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Center for Computational Systems Medicine
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Fusion Gene Summary

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Fusion Gene ORF analysis

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Fusion Genomic Features

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Fusion Protein Features

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Fusion Gene Sequence

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Fusion Gene PPI analysis

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Related Drugs

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Related Diseases

Fusion gene:CCDC157-EWSR1 (FusionGDB2 ID:HG550631TG2130)

Fusion Gene Summary for CCDC157-EWSR1

check button Fusion gene summary
Fusion gene informationFusion gene name: CCDC157-EWSR1
Fusion gene ID: hg550631tg2130
HgeneTgene
Gene symbol

CCDC157

EWSR1

Gene ID

550631

2130

Gene namecoiled-coil domain containing 157EWS RNA binding protein 1
Synonyms-EWS|EWS-FLI1|bK984G1.4
Cytomap('CCDC157')('EWSR1')

22q12.2

22q12.2

Type of geneprotein-codingprotein-coding
Descriptioncoiled-coil domain-containing protein 157RNA-binding protein EWSEWS RNA-binding protein variant 6Ewing sarcoma breakpoint region 1Ewings sarcoma EWS-Fli1 (type 1) oncogene
Modification date2020031320200329
UniProtAcc.

Q01844

Ensembl transtripts involved in fusion geneENST00000338306, ENST00000399824, 
ENST00000405659, 
Fusion gene scores* DoF score3 X 3 X 2=1817 X 58 X 10=9860
# samples 448
** MAII scorelog2(4/18*10)=1.15200309344505
effective Gene in Pan-Cancer Fusion Genes (eGinPCFGs).
DoF>8 and MAII>0
log2(48/9860*10)=-4.36048133565676
possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs).
DoF>8 and MAII<0
Context

PubMed: CCDC157 [Title/Abstract] AND EWSR1 [Title/Abstract] AND fusion [Title/Abstract]

Most frequent breakpointCCDC157(30753121)-EWSR1(29688126), # samples:3
Anticipated loss of major functional domain due to fusion event.
* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

check button Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez
PartnerGeneGO IDGO termPubMed ID


check button Fusion gene information
* All genome coordinats were lifted-over on hg19.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.
SourceDiseaseSampleHgeneHchrHbpHstrandTgeneTchrTbpTstrand
ChimerDB4UCSTCGA-NA-A4QV-01ACCDC157chr22

30753121

-EWSR1chr22

29688126

+
ChimerDB4UCSTCGA-NA-A4QV-01ACCDC157chr22

30753121

+EWSR1chr22

29688126

+
ChimerDB4UCSTCGA-NA-A4QVCCDC157chr22

30753121

+EWSR1chr22

29687554

+
ChimerDB4UCSTCGA-NA-A4QVCCDC157chr22

30753121

+EWSR1chr22

29688126

+


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Fusion Gene ORF analysis for CCDC157-EWSR1

check button Open reading frame (ORF) analsis of fusion genes based on Ensembl gene isoform structure.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.
ORFHenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrand
5UTR-3CDSENST00000338306ENST00000332050CCDC157chr22

