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Center for Computational Systems Medicine
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Fusion Gene and Fusion Protein Summary

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Fusion Amino Acid Sequences (multiple BPs and multiple gene isoforms)

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Fusion Protein Breakpoint Sequences - (for the Screening of the FusionNeoAntigens)

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Potential FusionNeoAntigens in HLA I - (netMHCpan v4.1 + deepHLApan v1.1)

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Potential FusionNeoAntigens in HLA II - (netMHCIIpan v4.1)

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Fusion Breakpoint 14 AA Peptide Structure - (RoseTTAFold)

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Filtering FusionNeoAntigens Through Checking the Interaction with HLAs in 3D - (Glide)

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Vaccine Design for the FusionNeoAntigens (RNA/protein sequences)

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Potential target of CAR-T therapy development

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Information on the samples that have these potential fusion neoantigens

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Fusion Protein Targeting Drugs - (Manual Curation)

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Fusion Protein Related diseases - (Manual Curation)

Fusion Protein:ZNRF3-EWSR1

Fusion Gene and Fusion Protein Summary

check button Fusion gene summary
Fusion partner gene informationFusion gene name: ZNRF3-EWSR1
FusionPDB ID: 103166
FusionGDB2.0 ID: 103166
HgeneTgene
Gene symbol

ZNRF3

EWSR1

Gene ID

84133

2130

Gene namezinc and ring finger 3EWS RNA binding protein 1
SynonymsBK747E2.3|RNF203EWS|EWS-FLI1|bK984G1.4
Cytomap

22q12.1

22q12.2

Type of geneprotein-codingprotein-coding
DescriptionE3 ubiquitin-protein ligase ZNRF3CTA-292E10.6RING finger protein 203RING-type E3 ubiquitin transferase ZNRF3novel C3HC4 type Zinc finger (ring finger)zinc/RING finger protein 3RNA-binding protein EWSEWS RNA-binding protein variant 6Ewing sarcoma breakpoint region 1Ewings sarcoma EWS-Fli1 (type 1) oncogene
Modification date2020031320200329
UniProtAcc.

Q01844

Main function of 5'-partner protein: FUNCTION: Might normally function as a transcriptional repressor. EWS-fusion-proteins (EFPS) may play a role in the tumorigenic process. They may disturb gene expression by mimicking, or interfering with the normal function of CTD-POLII within the transcription initiation complex. They may also contribute to an aberrant activation of the fusion protein target genes.
Ensembl transtripts involved in fusion geneENST idsENST00000544604, ENST00000332811, 
ENST00000402174, ENST00000406323, 
ENST00000332035, ENST00000333395, 
ENST00000331029, ENST00000332050, 
ENST00000397938, ENST00000406548, 
ENST00000414183, 
Fusion gene scores for assessment (based on all fusion genes of FusionGDB 2.0)* DoF score26 X 11 X 18=514817 X 58 X 10=9860
# samples 3948
** MAII scorelog2(39/5148*10)=-3.72246602447109
possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs).
DoF>8 and MAII<0		log2(48/9860*10)=-4.36048133565676
possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs).
DoF>8 and MAII<0
Fusion gene context

PubMed: ZNRF3 [Title/Abstract] AND EWSR1 [Title/Abstract] AND fusion [Title/Abstract]

Fusion neoantigen context

PubMed: ZNRF3 [Title/Abstract] AND EWSR1 [Title/Abstract] AND neoantigen [Title/Abstract]

Most frequent breakpoint (based on all fusion genes of FusionGDB 2.0)ZNRF3(29383189)-EWSR1(29669730), # samples:2
Anticipated loss of major functional domain due to fusion event.ZNRF3-EWSR1 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF.
ZNRF3-EWSR1 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF.
ZNRF3-EWSR1 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF.
ZNRF3-EWSR1 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF.
ZNRF3-EWSR1 seems lost the major protein functional domain in Hgene partner, which is a CGC due to the frame-shifted ORF.
ZNRF3-EWSR1 seems lost the major protein functional domain in Hgene partner, which is a essential gene due to the frame-shifted ORF.
ZNRF3-EWSR1 seems lost the major protein functional domain in Tgene partner, which is a CGC due to the frame-shifted ORF.
ZNRF3-EWSR1 seems lost the major protein functional domain in Tgene partner, which is a essential gene due to the frame-shifted ORF.
* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

check button Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez
PartnerGeneGO IDGO termPubMed ID
HgeneZNRF3

