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Fusion Protein:CDC42BPA-EPRS |
Fusion Gene and Fusion Protein Summary |
 Fusion gene summary |
| Fusion partner gene information | Fusion gene name: CDC42BPA-EPRS | FusionPDB ID: 14836 | FusionGDB2.0 ID: 14836 | Hgene | Tgene | Gene symbol | CDC42BPA | EPRS | Gene ID | 8476 | 2058 |
| Gene name | CDC42 binding protein kinase alpha | glutamyl-prolyl-tRNA synthetase 1 | |
| Synonyms | MRCK|MRCKA|PK428 | EARS|EPRS|GLUPRORS|HLD15|PARS|PIG32|QARS|QPRS | |
| Cytomap | 1q42.13 | 1q41 | |
| Type of gene | protein-coding | protein-coding | |
| Description | serine/threonine-protein kinase MRCK alphaCDC42 binding protein kinase alpha (DMPK-like)CDC42 binding protein kinase betamyotonic dystrophy kinase-related CDC42-binding protein kinase alphamyotonic dystrophy protein kinase-like alphaser-thr protein k | bifunctional glutamate/proline--tRNA ligasebifunctional aminoacyl-tRNA synthetasecell proliferation-inducing gene 32 proteinglutamate tRNA ligaseglutamatyl-prolyl-tRNA synthetaseglutaminyl-tRNA synthetaseproliferation-inducing gene 32 proteinprolif | |
| Modification date | 20200313 | 20200313 | |
| UniProtAcc | Q5VT25 Main function of 5'-partner protein: FUNCTION: Serine/threonine-protein kinase which is an important downstream effector of CDC42 and plays a role in the regulation of cytoskeleton reorganization and cell migration (PubMed:15723050, PubMed:9418861, PubMed:9092543). Regulates actin cytoskeletal reorganization via phosphorylation of PPP1R12C and MYL9/MLC2 (PubMed:21457715). In concert with MYO18A and LURAP1, is involved in modulating lamellar actomyosin retrograde flow that is crucial to cell protrusion and migration (PubMed:18854160). Phosphorylates: PPP1R12A, LIMK1 and LIMK2 (PubMed:11340065, PubMed:11399775). May play a role in TFRC-mediated iron uptake (PubMed:20188707). In concert with FAM89B/LRAP25 mediates the targeting of LIMK1 to the lamellipodium resulting in its activation and subsequent phosphorylation of CFL1 which is important for lamellipodial F-actin regulation (By similarity). Triggers the formation of an extrusion apical actin ring required for epithelial extrusion of apoptotic cells (PubMed:29162624). {ECO:0000250|UniProtKB:Q3UU96, ECO:0000269|PubMed:11340065, ECO:0000269|PubMed:11399775, ECO:0000269|PubMed:15723050, ECO:0000269|PubMed:18854160, ECO:0000269|PubMed:20188707, ECO:0000269|PubMed:21457715, ECO:0000269|PubMed:29162624, ECO:0000269|PubMed:9092543, ECO:0000269|PubMed:9418861}. | P07814 Main function of 5'-partner protein: FUNCTION: Multifunctional protein which is primarily part of the aminoacyl-tRNA synthetase multienzyme complex, also know as multisynthetase complex, that catalyzes the attachment of the cognate amino acid to the corresponding tRNA in a two-step reaction: the amino acid is first activated by ATP to form a covalent intermediate with AMP and is then transferred to the acceptor end of the cognate tRNA (PubMed:1756734, PubMed:24100331, PubMed:23263184). The phosphorylation of EPRS1, induced by interferon-gamma, dissociates the protein from the aminoacyl-tRNA synthetase multienzyme complex and recruits it to the GAIT complex that binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin), suppressing their translation. Interferon-gamma can therefore redirect, in specific cells, the EPRS1 function from protein synthesis to translation inhibition (PubMed:15479637, PubMed:23071094). Also functions as an effector of the mTORC1 signaling pathway by promoting, through SLC27A1, the uptake of long-chain fatty acid by adipocytes. Thereby, it also plays a role in fat metabolism and more indirectly influences lifespan (PubMed:28178239). {ECO:0000269|PubMed:15479637, ECO:0000269|PubMed:1756734, ECO:0000269|PubMed:23071094, ECO:0000269|PubMed:23263184, ECO:0000269|PubMed:24100331, ECO:0000269|PubMed:28178239}. | |
| Ensembl transtripts involved in fusion gene | ENST ids | ENST00000334218, ENST00000366764, ENST00000366765, ENST00000366766, ENST00000366767, ENST00000366769, ENST00000535525, ENST00000488131, | ENST00000468487, ENST00000366923, |
