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Fusion Protein:C5orf56-NSD1

Fusion Gene and Fusion Protein Summary

Fusion gene summary

Fusion partner gene information	Fusion gene name: C5orf56-NSD1
	FusionPDB ID: 11882
	FusionGDB2.0 ID: 11882
		Hgene	Tgene
	Gene symbol	C5orf56	NSD1
	Gene ID	441108	64324
	Gene name	IRF1 antisense RNA 1	nuclear receptor binding SET domain protein 1
	Synonyms	C5orf56	ARA267\|KMT3B\|SOTOS\|SOTOS1\|STO
	Cytomap	5q31.1	5q35.3
	Type of gene	ncRNA	protein-coding
	Description	uncharacterized protein C5orf56	histone-lysine N-methyltransferase, H3 lysine-36 and H4 lysine-20 specificH3-K36-HMTaseH4-K20-HMTaseNR-binding SET domain-containing proteinandrogen receptor coactivator 267 kDa proteinandrogen receptor-associated coregulator 267androgen receptor-as
	Modification date	20200313	20200321
	UniProtAcc	.	.
Ensembl transtripts involved in fusion gene	ENST ids	ENST00000337752, ENST00000378947, ENST00000378953, ENST00000407797, ENST00000464024,	ENST00000511258, ENST00000347982, ENST00000354179, ENST00000361032, ENST00000439151,
Fusion gene scores for assessment (based on all fusion genes of FusionGDB 2.0)	* DoF score	5 X 2 X 4=40	12 X 14 X 7=1176
	# samples	7	17
	** MAII score	log2(7/40*10)=0.807354922057604 effective Gene in Pan-Cancer Fusion Genes (eGinPCFGs). DoF>8 and MAII>0	log2(17/1176*10)=-2.79028140869866 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0
Fusion gene context	PubMed: C5orf56 [Title/Abstract] AND NSD1 [Title/Abstract] AND fusion [Title/Abstract]
Fusion neoantigen context	PubMed: C5orf56 [Title/Abstract] AND NSD1 [Title/Abstract] AND neoantigen [Title/Abstract]
Most frequent breakpoint (based on all fusion genes of FusionGDB 2.0)	C5orf56(131785381)-NSD1(176662822), # samples:1
Anticipated loss of major functional domain due to fusion event.	C5orf56-NSD1 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. C5orf56-NSD1 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. C5orf56-NSD1 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF. C5orf56-NSD1 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF.

* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez

Partner	Gene	GO ID	GO term	PubMed ID
Tgene	NSD1	GO:0045893	positive regulation of transcription, DNA-templated	11509567

Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chr5:131785381/chr5:176662822)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
4. Go down and press 'Search' tab twice.
4. Go down to have the hyperlink of the search result.
5. Click the hyperlink.
6. See the FGviewer result for your fusion gene.

Retention analysis results of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features, are available here.

Fusion gene breakpoints across C5orf56 (5'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

Fusion gene breakpoints across NSD1 (3'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

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Fusion Amino Acid Sequences

Fusion information from ORFfinder translation from full-length transcript sequence from FusionPDB.

Henst	Tenst	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	Seq length (transcript)	BP loci (transcript)	Predicted start (transcript)	Predicted stop (transcript)	Seq length (amino acids)
ENST00000378953	C5orf56	chr5	131785381	+	ENST00000354179	NSD1	chr5	176662822	+	9610	564	32	4858	1608
ENST00000378953	C5orf56	chr5	131785381	+	ENST00000439151	NSD1	chr5	176662822	+	9615	564	32	4858	1608
ENST00000378953	C5orf56	chr5	131785381	+	ENST00000347982	NSD1	chr5	176662822	+	5162	564	32	4858	1608
ENST00000378953	C5orf56	chr5	131785381	+	ENST00000361032	NSD1	chr5	176662822	+	9610	564	32	4858	1608
ENST00000337752	C5orf56	chr5	131785381	+	ENST00000354179	NSD1	chr5	176662822	+	9265	219	20	4513	1497
ENST00000337752	C5orf56	chr5	131785381	+	ENST00000439151	NSD1	chr5	176662822	+	9270	219	20	4513	1497
ENST00000337752	C5orf56	chr5	131785381	+	ENST00000347982	NSD1	chr5	176662822	+	4817	219	20	4513	1497
ENST00000337752	C5orf56	chr5	131785381	+	ENST00000361032	NSD1	chr5	176662822	+	9265	219	20	4513	1497
ENST00000378947	C5orf56	chr5	131785381	+	ENST00000354179	NSD1	chr5	176662822	+	9257	211	12	4505	1497
ENST00000378947	C5orf56	chr5	131785381	+	ENST00000439151	NSD1	chr5	176662822	+	9262	211	12	4505	1497
ENST00000378947	C5orf56	chr5	131785381	+	ENST00000347982	NSD1	chr5	176662822	+	4809	211	12	4505	1497
ENST00000378947	C5orf56	chr5	131785381	+	ENST00000361032	NSD1	chr5	176662822	+	9257	211	12	4505	1497
ENST00000407797	C5orf56	chr5	131785381	+	ENST00000354179	NSD1	chr5	176662822	+	9257	211	12	4505	1497
ENST00000407797	C5orf56	chr5	131785381	+	ENST00000439151	NSD1	chr5	176662822	+	9262	211	12	4505	1497
ENST00000407797	C5orf56	chr5	131785381	+	ENST00000347982	NSD1	chr5	176662822	+	4809	211	12	4505	1497
ENST00000407797	C5orf56	chr5	131785381	+	ENST00000361032	NSD1	chr5	176662822	+	9257	211	12	4505	1497

DeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.

Henst	Tenst	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	No-coding score	Coding score
ENST00000378953	ENST00000354179	C5orf56	chr5	131785381	+	NSD1	chr5	176662822	+	0.000407771	0.99959224
ENST00000378953	ENST00000439151	C5orf56	chr5	131785381	+	NSD1	chr5	176662822	+	0.000407674	0.99959236
ENST00000378953	ENST00000347982	C5orf56	chr5	131785381	+	NSD1	chr5	176662822	+	0.001041852	0.9989581
ENST00000378953	ENST00000361032	C5orf56	chr5	131785381	+	NSD1	chr5	176662822	+	0.000407771	0.99959224
ENST00000337752	ENST00000354179	C5orf56	chr5	131785381	+	NSD1	chr5	176662822	+	0.000261982	0.99973804
ENST00000337752	ENST00000439151	C5orf56	chr5	131785381	+	NSD1	chr5	176662822	+	0.00026195	0.99973804
ENST00000337752	ENST00000347982	C5orf56	chr5	131785381	+	NSD1	chr5	176662822	+	0.000695765	0.9993042
ENST00000337752	ENST00000361032	C5orf56	chr5	131785381	+	NSD1	chr5	176662822	+	0.000261982	0.99973804
ENST00000378947	ENST00000354179	C5orf56	chr5	131785381	+	NSD1	chr5	176662822	+	0.000256216	0.99974376
ENST00000378947	ENST00000439151	C5orf56	chr5	131785381	+	NSD1	chr5	176662822	+	0.000256187	0.99974376
ENST00000378947	ENST00000347982	C5orf56	chr5	131785381	+	NSD1	chr5	176662822	+	0.000667964	0.999332
ENST00000378947	ENST00000361032	C5orf56	chr5	131785381	+	NSD1	chr5	176662822	+	0.000256216	0.99974376
ENST00000407797	ENST00000354179	C5orf56	chr5	131785381	+	NSD1	chr5	176662822	+	0.000256216	0.99974376
ENST00000407797	ENST00000439151	C5orf56	chr5	131785381	+	NSD1	chr5	176662822	+	0.000256187	0.99974376
ENST00000407797	ENST00000347982	C5orf56	chr5	131785381	+	NSD1	chr5	176662822	+	0.000667964	0.999332
ENST00000407797	ENST00000361032	C5orf56	chr5	131785381	+	NSD1	chr5	176662822	+	0.000256216	0.99974376

Predicted full-length fusion amino acid sequences. For individual full-length fusion transcript sequence from FusionPDB, we ran ORFfinder and chose the longest ORF among all the predicted ones.

Get the fusion protein sequences from here.

Fusion protein sequence information is available in the fasta format.
>FusionGDB ID_FusionGDB isoform ID_FGname_Hgene_Hchr_Hbp_Henst_Tgene_Tchr_Tbp_Tenst_length(fusion AA) seq_BP

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Fusion Protein Breakpoint Sequences for C5orf56-NSD1

+/-13 AA sequence from the breakpoints of the fusion protein sequences.

Hgene	Hchr	Hbp	Tgene	Tchr	Tbp	Length(fusion protein)	BP in fusion protein	Peptide
C5orf56	chr5	131785381	NSD1	chr5	176662822	211	66	VPEFVAYWRKTHQAVRSEKKRLRKPS
C5orf56	chr5	131785381	NSD1	chr5	176662822	219	66	VPEFVAYWRKTHQAVRSEKKRLRKPS
C5orf56	chr5	131785381	NSD1	chr5	176662822	564	177	VPEFVAYWRKTHQAVRSEKKRLRKPS

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Potential FusionNeoAntigen Information of C5orf56-NSD1 in HLA I

Multiple sequence alignments of the potential FusionNeoAntigens per fusion breakpoints. If the MSA is empty, then it means that there were predicted fusion neoantigens in this fusion breakpoint, but those predicted fusion neoantigens were not across the breakpoint, which is not fusion-specific.

