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	Fusion Gene Summary
	Fusion Gene ORF analysis
	Fusion Genomic Features
	Fusion Protein Features
	Fusion Gene Sequence
	Fusion Gene PPI analysis
	Related Drugs
	Related Diseases

Fusion gene:CARS-ALK (FusionGDB2 ID:HG833TG238)

Fusion Gene Summary for CARS-ALK

Fusion gene summary

Fusion gene information	Fusion gene name: CARS-ALK
	Fusion gene ID: hg833tg238
		Hgene	Tgene
	Gene symbol	CARS	ALK
	Gene ID	833	238
	Gene name	cysteinyl-tRNA synthetase 1	ALK receptor tyrosine kinase
	Synonyms	CARS\|CYSRS\|MGC:11246	CD246\|NBLST3
	Cytomap	('CARS')('ALK') 11p15.4	2p23.2-p23.1
	Type of gene	protein-coding	protein-coding
	Description	cysteine--tRNA ligase, cytoplasmiccysteine tRNA ligase 1, cytoplasmiccysteine translase	ALK tyrosine kinase receptorCD246 antigenanaplastic lymphoma receptor tyrosine kinasemutant anaplastic lymphoma kinase
	Modification date	20200313	20200329
	UniProtAcc	.	Q9UM73
	Ensembl transtripts involved in fusion gene	ENST00000278224, ENST00000380525, ENST00000397111, ENST00000397114, ENST00000401769, ENST00000470221,
Fusion gene scores	* DoF score	9 X 11 X 6=594	56 X 74 X 20=82880
	# samples	11	57
	** MAII score	log2(11/594*10)=-2.43295940727611 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0	log2(57/82880*10)=-7.18391827352181 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0
Context	PubMed: CARS [Title/Abstract] AND ALK [Title/Abstract] AND fusion [Title/Abstract]
Most frequent breakpoint
Anticipated loss of major functional domain due to fusion event.	CARS-ALK seems lost the major protein functional domain in Hgene partner, which is a CGC due to the frame-shifted ORF. CARS-ALK seems lost the major protein functional domain in Hgene partner, which is a essential gene due to the frame-shifted ORF. CARS-ALK seems lost the major protein functional domain in Tgene partner, which is a CGC due to the frame-shifted ORF. CARS-ALK seems lost the major protein functional domain in Tgene partner, which is a IUPHAR drug target due to the frame-shifted ORF. CARS-ALK seems lost the major protein functional domain in Tgene partner, which is a kinase due to the frame-shifted ORF.

* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez

Partner	Gene	GO ID	GO term	PubMed ID
Hgene	CARS	GO:0006423	cysteinyl-tRNA aminoacylation	10908348\|17303165
Tgene	ALK	GO:0016310	phosphorylation	9174053
Tgene	ALK	GO:0046777	protein autophosphorylation	9174053

Fusion gene information
* All genome coordinats were lifted-over on hg19.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.

Source	Disease	Sample	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand
ChimerKB3	.	.	CARS	chr11	3033424	-	ALK	chr2	29446394	-
ChimerKB3	.	.	CARS	chr11	3037010	-	ALK	chr2	29446394	-
ChimerKB3	.	.	CARS	chr11	3038335	-	ALK	chr2	29445473	-

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Fusion Gene ORF analysis for CARS-ALK

Open reading frame (ORF) analsis of fusion genes based on Ensembl gene isoform structure.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.

ORF	Henst	Tenst	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand
5CDS-intron	ENST00000278224	ENST00000431873	CARS	chr11	3033424	-	ALK	chr2	29446394	-
5CDS-intron	ENST00000278224	ENST00000431873	CARS	chr11	3037010	-	ALK	chr2	29446394	-
5CDS-intron	ENST00000278224	ENST00000431873	CARS	chr11	3038335	-	ALK	chr2	29445473	-
5CDS-intron	ENST00000278224	ENST00000498037	CARS	chr11	3033424	-	ALK	chr2	29446394	-
5CDS-intron	ENST00000278224	ENST00000498037	CARS	chr11	3037010	-	ALK	chr2	29446394	-
5CDS-intron	ENST00000278224	ENST00000498037	CARS	chr11	3038335	-	ALK	chr2	29445473	-
5CDS-intron	ENST00000380525	ENST00000431873	CARS	chr11	3033424	-	ALK	chr2	29446394	-
5CDS-intron	ENST00000380525	ENST00000431873	CARS	chr11	3037010	-	ALK	chr2	29446394	-
5CDS-intron	ENST00000380525	ENST00000431873	CARS	chr11	3038335	-	ALK	chr2	29445473	-
5CDS-intron	ENST00000380525	ENST00000498037	CARS	chr11	3033424	-	ALK	chr2	29446394	-
5CDS-intron	ENST00000380525	ENST00000498037	CARS	chr11	3037010	-	ALK	chr2	29446394	-
5CDS-intron	ENST00000380525	ENST00000498037	CARS	chr11	3038335	-	ALK	chr2	29445473	-
5CDS-intron	ENST00000397111	ENST00000431873	CARS	chr11	3033424	-	ALK	chr2	29446394	-
5CDS-intron	ENST00000397111	ENST00000431873	CARS	chr11	3037010	-	ALK	chr2	29446394	-
5CDS-intron	ENST00000397111	ENST00000431873	CARS	chr11	3038335	-	ALK	chr2	29445473	-
5CDS-intron	ENST00000397111	ENST00000498037	CARS	chr11	3033424	-	ALK	chr2	29446394	-
5CDS-intron	ENST00000397111	ENST00000498037	CARS	chr11	3037010	-	ALK	chr2	29446394	-
5CDS-intron	ENST00000397111	ENST00000498037	CARS	chr11	3038335	-	ALK	chr2	29445473	-
5CDS-intron	ENST00000397114	ENST00000431873	CARS	chr11	3033424	-	ALK	chr2	29446394	-
5CDS-intron	ENST00000397114	ENST00000431873	CARS	chr11	3037010	-	ALK	chr2	29446394	-
5CDS-intron	ENST00000397114	ENST00000431873	CARS	chr11	3038335	-	ALK	chr2	29445473	-
5CDS-intron	ENST00000397114	ENST00000498037	CARS	chr11	3033424	-	ALK	chr2	29446394	-
5CDS-intron	ENST00000397114	ENST00000498037	CARS	chr11	3037010	-	ALK	chr2	29446394	-
5CDS-intron	ENST00000397114	ENST00000498037	CARS	chr11	3038335	-	ALK	chr2	29445473	-
5CDS-intron	ENST00000401769	ENST00000431873	CARS	chr11	3033424	-	ALK	chr2	29446394	-
5CDS-intron	ENST00000401769	ENST00000431873	CARS	chr11	3037010	-	ALK	chr2	29446394	-
5CDS-intron	ENST00000401769	ENST00000431873	CARS	chr11	3038335	-	ALK	chr2	29445473	-
5CDS-intron	ENST00000401769	ENST00000498037	CARS	chr11	3033424	-	ALK	chr2	29446394	-
5CDS-intron	ENST00000401769	ENST00000498037	CARS	chr11	3037010	-	ALK	chr2	29446394	-
5CDS-intron	ENST00000401769	ENST00000498037	CARS	chr11	3038335	-	ALK	chr2	29445473	-
Frame-shift	ENST00000278224	ENST00000389048	CARS	chr11	3037010	-	ALK	chr2	29446394	-
Frame-shift	ENST00000278224	ENST00000389048	CARS	chr11	3038335	-	ALK	chr2	29445473	-
Frame-shift	ENST00000380525	ENST00000389048	CARS	chr11	3037010	-	ALK	chr2	29446394	-
Frame-shift	ENST00000380525	ENST00000389048	CARS	chr11	3038335	-	ALK	chr2	29445473	-
Frame-shift	ENST00000397111	ENST00000389048	CARS	chr11	3037010	-	ALK	chr2	29446394	-
Frame-shift	ENST00000397111	ENST00000389048	CARS	chr11	3038335	-	ALK	chr2	29445473	-
Frame-shift	ENST00000397114	ENST00000389048	CARS	chr11	3033424	-	ALK	chr2	29446394	-
Frame-shift	ENST00000397114	ENST00000389048	CARS	chr11	3038335	-	ALK	chr2	29445473	-
Frame-shift	ENST00000401769	ENST00000389048	CARS	chr11	3033424	-	ALK	chr2	29446394	-
Frame-shift	ENST00000401769	ENST00000389048	CARS	chr11	3038335	-	ALK	chr2	29445473	-
In-frame	ENST00000278224	ENST00000389048	CARS	chr11	3033424	-	ALK	chr2	29446394	-
In-frame	ENST00000380525	ENST00000389048	CARS	chr11	3033424	-	ALK	chr2	29446394	-
In-frame	ENST00000397111	ENST00000389048	CARS	chr11	3033424	-	ALK	chr2	29446394	-
In-frame	ENST00000397114	ENST00000389048	CARS	chr11	3037010	-	ALK	chr2	29446394	-
In-frame	ENST00000401769	ENST00000389048	CARS	chr11	3037010	-	ALK	chr2	29446394	-
intron-3CDS	ENST00000470221	ENST00000389048	CARS	chr11	3033424	-	ALK	chr2	29446394	-
intron-3CDS	ENST00000470221	ENST00000389048	CARS	chr11	3037010	-	ALK	chr2	29446394	-
intron-3CDS	ENST00000470221	ENST00000389048	CARS	chr11	3038335	-	ALK	chr2	29445473	-
intron-intron	ENST00000470221	ENST00000431873	CARS	chr11	3033424	-	ALK	chr2	29446394	-
intron-intron	ENST00000470221	ENST00000431873	CARS	chr11	3037010	-	ALK	chr2	29446394	-
intron-intron	ENST00000470221	ENST00000431873	CARS	chr11	3038335	-	ALK	chr2	29445473	-
intron-intron	ENST00000470221	ENST00000498037	CARS	chr11	3033424	-	ALK	chr2	29446394	-
intron-intron	ENST00000470221	ENST00000498037	CARS	chr11	3037010	-	ALK	chr2	29446394	-
intron-intron	ENST00000470221	ENST00000498037	CARS	chr11	3038335	-	ALK	chr2	29445473	-

ORFfinder result based on the fusion transcript sequence of in-frame fusion genes.