30753121

+EWSR1chr22

29688126

+
5UTR-3CDSENST00000338306ENST00000397938CCDC157chr22

30753121

+EWSR1chr22

29688126

+
5UTR-3CDSENST00000338306ENST00000406548CCDC157chr22

30753121

+EWSR1chr22

29688126

+
5UTR-3CDSENST00000399824ENST00000332050CCDC157chr22

30753121

+EWSR1chr22

29688126

+
5UTR-3CDSENST00000399824ENST00000397938CCDC157chr22

30753121

+EWSR1chr22

29688126

+
5UTR-3CDSENST00000399824ENST00000406548CCDC157chr22

30753121

+EWSR1chr22

29688126

+
5UTR-3CDSENST00000405659ENST00000332050CCDC157chr22

30753121

+EWSR1chr22

29688126

+
5UTR-3CDSENST00000405659ENST00000397938CCDC157chr22

30753121

+EWSR1chr22

29688126

+
5UTR-3CDSENST00000405659ENST00000406548CCDC157chr22

30753121

+EWSR1chr22

29688126

+
5UTR-intronENST00000338306ENST00000331029CCDC157chr22

30753121

+EWSR1chr22

29688126

+
5UTR-intronENST00000338306ENST00000331029CCDC157chr22

30753121

+EWSR1chr22

29687554

+
5UTR-intronENST00000338306ENST00000332035CCDC157chr22

30753121

+EWSR1chr22

29688126

+
5UTR-intronENST00000338306ENST00000332035CCDC157chr22

30753121

+EWSR1chr22

29687554

+
5UTR-intronENST00000338306ENST00000332050CCDC157chr22

30753121

+EWSR1chr22

29687554

+
5UTR-intronENST00000338306ENST00000333395CCDC157chr22

30753121

+EWSR1chr22

29688126

+
5UTR-intronENST00000338306ENST00000333395CCDC157chr22

30753121

+EWSR1chr22

29687554

+
5UTR-intronENST00000338306ENST00000397938CCDC157chr22

30753121

+EWSR1chr22

29687554

+
5UTR-intronENST00000338306ENST00000406548CCDC157chr22

30753121

+EWSR1chr22

29687554

+
5UTR-intronENST00000338306ENST00000414183CCDC157chr22

30753121

+EWSR1chr22

29688126

+
5UTR-intronENST00000338306ENST00000414183CCDC157chr22

30753121

+EWSR1chr22

29687554

+
5UTR-intronENST00000399824ENST00000331029CCDC157chr22

30753121

+EWSR1chr22

29688126

+
5UTR-intronENST00000399824ENST00000331029CCDC157chr22

30753121

+EWSR1chr22

29687554

+
5UTR-intronENST00000399824ENST00000332035CCDC157chr22

30753121

+EWSR1chr22

29688126

+
5UTR-intronENST00000399824ENST00000332035CCDC157chr22

30753121

+EWSR1chr22

29687554

+
5UTR-intronENST00000399824ENST00000332050CCDC157chr22

30753121

+EWSR1chr22

29687554

+
5UTR-intronENST00000399824ENST00000333395CCDC157chr22

30753121

+EWSR1chr22

29688126

+
5UTR-intronENST00000399824ENST00000333395CCDC157chr22

30753121

+EWSR1chr22

29687554

+
5UTR-intronENST00000399824ENST00000397938CCDC157chr22

30753121

+EWSR1chr22

29687554

+
5UTR-intronENST00000399824ENST00000406548CCDC157chr22

30753121

+EWSR1chr22

29687554

+
5UTR-intronENST00000399824ENST00000414183CCDC157chr22

30753121

+EWSR1chr22

29688126

+
5UTR-intronENST00000399824ENST00000414183CCDC157chr22

30753121

+EWSR1chr22

29687554

+
5UTR-intronENST00000405659ENST00000331029CCDC157chr22

30753121

+EWSR1chr22

29688126

+
5UTR-intronENST00000405659ENST00000331029CCDC157chr22

30753121

+EWSR1chr22

29687554

+
5UTR-intronENST00000405659ENST00000332035CCDC157chr22

30753121

+EWSR1chr22

29688126

+
5UTR-intronENST00000405659ENST00000332035CCDC157chr22

30753121

+EWSR1chr22

29687554

+
5UTR-intronENST00000405659ENST00000332050CCDC157chr22

30753121

+EWSR1chr22

29687554

+
5UTR-intronENST00000405659ENST00000333395CCDC157chr22

30753121

+EWSR1chr22

29688126

+
5UTR-intronENST00000405659ENST00000333395CCDC157chr22

30753121

+EWSR1chr22

29687554

+
5UTR-intronENST00000405659ENST00000397938CCDC157chr22

30753121

+EWSR1chr22

29687554

+
5UTR-intronENST00000405659ENST00000406548CCDC157chr22

30753121

+EWSR1chr22

29687554

+
5UTR-intronENST00000405659ENST00000414183CCDC157chr22

30753121

+EWSR1chr22

29688126

+
5UTR-intronENST00000405659ENST00000414183CCDC157chr22

30753121

+EWSR1chr22

29687554

+

check buttonORFfinder result based on the fusion transcript sequence of in-frame fusion genes.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandSeq length
(transcript)
BP loci
(transcript)
Predicted start
(transcript)
Predicted stop
(transcript)
Seq length
(amino acids)

check buttonDeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandNo-coding scoreCoding score