C5orf56-NSD1_131785381_176662822.msa

Potential FusionNeoAntigen Information
* We used NetMHCpan v4.1 (%rank<0.5) and deepHLApan v1.1 (immunogenic score>0.5)

Fusion gene	Hchr	Hbp	Tgene	Tchr	Tbp	HLA I	FusionNeoAntigen peptide	Binding score	Immunogenic score	Neoantigen start (at BP 13)	Neoantigen end (at BP 13)
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	HLA-A30:08	KTHQAVRSE	0.9441	0.7309	9	18
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	HLA-A30:08	KTHQAVRSEK	0.9988	0.753	9	19
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	HLA-A30:08	KTHQAVRSEKK	0.9991	0.7902	9	20
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	HLA-A31:02	AYWRKTHQAVR	0.9725	0.6576	5	16
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	HLA-A31:01	AYWRKTHQAVR	0.979	0.6074	5	16
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	HLA-A30:01	KTHQAVRSE	0.9458	0.8363	9	18
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	HLA-C14:02	YWRKTHQAV	0.0365	0.9517	6	15
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	HLA-C14:03	YWRKTHQAV	0.0365	0.9517	6	15
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	HLA-A30:01	KTHQAVRSEK	0.9988	0.8716	9	19
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	HLA-A30:01	KTHQAVRSEKK	0.9991	0.8844	9	20

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Potential FusionNeoAntigen Information of C5orf56-NSD1 in HLA II

C5orf56-NSD1_131785381_176662822.msa

Potential FusionNeoAntigen Information
* We used NetMHCIIpan v4.1 (%rank<0.5).