Henst	Tenst	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	Seq length (transcript)	BP loci (transcript)	Predicted start (transcript)	Predicted stop (transcript)	Seq length (amino acids)
ENST00000380525	CARS	chr11	3033424	-	ENST00000389048	ALK	chr2	29446394	-	4279	2138	70	3828	1252
ENST00000397111	CARS	chr11	3033424	-	ENST00000389048	ALK	chr2	29446394	-	4206	2065	246	3755	1169
ENST00000278224	CARS	chr11	3033424	-	ENST00000389048	ALK	chr2	29446394	-	4011	1870	51	3560	1169
ENST00000401769	CARS	chr11	3037010	-	ENST00000389048	ALK	chr2	29446394	-	4076	1935	159	2003	614
ENST00000397114	CARS	chr11	3037010	-	ENST00000389048	ALK	chr2	29446394	-	4225	2084	290	2152	620

DeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.

Henst

Tenst

Hgene

Hchr

Hbp

Hstrand

Tgene

Tchr

Tbp

Tstrand

No-coding score

Coding score

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Fusion Genomic Features for CARS-ALK

FusionAI prediction of the potential fusion gene breakpoint based on the pre-mature RNA sequence context (+/- 5kb of individual partner genes, total 20kb length sequence). FusionAI is a fusion gene breakpoint classifier based on convolutional neural network by comparing the fusion positive and negative sequence context of ~ 20K fusion gene data. From here, we can have the relative potentency of the 20K genomic sequence how individual sequnce will be likely used as the gene fusion breakpoints.

Hgene

Hchr

Hbp

Hstrand

Tgene

Tchr

Tbp

Tstrand

1-p

p (fusion gene breakpoint)

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Fusion Protein Features for CARS-ALK

Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chr11:/chr2:)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
4. Go down and press 'Search' tab twice.
4. Go down to have the hyperlink of the search result.
5. Click the hyperlink.
6. See the FGviewer result for your fusion gene.

Main function of each fusion partner protein. (from UniProt)

Hgene	Tgene
.	ALK Q9UM73
FUNCTION: Transcriptional activator which is required for calcium-dependent dendritic growth and branching in cortical neurons. Recruits CREB-binding protein (CREBBP) to nuclear bodies. Component of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating a calcium-dependent release of a repressor complex and a recruitment of an activator complex. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex. At the same time, there is increased recruitment of CREBBP to the promoter by a CREST-dependent mechanism, which leads to transcriptional activation. The CREST-BRG1 complex also binds to the NR2B promoter, and activity-dependent induction of NR2B expression involves a release of HDAC1 and recruitment of CREBBP (By similarity). {ECO:0000250}.	FUNCTION: Neuronal receptor tyrosine kinase that is essentially and transiently expressed in specific regions of the central and peripheral nervous systems and plays an important role in the genesis and differentiation of the nervous system. Transduces signals from ligands at the cell surface, through specific activation of the mitogen-activated protein kinase (MAPK) pathway. Phosphorylates almost exclusively at the first tyrosine of the Y-x-x-x-Y-Y motif. Following activation by ligand, ALK induces tyrosine phosphorylation of CBL, FRS2, IRS1 and SHC1, as well as of the MAP kinases MAPK1/ERK2 and MAPK3/ERK1. Acts as a receptor for ligands pleiotrophin (PTN), a secreted growth factor, and midkine (MDK), a PTN-related factor, thus participating in PTN and MDK signal transduction. PTN-binding induces MAPK pathway activation, which is important for the anti-apoptotic signaling of PTN and regulation of cell proliferation. MDK-binding induces phosphorylation of the ALK target insulin receptor substrate (IRS1), activates mitogen-activated protein kinases (MAPKs) and PI3-kinase, resulting also in cell proliferation induction. Drives NF-kappa-B activation, probably through IRS1 and the activation of the AKT serine/threonine kinase. Recruitment of IRS1 to activated ALK and the activation of NF-kappa-B are essential for the autocrine growth and survival signaling of MDK. Thinness gene involved in the resistance to weight gain: in hypothalamic neurons, controls energy expenditure acting as a negative regulator of white adipose tissue lipolysis and sympathetic tone to fine-tune energy homeostasis (By similarity). {ECO:0000250\|UniProtKB:P97793, ECO:0000269\|PubMed:11121404, ECO:0000269\|PubMed:11278720, ECO:0000269\|PubMed:11387242, ECO:0000269\|PubMed:11809760, ECO:0000269\|PubMed:12107166, ECO:0000269\|PubMed:12122009, ECO:0000269\|PubMed:15226403, ECO:0000269\|PubMed:15908427, ECO:0000269\|PubMed:16317043, ECO:0000269\|PubMed:16878150, ECO:0000269\|PubMed:17274988}.

Retention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at
download page

* Minus value of BPloci means that the break pointn is located before the CDS.

- In-frame and retained protein feature among the 13 regional features.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Protein feature

Protein feature note

- In-frame and not-retained protein feature among the 13 regional features.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Protein feature

Protein feature note

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Fusion Gene Sequence for CARS-ALK

For in-frame fusion transcripts, we provide the fusion transcript sequences and fusion amino acid sequences. To have fusion amino acid sequence, we ran ORFfinder and chose the longest ORF among the all predicted ones.

