Home

Download

Statistics

Examples

Help

Contact

Fusion Protein:CHKA-SLC5A12

Fusion Gene and Fusion Protein Summary

Fusion gene summary

Fusion partner gene information	Fusion gene name: CHKA-SLC5A12
	FusionPDB ID: 16504
	FusionGDB2.0 ID: 16504
		Hgene	Tgene
	Gene symbol	CHKA	SLC5A12
	Gene ID	1119	159963
	Gene name	choline kinase alpha	solute carrier family 5 member 12
	Synonyms	CHK\|CK\|CKI\|EK	SMCT2
	Cytomap	11q13.2	11p14.2
	Type of gene	protein-coding	protein-coding
	Description	choline kinase alphaCHETK-alphaethanolamine kinase	sodium-coupled monocarboxylate transporter 2electroneutral sodium monocarboxylate cotransporterlow-affinity sodium-lactate cotransportersodium-iodide related cotransportersolute carrier family 5 (sodium/glucose cotransporter), member 12solute carrier
	Modification date	20200313	20200313
	UniProtAcc	P35790 Main function of 5'-partner protein: FUNCTION: Has a key role in phospholipid biosynthesis and may contribute to tumor cell growth. Catalyzes the first step in phosphatidylcholine biosynthesis. Contributes to phosphatidylethanolamine biosynthesis. Phosphorylates choline and ethanolamine. Has higher activity with choline. {ECO:0000269\|PubMed:19915674}.	.
Ensembl transtripts involved in fusion gene	ENST ids	ENST00000265689, ENST00000356135, ENST00000533728,	ENST00000280467, ENST00000396005,
Fusion gene scores for assessment (based on all fusion genes of FusionGDB 2.0)	* DoF score	12 X 7 X 5=420	2 X 1 X 1=2
	# samples	13	2
	** MAII score	log2(13/420*10)=-1.69187770463767 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0	log2(2/2*10)=3.32192809488736
Fusion gene context	PubMed: CHKA [Title/Abstract] AND SLC5A12 [Title/Abstract] AND fusion [Title/Abstract]
Fusion neoantigen context	PubMed: CHKA [Title/Abstract] AND SLC5A12 [Title/Abstract] AND neoantigen [Title/Abstract]
Most frequent breakpoint (based on all fusion genes of FusionGDB 2.0)	CHKA(67864486)-SLC5A12(26734253), # samples:1
Anticipated loss of major functional domain due to fusion event.	CHKA-SLC5A12 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. CHKA-SLC5A12 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF.

* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez

Partner	Gene	GO ID	GO term	PubMed ID
Hgene	CHKA	GO:0006646	phosphatidylethanolamine biosynthetic process	19915674
Hgene	CHKA	GO:0006656	phosphatidylcholine biosynthetic process	19915674

Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chr11:67864486/chr11:26734253)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
4. Go down and press 'Search' tab twice.
4. Go down to have the hyperlink of the search result.
5. Click the hyperlink.
6. See the FGviewer result for your fusion gene.

Retention analysis results of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features, are available here.

Fusion gene breakpoints across CHKA (5'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

Fusion gene breakpoints across SLC5A12 (3'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

Top

Fusion Amino Acid Sequences

Fusion information from ORFfinder translation from full-length transcript sequence from FusionPDB.

Henst	Tenst	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	Seq length (transcript)	BP loci (transcript)	Predicted start (transcript)	Predicted stop (transcript)	Seq length (amino acids)
ENST00000265689	CHKA	chr11	67864486	-	ENST00000396005	SLC5A12	chr11	26734253	-	6090	489	18	2006	662
ENST00000265689	CHKA	chr11	67864486	-	ENST00000280467	SLC5A12	chr11	26734253	-	2134	489	18	1340	440
ENST00000356135	CHKA	chr11	67864486	-	ENST00000396005	SLC5A12	chr11	26734253	-	6330	729	210	2246	678
ENST00000356135	CHKA	chr11	67864486	-	ENST00000280467	SLC5A12	chr11	26734253	-	2374	729	210	1580	456

DeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.

Henst	Tenst	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	No-coding score	Coding score
ENST00000265689	ENST00000396005	CHKA	chr11	67864486	-	SLC5A12	chr11	26734253	-	0.003744446	0.9962555
ENST00000265689	ENST00000280467	CHKA	chr11	67864486	-	SLC5A12	chr11	26734253	-	0.024629189	0.97537076
ENST00000356135	ENST00000396005	CHKA	chr11	67864486	-	SLC5A12	chr11	26734253	-	0.003994452	0.9960056
ENST00000356135	ENST00000280467	CHKA	chr11	67864486	-	SLC5A12	chr11	26734253	-	0.037096344	0.9629037

Predicted full-length fusion amino acid sequences. For individual full-length fusion transcript sequence from FusionPDB, we ran ORFfinder and chose the longest ORF among all the predicted ones.

Get the fusion protein sequences from here.

Fusion protein sequence information is available in the fasta format.
>FusionGDB ID_FusionGDB isoform ID_FGname_Hgene_Hchr_Hbp_Henst_Tgene_Tchr_Tbp_Tenst_length(fusion AA) seq_BP

Top

Fusion Protein Breakpoint Sequences for CHKA-SLC5A12

+/-13 AA sequence from the breakpoints of the fusion protein sequences.

