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in Kim Lab

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Center for Computational Systems Medicine
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Fusion Gene Summary

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Fusion Gene ORF analysis

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Fusion Genomic Features

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Fusion Protein Features

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Fusion Gene Sequence

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Fusion Gene PPI analysis

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Related Drugs

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Related Diseases

Fusion gene:RFWD2-AKT3 (FusionGDB2 ID:HG64326TG10000)

Fusion Gene Summary for RFWD2-AKT3

check button Fusion gene summary
Fusion gene informationFusion gene name: RFWD2-AKT3
Fusion gene ID: hg64326tg10000
HgeneTgene
Gene symbol

RFWD2

AKT3

Gene ID

64326

10000

Gene nameCOP1 E3 ubiquitin ligaseAKT serine/threonine kinase 3
SynonymsCFAP78|FAP78|RFWD2|RNF200MPPH|MPPH2|PKB-GAMMA|PKBG|PRKBG|RAC-PK-gamma|RAC-gamma|STK-2
Cytomap('RFWD2')('AKT3')

1q25.1-q25.2

1q43-q44

Type of geneprotein-codingprotein-coding
DescriptionE3 ubiquitin-protein ligase COP1E3 ubiquitin-protein ligase RFWD2RING finger protein 200RING-type E3 ubiquitin transferase RFWD2constitutive photomorphogenesis protein 1 homologconstitutive photomorphogenic protein 1putative ubiquitin ligase COP1riRAC-gamma serine/threonine-protein kinasePKB gammaRAC-gamma serine/threonine protein kinasev-akt murine thymoma viral oncogene homolog 3 (protein kinase B, gamma)
Modification date2020031320200313
UniProtAcc..
Ensembl transtripts involved in fusion geneENST00000308769, ENST00000367669, 
Fusion gene scores* DoF score17 X 15 X 10=255019 X 18 X 8=2736
# samples 2019
** MAII scorelog2(20/2550*10)=-3.6724253419715
possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs).
DoF>8 and MAII<0		log2(19/2736*10)=-3.84799690655495
possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs).
DoF>8 and MAII<0
Context

PubMed: RFWD2 [Title/Abstract] AND AKT3 [Title/Abstract] AND fusion [Title/Abstract]

Most frequent breakpointRFWD2(175956078)-AKT3(243727150), # samples:1
Anticipated loss of major functional domain due to fusion event.RFWD2-AKT3 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF.
RFWD2-AKT3 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF.
RFWD2-AKT3 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF.
RFWD2-AKT3 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF.
* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

check button Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez
PartnerGeneGO IDGO termPubMed ID
HgeneRFWD2

GO:0010212

response to ionizing radiation

19805145

TgeneAKT3

GO:0043536

positive regulation of blood vessel endothelial cell migration

28254819

TgeneAKT3

GO:1905564

positive regulation of vascular endothelial cell proliferation

28254819


check buttonFusion gene breakpoints across RFWD2 (5'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.
all structure
check buttonFusion gene breakpoints across AKT3 (3'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.
all structure

check button Fusion gene information
* All genome coordinats were lifted-over on hg19.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.
SourceDiseaseSampleHgeneHchrHbpHstrandTgeneTchrTbpTstrand
ChimerDB4STADTCGA-D7-A4Z0RFWD2chr1

175956078

-AKT3chr1

243727150

-


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Fusion Gene ORF analysis for RFWD2-AKT3

check button Open reading frame (ORF) analsis of fusion genes based on Ensembl gene isoform structure.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.
ORFHenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrand
5CDS-intronENST00000308769ENST00000492957RFWD2chr1

175956078

-AKT3chr1

243727150

-
5CDS-intronENST00000367669ENST00000492957RFWD2chr1

175956078

-AKT3chr1

243727150

-
In-frameENST00000308769ENST00000263826RFWD2chr1

175956078

-AKT3chr1

243727150

-
In-frameENST00000308769ENST00000336199RFWD2chr1

175956078

-AKT3chr1

243727150

-
In-frameENST00000308769ENST00000366539RFWD2chr1

175956078

-AKT3chr1

243727150

-
In-frameENST00000308769ENST00000366540RFWD2chr1

175956078

-AKT3chr1

243727150

-
In-frameENST00000367669ENST00000263826RFWD2chr1

175956078

-AKT3chr1

243727150

-
In-frameENST00000367669ENST00000336199RFWD2chr1

175956078

-AKT3chr1

243727150

-
In-frameENST00000367669ENST00000366539RFWD2chr1

175956078

-AKT3chr1

243727150

-
In-frameENST00000367669ENST00000366540RFWD2chr1

175956078

-AKT3chr1

243727150

-

check buttonORFfinder result based on the fusion transcript sequence of in-frame fusion genes.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandSeq length
(transcript)		BP loci
(transcript)		Predicted start
(transcript)		Predicted stop
(transcript)		Seq length
(amino acids)
ENST00000367669RFWD2chr1175956078-ENST00000336199AKT3chr1243727150-345126485153226903
ENST00000367669RFWD2chr1175956078-ENST00000366540AKT3chr1243727150-325126485153226903
ENST00000367669RFWD2chr1175956078-ENST00000366539AKT3chr1243727150-675526485153268917
ENST00000367669RFWD2chr1175956078-ENST00000263826AKT3chr1243727150-533626485153268917
ENST00000308769RFWD2chr1175956078-ENST00000336199AKT3chr1243727150-2864206102639879
ENST00000308769RFWD2chr1175956078-ENST00000366540AKT3chr1243727150-2664206102639879
ENST00000308769RFWD2chr1175956078-ENST00000366539AKT3chr1243727150-6168206102681893
ENST00000308769RFWD2chr1175956078-ENST00000263826AKT3chr1243727150-4749206102681893

check buttonDeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandNo-coding scoreCoding score
ENST00000367669ENST00000336199RFWD2chr1175956078-AKT3chr1243727150-0.0006990140.99930096
ENST00000367669ENST00000366540RFWD2chr1175956078-AKT3chr1243727150-0.0006434140.99935657
ENST00000367669ENST00000366539RFWD2chr1175956078-AKT3chr1243727150-0.0004325050.99956745
ENST00000367669ENST00000263826RFWD2chr1175956078-AKT3chr1243727150-0.0006278560.9993722
ENST00000308769ENST00000336199RFWD2chr1175956078-AKT3chr1243727150-0.0007013180.9992987
ENST00000308769ENST00000366540RFWD2chr1175956078-AKT3chr1243727150-0.0006561950.9993438
ENST00000308769ENST00000366539RFWD2chr1175956078-AKT3chr1243727150-0.0004278270.9995722
ENST00000308769ENST00000263826RFWD2chr1175956078-AKT3chr1243727150-0.0006145660.99938536

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Fusion Genomic Features for RFWD2-AKT3


check buttonFusionAI prediction of the potential fusion gene breakpoint based on the pre-mature RNA sequence context (+/- 5kb of individual partner genes, total 20kb length sequence). FusionAI is a fusion gene breakpoint classifier based on convolutional neural network by comparing the fusion positive and negative sequence context of ~ 20K fusion gene data. From here, we can have the relative potentency of the 20K genomic sequence how individual sequnce will be likely used as the gene fusion breakpoints.
HgeneHchrHbpHstrandTgeneTchrTbpTstrand1-pp (fusion gene breakpoint)

check buttonDistribution of 44 human genomic features loci across 20kb length fusion breakpoint regions. We integrated a total of 44 different types of human genomic feature loci information across five big categories including virus integration sites, repeats, structural variants, chromatin states, and gene expression regulation. More details are in help page.
genomic feature

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Fusion Protein Features for RFWD2-AKT3


check button Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chr1:175956078/chr1:243727150)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
4. Go down and press 'Search' tab twice.
4. Go down to have the hyperlink of the search result.
5. Click the hyperlink.
6. See the FGviewer result for your fusion gene.
FGviewer

check buttonMain function of each fusion partner protein. (from UniProt)
HgeneTgene
..
FUNCTION: Transcriptional activator which is required for calcium-dependent dendritic growth and branching in cortical neurons. Recruits CREB-binding protein (CREBBP) to nuclear bodies. Component of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating a calcium-dependent release of a repressor complex and a recruitment of an activator complex. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex. At the same time, there is increased recruitment of CREBBP to the promoter by a CREST-dependent mechanism, which leads to transcriptional activation. The CREST-BRG1 complex also binds to the NR2B promoter, and activity-dependent induction of NR2B expression involves a release of HDAC1 and recruitment of CREBBP (By similarity). {ECO:0000250}.FUNCTION: Transcriptional activator which is required for calcium-dependent dendritic growth and branching in cortical neurons. Recruits CREB-binding protein (CREBBP) to nuclear bodies. Component of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating a calcium-dependent release of a repressor complex and a recruitment of an activator complex. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex. At the same time, there is increased recruitment of CREBBP to the promoter by a CREST-dependent mechanism, which leads to transcriptional activation. The CREST-BRG1 complex also binds to the NR2B promoter, and activity-dependent induction of NR2B expression involves a release of HDAC1 and recruitment of CREBBP (By similarity). {ECO:0000250}.

check buttonRetention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at

download page


* Minus value of BPloci means that the break pointn is located before the CDS.
- In-frame and retained protein feature among the 13 regional features.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note
HgeneRFWD2chr1:175956078chr1:243727150ENST00000308769-1719233_301687708.0Coiled coilOntology_term=ECO:0000255
HgeneRFWD2chr1:175956078chr1:243727150ENST00000367669-1820233_301711732.0Coiled coilOntology_term=ECO:0000255
HgeneRFWD2chr1:175956078chr1:243727150ENST00000308769-17192_110687708.0Compositional biasNote=Ser-rich
HgeneRFWD2chr1:175956078chr1:243727150ENST00000367669-18202_110711732.0Compositional biasNote=Ser-rich
HgeneRFWD2chr1:175956078chr1:243727150ENST00000308769-1719109_113687708.0MotifNote=Nuclear localization signal 1
HgeneRFWD2chr1:175956078chr1:243727150ENST00000308769-1719195_206687708.0MotifNote=Nuclear localization signal 2
HgeneRFWD2chr1:175956078chr1:243727150ENST00000308769-1719235_245687708.0MotifNuclear export signal
HgeneRFWD2chr1:175956078chr1:243727150ENST00000367669-1820109_113711732.0MotifNote=Nuclear localization signal 1
HgeneRFWD2chr1:175956078chr1:243727150ENST00000367669-1820195_206711732.0MotifNote=Nuclear localization signal 2
HgeneRFWD2chr1:175956078chr1:243727150ENST00000367669-1820235_245711732.0MotifNuclear export signal
HgeneRFWD2chr1:175956078chr1:243727150ENST00000308769-1719419_458687708.0RepeatNote=WD 1
HgeneRFWD2chr1:175956078chr1:243727150ENST00000308769-1719468_508687708.0RepeatNote=WD 2
HgeneRFWD2chr1:175956078chr1:243727150ENST00000308769-1719511_551687708.0RepeatNote=WD 3
HgeneRFWD2chr1:175956078chr1:243727150ENST00000308769-1719553_593687708.0RepeatNote=WD 4
HgeneRFWD2chr1:175956078chr1:243727150ENST00000308769-1719597_635687708.0RepeatNote=WD 5
HgeneRFWD2chr1:175956078chr1:243727150ENST00000308769-1719638_677687708.0RepeatNote=WD 6
HgeneRFWD2chr1:175956078chr1:243727150ENST00000367669-1820419_458711732.0RepeatNote=WD 1
HgeneRFWD2chr1:175956078chr1:243727150ENST00000367669-1820468_508711732.0RepeatNote=WD 2
HgeneRFWD2chr1:175956078chr1:243727150ENST00000367669-1820511_551711732.0RepeatNote=WD 3
HgeneRFWD2chr1:175956078chr1:243727150ENST00000367669-1820553_593711732.0RepeatNote=WD 4
HgeneRFWD2chr1:175956078chr1:243727150ENST00000367669-1820597_635711732.0RepeatNote=WD 5
HgeneRFWD2chr1:175956078chr1:243727150ENST00000367669-1820638_677711732.0RepeatNote=WD 6
HgeneRFWD2chr1:175956078chr1:243727150ENST00000308769-1719136_174687708.0Zinc fingerRING-type
HgeneRFWD2chr1:175956078chr1:243727150ENST00000367669-1820136_174711732.0Zinc fingerRING-type
TgeneAKT3chr1:175956078chr1:243727150ENST00000263826713406_479273480.0DomainAGC-kinase C-terminal
TgeneAKT3chr1:175956078chr1:243727150ENST00000336199714406_479273476.0DomainAGC-kinase C-terminal
TgeneAKT3chr1:175956078chr1:243727150ENST00000366539814406_479273480.0DomainAGC-kinase C-terminal
TgeneAKT3chr1:175956078chr1:243727150ENST00000366540814406_479273466.0DomainAGC-kinase C-terminal

- In-frame and not-retained protein feature among the 13 regional features.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note
HgeneRFWD2chr1:175956078chr1:243727150ENST00000308769-1719691_729687708.0RepeatNote=WD 7
HgeneRFWD2chr1:175956078chr1:243727150ENST00000367669-1820691_729711732.0RepeatNote=WD 7
TgeneAKT3chr1:175956078chr1:243727150ENST00000263826713148_405273480.0DomainProtein kinase
TgeneAKT3chr1:175956078chr1:243727150ENST000002638267135_107273480.0DomainPH
TgeneAKT3chr1:175956078chr1:243727150ENST00000336199714148_405273476.0DomainProtein kinase
TgeneAKT3chr1:175956078chr1:243727150ENST000003361997145_107273476.0DomainPH
TgeneAKT3chr1:175956078chr1:243727150ENST00000366539814148_405273480.0DomainProtein kinase
TgeneAKT3chr1:175956078chr1:243727150ENST000003665398145_107273480.0DomainPH
TgeneAKT3chr1:175956078chr1:243727150ENST00000366540814148_405273466.0DomainProtein kinase
TgeneAKT3chr1:175956078chr1:243727150ENST000003665408145_107273466.0DomainPH
TgeneAKT3chr1:175956078chr1:243727150ENST00000263826713154_162273480.0Nucleotide bindingATP
TgeneAKT3chr1:175956078chr1:243727150ENST00000336199714154_162273476.0Nucleotide bindingATP
TgeneAKT3chr1:175956078chr1:243727150ENST00000366539814154_162273480.0Nucleotide bindingATP
TgeneAKT3chr1:175956078chr1:243727150ENST00000366540814154_162273466.0Nucleotide bindingATP