| Fusion gene scores for assessment (based on all fusion genes of FusionGDB 2.0) | * DoF score | 14 X 12 X 9=1512 | 9 X 9 X 4=324 |
| # samples | 15 | 9 | |
| ** MAII score | log2(15/1512*10)=-3.33342373372519 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0 | log2(9/324*10)=-1.84799690655495 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0 | |
| Fusion gene context | PubMed: CDC42BPA [Title/Abstract] AND EPRS [Title/Abstract] AND fusion [Title/Abstract] | ||
| Fusion neoantigen context | PubMed: CDC42BPA [Title/Abstract] AND EPRS [Title/Abstract] AND neoantigen [Title/Abstract] | ||
| Most frequent breakpoint (based on all fusion genes of FusionGDB 2.0) | CDC42BPA(227203781)-EPRS(220180680), # samples:3 | ||
| Anticipated loss of major functional domain due to fusion event. | CDC42BPA-EPRS seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. CDC42BPA-EPRS seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. CDC42BPA-EPRS seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF. CDC42BPA-EPRS seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF. | ||
| * DoF score (Degree of Frequency) = # partners X # break points X # cancer types ** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10) |
| Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez |
| Partner | Gene | GO ID | GO term | PubMed ID |
| Hgene | CDC42BPA | GO:0006468 | protein phosphorylation | 9092543 |
| Hgene | CDC42BPA | GO:0031532 | actin cytoskeleton reorganization | 9418861 |
| Tgene | EPRS | GO:0006433 | prolyl-tRNA aminoacylation | 24100331 |
| Tgene | EPRS | GO:0017148 | negative regulation of translation | 23071094 |
| Tgene | EPRS | GO:0071346 | cellular response to interferon-gamma | 15479637 |
| Four levels of functional features of fusion genes Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chr1:227203781/chr1:220180680) - FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels. - How to search 1. Put your fusion gene symbol. 2. Press the tab key until there will be shown the breakpoint information filled. 4. Go down and press 'Search' tab twice. 4. Go down to have the hyperlink of the search result. 5. Click the hyperlink. 6. See the FGviewer result for your fusion gene. |
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| Retention analysis results of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features, are available here. |
| Fusion gene breakpoints across CDC42BPA (5'-gene) * Click on the image to open the UCSC genome browser with custom track showing this image in a new window. |
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| Fusion gene breakpoints across EPRS (3'-gene) * Click on the image to open the UCSC genome browser with custom track showing this image in a new window. |
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Fusion Amino Acid Sequences |
| Fusion information from ORFfinder translation from full-length transcript sequence from FusionPDB. |
| Henst | Tenst | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand | Seq length (transcript) | BP loci (transcript) | Predicted start (transcript) | Predicted stop (transcript) | Seq length (amino acids) |
| ENST00000366767 | CDC42BPA | chr1 | 227203781 | - | ENST00000366923 | EPRS | chr1 | 220180680 | - | 7648 | 4509 | 0 | 7442 | 2480 |
| ENST00000366769 | CDC42BPA | chr1 | 227203781 | - | ENST00000366923 | EPRS | chr1 | 220180680 | - | 9183 | 6044 | 1292 | 8977 | 2561 |
| ENST00000366764 | CDC42BPA | chr1 | 227203781 | - | ENST00000366923 | EPRS | chr1 | 220180680 | - | 8750 | 5611 | 943 | 8544 | 2533 |
| ENST00000334218 | CDC42BPA | chr1 | 227203781 | - | ENST00000366923 | EPRS | chr1 | 220180680 | - | 8834 | 5695 | 943 | 8628 | 2561 |
| ENST00000366766 | CDC42BPA | chr1 | 227203781 | - | ENST00000366923 | EPRS | chr1 | 220180680 | - | 8939 | 5800 | 943 | 8733 | 2596 |
| ENST00000535525 | CDC42BPA | chr1 | 227203781 | - | ENST00000366923 | EPRS | chr1 | 220180680 | - | 7858 | 4719 | 27 | 7652 | 2541 |
| ENST00000366765 | CDC42BPA | chr1 | 227203781 | - | ENST00000366923 | EPRS | chr1 | 220180680 | - | 8462 | 5323 | 532 | 8256 | 2574 |
| DeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated. |