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Fusion Genomic Features for CCDC157-EWSR1


check buttonFusionAI prediction of the potential fusion gene breakpoint based on the pre-mature RNA sequence context (+/- 5kb of individual partner genes, total 20kb length sequence). FusionAI is a fusion gene breakpoint classifier based on convolutional neural network by comparing the fusion positive and negative sequence context of ~ 20K fusion gene data. From here, we can have the relative potentency of the 20K genomic sequence how individual sequnce will be likely used as the gene fusion breakpoints.
HgeneHchrHbpHstrandTgeneTchrTbpTstrand1-pp (fusion gene breakpoint)
CCDC157chr2230753121+EWSR1chr2229687553+0.121860680.87813926
CCDC157chr2230753121+EWSR1chr2229688125+0.000201950.9997981
CCDC157chr2230753121+EWSR1chr2229688125+0.000201950.9997981
CCDC157chr2230753121+EWSR1chr2229687553+0.121860680.87813926
CCDC157chr2230753121+EWSR1chr2229688125+0.000201950.9997981
CCDC157chr2230753121+EWSR1chr2229688125+0.000201950.9997981


check buttonDistribution of 44 human genomic features loci across 20kb length fusion breakpoint regions that are ovelapped with the top 1% feature importance score regions. More details are in help page.
genomic feature of top 1%

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Fusion Protein Features for CCDC157-EWSR1


check button Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/:30753121/:29688126)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
4. Go down and press 'Search' tab twice.
4. Go down to have the hyperlink of the search result.
5. Click the hyperlink.
6. See the FGviewer result for your fusion gene.
FGviewer

check buttonMain function of each fusion partner protein. (from UniProt)
HgeneTgene
.EWSR1

Q01844

FUNCTION: Transcriptional activator which is required for calcium-dependent dendritic growth and branching in cortical neurons. Recruits CREB-binding protein (CREBBP) to nuclear bodies. Component of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating a calcium-dependent release of a repressor complex and a recruitment of an activator complex. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex. At the same time, there is increased recruitment of CREBBP to the promoter by a CREST-dependent mechanism, which leads to transcriptional activation. The CREST-BRG1 complex also binds to the NR2B promoter, and activity-dependent induction of NR2B expression involves a release of HDAC1 and recruitment of CREBBP (By similarity). {ECO:0000250}.FUNCTION: Might normally function as a transcriptional repressor. EWS-fusion-proteins (EFPS) may play a role in the tumorigenic process. They may disturb gene expression by mimicking, or interfering with the normal function of CTD-POLII within the transcription initiation complex. They may also contribute to an aberrant activation of the fusion protein target genes.

check buttonRetention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at

download page


* Minus value of BPloci means that the break pointn is located before the CDS.
- In-frame and retained protein feature among the 13 regional features.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note

- In-frame and not-retained protein feature among the 13 regional features.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note


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Fusion Gene Sequence for CCDC157-EWSR1


check button For in-frame fusion transcripts, we provide the fusion transcript sequences and fusion amino acid sequences. To have fusion amino acid sequence, we ran ORFfinder and chose the longest ORF among the all predicted ones.

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Fusion Gene PPI Analysis for CCDC157-EWSR1


check button Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in

ChiPPI page.


check button Protein-protein interactors with each fusion partner protein in wild-type (BIOGRID-3.4.160)
HgeneHgene's interactorsTgeneTgene's interactors


check button - Retained PPIs in in-frame fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenStill interaction with


check button - Lost PPIs in in-frame fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenInteraction lost with


check button - Retained PPIs, but lost function due to frame-shift fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenInteraction lost with


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Related Drugs for CCDC157-EWSR1


check button Drugs targeting genes involved in this fusion gene.
(DrugBank Version 5.1.8 2021-05-08)
PartnerGeneUniProtAccDrugBank IDDrug nameDrug activityDrug typeDrug status

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Related Diseases for CCDC157-EWSR1


check button Diseases associated with fusion partners.
(DisGeNet 4.0)
PartnerGeneDisease IDDisease name# pubmedsSource
TgeneC0553580Ewings sarcoma3CTD_human;ORPHANET
TgeneC0002736Amyotrophic Lateral Sclerosis1GENOMICS_ENGLAND
TgeneC0033578Prostatic Neoplasms1CTD_human
TgeneC0206651Clear Cell Sarcoma of Soft Tissue1ORPHANET
TgeneC0206663Neuroectodermal Tumor, Primitive1CTD_human
TgeneC0279980Extra-osseous Ewing's sarcoma1ORPHANET
TgeneC0334584Spongioblastoma1CTD_human
TgeneC0334596Medulloepithelioma1CTD_human
TgeneC0376358Malignant neoplasm of prostate1CTD_human
TgeneC0700367Ependymoblastoma1CTD_human
TgeneC0751675Cerebral Primitive Neuroectodermal Tumor1CTD_human
TgeneC1275278Extraskeletal Myxoid Chondrosarcoma1ORPHANET