Hgene	Hchr	Hbp	Tgene	Tchr	Tbp	Length(fusion protein)	BP in fusion protein	Peptide
CHKA	chr11	67864486	SLC5A12	chr11	26734253	489	157	KVLLRLYGAILQMYLQLRFNKPVRYA
CHKA	chr11	67864486	SLC5A12	chr11	26734253	489	60	SKQLGGQQPPLALPPPPPLPLPLPLP
CHKA	chr11	67864486	SLC5A12	chr11	26734253	729	173	KVLLRLYGAILQMYLQLRFNKPVRYA
CHKA	chr11	67864486	SLC5A12	chr11	26734253	729	76	SKQLGGQQPPLALPPPPPLPLPLPLP

Top

Potential FusionNeoAntigen Information of CHKA-SLC5A12 in HLA I

Multiple sequence alignments of the potential FusionNeoAntigens per fusion breakpoints. If the MSA is empty, then it means that there were predicted fusion neoantigens in this fusion breakpoint, but those predicted fusion neoantigens were not across the breakpoint, which is not fusion-specific.

CHKA-SLC5A12_67864486_26734253.msa

Potential FusionNeoAntigen Information
* We used NetMHCpan v4.1 (%rank<0.5) and deepHLApan v1.1 (immunogenic score>0.5)

