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Center for Computational Systems Medicine
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Fusion Gene Summary

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Fusion Gene ORF analysis

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Fusion Genomic Features

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Fusion Protein Features

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Fusion Gene Sequence

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Fusion Gene PPI analysis

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Related Drugs

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Related Diseases

Fusion gene:MYDGF-MAP2K3 (FusionGDB2 ID:HG56005TG5606)

Fusion Gene Summary for MYDGF-MAP2K3

check button Fusion gene summary
Fusion gene informationFusion gene name: MYDGF-MAP2K3
Fusion gene ID: hg56005tg5606
HgeneTgene
Gene symbol

MYDGF

MAP2K3

Gene ID

56005

5606

Gene namemyeloid derived growth factormitogen-activated protein kinase kinase 3
SynonymsC19orf10|EUROIMAGE1875335|IL25|IL27|IL27w|R33729_1|SF20MAPKK3|MEK3|MKK3|PRKMK3|SAPKK-2|SAPKK2
Cytomap('C19orf10','MYDGF')('MAP2K3','MAP2K3')

19p13.3

17p11.2

Type of geneprotein-codingprotein-coding
Descriptionmyeloid-derived growth factorUPF0556 protein C19orf10interleukin 27 working designationinterleukin-25stromal cell-derived growth factor SF20dual specificity mitogen-activated protein kinase kinase 3MAP kinase kinase 3MAPK/ERK kinase 3MAPKK 3MEK 3SAPK kinase 2stress-activated protein kinase kinase 2
Modification date2020031320200327
UniProtAcc..
Ensembl transtripts involved in fusion geneENST00000262947, ENST00000599630, 
Fusion gene scores* DoF score4 X 4 X 4=645 X 5 X 3=75
# samples 55
** MAII scorelog2(5/64*10)=-0.356143810225275
possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs).
DoF>8 and MAII<0
log2(5/75*10)=-0.584962500721156
possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs).
DoF>8 and MAII<0
Context

PubMed: MYDGF [Title/Abstract] AND MAP2K3 [Title/Abstract] AND fusion [Title/Abstract]

Most frequent breakpointC19orf10(4659942)-MAP2K3(21201724), # samples:1
MYDGF(4659943)-MAP2K3(21201725), # samples:1
Anticipated loss of major functional domain due to fusion event.C19orf10-MAP2K3 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF.
C19orf10-MAP2K3 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF.
* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

check button Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez
PartnerGeneGO IDGO termPubMed ID
HgeneMYDGF

GO:0001938

positive regulation of endothelial cell proliferation

25581518

HgeneMYDGF

GO:0045766

positive regulation of angiogenesis

25581518

TgeneMAP2K3

GO:0045860

positive regulation of protein kinase activity

11980910

TgeneMAP2K3

GO:0045893

positive regulation of transcription, DNA-templated

11980910


check buttonFusion gene breakpoints across C19orf10 (5'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.
all structure
check buttonFusion gene breakpoints across MAP2K3 (3'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.
all structure

check button Fusion gene information
* All genome coordinats were lifted-over on hg19.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.
SourceDiseaseSampleHgeneHchrHbpHstrandTgeneTchrTbpTstrand
ChimerDB4SARCTCGA-FX-A3TO-01AMYDGFchr19

4659943

-MAP2K3chr17

21201725

+
ChimerDB4SARCTCGA-FX-A3TOC19orf10chr19

4659942

-MAP2K3chr17

21201724

+


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Fusion Gene ORF analysis for MYDGF-MAP2K3

check button Open reading frame (ORF) analsis of fusion genes based on Ensembl gene isoform structure.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.
ORFHenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrand
5CDS-5UTRENST00000262947ENST00000316920C19orf10chr19

