand
ChimerDB4BRCATCGA-A2-A0T4-01AGSTA4chr6

52847372

-NADSYN1chr11

71208529

+


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Fusion Gene ORF analysis for GSTA4-NADSYN1

check button Open reading frame (ORF) analsis of fusion genes based on Ensembl gene isoform structure.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.
ORFHenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrand
5CDS-intronENST00000370959ENST00000526039GSTA4chr6

52847372

-NADSYN1chr11

71208529

+
5CDS-intronENST00000370960ENST00000526039GSTA4chr6

52847372

-NADSYN1chr11

71208529

+
5CDS-intronENST00000541324ENST00000526039GSTA4chr6

52847372

-NADSYN1chr11

71208529

+
5UTR-3CDSENST00000486559ENST00000319023GSTA4chr6

52847372

-NADSYN1chr11

71208529

+
5UTR-3CDSENST00000486559ENST00000530055GSTA4chr6

52847372

-NADSYN1chr11

71208529

+
5UTR-3CDSENST00000486559ENST00000539574GSTA4chr6

52847372

-NADSYN1chr11

71208529

+
5UTR-intronENST00000486559ENST00000526039GSTA4chr6

52847372

-NADSYN1chr11

71208529

+
Frame-shiftENST00000370959ENST00000319023GSTA4chr6

52847372

-NADSYN1chr11

71208529

+
Frame-shiftENST00000370959ENST00000530055GSTA4chr6

52847372

-NADSYN1chr11

71208529

+
Frame-shiftENST00000370959ENST00000539574GSTA4chr6

52847372

-NADSYN1chr11

71208529

+
Frame-shiftENST00000370960ENST00000319023GSTA4chr6

52847372

-NADSYN1chr11

71208529

+
Frame-shiftENST00000370960ENST00000530055GSTA4chr6

52847372

-NADSYN1chr11

71208529

+
Frame-shiftENST00000370960ENST00000539574GSTA4chr6

52847372

-NADSYN1chr11

71208529

+
Frame-shiftENST00000541324ENST00000319023GSTA4chr6

52847372

-NADSYN1chr11

71208529

+
Frame-shiftENST00000541324ENST00000530055GSTA4chr6

52847372

-NADSYN1chr11

71208529

+
Frame-shiftENST00000541324ENST00000539574GSTA4chr6

52847372

-NADSYN1chr11

71208529

+

check buttonORFfinder result based on the fusion transcript sequence of in-frame fusion genes.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandSeq length
(transcript)
BP loci
(transcript)
Predicted start
(transcript)
Predicted stop
(transcript)
Seq length
(amino acids)

check buttonDeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandNo-coding scoreCoding score

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Fusion Genomic Features for GSTA4-NADSYN1


check buttonFusionAI prediction of the potential fusion gene breakpoint based on the pre-mature RNA sequence context (+/- 5kb of individual partner genes, total 20kb length sequence). FusionAI is a fusion gene breakpoint classifier based on convolutional neural network by comparing the fusion positive and negative sequence context of ~ 20K fusion gene data. From here, we can have the relative potentency of the 20K genomic sequence how individual sequnce will be likely used as the gene fusion breakpoints.
HgeneHchrHbpHstrandTgeneTchrTbpTstrand1-pp (fusion gene breakpoint)
GSTA4chr652847371-NADSYN1chr1171208528+0.096265660.9037344
GSTA4chr652847371-NADSYN1chr1171208528+0.096265660.9037344


check buttonDistribution of 44 human genomic features loci across 20kb length fusion breakpoint regions that are ovelapped with the top 1% feature importance score regions. More details are in help page.
genomic feature of top 1%

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Fusion Protein Features for GSTA4-NADSYN1


check button Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/:52847372/:71208529)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
4. Go down and press 'Search' tab twice.
4. Go down to have the hyperlink of the search result.
5. Click the hyperlink.
6. See the FGviewer result for your fusion gene.
FGviewer

check buttonMain function of each fusion partner protein. (from UniProt)
HgeneTgene
GSTA4

O15217

NADSYN1

Q6IA69

FUNCTION: Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. This isozyme has a high catalytic efficiency with 4-hydroxyalkenals such as 4-hydroxynonenal (4-HNE). {ECO:0000269|PubMed:10329152, ECO:0000269|PubMed:20085333}.FUNCTION: Catalyzes the final step of the nicotinamide adenine dinucleotide (NAD) de novo synthesis pathway, the ATP-dependent amidation of deamido-NAD using L-glutamine as a nitrogen source. {ECO:0000269|PubMed:12547821, ECO:0000269|PubMed:31883644}.

check buttonRetention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at

download page


* Minus value of BPloci means that the break pointn is located before the CDS.
- In-frame and retained protein feature among the 13 regional features.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note

- In-frame and not-retained protein feature among the 13 regional features.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note


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Fusion Gene Sequence for GSTA4-NADSYN1


check button For in-frame fusion transcripts, we provide the fusion transcript sequences and fusion amino acid sequences. To have fusion amino acid sequence, we ran ORFfinder and chose the longest ORF among the all predicted ones.

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Fusion Gene PPI Analysis for GSTA4-NADSYN1


check button Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in

ChiPPI page.


check button Protein-protein interactors with each fusion partner protein in wild-type (BIOGRID-3.4.160)
HgeneHgene's interactorsTgeneTgene's interactors


check button - Retained PPIs in in-frame fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenStill interaction with


check button - Lost PPIs in in-frame fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenInteraction lost with


check button - Retained PPIs, but lost function due to frame-shift fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenInteraction lost with


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Related Drugs for GSTA4-NADSYN1


check button Drugs targeting genes involved in this fusion gene.
(DrugBank Version 5.1.8 2021-05-08)
PartnerGeneUniProtAccDrugBank IDDrug nameDrug activityDrug typeDrug status
HgeneGSTA4O15217DB00143GlutathioneSmall moleculeApproved|Investigational|Nutraceutical
TgeneNADSYN1Q6IA69DB00142Glutamic acidProduct ofSmall moleculeApproved|Nutraceutical

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Related Diseases for GSTA4-NADSYN1


check button Diseases associated with fusion partners.
(DisGeNet 4.0)
PartnerGeneDisease IDDisease name# pubmedsSource
HgeneGSTA4C0003850Arteriosclerosis2CTD_human
HgeneGSTA4C0019193Hepatitis, Toxic1CTD_human
HgeneGSTA4C0030567Parkinson Disease1CTD_human
HgeneGSTA4C0032927Precancerous Conditions1CTD_human
HgeneGSTA4C0282313Condition, Preneoplastic1CTD_human
HgeneGSTA4C0860207Drug-Induced Liver Disease1CTD_human
HgeneGSTA4C1262760Hepatitis, Drug-Induced1CTD_human
HgeneGSTA4C3658290Drug-Induced Acute Liver Injury1CTD_human
HgeneGSTA4C4277682Chemical and Drug Induced Liver Injury1CTD_human
HgeneGSTA4C4279912Chemically-Induced Liver Toxicity1CTD_human
TgeneC0000772Multiple congenital anomalies1GENOMICS_ENGLAND
TgeneC0426706Absent kidney1GENOMICS_ENGLAND