>13092_13092_1_CARS-ALK_CARS_chr11_3033424_ENST00000278224_ALK_chr2_29446394_ENST00000389048_length(transcript)=4011nt_BP=1870nt
AGATTCTAGGAAGTGTCTGTAGCCGCAGCTGCGGGTCCGGGATTCCCAGCCATGGCAGATTCCTCCGGGCAGCAGGGCAAAGGCCGGCGT
GTGCAGCCCCAGTGGTCCCCTCCTGCTGGGACCCAGCCATGCAGACTCCACCTTTACAACAGCCTCACCAGGAACAAGGAAGTGTTCATA
CCTCAAGATGGGAAAAAGGTGACGTGGTATTGCTGTGGGCCAACCGTCTATGACGCATCTCACATGGGGCACGCCAGGTCCTACATCTCT
TTTGATATCTTGAGAAGAGTGTTGAAGGATTACTTCAAATTTGATGTCTTTTATTGCATGAACATTACGGATATTGATGACAAGATCATC
AAGAGGGCCCGGCAGAACCACCTGTTCGAGCAGTATCGGGAGAAGAGGCCTGAAGCGGCACAGCTCTTGGAGGATGTTCAGGCCGCCCTG
AAGCCATTTTCAGTAAAATTAAATGAGACCACGGATCCCGATAAAAAGCAGATGCTCGAACGGATTCAGCACGCAGTGCAGCTTGCCACA
GAGCCACTTGAGAAAGCTGTGCAGTCCAGACTCACGGGAGAGGAAGTCAACAGCTGTGTGGAGGTGTTGCTGGAAGAAGCCAAGGATTTG
CTCTCTGACTGGCTGGATTCTACACTTGGCTGTGATGTCACTGACAATTCCATCTTCTCCAAGCTGCCCAAGTTCTGGGAGGGGGACTTC
CACAGAGACATGGAAGCTCTGAATGTTCTCCCTCCAGATGTCTTAACCCGGGTTAGTGAGTATGTGCCAGAAATTGTGAACTTTGTCCAG
AAGATTGTGGACAACGGTTACGGCTATGTCTCCAATGGGTCTGTCTACTTTGATACAGCGAAGTTTGCTTCTAGCGAGAAGCACTCCTAT
GGGAAGCTGGTGCCTGAGGCCGTTGGAGATCAGAAAGCCCTTCAAGAAGGGGAAGGTGACCTGAGCATCTCTGCAGACCGCCTGAGTGAG
AAGCGCTCTCCCAACGACTTTGCCTTATGGAAGGCCTCTAAGCCCGGAGAACCGTCCTGGCCGTGCCCTTGGGGAAAGGGTCGTCCGGGC
TGGCATATCGAGTGCTCGGCCATGGCAGGCACCCTCCTAGGGGCTTCGATGGACATTCACGGAGGTGGGTTCGACCTCCGGTTCCCCCAC
CATGACAATGAGCTGGCACAGTCGGAGGCCTACTTTGAAAACGACTGCTGGGTCAGGTACTTCCTGCACACAGGCCACCTGACCATTGCA
GGCTGCAAAATGTCAAAGTCACTAAAAAACTTCATCACCATTAAAGATGCCTTGAAAAAGCACTCAGCACGGCAGTTGCGGCTGGCCTTC
CTCATGCACTCGTGGAAGGACACCCTGGACTACTCCAGCAACACCATGGAGTCAGCGCTTCAATATGAGAAGTTCTTGAATGAGTTTTTC
TTAAATGTGAAAGATATCCTTCGCGCTCCTGTTGACATCACTGGTCAGTTTGAGAAGTGGGGAGAAGAAGAAGCAGAACTGAATAAGAAC
TTTTATGACAAGAAGACAGCAATTCACAAAGCCCTCTGTGACAATGTTGACACCCGCACCGTCATGGAAGAGATGCGGGCCTTGGTCAGT
CAGTGCAACCTCTATATGGCAGCCCGGAAAGCCGTGAGGAAGAGGCCCAACCAGGCTCTGCTGGAGAACATCGCCCTGTACCTCACCCAT
ATGCTGAAGATCTTTGGGGCCGTAGAAGAGGACAGCTCCCTGGGATTCCCGGTCGGAGGGCCTGGAACCAGCCTCAGTCTCGAGGCCACA
GTCATGCCCTACCTTCAGGTGTTATCAGAATTCCGAGAAGGAGTGCGGAAGATTGCCCGAGAGCAAAAAGTGTACCGCCGGAAGCACCAG
GAGCTGCAAGCCATGCAGATGGAGCTGCAGAGCCCTGAGTACAAGCTGAGCAAGCTCCGCACCTCGACCATCATGACCGACTACAACCCC
AACTACTGCTTTGCTGGCAAGACCTCCTCCATCAGTGACCTGAAGGAGGTGCCGCGGAAAAACATCACCCTCATTCGGGGTCTGGGCCAT
GGCGCCTTTGGGGAGGTGTATGAAGGCCAGGTGTCCGGAATGCCCAACGACCCAAGCCCCCTGCAAGTGGCTGTGAAGACGCTGCCTGAA
GTGTGCTCTGAACAGGACGAACTGGATTTCCTCATGGAAGCCCTGATCATCAGCAAATTCAACCACCAGAACATTGTTCGCTGCATTGGG
GTGAGCCTGCAATCCCTGCCCCGGTTCATCCTGCTGGAGCTCATGGCGGGGGGAGACCTCAAGTCCTTCCTCCGAGAGACCCGCCCTCGC
CCGAGCCAGCCCTCCTCCCTGGCCATGCTGGACCTTCTGCACGTGGCTCGGGACATTGCCTGTGGCTGTCAGTATTTGGAGGAAAACCAC
TTCATCCACCGAGACATTGCTGCCAGAAACTGCCTCTTGACCTGTCCAGGCCCTGGAAGAGTGGCCAAGATTGGAGACTTCGGGATGGCC
CGAGACATCTACAGGGCGAGCTACTATAGAAAGGGAGGCTGTGCCATGCTGCCAGTTAAGTGGATGCCCCCAGAGGCCTTCATGGAAGGA
ATATTCACTTCTAAAACAGACACATGGTCCTTTGGAGTGCTGCTATGGGAAATCTTTTCTCTTGGATATATGCCATACCCCAGCAAAAGC
AACCAGGAAGTTCTGGAGTTTGTCACCAGTGGAGGCCGGATGGACCCACCCAAGAACTGCCCTGGGCCTGTATACCGGATAATGACTCAG
TGCTGGCAACATCAGCCTGAAGACAGGCCCAACTTTGCCATCATTTTGGAGAGGATTGAATACTGCACCCAGGACCCGGATGTAATCAAC
ACCGCTTTGCCGATAGAATATGGTCCACTTGTGGAAGAGGAAGAGAAAGTGCCTGTGAGGCCCAAGGACCCTGAGGGGGTTCCTCCTCTC
CTGGTCTCTCAACAGGCAAAACGGGAGGAGGAGCGCAGCCCAGCTGCCCCACCACCTCTGCCTACCACCTCCTCTGGCAAGGCTGCAAAG
AAACCCACAGCTGCAGAGATCTCTGTTCGAGTCCCTAGAGGGCCGGCCGTGGAAGGGGGACACGTGAATATGGCATTCTCTCAGTCCAAC
CCTCCTTCGGAGTTGCACAAGGTCCACGGATCCAGAAACAAGCCCACCAGCTTGTGGAACCCAACGTACGGCTCCTGGTTTACAGAGAAA
CCCACCAAAAAGAATAATCCTATAGCAAAGAAGGAGCCACACGACAGGGGTAACCTGGGGCTGGAGGGAAGCTGTACTGTCCCACCTAAC
GTTGCAACTGGGAGACTTCCGGGGGCCTCACTGCTCCTAGAGCCCTCTTCGCTGACTGCCAATATGAAGGAGGTACCTCTGTTCAGGCTA
CGTCACTTCCCTTGTGGGAATGTCAATTACGGCTACCAGCAACAGGGCTTGCCCTTAGAAGCCGCTACTGCCCCTGGAGCTGGTCATTAC
GAGGATACCATTCTGAAAAGCAAGAATAGCATGAACCAGCCTGGGCCCTGAGCTCGGTCGCACACTCACTTCTCTTCCTTGGGATCCCTA
AGACCGTGGAGGAGAGAGAGGCAATGGCTCCTTCACAAACCAGAGACCAAATGTCACGTTTTGTTTTGTGCCAACCTATTTTGAAGTACC
ACCAAAAAAGCTGTATTTTGAAAATGCTTTAGAAAGGTTTTGAGCATGGGTTCATCCTATTCTTTCGAAAGAAGAAAATATCATAAAAAT
GAGTGATAAATACAAGGCCCAGATGTGGTTGCATAAGGTTTTTATGCATGTTTGTTGTATACTTCCTTATGCTTCTTTCAAATTGTGTGT
GCTCTGCTTCAATGTAGTCAGAATTAGCTGCTTCTATGTTTCATAGTTGGGGTCATAGATGTTTCCTTGCCTTGTTGATGTGGACATGAG

>13092_13092_1_CARS-ALK_CARS_chr11_3033424_ENST00000278224_ALK_chr2_29446394_ENST00000389048_length(amino acids)=1169AA_BP=606
MADSSGQQGKGRRVQPQWSPPAGTQPCRLHLYNSLTRNKEVFIPQDGKKVTWYCCGPTVYDASHMGHARSYISFDILRRVLKDYFKFDVF
YCMNITDIDDKIIKRARQNHLFEQYREKRPEAAQLLEDVQAALKPFSVKLNETTDPDKKQMLERIQHAVQLATEPLEKAVQSRLTGEEVN
SCVEVLLEEAKDLLSDWLDSTLGCDVTDNSIFSKLPKFWEGDFHRDMEALNVLPPDVLTRVSEYVPEIVNFVQKIVDNGYGYVSNGSVYF
DTAKFASSEKHSYGKLVPEAVGDQKALQEGEGDLSISADRLSEKRSPNDFALWKASKPGEPSWPCPWGKGRPGWHIECSAMAGTLLGASM
DIHGGGFDLRFPHHDNELAQSEAYFENDCWVRYFLHTGHLTIAGCKMSKSLKNFITIKDALKKHSARQLRLAFLMHSWKDTLDYSSNTME
SALQYEKFLNEFFLNVKDILRAPVDITGQFEKWGEEEAELNKNFYDKKTAIHKALCDNVDTRTVMEEMRALVSQCNLYMAARKAVRKRPN
QALLENIALYLTHMLKIFGAVEEDSSLGFPVGGPGTSLSLEATVMPYLQVLSEFREGVRKIAREQKVYRRKHQELQAMQMELQSPEYKLS
KLRTSTIMTDYNPNYCFAGKTSSISDLKEVPRKNITLIRGLGHGAFGEVYEGQVSGMPNDPSPLQVAVKTLPEVCSEQDELDFLMEALII
SKFNHQNIVRCIGVSLQSLPRFILLELMAGGDLKSFLRETRPRPSQPSSLAMLDLLHVARDIACGCQYLEENHFIHRDIAARNCLLTCPG
PGRVAKIGDFGMARDIYRASYYRKGGCAMLPVKWMPPEAFMEGIFTSKTDTWSFGVLLWEIFSLGYMPYPSKSNQEVLEFVTSGGRMDPP
KNCPGPVYRIMTQCWQHQPEDRPNFAIILERIEYCTQDPDVINTALPIEYGPLVEEEEKVPVRPKDPEGVPPLLVSQQAKREEERSPAAP
PPLPTTSSGKAAKKPTAAEISVRVPRGPAVEGGHVNMAFSQSNPPSELHKVHGSRNKPTSLWNPTYGSWFTEKPTKKNNPIAKKEPHDRG