4659942

-MAP2K3chr17

21201724

+
5CDS-5UTRENST00000262947ENST00000361818C19orf10chr19

4659942

-MAP2K3chr17

21201724

+
5CDS-intronENST00000262947ENST00000534743C19orf10chr19

4659942

-MAP2K3chr17

21201724

+
In-frameENST00000262947ENST00000342679C19orf10chr19

4659942

-MAP2K3chr17

21201724

+
intron-3CDSENST00000599630ENST00000342679C19orf10chr19

4659942

-MAP2K3chr17

21201724

+
intron-5UTRENST00000599630ENST00000316920C19orf10chr19

4659942

-MAP2K3chr17

21201724

+
intron-5UTRENST00000599630ENST00000361818C19orf10chr19

4659942

-MAP2K3chr17

21201724

+
intron-intronENST00000599630ENST00000534743C19orf10chr19

4659942

-MAP2K3chr17

21201724

+

check buttonORFfinder result based on the fusion transcript sequence of in-frame fusion genes.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandSeq length
(transcript)
BP loci
(transcript)
Predicted start
(transcript)
Predicted stop
(transcript)
Seq length
(amino acids)
ENST00000262947C19orf10chr194659942-ENST00000342679MAP2K3chr1721201724+2483478361472478

check buttonDeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandNo-coding scoreCoding score
ENST00000262947ENST00000342679C19orf10chr194659942-MAP2K3chr1721201724+0.0035552430.99644476

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Fusion Genomic Features for MYDGF-MAP2K3


check buttonFusionAI prediction of the potential fusion gene breakpoint based on the pre-mature RNA sequence context (+/- 5kb of individual partner genes, total 20kb length sequence). FusionAI is a fusion gene breakpoint classifier based on convolutional neural network by comparing the fusion positive and negative sequence context of ~ 20K fusion gene data. From here, we can have the relative potentency of the 20K genomic sequence how individual sequnce will be likely used as the gene fusion breakpoints.
HgeneHchrHbpHstrandTgeneTchrTbpTstrand1-pp (fusion gene breakpoint)
C19orf10chr194659942-MAP2K3chr1721201724+0.0097956260.99020433
C19orf10chr194659942-MAP2K3chr1721201724+0.0097956260.99020433

check buttonDistribution of 44 human genomic features loci across 20kb length fusion breakpoint regions. We integrated a total of 44 different types of human genomic feature loci information across five big categories including virus integration sites, repeats, structural variants, chromatin states, and gene expression regulation. More details are in help page.
genomic feature

check buttonDistribution of 44 human genomic features loci across 20kb length fusion breakpoint regions that are ovelapped with the top 1% feature importance score regions. More details are in help page.
genomic feature of top 1%

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Fusion Protein Features for MYDGF-MAP2K3


check button Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/:4659942/:21201724)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
4. Go down and press 'Search' tab twice.
4. Go down to have the hyperlink of the search result.
5. Click the hyperlink.
6. See the FGviewer result for your fusion gene.
FGviewer

check buttonMain function of each fusion partner protein. (from UniProt)
HgeneTgene
..
FUNCTION: Transcriptional activator which is required for calcium-dependent dendritic growth and branching in cortical neurons. Recruits CREB-binding protein (CREBBP) to nuclear bodies. Component of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating a calcium-dependent release of a repressor complex and a recruitment of an activator complex. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex. At the same time, there is increased recruitment of CREBBP to the promoter by a CREST-dependent mechanism, which leads to transcriptional activation. The CREST-BRG1 complex also binds to the NR2B promoter, and activity-dependent induction of NR2B expression involves a release of HDAC1 and recruitment of CREBBP (By similarity). {ECO:0000250}.FUNCTION: Transcriptional activator which is required for calcium-dependent dendritic growth and branching in cortical neurons. Recruits CREB-binding protein (CREBBP) to nuclear bodies. Component of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating a calcium-dependent release of a repressor complex and a recruitment of an activator complex. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex. At the same time, there is increased recruitment of CREBBP to the promoter by a CREST-dependent mechanism, which leads to transcriptional activation. The CREST-BRG1 complex also binds to the NR2B promoter, and activity-dependent induction of NR2B expression involves a release of HDAC1 and recruitment of CREBBP (By similarity). {ECO:0000250}.

check buttonRetention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at

download page


* Minus value of BPloci means that the break pointn is located before the CDS.
- In-frame and retained protein feature among the 13 regional features.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note
TgeneMAP2K3chr19:4659942chr17:21201724ENST0000031692011364_3250319.0DomainProtein kinase
TgeneMAP2K3chr19:4659942chr17:21201724ENST0000034267901264_32516348.0DomainProtein kinase
TgeneMAP2K3chr19:4659942chr17:21201724ENST0000036181801264_3250319.0DomainProtein kinase
TgeneMAP2K3chr19:4659942chr17:21201724ENST0000031692011370_780319.0Nucleotide bindingATP
TgeneMAP2K3chr19:4659942chr17:21201724ENST0000034267901270_7816348.0Nucleotide bindingATP
TgeneMAP2K3chr19:4659942chr17:21201724ENST0000036181801270_780319.0Nucleotide bindingATP

