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Center for Computational Systems Medicine
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Fusion Gene Summary

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Fusion Gene ORF analysis

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Fusion Genomic Features

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Fusion Protein Features

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Fusion Gene Sequence

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Fusion Gene PPI analysis

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Related Drugs

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Related Diseases

Fusion gene:AIDA-MIA3 (FusionGDB2 ID:HG64853TG375056)

Fusion Gene Summary for AIDA-MIA3

check button Fusion gene summary
Fusion gene informationFusion gene name: AIDA-MIA3
Fusion gene ID: hg64853tg375056
HgeneTgene
Gene symbol

AIDA

MIA3

Gene ID

64853

375056

Gene nameaxin interactor, dorsalization associatedMIA SH3 domain ER export factor 3
SynonymsC1orf80ARNT|D320|TANGO|TANGO1|UNQ6077
Cytomap('AIDA')('MIA3')

1q41

1q41

Type of geneprotein-codingprotein-coding
Descriptionaxin interactor, dorsalization-associated proteinaxin interaction partner and dorsalization antagonisttransport and Golgi organization protein 1 homologC219-reactive peptideMIA family member 3, ER export factormelanoma inhibitory activity family, member 3melanoma inhibitory activity protein 3transport and Golgi organization protein 1
Modification date2020032720200313
UniProtAcc..
Ensembl transtripts involved in fusion geneENST00000340020, ENST00000355727, 
ENST00000541237, ENST00000474863, 
Fusion gene scores* DoF score3 X 3 X 3=274 X 5 X 3=60
# samples 35
** MAII scorelog2(3/27*10)=0.15200309344505
effective Gene in Pan-Cancer Fusion Genes (eGinPCFGs).
DoF>8 and MAII>0
log2(5/60*10)=-0.263034405833794
possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs).
DoF>8 and MAII<0
Context

PubMed: AIDA [Title/Abstract] AND MIA3 [Title/Abstract] AND fusion [Title/Abstract]

Most frequent breakpointAIDA(222867131)-MIA3(222822171), # samples:1
Anticipated loss of major functional domain due to fusion event.AIDA-MIA3 seems lost the major protein functional domain in Tgene partner, which is a essential gene due to the frame-shifted ORF.
* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

check button Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez
PartnerGeneGO IDGO termPubMed ID
HgeneAIDA

GO:0043508

negative regulation of JUN kinase activity

17681137

TgeneMIA3

GO:0002687

positive regulation of leukocyte migration

17726152

TgeneMIA3

GO:0007162

negative regulation of cell adhesion

17726152

TgeneMIA3

GO:0030336

negative regulation of cell migration

17044017

TgeneMIA3

GO:0042060

wound healing

17044017



check button Fusion gene information
* All genome coordinats were lifted-over on hg19.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.
SourceDiseaseSampleHgeneHchrHbpHstrandTgeneTchrTbpTstrand
ChimerDB4BRCATCGA-AR-A1AR-01AAIDAchr1

222867131

-MIA3chr1

222822171

+


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Fusion Gene ORF analysis for AIDA-MIA3

check button Open reading frame (ORF) analsis of fusion genes based on Ensembl gene isoform structure.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.
ORFHenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrand
5CDS-intronENST00000340020ENST00000340535AIDAchr1