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Fusion Gene Sequence for RFWD2-AKT3


check button For in-frame fusion transcripts, we provide the fusion transcript sequences and fusion amino acid sequences. To have fusion amino acid sequence, we ran ORFfinder and chose the longest ORF among the all predicted ones.
>73668_73668_1_RFWD2-AKT3_RFWD2_chr1_175956078_ENST00000308769_AKT3_chr1_243727150_ENST00000263826_length(transcript)=4749nt_BP=2061nt
ATGTCTGGTAGCCGCCAGGCCGGGTCGGGCTCCGCTGGGACAAGCCCCGGGTCCTCGGCGGCCTCCTCGGTGACTTCCGCCTCCTCGTCT
TTATCCTCTTCCCCGTCGCCGCCTTCCGTGGCGGTTTCGGCGGCAGCGCTGGTGTCCGGCGGGGTGGCCCAGGCCGCCGGCTCGGGCGGC
CTCGGGGGCCCGGTGCGGCCTGTGTTGGTGGCGCCCGCCGTATCGGGTAGCGGCGGCGGGGCGGTGTCCACGGGCCTGTCCCGGCACAGC
TGCGCGGCCAGGCCCAGCGCCGGCGTAGGAGGCAGCAGCTCCAGCCTAGGCAGCGGCAGCAGGAAGCGACCTCTCCTCGCCCCCCTCTGC
AACGGGCTCATCAACTCCTACGAGGACAAAAGCAACGACTTCGTATGCCCCATCTGCTTTGATATGATTGAAGAAGCATACATGACAAAA
TGTGGCCACAGCTTTTGCTACAAGTGTATTCATCAGAGTTTGGAGGACAATAATAGATGTCCCAAGTGTAACTATGTTGTGGACAATATT
GACCATCTGTATCCTAATTTCTTGGTGAATGAACTCATTCTTAAACAGAAGCAAAGATTTGAGGAAAAGAGGTTCAAATTGGACCACTCA
AATGGCCACAGGTGGCAGATATTTCAAGATTGGTTGGGAACTGACCAAGATAACCTTGATTTGGCCAATGTCAATCTTATGTTGGAGTTA
CTAGTGCAGAAGAAGAAACAACTGGAAGCAGAATCACATGCAGCCCAACTACAGATTCTTATGGAATTCCTCAAGGTTGCAAGAAGAAAT
AAGAGAGAGGAAATGAGTGGCTTATACTCTCCTGTCAGTGAGGATAGCACAGTGCCTCAATTTGAAGCTCCTTCTCCATCACACAGTAGT
ATTATTGATTCCACAGAATACAGCCAACCTCCAGGTTTCAGTGGCAGTTCTCAGACAAAGAAACAGCCTTGGTATAATAGCACGTTAGCA
TCAAGACGAAAACGACTTACTGCTCATTTTGAAGACTTGGAGCAGTGTTACTTTTCTACAAGGATGTCTCGTATCTCAGATGACAGTCGA
ACTGCAAGCCAGTTGGATGAATTTCAGGAATGCTTGTCCAAGTTTACTCGATATAATTCAGTACGACCTTTAGCCACATTGTCATATGCT
AGTGATCTCTATAATGGTTCCAGTATAGTCTCTAGTATTGAATTTGACCGGGATTGTGACTATTTTGCGATTGCTGGAGTTACAAAGAAG
ATTAAAGTCTATGAATATGACACTGTCATCCAGGATGCAGTGGATATTCATTACCCTGAGAATGAAATGACCTGCAATTCGAAAATCAGC
TGTATCAGTTGGAGTAGTTACCATAAGAACCTGTTAGCTAGCAGTGATTATGAAGGCACTGTTATTTTATGGGATGGATTCACAGGACAG
AGGTCAAAGGTCTATCAGGAGCATGAGAAGAGGTGTTGGAGTGTTGACTTTAATTTGATGGATCCTAAACTCTTGGCTTCAGGTTCTGAT
GATGCAAAAGTGAAGCTGTGGTCTACCAATCTAGACAACTCAGTGGCAAGCATTGAGGCAAAGGCTAATGTGTGCTGTGTTAAATTCAGC
CCCTCTTCCAGATACCATTTGGCTTTCGGCTGTGCAGATCACTGTGTCCACTACTATGATCTTCGTAACACTAAACAGCCAATCATGGTA
TTCAAAGGACACCGTAAAGCAGTCTCTTATGCAAAGTTTGTGAGTGGTGAGGAAATTGTCTCTGCCTCAACAGACAGTCAGCTAAAACTG
TGGAATGTAGGGAAACCATACTGCCTACGTTCCTTCAAGGGTCATATCAATGAAAAAAACTTTGTAGGCCTGGCTTCCAATGGAGATTAT
ATAGCTTGTGGAAGTGAAAATAACTCTCTCTACCTGTACTATAAAGGACTTTCTAAGACTTTGCTAACTTTTAAGTTTGATACAGTCAAA
AGTGTTCTCGACAAAGACCGAAAAGAAGATGATACAAATGAATTTGTTAGTGCTGTGTGCTGGAGGGCACTACCAGATGGGTTGGAGAAT
CTAATGCTGGACAAAGATGGCCACATAAAAATTACAGATTTTGGACTTTGCAAAGAAGGGATCACAGATGCAGCCACCATGAAGACATTC
TGTGGCACTCCAGAATATCTGGCACCAGAGGTGTTAGAAGATAATGACTATGGCCGAGCAGTAGACTGGTGGGGCCTAGGGGTTGTCATG
TATGAAATGATGTGTGGGAGGTTACCTTTCTACAACCAGGACCATGAGAAACTTTTTGAATTAATATTAATGGAAGACATTAAATTTCCT
CGAACACTCTCTTCAGATGCAAAATCATTGCTTTCAGGGCTCTTGATAAAGGATCCAAATAAACGCCTTGGTGGAGGACCAGATGATGCA
AAAGAAATTATGAGACACAGTTTCTTCTCTGGAGTAAACTGGCAAGATGTATATGATAAAAAGCTTGTACCTCCTTTTAAACCTCAAGTA
ACATCTGAGACAGATACTAGATATTTTGATGAAGAATTTACAGCTCAGACTATTACAATAACACCACCTGAAAAATATGATGAGGATGGT
ATGGACTGCATGGACAATGAGAGGCGGCCGCATTTCCCTCAATTTTCCTACTCTGCAAGTGGACGAGAATAAGTCTCTTTCATTCTGCTA
CTTCACTGTCATCTTCAATTTATTACTGAAAATGATTCCTGGACATCACCAGTCCTAGCTCTTACACATAGCAGGGGCACCTTCCGACAT
CCCAGACCAGCCAAGGGTCCTCACCCCTCGCCACCTTTCACCCTCATGAAAACACACATACACGCAAATACACTCCAGTTTTTGTTTTTG
CATGAAATTGTATCTCAGTCTAAGGTCTCATGCTGTTGCTGCTACTGTCTTACTATTATAGCAACTTTAAGAAGTAATTTTCCAACCTTT
GGAAGTCATGAGCCCACCATTGTTCATTTGTGCACCAATTATCATCTTTTGATCTTTTAGTTTTTCCCTCAGTGAAGGCTAAATGAGATA
CACTGATTCTAGGTACATTTTTTAACTTTCTAGAAGAGAAAAACTAACTAGACTAAGAAGATTTAGTTTATAAATTCAGAACAAGCAATT
GTGGAAGGGTGGTGGCGTGCATATGTAAAGCACATCAGATCCGTGCGTGAAGTAGGCATATATCACTAAGCTGTGGCTGGAATTGATTAG
GAAGCATTTGGTAGAAGGACTGAACAACTGTTGGGATATATATATATATATATAATTTTTTTTTTTTAAATTCCTGGTGGATACTGTAGA
AGAAGCCCATATCACATGTGGATGTCGAGACTTCACGGGCAATCATGAGCAAGTGAACACTGTTCTACCAAGAACTGAAGGCATATGCAC
AGTCAAGGTCACTTAAAGGGTCTTATGAAACAATTTGAGCCAGAGAGCATCTTTCCCCTGTGCTTGGAAACCTTTTTTCCTTCTTGACAT
TTATCACCTCTGATGGCTGAAGAATGTAGACAGGTATAATGATACTGCTTTTCACCAAAATTTCTACACCAAGGTAAACAGGTGTTTGCC
TTATTTAATTTTTTACTTTCAGTTCTACGTGAATTAGCTTTTTCTCAGATGTTGAAACTTTGAATGTCCTTTTATGATTTTGTTTATATT
GCAGTAGTATTTATTTTTTAGTGATGAGAATTGTATGTCATGTTAGCAAACGCAGCTCCAACTTATATAAAATAGACTTACTGCAGTTAC
TTTTGACCCATGTGCAAGGATTGTACACGCTGATGAGAATCATGCACTTTTTCTCCTCTGTTAAAAAAAATGATAAGGCTCTGAAATGGA
ATATATTGGTTAGAATTTGGCTTTGGGAGAAGAGATGCTGCCATTTAACCCCTTGGTACTGAAAATGAGAAAATCCCCAACTATGCATGC
CAAGGGGTTAATGAAACAAATAGCTGTTGACGTTTGCTCATTTAAGAATTTGAAACGTTATGATGACCTGGCAACAAAAAGTAATGAAGA
AAATTGAGACCTGAGTGAAGATAAGAAATGATCTTTACGTGGCAAAATGAACACATCTTGAGTATTTAGGAAATGGGCAGTGAAGGCTAA
GAACCTGGTGTGTTTCTTGGGATCATGGTACATTTATCACTGAATTAAGCCATCAGGGAAAAAACAACAAAAAAAGAGAACACCTCCAGC
TTTTCTTTTTCTGTATATACTCATGTCCCCCAGATTCCAACATTTCTCACTGAAAGGGGGCATGTATGCAAACCTCATCTTTCTCCTTCA
TTAATGATGATCTTCAGATTAAACCCTTTGGTGCTAGGAGCTGACAATTTCCAAAGCAGCCTGTGAAGTCCTAGGGGCTGGGGGCCACTC
TTGCGGCAAGCAGAAGGCCATCCTACTCCGCGGAGTGATCATGGAAATGTATTTTAGTTAAACTCTGACAGCTCCCAAACGGAAGACTAC
AGCATGACGTAGTATTATGATTGCATTGTATGAAAGAGCAAGTGACTTTCTAAGTAGGATGAATCATATTCATATGCAGATGTCTTAGCC
TCTTGACGCTGGAAGTGTGGATTTATAGCTATGAAACCACTGCTGGCAGTGGGTGGGCCACTGGGACTGACGGGGGTTAAAGGGCATTTT

>73668_73668_1_RFWD2-AKT3_RFWD2_chr1_175956078_ENST00000308769_AKT3_chr1_243727150_ENST00000263826_length(amino acids)=893AA_BP=687
MSGSRQAGSGSAGTSPGSSAASSVTSASSSLSSSPSPPSVAVSAAALVSGGVAQAAGSGGLGGPVRPVLVAPAVSGSGGGAVSTGLSRHS
CAARPSAGVGGSSSSLGSGSRKRPLLAPLCNGLINSYEDKSNDFVCPICFDMIEEAYMTKCGHSFCYKCIHQSLEDNNRCPKCNYVVDNI
DHLYPNFLVNELILKQKQRFEEKRFKLDHSNGHRWQIFQDWLGTDQDNLDLANVNLMLELLVQKKKQLEAESHAAQLQILMEFLKVARRN
KREEMSGLYSPVSEDSTVPQFEAPSPSHSSIIDSTEYSQPPGFSGSSQTKKQPWYNSTLASRRKRLTAHFEDLEQCYFSTRMSRISDDSR
TASQLDEFQECLSKFTRYNSVRPLATLSYASDLYNGSSIVSSIEFDRDCDYFAIAGVTKKIKVYEYDTVIQDAVDIHYPENEMTCNSKIS
CISWSSYHKNLLASSDYEGTVILWDGFTGQRSKVYQEHEKRCWSVDFNLMDPKLLASGSDDAKVKLWSTNLDNSVASIEAKANVCCVKFS
PSSRYHLAFGCADHCVHYYDLRNTKQPIMVFKGHRKAVSYAKFVSGEEIVSASTDSQLKLWNVGKPYCLRSFKGHINEKNFVGLASNGDY
IACGSENNSLYLYYKGLSKTLLTFKFDTVKSVLDKDRKEDDTNEFVSAVCWRALPDGLENLMLDKDGHIKITDFGLCKEGITDAATMKTF
CGTPEYLAPEVLEDNDYGRAVDWWGLGVVMYEMMCGRLPFYNQDHEKLFELILMEDIKFPRTLSSDAKSLLSGLLIKDPNKRLGGGPDDA