Fusion gene	Hchr	Hbp	Tgene	Tchr	Tbp	HLA II	FusionNeoAntigen peptide	Neoantigen start (at BP 13)	Neoantigen end (at BP 13)
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-0303	HQAVRSEKKRLRKPS	11	26
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-0303	THQAVRSEKKRLRKP	10	25
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1102	HQAVRSEKKRLRKPS	11	26
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1102	THQAVRSEKKRLRKP	10	25
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1102	PEFVAYWRKTHQAVR	1	16
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1102	VPEFVAYWRKTHQAV	0	15
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1102	KTHQAVRSEKKRLRK	9	24
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1103	PEFVAYWRKTHQAVR	1	16
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1103	VPEFVAYWRKTHQAV	0	15
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1103	HQAVRSEKKRLRKPS	11	26
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1103	THQAVRSEKKRLRKP	10	25
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1111	VPEFVAYWRKTHQAV	0	15
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1111	PEFVAYWRKTHQAVR	1	16
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1114	HQAVRSEKKRLRKPS	11	26
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1114	VPEFVAYWRKTHQAV	0	15
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1114	THQAVRSEKKRLRKP	10	25
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1114	PEFVAYWRKTHQAVR	1	16
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1116	HQAVRSEKKRLRKPS	11	26
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1116	THQAVRSEKKRLRKP	10	25
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1116	PEFVAYWRKTHQAVR	1	16
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1116	VPEFVAYWRKTHQAV	0	15
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1116	KTHQAVRSEKKRLRK	9	24
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1120	HQAVRSEKKRLRKPS	11	26
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1120	VPEFVAYWRKTHQAV	0	15
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1120	THQAVRSEKKRLRKP	10	25
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1120	PEFVAYWRKTHQAVR	1	16
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1121	HQAVRSEKKRLRKPS	11	26
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1121	THQAVRSEKKRLRKP	10	25
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1121	KTHQAVRSEKKRLRK	9	24
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1121	PEFVAYWRKTHQAVR	1	16
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1121	VPEFVAYWRKTHQAV	0	15
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1136	HQAVRSEKKRLRKPS	11	26
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1136	THQAVRSEKKRLRKP	10	25
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1136	KTHQAVRSEKKRLRK	9	24
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1136	PEFVAYWRKTHQAVR	1	16
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1141	PEFVAYWRKTHQAVR	1	16
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1141	VPEFVAYWRKTHQAV	0	15
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1148	HQAVRSEKKRLRKPS	11	26
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1148	THQAVRSEKKRLRKP	10	25
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1148	PEFVAYWRKTHQAVR	1	16
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1148	VPEFVAYWRKTHQAV	0	15
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1155	PEFVAYWRKTHQAVR	1	16
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1155	VPEFVAYWRKTHQAV	0	15
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1155	HQAVRSEKKRLRKPS	11	26
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1155	THQAVRSEKKRLRKP	10	25
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1159	HQAVRSEKKRLRKPS	11	26
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1159	PEFVAYWRKTHQAVR	1	16
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1159	THQAVRSEKKRLRKP	10	25
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1159	VPEFVAYWRKTHQAV	0	15
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1163	PEFVAYWRKTHQAVR	1	16
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1163	VPEFVAYWRKTHQAV	0	15
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1163	HQAVRSEKKRLRKPS	11	26
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1163	THQAVRSEKKRLRKP	10	25
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1165	HQAVRSEKKRLRKPS	11	26
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1165	THQAVRSEKKRLRKP	10	25
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1165	PEFVAYWRKTHQAVR	1	16
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1165	VPEFVAYWRKTHQAV	0	15
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1165	KTHQAVRSEKKRLRK	9	24
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1168	HQAVRSEKKRLRKPS	11	26
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1168	THQAVRSEKKRLRKP	10	25
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1168	VPEFVAYWRKTHQAV	0	15
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1168	PEFVAYWRKTHQAVR	1	16
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1169	VPEFVAYWRKTHQAV	0	15
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1170	HQAVRSEKKRLRKPS	11	26
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1170	PEFVAYWRKTHQAVR	1	16
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1170	THQAVRSEKKRLRKP	10	25
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1170	VPEFVAYWRKTHQAV	0	15
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1170	KTHQAVRSEKKRLRK	9	24
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1176	PEFVAYWRKTHQAVR	1	16
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1176	VPEFVAYWRKTHQAV	0	15
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1176	HQAVRSEKKRLRKPS	11	26
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1176	THQAVRSEKKRLRKP	10	25
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1182	VPEFVAYWRKTHQAV	0	15
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1182	PEFVAYWRKTHQAVR	1	16
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1185	PEFVAYWRKTHQAVR	1	16
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1185	VPEFVAYWRKTHQAV	0	15
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1185	HQAVRSEKKRLRKPS	11	26
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1185	THQAVRSEKKRLRKP	10	25
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1186	HQAVRSEKKRLRKPS	11	26
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1186	THQAVRSEKKRLRKP	10	25
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1186	PEFVAYWRKTHQAVR	1	16
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1186	VPEFVAYWRKTHQAV	0	15
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1186	KTHQAVRSEKKRLRK	9	24
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1301	HQAVRSEKKRLRKPS	11	26
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1301	THQAVRSEKKRLRKP	10	25
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1301	PEFVAYWRKTHQAVR	1	16
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1301	VPEFVAYWRKTHQAV	0	15
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1301	KTHQAVRSEKKRLRK	9	24
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1302	HQAVRSEKKRLRKPS	11	26
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1302	VPEFVAYWRKTHQAV	0	15
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1302	THQAVRSEKKRLRKP	10	25
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1302	PEFVAYWRKTHQAVR	1	16
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1304	PEFVAYWRKTHQAVR	1	16
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1304	HQAVRSEKKRLRKPS	11	26
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1304	VPEFVAYWRKTHQAV	0	15
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1304	THQAVRSEKKRLRKP	10	25
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1308	HQAVRSEKKRLRKPS	11	26
