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Fusion Protein:CAMK2D-CAMK2A |
Fusion Gene and Fusion Protein Summary |
 Fusion gene summary |
| Fusion partner gene information | Fusion gene name: CAMK2D-CAMK2A | FusionPDB ID: 12665 | FusionGDB2.0 ID: 12665 | Hgene | Tgene | Gene symbol | CAMK2D | CAMK2A | Gene ID | 817 | 815 |
| Gene name | calcium/calmodulin dependent protein kinase II delta | calcium/calmodulin dependent protein kinase II alpha | |
| Synonyms | CAMKD | CAMKA|CaMKIINalpha|CaMKIIalpha|MRD53|MRT63 | |
| Cytomap | 4q26 | 5q32 | |
| Type of gene | protein-coding | protein-coding | |
| Description | calcium/calmodulin-dependent protein kinase type II subunit deltaCaM kinase II delta subunitCaM-kinase II delta chainCaMK-II delta subunitcalcium/calmodulin-dependent protein kinase (CaM kinase) II deltacalcium/calmodulin-dependent protein kinase typ | calcium/calmodulin-dependent protein kinase type II subunit alphaCaM kinase II alpha subunitCaM-kinase II alpha chainCaMK-II alpha subunitcaM kinase II subunit alphacaMK-II subunit alphacalcium/calmodulin-dependent protein kinase (CaM kinase) II alp | |
| Modification date | 20200327 | 20200327 | |
| UniProtAcc | Q13557 Main function of 5'-partner protein: FUNCTION: Calcium/calmodulin-dependent protein kinase involved in the regulation of Ca(2+) homeostatis and excitation-contraction coupling (ECC) in heart by targeting ion channels, transporters and accessory proteins involved in Ca(2+) influx into the myocyte, Ca(2+) release from the sarcoplasmic reticulum (SR), SR Ca(2+) uptake and Na(+) and K(+) channel transport. Targets also transcription factors and signaling molecules to regulate heart function. In its activated form, is involved in the pathogenesis of dilated cardiomyopathy and heart failure. Contributes to cardiac decompensation and heart failure by regulating SR Ca(2+) release via direct phosphorylation of RYR2 Ca(2+) channel on 'Ser-2808'. In the nucleus, phosphorylates the MEF2 repressor HDAC4, promoting its nuclear export and binding to 14-3-3 protein, and expression of MEF2 and genes involved in the hypertrophic program. Is essential for left ventricular remodeling responses to myocardial infarction. In pathological myocardial remodeling acts downstream of the beta adrenergic receptor signaling cascade to regulate key proteins involved in ECC. Regulates Ca(2+) influx to myocytes by binding and phosphorylating the L-type Ca(2+) channel subunit beta-2 CACNB2. In addition to Ca(2+) channels, can target and regulate the cardiac sarcolemmal Na(+) channel Nav1.5/SCN5A and the K+ channel Kv4.3/KCND3, which contribute to arrhythmogenesis in heart failure. Phosphorylates phospholamban (PLN/PLB), an endogenous inhibitor of SERCA2A/ATP2A2, contributing to the enhancement of SR Ca(2+) uptake that may be important in frequency-dependent acceleration of relaxation (FDAR) and maintenance of contractile function during acidosis. May participate in the modulation of skeletal muscle function in response to exercise, by regulating SR Ca(2+) transport through phosphorylation of PLN/PLB and triadin, a ryanodine receptor-coupling factor. {ECO:0000269|PubMed:16690701, ECO:0000269|PubMed:17179159}. | . | |
| Ensembl transtripts involved in fusion gene | ENST ids | ENST00000296402, ENST00000342666, ENST00000379773, ENST00000394522, ENST00000394524, ENST00000394526, ENST00000418639, ENST00000429180, ENST00000454265, ENST00000508738, ENST00000514328, ENST00000515496, ENST00000505990, ENST00000509907, ENST00000511664, | ENST00000351010, ENST00000398376, ENST00000348628, |
| Fusion gene scores for assessment (based on all fusion genes of FusionGDB 2.0) | * DoF score | 10 X 12 X 8=960 | 7 X 7 X 6=294 |
| # samples | 13 | 7 | |
| ** MAII score | log2(13/960*10)=-2.88452278258006 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0 | log2(7/294*10)=-2.0703893278914 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0 | |