--------------------------------------------------------------
>73668_73668_2_RFWD2-AKT3_RFWD2_chr1_175956078_ENST00000308769_AKT3_chr1_243727150_ENST00000336199_length(transcript)=2864nt_BP=2061nt
ATGTCTGGTAGCCGCCAGGCCGGGTCGGGCTCCGCTGGGACAAGCCCCGGGTCCTCGGCGGCCTCCTCGGTGACTTCCGCCTCCTCGTCT
TTATCCTCTTCCCCGTCGCCGCCTTCCGTGGCGGTTTCGGCGGCAGCGCTGGTGTCCGGCGGGGTGGCCCAGGCCGCCGGCTCGGGCGGC
CTCGGGGGCCCGGTGCGGCCTGTGTTGGTGGCGCCCGCCGTATCGGGTAGCGGCGGCGGGGCGGTGTCCACGGGCCTGTCCCGGCACAGC
TGCGCGGCCAGGCCCAGCGCCGGCGTAGGAGGCAGCAGCTCCAGCCTAGGCAGCGGCAGCAGGAAGCGACCTCTCCTCGCCCCCCTCTGC
AACGGGCTCATCAACTCCTACGAGGACAAAAGCAACGACTTCGTATGCCCCATCTGCTTTGATATGATTGAAGAAGCATACATGACAAAA
TGTGGCCACAGCTTTTGCTACAAGTGTATTCATCAGAGTTTGGAGGACAATAATAGATGTCCCAAGTGTAACTATGTTGTGGACAATATT
GACCATCTGTATCCTAATTTCTTGGTGAATGAACTCATTCTTAAACAGAAGCAAAGATTTGAGGAAAAGAGGTTCAAATTGGACCACTCA
AATGGCCACAGGTGGCAGATATTTCAAGATTGGTTGGGAACTGACCAAGATAACCTTGATTTGGCCAATGTCAATCTTATGTTGGAGTTA
CTAGTGCAGAAGAAGAAACAACTGGAAGCAGAATCACATGCAGCCCAACTACAGATTCTTATGGAATTCCTCAAGGTTGCAAGAAGAAAT
AAGAGAGAGGAAATGAGTGGCTTATACTCTCCTGTCAGTGAGGATAGCACAGTGCCTCAATTTGAAGCTCCTTCTCCATCACACAGTAGT
ATTATTGATTCCACAGAATACAGCCAACCTCCAGGTTTCAGTGGCAGTTCTCAGACAAAGAAACAGCCTTGGTATAATAGCACGTTAGCA
TCAAGACGAAAACGACTTACTGCTCATTTTGAAGACTTGGAGCAGTGTTACTTTTCTACAAGGATGTCTCGTATCTCAGATGACAGTCGA
ACTGCAAGCCAGTTGGATGAATTTCAGGAATGCTTGTCCAAGTTTACTCGATATAATTCAGTACGACCTTTAGCCACATTGTCATATGCT
AGTGATCTCTATAATGGTTCCAGTATAGTCTCTAGTATTGAATTTGACCGGGATTGTGACTATTTTGCGATTGCTGGAGTTACAAAGAAG
ATTAAAGTCTATGAATATGACACTGTCATCCAGGATGCAGTGGATATTCATTACCCTGAGAATGAAATGACCTGCAATTCGAAAATCAGC
TGTATCAGTTGGAGTAGTTACCATAAGAACCTGTTAGCTAGCAGTGATTATGAAGGCACTGTTATTTTATGGGATGGATTCACAGGACAG
AGGTCAAAGGTCTATCAGGAGCATGAGAAGAGGTGTTGGAGTGTTGACTTTAATTTGATGGATCCTAAACTCTTGGCTTCAGGTTCTGAT
GATGCAAAAGTGAAGCTGTGGTCTACCAATCTAGACAACTCAGTGGCAAGCATTGAGGCAAAGGCTAATGTGTGCTGTGTTAAATTCAGC
CCCTCTTCCAGATACCATTTGGCTTTCGGCTGTGCAGATCACTGTGTCCACTACTATGATCTTCGTAACACTAAACAGCCAATCATGGTA
TTCAAAGGACACCGTAAAGCAGTCTCTTATGCAAAGTTTGTGAGTGGTGAGGAAATTGTCTCTGCCTCAACAGACAGTCAGCTAAAACTG
TGGAATGTAGGGAAACCATACTGCCTACGTTCCTTCAAGGGTCATATCAATGAAAAAAACTTTGTAGGCCTGGCTTCCAATGGAGATTAT
ATAGCTTGTGGAAGTGAAAATAACTCTCTCTACCTGTACTATAAAGGACTTTCTAAGACTTTGCTAACTTTTAAGTTTGATACAGTCAAA
AGTGTTCTCGACAAAGACCGAAAAGAAGATGATACAAATGAATTTGTTAGTGCTGTGTGCTGGAGGGCACTACCAGATGGGTTGGAGAAT
CTAATGCTGGACAAAGATGGCCACATAAAAATTACAGATTTTGGACTTTGCAAAGAAGGGATCACAGATGCAGCCACCATGAAGACATTC
TGTGGCACTCCAGAATATCTGGCACCAGAGGTGTTAGAAGATAATGACTATGGCCGAGCAGTAGACTGGTGGGGCCTAGGGGTTGTCATG
TATGAAATGATGTGTGGGAGGTTACCTTTCTACAACCAGGACCATGAGAAACTTTTTGAATTAATATTAATGGAAGACATTAAATTTCCT
CGAACACTCTCTTCAGATGCAAAATCATTGCTTTCAGGGCTCTTGATAAAGGATCCAAATAAACGCCTTGGTGGAGGACCAGATGATGCA
AAAGAAATTATGAGACACAGTTTCTTCTCTGGAGTAAACTGGCAAGATGTATATGATAAAAAGCTTGTACCTCCTTTTAAACCTCAAGTA
ACATCTGAGACAGATACTAGATATTTTGATGAAGAATTTACAGCTCAGACTATTACAATAACACCACCTGAAAAATGTCAGCAATCAGAT
TGTGGCATGCTGGGTAACTGGAAAAAATAATAAAAATCGGCTTCCTACAGCCAGCAGCACAGTCACCCATGGAACTGTTGGCTTTGGATT
AAATGTGGAATTGAACGACTACCCAGAAGTGTTCTGGAAAGAAGCGAGATGTGTGGCCTGCCTCACCGTCCTCACCCATCAAAAGCACCA

>73668_73668_2_RFWD2-AKT3_RFWD2_chr1_175956078_ENST00000308769_AKT3_chr1_243727150_ENST00000336199_length(amino acids)=879AA_BP=687
MSGSRQAGSGSAGTSPGSSAASSVTSASSSLSSSPSPPSVAVSAAALVSGGVAQAAGSGGLGGPVRPVLVAPAVSGSGGGAVSTGLSRHS
CAARPSAGVGGSSSSLGSGSRKRPLLAPLCNGLINSYEDKSNDFVCPICFDMIEEAYMTKCGHSFCYKCIHQSLEDNNRCPKCNYVVDNI
DHLYPNFLVNELILKQKQRFEEKRFKLDHSNGHRWQIFQDWLGTDQDNLDLANVNLMLELLVQKKKQLEAESHAAQLQILMEFLKVARRN
KREEMSGLYSPVSEDSTVPQFEAPSPSHSSIIDSTEYSQPPGFSGSSQTKKQPWYNSTLASRRKRLTAHFEDLEQCYFSTRMSRISDDSR
TASQLDEFQECLSKFTRYNSVRPLATLSYASDLYNGSSIVSSIEFDRDCDYFAIAGVTKKIKVYEYDTVIQDAVDIHYPENEMTCNSKIS
CISWSSYHKNLLASSDYEGTVILWDGFTGQRSKVYQEHEKRCWSVDFNLMDPKLLASGSDDAKVKLWSTNLDNSVASIEAKANVCCVKFS
PSSRYHLAFGCADHCVHYYDLRNTKQPIMVFKGHRKAVSYAKFVSGEEIVSASTDSQLKLWNVGKPYCLRSFKGHINEKNFVGLASNGDY
IACGSENNSLYLYYKGLSKTLLTFKFDTVKSVLDKDRKEDDTNEFVSAVCWRALPDGLENLMLDKDGHIKITDFGLCKEGITDAATMKTF
CGTPEYLAPEVLEDNDYGRAVDWWGLGVVMYEMMCGRLPFYNQDHEKLFELILMEDIKFPRTLSSDAKSLLSGLLIKDPNKRLGGGPDDA

