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 | Fusion Gene Summary |
| Fusion Gene ORF analysis |
| Fusion Genomic Features |
| Fusion Protein Features |
| Fusion Gene Sequence |
| Fusion Gene PPI analysis |
| Related Drugs |
| Related Diseases |
Fusion gene:GRK1-CUL4A (FusionGDB2 ID:HG6011TG8451) |
Fusion Gene Summary for GRK1-CUL4A |
 Fusion gene summary |
| Fusion gene information | Fusion gene name: GRK1-CUL4A | Fusion gene ID: hg6011tg8451 | Hgene | Tgene | Gene symbol | GRK1 | CUL4A | Gene ID | 6011 | 8451 |
| Gene name | G protein-coupled receptor kinase 1 | cullin 4A | |
| Synonyms | GPRK1|RHOK|RK | - | |
| Cytomap | ('GRK1')('CUL4A') 13q34 | 13q34 | |
| Type of gene | protein-coding | protein-coding | |
| Description | rhodopsin kinase GRK1rhodopsin kinase | cullin-4ACUL-4A | |
| Modification date | 20200313 | 20200327 | |
| UniProtAcc | . | Q13619 | |
| Ensembl transtripts involved in fusion gene | ENST00000335678, ENST00000545304, | ||
| Fusion gene scores | * DoF score | 1 X 1 X 1=1 | 15 X 13 X 9=1755 |
| # samples | 1 | 20 | |
| ** MAII score | log2(1/1*10)=3.32192809488736 | log2(20/1755*10)=-3.1333991254172 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0 | |
| Context | PubMed: GRK1 [Title/Abstract] AND CUL4A [Title/Abstract] AND fusion [Title/Abstract] | ||
| Most frequent breakpoint | GRK1(114321964)-CUL4A(113862913), # samples:1 | ||
| Anticipated loss of major functional domain due to fusion event. | |||
| * DoF score (Degree of Frequency) = # partners X # break points X # cancer types ** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10) |
| Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez |
| Partner | Gene | GO ID | GO term | PubMed ID |
| Hgene | GRK1 | GO:0022400 | regulation of rhodopsin mediated signaling pathway | 15946941 |
| Tgene | CUL4A | GO:0016567 | protein ubiquitination | 26431207 |
| Fusion gene information * All genome coordinats were lifted-over on hg19. * Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser. |
| Source | Disease | Sample | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand |
| ChimerDB4 | LUSC | TCGA-56-A4ZK | GRK1 | chr13 | 114321964 | + | CUL4A | chr13 | 113862913 | + |
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Fusion Gene ORF analysis for GRK1-CUL4A |
| Open reading frame (ORF) analsis of fusion genes based on Ensembl gene isoform structure. * Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser. |
| ORF | Henst | Tenst | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand |
| 5CDS-5UTR | ENST00000335678 | ENST00000375441 | GRK1 | chr13 | 114321964 | + | CUL4A | chr13 | 113862913 | + |
| 5CDS-intron | ENST00000335678 | ENST00000326335 | GRK1 | chr13 | 114321964 | + | CUL4A | chr13 | 113862913 | + |
| 5CDS-intron | ENST00000335678 | ENST00000375440 | GRK1 | chr13 | 114321964 | + | CUL4A | chr13 | 113862913 | + |
| 5CDS-intron | ENST00000335678 | ENST00000451881 | GRK1 | chr13 | 114321964 | + | CUL4A | chr13 | 113862913 | + |
| 5CDS-intron | ENST00000335678 | ENST00000463426 | GRK1 | chr13 | 114321964 | + | CUL4A | chr13 | 113862913 | + |
| intron-5UTR | ENST00000545304 | ENST00000375441 | GRK1 | chr13 | 114321964 | + | CUL4A | chr13 | 113862913 | + |
| intron-intron | ENST00000545304 | ENST00000326335 | GRK1 | chr13 | 114321964 | + | CUL4A | chr13 | 113862913 | + |
| intron-intron | ENST00000545304 | ENST00000375440 | GRK1 | chr13 | 114321964 | + | CUL4A | chr13 | 113862913 | + |
| intron-intron | ENST00000545304 | ENST00000451881 | GRK1 | chr13 | 114321964 | + | CUL4A | chr13 | 113862913 | + |
| intron-intron | ENST00000545304 | ENST00000463426 | GRK1 | chr13 | 114321964 | + | CUL4A | chr13 | 113862913 | + |
| ORFfinder result based on the fusion transcript sequence of in-frame fusion genes. |
| Henst | Tenst | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand | Seq length (transcript) | BP loci (transcript) | Predicted start (transcript) | Predicted stop (transcript) | Seq length (amino acids) |
| DeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated. |
| Henst | Tenst | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand | No-coding score | Coding score |
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Fusion Genomic Features for GRK1-CUL4A |
| FusionAI prediction of the potential fusion gene breakpoint based on the pre-mature RNA sequence context (+/- 5kb of individual partner genes, total 20kb length sequence). FusionAI is a fusion gene breakpoint classifier based on convolutional neural network by comparing the fusion positive and negative sequence context of ~ 20K fusion gene data. From here, we can have the relative potentency of the 20K genomic sequence how individual sequnce will be likely used as the gene fusion breakpoints. |
| Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand | 1-p | p (fusion gene breakpoint) |
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Fusion Protein Features for GRK1-CUL4A |
| Four levels of functional features of fusion genes Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/:114321964/:113862913) - FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels. - How to search 1. Put your fusion gene symbol. 2. Press the tab key until there will be shown the breakpoint information filled. 4. Go down and press 'Search' tab twice. 4. Go down to have the hyperlink of the search result. 5. Click the hyperlink. 6. See the FGviewer result for your fusion gene. |