Fusion gene	Hchr	Hbp	Tgene	Tchr	Tbp	HLA I	FusionNeoAntigen peptide	Binding score	Immunogenic score	Neoantigen start (at BP 13)	Neoantigen end (at BP 13)
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-B51:02	LALPPPPPL	0.9955	0.933	10	19
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-B51:01	LALPPPPPL	0.9954	0.9593	10	19
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-B15:01	LQMYLQLRF	0.995	0.904	10	19
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-B52:01	LALPPPPPL	0.9945	0.9982	10	19
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-B35:03	LALPPPPPL	0.9939	0.967	10	19
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-B35:05	LALPPPPPL	0.9868	0.8905	10	19
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-B35:04	LALPPPPPL	0.9848	0.9907	10	19
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-B35:02	LALPPPPPL	0.9848	0.9907	10	19
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-B35:01	LALPPPPPL	0.9691	0.9633	10	19
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-B81:01	LALPPPPPL	0.9667	0.7236	10	19
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-B48:01	LALPPPPPL	0.9587	0.7758	10	19
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-B15:17	LALPPPPPL	0.9307	0.9847	10	19
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-B15:25	LQMYLQLRF	0.9269	0.9448	10	19
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-B15:16	LALPPPPPL	0.9251	0.96	10	19
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-A74:03	ILQMYLQLR	0.9121	0.6424	9	18
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-A74:09	ILQMYLQLR	0.9121	0.6424	9	18
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-A74:11	ILQMYLQLR	0.9121	0.6424	9	18
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-A02:04	AILQMYLQL	0.8932	0.8861	8	17
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-A02:17	AILQMYLQL	0.8811	0.8087	8	17
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-A02:21	LALPPPPPL	0.871	0.8325	10	19
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-A32:13	LQMYLQLRF	0.8602	0.9693	10	19
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-A31:02	ILQMYLQLR	0.8454	0.6618	9	18
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-B46:01	LALPPPPPL	0.7974	0.8214	10	19
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-B47:01	LQMYLQLRF	0.7865	0.7238	10	19
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-B15:03	LQMYLQLRF	0.7632	0.8353	10	19
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-B07:10	LALPPPPPL	0.7085	0.7466	10	19
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-A24:14	LQMYLQLRF	0.6712	0.542	10	19
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-B13:01	LQMYLQLRF	0.5854	0.9831	10	19
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-B82:01	LALPPPPPL	0.5104	0.8137	10	19
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-B52:01	LQMYLQLRF	0.159	0.9586	10	19
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-B15:01	RLYGAILQMY	0.9999	0.958	4	14
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-B15:25	RLYGAILQMY	0.9977	0.973	4	14
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-A80:01	RLYGAILQMY	0.9964	0.6101	4	14
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-A02:22	ALPPPPPLPL	0.9916	0.8951	11	21
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-B15:02	RLYGAILQMY	0.991	0.9746	4	14
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-A02:13	ALPPPPPLPL	0.9899	0.9199	11	21
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-A02:27	ALPPPPPLPL	0.9858	0.8803	11	21
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-A02:38	ALPPPPPLPL	0.9797	0.8664	11	21
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-A02:17	ALPPPPPLPL	0.9766	0.8577	11	21
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-A02:16	ALPPPPPLPL	0.9762	0.8656	11	21
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-A02:04	ALPPPPPLPL	0.9759	0.9593	11	21
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-A02:21	ALPPPPPLPL	0.9752	0.9032	11	21
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-A02:11	ALPPPPPLPL	0.9748	0.8682	11	21
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-A02:30	ALPPPPPLPL	0.973	0.8663	11	21
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-A02:24	ALPPPPPLPL	0.973	0.8663	11	21
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-A02:67	ALPPPPPLPL	0.973	0.8663	11	21
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-A02:60	ALPPPPPLPL	0.9723	0.8724	11	21
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-A24:17	RLYGAILQMY	0.9688	0.5314	4	14
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-A32:13	RLYGAILQMY	0.9457	0.9731	4	14
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-A02:19	ALPPPPPLPL	0.933	0.8545	11	21
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-A02:35	ALPPPPPLPL	0.9282	0.8631	11	21
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-A74:09	RLYGAILQMY	0.9263	0.6368	4	14
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-A74:11	RLYGAILQMY	0.9263	0.6368	4	14
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-A74:03	RLYGAILQMY	0.9263	0.6368	4	14
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-A02:29	ALPPPPPLPL	0.8667	0.8666	11	21
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-A02:20	ALPPPPPLPL	0.847	0.8694	11	21
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-B81:01	ALPPPPPLPL	0.8284	0.726	11	21
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-B07:02	ALPPPPPLPL	0.8132	0.7626	11	21
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-B07:05	ALPPPPPLPL	0.7999	0.6815	11	21
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-B35:03	ALPPPPPLPL	0.7396	0.9755	11	21
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-B82:01	ALPPPPPLPL	0.6723	0.844	11	21
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-B27:02	LRLYGAILQMY	0.9999	0.7538	3	14
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-B07:02	LPPPPPLPLPL	0.9994	0.6764	12	23
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-B07:05	LPPPPPLPLPL	0.9993	0.6333	12	23
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-B51:02	LALPPPPPLPL	0.9991	0.9562	10	21
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-B35:03	LALPPPPPLPL	0.9989	0.9539	10	21
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-B35:02	LALPPPPPLPL	0.9972	0.9873	10	21
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-B35:04	LALPPPPPLPL	0.9972	0.9873	10	21
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-A02:13	RLYGAILQMYL	0.9968	0.6308	4	15
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-A02:27	RLYGAILQMYL	0.9965	0.5071	4	15
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-A02:17	RLYGAILQMYL	0.9964	0.5576	4	15
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-A02:04	RLYGAILQMYL	0.9964	0.6539	4	15
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-B35:03	LPPPPPLPLPL	0.9959	0.9041	12	23
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-B51:02	LPPPPPLPLPL	0.9922	0.8205	12	23
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-B81:01	LALPPPPPLPL	0.9897	0.8002	10	21
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-B35:04	LPPPPPLPLPL	0.9778	0.9893	12	23
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-B35:02	LPPPPPLPLPL	0.9778	0.9893	12	23
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-A02:35	RLYGAILQMYL	0.9651	0.5075	4	15
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-B81:01	LPPPPPLPLPL	0.9608	0.7772	12	23
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-A80:01	LRLYGAILQMY	0.9586	0.6474	3	14
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-B07:05	LALPPPPPLPL	0.9361	0.7208	10	21
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-B07:02	LALPPPPPLPL	0.9259	0.7742	10	21
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-B82:01	LPPPPPLPLPL	0.8806	0.7139	12	23
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-B82:01	LALPPPPPLPL	0.814	0.9349	10	21
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-A02:07	ALPPPPPL	0.9856	0.8547	11	19
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-C01:17	ALPPPPPL	0.9792	0.9876	11	19
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-C01:30	ALPPPPPL	0.9702	0.9817	11	19
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-C03:08	LALPPPPPL	0.9996	0.9685	10	19
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-C03:19	LALPPPPPL	0.9995	0.9968	10	19
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-C03:07	LALPPPPPL	0.9995	0.9947	10	19
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-C15:04	LALPPPPPL	0.9993	0.9832	10	19
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-C15:06	LALPPPPPL	0.9988	0.984	10	19
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-C04:06	LALPPPPPL	0.9981	0.9843	10	19
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-B51:07	LALPPPPPL	0.9955	0.9979	10	19
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-B78:01	LALPPPPPL	0.9949	0.9854	10	19
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-C08:04	LALPPPPPL	0.9943	0.9978	10	19