| Fusion gene context | PubMed: CAMK2D [Title/Abstract] AND CAMK2A [Title/Abstract] AND fusion [Title/Abstract] | ||
| Fusion neoantigen context | PubMed: CAMK2D [Title/Abstract] AND CAMK2A [Title/Abstract] AND neoantigen [Title/Abstract] | ||
| Most frequent breakpoint (based on all fusion genes of FusionGDB 2.0) | CAMK2D(114469812)-CAMK2A(149636208), # samples:1 | ||
| Anticipated loss of major functional domain due to fusion event. | CAMK2D-CAMK2A seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. CAMK2D-CAMK2A seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. CAMK2D-CAMK2A seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF. CAMK2D-CAMK2A seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF. CAMK2D-CAMK2A seems lost the major protein functional domain in Hgene partner, which is a IUPHAR drug target due to the frame-shifted ORF. CAMK2D-CAMK2A seems lost the major protein functional domain in Hgene partner, which is a kinase due to the frame-shifted ORF. CAMK2D-CAMK2A seems lost the major protein functional domain in Tgene partner, which is a essential gene due to the frame-shifted ORF. CAMK2D-CAMK2A seems lost the major protein functional domain in Tgene partner, which is a IUPHAR drug target due to the frame-shifted ORF. CAMK2D-CAMK2A seems lost the major protein functional domain in Tgene partner, which is a kinase due to the frame-shifted ORF. | ||
| * DoF score (Degree of Frequency) = # partners X # break points X # cancer types ** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10) |
| Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez |
| Partner | Gene | GO ID | GO term | PubMed ID |
| Hgene | CAMK2D | GO:0003254 | regulation of membrane depolarization | 22514276 |
| Hgene | CAMK2D | GO:0006468 | protein phosphorylation | 17179159|23283722 |
| Hgene | CAMK2D | GO:0018105 | peptidyl-serine phosphorylation | 22514276|23283722 |
| Hgene | CAMK2D | GO:0018107 | peptidyl-threonine phosphorylation | 22514276|23283722 |
| Hgene | CAMK2D | GO:0046777 | protein autophosphorylation | 22514276 |
| Hgene | CAMK2D | GO:1901897 | regulation of relaxation of cardiac muscle | 23283722 |
| Hgene | CAMK2D | GO:1902306 | negative regulation of sodium ion transmembrane transport | 22514276 |
| Hgene | CAMK2D | GO:2000650 | negative regulation of sodium ion transmembrane transporter activity | 22514276 |
| Tgene | CAMK2A | GO:0006468 | protein phosphorylation | 17052756 |
| Tgene | CAMK2A | GO:0038166 | angiotensin-activated signaling pathway | 20584908 |
| Four levels of functional features of fusion genes Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chr4:114469812/chr5:149636208) - FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels. - How to search 1. Put your fusion gene symbol. 2. Press the tab key until there will be shown the breakpoint information filled. 4. Go down and press 'Search' tab twice. 4. Go down to have the hyperlink of the search result. 5. Click the hyperlink. 6. See the FGviewer result for your fusion gene. |
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| Retention analysis results of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features, are available here. |
| Fusion gene breakpoints across CAMK2D (5'-gene) * Click on the image to open the UCSC genome browser with custom track showing this image in a new window. |
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| Fusion gene breakpoints across CAMK2A (3'-gene) * Click on the image to open the UCSC genome browser with custom track showing this image in a new window. |
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Fusion Amino Acid Sequences |
| Fusion information from ORFfinder translation from full-length transcript sequence from FusionPDB. |