--------------------------------------------------------------
>13092_13092_2_CARS-ALK_CARS_chr11_3033424_ENST00000380525_ALK_chr2_29446394_ENST00000389048_length(transcript)=4279nt_BP=2138nt
CCGGGGCGGCGGTTGCATCAGATTCTAGGAAGTGTCTGTAGCCGCAGCTGCGGGTCCGGGATTCCCAGCCATGGCAGATTCCTCCGGGCA
GCAGGCTCCTGACTACAGGTCCATTCTGAGCATTAGTGACGAGGCAGCCAGGGCACAAGCCCTGAACGAGCACCTCAGCACGCGTAGCTA
TGTCCAGGGGTACTCACTGTCCCAGGCAGACGTGGACGCGTTCAGGCAGCTCTCGGCCCCGCCCGCTGACCCCCAGCTCTTCCACGTGGC
TCGGTGGTTCAGGCACATAGAAGCGCTCCTGGGTAGCCCCTGTGGCAAAGGCCAGCCCTGCAGGCTCCAAGCAAGCAAAGGCCGGCGTGT
GCAGCCCCAGTGGTCCCCTCCTGCTGGGACCCAGCCATGCAGACTCCACCTTTACAACAGCCTCACCAGGAACAAGGAAGTGTTCATACC
TCAAGATGGGAAAAAGGTGACGTGGTATTGCTGTGGGCCAACCGTCTATGACGCATCTCACATGGGGCACGCCAGGTCCTACATCTCTTT
TGATATCTTGAGAAGAGTGTTGAAGGATTACTTCAAATTTGATGTCTTTTATTGCATGAACATTACGGATATTGATGACAAGATCATCAA
GAGGGCCCGGCAGAACCACCTGTTCGAGCAGTATCGGGAGAAGAGGCCTGAAGCGGCACAGCTCTTGGAGGATGTTCAGGCCGCCCTGAA
GCCATTTTCAGTAAAATTAAATGAGACCACGGATCCCGATAAAAAGCAGATGCTCGAACGGATTCAGCACGCAGTGCAGCTTGCCACAGA
GCCACTTGAGAAAGCTGTGCAGTCCAGACTCACGGGAGAGGAAGTCAACAGCTGTGTGGAGGTGTTGCTGGAAGAAGCCAAGGATTTGCT
CTCTGACTGGCTGGATTCTACACTTGGCTGTGATGTCACTGACAATTCCATCTTCTCCAAGCTGCCCAAGTTCTGGGAGGGGGACTTCCA
CAGAGACATGGAAGCTCTGAATGTTCTCCCTCCAGATGTCTTAACCCGGGTTAGTGAGTATGTGCCAGAAATTGTGAACTTTGTCCAGAA
GATTGTGGACAACGGTTACGGCTATGTCTCCAATGGGTCTGTCTACTTTGATACAGCGAAGTTTGCTTCTAGCGAGAAGCACTCCTATGG
GAAGCTGGTGCCTGAGGCCGTTGGAGATCAGAAAGCCCTTCAAGAAGGGGAAGGTGACCTGAGCATCTCTGCAGACCGCCTGAGTGAGAA
GCGCTCTCCCAACGACTTTGCCTTATGGAAGGCCTCTAAGCCCGGAGAACCGTCCTGGCCGTGCCCTTGGGGAAAGGGTCGTCCGGGCTG
GCATATCGAGTGCTCGGCCATGGCAGGCACCCTCCTAGGGGCTTCGATGGACATTCACGGAGGTGGGTTCGACCTCCGGTTCCCCCACCA
TGACAATGAGCTGGCACAGTCGGAGGCCTACTTTGAAAACGACTGCTGGGTCAGGTACTTCCTGCACACAGGCCACCTGACCATTGCAGG
CTGCAAAATGTCAAAGTCACTAAAAAACTTCATCACCATTAAAGATGCCTTGAAAAAGCACTCAGCACGGCAGTTGCGGCTGGCCTTCCT
CATGCACTCGTGGAAGGACACCCTGGACTACTCCAGCAACACCATGGAGTCAGCGCTTCAATATGAGAAGTTCTTGAATGAGTTTTTCTT
AAATGTGAAAGATATCCTTCGCGCTCCTGTTGACATCACTGGTCAGTTTGAGAAGTGGGGAGAAGAAGAAGCAGAACTGAATAAGAACTT
TTATGACAAGAAGACAGCAATTCACAAAGCCCTCTGTGACAATGTTGACACCCGCACCGTCATGGAAGAGATGCGGGCCTTGGTCAGTCA
GTGCAACCTCTATATGGCAGCCCGGAAAGCCGTGAGGAAGAGGCCCAACCAGGCTCTGCTGGAGAACATCGCCCTGTACCTCACCCATAT
GCTGAAGATCTTTGGGGCCGTAGAAGAGGACAGCTCCCTGGGATTCCCGGTCGGAGGGCCTGGAACCAGCCTCAGTCTCGAGGCCACAGT
CATGCCCTACCTTCAGGTGTTATCAGAATTCCGAGAAGGAGTGCGGAAGATTGCCCGAGAGCAAAAAGTGTACCGCCGGAAGCACCAGGA
GCTGCAAGCCATGCAGATGGAGCTGCAGAGCCCTGAGTACAAGCTGAGCAAGCTCCGCACCTCGACCATCATGACCGACTACAACCCCAA
CTACTGCTTTGCTGGCAAGACCTCCTCCATCAGTGACCTGAAGGAGGTGCCGCGGAAAAACATCACCCTCATTCGGGGTCTGGGCCATGG
CGCCTTTGGGGAGGTGTATGAAGGCCAGGTGTCCGGAATGCCCAACGACCCAAGCCCCCTGCAAGTGGCTGTGAAGACGCTGCCTGAAGT
GTGCTCTGAACAGGACGAACTGGATTTCCTCATGGAAGCCCTGATCATCAGCAAATTCAACCACCAGAACATTGTTCGCTGCATTGGGGT
GAGCCTGCAATCCCTGCCCCGGTTCATCCTGCTGGAGCTCATGGCGGGGGGAGACCTCAAGTCCTTCCTCCGAGAGACCCGCCCTCGCCC
GAGCCAGCCCTCCTCCCTGGCCATGCTGGACCTTCTGCACGTGGCTCGGGACATTGCCTGTGGCTGTCAGTATTTGGAGGAAAACCACTT
CATCCACCGAGACATTGCTGCCAGAAACTGCCTCTTGACCTGTCCAGGCCCTGGAAGAGTGGCCAAGATTGGAGACTTCGGGATGGCCCG
AGACATCTACAGGGCGAGCTACTATAGAAAGGGAGGCTGTGCCATGCTGCCAGTTAAGTGGATGCCCCCAGAGGCCTTCATGGAAGGAAT
ATTCACTTCTAAAACAGACACATGGTCCTTTGGAGTGCTGCTATGGGAAATCTTTTCTCTTGGATATATGCCATACCCCAGCAAAAGCAA
CCAGGAAGTTCTGGAGTTTGTCACCAGTGGAGGCCGGATGGACCCACCCAAGAACTGCCCTGGGCCTGTATACCGGATAATGACTCAGTG
CTGGCAACATCAGCCTGAAGACAGGCCCAACTTTGCCATCATTTTGGAGAGGATTGAATACTGCACCCAGGACCCGGATGTAATCAACAC
CGCTTTGCCGATAGAATATGGTCCACTTGTGGAAGAGGAAGAGAAAGTGCCTGTGAGGCCCAAGGACCCTGAGGGGGTTCCTCCTCTCCT
GGTCTCTCAACAGGCAAAACGGGAGGAGGAGCGCAGCCCAGCTGCCCCACCACCTCTGCCTACCACCTCCTCTGGCAAGGCTGCAAAGAA
ACCCACAGCTGCAGAGATCTCTGTTCGAGTCCCTAGAGGGCCGGCCGTGGAAGGGGGACACGTGAATATGGCATTCTCTCAGTCCAACCC
TCCTTCGGAGTTGCACAAGGTCCACGGATCCAGAAACAAGCCCACCAGCTTGTGGAACCCAACGTACGGCTCCTGGTTTACAGAGAAACC
CACCAAAAAGAATAATCCTATAGCAAAGAAGGAGCCACACGACAGGGGTAACCTGGGGCTGGAGGGAAGCTGTACTGTCCCACCTAACGT
TGCAACTGGGAGACTTCCGGGGGCCTCACTGCTCCTAGAGCCCTCTTCGCTGACTGCCAATATGAAGGAGGTACCTCTGTTCAGGCTACG
TCACTTCCCTTGTGGGAATGTCAATTACGGCTACCAGCAACAGGGCTTGCCCTTAGAAGCCGCTACTGCCCCTGGAGCTGGTCATTACGA
GGATACCATTCTGAAAAGCAAGAATAGCATGAACCAGCCTGGGCCCTGAGCTCGGTCGCACACTCACTTCTCTTCCTTGGGATCCCTAAG
ACCGTGGAGGAGAGAGAGGCAATGGCTCCTTCACAAACCAGAGACCAAATGTCACGTTTTGTTTTGTGCCAACCTATTTTGAAGTACCAC
CAAAAAAGCTGTATTTTGAAAATGCTTTAGAAAGGTTTTGAGCATGGGTTCATCCTATTCTTTCGAAAGAAGAAAATATCATAAAAATGA
GTGATAAATACAAGGCCCAGATGTGGTTGCATAAGGTTTTTATGCATGTTTGTTGTATACTTCCTTATGCTTCTTTCAAATTGTGTGTGC
TCTGCTTCAATGTAGTCAGAATTAGCTGCTTCTATGTTTCATAGTTGGGGTCATAGATGTTTCCTTGCCTTGTTGATGTGGACATGAGCC

>13092_13092_2_CARS-ALK_CARS_chr11_3033424_ENST00000380525_ALK_chr2_29446394_ENST00000389048_length(amino acids)=1252AA_BP=689
MADSSGQQAPDYRSILSISDEAARAQALNEHLSTRSYVQGYSLSQADVDAFRQLSAPPADPQLFHVARWFRHIEALLGSPCGKGQPCRLQ
ASKGRRVQPQWSPPAGTQPCRLHLYNSLTRNKEVFIPQDGKKVTWYCCGPTVYDASHMGHARSYISFDILRRVLKDYFKFDVFYCMNITD
IDDKIIKRARQNHLFEQYREKRPEAAQLLEDVQAALKPFSVKLNETTDPDKKQMLERIQHAVQLATEPLEKAVQSRLTGEEVNSCVEVLL
EEAKDLLSDWLDSTLGCDVTDNSIFSKLPKFWEGDFHRDMEALNVLPPDVLTRVSEYVPEIVNFVQKIVDNGYGYVSNGSVYFDTAKFAS
SEKHSYGKLVPEAVGDQKALQEGEGDLSISADRLSEKRSPNDFALWKASKPGEPSWPCPWGKGRPGWHIECSAMAGTLLGASMDIHGGGF
DLRFPHHDNELAQSEAYFENDCWVRYFLHTGHLTIAGCKMSKSLKNFITIKDALKKHSARQLRLAFLMHSWKDTLDYSSNTMESALQYEK
FLNEFFLNVKDILRAPVDITGQFEKWGEEEAELNKNFYDKKTAIHKALCDNVDTRTVMEEMRALVSQCNLYMAARKAVRKRPNQALLENI
ALYLTHMLKIFGAVEEDSSLGFPVGGPGTSLSLEATVMPYLQVLSEFREGVRKIAREQKVYRRKHQELQAMQMELQSPEYKLSKLRTSTI
MTDYNPNYCFAGKTSSISDLKEVPRKNITLIRGLGHGAFGEVYEGQVSGMPNDPSPLQVAVKTLPEVCSEQDELDFLMEALIISKFNHQN
IVRCIGVSLQSLPRFILLELMAGGDLKSFLRETRPRPSQPSSLAMLDLLHVARDIACGCQYLEENHFIHRDIAARNCLLTCPGPGRVAKI
GDFGMARDIYRASYYRKGGCAMLPVKWMPPEAFMEGIFTSKTDTWSFGVLLWEIFSLGYMPYPSKSNQEVLEFVTSGGRMDPPKNCPGPV
YRIMTQCWQHQPEDRPNFAIILERIEYCTQDPDVINTALPIEYGPLVEEEEKVPVRPKDPEGVPPLLVSQQAKREEERSPAAPPPLPTTS
SGKAAKKPTAAEISVRVPRGPAVEGGHVNMAFSQSNPPSELHKVHGSRNKPTSLWNPTYGSWFTEKPTKKNNPIAKKEPHDRGNLGLEGS