| Henst | Tenst | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand | No-coding score | Coding score |
| ENST00000366767 | ENST00000366923 | CDC42BPA | chr1 | 227203781 | - | EPRS | chr1 | 220180680 | - | 0.000452524 | 0.9995474 |
| ENST00000366769 | ENST00000366923 | CDC42BPA | chr1 | 227203781 | - | EPRS | chr1 | 220180680 | - | 0.001576272 | 0.9984237 |
| ENST00000366764 | ENST00000366923 | CDC42BPA | chr1 | 227203781 | - | EPRS | chr1 | 220180680 | - | 0.001100391 | 0.9988996 |
| ENST00000334218 | ENST00000366923 | CDC42BPA | chr1 | 227203781 | - | EPRS | chr1 | 220180680 | - | 0.001078157 | 0.9989219 |
| ENST00000366766 | ENST00000366923 | CDC42BPA | chr1 | 227203781 | - | EPRS | chr1 | 220180680 | - | 0.001101106 | 0.99889886 |
| ENST00000535525 | ENST00000366923 | CDC42BPA | chr1 | 227203781 | - | EPRS | chr1 | 220180680 | - | 0.000555753 | 0.9994442 |
| ENST00000366765 | ENST00000366923 | CDC42BPA | chr1 | 227203781 | - | EPRS | chr1 | 220180680 | - | 0.000712139 | 0.9992879 |
| Predicted full-length fusion amino acid sequences. For individual full-length fusion transcript sequence from FusionPDB, we ran ORFfinder and chose the longest ORF among all the predicted ones. |
Get the fusion protein sequences from here. |
| Fusion protein sequence information is available in the fasta format. >FusionGDB ID_FusionGDB isoform ID_FGname_Hgene_Hchr_Hbp_Henst_Tgene_Tchr_Tbp_Tenst_length(fusion AA) seq_BP |
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Fusion Protein Breakpoint Sequences for CDC42BPA-EPRS |
| +/-13 AA sequence from the breakpoints of the fusion protein sequences. |
| Hgene | Hchr | Hbp | Tgene | Tchr | Tbp | Length(fusion protein) | BP in fusion protein | Peptide |
| CDC42BPA | chr1 | 227203781 | EPRS | chr1 | 220180680 | 4509 | 1503 | PGDGIQILKDLPMNPEVGLKPVWYSP |
| CDC42BPA | chr1 | 227203781 | EPRS | chr1 | 220180680 | 4509 | 1780 | KPGNPPAEIGQNISSNSSASILESKS |
| CDC42BPA | chr1 | 227203781 | EPRS | chr1 | 220180680 | 4719 | 1564 | PGDGIQILKDLPMNPEVGLKPVWYSP |
| CDC42BPA | chr1 | 227203781 | EPRS | chr1 | 220180680 | 4719 | 1841 | KPGNPPAEIGQNISSNSSASILESKS |
| CDC42BPA | chr1 | 227203781 | EPRS | chr1 | 220180680 | 5323 | 1597 | PGDGIQILKDLPMNPEVGLKPVWYSP |
| CDC42BPA | chr1 | 227203781 | EPRS | chr1 | 220180680 | 5323 | 1874 | KPGNPPAEIGQNISSNSSASILESKS |
| CDC42BPA | chr1 | 227203781 | EPRS | chr1 | 220180680 | 5611 | 1556 | PGDGIQILKDLPMNPEVGLKPVWYSP |
| CDC42BPA | chr1 | 227203781 | EPRS | chr1 | 220180680 | 5611 | 1833 | KPGNPPAEIGQNISSNSSASILESKS |
| CDC42BPA | chr1 | 227203781 | EPRS | chr1 | 220180680 | 5695 | 1584 | PGDGIQILKDLPMNPEVGLKPVWYSP |
| CDC42BPA | chr1 | 227203781 | EPRS | chr1 | 220180680 | 5695 | 1861 | KPGNPPAEIGQNISSNSSASILESKS |
| CDC42BPA | chr1 | 227203781 | EPRS | chr1 | 220180680 | 5800 | 1619 | PGDGIQILKDLPMNPEVGLKPVWYSP |
| CDC42BPA | chr1 | 227203781 | EPRS | chr1 | 220180680 | 5800 | 1896 | KPGNPPAEIGQNISSNSSASILESKS |
| CDC42BPA | chr1 | 227203781 | EPRS | chr1 | 220180680 | 6044 | 1584 | PGDGIQILKDLPMNPEVGLKPVWYSP |
| CDC42BPA | chr1 | 227203781 | EPRS | chr1 | 220180680 | 6044 | 1861 | KPGNPPAEIGQNISSNSSASILESKS |
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Potential FusionNeoAntigen Information of CDC42BPA-EPRS in HLA I |
| Multiple sequence alignments of the potential FusionNeoAntigens per fusion breakpoints. If the MSA is empty, then it means that there were predicted fusion neoantigens in this fusion breakpoint, but those predicted fusion neoantigens were not across the breakpoint, which is not fusion-specific. |
| Potential FusionNeoAntigen Information * We used NetMHCpan v4.1 (%rank<0.5) and deepHLApan v1.1 (immunogenic score>0.5) |
| Fusion gene | Hchr | Hbp | Tgene | Tchr | Tbp | HLA I | FusionNeoAntigen peptide | Binding score | Immunogenic score | Neoantigen start (at BP 13) | Neoantigen end (at BP 13) |
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Potential FusionNeoAntigen Information of CDC42BPA-EPRS in HLA II |