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-C08:13	LALPPPPPL	0.9943	0.9978	10	19
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-B56:04	LALPPPPPL	0.9905	0.7732	10	19
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-C03:14	LALPPPPPL	0.9864	0.9952	10	19
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-B35:12	LALPPPPPL	0.9848	0.9907	10	19
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-C12:12	LALPPPPPL	0.9804	0.9874	10	19
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-B51:08	LALPPPPPL	0.9773	0.9265	10	19
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-C08:03	LALPPPPPL	0.9755	0.999	10	19
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-C06:03	LALPPPPPL	0.9732	0.9987	10	19
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-C02:06	LALPPPPPL	0.971	0.9959	10	19
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-C12:04	LALPPPPPL	0.9697	0.9987	10	19
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-B15:07	LQMYLQLRF	0.9654	0.683	10	19
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-C04:14	LALPPPPPL	0.9597	0.9888	10	19
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-C07:13	LALPPPPPL	0.9596	0.968	10	19
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-C07:29	LALPPPPPL	0.9395	0.9742	10	19
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-B15:04	LQMYLQLRF	0.9358	0.9143	10	19
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-B39:10	LALPPPPPL	0.9187	0.9925	10	19
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-B07:04	LALPPPPPL	0.8904	0.7371	10	19
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-B42:02	LALPPPPPL	0.8713	0.8746	10	19
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-B15:05	LQMYLQLRF	0.8255	0.9438	10	19
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-A02:07	ALPPPPPLP	0.8025	0.8537	11	20
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-C01:30	LALPPPPPL	0.7474	0.9826	10	19
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-B07:12	LALPPPPPL	0.7464	0.854	10	19
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-C01:17	LALPPPPPL	0.6742	0.9895	10	19
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-C08:15	LALPPPPPL	0.4905	0.9981	10	19
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-B15:07	RLYGAILQMY	0.9995	0.7326	4	14
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-B15:04	RLYGAILQMY	0.9937	0.9741	4	14
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-A02:02	ALPPPPPLPL	0.992	0.9132	11	21
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-A02:05	ALPPPPPLPL	0.9909	0.9414	11	21
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-C01:30	ALPPPPPLPL	0.9848	0.9857	11	21
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-A02:07	ALPPPPPLPL	0.9744	0.89	11	21
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-A02:01	ALPPPPPLPL	0.973	0.8663	11	21
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-B15:05	RLYGAILQMY	0.9711	0.9519	4	14
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-C01:17	ALPPPPPLPL	0.9539	0.9899	11	21
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-B07:12	ALPPPPPLPL	0.8389	0.9031	11	21
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-B07:04	ALPPPPPLPL	0.8281	0.8157	11	21
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-B42:02	ALPPPPPLPL	0.8167	0.9392	11	21
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-B42:01	ALPPPPPLPL	0.7549	0.9354	11	21
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-B56:04	ALPPPPPLPL	0.6949	0.8026	11	21
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-B39:10	ALPPPPPLPL	0.5575	0.9969	11	21
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-C01:30	LALPPPPPLPL	0.9986	0.9868	10	21
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-C01:17	LALPPPPPLPL	0.9985	0.9907	10	21
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-B56:04	LALPPPPPLPL	0.9977	0.8307	10	21
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-B07:12	LPPPPPLPLPL	0.9975	0.8324	12	23
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-B35:12	LALPPPPPLPL	0.9972	0.9873	10	21
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-B07:04	LPPPPPLPLPL	0.9895	0.7239	12	23
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-B56:04	LPPPPPLPLPL	0.9882	0.7909	12	23
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-B07:04	LALPPPPPLPL	0.9784	0.8263	10	21
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-B35:12	LPPPPPLPLPL	0.9778	0.9893	12	23
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-B42:01	LALPPPPPLPL	0.9729	0.9076	10	21
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-B42:01	LPPPPPLPLPL	0.9676	0.8754	12	23
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-A02:07	ALPPPPPLPLP	0.9635	0.8321	11	22
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-B07:12	LALPPPPPLPL	0.952	0.8979	10	21
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-B39:10	LPPPPPLPLPL	0.9403	0.9969	12	23
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-B39:10	LALPPPPPLPL	0.9288	0.9947	10	21
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-A02:07	LALPPPPPLPL	0.9188	0.8265	10	21
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-C01:02	ALPPPPPL	0.9857	0.9869	11	19
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-C01:03	ALPPPPPL	0.9787	0.985	11	19
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-C03:03	LALPPPPPL	0.9997	0.996	10	19
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-C03:04	LALPPPPPL	0.9997	0.996	10	19
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-C03:67	LALPPPPPL	0.9995	0.9943	10	19
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-C03:05	LALPPPPPL	0.9993	0.9685	10	19
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-C15:09	LALPPPPPL	0.9993	0.9832	10	19
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-C03:17	LALPPPPPL	0.9992	0.9819	10	19
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-C03:02	LALPPPPPL	0.9989	0.9924	10	19
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-C15:05	LALPPPPPL	0.9987	0.983	10	19
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-C15:02	LALPPPPPL	0.9986	0.9842	10	19
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-C04:04	LALPPPPPL	0.9977	0.9836	10	19
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-B35:22	LALPPPPPL	0.9967	0.7196	10	19
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-C16:04	LALPPPPPL	0.9963	0.9974	10	19
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-B56:05	LALPPPPPL	0.9963	0.9452	10	19
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-B78:02	LALPPPPPL	0.9959	0.9879	10	19
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-B51:13	LALPPPPPL	0.9955	0.7088	10	19
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-B51:09	LALPPPPPL	0.9953	0.9392	10	19
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-B51:14	LALPPPPPL	0.9952	0.9228	10	19
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-B15:125	LQMYLQLRF	0.995	0.904	10	19
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-B15:34	LQMYLQLRF	0.995	0.904	10	19
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-B15:33	LQMYLQLRF	0.995	0.904	10	19
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-B15:135	LQMYLQLRF	0.9946	0.8977	10	19
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-B15:27	LQMYLQLRF	0.9944	0.8961	10	19
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-B15:24	LQMYLQLRF	0.9943	0.8941	10	19
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-B15:50	LQMYLQLRF	0.9941	0.9437	10	19
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-C03:06	LALPPPPPL	0.9937	0.9961	10	19
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-B35:13	LALPPPPPL	0.9932	0.9681	10	19
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-B59:01	LALPPPPPL	0.9925	0.6619	10	19
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-B51:21	LALPPPPPL	0.9914	0.9594	10	19
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-B51:06	LALPPPPPL	0.9913	0.954	10	19
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-B55:04	LALPPPPPL	0.9913	0.7586	10	19
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-B56:02	LALPPPPPL	0.9905	0.7732	10	19
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-B07:26	LALPPPPPL	0.9892	0.8134	10	19
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-C12:03	LALPPPPPL	0.9865	0.9967	10	19
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-B35:11	LALPPPPPL	0.986	0.9761	10	19
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-C12:02	LALPPPPPL	0.9859	0.9944	10	19
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-B35:30	LALPPPPPL	0.9853	0.9412	10	19
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-B35:17	LALPPPPPL	0.9853	0.9412	10	19
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-B35:09	LALPPPPPL	0.9848	0.9907	10	19
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-C16:01	LALPPPPPL	0.9827	0.996	10	19
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-C02:02	LALPPPPPL	0.9826	0.9958	10	19