222867131

-MIA3chr1

222822171

+
5CDS-intronENST00000340020ENST00000344441AIDAchr1

222867131

-MIA3chr1

222822171

+
5CDS-intronENST00000340020ENST00000344507AIDAchr1

222867131

-MIA3chr1

222822171

+
5CDS-intronENST00000340020ENST00000470521AIDAchr1

222867131

-MIA3chr1

222822171

+
5CDS-intronENST00000355727ENST00000340535AIDAchr1

222867131

-MIA3chr1

222822171

+
5CDS-intronENST00000355727ENST00000344441AIDAchr1

222867131

-MIA3chr1

222822171

+
5CDS-intronENST00000355727ENST00000344507AIDAchr1

222867131

-MIA3chr1

222822171

+
5CDS-intronENST00000355727ENST00000470521AIDAchr1

222867131

-MIA3chr1

222822171

+
5CDS-intronENST00000541237ENST00000340535AIDAchr1

222867131

-MIA3chr1

222822171

+
5CDS-intronENST00000541237ENST00000344441AIDAchr1

222867131

-MIA3chr1

222822171

+
5CDS-intronENST00000541237ENST00000344507AIDAchr1

222867131

-MIA3chr1

222822171

+
5CDS-intronENST00000541237ENST00000470521AIDAchr1

222867131

-MIA3chr1

222822171

+
5UTR-3CDSENST00000474863ENST00000344922AIDAchr1

222867131

-MIA3chr1

222822171

+
5UTR-intronENST00000474863ENST00000340535AIDAchr1

222867131

-MIA3chr1

222822171

+
5UTR-intronENST00000474863ENST00000344441AIDAchr1

222867131

-MIA3chr1

222822171

+
5UTR-intronENST00000474863ENST00000344507AIDAchr1

222867131

-MIA3chr1

222822171

+
5UTR-intronENST00000474863ENST00000470521AIDAchr1

222867131

-MIA3chr1

222822171

+
Frame-shiftENST00000340020ENST00000344922AIDAchr1

222867131

-MIA3chr1

222822171

+
Frame-shiftENST00000355727ENST00000344922AIDAchr1

222867131

-MIA3chr1

222822171

+
Frame-shiftENST00000541237ENST00000344922AIDAchr1

222867131

-MIA3chr1

222822171

+

check buttonORFfinder result based on the fusion transcript sequence of in-frame fusion genes.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandSeq length
(transcript)
BP loci
(transcript)
Predicted start
(transcript)
Predicted stop
(transcript)
Seq length
(amino acids)

check buttonDeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandNo-coding scoreCoding score

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Fusion Genomic Features for AIDA-MIA3


check buttonFusionAI prediction of the potential fusion gene breakpoint based on the pre-mature RNA sequence context (+/- 5kb of individual partner genes, total 20kb length sequence). FusionAI is a fusion gene breakpoint classifier based on convolutional neural network by comparing the fusion positive and negative sequence context of ~ 20K fusion gene data. From here, we can have the relative potentency of the 20K genomic sequence how individual sequnce will be likely used as the gene fusion breakpoints.
HgeneHchrHbpHstrandTgeneTchrTbpTstrand1-pp (fusion gene breakpoint)
AIDAchr1222867130-MIA3chr1222822170+1.36E-050.9999864
AIDAchr1222867130-MIA3chr1222822170+1.36E-050.9999864


check buttonDistribution of 44 human genomic features loci across 20kb length fusion breakpoint regions that are ovelapped with the top 1% feature importance score regions. More details are in help page.
genomic feature of top 1%

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Fusion Protein Features for AIDA-MIA3


check button Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/:222867131/:222822171)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
4. Go down and press 'Search' tab twice.
4. Go down to have the hyperlink of the search result.
5. Click the hyperlink.
6. See the FGviewer result for your fusion gene.
FGviewer

check buttonMain function of each fusion partner protein. (from UniProt)
HgeneTgene
..
FUNCTION: Transcriptional activator which is required for calcium-dependent dendritic growth and branching in cortical neurons. Recruits CREB-binding protein (CREBBP) to nuclear bodies. Component of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating a calcium-dependent release of a repressor complex and a recruitment of an activator complex. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex. At the same time, there is increased recruitment of CREBBP to the promoter by a CREST-dependent mechanism, which leads to transcriptional activation. The CREST-BRG1 complex also binds to the NR2B promoter, and activity-dependent induction of NR2B expression involves a release of HDAC1 and recruitment of CREBBP (By similarity). {ECO:0000250}.FUNCTION: Transcriptional activator which is required for calcium-dependent dendritic growth and branching in cortical neurons. Recruits CREB-binding protein (CREBBP) to nuclear bodies. Component of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating a calcium-dependent release of a repressor complex and a recruitment of an activator complex. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex. At the same time, there is increased recruitment of CREBBP to the promoter by a CREST-dependent mechanism, which leads to transcriptional activation. The CREST-BRG1 complex also binds to the NR2B promoter, and activity-dependent induction of NR2B expression involves a release of HDAC1 and recruitment of CREBBP (By similarity). {ECO:0000250}.

check buttonRetention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at

download page


* Minus value of BPloci means that the break pointn is located before the CDS.
- In-frame and retained protein feature among the 13 regional features.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note

- In-frame and not-retained protein feature among the 13 regional features.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note


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Fusion Gene Sequence for AIDA-MIA3


check button For in-frame fusion transcripts, we provide the fusion transcript sequences and fusion amino acid sequences. To have fusion amino acid sequence, we ran ORFfinder and chose the longest ORF among the all predicted ones.

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Fusion Gene PPI Analysis for AIDA-MIA3


check button Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in

ChiPPI page.


check button Protein-protein interactors with each fusion partner protein in wild-type (BIOGRID-3.4.160)
HgeneHgene's interactorsTgeneTgene's interactors


check button - Retained PPIs in in-frame fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenStill interaction with


check button - Lost PPIs in in-frame fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenInteraction lost with


check button - Retained PPIs, but lost function due to frame-shift fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenInteraction lost with


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Related Drugs for AIDA-MIA3


check button Drugs targeting genes involved in this fusion gene.
(DrugBank Version 5.1.8 2021-05-08)
PartnerGeneUniProtAccDrugBank IDDrug nameDrug activityDrug typeDrug status

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Related Diseases for AIDA-MIA3


check button Diseases associated with fusion partners.
(DisGeNet 4.0)
PartnerGeneDisease IDDisease name# pubmedsSource
HgeneAIDAC0023893Liver Cirrhosis, Experimental1CTD_human
TgeneC0010054Coronary Arteriosclerosis1CTD_human
TgeneC0027051Myocardial Infarction1CTD_human
TgeneC1956346Coronary Artery Disease1CTD_human