--------------------------------------------------------------
>73668_73668_3_RFWD2-AKT3_RFWD2_chr1_175956078_ENST00000308769_AKT3_chr1_243727150_ENST00000366539_length(transcript)=6168nt_BP=2061nt
ATGTCTGGTAGCCGCCAGGCCGGGTCGGGCTCCGCTGGGACAAGCCCCGGGTCCTCGGCGGCCTCCTCGGTGACTTCCGCCTCCTCGTCT
TTATCCTCTTCCCCGTCGCCGCCTTCCGTGGCGGTTTCGGCGGCAGCGCTGGTGTCCGGCGGGGTGGCCCAGGCCGCCGGCTCGGGCGGC
CTCGGGGGCCCGGTGCGGCCTGTGTTGGTGGCGCCCGCCGTATCGGGTAGCGGCGGCGGGGCGGTGTCCACGGGCCTGTCCCGGCACAGC
TGCGCGGCCAGGCCCAGCGCCGGCGTAGGAGGCAGCAGCTCCAGCCTAGGCAGCGGCAGCAGGAAGCGACCTCTCCTCGCCCCCCTCTGC
AACGGGCTCATCAACTCCTACGAGGACAAAAGCAACGACTTCGTATGCCCCATCTGCTTTGATATGATTGAAGAAGCATACATGACAAAA
TGTGGCCACAGCTTTTGCTACAAGTGTATTCATCAGAGTTTGGAGGACAATAATAGATGTCCCAAGTGTAACTATGTTGTGGACAATATT
GACCATCTGTATCCTAATTTCTTGGTGAATGAACTCATTCTTAAACAGAAGCAAAGATTTGAGGAAAAGAGGTTCAAATTGGACCACTCA
AATGGCCACAGGTGGCAGATATTTCAAGATTGGTTGGGAACTGACCAAGATAACCTTGATTTGGCCAATGTCAATCTTATGTTGGAGTTA
CTAGTGCAGAAGAAGAAACAACTGGAAGCAGAATCACATGCAGCCCAACTACAGATTCTTATGGAATTCCTCAAGGTTGCAAGAAGAAAT
AAGAGAGAGGAAATGAGTGGCTTATACTCTCCTGTCAGTGAGGATAGCACAGTGCCTCAATTTGAAGCTCCTTCTCCATCACACAGTAGT
ATTATTGATTCCACAGAATACAGCCAACCTCCAGGTTTCAGTGGCAGTTCTCAGACAAAGAAACAGCCTTGGTATAATAGCACGTTAGCA
TCAAGACGAAAACGACTTACTGCTCATTTTGAAGACTTGGAGCAGTGTTACTTTTCTACAAGGATGTCTCGTATCTCAGATGACAGTCGA
ACTGCAAGCCAGTTGGATGAATTTCAGGAATGCTTGTCCAAGTTTACTCGATATAATTCAGTACGACCTTTAGCCACATTGTCATATGCT
AGTGATCTCTATAATGGTTCCAGTATAGTCTCTAGTATTGAATTTGACCGGGATTGTGACTATTTTGCGATTGCTGGAGTTACAAAGAAG
ATTAAAGTCTATGAATATGACACTGTCATCCAGGATGCAGTGGATATTCATTACCCTGAGAATGAAATGACCTGCAATTCGAAAATCAGC
TGTATCAGTTGGAGTAGTTACCATAAGAACCTGTTAGCTAGCAGTGATTATGAAGGCACTGTTATTTTATGGGATGGATTCACAGGACAG
AGGTCAAAGGTCTATCAGGAGCATGAGAAGAGGTGTTGGAGTGTTGACTTTAATTTGATGGATCCTAAACTCTTGGCTTCAGGTTCTGAT
GATGCAAAAGTGAAGCTGTGGTCTACCAATCTAGACAACTCAGTGGCAAGCATTGAGGCAAAGGCTAATGTGTGCTGTGTTAAATTCAGC
CCCTCTTCCAGATACCATTTGGCTTTCGGCTGTGCAGATCACTGTGTCCACTACTATGATCTTCGTAACACTAAACAGCCAATCATGGTA
TTCAAAGGACACCGTAAAGCAGTCTCTTATGCAAAGTTTGTGAGTGGTGAGGAAATTGTCTCTGCCTCAACAGACAGTCAGCTAAAACTG
TGGAATGTAGGGAAACCATACTGCCTACGTTCCTTCAAGGGTCATATCAATGAAAAAAACTTTGTAGGCCTGGCTTCCAATGGAGATTAT
ATAGCTTGTGGAAGTGAAAATAACTCTCTCTACCTGTACTATAAAGGACTTTCTAAGACTTTGCTAACTTTTAAGTTTGATACAGTCAAA
AGTGTTCTCGACAAAGACCGAAAAGAAGATGATACAAATGAATTTGTTAGTGCTGTGTGCTGGAGGGCACTACCAGATGGGTTGGAGAAT
CTAATGCTGGACAAAGATGGCCACATAAAAATTACAGATTTTGGACTTTGCAAAGAAGGGATCACAGATGCAGCCACCATGAAGACATTC
TGTGGCACTCCAGAATATCTGGCACCAGAGGTGTTAGAAGATAATGACTATGGCCGAGCAGTAGACTGGTGGGGCCTAGGGGTTGTCATG
TATGAAATGATGTGTGGGAGGTTACCTTTCTACAACCAGGACCATGAGAAACTTTTTGAATTAATATTAATGGAAGACATTAAATTTCCT
CGAACACTCTCTTCAGATGCAAAATCATTGCTTTCAGGGCTCTTGATAAAGGATCCAAATAAACGCCTTGGTGGAGGACCAGATGATGCA
AAAGAAATTATGAGACACAGTTTCTTCTCTGGAGTAAACTGGCAAGATGTATATGATAAAAAGCTTGTACCTCCTTTTAAACCTCAAGTA
ACATCTGAGACAGATACTAGATATTTTGATGAAGAATTTACAGCTCAGACTATTACAATAACACCACCTGAAAAATATGATGAGGATGGT
ATGGACTGCATGGACAATGAGAGGCGGCCGCATTTCCCTCAATTTTCCTACTCTGCAAGTGGACGAGAATAAGTCTCTTTCATTCTGCTA
CTTCACTGTCATCTTCAATTTATTACTGAAAATGATTCCTGGACATCACCAGTCCTAGCTCTTACACATAGCAGGGGCACCTTCCGACAT
CCCAGACCAGCCAAGGGTCCTCACCCCTCGCCACCTTTCACCCTCATGAAAACACACATACACGCAAATACACTCCAGTTTTTGTTTTTG
CATGAAATTGTATCTCAGTCTAAGGTCTCATGCTGTTGCTGCTACTGTCTTACTATTATAGCAACTTTAAGAAGTAATTTTCCAACCTTT
GGAAGTCATGAGCCCACCATTGTTCATTTGTGCACCAATTATCATCTTTTGATCTTTTAGTTTTTCCCTCAGTGAAGGCTAAATGAGATA
CACTGATTCTAGGTACATTTTTTAACTTTCTAGAAGAGAAAAACTAACTAGACTAAGAAGATTTAGTTTATAAATTCAGAACAAGCAATT
GTGGAAGGGTGGTGGCGTGCATATGTAAAGCACATCAGATCCGTGCGTGAAGTAGGCATATATCACTAAGCTGTGGCTGGAATTGATTAG
GAAGCATTTGGTAGAAGGACTGAACAACTGTTGGGATATATATATATATATATAATTTTTTTTTTTTAAATTCCTGGTGGATACTGTAGA
AGAAGCCCATATCACATGTGGATGTCGAGACTTCACGGGCAATCATGAGCAAGTGAACACTGTTCTACCAAGAACTGAAGGCATATGCAC
AGTCAAGGTCACTTAAAGGGTCTTATGAAACAATTTGAGCCAGAGAGCATCTTTCCCCTGTGCTTGGAAACCTTTTTTCCTTCTTGACAT
TTATCACCTCTGATGGCTGAAGAATGTAGACAGGTATAATGATACTGCTTTTCACCAAAATTTCTACACCAAGGTAAACAGGTGTTTGCC
TTATTTAATTTTTTACTTTCAGTTCTACGTGAATTAGCTTTTTCTCAGATGTTGAAACTTTGAATGTCCTTTTATGATTTTGTTTATATT
GCAGTAGTATTTATTTTTTAGTGATGAGAATTGTATGTCATGTTAGCAAACGCAGCTCCAACTTATATAAAATAGACTTACTGCAGTTAC
TTTTGACCCATGTGCAAGGATTGTACACGCTGATGAGAATCATGCACTTTTTCTCCTCTGTTAAAAAAAATGATAAGGCTCTGAAATGGA
ATATATTGGTTAGAATTTGGCTTTGGGAGAAGAGATGCTGCCATTTAACCCCTTGGTACTGAAAATGAGAAAATCCCCAACTATGCATGC
CAAGGGGTTAATGAAACAAATAGCTGTTGACGTTTGCTCATTTAAGAATTTGAAACGTTATGATGACCTGGCAACAAAAAGTAATGAAGA
AAATTGAGACCTGAGTGAAGATAAGAAATGATCTTTACGTGGCAAAATGAACACATCTTGAGTATTTAGGAAATGGGCAGTGAAGGCTAA
GAACCTGGTGTGTTTCTTGGGATCATGGTACATTTATCACTGAATTAAGCCATCAGGGAAAAAACAACAAAAAAAGAGAACACCTCCAGC
TTTTCTTTTTCTGTATATACTCATGTCCCCCAGATTCCAACATTTCTCACTGAAAGGGGGCATGTATGCAAACCTCATCTTTCTCCTTCA
TTAATGATGATCTTCAGATTAAACCCTTTGGTGCTAGGAGCTGACAATTTCCAAAGCAGCCTGTGAAGTCCTAGGGGCTGGGGGCCACTC
TTGCGGCAAGCAGAAGGCCATCCTACTCCGCGGAGTGATCATGGAAATGTATTTTAGTTAAACTCTGACAGCTCCCAAACGGAAGACTAC
AGCATGACGTAGTATTATGATTGCATTGTATGAAAGAGCAAGTGACTTTCTAAGTAGGATGAATCATATTCATATGCAGATGTCTTAGCC
TCTTGACGCTGGAAGTGTGGATTTATAGCTATGAAACCACTGCTGGCAGTGGGTGGGCCACTGGGACTGACGGGGGTTAAAGGGCATTTT
ACTAAGGCAGCTAAGACATATTCAGACATCAACGTTATCCTTCTTTTTCATATTTCTACCTGAGTGAAGTTCATCCTTAGTATTGAGTAG
GAAGTTACAGTAAATGGTAGTTCATTCTTACTTACACACATAGCTAATCTTTTTTTTTTCACTTGGAATTATGTTGAATGTTTCATTTTG
ACAAAAAAGTAGACTAGAAGGTATGTTCTTTAAGTTGTCTTGCATCCATTATATAAGAAAGAAACAGGTGAGAGGAAGAGCAGAAAGCTG
AGACTGGCTGATGTTCAGAGCACTTACTCCTCTAGAGGGAAAGCATGACACCGAACACTAAGCACACAGCTTTTTGTTGTTTTGGTTTTT
TCTCCCGCAAATCTTAAAGTGATTCCCATGACCTTGGCCAAGGACACTTCTTAAAGATTAATGACTGGCACTGACATTGCCCCAGGCGGG
CCACTCCTCACACTGGCTCTCAGTTCCCAGCCATGCCTGGGGCTCAGTCACTTCTATTCCACCCTCTGAGACTCCATTGGTGTCACACAA
GGTGTCTTCTTGGCTTTGATTTTGAGAATCCCCTATTTTCACTTCCAGATCTGTCAGCTGCCATGGAGGAATAATAGAAAACCAGAAATG
CGTGTAGAGGGAGATTTCTAAAACTTCCCTTGTGTCGCCCATAGTTGTAGTTTTGGGTTCTGGCAGGTGGAACACCCTGAAACCTGGAAT
CATTCTATGAGAATACAGTTCAGACTTTGCAGACTCCAGCCCATACTAACTGTCATGAAGCTTGACTTCTTGTCATAATGCAGCCATCTT
GGAGGAAATTGGCCATTTCTGCTTAGATGGTTGGCAGGGTCGCGCTCAGCTTTGCTTTCTACACTAATTACATAGCATTATTCAAGTATT
GTTTTCCATTTCCCATCCCTGATTTCCAGCTTCTTAAAGCTGACTGTTCTTGCAGGGGCCACTTGCTTCTCCTAGAGTACAAAAGTAAGG
GCCTTCCTTACTAACTGCAGGGTCTCTCTATTACACCTCAACATACACACTTTGCTGCTACTGTTTGTACTGTCTACAGTAGAATTTCCT
TATCTTGCTCCTGGTAGTGCATTACAGGCAAGCATGAAATGTAAAGTATTTATTTAAATAAAAAGAAAACCTCTAAATTGGTAATTGAAT
TACCTCCCTGTAGCTTTATAGTTTGTGACATTTCTTGACCTTGCTAGTTCTTTCATTAGATCTGCGCAAGATCTAGTCATCTGGTTAAGG
ATTTTAAGCAGATGCAACTATAAACCCAAGAAACTGTATTACTATTACTGTTGGTCATACTAAACCTGTCTATTTCCTGAAGTATATGAC
CCACAAGGATGTGGAATAACTAGGAGAAACTGTTTTTGTACACTGTACATCCTTAGTATTTTTACACGTATATGATAGGGATGAACATGA

>73668_73668_3_RFWD2-AKT3_RFWD2_chr1_175956078_ENST00000308769_AKT3_chr1_243727150_ENST00000366539_length(amino acids)=893AA_BP=687
MSGSRQAGSGSAGTSPGSSAASSVTSASSSLSSSPSPPSVAVSAAALVSGGVAQAAGSGGLGGPVRPVLVAPAVSGSGGGAVSTGLSRHS
CAARPSAGVGGSSSSLGSGSRKRPLLAPLCNGLINSYEDKSNDFVCPICFDMIEEAYMTKCGHSFCYKCIHQSLEDNNRCPKCNYVVDNI
DHLYPNFLVNELILKQKQRFEEKRFKLDHSNGHRWQIFQDWLGTDQDNLDLANVNLMLELLVQKKKQLEAESHAAQLQILMEFLKVARRN
KREEMSGLYSPVSEDSTVPQFEAPSPSHSSIIDSTEYSQPPGFSGSSQTKKQPWYNSTLASRRKRLTAHFEDLEQCYFSTRMSRISDDSR
TASQLDEFQECLSKFTRYNSVRPLATLSYASDLYNGSSIVSSIEFDRDCDYFAIAGVTKKIKVYEYDTVIQDAVDIHYPENEMTCNSKIS
CISWSSYHKNLLASSDYEGTVILWDGFTGQRSKVYQEHEKRCWSVDFNLMDPKLLASGSDDAKVKLWSTNLDNSVASIEAKANVCCVKFS
PSSRYHLAFGCADHCVHYYDLRNTKQPIMVFKGHRKAVSYAKFVSGEEIVSASTDSQLKLWNVGKPYCLRSFKGHINEKNFVGLASNGDY
IACGSENNSLYLYYKGLSKTLLTFKFDTVKSVLDKDRKEDDTNEFVSAVCWRALPDGLENLMLDKDGHIKITDFGLCKEGITDAATMKTF
CGTPEYLAPEVLEDNDYGRAVDWWGLGVVMYEMMCGRLPFYNQDHEKLFELILMEDIKFPRTLSSDAKSLLSGLLIKDPNKRLGGGPDDA

--------------------------------------------------------------
>73668_73668_4_RFWD2-AKT3_RFWD2_chr1_175956078_ENST00000308769_AKT3_chr1_243727150_ENST00000366540_length(transcript)=2664nt_BP=2061nt
ATGTCTGGTAGCCGCCAGGCCGGGTCGGGCTCCGCTGGGACAAGCCCCGGGTCCTCGGCGGCCTCCTCGGTGACTTCCGCCTCCTCGTCT
TTATCCTCTTCCCCGTCGCCGCCTTCCGTGGCGGTTTCGGCGGCAGCGCTGGTGTCCGGCGGGGTGGCCCAGGCCGCCGGCTCGGGCGGC
CTCGGGGGCCCGGTGCGGCCTGTGTTGGTGGCGCCCGCCGTATCGGGTAGCGGCGGCGGGGCGGTGTCCACGGGCCTGTCCCGGCACAGC
TGCGCGGCCAGGCCCAGCGCCGGCGTAGGAGGCAGCAGCTCCAGCCTAGGCAGCGGCAGCAGGAAGCGACCTCTCCTCGCCCCCCTCTGC
AACGGGCTCATCAACTCCTACGAGGACAAAAGCAACGACTTCGTATGCCCCATCTGCTTTGATATGATTGAAGAAGCATACATGACAAAA
TGTGGCCACAGCTTTTGCTACAAGTGTATTCATCAGAGTTTGGAGGACAATAATAGATGTCCCAAGTGTAACTATGTTGTGGACAATATT
GACCATCTGTATCCTAATTTCTTGGTGAATGAACTCATTCTTAAACAGAAGCAAAGATTTGAGGAAAAGAGGTTCAAATTGGACCACTCA
AATGGCCACAGGTGGCAGATATTTCAAGATTGGTTGGGAACTGACCAAGATAACCTTGATTTGGCCAATGTCAATCTTATGTTGGAGTTA
CTAGTGCAGAAGAAGAAACAACTGGAAGCAGAATCACATGCAGCCCAACTACAGATTCTTATGGAATTCCTCAAGGTTGCAAGAAGAAAT
AAGAGAGAGGAAATGAGTGGCTTATACTCTCCTGTCAGTGAGGATAGCACAGTGCCTCAATTTGAAGCTCCTTCTCCATCACACAGTAGT
ATTATTGATTCCACAGAATACAGCCAACCTCCAGGTTTCAGTGGCAGTTCTCAGACAAAGAAACAGCCTTGGTATAATAGCACGTTAGCA
TCAAGACGAAAACGACTTACTGCTCATTTTGAAGACTTGGAGCAGTGTTACTTTTCTACAAGGATGTCTCGTATCTCAGATGACAGTCGA
ACTGCAAGCCAGTTGGATGAATTTCAGGAATGCTTGTCCAAGTTTACTCGATATAATTCAGTACGACCTTTAGCCACATTGTCATATGCT
AGTGATCTCTATAATGGTTCCAGTATAGTCTCTAGTATTGAATTTGACCGGGATTGTGACTATTTTGCGATTGCTGGAGTTACAAAGAAG
ATTAAAGTCTATGAATATGACACTGTCATCCAGGATGCAGTGGATATTCATTACCCTGAGAATGAAATGACCTGCAATTCGAAAATCAGC
TGTATCAGTTGGAGTAGTTACCATAAGAACCTGTTAGCTAGCAGTGATTATGAAGGCACTGTTATTTTATGGGATGGATTCACAGGACAG
AGGTCAAAGGTCTATCAGGAGCATGAGAAGAGGTGTTGGAGTGTTGACTTTAATTTGATGGATCCTAAACTCTTGGCTTCAGGTTCTGAT
GATGCAAAAGTGAAGCTGTGGTCTACCAATCTAGACAACTCAGTGGCAAGCATTGAGGCAAAGGCTAATGTGTGCTGTGTTAAATTCAGC
CCCTCTTCCAGATACCATTTGGCTTTCGGCTGTGCAGATCACTGTGTCCACTACTATGATCTTCGTAACACTAAACAGCCAATCATGGTA
TTCAAAGGACACCGTAAAGCAGTCTCTTATGCAAAGTTTGTGAGTGGTGAGGAAATTGTCTCTGCCTCAACAGACAGTCAGCTAAAACTG
TGGAATGTAGGGAAACCATACTGCCTACGTTCCTTCAAGGGTCATATCAATGAAAAAAACTTTGTAGGCCTGGCTTCCAATGGAGATTAT
ATAGCTTGTGGAAGTGAAAATAACTCTCTCTACCTGTACTATAAAGGACTTTCTAAGACTTTGCTAACTTTTAAGTTTGATACAGTCAAA
AGTGTTCTCGACAAAGACCGAAAAGAAGATGATACAAATGAATTTGTTAGTGCTGTGTGCTGGAGGGCACTACCAGATGGGTTGGAGAAT
CTAATGCTGGACAAAGATGGCCACATAAAAATTACAGATTTTGGACTTTGCAAAGAAGGGATCACAGATGCAGCCACCATGAAGACATTC
TGTGGCACTCCAGAATATCTGGCACCAGAGGTGTTAGAAGATAATGACTATGGCCGAGCAGTAGACTGGTGGGGCCTAGGGGTTGTCATG
TATGAAATGATGTGTGGGAGGTTACCTTTCTACAACCAGGACCATGAGAAACTTTTTGAATTAATATTAATGGAAGACATTAAATTTCCT
CGAACACTCTCTTCAGATGCAAAATCATTGCTTTCAGGGCTCTTGATAAAGGATCCAAATAAACGCCTTGGTGGAGGACCAGATGATGCA
AAAGAAATTATGAGACACAGTTTCTTCTCTGGAGTAAACTGGCAAGATGTATATGATAAAAAGCTTGTACCTCCTTTTAAACCTCAAGTA
ACATCTGAGACAGATACTAGATATTTTGATGAAGAATTTACAGCTCAGACTATTACAATAACACCACCTGAAAAATGTCAGCAATCAGAT