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-C02:10	LALPPPPPL	0.9826	0.9958	10	19
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-C16:02	LALPPPPPL	0.9809	0.9977	10	19
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-B51:05	LALPPPPPL	0.9807	0.8001	10	19
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-C07:04	LALPPPPPL	0.9771	0.9917	10	19
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-C08:01	LALPPPPPL	0.9755	0.999	10	19
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-B15:35	LQMYLQLRF	0.9719	0.826	10	19
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-B35:23	LALPPPPPL	0.9708	0.9672	10	19
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-B35:77	LALPPPPPL	0.9691	0.9633	10	19
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-B15:12	LQMYLQLRF	0.9662	0.843	10	19
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-B15:53	LQMYLQLRF	0.9548	0.8814	10	19
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-C17:01	LALPPPPPL	0.9487	0.9946	10	19
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-B67:01	LALPPPPPL	0.9428	0.8972	10	19
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-A30:01	MYLQLRFNK	0.9359	0.767	12	21
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-A32:01	LQMYLQLRF	0.9341	0.9695	10	19
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-B15:39	LQMYLQLRF	0.9274	0.8812	10	19
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-A74:01	ILQMYLQLR	0.9121	0.6424	9	18
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-B15:54	LQMYLQLRF	0.8935	0.868	10	19
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-B15:30	LALPPPPPL	0.8796	0.9922	10	19
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-A02:14	LALPPPPPL	0.8746	0.8487	10	19
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-A02:06	LALPPPPPL	0.871	0.8325	10	19
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-B51:29	LALPPPPPL	0.8512	0.8836	10	19
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-B15:13	LQMYLQLRF	0.8449	0.7416	10	19
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-B07:13	LALPPPPPL	0.8349	0.9419	10	19
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-B15:73	LQMYLQLRF	0.8192	0.8107	10	19
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-B15:20	LQMYLQLRF	0.8178	0.9651	10	19
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-B15:68	LQMYLQLRF	0.8043	0.5938	10	19
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-B35:28	LQMYLQLRF	0.7857	0.9664	10	19
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-C04:03	LALPPPPPL	0.7742	0.9862	10	19
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-B48:05	LALPPPPPL	0.7454	0.6788	10	19
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-A69:01	LALPPPPPL	0.7004	0.8299	10	19
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-B18:04	LQMYLQLRF	0.659	0.9432	10	19
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-B48:02	LQMYLQLRF	0.6568	0.9594	10	19
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-C01:02	LALPPPPPL	0.6339	0.9889	10	19
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-C01:03	LALPPPPPL	0.6047	0.9904	10	19
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-B40:21	LALPPPPPL	0.5156	0.7361	10	19
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-B82:02	LALPPPPPL	0.5104	0.8137	10	19
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-C08:02	LALPPPPPL	0.4905	0.9981	10	19
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-B49:03	GQQPPLALP	0.3858	0.5256	5	14
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-C14:02	LYGAILQMY	0.0038	0.9658	5	14
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-C14:03	LYGAILQMY	0.0038	0.9658	5	14
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-B15:33	RLYGAILQMY	0.9999	0.958	4	14
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-B15:27	RLYGAILQMY	0.9999	0.9701	4	14
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-B15:50	RLYGAILQMY	0.9999	0.9494	4	14
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-B15:125	RLYGAILQMY	0.9999	0.958	4	14
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-B15:34	RLYGAILQMY	0.9999	0.958	4	14
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-B15:135	RLYGAILQMY	0.9998	0.9727	4	14
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-B15:12	RLYGAILQMY	0.9997	0.9608	4	14
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-B15:35	RLYGAILQMY	0.9995	0.939	4	14
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-B15:24	RLYGAILQMY	0.9995	0.9783	4	14
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-B15:39	RLYGAILQMY	0.9975	0.9335	4	14
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-A02:03	ALPPPPPLPL	0.9947	0.9276	11	21
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-B15:30	ALPPPPPLPL	0.985	0.9966	11	21
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-C01:03	ALPPPPPLPL	0.9817	0.9898	11	21
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-A02:14	ALPPPPPLPL	0.976	0.9216	11	21
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-A02:06	ALPPPPPLPL	0.9752	0.9032	11	21
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-A32:01	RLYGAILQMY	0.9743	0.9641	4	14
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-B15:20	RLYGAILQMY	0.974	0.97	4	14
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-C01:02	ALPPPPPLPL	0.9714	0.9895	11	21
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-B35:28	RLYGAILQMY	0.9612	0.9716	4	14
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-A74:01	RLYGAILQMY	0.9263	0.6368	4	14
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-B07:26	ALPPPPPLPL	0.9221	0.8747	11	21
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-B55:04	ALPPPPPLPL	0.8236	0.8519	11	21
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-B07:09	ALPPPPPLPL	0.8175	0.7998	11	21
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-B07:22	ALPPPPPLPL	0.8132	0.7626	11	21
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-B15:68	RLYGAILQMY	0.8032	0.6688	4	14
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-B56:02	ALPPPPPLPL	0.6949	0.8026	11	21
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-B35:22	ALPPPPPLPL	0.6754	0.7506	11	21
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-B82:02	ALPPPPPLPL	0.6723	0.844	11	21
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-B67:01	ALPPPPPLPL	0.6159	0.9817	11	21
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-B40:21	ALPPPPPLPL	0.377	0.8225	11	21
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-C03:05	LALPPPPPLPL	0.9998	0.9764	10	21
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-C03:04	LALPPPPPLPL	0.9998	0.9965	10	21
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-C03:03	LALPPPPPLPL	0.9998	0.9965	10	21
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-B07:22	LPPPPPLPLPL	0.9994	0.6764	12	23
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-B35:22	LALPPPPPLPL	0.9989	0.7725	10	21
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-B07:26	LALPPPPPLPL	0.9986	0.8758	10	21
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-C01:02	LALPPPPPLPL	0.9985	0.9905	10	21
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-B51:06	LALPPPPPLPL	0.9984	0.9709	10	21
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-C01:03	LALPPPPPLPL	0.9983	0.994	10	21
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-B55:04	LALPPPPPLPL	0.9981	0.7905	10	21
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-B56:02	LALPPPPPLPL	0.9977	0.8307	10	21
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-B51:05	LALPPPPPLPL	0.9974	0.8213	10	21
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-B35:09	LALPPPPPLPL	0.9972	0.9873	10	21
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-A02:03	RLYGAILQMYL	0.9967	0.617	4	15
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-A32:01	RLYGAILQMYL	0.9938	0.9494	4	15
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-B35:22	LPPPPPLPLPL	0.9937	0.7413	12	23
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-B55:04	LPPPPPLPLPL	0.9936	0.9062	12	23
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-B07:26	LPPPPPLPLPL	0.9914	0.825	12	23
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-B51:06	LPPPPPLPLPL	0.9909	0.8427	12	23
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-B56:02	LPPPPPLPLPL	0.9882	0.7909	12	23
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-B51:05	LPPPPPLPLPL	0.9805	0.6288	12	23
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-B35:09	LPPPPPLPLPL	0.9778	0.9893	12	23
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-B67:01	LPPPPPLPLPL	0.956	0.9861	12	23
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-B67:01	LALPPPPPLPL	0.9493	0.9468	10	21
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-B07:22	LALPPPPPLPL	0.9259	0.7742	10	21
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-B82:02	LPPPPPLPLPL	0.8806	0.7139	12	23
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	HLA-B82:02	LALPPPPPLPL	0.814	0.9349	10	21