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1308	THQAVRSEKKRLRKP	10	25
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1308	PEFVAYWRKTHQAVR	1	16
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1308	VPEFVAYWRKTHQAV	0	15
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1309	PEFVAYWRKTHQAVR	1	16
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1309	VPEFVAYWRKTHQAV	0	15
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1315	HQAVRSEKKRLRKPS	11	26
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1315	THQAVRSEKKRLRKP	10	25
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1315	PEFVAYWRKTHQAVR	1	16
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1315	VPEFVAYWRKTHQAV	0	15
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1315	KTHQAVRSEKKRLRK	9	24
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1316	HQAVRSEKKRLRKPS	11	26
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1316	THQAVRSEKKRLRKP	10	25
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1316	PEFVAYWRKTHQAVR	1	16
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1316	VPEFVAYWRKTHQAV	0	15
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1316	KTHQAVRSEKKRLRK	9	24
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1317	HQAVRSEKKRLRKPS	11	26
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1317	PEFVAYWRKTHQAVR	1	16
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1317	THQAVRSEKKRLRKP	10	25
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1317	VPEFVAYWRKTHQAV	0	15
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1319	HQAVRSEKKRLRKPS	11	26
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1319	THQAVRSEKKRLRKP	10	25
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1319	PEFVAYWRKTHQAVR	1	16
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1319	VPEFVAYWRKTHQAV	0	15
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1319	KTHQAVRSEKKRLRK	9	24
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1320	HQAVRSEKKRLRKPS	11	26
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1320	THQAVRSEKKRLRKP	10	25
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1320	KTHQAVRSEKKRLRK	9	24
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1320	PEFVAYWRKTHQAVR	1	16
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1322	HQAVRSEKKRLRKPS	11	26
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1322	THQAVRSEKKRLRKP	10	25
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1322	PEFVAYWRKTHQAVR	1	16
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1322	VPEFVAYWRKTHQAV	0	15
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1322	KTHQAVRSEKKRLRK	9	24
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1323	HQAVRSEKKRLRKPS	11	26
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1323	VPEFVAYWRKTHQAV	0	15
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1323	THQAVRSEKKRLRKP	10	25
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1323	PEFVAYWRKTHQAVR	1	16
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1324	PEFVAYWRKTHQAVR	1	16
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1324	VPEFVAYWRKTHQAV	0	15
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1324	HQAVRSEKKRLRKPS	11	26
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1324	THQAVRSEKKRLRKP	10	25
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1327	HQAVRSEKKRLRKPS	11	26
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1327	THQAVRSEKKRLRKP	10	25
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1327	KTHQAVRSEKKRLRK	9	24
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1327	PEFVAYWRKTHQAVR	1	16
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1329	HQAVRSEKKRLRKPS	11	26
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1329	THQAVRSEKKRLRKP	10	25
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1329	VPEFVAYWRKTHQAV	0	15
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1329	PEFVAYWRKTHQAVR	1	16
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1334	HQAVRSEKKRLRKPS	11	26
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1334	VPEFVAYWRKTHQAV	0	15
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1334	THQAVRSEKKRLRKP	10	25
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1334	PEFVAYWRKTHQAVR	1	16
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1335	HQAVRSEKKRLRKPS	11	26
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1335	THQAVRSEKKRLRKP	10	25
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1335	PEFVAYWRKTHQAVR	1	16
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1335	VPEFVAYWRKTHQAV	0	15
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1335	KTHQAVRSEKKRLRK	9	24
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1336	VPEFVAYWRKTHQAV	0	15
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1336	PEFVAYWRKTHQAVR	1	16
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1336	HQAVRSEKKRLRKPS	11	26
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1339	HQAVRSEKKRLRKPS	11	26
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1339	VPEFVAYWRKTHQAV	0	15
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1339	THQAVRSEKKRLRKP	10	25
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1339	PEFVAYWRKTHQAVR	1	16
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1341	HQAVRSEKKRLRKPS	11	26
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1341	THQAVRSEKKRLRKP	10	25
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1343	HQAVRSEKKRLRKPS	11	26
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1343	THQAVRSEKKRLRKP	10	25
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1351	HQAVRSEKKRLRKPS	11	26
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1351	THQAVRSEKKRLRKP	10	25
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1351	PEFVAYWRKTHQAVR	1	16
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1351	VPEFVAYWRKTHQAV	0	15
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1351	KTHQAVRSEKKRLRK	9	24
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1352	HQAVRSEKKRLRKPS	11	26
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1352	THQAVRSEKKRLRKP	10	25
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1352	PEFVAYWRKTHQAVR	1	16
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1352	VPEFVAYWRKTHQAV	0	15
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1352	KTHQAVRSEKKRLRK	9	24
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1353	HQAVRSEKKRLRKPS	11	26
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1353	THQAVRSEKKRLRKP	10	25
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1353	PEFVAYWRKTHQAVR	1	16
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1353	VPEFVAYWRKTHQAV	0	15
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1353	KTHQAVRSEKKRLRK	9	24
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1357	HQAVRSEKKRLRKPS	11	26
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1357	THQAVRSEKKRLRKP	10	25
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1357	PEFVAYWRKTHQAVR	1	16
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1357	VPEFVAYWRKTHQAV	0	15
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1357	KTHQAVRSEKKRLRK	9	24
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1359	HQAVRSEKKRLRKPS	11	26
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1359	THQAVRSEKKRLRKP	10	25
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1359	PEFVAYWRKTHQAVR	1	16
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1359	VPEFVAYWRKTHQAV	0	15
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1359	KTHQAVRSEKKRLRK	9	24
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1361	HQAVRSEKKRLRKPS	11	26
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1361	THQAVRSEKKRLRKP	10	25
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1361	KTHQAVRSEKKRLRK	9	24
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1361	PEFVAYWRKTHQAVR	1	16