- In-frame and not-retained protein feature among the 13 regional features.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note


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Fusion Gene Sequence for MYDGF-MAP2K3


check button For in-frame fusion transcripts, we provide the fusion transcript sequences and fusion amino acid sequences. To have fusion amino acid sequence, we ran ORFfinder and chose the longest ORF among the all predicted ones.
>11155_11155_1_C19orf10-MAP2K3_C19orf10_chr19_4659942_ENST00000262947_MAP2K3_chr17_21201724_ENST00000342679_length(transcript)=2483nt_BP=478nt
GTCTACCCGCCCCTGCCCTGAGGACCCTAGTCCAACATGGCGGCGCCCAGCGGAGGGTGGAACGGCGTCGGCGCGAGCTTGTGGGCCGCG
CTGCTCCTAGGGGCCGTGGCGCTGAGGCCGGCGGAGGCGGTGTCCGAGCCCACGACGGTGGCGTTTGACGTGCGGCCCGGCGGCGTCGTG
CATTCCTTCTCCCATAACGTGGGCCCGGGGGACAAATATACGTGTATGTTCACTTACGCCTCTCAAGGAGGGACCAATGAGCAATGGCAG
ATGAGTCTGGGGACCAGCGAAGACCACCAGCACTTCACCTGCACCATCTGGAGGCCCCAGGGGAAGTCCTATCTGTACTTCACACAGTTC
AAGGCAGAGGTGCGGGGCGCTGAGATTGAGTACGCCATGGCCTACTCTAAAGCCGCATTTGAAAGGGAAAGTGATGTCCCTCTGAAAACT
GAGGAATTTGAAGTGACCAAAACAGCAGGAAAATCCAAGAGGAAGAAGGATCTACGGATATCCTGCATGTCCAAGCCACCCGCACCCAAC
CCCACACCCCCCCGGAACCTGGACTCCCGGACCTTCATCACCATTGGAGACAGAAACTTTGAGGTGGAGGCTGATGACTTGGTGACCATC
TCAGAACTGGGCCGTGGAGCCTATGGGGTGGTAGAGAAGGTGCGGCACGCCCAGAGCGGCACCATCATGGCCGTGAAGCGGATCCGGGCC
ACCGTGAACTCACAGGAGCAGAAGCGGCTGCTCATGGACCTGGACATCAACATGCGCACGGTCGACTGTTTCTACACTGTCACCTTCTAC
GGGGCACTATTCAGAGAGGGAGACGTGTGGATCTGCATGGAGCTCATGGACACATCCTTGGACAAGTTCTACCGGAAGGTGCTGGATAAA
AACATGACAATTCCAGAGGACATCCTTGGGGAGATTGCTGTGTCTATCGTGCGGGCCCTGGAGCATCTGCACAGCAAGCTGTCGGTGATC
CACAGAGATGTGAAGCCCTCCAATGTCCTTATCAACAAGGAGGGCCATGTGAAGATGTGTGACTTTGGCATCAGTGGCTACTTGGTGGAC
TCTGTGGCCAAGACGATGGATGCCGGCTGCAAGCCCTACATGGCCCCTGAGAGGATCAACCCAGAGCTGAACCAGAAGGGCTACAATGTC
AAGTCCGACGTCTGGAGCCTGGGCATCACCATGATTGAGATGGCCATCCTGCGGTTCCCTTACGAGTCCTGGGGGACCCCGTTCCAGCAG
CTGAAGCAGGTGGTGGAGGAGCCGTCCCCCCAGCTCCCAGCCGACCGTTTCTCCCCCGAGTTTGTGGACTTCACTGCTCAGTGCCTGAGG
AAGAACCCCGCAGAGCGTATGAGCTACCTGGAGCTGATGGAGCACCCCTTCTTCACCTTGCACAAAACCAAGAAGACGGACATTGCTGCC
TTCGTGAAGGAGATCCTGGGAGAAGACTCATAGGGGCTGGGCCTCGGACCCCACTCCGGCCCTCCAGAGCCCCACAGCCCCATCTGCGGG
GGCAGTGCTCACCCACACCATAAGCTACTGCCATCCTGGCCCAGGGCATCTGGGAGGAACCGAGGGGGCTGCTCCCACCTGGCTCTGTGG
CGAGCCATTTGTCCCAAGTGCCAAAGAAGCAGACCATTGGGGCTCCCAGCCAGGCCCTTGTCGGCCCCACCAGTGCCTCTCCCTGCTGCT
CCTAGGACCCGTCTCCAGCTGCTGAGATCCTGGACTGAGGGGGCCTGGATGCCCCCTGTGGATGCTGCTGCCCCTGCACAGCAGGCTGCC
AGTGCCTGGGTGGATGGGCCACCGCCTTGCCCAGCCTGGATGCCATCCAAGTTGTATATTTTTTTAATCTCTCGACTGAATGGACTTTGC
ACACTTTGGCCCAGGGTGGCCACACCTCTATCCCGGCTTTGGTGCGGGGTACACAAGAGGGGATGAGTTGTGTGAATACCCCAAGACTCC
CATGAGGGAGATGCCATGAGCCGCCCAAGGCCTTCCCCTGGCACTGGCAAACAGGGCCTCTGCGGAGCACACTGGCTCACCCAGTCCTGC
CCGCCACCGTTATCGGTGTCATTCACCTTTCGTGTTTTTTTTAATTTATCCTCTGTTGATTTTTTCTTTTGCTTTATGGGTTTGGCTTGT
TTTTCTTGCATGGTTTGGAGCTGATCGCTTCTCCCCCACCCCCTAGGGTACCAGCAGGCAGAGCCTTGCCCTCTGCTCAGGCTGGGGTCC
AGTGGGAGGGGCCCAAGATCTCTGCTCAGAGAAGTGCAGGGGGAGCCTTCCAGCTCACTCTCCCTGAGGACTGGCTTGACAGGGGCTATG
GGTTTGCTTTGGTGTTGTTTTTAAAAAAAGAAAATATATTTTTTTGAAAAAACGACTGCCCATCCCGGGTCCTTTCCCTGATGGGTTGGG