Top

Potential FusionNeoAntigen Information of CHKA-SLC5A12 in HLA II

CHKA-SLC5A12_67864486_26734253.msa

Potential FusionNeoAntigen Information
* We used NetMHCIIpan v4.1 (%rank<0.5).

Fusion gene	Hchr	Hbp	Tgene	Tchr	Tbp	HLA II	FusionNeoAntigen peptide	Neoantigen start (at BP 13)	Neoantigen end (at BP 13)
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	DRB1-0303	QMYLQLRFNKPVRYA	11	26
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	DRB1-0338	QMYLQLRFNKPVRYA	11	26
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	DRB3-0301	QMYLQLRFNKPVRYA	11	26
CHKA-SLC5A12	chr11	67864486	chr11	26734253	489	DRB3-0303	QMYLQLRFNKPVRYA	11	26

Top

Fusion breakpoint peptide structures of CHKA-SLC5A12

3D structures of the fusion breakpoint peptide of 14AA sequence that have potential fusion neoantigens
* The minimum length of the amino acid sequence in RoseTTAFold is 14AA. Here, we predicted the 14AA fusion protein breakpoint sequence not the fusion neoantigen peptide, which is shorter than 14 AA.

File name	BPseq	Hgene	Tgene	Hchr	Hbp	Tchr	Tbp	AAlen
7514	QQPPLALPPPPPLP	CHKA	SLC5A12	chr11	67864486	chr11	26734253	489

File name	BPseq	Hgene	Tgene	Hchr	Hbp	Tchr	Tbp	AAlen
10638	YGAILQMYLQLRFN	CHKA	SLC5A12	chr11	67864486	chr11	26734253	489

Top

Filtering FusionNeoAntigens Through Checking the Interaction with HLAs in 3D of CHKA-SLC5A12

Virtual screening between 25 HLAs (from PDB) and FusionNeoAntigens
* We used Glide to predict the interaction between HLAs and neoantigens.