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| Main function of each fusion partner protein. (from UniProt) |
| Hgene | Tgene |
| . | CUL4A |
| FUNCTION: Transcriptional activator which is required for calcium-dependent dendritic growth and branching in cortical neurons. Recruits CREB-binding protein (CREBBP) to nuclear bodies. Component of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating a calcium-dependent release of a repressor complex and a recruitment of an activator complex. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex. At the same time, there is increased recruitment of CREBBP to the promoter by a CREST-dependent mechanism, which leads to transcriptional activation. The CREST-BRG1 complex also binds to the NR2B promoter, and activity-dependent induction of NR2B expression involves a release of HDAC1 and recruitment of CREBBP (By similarity). {ECO:0000250}. | FUNCTION: Core component of multiple cullin-RING-based E3 ubiquitin-protein ligase complexes which mediate the ubiquitination of target proteins. As a scaffold protein may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and is inhibited by the association of the deneddylated cullin subunit with TIP120A/CAND1. The functional specificity of the E3 ubiquitin-protein ligase complex depends on the variable substrate recognition component. DCX(DET1-COP1) directs ubiquitination of JUN. DCX(DDB2) directs ubiquitination of XPC. DCX(DDB2) ubiquitinates histones H3-H4 and is required for efficient histone deposition during replication-coupled (H3.1) and replication-independent (H3.3) nucleosome assembly, probably by facilitating the transfer of H3 from ASF1A/ASF1B to other chaperones involved in histone deposition. DCX(DTL) plays a role in PCNA-dependent polyubiquitination of CDT1 and MDM2-dependent ubiquitination of TP53 in response to radiation-induced DNA damage and during DNA replication. In association with DDB1 and SKP2 probably is involved in ubiquitination of CDKN1B/p27kip. Is involved in ubiquitination of HOXA9. DCX(DTL) directs autoubiquitination of DTL. The DDB1-CUL4A-DTL E3 ligase complex regulates the circadian clock function by mediating the ubiquitination and degradation of CRY1 (PubMed:26431207). With CUL4B, contributes to ribosome biogenesis (PubMed:26711351). {ECO:0000269|PubMed:14578910, ECO:0000269|PubMed:14609952, ECO:0000269|PubMed:15448697, ECO:0000269|PubMed:15548678, ECO:0000269|PubMed:16537899, ECO:0000269|PubMed:16678110, ECO:0000269|PubMed:23478445, ECO:0000269|PubMed:24209620, ECO:0000269|PubMed:26431207, ECO:0000269|PubMed:26711351}. |
| Retention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at * Minus value of BPloci means that the break pointn is located before the CDS. |
| - In-frame and retained protein feature among the 13 regional features. |
| Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Protein feature | Protein feature note |
| - In-frame and not-retained protein feature among the 13 regional features. |
| Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Protein feature | Protein feature note |
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Fusion Gene Sequence for GRK1-CUL4A |
| For in-frame fusion transcripts, we provide the fusion transcript sequences and fusion amino acid sequences. To have fusion amino acid sequence, we ran ORFfinder and chose the longest ORF among the all predicted ones. |
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Fusion Gene PPI Analysis for GRK1-CUL4A |
| Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in |
| Protein-protein interactors with each fusion partner protein in wild-type (BIOGRID-3.4.160) |
| Hgene | Hgene's interactors | Tgene | Tgene's interactors |
| - Retained PPIs in in-frame fusion. |
| Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Still interaction with |
| - Lost PPIs in in-frame fusion. |
| Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Interaction lost with |
| - Retained PPIs, but lost function due to frame-shift fusion. |
| Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Interaction lost with |
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Related Drugs for GRK1-CUL4A |
| Drugs targeting genes involved in this fusion gene. (DrugBank Version 5.1.8 2021-05-08) |
| Partner | Gene | UniProtAcc | DrugBank ID | Drug name | Drug activity | Drug type | Drug status |
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Related Diseases for GRK1-CUL4A |
| Diseases associated with fusion partners. (DisGeNet 4.0) |
| Partner | Gene | Disease ID | Disease name | # pubmeds | Source |
| Hgene | GRK1 | C1306122 | Oguchi disease | 2 | CTD_human;ORPHANET |
| Hgene | GRK1 | C3150678 | OGUCHI DISEASE 2 | 2 | GENOMICS_ENGLAND;UNIPROT |
| Tgene | C0004238 | Atrial Fibrillation | 2 | CTD_human | |
| Tgene | C0235480 | Paroxysmal atrial fibrillation | 2 | CTD_human | |
| Tgene | C2585653 | Persistent atrial fibrillation | 2 | CTD_human | |
| Tgene | C3468561 | familial atrial fibrillation | 2 | CTD_human | |
| Tgene | C0014518 | Toxic Epidermal Necrolysis | 1 | CTD_human | |
| Tgene | C0038325 | Stevens-Johnson Syndrome | 1 | CTD_human | |
| Tgene | C1274933 | Drug-Induced Stevens Johnson Syndrome | 1 | CTD_human | |
| Tgene | C3658301 | Mycoplasma-Induced Stevens-Johnson Syndrome | 1 | CTD_human | |
| Tgene | C3658302 | Stevens-Johnson Syndrome Toxic Epidermal Necrolysis Spectrum | 1 | CTD_human |
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