>11155_11155_1_C19orf10-MAP2K3_C19orf10_chr19_4659942_ENST00000262947_MAP2K3_chr17_21201724_ENST00000342679_length(amino acids)=478AA_BP=147
MAAPSGGWNGVGASLWAALLLGAVALRPAEAVSEPTTVAFDVRPGGVVHSFSHNVGPGDKYTCMFTYASQGGTNEQWQMSLGTSEDHQHF
TCTIWRPQGKSYLYFTQFKAEVRGAEIEYAMAYSKAAFERESDVPLKTEEFEVTKTAGKSKRKKDLRISCMSKPPAPNPTPPRNLDSRTF
ITIGDRNFEVEADDLVTISELGRGAYGVVEKVRHAQSGTIMAVKRIRATVNSQEQKRLLMDLDINMRTVDCFYTVTFYGALFREGDVWIC
MELMDTSLDKFYRKVLDKNMTIPEDILGEIAVSIVRALEHLHSKLSVIHRDVKPSNVLINKEGHVKMCDFGISGYLVDSVAKTMDAGCKP
YMAPERINPELNQKGYNVKSDVWSLGITMIEMAILRFPYESWGTPFQQLKQVVEEPSPQLPADRFSPEFVDFTAQCLRKNPAERMSYLEL

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Fusion Gene PPI Analysis for MYDGF-MAP2K3


check button Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in

ChiPPI page.


check button Protein-protein interactors with each fusion partner protein in wild-type (BIOGRID-3.4.160)
HgeneHgene's interactorsTgeneTgene's interactors


check button - Retained PPIs in in-frame fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenStill interaction with


check button - Lost PPIs in in-frame fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenInteraction lost with


check button - Retained PPIs, but lost function due to frame-shift fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenInteraction lost with


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Related Drugs for MYDGF-MAP2K3


check button Drugs targeting genes involved in this fusion gene.
(DrugBank Version 5.1.8 2021-05-08)
PartnerGeneUniProtAccDrugBank IDDrug nameDrug activityDrug typeDrug status

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Related Diseases for MYDGF-MAP2K3


check button Diseases associated with fusion partners.
(DisGeNet 4.0)
PartnerGeneDisease IDDisease name# pubmedsSource
HgeneMYDGFC0022548Keloid1CTD_human