HLA allele	PDB ID	File name	BPseq	Docking score	Glide score
HLA-B14:02	3BVN	7514	QQPPLALPPPPPLP	-7.15543	-7.26883
HLA-B14:02	3BVN	7514	QQPPLALPPPPPLP	-4.77435	-5.80965
HLA-B52:01	3W39	7514	QQPPLALPPPPPLP	-6.80875	-6.92215
HLA-B52:01	3W39	7514	QQPPLALPPPPPLP	-4.20386	-5.23916
HLA-A11:01	4UQ2	7514	QQPPLALPPPPPLP	-7.5194	-8.5547
HLA-A11:01	4UQ2	7514	QQPPLALPPPPPLP	-6.9601	-7.0735
HLA-A24:02	5HGA	7514	QQPPLALPPPPPLP	-7.52403	-7.63743
HLA-A24:02	5HGA	7514	QQPPLALPPPPPLP	-5.82433	-6.85963
HLA-B27:05	6PYJ	7514	QQPPLALPPPPPLP	-3.28285	-4.31815
HLA-B44:05	3DX8	7514	QQPPLALPPPPPLP	-5.91172	-6.94702
HLA-B44:05	3DX8	7514	QQPPLALPPPPPLP	-4.24346	-4.35686
HLA-B14:02	3BVN	10638	YGAILQMYLQLRFN	-7.15543	-7.26883
HLA-B14:02	3BVN	10638	YGAILQMYLQLRFN	-4.77435	-5.80965
HLA-B52:01	3W39	10638	YGAILQMYLQLRFN	-6.80875	-6.92215
HLA-B52:01	3W39	10638	YGAILQMYLQLRFN	-4.20386	-5.23916
HLA-A11:01	4UQ2	10638	YGAILQMYLQLRFN	-7.5194	-8.5547
HLA-A11:01	4UQ2	10638	YGAILQMYLQLRFN	-6.9601	-7.0735
HLA-A24:02	5HGA	10638	YGAILQMYLQLRFN	-7.52403	-7.63743
HLA-A24:02	5HGA	10638	YGAILQMYLQLRFN	-5.82433	-6.85963
HLA-B27:05	6PYJ	10638	YGAILQMYLQLRFN	-3.28285	-4.31815
HLA-B44:05	3DX8	10638	YGAILQMYLQLRFN	-5.91172	-6.94702
HLA-B44:05	3DX8	10638	YGAILQMYLQLRFN	-4.24346	-4.35686

Top

Vaccine Design for the FusionNeoAntigens of CHKA-SLC5A12

mRNA and peptide sequences of FusionNeoAntigens that have potential interaction with HLA-Is.

Fusion gene	Hchr	Hbp	Tchr	Tbp	Start in +/-13AA	End in +/-13AA	FusionNeoAntigen peptide sequence	FusionNeoAntigen RNA sequence
CHKA-SLC5A12	chr11	67864486	chr11	26734253	10	19	LALPPPPPL	TTGCAGATGTACTTACAACTACGATTC
CHKA-SLC5A12	chr11	67864486	chr11	26734253	10	19	LQMYLQLRF	TTGCAGATGTACTTACAACTACGATTC
CHKA-SLC5A12	chr11	67864486	chr11	26734253	10	21	LALPPPPPLPL	TTGCAGATGTACTTACAACTACGATTCAACAAA
CHKA-SLC5A12	chr11	67864486	chr11	26734253	11	19	ALPPPPPL	CAGATGTACTTACAACTACGATTC
CHKA-SLC5A12	chr11	67864486	chr11	26734253	11	20	ALPPPPPLP	CAGATGTACTTACAACTACGATTCAAC
CHKA-SLC5A12	chr11	67864486	chr11	26734253	11	21	ALPPPPPLPL	CAGATGTACTTACAACTACGATTCAACAAA
CHKA-SLC5A12	chr11	67864486	chr11	26734253	11	22	ALPPPPPLPLP	CAGATGTACTTACAACTACGATTCAACAAACCA
CHKA-SLC5A12	chr11	67864486	chr11	26734253	12	21	MYLQLRFNK	ATGTACTTACAACTACGATTCAACAAA
CHKA-SLC5A12	chr11	67864486	chr11	26734253	12	23	LPPPPPLPLPL	ATGTACTTACAACTACGATTCAACAAACCAGTT
CHKA-SLC5A12	chr11	67864486	chr11	26734253	3	14	LRLYGAILQMY	CTGCGGCTGTATGGAGCGATTTTGCAGATGTAC
CHKA-SLC5A12	chr11	67864486	chr11	26734253	4	14	RLYGAILQMY	CGGCTGTATGGAGCGATTTTGCAGATGTAC
CHKA-SLC5A12	chr11	67864486	chr11	26734253	4	15	RLYGAILQMYL	CGGCTGTATGGAGCGATTTTGCAGATGTACTTA
CHKA-SLC5A12	chr11	67864486	chr11	26734253	5	14	GQQPPLALP	CTGTATGGAGCGATTTTGCAGATGTAC
CHKA-SLC5A12	chr11	67864486	chr11	26734253	5	14	LYGAILQMY	CTGTATGGAGCGATTTTGCAGATGTAC
CHKA-SLC5A12	chr11	67864486	chr11	26734253	8	17	AILQMYLQL	GCGATTTTGCAGATGTACTTACAACTA
CHKA-SLC5A12	chr11	67864486	chr11	26734253	9	18	ILQMYLQLR	ATTTTGCAGATGTACTTACAACTACGA

mRNA and peptide sequences of FusionNeoAntigens that have potential interaction with HLA-IIs.

Fusion gene	Hchr	Hbp	Tchr	Tbp	Start in +/-13AA	End in +/-13AA	FusionNeoAntigen peptide	FusionNEoAntigen RNA sequence
CHKA-SLC5A12	chr11	67864486	chr11	26734253	11	26	QMYLQLRFNKPVRYA	CAGATGTACTTACAACTACGATTCAACAAACCAGTTCGCTATGCT

Top

Information of the samples that have these potential fusion neoantigens of CHKA-SLC5A12

These samples were reported as having these fusion breakpoints. For individual breakpoints, we checked the open reading frames considering multiple gene isoforms and chose the in-frame fusion genes only. Then, we made fusion protein sequences and predicted the fusion neoantigens. These fusion-positive samples may have these potential fusion neoantigens.