>73668_73668_4_RFWD2-AKT3_RFWD2_chr1_175956078_ENST00000308769_AKT3_chr1_243727150_ENST00000366540_length(amino acids)=879AA_BP=687
MSGSRQAGSGSAGTSPGSSAASSVTSASSSLSSSPSPPSVAVSAAALVSGGVAQAAGSGGLGGPVRPVLVAPAVSGSGGGAVSTGLSRHS
CAARPSAGVGGSSSSLGSGSRKRPLLAPLCNGLINSYEDKSNDFVCPICFDMIEEAYMTKCGHSFCYKCIHQSLEDNNRCPKCNYVVDNI
DHLYPNFLVNELILKQKQRFEEKRFKLDHSNGHRWQIFQDWLGTDQDNLDLANVNLMLELLVQKKKQLEAESHAAQLQILMEFLKVARRN
KREEMSGLYSPVSEDSTVPQFEAPSPSHSSIIDSTEYSQPPGFSGSSQTKKQPWYNSTLASRRKRLTAHFEDLEQCYFSTRMSRISDDSR
TASQLDEFQECLSKFTRYNSVRPLATLSYASDLYNGSSIVSSIEFDRDCDYFAIAGVTKKIKVYEYDTVIQDAVDIHYPENEMTCNSKIS
CISWSSYHKNLLASSDYEGTVILWDGFTGQRSKVYQEHEKRCWSVDFNLMDPKLLASGSDDAKVKLWSTNLDNSVASIEAKANVCCVKFS
PSSRYHLAFGCADHCVHYYDLRNTKQPIMVFKGHRKAVSYAKFVSGEEIVSASTDSQLKLWNVGKPYCLRSFKGHINEKNFVGLASNGDY
IACGSENNSLYLYYKGLSKTLLTFKFDTVKSVLDKDRKEDDTNEFVSAVCWRALPDGLENLMLDKDGHIKITDFGLCKEGITDAATMKTF
CGTPEYLAPEVLEDNDYGRAVDWWGLGVVMYEMMCGRLPFYNQDHEKLFELILMEDIKFPRTLSSDAKSLLSGLLIKDPNKRLGGGPDDA

--------------------------------------------------------------
>73668_73668_5_RFWD2-AKT3_RFWD2_chr1_175956078_ENST00000367669_AKT3_chr1_243727150_ENST00000263826_length(transcript)=5336nt_BP=2648nt
CACCTATACCATTCATTCCAGTCTCCTCAATTCTTTCACTGTATTAAGACGGCCTGCCCTCTGGCACGTTTTCTGGAACGAAAGCGGCTT
GGGACAGACAGACTCCCTTCGCCAGGGAGCCCCGACCCCGAGGGCGCGCCCTGAGCGTCTGAGCGCAGCCCCGCTGCGTCCCCAGTTGTA
TCCCCCGCCACGCCCGCCTCTTCCGCCGCTCGCGCGCGCGCGCACGGCCGGCCACGGCGCGCCGGGGTTGGCTGCTACAGGGACCGCGGT
GGCGGCGGCGGCCTCGACAGCGGTAGTGCCTTCTACTCCGCTTTTTTAGTTTCCTCCGCCCCCTCCCGTGGGACGCTGTTGTGTGGCTGC
CTTAAAAAAAAACGCAACTTTATTGTTCCTCAGCCCCACCTCCGGCTCGGCGGGCGTCCTCAGGATGCACTGAGGCTGAGGGGAGGGGAG
GCGGCGGAGGGTCGAGGTCGCGGTCCCTCTCCTCCGAGCGCCCGGCTGGAGGGGAGGGAGTCACGATGTCTGGTAGCCGCCAGGCCGGGT
CGGGCTCCGCTGGGACAAGCCCCGGGTCCTCGGCGGCCTCCTCGGTGACTTCCGCCTCCTCGTCTTTATCCTCTTCCCCGTCGCCGCCTT
CCGTGGCGGTTTCGGCGGCAGCGCTGGTGTCCGGCGGGGTGGCCCAGGCCGCCGGCTCGGGCGGCCTCGGGGGCCCGGTGCGGCCTGTGT
TGGTGGCGCCCGCCGTATCGGGTAGCGGCGGCGGGGCGGTGTCCACGGGCCTGTCCCGGCACAGCTGCGCGGCCAGGCCCAGCGCCGGCG
TAGGAGGCAGCAGCTCCAGCCTAGGCAGCGGCAGCAGGAAGCGACCTCTCCTCGCCCCCCTCTGCAACGGGCTCATCAACTCCTACGAGG
ACAAAAGCAACGACTTCGTATGCCCCATCTGCTTTGATATGATTGAAGAAGCATACATGACAAAATGTGGCCACAGCTTTTGCTACAAGT
GTATTCATCAGAGTTTGGAGGACAATAATAGATGTCCCAAGTGTAACTATGTTGTGGACAATATTGACCATCTGTATCCTAATTTCTTGG
TGAATGAACTCATTCTTAAACAGAAGCAAAGATTTGAGGAAAAGAGGTTCAAATTGGACCACTCAGTGAGTAGCACCAATGGCCACAGGT
GGCAGATATTTCAAGATTGGTTGGGAACTGACCAAGATAACCTTGATTTGGCCAATGTCAATCTTATGTTGGAGTTACTAGTGCAGAAGA
AGAAACAACTGGAAGCAGAATCACATGCAGCCCAACTACAGATTCTTATGGAATTCCTCAAGGTTGCAAGAAGAAATAAGAGAGAGCAAC
TGGAACAGATCCAGAAGGAGCTAAGTGTTTTGGAAGAGGATATTAAGAGAGTGGAAGAAATGAGTGGCTTATACTCTCCTGTCAGTGAGG
ATAGCACAGTGCCTCAATTTGAAGCTCCTTCTCCATCACACAGTAGTATTATTGATTCCACAGAATACAGCCAACCTCCAGGTTTCAGTG
GCAGTTCTCAGACAAAGAAACAGCCTTGGTATAATAGCACGTTAGCATCAAGACGAAAACGACTTACTGCTCATTTTGAAGACTTGGAGC
AGTGTTACTTTTCTACAAGGATGTCTCGTATCTCAGATGACAGTCGAACTGCAAGCCAGTTGGATGAATTTCAGGAATGCTTGTCCAAGT
TTACTCGATATAATTCAGTACGACCTTTAGCCACATTGTCATATGCTAGTGATCTCTATAATGGTTCCAGTATAGTCTCTAGTATTGAAT
TTGACCGGGATTGTGACTATTTTGCGATTGCTGGAGTTACAAAGAAGATTAAAGTCTATGAATATGACACTGTCATCCAGGATGCAGTGG
ATATTCATTACCCTGAGAATGAAATGACCTGCAATTCGAAAATCAGCTGTATCAGTTGGAGTAGTTACCATAAGAACCTGTTAGCTAGCA
GTGATTATGAAGGCACTGTTATTTTATGGGATGGATTCACAGGACAGAGGTCAAAGGTCTATCAGGAGCATGAGAAGAGGTGTTGGAGTG
TTGACTTTAATTTGATGGATCCTAAACTCTTGGCTTCAGGTTCTGATGATGCAAAAGTGAAGCTGTGGTCTACCAATCTAGACAACTCAG
TGGCAAGCATTGAGGCAAAGGCTAATGTGTGCTGTGTTAAATTCAGCCCCTCTTCCAGATACCATTTGGCTTTCGGCTGTGCAGATCACT
GTGTCCACTACTATGATCTTCGTAACACTAAACAGCCAATCATGGTATTCAAAGGACACCGTAAAGCAGTCTCTTATGCAAAGTTTGTGA
GTGGTGAGGAAATTGTCTCTGCCTCAACAGACAGTCAGCTAAAACTGTGGAATGTAGGGAAACCATACTGCCTACGTTCCTTCAAGGGTC
ATATCAATGAAAAAAACTTTGTAGGCCTGGCTTCCAATGGAGATTATATAGCTTGTGGAAGTGAAAATAACTCTCTCTACCTGTACTATA
AAGGACTTTCTAAGACTTTGCTAACTTTTAAGTTTGATACAGTCAAAAGTGTTCTCGACAAAGACCGAAAAGAAGATGATACAAATGAAT
TTGTTAGTGCTGTGTGCTGGAGGGCACTACCAGATGGGTTGGAGAATCTAATGCTGGACAAAGATGGCCACATAAAAATTACAGATTTTG
GACTTTGCAAAGAAGGGATCACAGATGCAGCCACCATGAAGACATTCTGTGGCACTCCAGAATATCTGGCACCAGAGGTGTTAGAAGATA
ATGACTATGGCCGAGCAGTAGACTGGTGGGGCCTAGGGGTTGTCATGTATGAAATGATGTGTGGGAGGTTACCTTTCTACAACCAGGACC
ATGAGAAACTTTTTGAATTAATATTAATGGAAGACATTAAATTTCCTCGAACACTCTCTTCAGATGCAAAATCATTGCTTTCAGGGCTCT
TGATAAAGGATCCAAATAAACGCCTTGGTGGAGGACCAGATGATGCAAAAGAAATTATGAGACACAGTTTCTTCTCTGGAGTAAACTGGC
AAGATGTATATGATAAAAAGCTTGTACCTCCTTTTAAACCTCAAGTAACATCTGAGACAGATACTAGATATTTTGATGAAGAATTTACAG
CTCAGACTATTACAATAACACCACCTGAAAAATATGATGAGGATGGTATGGACTGCATGGACAATGAGAGGCGGCCGCATTTCCCTCAAT
TTTCCTACTCTGCAAGTGGACGAGAATAAGTCTCTTTCATTCTGCTACTTCACTGTCATCTTCAATTTATTACTGAAAATGATTCCTGGA
CATCACCAGTCCTAGCTCTTACACATAGCAGGGGCACCTTCCGACATCCCAGACCAGCCAAGGGTCCTCACCCCTCGCCACCTTTCACCC
TCATGAAAACACACATACACGCAAATACACTCCAGTTTTTGTTTTTGCATGAAATTGTATCTCAGTCTAAGGTCTCATGCTGTTGCTGCT
ACTGTCTTACTATTATAGCAACTTTAAGAAGTAATTTTCCAACCTTTGGAAGTCATGAGCCCACCATTGTTCATTTGTGCACCAATTATC
ATCTTTTGATCTTTTAGTTTTTCCCTCAGTGAAGGCTAAATGAGATACACTGATTCTAGGTACATTTTTTAACTTTCTAGAAGAGAAAAA
CTAACTAGACTAAGAAGATTTAGTTTATAAATTCAGAACAAGCAATTGTGGAAGGGTGGTGGCGTGCATATGTAAAGCACATCAGATCCG
TGCGTGAAGTAGGCATATATCACTAAGCTGTGGCTGGAATTGATTAGGAAGCATTTGGTAGAAGGACTGAACAACTGTTGGGATATATAT
ATATATATATAATTTTTTTTTTTTAAATTCCTGGTGGATACTGTAGAAGAAGCCCATATCACATGTGGATGTCGAGACTTCACGGGCAAT
CATGAGCAAGTGAACACTGTTCTACCAAGAACTGAAGGCATATGCACAGTCAAGGTCACTTAAAGGGTCTTATGAAACAATTTGAGCCAG
AGAGCATCTTTCCCCTGTGCTTGGAAACCTTTTTTCCTTCTTGACATTTATCACCTCTGATGGCTGAAGAATGTAGACAGGTATAATGAT
ACTGCTTTTCACCAAAATTTCTACACCAAGGTAAACAGGTGTTTGCCTTATTTAATTTTTTACTTTCAGTTCTACGTGAATTAGCTTTTT
CTCAGATGTTGAAACTTTGAATGTCCTTTTATGATTTTGTTTATATTGCAGTAGTATTTATTTTTTAGTGATGAGAATTGTATGTCATGT
TAGCAAACGCAGCTCCAACTTATATAAAATAGACTTACTGCAGTTACTTTTGACCCATGTGCAAGGATTGTACACGCTGATGAGAATCAT
GCACTTTTTCTCCTCTGTTAAAAAAAATGATAAGGCTCTGAAATGGAATATATTGGTTAGAATTTGGCTTTGGGAGAAGAGATGCTGCCA
TTTAACCCCTTGGTACTGAAAATGAGAAAATCCCCAACTATGCATGCCAAGGGGTTAATGAAACAAATAGCTGTTGACGTTTGCTCATTT
AAGAATTTGAAACGTTATGATGACCTGGCAACAAAAAGTAATGAAGAAAATTGAGACCTGAGTGAAGATAAGAAATGATCTTTACGTGGC
AAAATGAACACATCTTGAGTATTTAGGAAATGGGCAGTGAAGGCTAAGAACCTGGTGTGTTTCTTGGGATCATGGTACATTTATCACTGA
ATTAAGCCATCAGGGAAAAAACAACAAAAAAAGAGAACACCTCCAGCTTTTCTTTTTCTGTATATACTCATGTCCCCCAGATTCCAACAT
TTCTCACTGAAAGGGGGCATGTATGCAAACCTCATCTTTCTCCTTCATTAATGATGATCTTCAGATTAAACCCTTTGGTGCTAGGAGCTG
ACAATTTCCAAAGCAGCCTGTGAAGTCCTAGGGGCTGGGGGCCACTCTTGCGGCAAGCAGAAGGCCATCCTACTCCGCGGAGTGATCATG
GAAATGTATTTTAGTTAAACTCTGACAGCTCCCAAACGGAAGACTACAGCATGACGTAGTATTATGATTGCATTGTATGAAAGAGCAAGT
GACTTTCTAAGTAGGATGAATCATATTCATATGCAGATGTCTTAGCCTCTTGACGCTGGAAGTGTGGATTTATAGCTATGAAACCACTGC
TGGCAGTGGGTGGGCCACTGGGACTGACGGGGGTTAAAGGGCATTTTACTAAGGCAGCTAAGACATATTCAGACATCAACGTTATCCTTC