GO:0016567

protein ubiquitination

22575959



check button Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chr22:29383189/chr22:29669730)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
4. Go down and press 'Search' tab twice.
4. Go down to have the hyperlink of the search result.
5. Click the hyperlink.
6. See the FGviewer result for your fusion gene.
FGviewer

check buttonRetention analysis results of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features, are available here.

check buttonFusion gene breakpoints across ZNRF3 (5'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.
all structure

check buttonFusion gene breakpoints across EWSR1 (3'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.
all structure


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Fusion Amino Acid Sequences


check buttonFusion information from ORFfinder translation from full-length transcript sequence from FusionPDB.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandSeq length
(transcript)		BP loci
(transcript)		Predicted start
(transcript)		Predicted stop
(transcript)		Seq length
(amino acids)
ENST00000544604ZNRF3chr2229280054+ENST00000332050EWSR1chr2229692229+164547512871429
ENST00000544604ZNRF3chr2229280054+ENST00000397938EWSR1chr2229692229+164647512871429
ENST00000544604ZNRF3chr2229280054+ENST00000406548EWSR1chr2229692229+145747512871429
ENST00000544604ZNRF3chr2229280054+ENST00000331029EWSR1chr2229692229+164647512871429
ENST00000544604ZNRF3chr2229280054+ENST00000414183EWSR1chr2229692229+14644751751281368
ENST00000544604ZNRF3chr2229383189+ENST00000332050EWSR1chr2229669730+26146011752250691
ENST00000544604ZNRF3chr2229383189+ENST00000397938EWSR1chr2229669730+28346011752469764
ENST00000544604ZNRF3chr2229383189+ENST00000406548EWSR1chr2229669730+26426011752466763
ENST00000544604ZNRF3chr2229383189+ENST00000331029EWSR1chr2229669730+27206011752355726
ENST00000544604ZNRF3chr2229383189+ENST00000414183EWSR1chr2229669730+26676011752484769
ENST00000544604ZNRF3chr2229383189+ENST00000333395EWSR1chr2229669730+17456011751563462
ENST00000544604ZNRF3chr2229383189+ENST00000332035EWSR1chr2229669730+24776011752301708
ENST00000332811ZNRF3chr2229383189+ENST00000332050EWSR1chr2229669730+2304291571940627
ENST00000332811ZNRF3chr2229383189+ENST00000397938EWSR1chr2229669730+2524291572159700
ENST00000332811ZNRF3chr2229383189+ENST00000406548EWSR1chr2229669730+2332291572156699
ENST00000332811ZNRF3chr2229383189+ENST00000331029EWSR1chr2229669730+2410291572045662
ENST00000332811ZNRF3chr2229383189+ENST00000414183EWSR1chr2229669730+2357291572174705
ENST00000332811ZNRF3chr2229383189+ENST00000333395EWSR1chr2229669730+1435291571253398
ENST00000332811ZNRF3chr2229383189+ENST00000332035EWSR1chr2229669730+2167291571991644
ENST00000402174ZNRF3chr2229383189+ENST00000332050EWSR1chr2229669730+2232219931868591
ENST00000402174ZNRF3chr2229383189+ENST00000397938EWSR1chr2229669730+2452219932087664
ENST00000402174ZNRF3chr2229383189+ENST00000406548EWSR1chr2229669730+2260219932084663
ENST00000402174ZNRF3chr2229383189+ENST00000331029EWSR1chr2229669730+2338219931973626
ENST00000402174ZNRF3chr2229383189+ENST00000414183EWSR1chr2229669730+2285219932102669
