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Center for Computational Systems Medicine
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Fusion Gene Summary

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Fusion Gene ORF analysis

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Fusion Genomic Features

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Fusion Protein Features

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Fusion Gene Sequence

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Fusion Gene PPI analysis

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Related Drugs

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Related Diseases

Fusion gene:ADAM22-ASB4 (FusionGDB2 ID:HG53616TG51666)

Fusion Gene Summary for ADAM22-ASB4

check button Fusion gene summary
Fusion gene informationFusion gene name: ADAM22-ASB4
Fusion gene ID: hg53616tg51666
HgeneTgene
Gene symbol

ADAM22

ASB4

Gene ID

53616

51666

Gene nameADAM metallopeptidase domain 22ankyrin repeat and SOCS box containing 4
SynonymsADAM 22|EIEE61|MDC2ASB-4
Cytomap('ADAM22')('ASB4')

7q21.12

7q21.3

Type of geneprotein-codingprotein-coding
Descriptiondisintegrin and metalloproteinase domain-containing protein 22a disintegrin and metalloproteinase domain 22metalloproteinase-disintegrin ADAM22-3metalloproteinase-like, disintegrin-like, and cysteine-rich protein 2ankyrin repeat and SOCS box protein 4
Modification date2020031320200313
UniProtAcc.

Q9Y574

Ensembl transtripts involved in fusion geneENST00000265727, ENST00000315984, 
ENST00000398201, ENST00000398204, 
ENST00000398209, ENST00000439864, 
ENST00000476330, 
Fusion gene scores* DoF score3 X 3 X 3=273 X 3 X 3=27
# samples 33
** MAII scorelog2(3/27*10)=0.15200309344505
effective Gene in Pan-Cancer Fusion Genes (eGinPCFGs).
DoF>8 and MAII>0
log2(3/27*10)=0.15200309344505
effective Gene in Pan-Cancer Fusion Genes (eGinPCFGs).
DoF>8 and MAII>0
Context

PubMed: ADAM22 [Title/Abstract] AND ASB4 [Title/Abstract] AND fusion [Title/Abstract]

Most frequent breakpointADAM22(87754974)-ASB4(95166883), # samples:2
Anticipated loss of major functional domain due to fusion event.ADAM22-ASB4 seems lost the major protein functional domain in Hgene partner, which is a IUPHAR drug target due to the frame-shifted ORF.
* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

check button Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez
PartnerGeneGO IDGO termPubMed ID


check button Fusion gene information
* All genome coordinats were lifted-over on hg19.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.
SourceDiseaseSampleHgeneHchrHbpHstrandTgeneTchrTbpTstrand
ChimerDB4KIRPTCGA-B9-4116-01AADAM22chr7

87754974

-ASB4chr7

95166883

+
ChimerDB4KIRPTCGA-B9-4116-01AADAM22chr7

87754974

+ASB4chr7

95166883

+


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Fusion Gene ORF analysis for ADAM22-ASB4

check button Open reading frame (ORF) analsis of fusion genes based on Ensembl gene isoform structure.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.
ORFHenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrand
5CDS-intronENST00000265727ENST00000257621ADAM22chr7