>73668_73668_5_RFWD2-AKT3_RFWD2_chr1_175956078_ENST00000367669_AKT3_chr1_243727150_ENST00000263826_length(amino acids)=917AA_BP=711
MSGSRQAGSGSAGTSPGSSAASSVTSASSSLSSSPSPPSVAVSAAALVSGGVAQAAGSGGLGGPVRPVLVAPAVSGSGGGAVSTGLSRHS
CAARPSAGVGGSSSSLGSGSRKRPLLAPLCNGLINSYEDKSNDFVCPICFDMIEEAYMTKCGHSFCYKCIHQSLEDNNRCPKCNYVVDNI
DHLYPNFLVNELILKQKQRFEEKRFKLDHSVSSTNGHRWQIFQDWLGTDQDNLDLANVNLMLELLVQKKKQLEAESHAAQLQILMEFLKV
ARRNKREQLEQIQKELSVLEEDIKRVEEMSGLYSPVSEDSTVPQFEAPSPSHSSIIDSTEYSQPPGFSGSSQTKKQPWYNSTLASRRKRL
TAHFEDLEQCYFSTRMSRISDDSRTASQLDEFQECLSKFTRYNSVRPLATLSYASDLYNGSSIVSSIEFDRDCDYFAIAGVTKKIKVYEY
DTVIQDAVDIHYPENEMTCNSKISCISWSSYHKNLLASSDYEGTVILWDGFTGQRSKVYQEHEKRCWSVDFNLMDPKLLASGSDDAKVKL
WSTNLDNSVASIEAKANVCCVKFSPSSRYHLAFGCADHCVHYYDLRNTKQPIMVFKGHRKAVSYAKFVSGEEIVSASTDSQLKLWNVGKP
YCLRSFKGHINEKNFVGLASNGDYIACGSENNSLYLYYKGLSKTLLTFKFDTVKSVLDKDRKEDDTNEFVSAVCWRALPDGLENLMLDKD
GHIKITDFGLCKEGITDAATMKTFCGTPEYLAPEVLEDNDYGRAVDWWGLGVVMYEMMCGRLPFYNQDHEKLFELILMEDIKFPRTLSSD
AKSLLSGLLIKDPNKRLGGGPDDAKEIMRHSFFSGVNWQDVYDKKLVPPFKPQVTSETDTRYFDEEFTAQTITITPPEKYDEDGMDCMDN

--------------------------------------------------------------
>73668_73668_6_RFWD2-AKT3_RFWD2_chr1_175956078_ENST00000367669_AKT3_chr1_243727150_ENST00000336199_length(transcript)=3451nt_BP=2648nt
CACCTATACCATTCATTCCAGTCTCCTCAATTCTTTCACTGTATTAAGACGGCCTGCCCTCTGGCACGTTTTCTGGAACGAAAGCGGCTT
GGGACAGACAGACTCCCTTCGCCAGGGAGCCCCGACCCCGAGGGCGCGCCCTGAGCGTCTGAGCGCAGCCCCGCTGCGTCCCCAGTTGTA
TCCCCCGCCACGCCCGCCTCTTCCGCCGCTCGCGCGCGCGCGCACGGCCGGCCACGGCGCGCCGGGGTTGGCTGCTACAGGGACCGCGGT
GGCGGCGGCGGCCTCGACAGCGGTAGTGCCTTCTACTCCGCTTTTTTAGTTTCCTCCGCCCCCTCCCGTGGGACGCTGTTGTGTGGCTGC
CTTAAAAAAAAACGCAACTTTATTGTTCCTCAGCCCCACCTCCGGCTCGGCGGGCGTCCTCAGGATGCACTGAGGCTGAGGGGAGGGGAG
GCGGCGGAGGGTCGAGGTCGCGGTCCCTCTCCTCCGAGCGCCCGGCTGGAGGGGAGGGAGTCACGATGTCTGGTAGCCGCCAGGCCGGGT
CGGGCTCCGCTGGGACAAGCCCCGGGTCCTCGGCGGCCTCCTCGGTGACTTCCGCCTCCTCGTCTTTATCCTCTTCCCCGTCGCCGCCTT
CCGTGGCGGTTTCGGCGGCAGCGCTGGTGTCCGGCGGGGTGGCCCAGGCCGCCGGCTCGGGCGGCCTCGGGGGCCCGGTGCGGCCTGTGT
TGGTGGCGCCCGCCGTATCGGGTAGCGGCGGCGGGGCGGTGTCCACGGGCCTGTCCCGGCACAGCTGCGCGGCCAGGCCCAGCGCCGGCG
TAGGAGGCAGCAGCTCCAGCCTAGGCAGCGGCAGCAGGAAGCGACCTCTCCTCGCCCCCCTCTGCAACGGGCTCATCAACTCCTACGAGG
ACAAAAGCAACGACTTCGTATGCCCCATCTGCTTTGATATGATTGAAGAAGCATACATGACAAAATGTGGCCACAGCTTTTGCTACAAGT
GTATTCATCAGAGTTTGGAGGACAATAATAGATGTCCCAAGTGTAACTATGTTGTGGACAATATTGACCATCTGTATCCTAATTTCTTGG
TGAATGAACTCATTCTTAAACAGAAGCAAAGATTTGAGGAAAAGAGGTTCAAATTGGACCACTCAGTGAGTAGCACCAATGGCCACAGGT
GGCAGATATTTCAAGATTGGTTGGGAACTGACCAAGATAACCTTGATTTGGCCAATGTCAATCTTATGTTGGAGTTACTAGTGCAGAAGA
AGAAACAACTGGAAGCAGAATCACATGCAGCCCAACTACAGATTCTTATGGAATTCCTCAAGGTTGCAAGAAGAAATAAGAGAGAGCAAC
TGGAACAGATCCAGAAGGAGCTAAGTGTTTTGGAAGAGGATATTAAGAGAGTGGAAGAAATGAGTGGCTTATACTCTCCTGTCAGTGAGG
ATAGCACAGTGCCTCAATTTGAAGCTCCTTCTCCATCACACAGTAGTATTATTGATTCCACAGAATACAGCCAACCTCCAGGTTTCAGTG
GCAGTTCTCAGACAAAGAAACAGCCTTGGTATAATAGCACGTTAGCATCAAGACGAAAACGACTTACTGCTCATTTTGAAGACTTGGAGC
AGTGTTACTTTTCTACAAGGATGTCTCGTATCTCAGATGACAGTCGAACTGCAAGCCAGTTGGATGAATTTCAGGAATGCTTGTCCAAGT
TTACTCGATATAATTCAGTACGACCTTTAGCCACATTGTCATATGCTAGTGATCTCTATAATGGTTCCAGTATAGTCTCTAGTATTGAAT
TTGACCGGGATTGTGACTATTTTGCGATTGCTGGAGTTACAAAGAAGATTAAAGTCTATGAATATGACACTGTCATCCAGGATGCAGTGG
ATATTCATTACCCTGAGAATGAAATGACCTGCAATTCGAAAATCAGCTGTATCAGTTGGAGTAGTTACCATAAGAACCTGTTAGCTAGCA
GTGATTATGAAGGCACTGTTATTTTATGGGATGGATTCACAGGACAGAGGTCAAAGGTCTATCAGGAGCATGAGAAGAGGTGTTGGAGTG
TTGACTTTAATTTGATGGATCCTAAACTCTTGGCTTCAGGTTCTGATGATGCAAAAGTGAAGCTGTGGTCTACCAATCTAGACAACTCAG
TGGCAAGCATTGAGGCAAAGGCTAATGTGTGCTGTGTTAAATTCAGCCCCTCTTCCAGATACCATTTGGCTTTCGGCTGTGCAGATCACT
GTGTCCACTACTATGATCTTCGTAACACTAAACAGCCAATCATGGTATTCAAAGGACACCGTAAAGCAGTCTCTTATGCAAAGTTTGTGA
GTGGTGAGGAAATTGTCTCTGCCTCAACAGACAGTCAGCTAAAACTGTGGAATGTAGGGAAACCATACTGCCTACGTTCCTTCAAGGGTC
ATATCAATGAAAAAAACTTTGTAGGCCTGGCTTCCAATGGAGATTATATAGCTTGTGGAAGTGAAAATAACTCTCTCTACCTGTACTATA
AAGGACTTTCTAAGACTTTGCTAACTTTTAAGTTTGATACAGTCAAAAGTGTTCTCGACAAAGACCGAAAAGAAGATGATACAAATGAAT
TTGTTAGTGCTGTGTGCTGGAGGGCACTACCAGATGGGTTGGAGAATCTAATGCTGGACAAAGATGGCCACATAAAAATTACAGATTTTG
GACTTTGCAAAGAAGGGATCACAGATGCAGCCACCATGAAGACATTCTGTGGCACTCCAGAATATCTGGCACCAGAGGTGTTAGAAGATA
ATGACTATGGCCGAGCAGTAGACTGGTGGGGCCTAGGGGTTGTCATGTATGAAATGATGTGTGGGAGGTTACCTTTCTACAACCAGGACC
ATGAGAAACTTTTTGAATTAATATTAATGGAAGACATTAAATTTCCTCGAACACTCTCTTCAGATGCAAAATCATTGCTTTCAGGGCTCT
TGATAAAGGATCCAAATAAACGCCTTGGTGGAGGACCAGATGATGCAAAAGAAATTATGAGACACAGTTTCTTCTCTGGAGTAAACTGGC
AAGATGTATATGATAAAAAGCTTGTACCTCCTTTTAAACCTCAAGTAACATCTGAGACAGATACTAGATATTTTGATGAAGAATTTACAG
CTCAGACTATTACAATAACACCACCTGAAAAATGTCAGCAATCAGATTGTGGCATGCTGGGTAACTGGAAAAAATAATAAAAATCGGCTT
CCTACAGCCAGCAGCACAGTCACCCATGGAACTGTTGGCTTTGGATTAAATGTGGAATTGAACGACTACCCAGAAGTGTTCTGGAAAGAA
GCGAGATGTGTGGCCTGCCTCACCGTCCTCACCCATCAAAAGCACCAGCAGGCACGTTAACTCGAATTCTCACAAGGAAAAGGCCATTAA

>73668_73668_6_RFWD2-AKT3_RFWD2_chr1_175956078_ENST00000367669_AKT3_chr1_243727150_ENST00000336199_length(amino acids)=903AA_BP=711
MSGSRQAGSGSAGTSPGSSAASSVTSASSSLSSSPSPPSVAVSAAALVSGGVAQAAGSGGLGGPVRPVLVAPAVSGSGGGAVSTGLSRHS
CAARPSAGVGGSSSSLGSGSRKRPLLAPLCNGLINSYEDKSNDFVCPICFDMIEEAYMTKCGHSFCYKCIHQSLEDNNRCPKCNYVVDNI
DHLYPNFLVNELILKQKQRFEEKRFKLDHSVSSTNGHRWQIFQDWLGTDQDNLDLANVNLMLELLVQKKKQLEAESHAAQLQILMEFLKV
ARRNKREQLEQIQKELSVLEEDIKRVEEMSGLYSPVSEDSTVPQFEAPSPSHSSIIDSTEYSQPPGFSGSSQTKKQPWYNSTLASRRKRL
TAHFEDLEQCYFSTRMSRISDDSRTASQLDEFQECLSKFTRYNSVRPLATLSYASDLYNGSSIVSSIEFDRDCDYFAIAGVTKKIKVYEY
DTVIQDAVDIHYPENEMTCNSKISCISWSSYHKNLLASSDYEGTVILWDGFTGQRSKVYQEHEKRCWSVDFNLMDPKLLASGSDDAKVKL
WSTNLDNSVASIEAKANVCCVKFSPSSRYHLAFGCADHCVHYYDLRNTKQPIMVFKGHRKAVSYAKFVSGEEIVSASTDSQLKLWNVGKP
YCLRSFKGHINEKNFVGLASNGDYIACGSENNSLYLYYKGLSKTLLTFKFDTVKSVLDKDRKEDDTNEFVSAVCWRALPDGLENLMLDKD
GHIKITDFGLCKEGITDAATMKTFCGTPEYLAPEVLEDNDYGRAVDWWGLGVVMYEMMCGRLPFYNQDHEKLFELILMEDIKFPRTLSSD
AKSLLSGLLIKDPNKRLGGGPDDAKEIMRHSFFSGVNWQDVYDKKLVPPFKPQVTSETDTRYFDEEFTAQTITITPPEKCQQSDCGMLGN