Cancer type	Fusion gene	Hchr	Hbp	Henst	Tchr	Tbp	Tenst	Sample
BRCA	CHKA-SLC5A12	chr11	67864486	ENST00000265689	chr11	26734253	ENST00000280467	TCGA-C8-A1HK-01A
BRCA	CHKA-SLC5A12	chr11	67864486	ENST00000265689	chr11	26734253	ENST00000396005	TCGA-C8-A1HK-01A

Top

Potential target of CAR-T therapy development for CHKA-SLC5A12

Predicted 3D structure. We used RoseTTAFold.

Retention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, to provide the retention of the transmembrane domain, we only show the protein feature retention information of those transmembrane features

* Minus value of BPloci means that the break point is located before the CDS.

- In-frame and retained 'Transmembrane'.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Protein feature

Protein feature note

Tgene

SLC5A12

chr11:67864486

chr11:26734253

ENST00000280467

129_149

397.0

Transmembrane

Helical

Tgene

SLC5A12

chr11:67864486

chr11:26734253

ENST00000280467

158_178

397.0

Transmembrane

Helical

Tgene

SLC5A12

chr11:67864486

chr11:26734253

ENST00000280467

181_201

397.0

Transmembrane

Helical

Tgene

SLC5A12

chr11:67864486

chr11:26734253

ENST00000280467

236_256

397.0

Transmembrane

Helical

Tgene

SLC5A12

chr11:67864486

chr11:26734253

ENST00000280467

276_296

397.0

Transmembrane

Helical

Tgene

SLC5A12

chr11:67864486

chr11:26734253

ENST00000280467

322_342

397.0

Transmembrane

Helical

Tgene

SLC5A12

chr11:67864486

chr11:26734253

ENST00000280467

386_406

397.0

Transmembrane

Helical

Tgene

SLC5A12

chr11:67864486

chr11:26734253

ENST00000280467

412_432

397.0

Transmembrane

Helical

Tgene

SLC5A12

chr11:67864486

chr11:26734253

ENST00000280467

438_458

397.0

Transmembrane

Helical

Tgene

SLC5A12

chr11:67864486

chr11:26734253

ENST00000280467

505_525

397.0

Transmembrane

Helical

Tgene

SLC5A12

chr11:67864486

chr11:26734253

ENST00000396005

129_149

619.0

Transmembrane

Helical

Tgene

SLC5A12

chr11:67864486

chr11:26734253

ENST00000396005

158_178

619.0

Transmembrane

Helical

Tgene

SLC5A12

chr11:67864486

chr11:26734253

ENST00000396005

181_201

619.0

Transmembrane

Helical

Tgene

SLC5A12

chr11:67864486

chr11:26734253

ENST00000396005

236_256

619.0

Transmembrane

Helical

Tgene

SLC5A12

chr11:67864486

chr11:26734253

ENST00000396005

276_296

619.0

Transmembrane

Helical

Tgene

SLC5A12

chr11:67864486

chr11:26734253

ENST00000396005

322_342

619.0

Transmembrane

Helical

Tgene

SLC5A12

chr11:67864486

chr11:26734253

ENST00000396005

386_406

619.0

Transmembrane

Helical

Tgene

SLC5A12

chr11:67864486

chr11:26734253

ENST00000396005

412_432

619.0

Transmembrane

Helical

Tgene

SLC5A12

chr11:67864486

chr11:26734253

ENST00000396005

438_458

619.0

Transmembrane

Helical

Tgene

SLC5A12

chr11:67864486

chr11:26734253

ENST00000396005

505_525

619.0

Transmembrane

Helical

Subcellular localization prediction of the transmembrane domain retained fusion proteins
* We used DeepLoc 1.0. The order of the X-axis of the barplot is as follows: Entry_ID, Localization, Type, Nucleus, Cytoplasm, Extracellular, Mitochondrion, Cell_membrane, Endoplasmic_reticulum, Plastid, Golgi.apparatus, Lysosome.Vacuole, Peroxisome. Y-axis is the output score of DeepLoc. Clicking the image will open a new tab with a large image.

Hgene

Hchr

Hbp

Henst

Tgene

Tchr

Tbp

Tenst

DeepLoc result

Top

Related Drugs to CHKA-SLC5A12

Drugs used for this fusion-positive patient.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)

Hgene

Tgene

Drug

Source

PMID

Top

Related Diseases to CHKA-SLC5A12

Diseases that have this fusion gene.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)

Hgene

Tgene

Disease

Source

PMID

Diseases associated with fusion partners.
(DisGeNet 4.0)

Partner

Gene

Disease ID

Disease name

# pubmeds

Source

	Fusion Gene and Fusion Protein Summary
	Fusion Amino Acid Sequences (multiple BPs and multiple gene isoforms)
	Fusion Protein Breakpoint Sequences - (for the Screening of the FusionNeoAntigens)
	Potential FusionNeoAntigens in HLA I - (netMHCpan v4.1 + deepHLApan v1.1)
	Potential FusionNeoAntigens in HLA II - (netMHCIIpan v4.1)
	Fusion Breakpoint 14 AA Peptide Structure - (RoseTTAFold)
	Filtering FusionNeoAntigens Through Checking the Interaction with HLAs in 3D - (Glide)
	Vaccine Design for the FusionNeoAntigens (RNA/protein sequences)
	Potential target of CAR-T therapy development
	Information on the samples that have these potential fusion neoantigens
	Fusion Protein Targeting Drugs - (Manual Curation)
	Fusion Protein Related diseases - (Manual Curation)