--------------------------------------------------------------
>13092_13092_3_CARS-ALK_CARS_chr11_3033424_ENST00000397111_ALK_chr2_29446394_ENST00000389048_length(transcript)=4206nt_BP=2065nt
GACTACTCCCAGCACGGCCACTGAACGCTTGGCCGCGCGATGCTCCGGAGCGCGGGTCGCCATTGGGTTGAACTACAGCTCCCAGCATGC
TCCGGGCCGGCGACATCCGGAGCCCCTGGTAGTTGCCTAGCGACCGGGCGTGTCCCGGAAGACGGGCCCGGCGTGGGGCGCGACTTCCGG
GGCGGCGGTTGCATCAGATTCTAGGAAGTGTCTGTAGCCGCAGCTGCGGGTCCGGGATTCCCAGCCATGGCAGATTCCTCCGGGCAGCAG
GGCAAAGGCCGGCGTGTGCAGCCCCAGTGGTCCCCTCCTGCTGGGACCCAGCCATGCAGACTCCACCTTTACAACAGCCTCACCAGGAAC
AAGGAAGTGTTCATACCTCAAGATGGGAAAAAGGTGACGTGGTATTGCTGTGGGCCAACCGTCTATGACGCATCTCACATGGGGCACGCC
AGGTCCTACATCTCTTTTGATATCTTGAGAAGAGTGTTGAAGGATTACTTCAAATTTGATGTCTTTTATTGCATGAACATTACGGATATT
GATGACAAGATCATCAAGAGGGCCCGGCAGAACCACCTGTTCGAGCAGTATCGGGAGAAGAGGCCTGAAGCGGCACAGCTCTTGGAGGAT
GTTCAGGCCGCCCTGAAGCCATTTTCAGTAAAATTAAATGAGACCACGGATCCCGATAAAAAGCAGATGCTCGAACGGATTCAGCACGCA
GTGCAGCTTGCCACAGAGCCACTTGAGAAAGCTGTGCAGTCCAGACTCACGGGAGAGGAAGTCAACAGCTGTGTGGAGGTGTTGCTGGAA
GAAGCCAAGGATTTGCTCTCTGACTGGCTGGATTCTACACTTGGCTGTGATGTCACTGACAATTCCATCTTCTCCAAGCTGCCCAAGTTC
TGGGAGGGGGACTTCCACAGAGACATGGAAGCTCTGAATGTTCTCCCTCCAGATGTCTTAACCCGGGTTAGTGAGTATGTGCCAGAAATT
GTGAACTTTGTCCAGAAGATTGTGGACAACGGTTACGGCTATGTCTCCAATGGGTCTGTCTACTTTGATACAGCGAAGTTTGCTTCTAGC
GAGAAGCACTCCTATGGGAAGCTGGTGCCTGAGGCCGTTGGAGATCAGAAAGCCCTTCAAGAAGGGGAAGGTGACCTGAGCATCTCTGCA
GACCGCCTGAGTGAGAAGCGCTCTCCCAACGACTTTGCCTTATGGAAGGCCTCTAAGCCCGGAGAACCGTCCTGGCCGTGCCCTTGGGGA
AAGGGTCGTCCGGGCTGGCATATCGAGTGCTCGGCCATGGCAGGCACCCTCCTAGGGGCTTCGATGGACATTCACGGAGGTGGGTTCGAC
CTCCGGTTCCCCCACCATGACAATGAGCTGGCACAGTCGGAGGCCTACTTTGAAAACGACTGCTGGGTCAGGTACTTCCTGCACACAGGC
CACCTGACCATTGCAGGCTGCAAAATGTCAAAGTCACTAAAAAACTTCATCACCATTAAAGATGCCTTGAAAAAGCACTCAGCACGGCAG
TTGCGGCTGGCCTTCCTCATGCACTCGTGGAAGGACACCCTGGACTACTCCAGCAACACCATGGAGTCAGCGCTTCAATATGAGAAGTTC
TTGAATGAGTTTTTCTTAAATGTGAAAGATATCCTTCGCGCTCCTGTTGACATCACTGGTCAGTTTGAGAAGTGGGGAGAAGAAGAAGCA
GAACTGAATAAGAACTTTTATGACAAGAAGACAGCAATTCACAAAGCCCTCTGTGACAATGTTGACACCCGCACCGTCATGGAAGAGATG
CGGGCCTTGGTCAGTCAGTGCAACCTCTATATGGCAGCCCGGAAAGCCGTGAGGAAGAGGCCCAACCAGGCTCTGCTGGAGAACATCGCC
CTGTACCTCACCCATATGCTGAAGATCTTTGGGGCCGTAGAAGAGGACAGCTCCCTGGGATTCCCGGTCGGAGGGCCTGGAACCAGCCTC
AGTCTCGAGGCCACAGTCATGCCCTACCTTCAGGTGTTATCAGAATTCCGAGAAGGAGTGCGGAAGATTGCCCGAGAGCAAAAAGTGTAC
CGCCGGAAGCACCAGGAGCTGCAAGCCATGCAGATGGAGCTGCAGAGCCCTGAGTACAAGCTGAGCAAGCTCCGCACCTCGACCATCATG
ACCGACTACAACCCCAACTACTGCTTTGCTGGCAAGACCTCCTCCATCAGTGACCTGAAGGAGGTGCCGCGGAAAAACATCACCCTCATT
CGGGGTCTGGGCCATGGCGCCTTTGGGGAGGTGTATGAAGGCCAGGTGTCCGGAATGCCCAACGACCCAAGCCCCCTGCAAGTGGCTGTG
AAGACGCTGCCTGAAGTGTGCTCTGAACAGGACGAACTGGATTTCCTCATGGAAGCCCTGATCATCAGCAAATTCAACCACCAGAACATT
GTTCGCTGCATTGGGGTGAGCCTGCAATCCCTGCCCCGGTTCATCCTGCTGGAGCTCATGGCGGGGGGAGACCTCAAGTCCTTCCTCCGA
GAGACCCGCCCTCGCCCGAGCCAGCCCTCCTCCCTGGCCATGCTGGACCTTCTGCACGTGGCTCGGGACATTGCCTGTGGCTGTCAGTAT
TTGGAGGAAAACCACTTCATCCACCGAGACATTGCTGCCAGAAACTGCCTCTTGACCTGTCCAGGCCCTGGAAGAGTGGCCAAGATTGGA
GACTTCGGGATGGCCCGAGACATCTACAGGGCGAGCTACTATAGAAAGGGAGGCTGTGCCATGCTGCCAGTTAAGTGGATGCCCCCAGAG
GCCTTCATGGAAGGAATATTCACTTCTAAAACAGACACATGGTCCTTTGGAGTGCTGCTATGGGAAATCTTTTCTCTTGGATATATGCCA
TACCCCAGCAAAAGCAACCAGGAAGTTCTGGAGTTTGTCACCAGTGGAGGCCGGATGGACCCACCCAAGAACTGCCCTGGGCCTGTATAC
CGGATAATGACTCAGTGCTGGCAACATCAGCCTGAAGACAGGCCCAACTTTGCCATCATTTTGGAGAGGATTGAATACTGCACCCAGGAC
CCGGATGTAATCAACACCGCTTTGCCGATAGAATATGGTCCACTTGTGGAAGAGGAAGAGAAAGTGCCTGTGAGGCCCAAGGACCCTGAG
GGGGTTCCTCCTCTCCTGGTCTCTCAACAGGCAAAACGGGAGGAGGAGCGCAGCCCAGCTGCCCCACCACCTCTGCCTACCACCTCCTCT
GGCAAGGCTGCAAAGAAACCCACAGCTGCAGAGATCTCTGTTCGAGTCCCTAGAGGGCCGGCCGTGGAAGGGGGACACGTGAATATGGCA
TTCTCTCAGTCCAACCCTCCTTCGGAGTTGCACAAGGTCCACGGATCCAGAAACAAGCCCACCAGCTTGTGGAACCCAACGTACGGCTCC
TGGTTTACAGAGAAACCCACCAAAAAGAATAATCCTATAGCAAAGAAGGAGCCACACGACAGGGGTAACCTGGGGCTGGAGGGAAGCTGT
ACTGTCCCACCTAACGTTGCAACTGGGAGACTTCCGGGGGCCTCACTGCTCCTAGAGCCCTCTTCGCTGACTGCCAATATGAAGGAGGTA
CCTCTGTTCAGGCTACGTCACTTCCCTTGTGGGAATGTCAATTACGGCTACCAGCAACAGGGCTTGCCCTTAGAAGCCGCTACTGCCCCT
GGAGCTGGTCATTACGAGGATACCATTCTGAAAAGCAAGAATAGCATGAACCAGCCTGGGCCCTGAGCTCGGTCGCACACTCACTTCTCT
TCCTTGGGATCCCTAAGACCGTGGAGGAGAGAGAGGCAATGGCTCCTTCACAAACCAGAGACCAAATGTCACGTTTTGTTTTGTGCCAAC
CTATTTTGAAGTACCACCAAAAAAGCTGTATTTTGAAAATGCTTTAGAAAGGTTTTGAGCATGGGTTCATCCTATTCTTTCGAAAGAAGA
AAATATCATAAAAATGAGTGATAAATACAAGGCCCAGATGTGGTTGCATAAGGTTTTTATGCATGTTTGTTGTATACTTCCTTATGCTTC
TTTCAAATTGTGTGTGCTCTGCTTCAATGTAGTCAGAATTAGCTGCTTCTATGTTTCATAGTTGGGGTCATAGATGTTTCCTTGCCTTGT