| Henst | Tenst | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand | Seq length (transcript) | BP loci (transcript) | Predicted start (transcript) | Predicted stop (transcript) | Seq length (amino acids) |
| ENST00000454265 | CAMK2D | chr4 | 114469812 | - | ENST00000348628 | CAMK2A | chr5 | 149636208 | - | 5591 | 1273 | 1292 | 2371 | 359 |
| ENST00000394526 | CAMK2D | chr4 | 114469812 | - | ENST00000348628 | CAMK2A | chr5 | 149636208 | - | 5591 | 1273 | 1292 | 2371 | 359 |
| ENST00000429180 | CAMK2D | chr4 | 114469812 | - | ENST00000348628 | CAMK2A | chr5 | 149636208 | - | 5591 | 1273 | 1292 | 2371 | 359 |
| ENST00000296402 | CAMK2D | chr4 | 114469812 | - | ENST00000348628 | CAMK2A | chr5 | 149636208 | - | 5591 | 1273 | 1292 | 2371 | 359 |
| ENST00000418639 | CAMK2D | chr4 | 114469812 | - | ENST00000348628 | CAMK2A | chr5 | 149636208 | - | 5591 | 1273 | 1292 | 2371 | 359 |
| ENST00000342666 | CAMK2D | chr4 | 114469812 | - | ENST00000348628 | CAMK2A | chr5 | 149636208 | - | 4732 | 414 | 433 | 1512 | 359 |
| DeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated. |
| Henst | Tenst | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand | No-coding score | Coding score |
| ENST00000454265 | ENST00000348628 | CAMK2D | chr4 | 114469812 | - | CAMK2A | chr5 | 149636208 | - | 0.001646585 | 0.9983535 |
| ENST00000394526 | ENST00000348628 | CAMK2D | chr4 | 114469812 | - | CAMK2A | chr5 | 149636208 | - | 0.001646585 | 0.9983535 |
| ENST00000429180 | ENST00000348628 | CAMK2D | chr4 | 114469812 | - | CAMK2A | chr5 | 149636208 | - | 0.001646585 | 0.9983535 |
| ENST00000296402 | ENST00000348628 | CAMK2D | chr4 | 114469812 | - | CAMK2A | chr5 | 149636208 | - | 0.001646585 | 0.9983535 |
| ENST00000418639 | ENST00000348628 | CAMK2D | chr4 | 114469812 | - | CAMK2A | chr5 | 149636208 | - | 0.001646585 | 0.9983535 |
| ENST00000342666 | ENST00000348628 | CAMK2D | chr4 | 114469812 | - | CAMK2A | chr5 | 149636208 | - | 0.001280743 | 0.99871933 |
| Predicted full-length fusion amino acid sequences. For individual full-length fusion transcript sequence from FusionPDB, we ran ORFfinder and chose the longest ORF among all the predicted ones. |
Get the fusion protein sequences from here. |
| Fusion protein sequence information is available in the fasta format. >FusionGDB ID_FusionGDB isoform ID_FGname_Hgene_Hchr_Hbp_Henst_Tgene_Tchr_Tbp_Tenst_length(fusion AA) seq_BP |
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Fusion Protein Breakpoint Sequences for CAMK2D-CAMK2A |
| +/-13 AA sequence from the breakpoints of the fusion protein sequences. |
| Hgene | Hchr | Hbp | Tgene | Tchr | Tbp | Length(fusion protein) | BP in fusion protein | Peptide |
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Potential FusionNeoAntigen Information of CAMK2D-CAMK2A in HLA I |
| Multiple sequence alignments of the potential FusionNeoAntigens per fusion breakpoints. If the MSA is empty, then it means that there were predicted fusion neoantigens in this fusion breakpoint, but those predicted fusion neoantigens were not across the breakpoint, which is not fusion-specific. |
| Potential FusionNeoAntigen Information * We used NetMHCpan v4.1 (%rank<0.5) and deepHLApan v1.1 (immunogenic score>0.5) |
| Fusion gene | Hchr | Hbp | Tgene | Tchr | Tbp | HLA I | FusionNeoAntigen peptide | Binding score | Immunogenic score | Neoantigen start (at BP 13) | Neoantigen end (at BP 13) |
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Potential FusionNeoAntigen Information of CAMK2D-CAMK2A in HLA II |
| Multiple sequence alignments of the potential FusionNeoAntigens per fusion breakpoints. If the MSA is empty, then it means that there were predicted fusion neoantigens in this fusion breakpoint, but those predicted fusion neoantigens were not across the breakpoint, which is not fusion-specific. |
| Potential FusionNeoAntigen Information * We used NetMHCIIpan v4.1 (%rank<0.5). |