ENST00000402174ZNRF3chr2229383189+ENST00000333395EWSR1chr2229669730+1363219931181362
ENST00000402174ZNRF3chr2229383189+ENST00000332035EWSR1chr2229669730+2095219931919608
ENST00000406323ZNRF3chr2229383189+ENST00000332050EWSR1chr2229669730+2163150241799591
ENST00000406323ZNRF3chr2229383189+ENST00000397938EWSR1chr2229669730+2383150242018664
ENST00000406323ZNRF3chr2229383189+ENST00000406548EWSR1chr2229669730+2191150242015663
ENST00000406323ZNRF3chr2229383189+ENST00000331029EWSR1chr2229669730+2269150241904626
ENST00000406323ZNRF3chr2229383189+ENST00000414183EWSR1chr2229669730+2216150242033669
ENST00000406323ZNRF3chr2229383189+ENST00000333395EWSR1chr2229669730+1294150241112362
ENST00000406323ZNRF3chr2229383189+ENST00000332035EWSR1chr2229669730+2026150241850608
ENST00000544604ZNRF3chr2229383189+ENST00000332050EWSR1chr2229669727+26146011752250691
ENST00000544604ZNRF3chr2229383189+ENST00000397938EWSR1chr2229669727+28346011752469764
ENST00000544604ZNRF3chr2229383189+ENST00000406548EWSR1chr2229669727+26426011752466763
ENST00000544604ZNRF3chr2229383189+ENST00000331029EWSR1chr2229669727+27206011752355726
ENST00000544604ZNRF3chr2229383189+ENST00000414183EWSR1chr2229669727+26676011752484769
ENST00000544604ZNRF3chr2229383189+ENST00000333395EWSR1chr2229669727+17456011751563462
ENST00000544604ZNRF3chr2229383189+ENST00000332035EWSR1chr2229669727+24776011752301708
ENST00000332811ZNRF3chr2229383189+ENST00000332050EWSR1chr2229669727+2304291571940627
ENST00000332811ZNRF3chr2229383189+ENST00000397938EWSR1chr2229669727+2524291572159700
ENST00000332811ZNRF3chr2229383189+ENST00000406548EWSR1chr2229669727+2332291572156699
ENST00000332811ZNRF3chr2229383189+ENST00000331029EWSR1chr2229669727+2410291572045662
ENST00000332811ZNRF3chr2229383189+ENST00000414183EWSR1chr2229669727+2357291572174705
ENST00000332811ZNRF3chr2229383189+ENST00000333395EWSR1chr2229669727+1435291571253398
ENST00000332811ZNRF3chr2229383189+ENST00000332035EWSR1chr2229669727+2167291571991644
ENST00000402174ZNRF3chr2229383189+ENST00000332050EWSR1chr2229669727+2232219931868591
ENST00000402174ZNRF3chr2229383189+ENST00000397938EWSR1chr2229669727+2452219932087664
ENST00000402174ZNRF3chr2229383189+ENST00000406548EWSR1chr2229669727+2260219932084663
ENST00000402174ZNRF3chr2229383189+ENST00000331029EWSR1chr2229669727+2338219931973626
ENST00000402174ZNRF3chr2229383189+ENST00000414183EWSR1chr2229669727+2285219932102669
ENST00000402174ZNRF3chr2229383189+ENST00000333395EWSR1chr2229669727+1363219931181362
ENST00000402174ZNRF3chr2229383189+ENST00000332035EWSR1chr2229669727+2095219931919608
ENST00000406323ZNRF3chr2229383189+ENST00000332050EWSR1chr2229669727+2163150241799591
ENST00000406323ZNRF3chr2229383189+ENST00000397938EWSR1chr2229669727+2383150242018664
ENST00000406323ZNRF3chr2229383189+ENST00000406548EWSR1chr2229669727+2191150242015663
ENST00000406323ZNRF3chr2229383189+ENST00000331029EWSR1chr2229669727+2269150241904626
ENST00000406323ZNRF3chr2229383189+ENST00000414183EWSR1chr2229669727+2216150242033669
ENST00000406323ZNRF3chr2229383189+ENST00000333395EWSR1chr2229669727+1294150241112362
ENST00000406323ZNRF3chr2229383189+ENST00000332035EWSR1chr2229669727+2026150241850608

check buttonDeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandNo-coding scoreCoding score
ENST00000544604ENST00000332050ZNRF3chr2229280054+EWSR1chr2229692229+0.44290780.5570922
ENST00000544604ENST00000397938ZNRF3chr2229280054+EWSR1chr2229692229+0.442930970.55706906
ENST00000544604ENST00000406548ZNRF3chr2229280054+EWSR1chr2229692229+0.41909340.58090657
ENST00000544604ENST00000331029ZNRF3chr2229280054+EWSR1chr2229692229+0.442930970.55706906
ENST00000544604ENST00000414183ZNRF3chr2229280054+EWSR1chr2229692229+0.415033160.58496684
ENST00000544604ENST00000332050ZNRF3chr2229383189+EWSR1chr2229669730+0.0099544850.9900455
ENST00000544604ENST00000397938ZNRF3chr2229383189+EWSR1chr2229669730+0.0069485510.9930514
ENST00000544604ENST00000406548ZNRF3chr2229383189+EWSR1chr2229669730+0.0104434440.9895565
ENST00000544604ENST00000331029ZNRF3chr2229383189+EWSR1chr2229669730+0.0097333320.9902666
ENST00000544604ENST00000414183ZNRF3chr2229383189+EWSR1chr2229669730+0.0099015230.9900984
ENST00000544604ENST00000333395ZNRF3chr2229383189+EWSR1chr2229669730+0.0070723120.99292773
ENST00000544604ENST00000332035ZNRF3chr2229383189+EWSR1chr2229669730+0.0172567130.98274326
ENST00000332811ENST00000332050ZNRF3chr2229383189+EWSR1chr2229669730+0.004377560.99562246
ENST00000332811ENST00000397938ZNRF3chr2229383189+EWSR1chr2229669730+0.0039433840.9960567
ENST00000332811ENST00000406548ZNRF3chr2229383189+EWSR1chr2229669730+0.0069084140.9930916
ENST00000332811ENST00000331029ZNRF3chr2229383189+EWSR1chr2229669730+0.0039281510.9960718
ENST00000332811ENST00000414183ZNRF3chr2229383189+EWSR1chr2229669730+0.0042955010.9957045
ENST00000332811ENST00000333395ZNRF3chr2229383189+EWSR1chr2229669730+0.0031456380.99685436
ENST00000332811ENST00000332035ZNRF3chr2229383189+EWSR1chr2229669730+0.0055447140.9944553
ENST00000402174ENST00000332050ZNRF3chr2229383189+EWSR1chr2229669730+0.0049833620.99501663
ENST00000402174ENST00000397938ZNRF3chr2229383189+EWSR1chr2229669730+0.0049316750.9950683
ENST00000402174ENST00000406548ZNRF3chr2229383189+EWSR1chr2229669730+0.009220790.9907793
ENST00000402174ENST00000331029ZNRF3chr2229383189+EWSR1chr2229669730+0.0047161190.9952839
ENST00000402174ENST00000414183ZNRF3chr2229383189+EWSR1chr2229669730+0.0057717330.99422824
ENST00000402174ENST00000333395ZNRF3chr2229383189+EWSR1chr2229669730+0.0033526270.9966474
ENST00000402174ENST00000332035ZNRF3chr2229383189+EWSR1chr2229669730+0.0071764370.9928236
ENST00000406323ENST00000332050ZNRF3chr2229383189+EWSR1chr2229669730+0.0050816930.9949183
ENST00000406323ENST00000397938ZNRF3chr2229383189+EWSR1chr2229669730+0.0050490520.994951
ENST00000406323ENST00000406548ZNRF3chr2229383189+EWSR1chr2229669730+0.0094042920.9905957
ENST00000406323ENST00000331029ZNRF3chr2229383189+EWSR1chr2229669730+0.0049034260.9950965
ENST00000406323ENST00000414183ZNRF3chr2229383189+EWSR1chr2229669730+0.0058006680.9941994
ENST00000406323ENST00000333395ZNRF3chr2229383189+EWSR1chr2229669730+0.0035116610.99648833
ENST00000406323ENST00000332035ZNRF3chr2229383189+EWSR1chr2229669730+0.0072233470.99277663
ENST00000544604ENST00000332050ZNRF3chr2229383189+EWSR1chr2229669727+0.0099544850.9900455
ENST00000544604ENST00000397938ZNRF3chr2229383189+EWSR1chr2229669727+0.0069485510.9930514
ENST00000544604ENST00000406548ZNRF3chr2229383189+EWSR1chr2229669727+0.0104434440.9895565
ENST00000544604ENST00000331029ZNRF3chr2229383189+EWSR1chr2229669727+0.0097333320.9902666
ENST00000544604ENST00000414183ZNRF3chr2229383189+EWSR1chr2229669727+0.0099015230.9900984
ENST00000544604ENST00000333395ZNRF3chr2229383189+EWSR1chr2229669727+0.0070723120.99292773
ENST00000544604ENST00000332035ZNRF3chr2229383189+EWSR1chr2229669727+0.0172567130.98274326
ENST00000332811ENST00000332050ZNRF3chr2229383189+EWSR1chr2229669727+0.004377560.99562246
ENST00000332811ENST00000397938ZNRF3chr2229383189+EWSR1chr2229669727+0.0039433840.9960567
ENST00000332811ENST00000406548ZNRF3chr2229383189+EWSR1chr2229669727+0.0069084140.9930916
ENST00000332811ENST00000331029ZNRF3chr2229383189+EWSR1chr2229669727+0.0039281510.9960718
ENST00000332811ENST00000414183ZNRF3chr2229383189+EWSR1chr2229669727+0.0042955010.9957045
ENST00000332811ENST00000333395ZNRF3chr2229383189+EWSR1chr2229669727+0.0031456380.99685436
ENST00000332811ENST00000332035ZNRF3chr2229383189+EWSR1chr2229669727+0.0055447140.9944553
ENST00000402174ENST00000332050ZNRF3chr2229383189+EWSR1chr2229669727+0.0049833620.99501663
ENST00000402174ENST00000397938ZNRF3chr2229383189+EWSR1chr2229669727+0.0049316750.9950683
ENST00000402174ENST00000406548ZNRF3chr2229383189+EWSR1chr2229669727+0.009220790.9907793
ENST00000402174ENST00000331029ZNRF3chr2229383189+EWSR1chr2229669727+0.0047161190.9952839
ENST00000402174ENST00000414183ZNRF3chr2229383189+EWSR1chr2229669727+0.0057717330.99422824
ENST00000402174ENST00000333395ZNRF3chr2229383189+EWSR1chr2229669727+0.0033526270.9966474
ENST00000402174ENST00000332035ZNRF3chr2229383189+EWSR1chr2229669727+0.0071764370.9928236
ENST00000406323ENST00000332050ZNRF3chr2229383189+EWSR1chr2229669727+0.0050816930.9949183
ENST00000406323ENST00000397938ZNRF3chr2229383189+EWSR1chr2229669727+0.0050490520.994951
ENST00000406323ENST00000406548ZNRF3chr2229383189+EWSR1chr2229669727+0.0094042920.9905957
ENST00000406323ENST00000331029ZNRF3chr2229383189+EWSR1chr2229669727+0.0049034260.9950965
ENST00000406323ENST00000414183ZNRF3chr2229383189+EWSR1chr2229669727+0.0058006680.9941994
ENST00000406323ENST00000333395ZNRF3chr2229383189+EWSR1chr2229669727+0.0035116610.99648833
ENST00000406323ENST00000332035ZNRF3chr2229383189+EWSR1chr2229669727+0.0072233470.99277663