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1363	VPEFVAYWRKTHQAV	0	15
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1363	PEFVAYWRKTHQAVR	1	16
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1364	HQAVRSEKKRLRKPS	11	26
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1364	THQAVRSEKKRLRKP	10	25
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1364	PEFVAYWRKTHQAVR	1	16
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1364	VPEFVAYWRKTHQAV	0	15
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1364	KTHQAVRSEKKRLRK	9	24
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1368	HQAVRSEKKRLRKPS	11	26
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1368	THQAVRSEKKRLRKP	10	25
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1368	PEFVAYWRKTHQAVR	1	16
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1368	VPEFVAYWRKTHQAV	0	15
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1368	KTHQAVRSEKKRLRK	9	24
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1369	HQAVRSEKKRLRKPS	11	26
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1369	THQAVRSEKKRLRKP	10	25
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1369	PEFVAYWRKTHQAVR	1	16
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1369	VPEFVAYWRKTHQAV	0	15
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1369	KTHQAVRSEKKRLRK	9	24
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1370	HQAVRSEKKRLRKPS	11	26
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1370	THQAVRSEKKRLRKP	10	25
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1370	PEFVAYWRKTHQAVR	1	16
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1370	VPEFVAYWRKTHQAV	0	15
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1371	HQAVRSEKKRLRKPS	11	26
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1371	THQAVRSEKKRLRKP	10	25
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1371	KTHQAVRSEKKRLRK	9	24
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1372	HQAVRSEKKRLRKPS	11	26
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1372	THQAVRSEKKRLRKP	10	25
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1372	PEFVAYWRKTHQAVR	1	16
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1372	VPEFVAYWRKTHQAV	0	15
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1373	HQAVRSEKKRLRKPS	11	26
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1373	VPEFVAYWRKTHQAV	0	15
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1373	THQAVRSEKKRLRKP	10	25
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1373	PEFVAYWRKTHQAVR	1	16
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1374	HQAVRSEKKRLRKPS	11	26
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1374	VPEFVAYWRKTHQAV	0	15
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1374	THQAVRSEKKRLRKP	10	25
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1374	PEFVAYWRKTHQAVR	1	16
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1375	PEFVAYWRKTHQAVR	1	16
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1375	VPEFVAYWRKTHQAV	0	15
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1376	HQAVRSEKKRLRKPS	11	26
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1376	THQAVRSEKKRLRKP	10	25
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1376	PEFVAYWRKTHQAVR	1	16
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1378	HQAVRSEKKRLRKPS	11	26
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1378	THQAVRSEKKRLRKP	10	25
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1378	KTHQAVRSEKKRLRK	9	24
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1378	PEFVAYWRKTHQAVR	1	16
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1379	HQAVRSEKKRLRKPS	11	26
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1379	THQAVRSEKKRLRKP	10	25
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1379	PEFVAYWRKTHQAVR	1	16
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1379	VPEFVAYWRKTHQAV	0	15
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1379	KTHQAVRSEKKRLRK	9	24
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1380	HQAVRSEKKRLRKPS	11	26
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1380	THQAVRSEKKRLRKP	10	25
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1380	PEFVAYWRKTHQAVR	1	16
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1380	VPEFVAYWRKTHQAV	0	15
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1380	KTHQAVRSEKKRLRK	9	24
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1383	HQAVRSEKKRLRKPS	11	26
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1383	THQAVRSEKKRLRKP	10	25
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1383	PEFVAYWRKTHQAVR	1	16
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1383	VPEFVAYWRKTHQAV	0	15
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1383	KTHQAVRSEKKRLRK	9	24
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1384	HQAVRSEKKRLRKPS	11	26
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1384	THQAVRSEKKRLRKP	10	25
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1384	PEFVAYWRKTHQAVR	1	16
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1384	VPEFVAYWRKTHQAV	0	15
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1387	HQAVRSEKKRLRKPS	11	26
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1387	THQAVRSEKKRLRKP	10	25
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1387	PEFVAYWRKTHQAVR	1	16
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1387	VPEFVAYWRKTHQAV	0	15
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1387	KTHQAVRSEKKRLRK	9	24
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1391	HQAVRSEKKRLRKPS	11	26
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1391	THQAVRSEKKRLRKP	10	25
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1391	PEFVAYWRKTHQAVR	1	16
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1391	VPEFVAYWRKTHQAV	0	15
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1391	KTHQAVRSEKKRLRK	9	24
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1392	HQAVRSEKKRLRKPS	11	26
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1392	THQAVRSEKKRLRKP	10	25
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1392	PEFVAYWRKTHQAVR	1	16
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1392	VPEFVAYWRKTHQAV	0	15
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1392	KTHQAVRSEKKRLRK	9	24
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1393	PEFVAYWRKTHQAVR	1	16
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1393	HQAVRSEKKRLRKPS	11	26
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1393	VPEFVAYWRKTHQAV	0	15
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1393	THQAVRSEKKRLRKP	10	25
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1396	HQAVRSEKKRLRKPS	11	26
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1396	THQAVRSEKKRLRKP	10	25
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1396	KTHQAVRSEKKRLRK	9	24
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1397	HQAVRSEKKRLRKPS	11	26
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1397	VPEFVAYWRKTHQAV	0	15
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1397	THQAVRSEKKRLRKP	10	25
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1397	PEFVAYWRKTHQAVR	1	16
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1398	HQAVRSEKKRLRKPS	11	26
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1398	THQAVRSEKKRLRKP	10	25
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1398	PEFVAYWRKTHQAVR	1	16
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1398	VPEFVAYWRKTHQAV	0	15
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1398	KTHQAVRSEKKRLRK	9	24
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1399	HQAVRSEKKRLRKPS	11	26
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1399	VPEFVAYWRKTHQAV	0	15
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1399	THQAVRSEKKRLRKP	10	25
C5orf56-NSD1	chr5	131785381	chr5	176662822	219	DRB1-1399	PEFVAYWRKTHQAVR	1	16