>13092_13092_3_CARS-ALK_CARS_chr11_3033424_ENST00000397111_ALK_chr2_29446394_ENST00000389048_length(amino acids)=1169AA_BP=606
MADSSGQQGKGRRVQPQWSPPAGTQPCRLHLYNSLTRNKEVFIPQDGKKVTWYCCGPTVYDASHMGHARSYISFDILRRVLKDYFKFDVF
YCMNITDIDDKIIKRARQNHLFEQYREKRPEAAQLLEDVQAALKPFSVKLNETTDPDKKQMLERIQHAVQLATEPLEKAVQSRLTGEEVN
SCVEVLLEEAKDLLSDWLDSTLGCDVTDNSIFSKLPKFWEGDFHRDMEALNVLPPDVLTRVSEYVPEIVNFVQKIVDNGYGYVSNGSVYF
DTAKFASSEKHSYGKLVPEAVGDQKALQEGEGDLSISADRLSEKRSPNDFALWKASKPGEPSWPCPWGKGRPGWHIECSAMAGTLLGASM
DIHGGGFDLRFPHHDNELAQSEAYFENDCWVRYFLHTGHLTIAGCKMSKSLKNFITIKDALKKHSARQLRLAFLMHSWKDTLDYSSNTME
SALQYEKFLNEFFLNVKDILRAPVDITGQFEKWGEEEAELNKNFYDKKTAIHKALCDNVDTRTVMEEMRALVSQCNLYMAARKAVRKRPN
QALLENIALYLTHMLKIFGAVEEDSSLGFPVGGPGTSLSLEATVMPYLQVLSEFREGVRKIAREQKVYRRKHQELQAMQMELQSPEYKLS
KLRTSTIMTDYNPNYCFAGKTSSISDLKEVPRKNITLIRGLGHGAFGEVYEGQVSGMPNDPSPLQVAVKTLPEVCSEQDELDFLMEALII
SKFNHQNIVRCIGVSLQSLPRFILLELMAGGDLKSFLRETRPRPSQPSSLAMLDLLHVARDIACGCQYLEENHFIHRDIAARNCLLTCPG
PGRVAKIGDFGMARDIYRASYYRKGGCAMLPVKWMPPEAFMEGIFTSKTDTWSFGVLLWEIFSLGYMPYPSKSNQEVLEFVTSGGRMDPP
KNCPGPVYRIMTQCWQHQPEDRPNFAIILERIEYCTQDPDVINTALPIEYGPLVEEEEKVPVRPKDPEGVPPLLVSQQAKREEERSPAAP
PPLPTTSSGKAAKKPTAAEISVRVPRGPAVEGGHVNMAFSQSNPPSELHKVHGSRNKPTSLWNPTYGSWFTEKPTKKNNPIAKKEPHDRG

--------------------------------------------------------------
>13092_13092_4_CARS-ALK_CARS_chr11_3037010_ENST00000397114_ALK_chr2_29446394_ENST00000389048_length(transcript)=4225nt_BP=2084nt
AGATTCTAGGAAGTGTCTGTAGCCGCAGCTGCGGGTCCGGGATTCCCAGCCATGGCAGATTCCTCCGGGCAGCAGGCTCCTGACTACAGG
TCCATTCTGAGCATTAGTGACGAGGCAGCCAGGGCACAAGCCCTGAACGAGCACCTCAGCACGCGTAGCTATGTCCAGGGGTACTCACTG
TCCCAGGCAGACGTGGACGCGTTCAGGCAGCTCTCGGCCCCGCCCGCTGACCCCCAGCTCTTCCACGTGGCTCGGTGGTTCAGGCACATA
GAAGCGCTCCTGGGTAGCCCCTGTGGCAAAGGCCAGCCCTGCAGGCTCCAAGCAAGCAAAGGCCGGCGTGTGCAGCCCCAGTGGTCCCCT
CCTGCTGGGACCCAGCCATGCAGACTCCACCTTTACAACAGCCTCACCAGGAACAAGTGTTTCTTGCATTTCTTGTGATCGTCATTCCTT
CATTCCTAACAAAGGAAGTGTTCATACCTCAAGATGGGAAAAAGGTGACGTGGTATTGCTGTGGGCCAACCGTCTATGACGCATCTCACA
TGGGGCACGCCAGGTCCTACATCTCTTTTGATATCTTGAGAAGAGTGTTGAAGGATTACTTCAAATTTGATGTCTTTTATTGCATGAACA
TTACGGATATTGATGACAAGATCATCAAGAGGGCCCGGCAGAACCACCTGTTCGAGCAGTATCGGGAGAAGAGGCCTGAAGCGGCACAGC
TCTTGGAGGATGTTCAGGCCGCCCTGAAGCCATTTTCAGTAAAATTAAATGAGACCACGGATCCCGATAAAAAGCAGATGCTCGAACGGA
TTCAGCACGCAGTGCAGCTTGCCACAGAGCCACTTGAGAAAGCTGTGCAGTCCAGACTCACGGGAGAGGAAGTCAACAGCTGTGTGGAGG
TGTTGCTGGAAGAAGCCAAGGATTTGCTCTCTGACTGGCTGGATTCTACACTTGGCTGTGATGTCACTGACAATTCCATCTTCTCCAAGC
TGCCCAAGTTCTGGGAGGGGGACTTCCACAGAGACATGGAAGCTCTGAATGTTCTCCCTCCAGATGTCTTAACCCGGGTTAGTGAGTATG
TGCCAGAAATTGTGAACTTTGTCCAGAAGATTGTGGACAACGGTTACGGCTATGTCTCCAATGGGTCTGTCTACTTTGATACAGCGAAGT
TTGCTTCTAGCGAGAAGCACTCCTATGGGAAGCTGGTGCCTGAGGCCGTTGGAGATCAGAAAGCCCTTCAAGAAGGGGAAGGTGACCTGA
GCATCTCTGCAGACCGCCTGAGTGAGAAGCGCTCTCCCAACGACTTTGCCTTATGGAAGGCCTCTAAGCCCGGAGAACCGTCCTGGCCGT
GCCCTTGGGGAAAGGGTCGTCCGGGCTGGCATATCGAGTGCTCGGCCATGGCAGGCACCCTCCTAGGGGCTTCGATGGACATTCACGGAG
GTGGGTTCGACCTCCGGTTCCCCCACCATGACAATGAGCTGGCACAGTCGGAGGCCTACTTTGAAAACGACTGCTGGGTCAGGTACTTCC
TGCACACAGGCCACCTGACCATTGCAGGCTGCAAAATGTCAAAGTCACTAAAAAACTTCATCACCATTAAAGATGCCTTGAAAAAGCACT
CAGCACGGCAGTTGCGGCTGGCCTTCCTCATGCACTCGTGGAAGGACACCCTGGACTACTCCAGCAACACCATGGAGTCAGCGCTTCAAT
ATGAGAAGTTCTTGAATGAGTTTTTCTTAAATGTGAAAGATATCCTTCGCGCTCCTGTTGACATCACTGGTCAGTTTGAGAAGTGGGGAG
AAGAAGAAGCAGAACTGAATAAGAACTTTTATGACAAGAAGACAGCAATTCACAAAGCCCTCTGTGACAATGTTGACACCCGCACCGTCA
TGGAAGAGATGCGGGCCTTGGTCAGTCAGTGCAACCTCTATATGGCAGCCCGGAAAGCCGTGAGGAAGAGGCCCAACCAGGCTCTGCTGG
AGAACATCGCCCTGTACCTCACCCATATGCTGAAGATCTTTGGGGCCGTAGAAGAGGACAGCTCCCTGGGATTCCCGGTCGGAGGGCCTG
GAACCAGCCTCAGTTGTACCGCCGGAAGCACCAGGAGCTGCAAGCCATGCAGATGGAGCTGCAGAGCCCTGAGTACAAGCTGAGCAAGCT
CCGCACCTCGACCATCATGACCGACTACAACCCCAACTACTGCTTTGCTGGCAAGACCTCCTCCATCAGTGACCTGAAGGAGGTGCCGCG
GAAAAACATCACCCTCATTCGGGGTCTGGGCCATGGCGCCTTTGGGGAGGTGTATGAAGGCCAGGTGTCCGGAATGCCCAACGACCCAAG
CCCCCTGCAAGTGGCTGTGAAGACGCTGCCTGAAGTGTGCTCTGAACAGGACGAACTGGATTTCCTCATGGAAGCCCTGATCATCAGCAA
ATTCAACCACCAGAACATTGTTCGCTGCATTGGGGTGAGCCTGCAATCCCTGCCCCGGTTCATCCTGCTGGAGCTCATGGCGGGGGGAGA
CCTCAAGTCCTTCCTCCGAGAGACCCGCCCTCGCCCGAGCCAGCCCTCCTCCCTGGCCATGCTGGACCTTCTGCACGTGGCTCGGGACAT
TGCCTGTGGCTGTCAGTATTTGGAGGAAAACCACTTCATCCACCGAGACATTGCTGCCAGAAACTGCCTCTTGACCTGTCCAGGCCCTGG
AAGAGTGGCCAAGATTGGAGACTTCGGGATGGCCCGAGACATCTACAGGGCGAGCTACTATAGAAAGGGAGGCTGTGCCATGCTGCCAGT
TAAGTGGATGCCCCCAGAGGCCTTCATGGAAGGAATATTCACTTCTAAAACAGACACATGGTCCTTTGGAGTGCTGCTATGGGAAATCTT
TTCTCTTGGATATATGCCATACCCCAGCAAAAGCAACCAGGAAGTTCTGGAGTTTGTCACCAGTGGAGGCCGGATGGACCCACCCAAGAA
CTGCCCTGGGCCTGTATACCGGATAATGACTCAGTGCTGGCAACATCAGCCTGAAGACAGGCCCAACTTTGCCATCATTTTGGAGAGGAT
TGAATACTGCACCCAGGACCCGGATGTAATCAACACCGCTTTGCCGATAGAATATGGTCCACTTGTGGAAGAGGAAGAGAAAGTGCCTGT
GAGGCCCAAGGACCCTGAGGGGGTTCCTCCTCTCCTGGTCTCTCAACAGGCAAAACGGGAGGAGGAGCGCAGCCCAGCTGCCCCACCACC
TCTGCCTACCACCTCCTCTGGCAAGGCTGCAAAGAAACCCACAGCTGCAGAGATCTCTGTTCGAGTCCCTAGAGGGCCGGCCGTGGAAGG
GGGACACGTGAATATGGCATTCTCTCAGTCCAACCCTCCTTCGGAGTTGCACAAGGTCCACGGATCCAGAAACAAGCCCACCAGCTTGTG
GAACCCAACGTACGGCTCCTGGTTTACAGAGAAACCCACCAAAAAGAATAATCCTATAGCAAAGAAGGAGCCACACGACAGGGGTAACCT
GGGGCTGGAGGGAAGCTGTACTGTCCCACCTAACGTTGCAACTGGGAGACTTCCGGGGGCCTCACTGCTCCTAGAGCCCTCTTCGCTGAC
TGCCAATATGAAGGAGGTACCTCTGTTCAGGCTACGTCACTTCCCTTGTGGGAATGTCAATTACGGCTACCAGCAACAGGGCTTGCCCTT
AGAAGCCGCTACTGCCCCTGGAGCTGGTCATTACGAGGATACCATTCTGAAAAGCAAGAATAGCATGAACCAGCCTGGGCCCTGAGCTCG
GTCGCACACTCACTTCTCTTCCTTGGGATCCCTAAGACCGTGGAGGAGAGAGAGGCAATGGCTCCTTCACAAACCAGAGACCAAATGTCA
CGTTTTGTTTTGTGCCAACCTATTTTGAAGTACCACCAAAAAAGCTGTATTTTGAAAATGCTTTAGAAAGGTTTTGAGCATGGGTTCATC
CTATTCTTTCGAAAGAAGAAAATATCATAAAAATGAGTGATAAATACAAGGCCCAGATGTGGTTGCATAAGGTTTTTATGCATGTTTGTT
GTATACTTCCTTATGCTTCTTTCAAATTGTGTGTGCTCTGCTTCAATGTAGTCAGAATTAGCTGCTTCTATGTTTCATAGTTGGGGTCAT