--------------------------------------------------------------
>73668_73668_7_RFWD2-AKT3_RFWD2_chr1_175956078_ENST00000367669_AKT3_chr1_243727150_ENST00000366539_length(transcript)=6755nt_BP=2648nt
CACCTATACCATTCATTCCAGTCTCCTCAATTCTTTCACTGTATTAAGACGGCCTGCCCTCTGGCACGTTTTCTGGAACGAAAGCGGCTT
GGGACAGACAGACTCCCTTCGCCAGGGAGCCCCGACCCCGAGGGCGCGCCCTGAGCGTCTGAGCGCAGCCCCGCTGCGTCCCCAGTTGTA
TCCCCCGCCACGCCCGCCTCTTCCGCCGCTCGCGCGCGCGCGCACGGCCGGCCACGGCGCGCCGGGGTTGGCTGCTACAGGGACCGCGGT
GGCGGCGGCGGCCTCGACAGCGGTAGTGCCTTCTACTCCGCTTTTTTAGTTTCCTCCGCCCCCTCCCGTGGGACGCTGTTGTGTGGCTGC
CTTAAAAAAAAACGCAACTTTATTGTTCCTCAGCCCCACCTCCGGCTCGGCGGGCGTCCTCAGGATGCACTGAGGCTGAGGGGAGGGGAG
GCGGCGGAGGGTCGAGGTCGCGGTCCCTCTCCTCCGAGCGCCCGGCTGGAGGGGAGGGAGTCACGATGTCTGGTAGCCGCCAGGCCGGGT
CGGGCTCCGCTGGGACAAGCCCCGGGTCCTCGGCGGCCTCCTCGGTGACTTCCGCCTCCTCGTCTTTATCCTCTTCCCCGTCGCCGCCTT
CCGTGGCGGTTTCGGCGGCAGCGCTGGTGTCCGGCGGGGTGGCCCAGGCCGCCGGCTCGGGCGGCCTCGGGGGCCCGGTGCGGCCTGTGT
TGGTGGCGCCCGCCGTATCGGGTAGCGGCGGCGGGGCGGTGTCCACGGGCCTGTCCCGGCACAGCTGCGCGGCCAGGCCCAGCGCCGGCG
TAGGAGGCAGCAGCTCCAGCCTAGGCAGCGGCAGCAGGAAGCGACCTCTCCTCGCCCCCCTCTGCAACGGGCTCATCAACTCCTACGAGG
ACAAAAGCAACGACTTCGTATGCCCCATCTGCTTTGATATGATTGAAGAAGCATACATGACAAAATGTGGCCACAGCTTTTGCTACAAGT
GTATTCATCAGAGTTTGGAGGACAATAATAGATGTCCCAAGTGTAACTATGTTGTGGACAATATTGACCATCTGTATCCTAATTTCTTGG
TGAATGAACTCATTCTTAAACAGAAGCAAAGATTTGAGGAAAAGAGGTTCAAATTGGACCACTCAGTGAGTAGCACCAATGGCCACAGGT
GGCAGATATTTCAAGATTGGTTGGGAACTGACCAAGATAACCTTGATTTGGCCAATGTCAATCTTATGTTGGAGTTACTAGTGCAGAAGA
AGAAACAACTGGAAGCAGAATCACATGCAGCCCAACTACAGATTCTTATGGAATTCCTCAAGGTTGCAAGAAGAAATAAGAGAGAGCAAC
TGGAACAGATCCAGAAGGAGCTAAGTGTTTTGGAAGAGGATATTAAGAGAGTGGAAGAAATGAGTGGCTTATACTCTCCTGTCAGTGAGG
ATAGCACAGTGCCTCAATTTGAAGCTCCTTCTCCATCACACAGTAGTATTATTGATTCCACAGAATACAGCCAACCTCCAGGTTTCAGTG
GCAGTTCTCAGACAAAGAAACAGCCTTGGTATAATAGCACGTTAGCATCAAGACGAAAACGACTTACTGCTCATTTTGAAGACTTGGAGC
AGTGTTACTTTTCTACAAGGATGTCTCGTATCTCAGATGACAGTCGAACTGCAAGCCAGTTGGATGAATTTCAGGAATGCTTGTCCAAGT
TTACTCGATATAATTCAGTACGACCTTTAGCCACATTGTCATATGCTAGTGATCTCTATAATGGTTCCAGTATAGTCTCTAGTATTGAAT
TTGACCGGGATTGTGACTATTTTGCGATTGCTGGAGTTACAAAGAAGATTAAAGTCTATGAATATGACACTGTCATCCAGGATGCAGTGG
ATATTCATTACCCTGAGAATGAAATGACCTGCAATTCGAAAATCAGCTGTATCAGTTGGAGTAGTTACCATAAGAACCTGTTAGCTAGCA
GTGATTATGAAGGCACTGTTATTTTATGGGATGGATTCACAGGACAGAGGTCAAAGGTCTATCAGGAGCATGAGAAGAGGTGTTGGAGTG
TTGACTTTAATTTGATGGATCCTAAACTCTTGGCTTCAGGTTCTGATGATGCAAAAGTGAAGCTGTGGTCTACCAATCTAGACAACTCAG
TGGCAAGCATTGAGGCAAAGGCTAATGTGTGCTGTGTTAAATTCAGCCCCTCTTCCAGATACCATTTGGCTTTCGGCTGTGCAGATCACT
GTGTCCACTACTATGATCTTCGTAACACTAAACAGCCAATCATGGTATTCAAAGGACACCGTAAAGCAGTCTCTTATGCAAAGTTTGTGA
GTGGTGAGGAAATTGTCTCTGCCTCAACAGACAGTCAGCTAAAACTGTGGAATGTAGGGAAACCATACTGCCTACGTTCCTTCAAGGGTC
ATATCAATGAAAAAAACTTTGTAGGCCTGGCTTCCAATGGAGATTATATAGCTTGTGGAAGTGAAAATAACTCTCTCTACCTGTACTATA
AAGGACTTTCTAAGACTTTGCTAACTTTTAAGTTTGATACAGTCAAAAGTGTTCTCGACAAAGACCGAAAAGAAGATGATACAAATGAAT
TTGTTAGTGCTGTGTGCTGGAGGGCACTACCAGATGGGTTGGAGAATCTAATGCTGGACAAAGATGGCCACATAAAAATTACAGATTTTG
GACTTTGCAAAGAAGGGATCACAGATGCAGCCACCATGAAGACATTCTGTGGCACTCCAGAATATCTGGCACCAGAGGTGTTAGAAGATA
ATGACTATGGCCGAGCAGTAGACTGGTGGGGCCTAGGGGTTGTCATGTATGAAATGATGTGTGGGAGGTTACCTTTCTACAACCAGGACC
ATGAGAAACTTTTTGAATTAATATTAATGGAAGACATTAAATTTCCTCGAACACTCTCTTCAGATGCAAAATCATTGCTTTCAGGGCTCT
TGATAAAGGATCCAAATAAACGCCTTGGTGGAGGACCAGATGATGCAAAAGAAATTATGAGACACAGTTTCTTCTCTGGAGTAAACTGGC
AAGATGTATATGATAAAAAGCTTGTACCTCCTTTTAAACCTCAAGTAACATCTGAGACAGATACTAGATATTTTGATGAAGAATTTACAG
CTCAGACTATTACAATAACACCACCTGAAAAATATGATGAGGATGGTATGGACTGCATGGACAATGAGAGGCGGCCGCATTTCCCTCAAT
TTTCCTACTCTGCAAGTGGACGAGAATAAGTCTCTTTCATTCTGCTACTTCACTGTCATCTTCAATTTATTACTGAAAATGATTCCTGGA
CATCACCAGTCCTAGCTCTTACACATAGCAGGGGCACCTTCCGACATCCCAGACCAGCCAAGGGTCCTCACCCCTCGCCACCTTTCACCC
TCATGAAAACACACATACACGCAAATACACTCCAGTTTTTGTTTTTGCATGAAATTGTATCTCAGTCTAAGGTCTCATGCTGTTGCTGCT
ACTGTCTTACTATTATAGCAACTTTAAGAAGTAATTTTCCAACCTTTGGAAGTCATGAGCCCACCATTGTTCATTTGTGCACCAATTATC
ATCTTTTGATCTTTTAGTTTTTCCCTCAGTGAAGGCTAAATGAGATACACTGATTCTAGGTACATTTTTTAACTTTCTAGAAGAGAAAAA
CTAACTAGACTAAGAAGATTTAGTTTATAAATTCAGAACAAGCAATTGTGGAAGGGTGGTGGCGTGCATATGTAAAGCACATCAGATCCG
TGCGTGAAGTAGGCATATATCACTAAGCTGTGGCTGGAATTGATTAGGAAGCATTTGGTAGAAGGACTGAACAACTGTTGGGATATATAT
ATATATATATAATTTTTTTTTTTTAAATTCCTGGTGGATACTGTAGAAGAAGCCCATATCACATGTGGATGTCGAGACTTCACGGGCAAT
CATGAGCAAGTGAACACTGTTCTACCAAGAACTGAAGGCATATGCACAGTCAAGGTCACTTAAAGGGTCTTATGAAACAATTTGAGCCAG
AGAGCATCTTTCCCCTGTGCTTGGAAACCTTTTTTCCTTCTTGACATTTATCACCTCTGATGGCTGAAGAATGTAGACAGGTATAATGAT
ACTGCTTTTCACCAAAATTTCTACACCAAGGTAAACAGGTGTTTGCCTTATTTAATTTTTTACTTTCAGTTCTACGTGAATTAGCTTTTT
CTCAGATGTTGAAACTTTGAATGTCCTTTTATGATTTTGTTTATATTGCAGTAGTATTTATTTTTTAGTGATGAGAATTGTATGTCATGT
TAGCAAACGCAGCTCCAACTTATATAAAATAGACTTACTGCAGTTACTTTTGACCCATGTGCAAGGATTGTACACGCTGATGAGAATCAT
GCACTTTTTCTCCTCTGTTAAAAAAAATGATAAGGCTCTGAAATGGAATATATTGGTTAGAATTTGGCTTTGGGAGAAGAGATGCTGCCA
TTTAACCCCTTGGTACTGAAAATGAGAAAATCCCCAACTATGCATGCCAAGGGGTTAATGAAACAAATAGCTGTTGACGTTTGCTCATTT
AAGAATTTGAAACGTTATGATGACCTGGCAACAAAAAGTAATGAAGAAAATTGAGACCTGAGTGAAGATAAGAAATGATCTTTACGTGGC
AAAATGAACACATCTTGAGTATTTAGGAAATGGGCAGTGAAGGCTAAGAACCTGGTGTGTTTCTTGGGATCATGGTACATTTATCACTGA
ATTAAGCCATCAGGGAAAAAACAACAAAAAAAGAGAACACCTCCAGCTTTTCTTTTTCTGTATATACTCATGTCCCCCAGATTCCAACAT
TTCTCACTGAAAGGGGGCATGTATGCAAACCTCATCTTTCTCCTTCATTAATGATGATCTTCAGATTAAACCCTTTGGTGCTAGGAGCTG
ACAATTTCCAAAGCAGCCTGTGAAGTCCTAGGGGCTGGGGGCCACTCTTGCGGCAAGCAGAAGGCCATCCTACTCCGCGGAGTGATCATG
GAAATGTATTTTAGTTAAACTCTGACAGCTCCCAAACGGAAGACTACAGCATGACGTAGTATTATGATTGCATTGTATGAAAGAGCAAGT
GACTTTCTAAGTAGGATGAATCATATTCATATGCAGATGTCTTAGCCTCTTGACGCTGGAAGTGTGGATTTATAGCTATGAAACCACTGC
TGGCAGTGGGTGGGCCACTGGGACTGACGGGGGTTAAAGGGCATTTTACTAAGGCAGCTAAGACATATTCAGACATCAACGTTATCCTTC
TTTTTCATATTTCTACCTGAGTGAAGTTCATCCTTAGTATTGAGTAGGAAGTTACAGTAAATGGTAGTTCATTCTTACTTACACACATAG
CTAATCTTTTTTTTTTCACTTGGAATTATGTTGAATGTTTCATTTTGACAAAAAAGTAGACTAGAAGGTATGTTCTTTAAGTTGTCTTGC
ATCCATTATATAAGAAAGAAACAGGTGAGAGGAAGAGCAGAAAGCTGAGACTGGCTGATGTTCAGAGCACTTACTCCTCTAGAGGGAAAG
CATGACACCGAACACTAAGCACACAGCTTTTTGTTGTTTTGGTTTTTTCTCCCGCAAATCTTAAAGTGATTCCCATGACCTTGGCCAAGG
ACACTTCTTAAAGATTAATGACTGGCACTGACATTGCCCCAGGCGGGCCACTCCTCACACTGGCTCTCAGTTCCCAGCCATGCCTGGGGC
TCAGTCACTTCTATTCCACCCTCTGAGACTCCATTGGTGTCACACAAGGTGTCTTCTTGGCTTTGATTTTGAGAATCCCCTATTTTCACT
TCCAGATCTGTCAGCTGCCATGGAGGAATAATAGAAAACCAGAAATGCGTGTAGAGGGAGATTTCTAAAACTTCCCTTGTGTCGCCCATA
GTTGTAGTTTTGGGTTCTGGCAGGTGGAACACCCTGAAACCTGGAATCATTCTATGAGAATACAGTTCAGACTTTGCAGACTCCAGCCCA
TACTAACTGTCATGAAGCTTGACTTCTTGTCATAATGCAGCCATCTTGGAGGAAATTGGCCATTTCTGCTTAGATGGTTGGCAGGGTCGC
GCTCAGCTTTGCTTTCTACACTAATTACATAGCATTATTCAAGTATTGTTTTCCATTTCCCATCCCTGATTTCCAGCTTCTTAAAGCTGA
CTGTTCTTGCAGGGGCCACTTGCTTCTCCTAGAGTACAAAAGTAAGGGCCTTCCTTACTAACTGCAGGGTCTCTCTATTACACCTCAACA
TACACACTTTGCTGCTACTGTTTGTACTGTCTACAGTAGAATTTCCTTATCTTGCTCCTGGTAGTGCATTACAGGCAAGCATGAAATGTA
AAGTATTTATTTAAATAAAAAGAAAACCTCTAAATTGGTAATTGAATTACCTCCCTGTAGCTTTATAGTTTGTGACATTTCTTGACCTTG
CTAGTTCTTTCATTAGATCTGCGCAAGATCTAGTCATCTGGTTAAGGATTTTAAGCAGATGCAACTATAAACCCAAGAAACTGTATTACT
ATTACTGTTGGTCATACTAAACCTGTCTATTTCCTGAAGTATATGACCCACAAGGATGTGGAATAACTAGGAGAAACTGTTTTTGTACAC
TGTACATCCTTAGTATTTTTACACGTATATGATAGGGATGAACATGATTTTCCTTCGTACAGACAGCTTAAATAAAGCACTATGTCAATC