check button Predicted full-length fusion amino acid sequences. For individual full-length fusion transcript sequence from FusionPDB, we ran ORFfinder and chose the longest ORF among all the predicted ones.

Get the fusion protein sequences from here.

Fusion protein sequence information is available in the fasta format.
>FusionGDB ID_FusionGDB isoform ID_FGname_Hgene_Hchr_Hbp_Henst_Tgene_Tchr_Tbp_Tenst_length(fusion AA) seq_BP

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Fusion Protein Breakpoint Sequences for ZNRF3-EWSR1

check button +/-13 AA sequence from the breakpoints of the fusion protein sequences.
HgeneHchrHbpTgeneTchrTbpLength(fusion protein)BP in fusion proteinPeptide

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Potential FusionNeoAntigen Information of ZNRF3-EWSR1 in HLA I

check button Multiple sequence alignments of the potential FusionNeoAntigens per fusion breakpoints. If the MSA is empty, then it means that there were predicted fusion neoantigens in this fusion breakpoint, but those predicted fusion neoantigens were not across the breakpoint, which is not fusion-specific.

check button Potential FusionNeoAntigen Information
* We used NetMHCpan v4.1 (%rank<0.5) and deepHLApan v1.1 (immunogenic score>0.5)
Fusion geneHchrHbpTgeneTchrTbpHLA IFusionNeoAntigen peptideBinding scoreImmunogenic scoreNeoantigen start (at BP 13)Neoantigen end (at BP 13)

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Potential FusionNeoAntigen Information of ZNRF3-EWSR1 in HLA II

check button Multiple sequence alignments of the potential FusionNeoAntigens per fusion breakpoints. If the MSA is empty, then it means that there were predicted fusion neoantigens in this fusion breakpoint, but those predicted fusion neoantigens were not across the breakpoint, which is not fusion-specific.

check button Potential FusionNeoAntigen Information
* We used NetMHCIIpan v4.1 (%rank<0.5).
Fusion geneHchrHbpTgeneTchrTbpHLA IIFusionNeoAntigen peptideNeoantigen start (at BP 13)Neoantigen end (at BP 13)

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Fusion breakpoint peptide structures of ZNRF3-EWSR1

check button3D structures of the fusion breakpoint peptide of 14AA sequence that have potential fusion neoantigens
* The minimum length of the amino acid sequence in RoseTTAFold is 14AA. Here, we predicted the 14AA fusion protein breakpoint sequence not the fusion neoantigen peptide, which is shorter than 14 AA.