>13092_13092_4_CARS-ALK_CARS_chr11_3037010_ENST00000397114_ALK_chr2_29446394_ENST00000389048_length(amino acids)=620AA_BP=592
MWQRPALQAPSKQRPACAAPVVPSCWDPAMQTPPLQQPHQEQVFLAFLVIVIPSFLTKEVFIPQDGKKVTWYCCGPTVYDASHMGHARSY
ISFDILRRVLKDYFKFDVFYCMNITDIDDKIIKRARQNHLFEQYREKRPEAAQLLEDVQAALKPFSVKLNETTDPDKKQMLERIQHAVQL
ATEPLEKAVQSRLTGEEVNSCVEVLLEEAKDLLSDWLDSTLGCDVTDNSIFSKLPKFWEGDFHRDMEALNVLPPDVLTRVSEYVPEIVNF
VQKIVDNGYGYVSNGSVYFDTAKFASSEKHSYGKLVPEAVGDQKALQEGEGDLSISADRLSEKRSPNDFALWKASKPGEPSWPCPWGKGR
PGWHIECSAMAGTLLGASMDIHGGGFDLRFPHHDNELAQSEAYFENDCWVRYFLHTGHLTIAGCKMSKSLKNFITIKDALKKHSARQLRL
AFLMHSWKDTLDYSSNTMESALQYEKFLNEFFLNVKDILRAPVDITGQFEKWGEEEAELNKNFYDKKTAIHKALCDNVDTRTVMEEMRAL

--------------------------------------------------------------
>13092_13092_5_CARS-ALK_CARS_chr11_3037010_ENST00000401769_ALK_chr2_29446394_ENST00000389048_length(transcript)=4076nt_BP=1935nt
CTGTAGCCGCAGCTGCGGGTCCGGGATTCCCAGCCATGGCAGATTCCTCCGGGCAGCAGGCTTTTCCTTTGCGGTCTTGAAGGTGCATAG
GCTTGGTGCCTGTCCAAGAGCCTGATTATTTCAGAAGGATGAGTTTCATTTCTGAGGTTTCTGGGGTAGATGGAGCCTACCTCAGGGCCT
TTCCGCACAGACTTGGGATACCACTTGGTCGGCTGCTCATCAGGCAAAGGCCGGCGTGTGCAGCCCCAGTGGTCCCCTCCTGCTGGGACC
CAGCCATGCAGACTCCACCTTTACAACAGCCTCACCAGGAACAAGGAAGTGTTCATACCTCAAGATGGGAAAAAGGTGACGTGGTATTGC
TGTGGGCCAACCGTCTATGACGCATCTCACATGGGGCACGCCAGGTCCTACATCTCTTTTGATATCTTGAGAAGAGTGTTGAAGGATTAC
TTCAAATTTGATGTCTTTTATTGCATGAACATTACGGATATTGATGACAAGATCATCAAGAGGGCCCGGCAGAACCACCTGTTCGAGCAG
TATCGGGAGAAGAGGCCTGAAGCGGCACAGCTCTTGGAGGATGTTCAGGCCGCCCTGAAGCCATTTTCAGTAAAATTAAATGAGACCACG
GATCCCGATAAAAAGCAGATGCTCGAACGGATTCAGCACGCAGTGCAGCTTGCCACAGAGCCACTTGAGAAAGCTGTGCAGTCCAGACTC
ACGGGAGAGGAAGTCAACAGCTGTGTGGAGGTGTTGCTGGAAGAAGCCAAGGATTTGCTCTCTGACTGGCTGGATTCTACACTTGGCTGT
GATGTCACTGACAATTCCATCTTCTCCAAGCTGCCCAAGTTCTGGGAGGGGGACTTCCACAGAGACATGGAAGCTCTGAATGTTCTCCCT
CCAGATGTCTTAACCCGGGTTAGTGAGTATGTGCCAGAAATTGTGAACTTTGTCCAGAAGATTGTGGACAACGGTTACGGCTATGTCTCC
AATGGGTCTGTCTACTTTGATACAGCGAAGTTTGCTTCTAGCGAGAAGCACTCCTATGGGAAGCTGGTGCCTGAGGCCGTTGGAGATCAG
AAAGCCCTTCAAGAAGGGGAAGGTGACCTGAGCATCTCTGCAGACCGCCTGAGTGAGAAGCGCTCTCCCAACGACTTTGCCTTATGGAAG
GCCTCTAAGCCCGGAGAACCGTCCTGGCCGTGCCCTTGGGGAAAGGGTCGTCCGGGCTGGCATATCGAGTGCTCGGCCATGGCAGGCACC
CTCCTAGGGGCTTCGATGGACATTCACGGAGGTGGGTTCGACCTCCGGTTCCCCCACCATGACAATGAGCTGGCACAGTCGGAGGCCTAC
TTTGAAAACGACTGCTGGGTCAGGTACTTCCTGCACACAGGCCACCTGACCATTGCAGGCTGCAAAATGTCAAAGTCACTAAAAAACTTC
ATCACCATTAAAGATGCCTTGAAAAAGCACTCAGCACGGCAGTTGCGGCTGGCCTTCCTCATGCACTCGTGGAAGGACACCCTGGACTAC
TCCAGCAACACCATGGAGTCAGCGCTTCAATATGAGAAGTTCTTGAATGAGTTTTTCTTAAATGTGAAAGATATCCTTCGCGCTCCTGTT
GACATCACTGGTCAGTTTGAGAAGTGGGGAGAAGAAGAAGCAGAACTGAATAAGAACTTTTATGACAAGAAGACAGCAATTCACAAAGCC
CTCTGTGACAATGTTGACACCCGCACCGTCATGGAAGAGATGCGGGCCTTGGTCAGTCAGTGCAACCTCTATATGGCAGCCCGGAAAGCC
GTGAGGAAGAGGCCCAACCAGGCTCTGCTGGAGAACATCGCCCTGTACCTCACCCATATGCTGAAGATCTTTGGGGCCGTAGAAGAGGAC
AGCTCCCTGGGATTCCCGGTCGGAGGGCCTGGAACCAGCCTCAGTTGTACCGCCGGAAGCACCAGGAGCTGCAAGCCATGCAGATGGAGC
TGCAGAGCCCTGAGTACAAGCTGAGCAAGCTCCGCACCTCGACCATCATGACCGACTACAACCCCAACTACTGCTTTGCTGGCAAGACCT
CCTCCATCAGTGACCTGAAGGAGGTGCCGCGGAAAAACATCACCCTCATTCGGGGTCTGGGCCATGGCGCCTTTGGGGAGGTGTATGAAG
GCCAGGTGTCCGGAATGCCCAACGACCCAAGCCCCCTGCAAGTGGCTGTGAAGACGCTGCCTGAAGTGTGCTCTGAACAGGACGAACTGG
ATTTCCTCATGGAAGCCCTGATCATCAGCAAATTCAACCACCAGAACATTGTTCGCTGCATTGGGGTGAGCCTGCAATCCCTGCCCCGGT
TCATCCTGCTGGAGCTCATGGCGGGGGGAGACCTCAAGTCCTTCCTCCGAGAGACCCGCCCTCGCCCGAGCCAGCCCTCCTCCCTGGCCA
TGCTGGACCTTCTGCACGTGGCTCGGGACATTGCCTGTGGCTGTCAGTATTTGGAGGAAAACCACTTCATCCACCGAGACATTGCTGCCA
GAAACTGCCTCTTGACCTGTCCAGGCCCTGGAAGAGTGGCCAAGATTGGAGACTTCGGGATGGCCCGAGACATCTACAGGGCGAGCTACT
ATAGAAAGGGAGGCTGTGCCATGCTGCCAGTTAAGTGGATGCCCCCAGAGGCCTTCATGGAAGGAATATTCACTTCTAAAACAGACACAT
GGTCCTTTGGAGTGCTGCTATGGGAAATCTTTTCTCTTGGATATATGCCATACCCCAGCAAAAGCAACCAGGAAGTTCTGGAGTTTGTCA
CCAGTGGAGGCCGGATGGACCCACCCAAGAACTGCCCTGGGCCTGTATACCGGATAATGACTCAGTGCTGGCAACATCAGCCTGAAGACA
GGCCCAACTTTGCCATCATTTTGGAGAGGATTGAATACTGCACCCAGGACCCGGATGTAATCAACACCGCTTTGCCGATAGAATATGGTC
CACTTGTGGAAGAGGAAGAGAAAGTGCCTGTGAGGCCCAAGGACCCTGAGGGGGTTCCTCCTCTCCTGGTCTCTCAACAGGCAAAACGGG
AGGAGGAGCGCAGCCCAGCTGCCCCACCACCTCTGCCTACCACCTCCTCTGGCAAGGCTGCAAAGAAACCCACAGCTGCAGAGATCTCTG
TTCGAGTCCCTAGAGGGCCGGCCGTGGAAGGGGGACACGTGAATATGGCATTCTCTCAGTCCAACCCTCCTTCGGAGTTGCACAAGGTCC
ACGGATCCAGAAACAAGCCCACCAGCTTGTGGAACCCAACGTACGGCTCCTGGTTTACAGAGAAACCCACCAAAAAGAATAATCCTATAG
CAAAGAAGGAGCCACACGACAGGGGTAACCTGGGGCTGGAGGGAAGCTGTACTGTCCCACCTAACGTTGCAACTGGGAGACTTCCGGGGG
CCTCACTGCTCCTAGAGCCCTCTTCGCTGACTGCCAATATGAAGGAGGTACCTCTGTTCAGGCTACGTCACTTCCCTTGTGGGAATGTCA
ATTACGGCTACCAGCAACAGGGCTTGCCCTTAGAAGCCGCTACTGCCCCTGGAGCTGGTCATTACGAGGATACCATTCTGAAAAGCAAGA
ATAGCATGAACCAGCCTGGGCCCTGAGCTCGGTCGCACACTCACTTCTCTTCCTTGGGATCCCTAAGACCGTGGAGGAGAGAGAGGCAAT
GGCTCCTTCACAAACCAGAGACCAAATGTCACGTTTTGTTTTGTGCCAACCTATTTTGAAGTACCACCAAAAAAGCTGTATTTTGAAAAT
GCTTTAGAAAGGTTTTGAGCATGGGTTCATCCTATTCTTTCGAAAGAAGAAAATATCATAAAAATGAGTGATAAATACAAGGCCCAGATG
TGGTTGCATAAGGTTTTTATGCATGTTTGTTGTATACTTCCTTATGCTTCTTTCAAATTGTGTGTGCTCTGCTTCAATGTAGTCAGAATT
AGCTGCTTCTATGTTTCATAGTTGGGGTCATAGATGTTTCCTTGCCTTGTTGATGTGGACATGAGCCATTTGAGGGGAGAGGGAACGGAA