>73668_73668_7_RFWD2-AKT3_RFWD2_chr1_175956078_ENST00000367669_AKT3_chr1_243727150_ENST00000366539_length(amino acids)=917AA_BP=711
MSGSRQAGSGSAGTSPGSSAASSVTSASSSLSSSPSPPSVAVSAAALVSGGVAQAAGSGGLGGPVRPVLVAPAVSGSGGGAVSTGLSRHS
CAARPSAGVGGSSSSLGSGSRKRPLLAPLCNGLINSYEDKSNDFVCPICFDMIEEAYMTKCGHSFCYKCIHQSLEDNNRCPKCNYVVDNI
DHLYPNFLVNELILKQKQRFEEKRFKLDHSVSSTNGHRWQIFQDWLGTDQDNLDLANVNLMLELLVQKKKQLEAESHAAQLQILMEFLKV
ARRNKREQLEQIQKELSVLEEDIKRVEEMSGLYSPVSEDSTVPQFEAPSPSHSSIIDSTEYSQPPGFSGSSQTKKQPWYNSTLASRRKRL
TAHFEDLEQCYFSTRMSRISDDSRTASQLDEFQECLSKFTRYNSVRPLATLSYASDLYNGSSIVSSIEFDRDCDYFAIAGVTKKIKVYEY
DTVIQDAVDIHYPENEMTCNSKISCISWSSYHKNLLASSDYEGTVILWDGFTGQRSKVYQEHEKRCWSVDFNLMDPKLLASGSDDAKVKL
WSTNLDNSVASIEAKANVCCVKFSPSSRYHLAFGCADHCVHYYDLRNTKQPIMVFKGHRKAVSYAKFVSGEEIVSASTDSQLKLWNVGKP
YCLRSFKGHINEKNFVGLASNGDYIACGSENNSLYLYYKGLSKTLLTFKFDTVKSVLDKDRKEDDTNEFVSAVCWRALPDGLENLMLDKD
GHIKITDFGLCKEGITDAATMKTFCGTPEYLAPEVLEDNDYGRAVDWWGLGVVMYEMMCGRLPFYNQDHEKLFELILMEDIKFPRTLSSD
AKSLLSGLLIKDPNKRLGGGPDDAKEIMRHSFFSGVNWQDVYDKKLVPPFKPQVTSETDTRYFDEEFTAQTITITPPEKYDEDGMDCMDN

--------------------------------------------------------------
>73668_73668_8_RFWD2-AKT3_RFWD2_chr1_175956078_ENST00000367669_AKT3_chr1_243727150_ENST00000366540_length(transcript)=3251nt_BP=2648nt
CACCTATACCATTCATTCCAGTCTCCTCAATTCTTTCACTGTATTAAGACGGCCTGCCCTCTGGCACGTTTTCTGGAACGAAAGCGGCTT
GGGACAGACAGACTCCCTTCGCCAGGGAGCCCCGACCCCGAGGGCGCGCCCTGAGCGTCTGAGCGCAGCCCCGCTGCGTCCCCAGTTGTA
TCCCCCGCCACGCCCGCCTCTTCCGCCGCTCGCGCGCGCGCGCACGGCCGGCCACGGCGCGCCGGGGTTGGCTGCTACAGGGACCGCGGT
GGCGGCGGCGGCCTCGACAGCGGTAGTGCCTTCTACTCCGCTTTTTTAGTTTCCTCCGCCCCCTCCCGTGGGACGCTGTTGTGTGGCTGC
CTTAAAAAAAAACGCAACTTTATTGTTCCTCAGCCCCACCTCCGGCTCGGCGGGCGTCCTCAGGATGCACTGAGGCTGAGGGGAGGGGAG
GCGGCGGAGGGTCGAGGTCGCGGTCCCTCTCCTCCGAGCGCCCGGCTGGAGGGGAGGGAGTCACGATGTCTGGTAGCCGCCAGGCCGGGT
CGGGCTCCGCTGGGACAAGCCCCGGGTCCTCGGCGGCCTCCTCGGTGACTTCCGCCTCCTCGTCTTTATCCTCTTCCCCGTCGCCGCCTT
CCGTGGCGGTTTCGGCGGCAGCGCTGGTGTCCGGCGGGGTGGCCCAGGCCGCCGGCTCGGGCGGCCTCGGGGGCCCGGTGCGGCCTGTGT
TGGTGGCGCCCGCCGTATCGGGTAGCGGCGGCGGGGCGGTGTCCACGGGCCTGTCCCGGCACAGCTGCGCGGCCAGGCCCAGCGCCGGCG
TAGGAGGCAGCAGCTCCAGCCTAGGCAGCGGCAGCAGGAAGCGACCTCTCCTCGCCCCCCTCTGCAACGGGCTCATCAACTCCTACGAGG
ACAAAAGCAACGACTTCGTATGCCCCATCTGCTTTGATATGATTGAAGAAGCATACATGACAAAATGTGGCCACAGCTTTTGCTACAAGT
GTATTCATCAGAGTTTGGAGGACAATAATAGATGTCCCAAGTGTAACTATGTTGTGGACAATATTGACCATCTGTATCCTAATTTCTTGG
TGAATGAACTCATTCTTAAACAGAAGCAAAGATTTGAGGAAAAGAGGTTCAAATTGGACCACTCAGTGAGTAGCACCAATGGCCACAGGT
GGCAGATATTTCAAGATTGGTTGGGAACTGACCAAGATAACCTTGATTTGGCCAATGTCAATCTTATGTTGGAGTTACTAGTGCAGAAGA
AGAAACAACTGGAAGCAGAATCACATGCAGCCCAACTACAGATTCTTATGGAATTCCTCAAGGTTGCAAGAAGAAATAAGAGAGAGCAAC
TGGAACAGATCCAGAAGGAGCTAAGTGTTTTGGAAGAGGATATTAAGAGAGTGGAAGAAATGAGTGGCTTATACTCTCCTGTCAGTGAGG
ATAGCACAGTGCCTCAATTTGAAGCTCCTTCTCCATCACACAGTAGTATTATTGATTCCACAGAATACAGCCAACCTCCAGGTTTCAGTG
GCAGTTCTCAGACAAAGAAACAGCCTTGGTATAATAGCACGTTAGCATCAAGACGAAAACGACTTACTGCTCATTTTGAAGACTTGGAGC
AGTGTTACTTTTCTACAAGGATGTCTCGTATCTCAGATGACAGTCGAACTGCAAGCCAGTTGGATGAATTTCAGGAATGCTTGTCCAAGT
TTACTCGATATAATTCAGTACGACCTTTAGCCACATTGTCATATGCTAGTGATCTCTATAATGGTTCCAGTATAGTCTCTAGTATTGAAT
TTGACCGGGATTGTGACTATTTTGCGATTGCTGGAGTTACAAAGAAGATTAAAGTCTATGAATATGACACTGTCATCCAGGATGCAGTGG
ATATTCATTACCCTGAGAATGAAATGACCTGCAATTCGAAAATCAGCTGTATCAGTTGGAGTAGTTACCATAAGAACCTGTTAGCTAGCA
GTGATTATGAAGGCACTGTTATTTTATGGGATGGATTCACAGGACAGAGGTCAAAGGTCTATCAGGAGCATGAGAAGAGGTGTTGGAGTG
TTGACTTTAATTTGATGGATCCTAAACTCTTGGCTTCAGGTTCTGATGATGCAAAAGTGAAGCTGTGGTCTACCAATCTAGACAACTCAG
TGGCAAGCATTGAGGCAAAGGCTAATGTGTGCTGTGTTAAATTCAGCCCCTCTTCCAGATACCATTTGGCTTTCGGCTGTGCAGATCACT
GTGTCCACTACTATGATCTTCGTAACACTAAACAGCCAATCATGGTATTCAAAGGACACCGTAAAGCAGTCTCTTATGCAAAGTTTGTGA
GTGGTGAGGAAATTGTCTCTGCCTCAACAGACAGTCAGCTAAAACTGTGGAATGTAGGGAAACCATACTGCCTACGTTCCTTCAAGGGTC
ATATCAATGAAAAAAACTTTGTAGGCCTGGCTTCCAATGGAGATTATATAGCTTGTGGAAGTGAAAATAACTCTCTCTACCTGTACTATA
AAGGACTTTCTAAGACTTTGCTAACTTTTAAGTTTGATACAGTCAAAAGTGTTCTCGACAAAGACCGAAAAGAAGATGATACAAATGAAT
TTGTTAGTGCTGTGTGCTGGAGGGCACTACCAGATGGGTTGGAGAATCTAATGCTGGACAAAGATGGCCACATAAAAATTACAGATTTTG
GACTTTGCAAAGAAGGGATCACAGATGCAGCCACCATGAAGACATTCTGTGGCACTCCAGAATATCTGGCACCAGAGGTGTTAGAAGATA
ATGACTATGGCCGAGCAGTAGACTGGTGGGGCCTAGGGGTTGTCATGTATGAAATGATGTGTGGGAGGTTACCTTTCTACAACCAGGACC
ATGAGAAACTTTTTGAATTAATATTAATGGAAGACATTAAATTTCCTCGAACACTCTCTTCAGATGCAAAATCATTGCTTTCAGGGCTCT
TGATAAAGGATCCAAATAAACGCCTTGGTGGAGGACCAGATGATGCAAAAGAAATTATGAGACACAGTTTCTTCTCTGGAGTAAACTGGC
AAGATGTATATGATAAAAAGCTTGTACCTCCTTTTAAACCTCAAGTAACATCTGAGACAGATACTAGATATTTTGATGAAGAATTTACAG
CTCAGACTATTACAATAACACCACCTGAAAAATGTCAGCAATCAGATTGTGGCATGCTGGGTAACTGGAAAAAATAATAAAAAGTAAGTT

>73668_73668_8_RFWD2-AKT3_RFWD2_chr1_175956078_ENST00000367669_AKT3_chr1_243727150_ENST00000366540_length(amino acids)=903AA_BP=711
MSGSRQAGSGSAGTSPGSSAASSVTSASSSLSSSPSPPSVAVSAAALVSGGVAQAAGSGGLGGPVRPVLVAPAVSGSGGGAVSTGLSRHS
CAARPSAGVGGSSSSLGSGSRKRPLLAPLCNGLINSYEDKSNDFVCPICFDMIEEAYMTKCGHSFCYKCIHQSLEDNNRCPKCNYVVDNI
DHLYPNFLVNELILKQKQRFEEKRFKLDHSVSSTNGHRWQIFQDWLGTDQDNLDLANVNLMLELLVQKKKQLEAESHAAQLQILMEFLKV
ARRNKREQLEQIQKELSVLEEDIKRVEEMSGLYSPVSEDSTVPQFEAPSPSHSSIIDSTEYSQPPGFSGSSQTKKQPWYNSTLASRRKRL
TAHFEDLEQCYFSTRMSRISDDSRTASQLDEFQECLSKFTRYNSVRPLATLSYASDLYNGSSIVSSIEFDRDCDYFAIAGVTKKIKVYEY
DTVIQDAVDIHYPENEMTCNSKISCISWSSYHKNLLASSDYEGTVILWDGFTGQRSKVYQEHEKRCWSVDFNLMDPKLLASGSDDAKVKL
WSTNLDNSVASIEAKANVCCVKFSPSSRYHLAFGCADHCVHYYDLRNTKQPIMVFKGHRKAVSYAKFVSGEEIVSASTDSQLKLWNVGKP
YCLRSFKGHINEKNFVGLASNGDYIACGSENNSLYLYYKGLSKTLLTFKFDTVKSVLDKDRKEDDTNEFVSAVCWRALPDGLENLMLDKD
GHIKITDFGLCKEGITDAATMKTFCGTPEYLAPEVLEDNDYGRAVDWWGLGVVMYEMMCGRLPFYNQDHEKLFELILMEDIKFPRTLSSD
AKSLLSGLLIKDPNKRLGGGPDDAKEIMRHSFFSGVNWQDVYDKKLVPPFKPQVTSETDTRYFDEEFTAQTITITPPEKCQQSDCGMLGN

--------------------------------------------------------------

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Fusion Gene PPI Analysis for RFWD2-AKT3


check button Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in

ChiPPI page.


check button Protein-protein interactors with each fusion partner protein in wild-type (BIOGRID-3.4.160)
HgeneHgene's interactorsTgeneTgene's interactors


check button - Retained PPIs in in-frame fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenStill interaction with
HgeneRFWD2chr1:175956078chr1:243727150ENST00000308769-1719643_645687.0708.0TRIB1
HgeneRFWD2chr1:175956078chr1:243727150ENST00000367669-1820643_645711.0732.0TRIB1


check button - Lost PPIs in in-frame fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenInteraction lost with


check button - Retained PPIs, but lost function due to frame-shift fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenInteraction lost with


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Related Drugs for RFWD2-AKT3


check button Drugs targeting genes involved in this fusion gene.
(DrugBank Version 5.1.8 2021-05-08)
PartnerGeneUniProtAccDrugBank IDDrug nameDrug activityDrug typeDrug status

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Related Diseases for RFWD2-AKT3


check button Diseases associated with fusion partners.
(DisGeNet 4.0)
PartnerGeneDisease IDDisease name# pubmedsSource
TgeneC4014738MEGALENCEPHALY-POLYMICROGYRIA-POLYDACTYLY-HYDROCEPHALUS SYNDROME 24GENOMICS_ENGLAND;UNIPROT
TgeneC4012727MEGALENCEPHALY-POLYMICROGYRIA-POLYDACTYLY-HYDROCEPHALUS SYNDROME 12GENOMICS_ENGLAND
TgeneC0152427Polydactyly1GENOMICS_ENGLAND
TgeneC0431380Cortical Dysplasia1CTD_human
TgeneC0431391Hemimegalencephaly1ORPHANET
TgeneC1863924Megalanecephaly Polymicrogyria-Polydactyly Hydrocephalus Syndrome1CTD_human;ORPHANET
TgeneC1955869Malformations of Cortical Development1CTD_human