| Fusion gene | Hchr | Hbp | Tgene | Tchr | Tbp | HLA II | FusionNeoAntigen peptide | Neoantigen start (at BP 13) | Neoantigen end (at BP 13) |
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Fusion breakpoint peptide structures of CAMK2D-CAMK2A |
| 3D structures of the fusion breakpoint peptide of 14AA sequence that have potential fusion neoantigens * The minimum length of the amino acid sequence in RoseTTAFold is 14AA. Here, we predicted the 14AA fusion protein breakpoint sequence not the fusion neoantigen peptide, which is shorter than 14 AA. |
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Filtering FusionNeoAntigens Through Checking the Interaction with HLAs in 3D of CAMK2D-CAMK2A |
| Virtual screening between 25 HLAs (from PDB) and FusionNeoAntigens * We used Glide to predict the interaction between HLAs and neoantigens. |
| HLA allele | PDB ID | File name | BPseq | Docking score | Glide score |
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Vaccine Design for the FusionNeoAntigens of CAMK2D-CAMK2A |
| mRNA and peptide sequences of FusionNeoAntigens that have potential interaction with HLA-Is. |
| Fusion gene | Hchr | Hbp | Tchr | Tbp | Start in +/-13AA | End in +/-13AA | FusionNeoAntigen peptide sequence | FusionNeoAntigen RNA sequence |
| mRNA and peptide sequences of FusionNeoAntigens that have potential interaction with HLA-IIs. |
| Fusion gene | Hchr | Hbp | Tchr | Tbp | Start in +/-13AA | End in +/-13AA | FusionNeoAntigen peptide | FusionNEoAntigen RNA sequence |
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Information of the samples that have these potential fusion neoantigens of CAMK2D-CAMK2A |
| These samples were reported as having these fusion breakpoints. For individual breakpoints, we checked the open reading frames considering multiple gene isoforms and chose the in-frame fusion genes only. Then, we made fusion protein sequences and predicted the fusion neoantigens. These fusion-positive samples may have these potential fusion neoantigens. |
| Cancer type | Fusion gene | Hchr | Hbp | Henst | Tchr | Tbp | Tenst | Sample |
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Potential target of CAR-T therapy development for CAMK2D-CAMK2A |
| Predicted 3D structure. We used RoseTTAFold. |
| Retention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, to provide the retention of the transmembrane domain, we only show the protein feature retention information of those transmembrane features * Minus value of BPloci means that the break point is located before the CDS. |
| - In-frame and retained 'Transmembrane'. |
| Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Protein feature | Protein feature note |
| Subcellular localization prediction of the transmembrane domain retained fusion proteins * We used DeepLoc 1.0. The order of the X-axis of the barplot is as follows: Entry_ID, Localization, Type, Nucleus, Cytoplasm, Extracellular, Mitochondrion, Cell_membrane, Endoplasmic_reticulum, Plastid, Golgi.apparatus, Lysosome.Vacuole, Peroxisome. Y-axis is the output score of DeepLoc. Clicking the image will open a new tab with a large image. |
| Hgene | Hchr | Hbp | Henst | Tgene | Tchr | Tbp | Tenst | DeepLoc result |
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Related Drugs to CAMK2D-CAMK2A |
| Drugs used for this fusion-positive patient. (Manual curation of PubMed, 04-30-2022 + MyCancerGenome) |
| Hgene | Tgene | Drug | Source | PMID |
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Related Diseases to CAMK2D-CAMK2A |
| Diseases that have this fusion gene. (Manual curation of PubMed, 04-30-2022 + MyCancerGenome) |
| Hgene | Tgene | Disease | Source | PMID |
| Diseases associated with fusion partners. (DisGeNet 4.0) |
| Partner | Gene | Disease ID | Disease name | # pubmeds | Source |
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