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Fusion breakpoint peptide structures of C5orf56-NSD1

3D structures of the fusion breakpoint peptide of 14AA sequence that have potential fusion neoantigens
* The minimum length of the amino acid sequence in RoseTTAFold is 14AA. Here, we predicted the 14AA fusion protein breakpoint sequence not the fusion neoantigen peptide, which is shorter than 14 AA.

File name	BPseq	Hgene	Tgene	Hchr	Hbp	Tchr	Tbp	AAlen
10858	YWRKTHQAVRSEKK	C5orf56	NSD1	chr5	131785381	chr5	176662822	219

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Filtering FusionNeoAntigens Through Checking the Interaction with HLAs in 3D of C5orf56-NSD1

Virtual screening between 25 HLAs (from PDB) and FusionNeoAntigens
* We used Glide to predict the interaction between HLAs and neoantigens.

HLA allele	PDB ID	File name	BPseq	Docking score	Glide score
HLA-B14:02	3BVN	10858	YWRKTHQAVRSEKK	-7.9962	-8.1096
HLA-B14:02	3BVN	10858	YWRKTHQAVRSEKK	-5.70842	-6.74372
HLA-B52:01	3W39	10858	YWRKTHQAVRSEKK	-6.83737	-6.95077
HLA-B52:01	3W39	10858	YWRKTHQAVRSEKK	-4.4836	-5.5189
HLA-A11:01	4UQ2	10858	YWRKTHQAVRSEKK	-10.0067	-10.1201
HLA-A11:01	4UQ2	10858	YWRKTHQAVRSEKK	-9.03915	-10.0745
HLA-A24:02	5HGA	10858	YWRKTHQAVRSEKK	-6.56204	-6.67544
HLA-A24:02	5HGA	10858	YWRKTHQAVRSEKK	-5.42271	-6.45801
HLA-B44:05	3DX8	10858	YWRKTHQAVRSEKK	-7.85648	-8.89178
HLA-B44:05	3DX8	10858	YWRKTHQAVRSEKK	-5.3978	-5.5112
HLA-A02:01	6TDR	10858	YWRKTHQAVRSEKK	-3.37154	-4.40684

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Vaccine Design for the FusionNeoAntigens of C5orf56-NSD1

mRNA and peptide sequences of FusionNeoAntigens that have potential interaction with HLA-Is.

Fusion gene	Hchr	Hbp	Tchr	Tbp	Start in +/-13AA	End in +/-13AA	FusionNeoAntigen peptide sequence	FusionNeoAntigen RNA sequence
C5orf56-NSD1	chr5	131785381	chr5	176662822	5	16	AYWRKTHQAVR	CCTATTGGAGGAAAACACACCAAGCTGTGCGGT
C5orf56-NSD1	chr5	131785381	chr5	176662822	6	15	YWRKTHQAV	ATTGGAGGAAAACACACCAAGCTGTGC
C5orf56-NSD1	chr5	131785381	chr5	176662822	9	18	KTHQAVRSE	AAACACACCAAGCTGTGCGGTCAGAGA
C5orf56-NSD1	chr5	131785381	chr5	176662822	9	19	KTHQAVRSEK	AAACACACCAAGCTGTGCGGTCAGAGAAGA
C5orf56-NSD1	chr5	131785381	chr5	176662822	9	20	KTHQAVRSEKK	AAACACACCAAGCTGTGCGGTCAGAGAAGAAAC

mRNA and peptide sequences of FusionNeoAntigens that have potential interaction with HLA-IIs.