>13092_13092_5_CARS-ALK_CARS_chr11_3037010_ENST00000401769_ALK_chr2_29446394_ENST00000389048_length(amino acids)=614AA_BP=586
MEPTSGPFRTDLGYHLVGCSSGKGRRVQPQWSPPAGTQPCRLHLYNSLTRNKEVFIPQDGKKVTWYCCGPTVYDASHMGHARSYISFDIL
RRVLKDYFKFDVFYCMNITDIDDKIIKRARQNHLFEQYREKRPEAAQLLEDVQAALKPFSVKLNETTDPDKKQMLERIQHAVQLATEPLE
KAVQSRLTGEEVNSCVEVLLEEAKDLLSDWLDSTLGCDVTDNSIFSKLPKFWEGDFHRDMEALNVLPPDVLTRVSEYVPEIVNFVQKIVD
NGYGYVSNGSVYFDTAKFASSEKHSYGKLVPEAVGDQKALQEGEGDLSISADRLSEKRSPNDFALWKASKPGEPSWPCPWGKGRPGWHIE
CSAMAGTLLGASMDIHGGGFDLRFPHHDNELAQSEAYFENDCWVRYFLHTGHLTIAGCKMSKSLKNFITIKDALKKHSARQLRLAFLMHS
WKDTLDYSSNTMESALQYEKFLNEFFLNVKDILRAPVDITGQFEKWGEEEAELNKNFYDKKTAIHKALCDNVDTRTVMEEMRALVSQCNL

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Fusion Gene PPI Analysis for CARS-ALK

Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in
ChiPPI page.

Protein-protein interactors with each fusion partner protein in wild-type (BIOGRID-3.4.160)

Hgene

Hgene's interactors

Tgene

Tgene's interactors

- Retained PPIs in in-frame fusion.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Still interaction with

- Lost PPIs in in-frame fusion.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Interaction lost with

- Retained PPIs, but lost function due to frame-shift fusion.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Interaction lost with

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Related Drugs for CARS-ALK

Drugs targeting genes involved in this fusion gene.
(DrugBank Version 5.1.8 2021-05-08)

Partner	Gene	UniProtAcc	DrugBank ID	Drug name	Drug activity	Drug type	Drug status
Tgene	ALK	Q9UM73	DB08865	Crizotinib	Inhibitor	Small molecule	Approved
Tgene	ALK	Q9UM73	DB09063	Ceritinib	Antagonist	Small molecule	Approved
Tgene	ALK	Q9UM73	DB11363	Alectinib	Inhibitor	Small molecule	Approved\|Investigational
Tgene	ALK	Q9UM73	DB12010	Fostamatinib	Inhibitor	Small molecule	Approved\|Investigational
Tgene	ALK	Q9UM73	DB12130	Lorlatinib	Inhibitor	Small molecule	Approved\|Investigational
Tgene	ALK	Q9UM73	DB12141	Gilteritinib	Inhibitor	Small molecule	Approved\|Investigational
Tgene	ALK	Q9UM73	DB12267	Brigatinib	Inhibitor	Small molecule	Approved\|Investigational

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Related Diseases for CARS-ALK

Diseases associated with fusion partners.
(DisGeNet 4.0)

Partner	Gene	Disease ID	Disease name	# pubmeds	Source
Tgene		C0007131	Non-Small Cell Lung Carcinoma	28	CGI;CTD_human
Tgene		C0027819	Neuroblastoma	13	CGI;CTD_human;ORPHANET
Tgene		C0152013	Adenocarcinoma of lung (disorder)	8	CGI;CTD_human
Tgene		C2751681	NEUROBLASTOMA, SUSCEPTIBILITY TO, 3	8	CLINGEN;UNIPROT
Tgene		C0206180	Ki-1+ Anaplastic Large Cell Lymphoma	6	CGI;CTD_human
Tgene		C0334121	Inflammatory Myofibroblastic Tumor	4	CGI;CTD_human;ORPHANET
Tgene		C0018199	Granuloma, Plasma Cell	3	CTD_human
Tgene		C0007621	Neoplastic Cell Transformation	2	CTD_human
Tgene		C0027627	Neoplasm Metastasis	2	CTD_human
Tgene		C0238463	Papillary thyroid carcinoma	2	ORPHANET
Tgene		C0001973	Alcoholic Intoxication, Chronic	1	PSYGENET
Tgene		C0006118	Brain Neoplasms	1	CGI;CTD_human
Tgene		C0006142	Malignant neoplasm of breast	1	CTD_human
Tgene		C0007134	Renal Cell Carcinoma	1	CTD_human
Tgene		C0011570	Mental Depression	1	PSYGENET
Tgene		C0011581	Depressive disorder	1	PSYGENET
Tgene		C0027643	Neoplasm Recurrence, Local	1	CTD_human
Tgene		C0036341	Schizophrenia	1	PSYGENET
Tgene		C0079744	Diffuse Large B-Cell Lymphoma	1	CTD_human
Tgene		C0085269	Plasma Cell Granuloma, Pulmonary	1	CTD_human
Tgene		C0153633	Malignant neoplasm of brain	1	CGI;CTD_human
Tgene		C0278601	Inflammatory Breast Carcinoma	1	CTD_human
Tgene		C0279702	Conventional (Clear Cell) Renal Cell Carcinoma	1	CTD_human
Tgene		C0496899	Benign neoplasm of brain, unspecified	1	CTD_human
Tgene		C0678222	Breast Carcinoma	1	CTD_human
Tgene		C0750974	Brain Tumor, Primary	1	CTD_human
Tgene		C0750977	Recurrent Brain Neoplasm	1	CTD_human
Tgene		C0750979	Primary malignant neoplasm of brain	1	CTD_human
Tgene		C1257931	Mammary Neoplasms, Human	1	CTD_human
Tgene		C1266042	Chromophobe Renal Cell Carcinoma	1	CTD_human
Tgene		C1266043	Sarcomatoid Renal Cell Carcinoma	1	CTD_human
Tgene		C1266044	Collecting Duct Carcinoma of the Kidney	1	CTD_human
Tgene		C1306837	Papillary Renal Cell Carcinoma	1	CTD_human
Tgene		C1332079	Anaplastic Large Cell Lymphoma, ALK-Positive	1	ORPHANET
Tgene		C1458155	Mammary Neoplasms	1	CTD_human
Tgene		C1527390	Neoplasms, Intracranial	1	CTD_human
Tgene		C2931189	Neural crest tumor	1	ORPHANET
Tgene		C3899155	hereditary neuroblastoma	1	GENOMICS_ENGLAND
Tgene		C4704874	Mammary Carcinoma, Human	1	CTD_human

Fusion Gene Studies in Kim Lab

Fusion gene:CARS-ALK (FusionGDB2 ID:HG833TG238)

Fusion Gene Summary for CARS-ALK

Fusion Gene ORF analysis for CARS-ALK

Fusion Genomic Features for CARS-ALK

Fusion Protein Features for CARS-ALK

Fusion Gene Sequence for CARS-ALK

Fusion Gene PPI Analysis for CARS-ALK

Related Drugs for CARS-ALK

Related Diseases for CARS-ALK

Fusion Gene Studies
in Kim Lab