| Multiple sequence alignments of the potential FusionNeoAntigens per fusion breakpoints. If the MSA is empty, then it means that there were predicted fusion neoantigens in this fusion breakpoint, but those predicted fusion neoantigens were not across the breakpoint, which is not fusion-specific. |
| Potential FusionNeoAntigen Information * We used NetMHCIIpan v4.1 (%rank<0.5). |
| Fusion gene | Hchr | Hbp | Tgene | Tchr | Tbp | HLA II | FusionNeoAntigen peptide | Neoantigen start (at BP 13) | Neoantigen end (at BP 13) |
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Fusion breakpoint peptide structures of CDC42BPA-EPRS |
| 3D structures of the fusion breakpoint peptide of 14AA sequence that have potential fusion neoantigens * The minimum length of the amino acid sequence in RoseTTAFold is 14AA. Here, we predicted the 14AA fusion protein breakpoint sequence not the fusion neoantigen peptide, which is shorter than 14 AA. |
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Filtering FusionNeoAntigens Through Checking the Interaction with HLAs in 3D of CDC42BPA-EPRS |
| Virtual screening between 25 HLAs (from PDB) and FusionNeoAntigens * We used Glide to predict the interaction between HLAs and neoantigens. |
| HLA allele | PDB ID | File name | BPseq | Docking score | Glide score |
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Vaccine Design for the FusionNeoAntigens of CDC42BPA-EPRS |
| mRNA and peptide sequences of FusionNeoAntigens that have potential interaction with HLA-Is. |
| Fusion gene | Hchr | Hbp | Tchr | Tbp | Start in +/-13AA | End in +/-13AA | FusionNeoAntigen peptide sequence | FusionNeoAntigen RNA sequence |
| mRNA and peptide sequences of FusionNeoAntigens that have potential interaction with HLA-IIs. |
| Fusion gene | Hchr | Hbp | Tchr | Tbp | Start in +/-13AA | End in +/-13AA | FusionNeoAntigen peptide | FusionNEoAntigen RNA sequence |
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Information of the samples that have these potential fusion neoantigens of CDC42BPA-EPRS |
| These samples were reported as having these fusion breakpoints. For individual breakpoints, we checked the open reading frames considering multiple gene isoforms and chose the in-frame fusion genes only. Then, we made fusion protein sequences and predicted the fusion neoantigens. These fusion-positive samples may have these potential fusion neoantigens. |
| Cancer type | Fusion gene | Hchr | Hbp | Henst | Tchr | Tbp | Tenst | Sample |
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Potential target of CAR-T therapy development for CDC42BPA-EPRS |
| Predicted 3D structure. We used RoseTTAFold. |
| Retention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, to provide the retention of the transmembrane domain, we only show the protein feature retention information of those transmembrane features * Minus value of BPloci means that the break point is located before the CDS. |
| - In-frame and retained 'Transmembrane'. |
| Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Protein feature | Protein feature note |
| Subcellular localization prediction of the transmembrane domain retained fusion proteins * We used DeepLoc 1.0. The order of the X-axis of the barplot is as follows: Entry_ID, Localization, Type, Nucleus, Cytoplasm, Extracellular, Mitochondrion, Cell_membrane, Endoplasmic_reticulum, Plastid, Golgi.apparatus, Lysosome.Vacuole, Peroxisome. Y-axis is the output score of DeepLoc. Clicking the image will open a new tab with a large image. |
| Hgene | Hchr | Hbp | Henst | Tgene | Tchr | Tbp | Tenst | DeepLoc result |
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Related Drugs to CDC42BPA-EPRS |
| Drugs used for this fusion-positive patient. (Manual curation of PubMed, 04-30-2022 + MyCancerGenome) |
| Hgene | Tgene | Drug | Source | PMID |
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Related Diseases to CDC42BPA-EPRS |
| Diseases that have this fusion gene. (Manual curation of PubMed, 04-30-2022 + MyCancerGenome) |
| Hgene | Tgene | Disease | Source | PMID |
| Diseases associated with fusion partners. (DisGeNet 4.0) |
| Partner | Gene | Disease ID | Disease name | # pubmeds | Source |
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