87754974

+ASB4chr7

95166883

+
5CDS-intronENST00000265727ENST00000428113ADAM22chr7

87754974

+ASB4chr7

95166883

+
5CDS-intronENST00000315984ENST00000257621ADAM22chr7

87754974

+ASB4chr7

95166883

+
5CDS-intronENST00000315984ENST00000428113ADAM22chr7

87754974

+ASB4chr7

95166883

+
5CDS-intronENST00000398201ENST00000257621ADAM22chr7

87754974

+ASB4chr7

95166883

+
5CDS-intronENST00000398201ENST00000428113ADAM22chr7

87754974

+ASB4chr7

95166883

+
5CDS-intronENST00000398204ENST00000257621ADAM22chr7

87754974

+ASB4chr7

95166883

+
5CDS-intronENST00000398204ENST00000428113ADAM22chr7

87754974

+ASB4chr7

95166883

+
5CDS-intronENST00000398209ENST00000257621ADAM22chr7

87754974

+ASB4chr7

95166883

+
5CDS-intronENST00000398209ENST00000428113ADAM22chr7

87754974

+ASB4chr7

95166883

+
5CDS-intronENST00000439864ENST00000257621ADAM22chr7

87754974

+ASB4chr7

95166883

+
5CDS-intronENST00000439864ENST00000428113ADAM22chr7

87754974

+ASB4chr7

95166883

+
Frame-shiftENST00000265727ENST00000325885ADAM22chr7

87754974

+ASB4chr7

95166883

+
Frame-shiftENST00000315984ENST00000325885ADAM22chr7

87754974

+ASB4chr7

95166883

+
Frame-shiftENST00000398201ENST00000325885ADAM22chr7

87754974

+ASB4chr7

95166883

+
Frame-shiftENST00000398204ENST00000325885ADAM22chr7

87754974

+ASB4chr7

95166883

+
Frame-shiftENST00000398209ENST00000325885ADAM22chr7

87754974

+ASB4chr7

95166883

+
Frame-shiftENST00000439864ENST00000325885ADAM22chr7

87754974

+ASB4chr7

95166883

+
intron-3CDSENST00000476330ENST00000325885ADAM22chr7

87754974

+ASB4chr7

95166883

+
intron-intronENST00000476330ENST00000257621ADAM22chr7

87754974

+ASB4chr7

95166883

+
intron-intronENST00000476330ENST00000428113ADAM22chr7

87754974

+ASB4chr7

95166883

+

check buttonORFfinder result based on the fusion transcript sequence of in-frame fusion genes.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandSeq length
(transcript)
BP loci
(transcript)
Predicted start
(transcript)
Predicted stop
(transcript)
Seq length
(amino acids)

check buttonDeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandNo-coding scoreCoding score

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Fusion Genomic Features for ADAM22-ASB4


check buttonFusionAI prediction of the potential fusion gene breakpoint based on the pre-mature RNA sequence context (+/- 5kb of individual partner genes, total 20kb length sequence). FusionAI is a fusion gene breakpoint classifier based on convolutional neural network by comparing the fusion positive and negative sequence context of ~ 20K fusion gene data. From here, we can have the relative potentency of the 20K genomic sequence how individual sequnce will be likely used as the gene fusion breakpoints.
HgeneHchrHbpHstrandTgeneTchrTbpTstrand1-pp (fusion gene breakpoint)
ADAM22chr787754974+ASB4chr795166882+0.001835980.998164
ADAM22chr787754974+ASB4chr795166882+0.001835980.998164


check buttonDistribution of 44 human genomic features loci across 20kb length fusion breakpoint regions that are ovelapped with the top 1% feature importance score regions. More details are in help page.
genomic feature of top 1%

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Fusion Protein Features for ADAM22-ASB4


check button Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/:87754974/:95166883)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
4. Go down and press 'Search' tab twice.
4. Go down to have the hyperlink of the search result.
5. Click the hyperlink.
6. See the FGviewer result for your fusion gene.
FGviewer

check buttonMain function of each fusion partner protein. (from UniProt)
HgeneTgene
.ASB4

Q9Y574

FUNCTION: Transcriptional activator which is required for calcium-dependent dendritic growth and branching in cortical neurons. Recruits CREB-binding protein (CREBBP) to nuclear bodies. Component of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating a calcium-dependent release of a repressor complex and a recruitment of an activator complex. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex. At the same time, there is increased recruitment of CREBBP to the promoter by a CREST-dependent mechanism, which leads to transcriptional activation. The CREST-BRG1 complex also binds to the NR2B promoter, and activity-dependent induction of NR2B expression involves a release of HDAC1 and recruitment of CREBBP (By similarity). {ECO:0000250}.FUNCTION: Probable substrate-recognition component of a SCF-like ECS (Elongin-Cullin-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Promotes differentiation and maturation of the vascular lineage by an oxygen-dependent mechanism (By similarity). {ECO:0000250}.

check buttonRetention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at

download page


* Minus value of BPloci means that the break pointn is located before the CDS.
- In-frame and retained protein feature among the 13 regional features.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note

- In-frame and not-retained protein feature among the 13 regional features.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note


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Fusion Gene Sequence for ADAM22-ASB4


check button For in-frame fusion transcripts, we provide the fusion transcript sequences and fusion amino acid sequences. To have fusion amino acid sequence, we ran ORFfinder and chose the longest ORF among the all predicted ones.

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Fusion Gene PPI Analysis for ADAM22-ASB4


check button Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in

ChiPPI page.


check button Protein-protein interactors with each fusion partner protein in wild-type (BIOGRID-3.4.160)
HgeneHgene's interactorsTgeneTgene's interactors


check button - Retained PPIs in in-frame fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenStill interaction with


check button - Lost PPIs in in-frame fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenInteraction lost with


check button - Retained PPIs, but lost function due to frame-shift fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenInteraction lost with


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Related Drugs for ADAM22-ASB4


check button Drugs targeting genes involved in this fusion gene.
(DrugBank Version 5.1.8 2021-05-08)
PartnerGeneUniProtAccDrugBank IDDrug nameDrug activityDrug typeDrug status

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Related Diseases for ADAM22-ASB4


check button Diseases associated with fusion partners.
(DisGeNet 4.0)
PartnerGeneDisease IDDisease name# pubmedsSource
HgeneADAM22C4693688EPILEPTIC ENCEPHALOPATHY, EARLY INFANTILE, 612GENOMICS_ENGLAND;UNIPROT