Fusion gene	Hchr	Hbp	Tchr	Tbp	Start in +/-13AA	End in +/-13AA	FusionNeoAntigen peptide	FusionNEoAntigen RNA sequence
C5orf56-NSD1	chr5	131785381	chr5	176662822	0	15	VPEFVAYWRKTHQAV	TGCCAGAGTTTGTAGCCTATTGGAGGAAAACACACCAAGCTGTGC
C5orf56-NSD1	chr5	131785381	chr5	176662822	1	16	PEFVAYWRKTHQAVR	CAGAGTTTGTAGCCTATTGGAGGAAAACACACCAAGCTGTGCGGT
C5orf56-NSD1	chr5	131785381	chr5	176662822	10	25	THQAVRSEKKRLRKP	CACACCAAGCTGTGCGGTCAGAGAAGAAACGCCTTAGGAAGCCAA
C5orf56-NSD1	chr5	131785381	chr5	176662822	11	26	HQAVRSEKKRLRKPS	ACCAAGCTGTGCGGTCAGAGAAGAAACGCCTTAGGAAGCCAAGCA
C5orf56-NSD1	chr5	131785381	chr5	176662822	9	24	KTHQAVRSEKKRLRK	AAACACACCAAGCTGTGCGGTCAGAGAAGAAACGCCTTAGGAAGC

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Information of the samples that have these potential fusion neoantigens of C5orf56-NSD1

These samples were reported as having these fusion breakpoints. For individual breakpoints, we checked the open reading frames considering multiple gene isoforms and chose the in-frame fusion genes only. Then, we made fusion protein sequences and predicted the fusion neoantigens. These fusion-positive samples may have these potential fusion neoantigens.

Cancer type	Fusion gene	Hchr	Hbp	Henst	Tchr	Tbp	Tenst	Sample
Non-Cancer	C5orf56-NSD1	chr5	131785381	ENST00000337752	chr5	176662822	ENST00000347982	TCGA-BR-6564-11A

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Potential target of CAR-T therapy development for C5orf56-NSD1

Predicted 3D structure. We used RoseTTAFold.

Retention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, to provide the retention of the transmembrane domain, we only show the protein feature retention information of those transmembrane features

* Minus value of BPloci means that the break point is located before the CDS.

- In-frame and retained 'Transmembrane'.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Protein feature

Protein feature note

Subcellular localization prediction of the transmembrane domain retained fusion proteins
* We used DeepLoc 1.0. The order of the X-axis of the barplot is as follows: Entry_ID, Localization, Type, Nucleus, Cytoplasm, Extracellular, Mitochondrion, Cell_membrane, Endoplasmic_reticulum, Plastid, Golgi.apparatus, Lysosome.Vacuole, Peroxisome. Y-axis is the output score of DeepLoc. Clicking the image will open a new tab with a large image.

Hgene

Hchr

Hbp

Henst

Tgene

Tchr

Tbp

Tenst

DeepLoc result

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Related Drugs to C5orf56-NSD1

Drugs used for this fusion-positive patient.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)

Hgene

Tgene

Drug

Source

PMID

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Related Diseases to C5orf56-NSD1

Diseases that have this fusion gene.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)

Hgene

Tgene

Disease

Source

PMID

Diseases associated with fusion partners.
(DisGeNet 4.0)

Partner

Gene

Disease ID

Disease name

# pubmeds

Source

	Fusion Gene and Fusion Protein Summary
	Fusion Amino Acid Sequences (multiple BPs and multiple gene isoforms)
	Fusion Protein Breakpoint Sequences - (for the Screening of the FusionNeoAntigens)
	Potential FusionNeoAntigens in HLA I - (netMHCpan v4.1 + deepHLApan v1.1)
	Potential FusionNeoAntigens in HLA II - (netMHCIIpan v4.1)
	Fusion Breakpoint 14 AA Peptide Structure - (RoseTTAFold)
	Filtering FusionNeoAntigens Through Checking the Interaction with HLAs in 3D - (Glide)
	Vaccine Design for the FusionNeoAntigens (RNA/protein sequences)
	Potential target of CAR-T therapy development
	Information on the samples that have these potential fusion neoantigens
	Fusion Protein Targeting Drugs - (Manual Curation)
	Fusion Protein Related diseases - (Manual Curation)