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Filtering FusionNeoAntigens Through Checking the Interaction with HLAs in 3D of ZNRF3-EWSR1

check buttonVirtual screening between 25 HLAs (from PDB) and FusionNeoAntigens
* We used Glide to predict the interaction between HLAs and neoantigens.
HLA allelePDB IDFile nameBPseqDocking scoreGlide score

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Vaccine Design for the FusionNeoAntigens of ZNRF3-EWSR1

check button mRNA and peptide sequences of FusionNeoAntigens that have potential interaction with HLA-Is.
Fusion geneHchrHbpTchrTbpStart in +/-13AAEnd in +/-13AAFusionNeoAntigen peptide sequenceFusionNeoAntigen RNA sequence

check button mRNA and peptide sequences of FusionNeoAntigens that have potential interaction with HLA-IIs.
Fusion geneHchrHbpTchrTbpStart in +/-13AAEnd in +/-13AAFusionNeoAntigen peptideFusionNEoAntigen RNA sequence

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Information of the samples that have these potential fusion neoantigens of ZNRF3-EWSR1

check button These samples were reported as having these fusion breakpoints. For individual breakpoints, we checked the open reading frames considering multiple gene isoforms and chose the in-frame fusion genes only. Then, we made fusion protein sequences and predicted the fusion neoantigens. These fusion-positive samples may have these potential fusion neoantigens.
Cancer typeFusion geneHchrHbpHenstTchrTbpTenstSample

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Potential target of CAR-T therapy development for ZNRF3-EWSR1

check button Predicted 3D structure. We used RoseTTAFold.

check buttonRetention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, to provide the retention of the transmembrane domain, we only show the protein feature retention information of those transmembrane features


* Minus value of BPloci means that the break point is located before the CDS.
- In-frame and retained 'Transmembrane'.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note

check button Subcellular localization prediction of the transmembrane domain retained fusion proteins
* We used DeepLoc 1.0. The order of the X-axis of the barplot is as follows: Entry_ID, Localization, Type, Nucleus, Cytoplasm, Extracellular, Mitochondrion, Cell_membrane, Endoplasmic_reticulum, Plastid, Golgi.apparatus, Lysosome.Vacuole, Peroxisome. Y-axis is the output score of DeepLoc. Clicking the image will open a new tab with a large image.
HgeneHchrHbpHenstTgeneTchrTbpTenstDeepLoc result

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Related Drugs to ZNRF3-EWSR1

check button Drugs used for this fusion-positive patient.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)
HgeneTgeneDrugSourcePMID

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Related Diseases to ZNRF3-EWSR1

check button Diseases that have this fusion gene.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)
HgeneTgeneDiseaseSourcePMID

check button Diseases associated with fusion partners.
(DisGeNet 4.0)
PartnerGeneDisease IDDisease name# pubmedsSource
TgeneEWSR1C0553580Ewings sarcoma3CTD_human;ORPHANET
TgeneEWSR1C0002736Amyotrophic Lateral Sclerosis1GENOMICS_ENGLAND
TgeneEWSR1C0033578Prostatic Neoplasms1CTD_human
TgeneEWSR1C0206651Clear Cell Sarcoma of Soft Tissue1ORPHANET
TgeneEWSR1C0206663Neuroectodermal Tumor, Primitive1CTD_human
TgeneEWSR1C0279980Extra-osseous Ewing's sarcoma1ORPHANET
TgeneEWSR1C0334584Spongioblastoma1CTD_human
TgeneEWSR1C0334596Medulloepithelioma1CTD_human
TgeneEWSR1C0376358Malignant neoplasm of prostate1CTD_human
TgeneEWSR1C0700367Ependymoblastoma1CTD_human
TgeneEWSR1C0751675Cerebral Primitive Neuroectodermal Tumor1CTD_human
TgeneEWSR1C1275278Extraskeletal Myxoid Chondrosarcoma1ORPHANET